LOCUS       DASVKP010000001         7553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1068879, whole genome shotgun sequence.
ACCESSION   DASVKP010000001 DASVKP010000000
VERSION     DASVKP010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068879"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VF362_00005"
     CDS             complement(<1..609)
                     /locus_tag="VF362_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589345.1"
                     /translation="MSGRDPELHRARERIQRSLYMATGFGALIFGGMLAGRISSQWSE
                     LNASFAILAVLVCVAIPATLTGLAWILPLRVLDAMAGGTVVAFVVLQLLWVPSMVAPH
                     LANGAAPWMQGVNAAQATLAAIVWRHRLVWLYAVAQGPIVATVRYLATGSDVRAALLD
                     GLGAIVFCTILMAVAIALINAAQQQDLVAARARAQASLEAGSR"
     gene            complement(606..1247)
                     /locus_tag="VF362_00010"
     CDS             complement(606..1247)
                     /locus_tag="VF362_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7589346.1"
                     /translation="MDAPRTIALVEDHALIALGFRDLVASAADLRLVGMVTTVVDLEA
                     IAEHIDLVVLDLRLNDGSSPADNVAAVHRRGSQVLIYTGAEDRRLIQQAARSGALGLI
                     RKSAEPGVLLDAIRTAAAGREVFGADWAAAIDADDELQDARLSPREQEVLSLYASGET
                     ATSVAQRTGLARETVADYVTRIRKKYAEAGRPAHSRVDLFRRALEDGLVEGPE"
     gene            1418..1837
                     /locus_tag="VF362_00015"
     CDS             1418..1837
                     /locus_tag="VF362_00015"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HEX7589347.1"
                     /translation="MRVLRFAAAGIALAGILSGCSSRPVGDLKVGECFDFSEATGEVT
                     AVAVVECAKPHDAEVFTTFEVALDSFDADTVAAAADDQCLAAIEPYVGYPYSAADFYY
                     RAFMPGADSWGKGARTIVCFVVPNEGTLTGSVKKSAA"
     gene            complement(1834..2442)
                     /locus_tag="VF362_00020"
     CDS             complement(1834..2442)
                     /locus_tag="VF362_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589348.1"
                     /translation="MTTPGLPPERQSLYPEPYRAGNWDTAPPKRAARAWIIPVIGAGL
                     AALALAGWAGIEVGHRVGTSDITVSDPSGQTINAIQVAPGTCLKKLPSNGTAATADAV
                     GCAVPHEAEAVADYTFTGDEWPGRSVVVSKLLDFCGSFVQPGYSDSSMFAATDWDAGL
                     RWVAWAPTERSWADNQRTGLCVVYRQGQVEGSFIDSSATFVN"
     gene            complement(2439..3251)
                     /locus_tag="VF362_00025"
     CDS             complement(2439..3251)
                     /locus_tag="VF362_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX7589349.1"
                     /translation="MRLASWNVNSIRARIDRVIAFLERANVDVLAMQEIKCRPDQFPT
                     ERFAAAGYEVACHGLHQFNGVAIASRVGLADVAESFPDQPSFGKAGDETVLEARALGA
                     TCGGVRVWSIYVPNGRTLDDPHYAYKLEFLRALARAAGPWASEGPVALVGDWNVAPLD
                     TDIWSLDAPEAATHVAPDAREALAAIERAGLEELSRRFLPEPNTYTFWDYQRLRFPRG
                     EGMRIDFAFASPALASRARTAVIDRAERKGVGASDHAPIMVDFVDAPGSVVP"
     gene            3288..4067
                     /locus_tag="VF362_00030"
     CDS             3288..4067
                     /locus_tag="VF362_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7589350.1"
                     /translation="MAWALVTGASVGLGAEFARQIAAGGRNVVLVSRDAERLGTVARE
                     LEALGVEAEVVVADLAKRADVARVARRIASEERPVDLLVNNAGFGFRHGFLEATEAEL
                     DDALDVMVRAVMILSQAAARAMVRRGSGTILNVSSIAAVLVTGTYSAHKAWVSAFTEA
                     LAGDLYGTGVTATAVCPGLVRTKFHERAALDLTALPRAVWLDAPTVVTSALRAARRRR
                     VFVTPTVRYGVIMAVARILPRRWVRRASRSGSGRKANRPST"
     gene            4095..4664
                     /gene="pyrE"
                     /locus_tag="VF362_00035"
     CDS             4095..4664
                     /gene="pyrE"
                     /locus_tag="VF362_00035"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX7589351.1"
                     /translation="MMQGSPRERLRELILSTAVIRGRVTLSSGREADYYIDLRRVTLH
                     HEGAPLVGHIMLEQLGVAGFGPGKVDAVGGLTLGADPIATALLHAAAARGRRLDAFVV
                     RKDAKTHGMQRRIEGPDVEGRRVVVVEDTSTTGASALTAVEALREAGAIVLAVAVIVD
                     RDTGAREAVEAQGLPYIYVYRLAELGLES"
     gene            4676..5680
                     /locus_tag="VF362_00040"
     CDS             4676..5680
                     /locus_tag="VF362_00040"
                     /inference="COORDINATES: protein motif:HMM:NF022779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HEX7589352.1"
                     /translation="MSPRSVISAAVGALSVVAILLLNTGIVAIAGPWVMAAIVGLLVV
                     AVGWFAWDLWSQGRHRRAMMRFADHIDGWEYRPQTRDYDERFLTFPFGVGRDLRDVDL
                     IRGPFNGFACASFTHEYEEGDKDEFNATSRWQIDVVDLPYPLAKVDIVPDDFLAKFAK
                     LLGGQDIDFESAQFNARWRVKAGDAKYAHDIVHPRLMERLLEADADGLAIRIEGSAVY
                     SWAVDRRGPEDLARRLGVLTAVARSIPDFVYREFKEVHDRLAEEQRKREENAPEWAKT
                     PFALSGGRYTDLGREDYREMELSRFGDQGGKPPSDGGPGGGAGHWDAPPDDPNRVRRG
                     "
     gene            5756..6313
                     /locus_tag="VF362_00045"
     CDS             5756..6313
                     /locus_tag="VF362_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX7589353.1"
                     /translation="MPYVIAIIVVIAIFALWAVAQYNGLVKLRNLVQEAWRQIDVELQ
                     RRHDLIPNLVETVKGYATHEKGTFEEVTRARAAAASPGSGPAEQAAQENVLTQALGKL
                     FAVVENYPDLKANVNFQQLQAELTNTEDRIAAGRRFYNANVRTLNTKVETFPTNIIAG
                     MFGFKQAEYFETTDEATRQAPAVSF"
     gene            complement(6383..7039)
                     /locus_tag="VF362_00050"
     CDS             complement(6383..7039)
                     /locus_tag="VF362_00050"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX7589354.1"
                     /translation="MTTLLHTLGPSWMDPETLLNWFGSWAVWGVAAVEFIETGLLFPI
                     LPGDTLLFTAGVFTANNTITMPIALVATITGIAAILGPQSGYLIGRLAGPKIFARRDG
                     RIFKKANVAKTHAFFEKYGGRALILGQFVPFVRTYIPVAAGVGKMPYRHFAMFNVVGA
                     MTWGVGVPLLGFSLGSVAFIKDHYEIAVLLVLFVSLLPIIVEWIRHKFEQRRGAKAST
                     "
     gene            7132..>7553
                     /locus_tag="VF362_00055"
     CDS             7132..>7553
                     /locus_tag="VF362_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589355.1"
                     /translation="MDGQLAAAATFVAHARAWRAGRAPKPAYAHLTAFRHAHELYFDT
                     PVGLVIPGHERFRPKDVSRWLDWIPRAGPRDLRVGVVGGVIRDADLPEKAHMATFSPF
                     RFTVVDTRGRMTAWVSEREPVTTTQDRRGLWVVHFRHV"
BASE COUNT         1221 a         2503 c         2648 g         1181 t
ORIGIN      
        1 cctgctgccg gcctcgagcg aggcctgcgc ccgtgctcgc gcggctacga ggtcctgctg
       61 ctgcgcggcg ttgatgagcg cgatggcaac ggccatgagg atcgtgcaga acacgatggc
      121 gccgagcccg tcgagcagtg ccgccctcac gtcactcccg gtggcgaggt agcgcaccgt
      181 cgcgacgatg gggccctggg cgacggcgta gagccacacg agtctgtggc gccacacgat
      241 cgcggccagc gttgcctgcg cggcgttgac gccctgcatc cagggagccg ccccgttggc
      301 gaggtgcggc gcgaccatcg acggaaccca caggagttgc agcacgacga acgcgacgac
      361 ggtgccgccc gccatcgcgt cgagaaccct gaggggaagg atccacgcga gcccggtgag
      421 ggtcgcggga atcgcgacgc agacgaggac cgccaagatg gcgaacgacg cgttgagttc
      481 cgaccattgc gaggagatcc ttccggcgag cattccgccg aagatgagcg ccccgaagcc
      541 cgtcgccatg tacagggagc gctgaatccg ttctctcgct cggtgaagct cggggtcgcg
      601 gccgctcact cgggtccctc caccaggccg tcttcgagcg ctcgccggaa gaggtcgacg
      661 cgcgagtgcg cgggcctgcc agcctccgcg tacttcttgc gtattcgggt gacgtagtcg
      721 gcgacggttt cccgcgccaa gcccgtcctt tgcgcgaccg acgtcgccgt ctcgcccgag
      781 gcgtagaggc tcaggacctc ctgctcccgc gggctcaggc gcgcgtcctg caattcgtcg
      841 tcggcgtcga tcgccgccgc ccagtcggcg ccgaagacct cgcggccagc ggcggcggtc
      901 cggatcgcgt cgaggagaac cccgggctcc gccgacttgc gaatgagccc cagcgcgccg
      961 cttcgagcag cttgctggat gaggcggcgg tcctccgccc cggtgtagat caggacctgg
     1021 gaccccctgc gatgcacggc cgcgacgttg tcggcgggcg aagacccgtc gttcaatcga
     1081 aggtcgagga cgacgagatc gatgtgctcg gcaatggcct caaggtcaac cacggtggtc
     1141 accatgccga ccagccgaag gtcggctgcg gaggccacga ggtcgcggaa gcccagggcg
     1201 atgagcgcgt ggtcctcgac caacgcgatc gtgcgtggcg catccatgat cctccatccg
     1261 tcccgtggac tcaccgtacc caaaagcggc caacgtgcgc atccgggatt ggggcgcaat
     1321 tcatcacatt ctgcggaatg cctcgcgagc aacgttccgt cgatccgact agcctgaact
     1381 cggagggagc gaatcgctcc caggacggaa gcgtgcgatg cgagttctgc gattcgcggc
     1441 cgcgggcatc gcgctcgcgg ggatcctatc gggatgcagt tcgcgtcccg tgggcgacct
     1501 caaggttggc gagtgcttcg acttctcgga ggcgacgggc gaggtgacgg ccgtcgccgt
     1561 cgtggagtgc gcgaagccgc acgacgccga ggtgttcacg acgttcgagg tcgcccttga
     1621 ctcgtttgac gccgataccg tggccgccgc ggccgacgac cagtgcctcg ccgcgatcga
     1681 gccctacgtt gggtatccct atagtgcggc ggacttctac taccgggcgt tcatgccggg
     1741 cgccgacagt tgggggaagg gtgcccgaac catcgtgtgc ttcgtcgtcc ccaacgaggg
     1801 gacgctcacg gggagcgtca agaagtccgc agcctagttg acgaaggtcg cagaagagtc
     1861 gatgaacgac ccttcgacct ggccctgccg gtagacgacg cagaggccgg tgcgctgatt
     1921 gtccgcccag ctgcgctccg tgggcgccca cgcgacccac cggagtccgg catcccagtc
     1981 ggtcgcggca aacatcgacg agtccgagta cccgggttgc acgaaggatc cgcagaagtc
     2041 cagaagcttc gagaccacga cggaccttcc gggccactcg tccccggtga aggtgtagtc
     2101 ggcaaccgcc tccgcctcgt gcggcaccgc acacccgacg gcgtccgcgg tcgccgcggt
     2161 cccgttggaa ggaagtttct tgaggcacgt cccgggggct acctggatcg cgttgatcgt
     2221 ctgaccggag ggatcgctga ccgtgatgtc cgatgtgccg actcgatgtc cgacctctat
     2281 ccccgcccag ccggcgaggg cgagcgcggc gagccccgcc ccgatgacgg gaatgatcca
     2341 ggcgcgcgcg gcgcgcttcg ggggtgccgt gtcccagttc ccggcgcggt agggctcggg
     2401 gtagagactc tgccgctcgg gcgggaggcc aggcgtcgtc atggcaccac gctaccggga
     2461 gcgtcgacga agtccaccat gatcggcgcg tgatccgatg cgccgacacc cttgcgctct
     2521 gcgcggtcga tgacggcggt gcgcgcgcgg gaggcgagcg cgggcgaggc gaaggcgaag
     2581 tcgatgcgca tcccctcccc ccgagggaac cggagtcgct gatagtccca gaaggtgtag
     2641 gtattgggct cgggaaggaa tcggcgcgaa agctcttcaa gccccgcgcg ttcgatcgcc
     2701 gccagtgcct ctcgcgcgtc cggcgcgaca tgcgtcgccg cctcgggggc atcgagcgac
     2761 cagatgtccg tgtcgagggg cgcgacgttc cagtcgccga cgagcgcgac cggaccttcg
     2821 gaagcccatg gcccggccgc gcgagcgagc gcgcgtagga attccagctt gtacgcgtag
     2881 tgcgggtcgt cgagcgtcct cccgttggga acgtagatgc tccacacgcg cactccgcca
     2941 cacgtcgcgc cgagcgcgcg cgcctcgagt accgtctcgt cgcccgcctt gccgaatgac
     3001 ggttggtcgg ggaacgactc ggcgacatcg gcgagcccga cgcgcgacgc gatcgcgacc
     3061 ccgttgaact ggtgcaggcc gtggcacgcg acctcgtagc ccgccgccgc gaaccgttcc
     3121 gtcgggaact gatccggtcg gcacttgatc tcctgcatcg cgagcacatc gacgtttgcc
     3181 cgctcaagga aggcgatgac acggtcgatg cgggcgcgga tcgagttgac attccatgac
     3241 gcgaggcgca ttgctccacg ctacccgcgc cgccgtaggc tggccgcatg gcatgggcgt
     3301 tggtgacggg ggcgagtgtg gggctcggcg cggaattcgc gcggcagatc gcggcgggcg
     3361 gcaggaacgt cgtcctcgta tcgcgggatg cggaacggct cggcaccgtg gctcgcgaac
     3421 ttgaagcgct tggcgtcgag gccgaggtgg tggtggccga cctcgcgaag cgcgccgatg
     3481 tcgctcgagt cgcgcggcgc atcgcatccg aggaacggcc cgtcgacctc ctggtcaaca
     3541 acgcgggctt cggatttcgg cacggattcc tcgaggcgac cgaggcggaa ctggacgacg
     3601 ccctcgacgt gatggtccgc gcggtgatga tcctgtcgca agcggcggcg cgcgcgatgg
     3661 tccggcgagg gtcggggacc atcctcaacg tctcatcgat cgctgcggtg ctcgtcacgg
     3721 ggacgtattc ggcgcacaag gcctgggtgt cggcgttcac ggaggcgctc gccggggatc
     3781 tctacggcac gggggtcacc gccaccgccg tctgccccgg gttggtccga accaaatttc
     3841 acgagcgcgc cgcgcttgat ctgaccgccc tgccccgcgc ggtgtggctc gacgcgccaa
     3901 cggtcgtgac atcggcgctt cgcgcggcgc gccgaaggcg cgtcttcgtg acccccacgg
     3961 tccgctacgg cgtgatcatg gccgttgccc gcatcctgcc ccggcggtgg gtccgtcgcg
     4021 cgtctcgctc gggctcggga cggaaggcga accggccctc aacttaggag ggcatgcgct
     4081 agtaggctgg cgacatgatg caaggtagcc cacgcgaacg acttcgcgag ttgattctca
     4141 gtacggcggt catccgcgga cgcgtcaccc tctcctcggg gcgggaggcc gactactaca
     4201 tcgatctgcg ccgggtgacc ctccaccatg agggagcgcc gctcgtgggg cacatcatgc
     4261 tcgagcaact cggcgttgct ggtttcggcc ccggcaaggt cgacgcggtc ggtgggctca
     4321 cgctgggcgc cgaccccatc gcgaccgccc tgcttcacgc cgccgcggcc cgcggcagga
     4381 ggctcgatgc cttcgtcgtc cgcaaggacg cgaagaccca cgggatgcaa cgccgcatcg
     4441 aaggcccgga cgtcgagggc cgtcgcgtcg tcgtggtcga ggacacctcg acgacggggg
     4501 cgtccgcgct cacggccgtc gaggcgttgc gcgaggcggg agccatcgtc ctggcggtcg
     4561 cggtgatcgt ggaccgcgac accggggccc gcgaggcggt tgaggctcag ggccttccct
     4621 acatctacgt ctaccgactc gcggagttgg gcctcgagtc ctgatgaact accggatgtc
     4681 cccgcggagc gtcatctccg ccgcggtcgg cgcgctgagt gtggtcgcca tccttttgct
     4741 caacacgggc atcgtcgcga tcgcggggcc gtgggtgatg gcagcgattg tgggcctcct
     4801 cgtcgttgcg gtcgggtggt tcgcgtggga cctctggtcg cagggacgtc accgccgagc
     4861 catgatgcgc ttcgccgacc acatcgacgg gtgggaatac aggccccaga ccagggacta
     4921 cgacgagcga ttcctcacct tcccgttcgg cgtcggtcgt gacctgcgcg acgtcgacct
     4981 gattcgcggc ccgttcaacg ggttcgcgtg cgcctcgttt acccacgagt atgaggaggg
     5041 cgacaaggat gagttcaacg cgacgagccg gtggcagatt gacgtcgtcg acctgccgta
     5101 cccgctcgcg aaggtcgaca tcgtgcccga cgacttcctg gccaagttcg ccaagcttct
     5161 cggcggccag gacatcgact ttgagagcgc gcagttcaac gcgcggtgga gggtcaaggc
     5221 gggcgacgcg aagtacgccc acgacatcgt ccacccgcgg ctgatggagc gcctcctcga
     5281 ggcggacgcg gacgggctgg cgattcgcat cgagggttcc gccgtctact cctgggcggt
     5341 cgaccgacgc ggcccggaag atctcgcgcg ccgattgggc gtgctgactg cggtcgcccg
     5401 gtcgattccc gacttcgtgt accgggagtt caaggaggtt cacgatcgcc tcgccgagga
     5461 gcagcgcaag cgcgaggaga acgcgccgga atgggccaag acgcccttcg ccctgtccgg
     5521 cgggcgctac acggacctgg ggcgagagga ctaccgcgag atggaactct cccgctttgg
     5581 cgaccagggg gggaagccgc cgagcgacgg gggtcccggc gggggagcgg ggcactggga
     5641 cgcgcctccc gacgatccca accgcgtcag acgcggctag gcaccgcaac ggtgggcatt
     5701 tggagccggg cccgctagca tcgcgatagg accgagaccc tgaggagggt tcgacatgcc
     5761 gtacgttatc gccatcatcg ttgtcatagc gatcttcgcg ctgtgggccg tcgcccagta
     5821 caacgggctc gtcaagctca ggaacctggt ccaggaggcc tggcgccaga ttgacgtcga
     5881 gctccagcgg cgacacgacc tcattcccaa ccttgtcgag acggtcaagg gctacgcaac
     5941 ccacgagaag ggcaccttcg aagaggtgac gagggcgcgc gccgcggccg cttctccggg
     6001 ctcaggcccg gccgagcagg ccgcccagga gaacgtcctg acccaggcac tcggcaagct
     6061 cttcgcggtc gtcgagaact accccgacct caaggcgaac gtcaacttcc agcagttgca
     6121 ggcggaactg acgaacaccg aggatcgcat cgccgcgggg cgccggttct acaacgcgaa
     6181 cgttcggacg ctcaacacca aggtggagac cttccccacg aacatcatcg cgggcatgtt
     6241 cggcttcaag caagcggaat acttcgagac gacggacgag gccacgcgtc aggcgcccgc
     6301 cgtcagcttc tagaccgcag gcaagaggag gggcccccgc atcggcgggg gcccctcctt
     6361 ccttgctggg gtgacgtgcg cgtcaggtgg aggccttcgc gccccggcgc tgctcgaact
     6421 tgtggcggat ccactcgacg atgatgggaa gcagcgagac gaagagcacc aggagcacgg
     6481 cgatctcgta gtggtccttg ataaacgcga ccgagccgag cgagaagccc aggagcggga
     6541 cgccgacgcc ccaggtcatc gcgccgacga cgttgaacat cgcgaagtgg cggtagggca
     6601 tctttcccac gcccgccgcg acggggatgt aggtgcgcac gaacgggacg aactgcccga
     6661 ggatgagcgc gcgaccccca tacttctcga agaaggcgtg ggtcttggcg acgttcgcct
     6721 tcttgaagat gcgtccgtcg cgtcgggcga agatcttcgg cccggcgagg cgcccgatca
     6781 ggtatccgct ctgcgggccc aggatcgcgg cgatgcccgt gatggtcgcg accagcgcga
     6841 tgggcatcgt gatcgtgttg ttcgccgtaa agacgcccgc agtgaagagg agggtgtccc
     6901 cggggagaat ggggaagagg aggcccgtct cgatgaactc gacggcggct acgccccaga
     6961 cggcccagga gccgaaccag ttcaggagcg tttcggggtc catccaggac ggtccgagcg
     7021 tgtgcaggag ggtagtcatc acccgcatag cctacgtccc cgacttcgcc gatccctcgg
     7081 ccgttggtgc ggtccccgtc gccggggccg ctatcgcgct agcatcgcac cgtggatggg
     7141 caactggccg ccgctgcgac gttcgtcgcg cacgcgcgtg cgtggcgggc ggggcgcgcg
     7201 cccaaacccg cgtacgcaca cctgacggcc ttccggcacg cgcacgaact ctacttcgac
     7261 actccggtgg gcctggtcat acctggccac gagcgcttcc ggcccaagga cgtcagccgg
     7321 tggctcgact ggattccccg cgcgggaccg cgcgacctta gggtcggcgt tgtgggcgga
     7381 gtcattcgcg atgcggatct tcccgagaag gcgcacatgg cgacgttttc gccattccga
     7441 ttcacggttg ttgacactcg cgggcggatg acggcgtggg tgtccgaacg ggagccggtc
     7501 acgacaacgc aggacaggag agggctctgg gtcgtgcatt ttcgccacgt cga
//
LOCUS       DASVKP010000002         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1315520, whole genome shotgun sequence.
ACCESSION   DASVKP010000002 DASVKP010000000
VERSION     DASVKP010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315520"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VF362_00060"
     CDS             complement(<1..548)
                     /locus_tag="VF362_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589356.1"
                     /translation="MADYISRALVADALSEWREQLRALAKASPLRDVGGGEIPVVDLT
                     AAHPSGLAQLFAGRPTLVSALIRDQQAQPLALRRARAACEHAETVRLNTGIATGALVV
                     GTALWSEGGSRREVPILLRHVTLEPSRETDTQVTLHTTVTLNPILATEMRARAPESAV
                     AELAAAIPQADEFDPRPVWNEVR"
     gene            862..>2000
                     /locus_tag="VF362_00065"
     CDS             862..>2000
                     /locus_tag="VF362_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589357.1"
                     /translation="MGKFFGSAGGWFIILLVALVALVSGIAFGWFDSGGSPGPGPSGT
                     ATVKSTPTSSTTATPTSFVARPVTTGPHVPSASALTADVLADSGSGWVVAIDDTSQVN
                     TLNDPNIVMPGQKILYLISPDGTRYELANLDGIGLTDPNLVAWDYARDVLLMEQAGTT
                     IGVFDMESGAMTSSWTYCPSGHGSARYGEARGGNWVVRAGCEGTGVDALYTDAGVVVP
                     STIQVAPPMVTNFDIGTMQVRYEFEGTVDKKYLAYSPDGASEPLPWSALSYCDTPLGK
                     GREDTLAVLCYSDAGKISIEELPVNGSPPYEVVSTAQLDGFAMAHGGFGPGEYGLTGY
                     WSDSALGIVQFWFGDANSRLGVLKGGTVAAVGADASYPFRRVHAVS"
BASE COUNT          303 a          702 c          693 g          302 t
ORIGIN      
        1 cgcacctcgt tccacaccgg gcgggggtcg aactcgtcgg cctgggggat cgccgcggca
       61 agttcggcga ccgcagactc gggggcgcgg gcccgcatct ccgtcgcgag gattgggttc
      121 agggtgacgg tcgtgtgaag cgtcacctga gtgtccgtct cgcgcgacgg ctcgagcgtg
      181 acgtgcctca ggagaatggg aacctcgcga cgggaaccgc cctcggacca cagcgccgtc
      241 ccgacgacga gggcgcccgt ggcaatgccc gtattgagcc gcacggtctc ggcgtgctcg
      301 cacgcggccc tcgcgcgtcg aagggcgagc ggttgcgcct gctggtcgcg aatgagggcg
      361 gagaccaggg tcgggcgacc ggcgaacaac tgggcaagcc cgctcggatg ggctgcggtc
      421 aggtccacca cggggatctc gccgccgccg acgtcgcgga gcggcgaggc cttcgcaagc
      481 gctcgcaact gctcccgcca ctccgagagc gcgtcggcga caagcgcgcg cgagatatag
      541 tccgccactc ccctagcgta gggatgcgag gtggtcgccc cccggctgcc acgccgctgg
      601 acggggccgg gtgccggaga agtgcacgac agtgcgcgcc gcacaggggg gcaggcgacg
      661 cgcaccgttg tagcgattgt tctcgccgaa aactccgaag tcaaacatcc cgcggggcgt
      721 ggtgcgcctg gcgtgacgcg aacgcgcggg attgaacgtc gtggagacgg aagcgagtag
      781 gccccccccc cgagcctggc ccctgccgtg ccctgggcgt aggttggggg cgttcggacg
      841 cctcacgcga agggcggggt catgggcaag ttctttggaa gtgccggcgg ctggttcatc
      901 atccttctcg tcgcgctcgt cgcgctcgtc tcgggcatcg cgttcgggtg gttcgattcc
      961 ggaggctcac cgggcccagg cccatcgggc accgcgacgg tgaagtcgac cccgacatcg
     1021 agtaccacag cgacccccac ctcctttgtc gcccgccccg tcactacggg gccgcacgtt
     1081 ccctccgcgt ccgccctgac ggccgacgtc ctcgccgact cgggaagcgg gtgggtcgtc
     1141 gcgatcgacg acacgtccca ggtcaacacc ctcaacgacc caaacatcgt gatgccaggc
     1201 cagaagatcc tctacctcat ctcccccgac gggacccggt acgagctcgc gaacctcgac
     1261 gggatcggcc tcaccgaccc gaacctcgtg gcgtgggact acgcgcgcga tgtcctcctc
     1321 atggagcagg ccggcacgac gatcggcgtg ttcgacatgg agtccggcgc gatgacgtcg
     1381 tcctggacgt actgccccag cggccacgga agcgcgcggt acggcgaggc ccgcggcggg
     1441 aactgggtgg tccgcgctgg ttgcgagggc acgggagtgg acgccctcta caccgatgcc
     1501 ggggtcgtcg tgccctccac catccaggtc gcgccgccga tggtcaccaa cttcgacatc
     1561 gggaccatgc aggtgcggta cgagttcgag ggaaccgtcg acaagaagta ccttgcgtac
     1621 tcccccgatg gcgcctccga gcccctcccg tggagcgcgc tctcgtactg cgacaccccg
     1681 ctcggcaagg gccgcgagga caccctcgcc gtgctctgct attcggacgc gggcaagatt
     1741 tcgatcgagg agctcccagt gaacggctcc ccgccctacg aggtcgtttc gaccgcgcaa
     1801 ctcgacggct tcgccatggc gcacggcggt ttcggtcccg gcgagtacgg cctcacgggc
     1861 tactggtcgg acagcgccct cgggatcgtc cagttctggt tcggggacgc caactcgcgg
     1921 ctcggcgtcc tcaagggggg cacggtcgcg gccgtgggag cggacgcgtc ctaccccttc
     1981 cgccgagtcc acgccgtctc
//
LOCUS       DASVKP010000003         2699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1151235, whole genome shotgun sequence.
ACCESSION   DASVKP010000003 DASVKP010000000
VERSION     DASVKP010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151235"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..907)
                     /locus_tag="VF362_00070"
     CDS             complement(188..907)
                     /locus_tag="VF362_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7589358.1"
                     /translation="MSTLGPPPTRHAQPDLAVMGRADLARYLSAHSNLPGPRGNLELA
                     DAFVAAAPRETILAFADDEDEYLRFCGTQGLGRLLVESPGEAAVLATLVERAADPLWR
                     VREASARALQIVGRAAPGAMRAIVAQWMTHPDAYVRRAAVAAICEPALLRDPAGLRSG
                     LDACEAGTASLVALSPAERKEPAPRNLRQGLGYGWSVVVAASPTEGMPAFDRLRASDD
                     PDVAWIVASNLAKNRLKRLLS"
     gene            1237..2283
                     /locus_tag="VF362_00075"
     CDS             1237..2283
                     /locus_tag="VF362_00075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7589359.1"
                     /translation="MVLPSDDAFIVRVMDESSPTVPRGKRGLPISQMRNGDPGSITAA
                     IRMLNTADVAVVQHEYGIYGGPDGDEVLAILSRLDVPTVVVLHTVLAQPTPGQKRVLE
                     EVVRLASAVVVMTETARGNLQSRYLVDASAVRVIPHGVHPLENMAPLPHPDRPIVLTW
                     GLLGPGKGIEWAIQAFGKLQDLAPRPHYRVLGQTHPKVLANDGDVYRDRLVATVDALG
                     LGASITIDGRYLDPAALARNILVADIVLLPYDSRIQVTSGVLAEAVAAGKPVVATAFP
                     HAKELLASGAGIIVPHEDPAAIAAALRSLLTDADAAARMARIARKVGRESTWPEVGQR
                     FRTLALRLRSTVAA"
     gene            2334..>2699
                     /locus_tag="VF362_00080"
     CDS             2334..>2699
                     /locus_tag="VF362_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7589360.1"
                     /translation="MTDDRGLFEHALLDAPRAEFGYCVDDVARALLVTAREPQPGPRV
                     EEMTETYLAFVEAAIARDGRVRNRMNTAGVWTDVPATGDWWGRALWALGVAAVRAERP
                     ATRARALKAFERAARQESHD"
BASE COUNT          406 a          999 c          909 g          385 t
ORIGIN      
        1 catcagtttg gccgtgtccg ggcccgagaa gctgacgcta ccgtccggtc cgagcgtcac
       61 ctgcgtcgcg ggcgcatcgc ccaacttctt cccgaaccat cgcatgctcg atcgcacccc
      121 ttccgcggcc accgccccgt cgcggccggc cctgacgcaa gtgtaggaag cgcggagccc
      181 gggcgcgcta cgagaggagc cgcttgagcc ggttctttgc gaggttggaa gcgacgatcc
      241 atgcgacgtc cgggtcgtcg ctcgcccgga gacggtcgaa cgcgggcatc ccttccgtcg
      301 gactggcggc gacgacgacg ctccagccgt agccgagccc ctgccgaaga tttcggggcg
      361 cgggctcttt gcgttcggcg ggggagaggg cgacgagcga ggccgtgccc gcctcgcacg
      421 cgtcgaggcc cgagcggagg cccgcggggt ccctgaggag ggcgggctcg cagatcgcgg
      481 caaccgccgc gcggcgcacg taggcgtccg gatgcgtcat ccactgcgcg acgatggcac
      541 gcatggcgcc cggcgcggcg cggcccacga tctgaagcgc ccgcgcgctc gcctcgcgca
      601 cccgccacag cgggtcggcc gcgcgttcca cgagcgtcgc gagcaccgcc gcctcgcccg
      661 gcgactccac caggagcctc ccgagcccct gggtgccgca gaagcggagg tactcatcct
      721 cgtcgtcggc gaacgccaga atcgtctccc gcggcgcggc ggccacgaac gcgtcggcaa
      781 gctcgaggtt gccccggggg ccggggaggt tggagtgggc gctcaggtat cgcgcgagat
      841 ccgcccggcc catgacggcc aggtcgggct gcgcgtgccg cgttggaggg ggaccgaggg
      901 tgctcatgcc tacgacgcta ggggaggcgc gcgcgtttcg cacgccgtgc gcgattccac
      961 ccccaagaat ccgtccccgg cgcgtttcac caaatcgggg tatgctcaga gcaaagttcc
     1021 ttggtcgaga cactgccacc agggcgcgag ccggatagcg acaaaggagc cgctatcccc
     1081 tgaggggtgc ctcctggcag acgtctcacg tacgggaagc agcattgagt ttcccgatgg
     1141 agagaaggtc gcaccgtggc acgctttgga ttcctcagta cgtacccccc gacgcgttgc
     1201 ggcctcgcaa cgttcaccga gtcgcttgct ggctccatgg tccttcccag cgatgacgcg
     1261 ttcatcgtcc gggtcatgga cgagtcctca ccgaccgtcc cacgcggcaa gcgcgggttg
     1321 cccatctcgc agatgcgcaa cggggaccct gggtcgatta ccgccgccat ccggatgctc
     1381 aataccgccg acgtcgcggt cgtccagcac gaatacggca tctacggcgg accggacggc
     1441 gacgaggttc tcgccatcct ctccaggctc gacgttccga cggtggtggt gcttcacacc
     1501 gtgctcgcgc aaccaacgcc gggacagaag cgcgtgctcg aagaggtcgt gcgcctcgca
     1561 agcgccgtcg tggtcatgac ggagaccgcc cgcggtaacc tgcagagtcg ctatcttgtc
     1621 gatgcctccg ccgtccgcgt gatcccgcac ggcgtccacc cgctcgaaaa catggcgccc
     1681 ctgccgcacc ccgatcgccc tatcgtgctc acctggggcc tcctcgggcc gggcaagggc
     1741 atcgaatggg caatccaggc cttcggcaag ctccaggacc tcgcgccacg cccgcactac
     1801 cgcgtgctcg gccagaccca ccccaaggtc ctcgcgaacg acggcgacgt ctaccgcgac
     1861 cgcctagtcg ccaccgtcga cgccctcggc ctgggcgcga gcatcacgat cgacggccgc
     1921 tacctcgacc cggcggccct cgcccgcaac attctggtcg cggacatcgt gctactgccg
     1981 tacgactcgc gcatccaagt cacgtccggc gtcctcgccg aagccgtcgc cgcaggcaag
     2041 ccggtcgtcg ccacggcctt cccacacgcc aaggaactcc tcgcgagcgg cgcgggcatc
     2101 atcgtccccc acgaggaccc cgccgcgatc gccgcggctc tccggtccct cctcacggac
     2161 gccgacgccg ctgcgcggat ggcgcggatc gcgcgcaagg tggggcggga gtcgacgtgg
     2221 cccgaggtgg gccaacggtt ccgcacgctg gcgctacggt tgcgctccac tgtggcggcc
     2281 tgatgggtcc ccgactgagg agtcgcccag ccccgttcac ccatcttgat cgcatgacgg
     2341 acgaccgcgg cctcttcgag cacgccctcc tggacgcccc gcgcgccgag ttcgggtatt
     2401 gcgtcgacga cgtcgccagg gccctcctcg tcaccgcaag ggagccgcaa cccggccccc
     2461 gcgtcgagga gatgacggag acgtacctcg cgttcgtcga ggccgccatc gcgagggacg
     2521 gccgcgtccg caaccgaatg aacacggcgg gcgtgtggac ggacgttcct gccacggggg
     2581 actggtgggg acgggcgctc tgggcgctcg gcgtcgcggc cgtccgcgcg gagcggccag
     2641 ccacccgcgc gcgcgcgctc aaggccttcg agcgcgcggc aaggcaggag tcgcacgac
//
LOCUS       DASVKP010000004         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_987758, whole genome shotgun sequence.
ACCESSION   DASVKP010000004 DASVKP010000000
VERSION     DASVKP010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987758"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VF362_00085"
     CDS             <1..241
                     /locus_tag="VF362_00085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7589361.1"
                     /translation="LAETLGSLAAMVHAGPDGIAVGVDLNITHHRAARAGAVTGVATQ
                     IYAGRTVTTYGIEITGEDGKPVSTARLTCAIRPGA"
     gene            complement(242..871)
                     /locus_tag="VF362_00090"
     CDS             complement(242..871)
                     /locus_tag="VF362_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7589362.1"
                     /translation="MESPAPEGAVPPVPEPVGRRAVVAEDEALIRMDIVETLQEGGYD
                     VVGEAANGEEAIAVARALKPDVVVMDIKMPVMDGITAAETIVRERLAPVVLLTAFSQT
                     ELVERARDAGAMAYVVKPFTPADLLPAVEIARSRHAEMRALELEIADISERMETRKRV
                     ERAKGLLMENMQLNEPEAFRWIQKTSMDRRLTMREVADAVLGQMGPKKA"
     gene            905..978
                     /locus_tag="VF362_00095"
     tRNA            905..978
                     /locus_tag="VF362_00095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:939..941,aa:Leu,seq:caa)
     gene            1041..1496
                     /locus_tag="VF362_00100"
     CDS             1041..1496
                     /locus_tag="VF362_00100"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEX7589363.1"
                     /translation="MDGRIHNIALVAHDNKKAELLEWASFNKGSLAPHGLYATGTTGT
                     MLEYELGLHVHRFMSGPVGGDQQIGSKIAEGKIDMLIFFWDPLEPQPHDPDVKALLRI
                     STVWNVPMACNVATADFIISSPLFTEGYERVIPDHGPRDWEEMRSDDPT"
     gene            complement(1612..>1821)
                     /locus_tag="VF362_00105"
     CDS             complement(1612..>1821)
                     /locus_tag="VF362_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX7589364.1"
                     /translation="VGRLMGIAVTEGRAVRPGLEVGICGEHGGDPKSIAICHKLGLNY
                     VSCSPFRVPIARLAAAQAALADLKK"
BASE COUNT          311 a          629 c          602 g          279 t
ORIGIN      
        1 gctcgccgag accctgggga gcctcgcggc gatggtacac gcgggcccgg acgggatcgc
       61 cgtgggcgtt gatctgaaca tcacgcacca ccgcgccgcg cgtgcggggg cggtgacggg
      121 cgtcgcgacg cagatctacg cgggtcggac ggtcaccacc tacgggatcg agatcaccgg
      181 ggaggacggg aagccggtct cgaccgcgcg gctgacctgc gcgattcgcc cgggggccta
      241 gttaggcctt cttggggccc atctggccaa gcaccgcgtc ggcgacctcc cgcatggtca
      301 ggcggcgatc catggaggtc ttctgaatcc agcggaaggc ctctggctcg tttagttgca
      361 tgttctccat gaggaggccc ttggcgcgct ccacgcgctt gcgcgtctcc attcgctcgg
      421 agatatccgc gatctcgagt tcgagggcgc gcatctccgc gtggcgggat cgcgcgattt
      481 cgaccgcggg cagaaggtcg gccggggtga acggcttgac gacgtaggcc atcgcgccag
      541 cgtcgcgcgc acgctccacg agttccgtct gcgagaacgc cgtcagcagc acgacgggcg
      601 caagcctctc cctcacgatg gtttcggcgg cggtgatccc gtccatcacg ggcatcttga
      661 tatccatgac gacgacgtcc ggcttcaggg ccctcgcgac cgcgatcgcc tcctcgccgt
      721 tcgcggcctc gcccacaacg tcgtagccgc cctcctggag cgtctcgacg atgtccatgc
      781 gaatgagcgc ctcgtcctcg gccactacgg cgcgccgacc gacgggctcg ggcacgggtg
      841 ggacagcacc ttccggcgcg ggcgattcca cgggttcggt cacagccgtt agcctagtgc
      901 gtgcgccccg atagcccaac cggcagaggc atacggctca aacccgtacc agtgtgggtt
      961 cgaatcccac tcggggcacc gcagcgcgag cgacccgcgg gggcaacccc acccgaagcg
     1021 ggaaacctag ggaggtcggc atggacggaa gaatccacaa catcgcgctc gtggcgcacg
     1081 acaacaagaa ggccgagctc cttgagtggg ccagcttcaa caagggctcg cttgcgcccc
     1141 acgggctgta cgcgaccggc accacgggga cgatgctcga gtacgaactc ggcctgcacg
     1201 tccaccgctt catgtcgggc ccggtgggcg gcgatcaaca gatcgggtcc aagatcgccg
     1261 agggcaagat cgacatgctc atcttcttct gggacccgct ggagccgcag cctcacgatc
     1321 cggacgtgaa ggcactgcta cgcatcagta ccgtgtggaa cgtgccgatg gcgtgcaacg
     1381 tcgcgacggc ggatttcatc atctcctctc ccctgttcac cgagggctac gagagggtca
     1441 tcccggacca cggcccccgt gactgggagg agatgcgctc ggacgacccg acctaggggt
     1501 aagcgcccgc ccggcggcgc gaacggcccg ccccgagcgc cctagacccc tcgacgaaga
     1561 cacggccccc ggcgcaagcg ccgggggccg tgtgcgtgtg tacgaagcgt tctatttctt
     1621 gagatcggcc aaagcagcct gggccgccgc caaacgcgcg atcggcaccc ggaagggcga
     1681 gcagctcacg tagttcaggc cgagcttgtg gcagatcgca atcgacttgg ggtctccacc
     1741 gtgctcccca cagataccga cctcgagtcc cgggcggacc gcacggccct cggtaaccgc
     1801 gatgcccatg agcctgccaa c
//
LOCUS       DASVKP010000005         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_741446, whole genome shotgun sequence.
ACCESSION   DASVKP010000005 DASVKP010000000
VERSION     DASVKP010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741446"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1722
                     /locus_tag="VF362_00110"
     CDS             34..1722
                     /locus_tag="VF362_00110"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEX7589365.1"
                     /translation="MTIPTVNDWRRRDRTRQMAAIAVAAVAVVGILSATSAPLRGRFK
                     VVLEIVPFHATRGAATTLVLASFALLLTARGLRRGGRLAWAGTLALLVLSVGLNLAKG
                     LDIEEAVLALGAGAWLATRRRAFPVLPTRAAARMAAILGVGGAAGALVFGVALSMAVG
                     RRHHPHLGESTRAVAERLGGNWALPLPGAGPFVTPMLVAVGIGLVGSTLWLLLSPRPG
                     RRLSAPAHLAERERARRVVAAHGGGTLDYFALRDDKDWFFYGESVVAHSVRRGVCLVS
                     PDPIGPDAERAAALAAFLAFTQREGWSVAVIGASAAWLPLYQAAGMRPLYMGDEAVVD
                     CGPFTLEGPPMKSLRGAYARVERAGYTADFIDPADLTAEDRAALEALTEASRHGEAER
                     GFSMTLSRLCDPHDEGLLLTVVRDAERTVQAFVQWMPAAKIGGWSLDVMRRNPSHDLP
                     NGVMDFAIVATILHLAPTGGGLGLNFAMFREVVASESPSGMARLSKAALRAFSGKAQV
                     ESLWRFNSKYNPDWIPRYVVLDSLDLAVTQGFVMADAEGVTELPLIGRFLGGTP"
     gene            1719..>2493
                     /locus_tag="VF362_00115"
     CDS             1719..>2493
                     /locus_tag="VF362_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589366.1"
                     /translation="MTLSLIAAVAGAVLYGVGSVLQAAAVRRSGGLRALRQPLYLAGL
                     ACDGAAWLASLVALHALPLFTVQAVLAGSLAVAVVLARLVLRAVLRPVDGVAVGVVVA
                     ALVVLALAAGPERGAHGGVAVVPAWFAAATLVALAVLVAATALTYRRGAGLWFAVLAG
                     LASSGAAIAVRATHVEARVSALALADVARQPLAWAVIGFGALTAVTYARALERAAVGP
                     ATAVLSVIDVVVPGAVGLAVLHDVIRSGWAFGAAAATLVA"
BASE COUNT          263 a          862 c          931 g          437 t
ORIGIN      
        1 gcggttctac tccgtctgac acacaatggc cccatgacta ttcccaccgt caacgattgg
       61 cggcgccgag atcgcaccag gcagatggcc gcgattgcgg tcgcggctgt ggcggtggtg
      121 gggatcctct ccgcgacctc cgcgccgctc aggggccgat tcaaagtggt cctcgagatc
      181 gtgccctttc acgcgacgcg gggagcggcc accacgctcg tcctcgcgtc gttcgcgcta
      241 ttgctcaccg cgaggggcct caggcgcgga ggcaggctcg catgggcggg aaccctcgcc
      301 ttgctcgtgc tgtcggtggg actgaacctc gccaagggcc tcgacatcga ggaggcggtg
      361 ctcgctctcg gcgcaggagc atggcttgcc acgaggaggc gcgccttccc cgtgttgccg
      421 acacgcgcgg ccgcgcgcat ggcggccatt ctcggcgtgg gcggggcggc cggggcgctc
      481 gtgttcggcg tggcgctgtc gatggccgtt gggcgacggc atcaccctca ccttggcgag
      541 tcgacgcgtg ccgtcgccga gcgcttgggc ggcaactggg cgttgccgct tcccggggct
      601 ggccccttcg tcacgccgat gctcgtggcc gtcggcatcg gactggtggg ttcgacgctg
      661 tggctgctgt tgtcgccacg gcctgggcgg cgcctgtctg cgcccgcgca cctggccgag
      721 cgcgagaggg cccgccgggt ggtcgccgcg catggaggcg gcaccctcga ctacttcgcg
      781 ctgcgtgatg acaaggactg gttcttctac ggcgagtccg tggtcgcgca ttcggtgcgc
      841 cgcggcgtct gcctggtgtc tcccgacccg atcggacccg acgcggaaag ggccgccgcg
      901 cttgccgcct tcctcgcctt cacccagcgc gagggatggt cggttgccgt gatcggcgcg
      961 tcggccgcgt ggttgccgct gtaccaagcc gccggaatgc gcccgctcta catgggcgac
     1021 gaggccgtgg tggactgcgg gcccttcacg ctcgagggcc cccccatgaa gagcctgcgc
     1081 ggcgcctacg cgcgggtgga gcgcgcgggc tacaccgccg acttcatcga cccggccgac
     1141 ctgacggccg aggacagggc cgcgcttgag gcgctcaccg aggcaagccg ccatggcgag
     1201 gcggaaaggg gcttctcgat gacgctgtcg cgcctgtgcg acccgcacga tgagggcctc
     1261 ctcctcaccg tggtgcgcga cgcggagcga accgtccagg ccttcgtgca atggatgccc
     1321 gcggcgaaga tcggcgggtg gtccctcgac gtgatgcggc gcaacccgtc ccatgatctg
     1381 cccaacggcg tcatggactt cgcgatcgtt gccacgatcc tccacctcgc gccgacgggt
     1441 ggaggtctcg gcctgaactt cgcgatgttc agggaggtgg tcgcgagcga gtctccctcg
     1501 gggatggcga ggctctccaa ggccgcactg agggccttct cgggcaaggc gcaggtcgag
     1561 tcgctctggc gcttcaactc gaagtacaac cccgactgga ttccgcgcta cgtggtgctc
     1621 gactccctgg acctcgcggt cacccaaggt ttcgtcatgg ccgacgcgga gggcgttacc
     1681 gagttgcccc tcatcgggcg gttccttggc gggaccccgt gacgctgtcg ctcatcgcgg
     1741 ccgttgcggg cgcggtgctc tatggcgtgg gctcggtgct ccaggcggcg gcggtgcggc
     1801 gatcgggggg cctccgcgcc ttgaggcagc cgctctacct cgcgggtctc gcgtgcgatg
     1861 gtgcggcttg gctcgcgtcg ctggtggccc tgcacgcgtt gccgctcttc accgtgcagg
     1921 cggtgctcgc gggctccctc gccgtggcgg tggtgctcgc gcggctcgtg ttgcgcgcgg
     1981 tgcttcgccc cgtcgatggg gtggccgttg gtgtggtggt agcggcgctc gtggtgctcg
     2041 cgctcgccgc ggggccggag cgcggggcgc acggcggggt cgcggtcgtc cccgcgtggt
     2101 tcgccgccgc gacgctcgtg gcgctggccg ttctcgttgc cgcgacggcg cttacctatc
     2161 gtcggggtgc cggcctgtgg ttcgcggtac ttgccggcct cgcgtcctct ggcgcggcca
     2221 tcgccgtccg cgccacgcac gtggaggcac gagtgagcgc gctggccctc gccgatgtgg
     2281 cacggcagcc gctcgcgtgg gcggtcatcg gcttcggggc gctcaccgcg gtgacgtacg
     2341 cgagggcgct cgaacgggcc gccgtgggtc cggccaccgc ggtgctgtcg gtgatcgacg
     2401 tggtggtccc cggcgcggtg gggctcgccg tgctccacga cgttatccgc tccggctggg
     2461 cgttcggcgc cgcggccgcc accctggtgg ccc
//
LOCUS       DASVKP010000006         5536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_823857, whole genome shotgun sequence.
ACCESSION   DASVKP010000006 DASVKP010000000
VERSION     DASVKP010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823857"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VF362_00120"
     CDS             <1..542
                     /locus_tag="VF362_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX7589367.1"
                     /translation="RVTHWLSPDCGLDQCWQAIHGTWALASGGIWGLGPGLSREKWSY
                     LPKAHNDFIFAILGEEFGLVGTIFVLIALAMIAIAVNRIVQRHNDPFIQIAAAAIGAW
                     IVGQACLNIAVVLGLAPVTGVPLPLVSSGGSSMIMTLVGIGVLLAFARREPGAAEAFA
                     VRPSLVRRSLAVVSGRRRG"
     gene            535..1653
                     /gene="murG"
                     /locus_tag="VF362_00125"
     CDS             535..1653
                     /gene="murG"
                     /locus_tag="VF362_00125"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX7589368.1"
                     /translation="MGKTILLAGGGTAGHVSPLLATAAELARRGHHVVALGTAAGLEA
                     DLVPRAGVAFRAIPRVPFPRRVNAAAARFPGAMVGALRATGRVIDEVAPDVVVGFGGF
                     VSTPAYLVAHRRGIPIVVHEANSKAGLANRLGARYAAGVATNYASTELRGAVVIGMPL
                     REEIVALAEALRDPRSATVARIKARLALRWPDDAPAVLVMGGSLGASRLNSATAEAIP
                     AIVGHGIHVLHITGRGKDDSPARALGDLAAKFRTQYVVREYEHDIARAYAAVDAVVCR
                     AGAVTVAEVSALGLPALYVPLPIGNGEQALNAAPAVAAGAALVIENEWLTAAAVTLAL
                     EEILLDVDDRVRITEAARSLGVLDGSARLADMIERVAT"
     gene            1650..3011
                     /gene="murC"
                     /locus_tag="VF362_00130"
     CDS             1650..3011
                     /gene="murC"
                     /locus_tag="VF362_00130"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX7589369.1"
                     /translation="MTEGRVHFIGVGGAGMSAVAWLLACAGVPVSGSDAAEGPYLAAL
                     RDAGLDVRVGHDPALVAGASVVVVTSAVRESNPELIAARQSGIPVWHRSEALVRAVAG
                     KRVIAVAGAHGKTTTAAMTAHALRECGIDASFAIGAPVLGVSGAVGGAYAGASDVAVI
                     EADESDGSFLAYHPQIAIITNVEADHLDHYGTVEAVEEAFRQFAATAGMVIACGDDAP
                     AALVAADAAGRGVSVETYGTGGADVTVDATSVGRGAERFAMEVSQPGAHNRLNATAAW
                     AAAVAVGADPLGAAAAMARFPGTGRRYELRGKAGGVRVVDDYAHHPTEIAALLAAARE
                     REPGRLVVLFQPHLYSRTRLLAPAFARALDEPGAHVIVSGIYGAREDPEPGVGPRTIT
                     DLMGATRAASVEAIEDLRAGAERAAELAEPGDTILMVGAGSVTDIASRILDRLRTRAR
                     DRG"
     gene            3004..3795
                     /locus_tag="VF362_00135"
     CDS             3004..3795
                     /locus_tag="VF362_00135"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX7589370.1"
                     /translation="MAERAGTAVATPPQESPAGSERRRRGAERAGARARARRRRTARL
                     ALTISTVAIAVWAGWFSPLFALDASKVELAGLGESVDGGAVRAALGPYVGTPLPRLDP
                     GAMAVAVEGIDGVRSAVVTRKWPDGVRITVTPRVAIAAVPGKDGAYVFVDAEGVALGQ
                     SAADPGELPVVDVVVGPDHARALDAVVSVLGGLPTELGQRVRSAGAQTEDTVELTLGD
                     GTKVVWGSASDTALKAQVVLTMLDSAAFAGVVIDVSAPTLPVTHG"
     gene            3927..5093
                     /gene="ftsZ"
                     /locus_tag="VF362_00140"
     CDS             3927..5093
                     /gene="ftsZ"
                     /locus_tag="VF362_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX7589371.1"
                     /translation="MASPQNYITAIKVVGVGGGGVNAVNRMIDVGLKGAEFIAINTDA
                     QALLMSDADVKLDIGREMTRGLGAGADPEVGRRATEDHADEIEEALRGADMVFVTAGE
                     GGGTGTGGAPVVARIARALGALTVGVVTKPFSFEGRRRSDQADAGVELLRDQVDTLIV
                     IPNDRLLDISDKHLSVLGAFAAADQVLQGGVQGITDLITTPGLINVDFNDVRSVMQGA
                     GTALMGVSSARGEDRATKAAEAAISSPLLEASIEGAHGVLLSIAGGSNLGIKEVETAA
                     KLVREAAHAEANIIFGTVIDDALGDEIRITVIAAGFDGGAPPVRRHPGALAQVDGAQP
                     KEAKTKEPVDALLEIPPRSRASKAEPVQSEAEALKVAGGLFESEELDIPDFLKR"
     gene            5090..>5536
                     /locus_tag="VF362_00145"
     CDS             5090..>5536
                     /locus_tag="VF362_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HEX7589372.1"
                     /translation="MTSPYAPVPTLDAGLPVGAFFTTRDGGFSAAPFDSLNAAGHVGD
                     VQEAVDANRTRIDGLARARVVYMNQVHGAVVARVHRADEHVEADGLITDAPGLALAVL
                     VADCVPVLLHDASTGAVAAVHAGRQGIAKGVVVRAVEALGALHSRGG"
BASE COUNT          757 a         1877 c         2069 g          833 t
ORIGIN      
        1 tgcgcgtcac ccactggctg tcccccgatt gcgggctcga ccagtgctgg caggcgatcc
       61 atggaacatg ggccctcgcc tcggggggaa tctgggggct cgggccggga ctctcgcgcg
      121 agaagtggtc ctacctcccc aaggcccaca acgacttcat cttcgcgatc ctcggagagg
      181 agttcggcct ggtggggacg atcttcgtcc tcatcgcgct cgccatgatc gcgatcgcgg
      241 tcaaccgcat cgtccaacgc cacaatgatc cgttcattca gatcgcggcc gccgcgatcg
      301 gcgcgtggat cgttgggcag gcgtgcctca atatcgcggt cgtgctcggg ctcgctccgg
      361 tgacgggcgt gccgcttccg ctcgtgtcgt ctgggggatc atcgatgatc atgacgctcg
      421 tggggatcgg agtgctcctc gctttcgcgc gtcgtgagcc cggtgccgca gaggcgttcg
      481 cggtgcgccc gtcgctcgtg cggcgctctc tggcggtcgt ctcggggagg cgccgtgggt
      541 aagacgatcc tcctggcggg gggaggcacc gcgggacacg tcagcccgct cctcgcgacc
      601 gccgccgagc ttgcccgccg gggccaccat gtcgtggcgc tcgggaccgc ggcggggctc
      661 gaggccgacc tggtgccgcg cgcgggcgtc gccttccgcg cgatcccccg cgtgccgttc
      721 ccgcgcagag tcaacgccgc ggccgcacgg ttccccgggg ccatggtggg ggccctgcgc
      781 gcgaccggtc gtgtcatcga cgaggtcgcc ccggacgtcg tcgtcgggtt cggcggattc
      841 gtttcgaccc ccgcctacct ggttgcccac cgacgcggga ttccgatcgt ggtccacgag
      901 gcgaactcca aggcgggtct cgcgaatcgc ctcggcgcgc gctatgcggc gggggtggcg
      961 acgaactacg cctcgaccga actgaggggg gcggtcgtca tcgggatgcc gcttcgggag
     1021 gagatcgttg ccctggccga agcgctgcgg gacccccgct cggcaaccgt cgcccggatc
     1081 aaggcgaggc tcgcccttcg gtggcccgac gacgcgcccg cggtcctggt gatgggcggc
     1141 tccctaggtg cctcccgcct caattccgcg accgcggagg ccatccctgc gatcgtcggg
     1201 cacgggattc acgttcttca catcacggga cgcggcaagg acgacagccc cgctcgcgcc
     1261 ctcggcgacc tcgcggcgaa gttcaggact cagtacgtgg tgagggagta cgagcacgac
     1321 atcgcccgcg cgtacgcggc ggttgacgcc gtcgtgtgtc gcgcgggcgc cgtcaccgtt
     1381 gcggaggtct ccgccctcgg gcttcccgcc ctctatgttc cgctcccgat tgggaacgga
     1441 gagcaggcgc tcaatgccgc gcccgccgtc gccgccggag cggcattggt catcgagaac
     1501 gagtggctca ccgcggcggc cgtgacgctc gcgctcgagg agatcctcct cgacgtggac
     1561 gatcgcgtcc ggatcaccga ggccgcgcga tcgctgggcg tcctcgacgg tagcgcgcgc
     1621 cttgccgaca tgatcgagcg ggtggcgacg tgaccgaggg ccgcgtgcac ttcatcgggg
     1681 tgggcggggc gggaatgtcg gccgtcgcgt ggttgctggc gtgcgcgggg gtgcccgtct
     1741 ccggtagcga cgccgcggaa ggcccgtacc tggccgccct gcgcgacgcc ggcctggacg
     1801 ttcgcgtcgg acacgatccg gcgctcgtcg cgggcgcctc cgtcgtcgtc gtgacgtccg
     1861 cggtgcggga gtcgaacccc gagctgatcg ccgcgcgcca atccggtatc ccggtctggc
     1921 accgctcgga ggcgctggtg cgcgccgtcg caggaaagcg cgtgatcgcc gtcgccgggg
     1981 cgcacggcaa gacgacgacg gcggcgatga cggctcacgc gctgagggag tgcgggattg
     2041 acgcaagttt cgccatcggc gcaccggtgc tcggggtgtc cggcgcggtc gggggcgcat
     2101 acgcgggcgc gagcgacgtg gcggtcatcg aggcggacga gtcggacggg tccttcctcg
     2161 cgtaccaccc ccagatcgcg atcatcacga acgtcgaggc ggatcacctg gaccactacg
     2221 ggacggtcga ggcggtggag gaggccttcc ggcagtttgc cgccaccgcg ggcatggtta
     2281 tcgcctgcgg tgacgacgcg cccgccgccc tcgttgccgc cgacgcggcg ggaaggggtg
     2341 tctccgtcga gacgtatggg accggcgggg ccgacgtgac ggtggacgcg acctccgtgg
     2401 gccgcggcgc agagcggttc gcgatggagg tttcgcagcc gggggcccac aatcgcctca
     2461 acgccacggc ggcgtgggcg gcggccgtgg cggtcggcgc agacccgcta ggggcggcgg
     2521 cggcgatggc gaggttcccc ggcaccggcc ggcgatacga actccgcggc aaggcgggcg
     2581 gggtccgcgt ggtcgatgac tacgcccatc accccacgga gattgcggcg ctgctcgccg
     2641 ccgctcggga gcgtgaaccc gggcgcctcg tcgtgctgtt ccagccgcac ctgtactcgc
     2701 ggacgcggct cttggcgccg gcgtttgcgc gggccctgga cgagcctggg gcccacgtga
     2761 tcgtctccgg catctacggg gcccgggaag accccgagcc gggggtgggg ccgcgcacca
     2821 tcacggacct catgggcgcc acgagggcgg cctcggtcga ggcgatcgag gacctgcgcg
     2881 ctggcgccga gcgcgccgcg gagctggcgg agccggggga cacgatcctc atggtgggcg
     2941 cgggttcggt cacggacatc gcgtcgcgca ttctcgatcg ccttcgcacg agggcgcgtg
     3001 atcgtggctg aacgagctgg cactgcggtg gcgacgccgc cgcaggagtc tcccgctggc
     3061 tccgagcgcc gccgaagggg cgcggagcgg gcgggcgcgc gcgccagggc gcgtcgccgc
     3121 cgcacggcgc ggctcgccct cacgatctcg acggtcgcga tcgcagtatg ggcaggctgg
     3181 ttctcgccgc tgtttgccct cgacgcctcc aaggtggagc tcgcggggtt gggggagtcg
     3241 gtcgatggcg gggcggttcg agccgcgctc ggcccctatg tcggcacccc gcttccccgc
     3301 ctcgatcccg gagcgatggc cgtggccgtc gagggtatcg acggcgtgcg atccgcagtt
     3361 gtcacgagga agtggccgga cggtgtccgg attaccgtca cgccgagggt cgcgattgcg
     3421 gccgtccccg ggaaggacgg ggcctacgtc ttcgtcgacg cggagggtgt ggcgcttggc
     3481 cagtcggccg ccgaccccgg cgagctcccc gtcgtcgacg tggtcgtcgg cccggatcac
     3541 gcgagggcgc tggacgcggt ggtctcggtc ctcgggggcc taccgaccga actcggacag
     3601 cgcgtgcgtt ccgcgggggc gcagaccgag gacaccgttg agttgacgct cggcgatggc
     3661 accaaagtgg tgtgggggag cgcgagcgac acggcgctca aggcgcaggt tgtcctcacc
     3721 atgctcgact cggctgcgtt tgcaggcgtg gtcatcgacg tctccgcgcc gacccttccc
     3781 gtcacgcacg gctagcgcga caaatgcgcc cgacacgcgc ggcgatcacg gcgcggcgcg
     3841 gggcgcggca ttaccgtaac ggtggatcta cttgacataa ctctaactct caagttgaag
     3901 gttaaagttg aacgaaaggc cgggccgtgg cgtctccgca gaactacatc accgcgatca
     3961 aggtcgtggg cgtgggcggc ggcggcgtga acgccgtcaa ccggatgatc gacgtcgggt
     4021 tgaagggcgc ggagttcatc gcgattaaca ccgacgcgca ggcgctcctg atgtccgacg
     4081 cggacgtcaa gctcgacatc ggcagggaga tgacgagggg actcggggcc ggagccgacc
     4141 ccgaggtggg gcgcagggcc acggaggacc acgcggacga gatcgaggag gcgcttcggg
     4201 gcgccgacat ggtcttcgtg accgcgggcg agggcggagg caccggaacc gggggcgctc
     4261 ccgtcgtcgc gcggatcgcg cgggcgcttg gggcgctcac cgtcggcgtc gtcaccaagc
     4321 cgttcagttt cgagggccgc cgccgctccg accaggcaga cgccggggtg gagttgctcc
     4381 gcgaccaggt ggacacgctc atcgtgatcc ccaacgaccg ccttctcgac atctcggaca
     4441 agcacctgtc cgtcctcggc gcgttcgcgg ccgccgacca ggtgctccag ggcggcgtcc
     4501 agggcatcac ggatctcatc accacgcccg ggctgatcaa cgtggacttc aacgacgtgc
     4561 ggagcgtgat gcagggcgcg ggaacggcgc tgatgggggt gagctcggcg cgcggggagg
     4621 atcgcgcgac caaagcggcg gaggccgcga tctcctcgcc cctcctcgag gcgagcatcg
     4681 agggcgcgca tggagtcctc ctcagcatcg ctggcggatc caacctcggc atcaaggagg
     4741 tggagaccgc ggcgaagctc gtgcgcgagg ccgcccacgc cgaggccaac atcatcttcg
     4801 gcaccgtgat cgatgatgcg ctcggggacg agattcgcat tacggtcatc gccgcgggct
     4861 tcgacggagg cgcgccaccc gtgcgacgcc accccggcgc cctcgctcag gtggacggcg
     4921 cgcagccgaa ggaggcgaag acaaaggagc ccgtcgacgc actgctggag ataccccccc
     4981 gatcgcgggc atcgaaggcc gagcccgtcc agagcgaggc cgaggcgctt aaggtcgccg
     5041 gcggcctgtt cgagagcgaa gaactcgaca tcccggactt cctcaagcgg tgacctcgcc
     5101 gtacgcccca gtcccgacgc tcgacgcggg acttcccgtt ggagcgttct tcacgacgcg
     5161 ggacgggggt ttcagcgcgg cccccttcga ttcgctcaat gcggccgggc acgtgggcga
     5221 cgttcaggag gcggtggacg cgaatcggac gcggatcgat ggcctcgcgc gcgctcgcgt
     5281 cgtctacatg aatcaggttc acggggctgt cgtcgcccgc gtccatcgtg ccgacgagca
     5341 tgtggaggcg gatggcctca tcaccgatgc gcccggccta gccctcgccg tcctcgtcgc
     5401 cgactgcgtc cccgtgctcc tgcacgacgc ctcgaccgga gccgtggcgg ccgtccacgc
     5461 gggtaggcag ggcatcgcga agggtgtcgt cgtgcgtgcg gtggaggcgc tgggcgccct
     5521 gcattcgcgc ggggga
//
LOCUS       DASVKP010000007         3295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_413542, whole genome shotgun sequence.
ACCESSION   DASVKP010000007 DASVKP010000000
VERSION     DASVKP010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413542"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VF362_00150"
     CDS             <1..341
                     /locus_tag="VF362_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7589373.1"
                     /translation="ERALGERLRRGVEAVDGVRLLGPSGSSGENVLGLAAFVVDGVHP
                     HDVGQVLDDAGIAVRVGHHCAQPLHRRLGVPNSTRASTYVYTTEAEVDAFLASLSGVR
                     TFFGLPAVTA"
     gene            356..811
                     /locus_tag="VF362_00155"
     CDS             356..811
                     /locus_tag="VF362_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX7589374.1"
                     /translation="MYQQVIMDHAKEAHGRGLAPALGASVGESFQVNTTCGDEVRLRV
                     GIEGDRVASLTWEGQGCSISQASVSILHDLVVGATLDDTRAAEAAFLALMQARGEEPD
                     AATEDLLGDATAFVGVGRYPARIKCALLGWMALKDALIQANVPPERRDS"
     gene            808..1152
                     /locus_tag="VF362_00160"
     CDS             808..1152
                     /locus_tag="VF362_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX7589375.1"
                     /translation="MTESTPPTSDAAISNAADVEEAMRDVIDPELGINVVDLGLVYGV
                     ALDANRHATIDMTLTSAACPLTDVIEDQTAQALDGIVDGFRINWVWMPPWGPDKITDE
                     GREQLRALGFNV"
     gene            complement(1245..1889)
                     /locus_tag="VF362_00165"
     CDS             complement(1245..1889)
                     /locus_tag="VF362_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HEX7589376.1"
                     /translation="MNAPATSPTAATIPGVPFPLAPSAGARWTIEGDPARVTVLAGAH
                     TDIFVDPGGASQVNAETLLNAATLLGTPPDGDFRLSAHVRVAFASTFDAGVLLLWFDE
                     GHWAKLCLEYSPEGEPMVVSVVNRGVADDANSFVVPGESIWLRVSRVDSVYAYHASED
                     GERWRLVRAFVLDVPGASPRVGFEAQSPTGKGCAVSFDQIGFDRVRLGDLRDGT"
     gene            complement(1894..2808)
                     /locus_tag="VF362_00170"
     CDS             complement(1894..2808)
                     /locus_tag="VF362_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX7589377.1"
                     /translation="MTFTSGSRLDPSRVQRRGASAGAGGMAIGGGIGGLLLLLVGAYL
                     GVDLTGLFGGGTSGSSVSANDPAAQAQLEQKCTTGADANNSTDCLVVGTVNSLDSYWS
                     SAMPALGASFSYPEIVLFDSQTSTGCGTASVQTGPFYCPPDKTIYVETNFFQILAEFG
                     GSTGPLAQEYVIAHEYGHHIQQLLGVFDVADRTGTGANSDSVKVELMADCLAGVWTHY
                     AATAPDASGTPFLVPPTDAEINDALSAAGSVGDDNIQQASGGQANPDSFTHGTSAQRV
                     QAFTLGYQAGSVAQCDPFGVTGSGSGAN"
     gene            complement(2862..>3295)
                     /locus_tag="VF362_00175"
     CDS             complement(2862..>3295)
                     /locus_tag="VF362_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEX7589378.1"
                     /translation="VLVIAAAAGAYQLWQGDARLAVDLAADFVSLICLSLAVTTSTPL
                     DESMGLVSAAARPIRRWIPPEALALVFSLTIRAIPELTRILAESRDAARSRGLDRSPR
                     AMLIPAGVRTVGYALAVGDAITARGLADEALPVGGREACAR"
BASE COUNT          501 a         1117 c         1218 g          459 t
ORIGIN      
        1 acgagcgcgc cctcggcgag cggctccgcc gcggcgtcga ggccgtcgac ggggtgcgcc
       61 tgctcgggcc ttcgggctcg tcgggagaga acgtcctcgg cctcgcggcg ttcgtcgtgg
      121 acggcgtcca ccctcacgac gtcggccagg tcctcgacga cgcgggcatc gcggtgcgcg
      181 tcggccacca ctgcgcccag ccgcttcacc gccgcctcgg cgtcccgaac tcgactcgcg
      241 catcgaccta cgtctacacc accgaggccg aggtcgacgc gttcctcgcg tccctctcgg
      301 gcgtccggac gttcttcggc ctcccggcgg tgacggcgtg agcgcgctcg agcagatgta
      361 ccagcaggtg atcatggacc acgcgaagga ggcccacggt cgcggcctcg cgcccgcgct
      421 cggcgcgagc gtcggggagt cgtttcaggt caacacgacg tgcggcgatg aggtccgcct
      481 tcgcgtcggc atcgagggcg accgagtggc gagcctgacg tgggaagggc agggctgctc
      541 aatctcccag gcgagcgtgt ccatcctgca cgacctggtc gtcggggcca ccctcgacga
      601 cacccgcgcc gcggaggccg cgttccttgc actgatgcag gcccgcggcg aggagcccga
      661 cgcggcgacc gaggacctcc tgggcgacgc gacggccttc gtcggcgtcg gccggtaccc
      721 ggcgcgcatc aagtgcgcgc tgctgggatg gatggcgctc aaggatgcgt tgatacaggc
      781 gaacgtccca ccggaaaggc gagactcatg accgagagca ctccccctac ttcagacgcc
      841 gcgatcagca acgcggcgga cgtcgaggag gccatgcgcg acgtgatcga tcccgaactg
      901 gggatcaacg tcgtggacct cgggctcgta tacggggtcg ccctcgacgc caacaggcac
      961 gcgaccatcg acatgacgct gacaagcgcc gcgtgcccgc tcacggacgt gattgaggat
     1021 cagacggcgc aggccctcga cggcattgtc gacggcttcc gcatcaactg ggtctggatg
     1081 ccgccgtggg gtcccgacaa gatcaccgac gaggggcgcg aacagttgag ggccctcggc
     1141 ttcaacgtgt agccgcgcgc gggcgcccgg tgggccagga aacccttcgc gcaaggcgtg
     1201 ccgtgggcac gtgccgcccg cggatcgggc gcgctcccct tcggctaggt gccgtccctg
     1261 aggtcaccca ggcgcacccg gtcgaatccg atttgatcga agctgaccgc gcatcccttg
     1321 cccgtcgggg actgggcctc gaatccgacg cgaggcgacg ccccgggaac atccagaacg
     1381 aaggcgcgaa caagccgcca cctctcgccg tcttccgagg cgtggtaggc gtacacggag
     1441 tcgacgcggg agacgcggag ccagatggac tcgccgggga ccacgaacga gttcgcgtcg
     1501 tcggcgacgc cccggttcac gaccgacacg accatgggtt cgccctcggg ggagtactcg
     1561 aggcagagct tggcccagtg accctcgtcg aaccacagga ggagcacgcc cgcgtcgaag
     1621 gtcgaggcga acgccaccct cacgtgggcg ctgagccgga agtcgccgtc agggggcgtc
     1681 ccgagaagcg tcgccgcgtt gaggagcgtc tcggcgttga cctgggaggc gccgcccggg
     1741 tcgacgaaga tgtccgtgtg ggcgccggcg agcaccgtca cccgcgccgg atccccctcg
     1801 atcgtccagc gggcgcccgc agagggcgcg agcgggaacg gaacgccggg gatcgtcgcg
     1861 gcggtagggg acgttgctgg cgcgttcacg gggctagttt gcgccgctgc cggatccggt
     1921 gactccgaac gggtcgcact gggcgacgga gccggcctgg tatccgaggg tgaacgcctg
     1981 gacccgctgg gccgacgtcc cgtgggtgaa cgaatcgggg ttcgcctgcc cccccgaagc
     2041 ctgctggatg ttgtcgtcgc cgaccgagcc ggccgccgag agcgcgtcgt tgatctccgc
     2101 gtcggtcggc ggcacgagga acggcgttcc gctcgcgtcg ggcgccgtcg cggcgtagtg
     2161 cgtccatacg cccgcgaggc agtccgccat caactcgacc ttgaccgagt cggagttggc
     2221 gccggtgccg gtgcggtccg ccacgtcgaa gacgccgagg agttgctgga tgtggtgtcc
     2281 gtactcgtgg gcgatcacgt actcctgcgc gagggggccg gtcgagccgc cgaactcggc
     2341 gaggatctgg aagaagttcg tctcgacgta gatcgtcttg tcaggggggc agtagaacgg
     2401 gccggtctgg acggaggccg tgccgcagcc cgtcgacgtt tggctgtcga acagcacgat
     2461 ctcggggtag gagaagctcg cgccgagggc gggcatcgcc gaggaccaat aggagtcgag
     2521 cgagttcacc gtgccgacga cgaggcagtc ggtgctgttg ttcgcgtccg cgcccgtggt
     2581 gcacttctgc tccagttgcg cttgggcggc cggatcgttc gcggagaccg acgagcccga
     2641 ggtgccgccg ccgaagaggc ccgtgagatc cacacccagg taggcgccga cgaggaggag
     2701 gagcaggccc cctatgccgc cgccgatggc catgcccccc gcgcccgccg aggcgccccg
     2761 gcgctgtacg cggctggggt cgaggcggga gccgctggtg aacgtcatgc gaacctcctg
     2821 aggtgtacaa acgagggtac cgtgcggggg cgcgggcgtc tctagcgcgc gcaggcttcg
     2881 cgcccgccca ccggcagggc ctcgtcggcg agtccccgcg cggtgatcgc gtcgccgacc
     2941 gcgagggcgt atccgaccgt ccttaccccc gcgggaatga gcatcgcgcg cggcgaccgg
     3001 tcgaggccgc gggacctcgc ggcgtcgcgc gactccgcga ggatcctcgt caactccggg
     3061 atggcgcgga tcgtcaggct gaagaccagg gcgagggcct cggggggaat ccaccggcga
     3121 atcggtcggg ccgcggcgct cacgagcccc atcgattcgt cgagcggcgt cgaggtggtg
     3181 acggcgagcg agaggcagat gagggagacg aagtcggccg cgaggtcgac ggcgagcctc
     3241 gcgtcgccct gccacagttg gtaggcgccg gccgcggccg cgatcacgag cacgg
//
LOCUS       DASVKP010000008         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_413576, whole genome shotgun sequence.
ACCESSION   DASVKP010000008 DASVKP010000000
VERSION     DASVKP010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413576"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /gene="metE"
                     /locus_tag="VF362_00180"
     CDS             <1..1525
                     /gene="metE"
                     /locus_tag="VF362_00180"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEX7589379.1"
                     /translation="GSLGDALPVLVGTPVEAIGIDLVRGDVPVGVSLTGKVIAAGVVD
                     GHNIWRTDLDAALAKVAACEALGGTVTVSTSTSLLHVPHTTAGEEHLGEILPWLAFAD
                     EKVAEIATLAKARAQGESAVADALAAARAALASRAANAGTHVAAVREALAAVKPADYD
                     RASDYRTRDAAQAERFHLPALATTTIGSFPQTPEIRKVRAALVAGEITQAVYEDAMKG
                     EIASVIALQEDLGLDVLVHGEAERNDMVQYFAELLDGFAVTKNGWVQSYGSRCTRPSI
                     LWGDVSRPEPMTVKWATYAQSLSEKPVKGMLTGPVTILAWSFVRDDQPLGDTARQVGL
                     ALRHEIADLEAAGIGIIQVDEPALRELLPLKKRDQAAYLDWSVGSFRLATSGAADATQ
                     IHTHLCYSEFGEIIDAIDGLGADVTSIEAARSRMEVLPAIKAHGFERGIGPGVWDIHS
                     PRVPSTQEIAELLIEALSSIPGKQLWVNPDCGLKTRGYEETTASLKHMMAATKVARG"
     gene            complement(1522..2007)
                     /locus_tag="VF362_00185"
     CDS             complement(1522..2007)
                     /locus_tag="VF362_00185"
                     /inference="COORDINATES: protein motif:HMM:NF040504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family B"
                     /protein_id="HEX7589380.1"
                     /translation="MIRDAAPADAAPIAAIYNHYIEHTAITFEVDALGDDDMRARIAK
                     VQSAGYPWLVWDEAGEVRGYAYASQFRDRAAYRYSAEATVYLAPGATRRGFGSALYGE
                     LVSRLRASGTHLVVGGIALPNEASVALHESLGFTRVGTFSEIGRKFDRWIDVGFWQLR
                     L"
     gene            complement(2061..3329)
                     /locus_tag="VF362_00190"
     CDS             complement(2061..3329)
                     /locus_tag="VF362_00190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7589381.1"
                     /translation="MFAPYRSILALPGAKAFTWWGLLARAQMAMTELGILLMVQIEYG
                     SYAVAGRVVAVVAISWVFLAPIVGRLVDRFGQTATLRWGFGIAVVGRVAMVAAALTHQ
                     PEWVLMACAPLFPASGSISTYTRSRWVHLVRDDDALNTAFSLESSLDEILFIAGPAAT
                     TILATQVASWAGLGVSTAALAVGGYAFLTLRDTEPLTLRHTPPTPAERRRRLRRPRLG
                     THMLISVPAVLITTLIFAAQGALFASVDISTVAFADEFGHKSLSGPVLALFALGSLIG
                     GLVYGARVWRHTLASRLVWGVVLTGIGAATFGLAPNLLVLALLMFFTGLVVAPTMAVG
                     DGMVQALVPRNRLTEGMTWTRTGIDMGIASGSWLAGYLVQQHGASAGFVVTAVAAGAS
                     ALVALLAWRYLESTRRYEEVDEATVPAVGR"
BASE COUNT          571 a         1217 c         1208 g          477 t
ORIGIN      
        1 gggttcgctt ggcgacgcac tgcccgtcct cgtcggtacg ccggtcgagg ccatcggcat
       61 cgacttggtg cgcggcgacg tccctgtggg cgtctccctg accggtaagg tcatcgctgc
      121 gggcgtggtc gacggccaca acatctggcg cacggacctg gatgccgcgc tcgccaaagt
      181 ggccgcgtgc gaggccctcg gcggtaccgt caccgtatcc acctcgacgt ccctgctcca
      241 cgtcccgcat accaccgcag gcgaggagca tctcggcgag atcctcccgt ggctcgcgtt
      301 cgccgacgag aaggtcgccg agatcgcgac gctcgccaag gcccgcgccc agggcgagtc
      361 cgccgtggca gacgccctgg ccgcggcccg cgccgcgctg gcctcgcgcg cggcaaacgc
      421 cggcacgcac gtcgctgcgg tccgcgaggc cctcgccgcc gtgaagcccg cagactatga
      481 ccgcgcgagc gactatcgga cgcgcgacgc cgcccaggcc gagcgcttcc accttcccgc
      541 cctcgccacc acgacgatcg gctccttccc gcagaccccc gagatccgca aggtccgcgc
      601 cgccctcgtg gcgggcgaga tcacgcaggc cgtctacgag gatgcgatga agggtgagat
      661 cgcgtccgtg atcgcgcttc aggaggatct ggggctcgac gtcctcgttc acggcgaggc
      721 cgagcgtaac gatatggtcc agtacttcgc cgaactcctc gacggattcg ccgtcacgaa
      781 gaacggctgg gtccagtcct acggttcgcg ctgcacccgc ccgtcgatcc tgtggggcga
      841 cgtctcccgc ccggagccga tgacggtgaa gtgggcgacg tacgcgcagt ccctgagcga
      901 gaagcccgtc aagggcatgc ttaccggccc cgtgacgatc ctcgcgtggt ccttcgtccg
      961 cgacgaccag ccgctcggcg acaccgcgcg ccaggtcggc ctcgcgctac gccacgagat
     1021 cgcggacctc gaggccgccg ggatcggcat catccaggtg gacgagcccg cactccgcga
     1081 gctccttccc ctcaagaagc gcgatcaggc ggcgtacctc gactggtccg tcgggtcgtt
     1141 ccggctcgcc acctccggcg cggcggacgc gacccagatc cacacccacc tgtgctattc
     1201 ggagttcggc gagatcatcg atgcgatcga tggcctgggc gccgacgtca cctcgatcga
     1261 ggccgcgcgc tcccgcatgg aggtcctccc cgcgatcaag gcgcacggct tcgagcgggg
     1321 catcggcccc ggcgtctggg acatccactc gccgcgcgtg ccctcgacgc aggagatcgc
     1381 cgagttgctg atcgaggcac tctcctcaat ccccggaaag caactgtggg tcaatcccga
     1441 ctgcggactc aagacccgcg gctatgagga gacgacggca tcgctcaagc acatgatggc
     1501 cgcgaccaag gtggcgcgcg gctagagcct cagttgccag aagccgacgt cgatccagcg
     1561 atcaaacttg cgcccgatct cgctaaacgt gcccacgcgc gtgaagccga gcgactcgtg
     1621 gagagcgacg ctcgcctcgt tgggcagggc gatcccgccg acgacaaggt gggttccgct
     1681 ggcgcggagc cgggagacga gttccccgta gagcgcggag ccaaatccgc ggcgcgtcgc
     1741 gccgggcgcg aggtacaccg tggcctctgc cgagtaccgg tatgccgcac ggtccctgaa
     1801 ttggctcgcg taggcgtagc cgcgcacctc gccagcctcg tcccacacaa gccacgggta
     1861 cccagccgat tggaccttcg cgatccgcgc gcgcatgtcg tcatcgccga gcgcgtccac
     1921 ctcgaaggtg atagccgtgt gctcgatgta gtggttgtag atcgcggcga tcggcgccgc
     1981 gtccgccggg gccgcgtctc tgatcatctc cccattgtgc cggagcgttt cgcgcgcggc
     2041 cgtcacctcg cgcgcggcca tcacctcccg acggcgggga ccgtcgcctc gtcgacctcc
     2101 tcgtagcgcc gggtcgattc gagataccgc cacgcgagga gggcgacgag ggccgaggcg
     2161 ccggcggcga cggccgtgac cacgaagccg gcgcttgccc cgtgctgctg gacgaggtag
     2221 ccagcgagcc acgatccgga cgcgattccc atgtcgatgc cggttcgcgt ccacgtcatg
     2281 ccctcggtaa gccggttgcg cggcaccagg gcctggacca tgccgtcgcc cacggccatg
     2341 gttggcgcga ccacgaggcc cgtgaagaac atgagaaggg cgagcacgag cagattgggg
     2401 gcgagcccaa aggtggcggc gccgatgccc gtcaggacca cgccccagac gagtcgcgac
     2461 gccagcgtgt gacgccacac cctggcgccg tacaccaggc ccccgatgag cgagccgagc
     2521 gcgaagagcg cgagcacggg accggaaagg gacttgtgcc cgaactcgtc cgcgaatgcg
     2581 acggtggaga tgtccacgga ggcgaagagg gcgccctgtg ccgcgaagat gagggtggtg
     2641 atcaggaccg cgggtacgga gatgagcatg tgcgttccga ggcgcgggcg tcggagacga
     2701 cgcctgcgct cggcgggcgt cgggggcgtg tgcctcaacg tgagtggctc cgtgtcgcgc
     2761 agggtgagga acgcataccc acccaccgca agggcggcgg tcgagacgcc aagccccgcc
     2821 caactcgcca cctgggtcgc gaggattgtc gtggccgcgg ggccggcgat gaagaggatc
     2881 tcgtcgagcg acgactcgag ggagaacgcc gtgttgaggg cgtcgtcgtc gcggacgagg
     2941 tggacccagc gcgagcgagt gtacgtggag atgctgccgc tagcggggaa gaggggcgcg
     3001 cacgccatga ggacccactc cggctggtgg gtgagcgccg cggcgaccat ggcgacgcgg
     3061 ccgacgacgg cgatcccgaa gccccagcgc agcgtcgccg tctgtccgaa cctgtcaacg
     3121 aggcgcccga cgatcggcgc gaggaatacc cacgagatcg cgacgacggc gacgacccgg
     3181 ccagccacgg cgtaggagcc gtactcgatc tgcaccatca ggaggatgcc gagctcggtc
     3241 atcgccatct gggcgcgggc gagaaggccc caccacgtga aggccttggc acccgggagg
     3301 gcaaggatcg agcgataggg agcgaacacg ggacctcaca ctcggaggcg acgcgcgcgc
     3361 acgccgcacg tccatcacgc cacccgcgcg acgtgatcgg cgcatcgccc tcgggcgata
     3421 cgcccatggt gccatacggg gtgtcggctc gggagcgggc gttggtcctg gac
//
LOCUS       DASVKP010000009         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_249411, whole genome shotgun sequence.
ACCESSION   DASVKP010000009 DASVKP010000000
VERSION     DASVKP010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249411"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1042)
                     /locus_tag="VF362_00195"
     CDS             complement(29..1042)
                     /locus_tag="VF362_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7589382.1"
                     /translation="MTSTPHVAAESRYDHMTYRRVGRSGLLLPGVSLGLWQNFGGSAL
                     LDRQRDIILDAFDLGITHIDLANNYGPPPGAAEENFGRVLASDLRPYRDELVISTKAG
                     YLMWPGPYGEWGSRKYLLSSLDASLKRMRLEYVDIFYHHRPDPETPIEESMMALDRAV
                     RSGKALYAGVSNYGPEGTAEAARILRELGTPMLIHQPRYSMFDRRPEEGLLDVLGDEG
                     VGCIVFSPLAQGLLTDRYLGGIPEGSRATHSPFLSQKQISKTYLERASALNDIAKGRG
                     QTLAQMAIQWVLRDDRVTSALVGASSVEQLRANVAALNSPPLTAAEIEAIEPHAVHGT
                     EHL"
     gene            1304..2287
                     /locus_tag="VF362_00200"
     CDS             1304..2287
                     /locus_tag="VF362_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589383.1"
                     /translation="MVAGPFPDIALRQGVANDDEVVVNYMRESDDAVAVYREGAWATW
                     GVVPAAAPGLDNGGVVQAIALSDVGPVFAVTVEADALDYSLYFWRDGDAAPSLVQHLV
                     SNFFWPEYAVMGSDLYLTAERGDLSCIDRLDLTSASPLATAKQMSCGVAGSLLFSPSL
                     DEGTLVFGERPASDECTTELRRVDLASGAETVVDSGPCAFMGLGSAAFSAWTQVPPDR
                     SAPNAWNAAPLWADVGGDVTQVGETVANSPIACGGALYFVGGGSAESRLKRWEPGGEA
                     VTVYRAPPGASVLDLSCAAGRLSAFIGDADGTSLLTTPPPDWSAGVPSDAS"
     gene            complement(2284..>2800)
                     /locus_tag="VF362_00205"
     CDS             complement(2284..>2800)
                     /locus_tag="VF362_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NDP-hexose 4-ketoreductase"
                     /protein_id="HEX7589384.1"
                     /translation="SVAHEGGHTYDAMVKLVNSKLKDHFKPEFLNRVDNVVVFPQLTQ
                     DEIIQIVDLMIARLDERMRDKDMSIVLTDAAKRLLAEKGYDPVLGARPLRRAIQRDIE
                     DHLSEKILFGDLVAGQIVKVDAIGSGDDGEFTFEGVSRDELDEPRGSKDKAKVKAAAT
                     AGTDDETASSS"
BASE COUNT          412 a          889 c         1017 g          482 t
ORIGIN      
        1 ggcgttccgg atcagggagt cctggtggct agaggtgttc ggtgccgtga acggcgtggg
       61 gctcgatggc ctcgatctcc gcggcggtga gcggcgggga gttgagagcc gcgacgtttg
      121 cgcgcagttg ctccaccgac gacgcgccga cgagcgcgga ggtgacccgg tcgtcgcgaa
      181 ggacccactg aatcgccatc tgcgcgagcg tctggccccg ccccttggcg atgtcgttga
      241 gggcgctcgc gcgctcgaga taggtcttcg agatctgctt ctgggacagg aacgggctgt
      301 gggtggcgcg cgagccctcg ggaatcccgc cgaggtagcg gtccgtgagc aatccctggg
      361 caagcggcga gaacacgatg catccgactc cctcgtcgcc gaggacgtcg agaagaccct
      421 cctcggggcg gcggtcgaac atcgagtacc ggggctggtg gatgagcatg ggcgtgccga
      481 gctcacggag gatccgtgcc gcctccgcgg tgccctccgg cccgtagttc gaaaccccag
      541 cgtagagggc cttgcccgag cggacggccc ggtccagcgc catcatcgac tcctcgatgg
      601 gagtctccgg gtccgggcgg tggtggtaga agatgtcgac gtattcgagc ctcatgcgct
      661 tcagcgacgc gtcgagcgaa ctgagcaggt acttgcggga gccccattcg ccgtaggggc
      721 cgggccacat gaggtacccc gccttggtgg agatgacgag ttcgtcgcgg tacggacgca
      781 ggtcgctcgc gagaacgcgg ccgaagttct cctcggcggc cccaggcggc ggaccgtagt
      841 tgttcgcgag gtcgatgtgg gtgatcccga ggtcgaaggc gtcgaggatg atgtcgcgtt
      901 gccggtcgag gagggcggat ccgccgaagt tctgccacag gcccagcgac acgcccggga
      961 gcaggaggcc cgaacgtccg acgcggcggt aggtcatgtg gtcgtagcgg gattctgcgg
     1021 cgacgtgggg ggtggaggtc atggactcag actactgacg ccattcagcg agcgggaatc
     1081 gagccgcggc cccataattg attcctgagg ggaccaaacg acgggggcaa tcacgggatt
     1141 cgtgacgcgg ggacgacgcg ccgtcttggt ggccgctacg gcgctggcac tgatcgcggg
     1201 cggctgttcg gcccctgccc cgacgcggtc gccgtcgcca gtgtctccga cgccgaccga
     1261 gaccccttcg ccaacggtgt cgtcggcgct ggagccattt gagatggtcg cggggccctt
     1321 ccccgacatc gcgctccggc aaggggtggc gaacgacgac gaggtcgtcg tgaactacat
     1381 gcgcgagtcc gatgatgccg tcgccgtgta ccgagaaggc gcgtgggcga cctggggggt
     1441 ggtgcccgcc gcggccccgg gcttagacaa cgggggcgtc gtccaggcga tcgcgctctc
     1501 ggacgtcggc ccggtcttcg ccgtcacggt cgaggcggac gcgcttgact actcgctcta
     1561 cttctggcgg gacggggacg ccgcgccgag cctcgtccag cacctggtct ccaacttctt
     1621 ctggccggag tacgccgtga tgggaagcga cctgtacctg acggccgagc gcggggactt
     1681 gtcctgcatc gaccgcctcg acctcacgtc ggcgagtccg ctcgcgacgg ccaagcagat
     1741 gtcgtgcggg gtcgcaggct ctttgctgtt ctcgccgtcg ctcgacgagg gaaccctcgt
     1801 gttcggtgag cgccccgcga gtgatgagtg cacgacggag ctccgcagag tggacctcgc
     1861 ctcgggcgcc gaaacggtcg tcgattcggg accgtgcgcg ttcatgggac tgggcagcgc
     1921 cgcgttctcc gcgtggacgc aggtgccgcc cgaccggtcc gcaccgaatg cgtggaatgc
     1981 ggcgccgttg tgggcggatg tcggcggcga cgtcactcag gtgggcgaga ccgtcgcgaa
     2041 ctcccccatc gcgtgcggcg gggccctcta cttcgtgggt gggggttctg ccgagagtcg
     2101 cctcaagcgc tgggagcccg gaggagaggc cgtcaccgtc tatcgagcgc cgcccggggc
     2161 gtccgtcctc gacctttcgt gcgcggctgg acgcctctcg gccttcattg gcgacgccga
     2221 cggaacctcg ttgctcacca cgccgccgcc ggactggtcg gcgggcgttc cgagcgacgc
     2281 gagctaggag gaacttgccg tctcgtcgtc ggtgcccgcg gtcgccgcgg ccttgacctt
     2341 cgccttgtcc ttggaaccgc gcggctcgtc gagttcgtcg cgggagacgc cctcgaacgt
     2401 gaactcgcca tcgtcacccg acccgattgc gtcgaccttc actatctggc ccgcgacgag
     2461 gtcgccgaag aggatcttct cggacaggtg atcctcgatg tcgcgctgga tggcgcggcg
     2521 aagcggccgc gcgcccagga cggggtcgta gcccttctcc gcgagcaacc tcttggccgc
     2581 gtcggtgagc acgatcgaca tgtccttgtc acgcatgcgc tcgtcgaggc gagcgatcat
     2641 gaggtcaacg atctggatga tctcgtcctg cgtgagctgc gggaacacca cgacgttgtc
     2701 gacgcgattg aggaactcgg gcttgaagtg atccttgagc ttggagttca ccaacttgac
     2761 catcgcgtcg taggtgtggc caccctcgtg ggcgacggag
//
LOCUS       DASVKP010000010         2894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_578719, whole genome shotgun sequence.
ACCESSION   DASVKP010000010 DASVKP010000000
VERSION     DASVKP010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578719"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VF362_00210"
     CDS             complement(<1..922)
                     /locus_tag="VF362_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEX7589385.1"
                     /translation="MSPQTNSTAPAFPGGTILGYPRIGRNRELKKALESFWKGATTAV
                     DLESAARDIRLANLERMAELGLGRGDSSIPSNFSFYDHMLDASVAFGCVPSRFKEFES
                     EHGGYDLAGYSTIARGQGDKAPLEMTKWFDSNYHFLVPEIGPETTFELGSIRWIDEFV
                     EARNAGFLTRPVVTGPLTFLYLAKPSDEAPRGFRPIDRLADLLPVYEKLLGEFKIAGA
                     QWVQIDEPILVADFDAPRADVLAAAKAAYAAVAGVTERPSVFVAAPYASLDEALPVLA
                     ATGVEAISLDLVRGTVPRGVNLAGKTVVAGV"
     gene            1109..1531
                     /locus_tag="VF362_00215"
     CDS             1109..1531
                     /locus_tag="VF362_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7589386.1"
                     /translation="MTETSKRQVTLTRESKGVYIATNARGGTLRFGPEARDGFSPVEL
                     LLAAVAGCSGIDVDYVTSRRAEPLRFDVVSEATQENGDDGNILRDVTVTFHLEFPPGE
                     DGDKARDRIDAALKASHDTTCTVSRTLEAATPVVLRKA"
     gene            complement(1555..2445)
                     /locus_tag="VF362_00220"
     CDS             complement(1555..2445)
                     /locus_tag="VF362_00220"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX7589387.1"
                     /translation="MTIRDLLRAPAPTLSFELFPPRDSDAQEALWETIARLAEARPDF
                     ASITYGASGTTRRSSREVVRRLADAHTIRPLAHLTCVDQSRAEVTAVIEEFLAEGVRD
                     FLALRGDPPAGQPDWRPHPDGLLYAYQLVAHIREVVAARGIAEGEFSVGVAAFPAAHA
                     EERWRQQGLDVLRAKQDAGADFAVTQVFYEPAHWEALAADALAAGITLPILPGIIPLL
                     STGRAARLEQLTGVSVPESVLRALAAEADADAARIAGIAHAARLARDLVSAGAPGIHV
                     YTFNRHEAALELVGAAGLRR"
     gene            2577..>2894
                     /locus_tag="VF362_00225"
     CDS             2577..>2894
                     /locus_tag="VF362_00225"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX7589388.1"
                     /translation="MSEVRTAGERCFGTGDAAMERYHDEEWGRAVRDDAQLLERIALE
                     GFQSGLSWATVLHKRENLRAAFAGWDARGIAAFGDDDVARLMDDGGIIRNRAKIASTI
                     TNAR"
BASE COUNT          465 a          979 c         1039 g          411 t
ORIGIN      
        1 ccacaccggc gacgacggtc ttgcccgcga ggttcacgcc cctgggcacg gtgccgcgga
       61 cgaggtcgag cgagatcgcc tcaacgcccg tcgcggcgag gacggggagg gcctcgtcga
      121 gcgaggcata cggcgccgcg acgaacaccg aggggcgttc cgtcaccccg gcgaccgcgg
      181 cgtacgccgc cttggcggcc gcaagcacgt ccgcgcgagg ggcgtcgaag tcggcgacga
      241 ggatcggctc atcgatctgc acccattgcg cacccgcaat cttgaactcg cccaggagct
      301 tctcgtagac cggaagaagg tccgcgaggc ggtcgatggg gcggaatccg cggggcgcct
      361 cgtcggaggg cttggccagg tagaggaacg tgagcggacc ggtgacgacc gggcgggtga
      421 ggaagccagc gttgcgcgcc tcgacaaact cgtcgatcca ccggatcgag ccgagttcga
      481 aggtcgtctc ggggccgatc tctggaacga ggaagtggta gttggaatcg aaccacttcg
      541 tcatctcgag cggcgccttg tcaccctggc cgcgggcgat ggtcgagtac ccggcgaggt
      601 cgtagccgcc gtgctcgctc tcgaactcct tgaagcgcga aggaacgcaa ccgaacgcga
      661 cggatgcgtc gagcatgtgg tcgtagaacg agaagttcga cgggatggac gagtcgccgc
      721 gacccaggcc gagctccgcc atgcgctcca ggttcgcgag gcgaatgtcg cgcgcggcgg
      781 actcgaggtc caccgcagtg gtcgcgccct tccagaagga ctcgagcgcc ttcttgagct
      841 cgcggttcct cccgatacgc ggatagccga ggatggtgcc gccgggaaag gcgggcgcgg
      901 tgctgttcgt ctgagggctc atgtgtcttc caacgatcgt gggtgcgcgg cgggctcgcc
      961 taagcgcata tggcgatggc tcgccaatga ggtgcctagg agtctaccgt gggcgctttc
     1021 gatcgacgaa cctgagccgc tcagcgctcc gcgcgcgcta ttcgcttgag gctatggcga
     1081 tccgagcccc ccgtgaaagg atcgccccat gaccgaaacc tcgaagcgcc aggtgaccct
     1141 cacccgcgag tccaagggcg tatacatcgc gaccaacgcc aggggcggga ccctccggtt
     1201 cgggccggag gcgcgggacg ggttctcccc cgtcgagctc cttctcgccg ccgtcgcggg
     1261 ttgctcgggg atcgacgtgg actacgtgac ctcgcggcgc gccgagccgc tccgcttcga
     1321 cgtcgtctcc gaggcgaccc aggagaacgg ggacgacggc aacatccttc gcgacgtgac
     1381 cgtcaccttc cacctggagt tccccccggg cgaggacggg gacaaggcgc gcgaccgcat
     1441 cgacgccgcc ctcaaggcct cccacgacac gacctgcacc gtgagccgga ccctcgaggc
     1501 cgcgacgccc gtggtcctca ggaaggccta ggctccacca gggcgccagc cttgctaacg
     1561 cctcagcccc gccgccccga cgagttcgag cgcggcctcg tggcggttga acgtgtagac
     1621 gtgtatgccc ggagcgccgg cggacaccag atcccgcgcg agccgggccg cgtgcgcgat
     1681 cccggcgatt cgcgccgcgt cggcgtcagc ctcggccgcg agcgcacgga gaaccgactc
     1741 cggcacgctc accccggtca gttgctccag gcgagcggcg cgcccggtgc tgagcagcgg
     1801 gatgattccg ggaaggatcg ggagcgtgat cccggcggcg agcgcgtcgg cggcgagcgc
     1861 ctcccagtgc gcgggctcgt agaagacctg agtgacggcg aagtcggcgc ccgcgtcctg
     1921 cttggcgcgt agcacgtcga ggccctgctg gcgccaccgc tcctcggcgt gggcggcggg
     1981 gaacgccgcg accccgacgg agaactcgcc ctcggctatc cctcgtgccg cgaccacctc
     2041 gcgaatgtgg gcgacgagct ggtacgcgta caggaggcca tcgggatggg ggcgccaatc
     2101 cggttgcccc gcgggagggt cgccgcgcag cgcaaggaag tcgcgcacgc cctcggcgag
     2161 gaactcctcg atgacggcgg tgacctcggc gcgcgactgg tcgacacatg tgaggtgcgc
     2221 gagcggccga atcgtgtgcg cgtcggcgag gcgcctcacc acctccctgc tcgatcgtcg
     2281 ggtcgtcccg ctcgccccgt aggtgatcga ggcgaagtcc ggccgggcct cggcaaggcg
     2341 cgcgatcgtc tcccagagcg cttcctgcgc gtcggagtcg cgcggaggga agagttcaaa
     2401 ggacaaggtc ggcgctggcg cccggagcag atcgcggatg gtcatctcgg ttgccaaggc
     2461 tagcctcccg gggccaggga gccaacctgt gccccaaggt cgtcctccgt ccccttcctt
     2521 cccgggaccc cggcaactgg ctcgacaccg tcggccgcac ggcccagact gtccccatga
     2581 gcgaggtgcg gacggcgggc gaacggtgct tcggcaccgg ggacgccgca atggagcggt
     2641 atcacgacga ggaatgggga cgcgcggtca gggacgacgc gcaactgttg gagcgcatcg
     2701 cgctcgaggg atttcagtcc gggctgagtt gggcgacggt gcttcacaag cgtgagaacc
     2761 tccgcgcggc gttcgccggc tgggatgctc ggggcatcgc ggcctttggt gacgacgacg
     2821 tggcgaggct catggacgac ggcggaatca tccgcaaccg cgcgaagatc gcctcgacca
     2881 tcaccaacgc gcga
//
LOCUS       DASVKP010000011         4709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_332175, whole genome shotgun sequence.
ACCESSION   DASVKP010000011 DASVKP010000000
VERSION     DASVKP010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332175"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VF362_00230"
     CDS             complement(<1..1031)
                     /locus_tag="VF362_00230"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX7589389.1"
                     /translation="MTLPKPRAAIAALPAYVPGARPSGAGAPPIKLSANEVAFAPLPS
                     VREAIAGAGEFTNRYPDMVASEVAEGIAARWSVGAESVVVAGGSVAVLGHALHAYCDV
                     GDEVVYAWRSFEAYPILAQIAGAASVRVALDPDARHDLAAMASALSPRTKVVVLCTPN
                     NPTGPAIRRDEFESFMAVVPRGVVVVLDEAYAEFVRDPAAVDGRAALAAHPNLLVMRT
                     FSKAYGLAGLRVGYAIGTPDLVAPIRACVTPFSVSGVAQAAALASLVAEDELLARVDA
                     VVAERERVLARLAEDGWRVPDAQGNFVWLAAGAETGALAAYLEGRAPSILVRPFSGEG
                     VRITVGSPQ"
     gene            1069..1452
                     /locus_tag="VF362_00235"
     CDS             1069..1452
                     /locus_tag="VF362_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX7589390.1"
                     /translation="MVFLFRSVITAVAVWIVTLLNLDVVVTGGNDTWWSRALVYLGVG
                     AVLVAVNMIVKPIVNLLTLPLRILTLGLFALVINWAMLELAAWLTSHLPIGTLELGGF
                     WKTLLAAFLIAIISVILGVRKKKRD"
     gene            complement(1473..2597)
                     /locus_tag="VF362_00240"
     CDS             complement(1473..2597)
                     /locus_tag="VF362_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589391.1"
                     /translation="MWDGLWAAARRAIRGEIAVGETVALVGTAVAFPASLLVPGGPPA
                     LTGIPAPEPTSLLDASGLEAALAAGHDLPLVPALLGWSVVDLLTLCLMLSAMGWSELL
                     GNPHGLAVEESERRITSAPAGAADLIERVGGLYPTGGADRGTAHVAIECIEHPDGTRA
                     WIVEIPGTQSFLPNGGSNPFDFTADLQLMSGQTSDVMIGVAAAMRDAGIPAGDPVMLV
                     GHSLGGIGAMALASNGSFASRYNVQAVVTAGSPVARFEPVGGVSVLSLENSTDIVWAL
                     DGAPNPDRAEWITVTHDLRASEDPFDRAAAASIVGSHEPETYVRTAGAFDSASGSSAA
                     AWREQNAAFLADGTAVSIRTTYDITRGVEGATIPSVADGR"
     gene            complement(2769..2930)
                     /locus_tag="VF362_00245"
     CDS             complement(2769..2930)
                     /locus_tag="VF362_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589392.1"
                     /translation="MSDIAIVGGAGGTHARTEDLARVSAVLDGARDRMEGVAAWARAA
                     RSDLRSAQW"
     gene            complement(2927..3130)
                     /locus_tag="VF362_00250"
     CDS             complement(2927..3130)
                     /locus_tag="VF362_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589393.1"
                     /translation="MTHPAADPSLAVSVALSALRQADAIPWTSDLAATFRLRVDAVER
                     LVVGVSYRVDCAGAALARLAVAA"
     gene            3263..>4709
                     /gene="purB"
                     /locus_tag="VF362_00255"
     CDS             3263..>4709
                     /gene="purB"
                     /locus_tag="VF362_00255"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX7589394.1"
                     /translation="MTTPWRTLADLSPALALGPLDGRYRPVVAPLVDHLSEAALNRAR
                     LHVEVEWLIHLSDTGAVPGVAPLTDADRAYLRAIVEAFGADEIAELAEAERVTAHDVK
                     AIEYFLKKRLADAPKALGAAALGTTALGTTALGTTALPALAELVHFGCTSEDINNLAY
                     ALTIRSAIAQVWLPAATALTDDVATLAREHAAQPMLSRTHGQPATPTTLGKELAVLAR
                     RLRRQLARIGRQEYLGKINGATGTYGAHLAAVPGTDWIATSRSFVEGLGLAWNPLTTQ
                     IESHDWQAELYSDVARFNRILHNLATDVWTYVSLGYFAQVRGQGTVGSSTMPHKVNPI
                     RFENAEANLEISCALLDSLGSTLVTSRLQRDLTDSTTQRNIGVALGHSLLAIDNVKRG
                     LAGLDANPAAMAADLDANWEVLGEAIQSAMRAAGLAGAPGMEEPYERLKELTRGRRVG
                     GDDIRAFVQGLGLPADVEARLLELTPATYVGA"
BASE COUNT          738 a         1749 c         1585 g          637 t
ORIGIN      
        1 tcttgaggcg agcctacggt gatgcgtacc ccttcgccag agaacggccg gacgaggatc
       61 gacggcgccc tcccctcgag gtacgccgcg agcgcgccgg tctcggcgcc cgcggccagc
      121 cacacgaagt tcccctgggc gtccggcacg cgccacccgt cttcggccag ccgcgcgaga
      181 accctctcgc gttctgcgac gacggcatcg accctcgcga gaagctcatc ctccgcgacg
      241 agggatgcga gcgccgcggc ctgcgccacc cccgagacgg agaagggcgt cacgcacgcg
      301 cggatcggcg cgacgagatc cggcgtgcca atcgcgtagc cgactctcag ccccgccagc
      361 ccatacgcct tggagaaggt tcgcatgacg aggaggttgg ggtgggcggc gagcgccgcg
      421 cgcccgtcga ccgccgcggg gtcgcgcacg aactccgcgt acgcctcgtc gaggaccacc
      481 acgacgcctc gcggcaccac cgccatgaag gactcgaact cgtcgcgccg aatcgccggg
      541 ccggtcgggt tgttcggcgt gcataggacg acgaccttgg tgcgaggtga gagggcgctg
      601 gccatggccg cgaggtcgtg ccgcgcgtcc ggatcgagcg cgacccgcac cgacgccgca
      661 ccggcgatct gagcgaggat cggatacgcc tcgaacgacc tccacgcgta gacgacctcg
      721 tcgcccacgt cgcagtacgc gtggagggcg tggccaagga cggcgacgga gccgccggcg
      781 acgacgaccg actccgcacc caccgaccag cgggcggcga tgccctctgc cacttcggag
      841 gccaccatgt cggggtatcg gttggtgaac tcgcccgccc ccgcgatcgc ctcgcggacg
      901 gagggaaggg gggcgaacgc cacctcgttc gcggagagtt tgatcggcgg cgcccccgcc
      961 ccggacggac gggcccccgg aacgtaggcc ggaagtgccg caatcgcggc gcgcggcttg
     1021 gggagcgtca taccgaaagc atgccaccgc cgtggaacaa tcgtgggcat ggtcttcctc
     1081 ttcaggtctg tcatcaccgc ggtcgccgta tggatcgtta cccttctgaa cctcgacgtc
     1141 gttgtcaccg gcggaaacga cacgtggtgg agccgagcgc tcgtctacct cggggtcggg
     1201 gcggtcctcg tggccgtcaa catgatcgtg aagcccatcg tcaacctcct caccctcccg
     1261 ctacggatcc tgacgctcgg cctcttcgcg ctcgtcatca actgggcgat gctggaactc
     1321 gcggcatggc tcacgagcca ccttccgatc ggaaccctcg aacttggggg attctggaag
     1381 acccttctgg cggcgttcct gatcgcgatc atcagcgtga tcctgggcgt caggaagaag
     1441 aagcgcgact agttccgctg gggctacggc gactatcggc catcggcgac cgacggaatg
     1501 gtcgcgccct cgacgccccg ggtgatgtcg taggtcgtcc ggatcgacac cgccgtgccg
     1561 tccgcgagga acgccgcgtt ctgctccctc catgccgcgg cggacgaccc gctcgcggag
     1621 tcgaacgccc ccgccgtgcg cacgtaggtt tcgggctcgt ggctacccac gatgctcgcg
     1681 gccgccgcgc gatcgaacgg atcctccgag gcccgaagat cgtgggtgac cgtgatccac
     1741 tccgcgcgat ccgggttggg tgcgccgtcg agggcccaca cgatgtcggt ggagttctcc
     1801 aggctcagaa ccgagacgcc gccgacgggc tcgaagcgcg cgacggggga cccggcggtg
     1861 acgaccgcct ggacgttgta tcgcgaggcg aaggagccgt tgctcgcgag cgccatggcc
     1921 ccgattccgc ccaggctgtg cccgacgagc atcaccgggt cgcccgcggg aattcccgcg
     1981 tcgcgcatgg cggcggcgac cccgatcatg acatcggacg tctggcccga catcaactga
     2041 aggtccgcgg tgaagtcgaa cgggttggat cccccgttcg ggaggaagct ctgggttccg
     2101 gggatctcga cgatccacgc gcgcgttccg tccggatgct cgatgcattc gatcgcgacg
     2161 tgggccgttc ctcggtccgc gccacccgtc gggtagagcc ctccgactcg ctcgatgagg
     2221 tccgccgctc ccgccggagc gctcgtgatg cgccgttccg actcctccac ggcaagcccg
     2281 tggggattgc cgaggagttc cgaccagccc atcgccgaga gcatgaggca gagcgtcagg
     2341 agatcaacga ccgaccaacc gagcagggcg gggacgagcg gcaggtcgtg ccccgccgcg
     2401 agtgccgcct cgagtccgct ggcgtcgagt agcgacgtcg gttcgggcgc ggggatcccg
     2461 gtgagcgccg ggggacctcc ggggacgagg agcgacgcgg gaaaggccac ggcggtaccg
     2521 acaagcgcca ccgtttcgcc gacggcaatc tcgcccctga tcgcccgacg cgccgcagcc
     2581 cacagtccgt cccacaaccc cccgtcccac ggccgtccgg cgccccgctc ggcgccctcc
     2641 agcaggcgca ccgtttccgc gagcccccgc gctatgtcct caatttcgcg cgcgaccgcg
     2701 tcggcgccac cggctcccag attgacccaa tcgagcgcgc cctcgacatg gtccgcaacg
     2761 atcgcgtctc accactgcgc cgagcgcagg tcggacctcg cggcccgagc ccaggccgcg
     2821 acgccctcca tgcggtcgcg cgcgccgtcc aggaccgcgc tcacgcgggc gagatcctct
     2881 gtccgggcgt gcgtgccacc ggcgccgccg acgatggcga tgtcgctcat gcggcgacgg
     2941 cgaggcgcgc cagcgccgcc ccggcacagt caacgcgata gctcaccccc acgaccaggc
     3001 gctcgaccgc gtccacgcgc aaccggaacg tcgcggccag gtcggaggtc cacgggatcg
     3061 cgtccgcctg gcgaagagcg gacagggcga ccgagaccgc gagggaggga tctgcagcgg
     3121 gatgagtcac ggcgccaggc taggaacaac cccggcggcg cggccgggcc gccggccacc
     3181 tgtgcaagag ccgcgctcgc cggacgcctc ggaacatcac ccggttgggc ggcgaaaggg
     3241 cgagccgtgg aacaatggct ccgtgaccac gccatggcgg acgctcgctg acctcagtcc
     3301 cgccctagcc ctgggacccc tcgacggccg ctaccgaccc gtcgtcgcgc cgctcgtcga
     3361 tcacctgagc gaggccgccc tcaaccgcgc ccggctccac gtagaggtgg agtggctcat
     3421 ccacctgagc gacacgggcg ccgttcccgg ggtcgcaccg ctcaccgatg ccgatcgcgc
     3481 ctatctgcgc gccatcgtcg aggcgtttgg cgcggacgag attgccgaac tcgccgaggc
     3541 ggagcgggtc acggcgcacg acgtcaaggc gatcgagtac ttcctcaaga agcgcctcgc
     3601 ggatgcgccc aaggcgctcg gggccgctgc actcgggacc acggcgctcg gcacgaccgc
     3661 acttgggacg accgcgctcc ccgccctcgc cgagctcgtc cacttcgggt gcacctccga
     3721 ggacatcaac aacctggcgt acgcgctcac cattcgcagc gcgattgccc aggtgtggct
     3781 ccccgcggcg accgcgctca cggacgacgt cgcgacgctg gcgcgggaac acgcggccca
     3841 gccgatgctc tcccgcaccc acggccagcc tgccaccccg acgacgctcg gcaaggaact
     3901 cgcggtcctc gcccgtcggc tccggcgtca actcgcgcgc atcgggcgcc aggagtacct
     3961 gggcaagatc aacggcgcca cgggcaccta tggcgcgcac ctcgccgcgg ttccgggcac
     4021 ggactggatc gccacgagcc ggtccttcgt cgaggggctc ggcctcgcgt ggaacccact
     4081 caccacccag attgagagcc acgactggca ggctgagctc tactcggacg tcgcgcgctt
     4141 caaccgaatc ctccacaacc tcgcgaccga cgtctggacg tacgtctcgc tcgggtactt
     4201 cgcgcaggtg cgcggccagg gcacggtcgg ctcgtccacg atgcctcaca aggtcaaccc
     4261 gatccgcttc gagaacgccg aggcgaacct cgagatttcg tgcgccctgc tcgactcgct
     4321 cggctcgacc ctggtgacga gccgcctcca gcgggacctc accgactcca cgacgcagcg
     4381 caacatcggc gtcgcgctcg gccactcgct cctcgcgatc gacaacgtca agcgcggcct
     4441 cgcgggcctc gacgccaacc ccgccgccat ggccgcggac ctcgacgcga actgggaggt
     4501 cctgggcgag gcgatccaat ccgcgatgcg cgccgcgggg ctcgcgggcg cgccagggat
     4561 ggaggagccc tacgagcggc tcaaggaact cacgcgcggc cgcagggtcg ggggagacga
     4621 catccgggcc ttcgtccagg ggctcgggct tccggccgac gtcgaggccc ggctcctcga
     4681 gctcactccc gcgacgtacg tcggggcag
//
LOCUS       DASVKP010000012         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1400955, whole genome shotgun sequence.
ACCESSION   DASVKP010000012 DASVKP010000000
VERSION     DASVKP010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400955"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VF362_00260"
     CDS             <1..767
                     /locus_tag="VF362_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEX7589395.1"
                     /translation="KEIDMDDASRETRISAAFVAVADTLTTDFDVVDLLHTLVQECVE
                     ILGMKAGGLMLADGAGELHLMTSTSEAAAFVEVMQLAAASGPCIDCFTGGVAVSVSDI
                     EDPDSHWPEFASASLAQGFHSALATPMKLRGRIIGTMNLFGATTGKVSARNAAVAQAL
                     ADVATIGILQERLIHEGHLVEEQLHNALDSRIMIEQAKGVLAHSLSLSMDDAFGLLRK
                     YARDGNLTIRSVAQRVSDRELAVQEIAKASGHATSL"
     gene            complement(994..1815)
                     /locus_tag="VF362_00265"
     CDS             complement(994..1815)
                     /locus_tag="VF362_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX7589396.1"
                     /translation="MISAIAFDLDDTLAPSKSKVPDELGHLLMEIARHMPVCVISGGR
                     FEQFDTQLLGSLSGTPEEFANLHLMPTCGTKYFRYESGHWEMVYTEDLTPDEVRRAKA
                     SLERGAKSLGLWEPDSAVFGARIEDRGSQVTFSALGQEAPVALKHAWDASGQKREVLR
                     ECVQHDLPDLEVRAGGSTSIDVTRRGIDKAYGIREFARIIGVDFPDILFVGDRLEPGG
                     NDFPVIALGVATRAVTGPDDTTAFARGFVVQLEEFVVQRDGRAELRRGRDRPGGG"
     gene            2025..2186
                     /locus_tag="VF362_00270"
     CDS             2025..2186
                     /locus_tag="VF362_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589397.1"
                     /translation="MTVRRQTVSQEAIRRSVARSTKASARLESRVVPEGFVRSATASA
                     YIAARKPRG"
     gene            2282..>2599
                     /locus_tag="VF362_00275"
     CDS             2282..>2599
                     /locus_tag="VF362_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589398.1"
                     /translation="MKAALYDLEQAVEDEVISGLLTQVWDGSLGLDDVLSDGFLRELH
                     GQMYGDIWTWAGRFRTKDTNVGVPSETIAMELRTAIETVRYRWEHTDDWGARELGMAV
                     HAET"
BASE COUNT          456 a          882 c          843 g          418 t
ORIGIN      
        1 agaaggagat tgacatggac gatgcctcac gtgaaacgcg cattagcgcc gcattcgttg
       61 ccgtggccga taccctgacc accgacttcg acgtggtgga ccttcttcac accctcgtcc
      121 aggagtgcgt ggagatcctc ggcatgaagg ccgggggact gatgttggcc gacggcgcag
      181 gtgaactcca tctgatgacc tccacgagcg aggctgcggc cttcgtcgag gtcatgcaac
      241 tcgccgccgc ctcgggtccg tgcatcgatt gcttcaccgg aggtgtcgcc gtgtccgtca
      301 gcgacatcga ggacccggac tcccactggc ccgaattcgc tagcgcctcg ctggcgcaag
      361 gcttccactc ggccctggcg accccgatga aactgcgcgg aaggatcatc gggaccatga
      421 acctctttgg cgccactacg gggaaggtca gcgccaggaa tgccgcggtc gcgcaggccc
      481 ttgccgacgt cgcgacgatc ggcatccttc aggaacgcct catacacgag ggccatctcg
      541 ttgaggagca actccataac gcgctggaca gccgcatcat gattgagcag gcgaagggag
      601 tccttgccca ctctctgtca ttgagcatgg atgatgcctt cgggctgctg cggaagtatg
      661 cccgcgacgg gaacctcacc attcgttccg tcgcgcaacg cgtgagcgac cgagaactcg
      721 ccgtgcagga gattgccaag gcctccggac acgcaacctc cctgtagcaa atcccgaacg
      781 atgctaccgt ctatgcagtt gctctagtcc tcggcagcgg gtcacttccg cgcgtagcta
      841 aggaacatca tgatgagcga ttccattgcg ggagccttgc cggttgatgt cgtatgggat
      901 ctagacactg ccccgatcgg agcacccgtc ccgcgccgca tcatgagggc gcgccgcgat
      961 gcggcagagg ctctcgatct tgcctccgcg agttcagccc ccacctggtc ggtcgcgccc
     1021 gcgccggagt tcggctcgac cgtcccgctg gacgacgaac tcttctaact gaacgacgaa
     1081 gccccgcgcg aacgccgtgg tgtcgtcggg accggtgacc gcgcgcgtcg cgacgccgag
     1141 ggcgatgaca gggaagtcgt tgcccccggg ctcgaggcgg tccccgacga agaggatgtc
     1201 cgggaagtcc accccgatga tgcgcgcgaa ctcgcggatg ccgtaggcct tgtcgatccc
     1261 gcgccgcgtc acgtcgatcg aggtcgagcc gcccgcgcgc acctcgagat cgggtaggtc
     1321 gtgctggacg cactccctca agacctcgcg cttctggccg gacgcgtccc aggcgtgctt
     1381 gagcgccacg ggcgcctcct ggccgagcgc ggagaacgtc acctgcgagc ctcggtcctc
     1441 gatgcgcgcg ccgaacaccg cagagtccgg ctcccacagc ccgagggact tcgcgccgcg
     1501 ctcgagcgac gccttggccc tgcgcacctc gtcgggcgtc aggtcctcgg tgtagaccat
     1561 ctcccagtgc cccgactcgt acctgaagta cttcgtcccg caggtgggca tcaggtggag
     1621 gttcgcgaac tcctccggag tgccggacag cgagccgagc agctgcgtgt cgaactgctc
     1681 gaagcgcccg ccggagatca cgcacactgg catgtgccgg gcgatctcca tgaggaggtg
     1741 cccgagttcg tcggggacct tggacttgga cggggcgagg gtgtcatcga ggtcaaacgc
     1801 gatcgcggag atcatcggac tcccgttccg gacacaaacg ccgttgtctg cacgcgaccc
     1861 atagtagggc acccgacgcg caggctcgcc cgcggcgtcg tgcctgcgcc tccccgagga
     1921 catcgccccc gggagggggc tccgtctatc gggggagaat ccccagcgct agcatggaac
     1981 ttcgagcatt cgagcacttg cgcacccaaa cgtaggagga ggtgatgacc gtgagacgcc
     2041 agaccgtgag ccaggaagcg atcaggcggt ccgtcgcccg ctccaccaag gcgtcggcgc
     2101 gcctggaatc gcgcgtggtg ccggagggct tcgtccgctc cgccacggcc agcgcgtaca
     2161 tcgccgccag aaagccccgg ggctgaaccc cctcgacccc aactacggcg ccacgcccgt
     2221 cgacgacgag gacgcgcggg cgcttgttcc tgccgcgcgc gaggccctcg gcgagcccct
     2281 gttgaaggcc gccctctacg acctggaaca ggccgtcgag gatgaggtca tctcggggct
     2341 actcacccag gtatgggacg ggtcgctggg cctcgacgac gtcctcagcg acggcttcct
     2401 ccgcgaactc cacggccaga tgtacggaga catctggacc tgggcggggc ggtttcgaac
     2461 caaggacacg aacgtcggcg tgccatcgga gaccattgcg atggagttgc gcacggccat
     2521 cgagaccgtc cgataccgct gggaacacac cgacgactgg ggggcgcgcg aactcggaat
     2581 ggctgtccac gccgagacc
//
LOCUS       DASVKP010000013         2598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_168055, whole genome shotgun sequence.
ACCESSION   DASVKP010000013 DASVKP010000000
VERSION     DASVKP010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168055"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VF362_00280"
     CDS             complement(<1..378)
                     /locus_tag="VF362_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEX7589399.1"
                     /translation="MSGDRGASDLVIVALGGGHGLYASLSALRQLSENLTAIVTVADD
                     GGSSGRLRDELRVPPPGDLRMALSALCEDTEWGHMWRDVLQHRFVTDGPLDGHSLGNL
                     LIAGLWNRTGDVVEGLDWVARLLR"
     gene            complement(375..1298)
                     /gene="rapZ"
                     /locus_tag="VF362_00285"
     CDS             complement(375..1298)
                     /gene="rapZ"
                     /locus_tag="VF362_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX7589400.1"
                     /translation="MSSDADPETFPSGIPKVRVTLPAIPPEVLIVTGMSGAGRSRAAD
                     VIADEGWYVVDNLPPQMLAEMVTMVSREGYVRLAAVVDVRGGKFFADLEAVLGDLEAG
                     TVPARVMFLEASDEVLVRRFEQVRRPHPLQGDGTLLEAIRTERHALGPMRERADIVID
                     TSGTNAHELAERILAEIGDSEASSLQVNVQSFGFKNGAPEDADFLADVRFLDNPHWIP
                     ELRPLTGLDAPVRDHVLAAAGAGEFLDAYSAALDIALSRYRAHDKHFVTVAIGCTGGH
                     HRSVAFTEALAERLREFGYAVRASHRDRERE"
     gene            complement(1295..>2598)
                     /gene="uvrC"
                     /locus_tag="VF362_00290"
     CDS             complement(1295..>2598)
                     /gene="uvrC"
                     /locus_tag="VF362_00290"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX7589401.1"
                     /translation="LAEQVCAVLAGDAKGLVRDLTRQMDAASNEERFEAAARLRDRLA
                     ALTAVLERNTMVLAPGVDADVVGIEDDEYEAAAHVFHVRDGRITGQRAWVVDKPEPLG
                     TGELVTRLVQEIYDAPGAPAVPSEILVPSLPADAAALGDWLAGLRGGAVAVKVPARGP
                     KAELAETSHANAVETLAHHRLKRASDLTARSAALTALQEALGLPEAPLRIECYDISHT
                     GGTNQAASMVVFEDALPRTREYRHFSISDARDDTEAMAQVITRRFTRYLQEAGRPVEE
                     RDSSRFAYPPQLVVVDGGGPQVEAAHRALTALGVEGVSLCGLAKRLEEVWLPGDPFPV
                     ILPRGSEALYLLQRVRDEAHRFAIRQHRTRRSRAMTASQVDEIPGVGPARARALLRHF
                     GSLDKVREASVEQIALVPGIGRETAAAIARAVSGKGGMLEA"
BASE COUNT          408 a          905 c          911 g          374 t
ORIGIN      
        1 gcgaaggagc cgggcgaccc aatcgagccc ctcgaccacg tcgcccgtgc ggttccagag
       61 tccggcgatc agcaggttgc cgagcgagtg gccgtcgagg gggccgtcgg tgacgaaccg
      121 gtgttgaaga acgtctcgcc acatgtggcc ccactccgtg tcctcgcaca gcgcggacag
      181 ggccattcgc aggtcgccgg gaggggggac gcggagttca tccctgaggc gtcccgacga
      241 tcccccgtcg tcggccacgg tcacgatcgc ggtcaggttc tccgagagtt ggcgcaaggc
      301 actcagcgac gcgtagagcc cgtgaccacc gcccagcgcg acgataacca ggtccgacgc
      361 gccgcggtcg ccgctcactc gcgttcgcga tccctgtgcg aggcacgcac ggcgtacccg
      421 aattcgcgca gcctctcggc gagcgcttcg gtgaaggcga cggaacggtg atggccgccg
      481 gtgcagccga tcgcaaccgt cacgaagtgc ttgtcgtgcg cgcggtagcg gctaagcgcg
      541 atgtcgagtg cggccgagta ggcatcgagg aactcgccag ccccggcggc cgcgagcacg
      601 tggtcccgca cgggggcgtc gaggcccgtg agcgggcgga gctcggggat ccagtggggg
      661 ttgtcgagaa agcggacgtc cgcgaggaag tcggcatcct ccggcgcgcc gttcttgaag
      721 ccgaaggact gcacgttgac ctggagtgag gaggcctcgg agtccccgat ctccgccagg
      781 atccgttccg cgagctcgtg ggcattcgtg ccggaggtgt cgatgacgat gtcggcccgt
      841 tcccgcatcg gcccaagggc atgccgttcg gtccggatcg cctccaggag cgtcccgtcg
      901 ccctggaggg gatgtgggcg gcgaacctgc tcgaagcgac gaaccagaac ctcatccgat
      961 gcctcgagga acatcacgcg ggcggggacg gtaccggcct cgaggtcccc gagcaccgcc
     1021 tcaaggtccg cgaagaactt cccgccccgg acgtcgacga cggccgcgag gcgcacgtag
     1081 ccctcccgcg agaccatcgt caccatttcc gcgagcatct gaggcggcag gttgtcgacg
     1141 acataccagc cctcgtccgc gatgacgtcc gcggcgcggc tacgccccgc cccggacatc
     1201 ccggtaacga tcagaacctc gggcgggatc gcgggcaacg tcacacgcac cttcggaatc
     1261 ccggagggga aggtctcggg gtcggcgtcg gaactcatgc ctccagcatg ccacccttgc
     1321 cggagaccgc ccgagcgatc gccgccgccg tttcgcggcc gatccccggc acaagcgcga
     1381 tctgttcgac cgacgcttcc cgaaccttgt cgagggaacc gaagtgccga agaagcgccc
     1441 gcgcccgcgc cgggccgacc ccaggaatct cgtccacctg cgaggcggtc atcgcgcggg
     1501 aacgtcgggt gcggtgctgg cggatcgcga agcgatgcgc ctcgtcccgc acccgttgca
     1561 gcaggtagag cgcctccgaa ccgcgcggaa ggatgacggg aaacggatcg ccgggaagcc
     1621 acacctcttc cagccgcttc gcgagcccgc agagggacac cccctcaacg ccgagcgcgg
     1681 tgagcgcgcg atgcgcggcc tcgacctggg gcccaccccc gtcgacgacc acgagttgcg
     1741 gcggataggc gaacctgctg gagtccctct cctcgaccgg acgcccggcc tcctgaaggt
     1801 agcgggtgaa gcgtcgggtg atgacctggg ccatcgcttc cgtatcgtcc ctcgcgtccg
     1861 agatcgagaa gtggcggtat tcccttgtgc gcggcagggc atcctcgaac acaaccatgg
     1921 atgcggcctg gttggttccg cccgtgtggg aaatgtcgta gcactcgatg cgtagcggcg
     1981 cctccggtag cccgagggcc tcctggagcg ccgtcagcgc cgccgagcgg gcggtgagat
     2041 cggaggcccg cttcaggcga tggtgcgcga gggtctctac ggcgttcgcg tgcgacgtct
     2101 cggcaagttc ggccttgggg ccgcgcgcgg gaaccttgac cgcgacggcc ccacccctga
     2161 gtcccgcgag ccagtccccg agcgcggcgg cgtccgcggg aagcgagggg acaagaatct
     2221 ctgagggaac ggcgggggcg ccgggagcgt cgtagatctc ctgcacgagg cgcgtgacga
     2281 gttcccccgt ccccagcggc tccggcttgt ccaccaccca cgcgcgttgg cccgtgatcc
     2341 gcccgtcgcg gacgtgaaag acgtgcgccg cggcctcgta ctcgtcgtcc tcgatgccca
     2401 ccacgtcggc gtcgacgcca ggggcgagca ccatggtgtt gcgttcgagc accgccgtga
     2461 gggccgcgag ccggtcgcga agtcgcgcgg cggcctcaaa gcgttcctcg ttcgacgccg
     2521 cgtccatctg gcgggtgagg tcccgtacga ggcccttggc gtcgcccgcg agcaccgcgc
     2581 acacctgttc cgcgaggt
//
LOCUS       DASVKP010000014         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_497473, whole genome shotgun sequence.
ACCESSION   DASVKP010000014 DASVKP010000000
VERSION     DASVKP010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497473"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VF362_00295"
     CDS             complement(<1..385)
                     /locus_tag="VF362_00295"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009213061.1"
                     /note="catalyzes the phosphorylation and decarboxylation
                     of oxaloacetate to form phosphoenolpyruvate using GTP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX7589402.1"
                     /translation="MSTATSPAPAHAHPTIVDWVAKIAELTTPTAIHWCDGSPSERQG
                     LIDGMVELGTLTPLSPEKRPNSYLARSHPSDVARVEARTFICSEREEDAGPTNNWADP
                     KEMLQTLSDLFEGTMAGRTMYVVPFA"
     gene            661..1983
                     /gene="tyrS"
                     /locus_tag="VF362_00300"
     CDS             661..1983
                     /gene="tyrS"
                     /locus_tag="VF362_00300"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX7589403.1"
                     /translation="MTHILDELAWRGLISQTTGDDALRAAIDAGPITLYCGFDPTAPS
                     LHHGNLVQILTVRRFQDAGHKPLLLVGGATGLIGDPNMSGERTLNPVDVVHGWVERIR
                     AQIEPLMSFEGDNAAVMVNNYEWTKDLDAISFLRDVGKHFRMGTMLAKENVARRLASD
                     EGISYTEFSYQVLQGMDFLELYRRYGCTLQTGGSDQWGNLIAGTELTRKVEGVAVHGL
                     ATPLITKADGTKFGKTEAGAVWLAPDMMSPYAFYQFWLNSDDADVVNLLKVFTFRSRE
                     EIEALGRAVADEPFKREAQRALAEDVTALVHGSAALGAVVAASQAIFGRGELAALDAA
                     TLAGVADSVPSAEVAAGTSFVDALVAVGLVESRSEARRAIADGGAYLNNENVADPDFV
                     LTTSAAIAGGWAVVRRGKKTVGLVRIGGAAGAATRPGAAGFDSAPGTP"
     gene            2435..>2526
                     /locus_tag="VF362_00305"
     rRNA            2435..>2526
                     /locus_tag="VF362_00305"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          406 a          784 c          895 g          441 t
ORIGIN      
        1 tcgcgaacgg gacgacgtac atggtgcgcc cggccatcgt tccctcgaac agatccgaca
       61 gggtctggag catctccttg gggtctgccc agttgttcgt cggccctgcg tcttcctcgc
      121 gctcggagca gatgaaggtg cgcgcctcga cccgcgcgac gtcgctcggg tgggagcgtg
      181 cgaggtagct gttggggcgc ttctcggggc tgagtggcgt gagggtcccg agttcgacca
      241 tcccgtcgat gaggccttgg cgctcggagg gcgagccgtc gcaccagtgg atcgccgttg
      301 gggtggtgag ctcggcgatc ttggccaccc aatcgacgat ggtggggtgc gcgtgcgccg
      361 gcgctggcga agtggcggtg ctcaagtcag tcacggctgt ccttctgtgg tggtctgaag
      421 cgcctcaggt cttctgccgc ttctttgcgg gttgcggctc tcgggtgagg gtcgcgtggt
      481 gggcttgcac gacctcgtcg ggcggggtca tgccgaggct accgtggagg cgtcggttgt
      541 tccaccggtc gtcccggccg gcggtggcga gcgccgggtg acccgaccgc tggcgaccac
      601 ggcccaaccc ccctcggccg tcgcccgacc cgcccgtcgt gctggcagac taggcgcatc
      661 atgacgcaca tcctcgacga acttgcgtgg cgcgggctga tttcgcagac caccggtgac
      721 gacgcactcc gcgccgcgat cgacgccggg ccgatcaccc tatattgcgg gtttgacccc
      781 acggcgccga gccttcacca cgggaacctc gtgcagatcc tcaccgtgcg ccgatttcag
      841 gacgccggcc acaagccgct cttgctcgtc ggcggcgcaa cggggctcat cggcgatccg
      901 aacatgtcgg gggagcgcac cctgaatccc gtggatgttg tccatggttg ggtggaacgg
      961 attcgggcgc agatcgagcc cctgatgagc tttgagggcg acaacgccgc agtcatggtc
     1021 aacaactatg agtggacgaa ggacctcgac gccatcagct tccttcgtga cgttggcaag
     1081 cacttccgca tggggacgat gctcgcgaag gagaatgtcg cgcgaagact cgcgtcggac
     1141 gaggggattt cgtacaccga gttctcgtac caagtgctcc agggcatgga cttcctcgag
     1201 ctctaccggc ggtacggctg cacgcttcag acgggaggct ccgaccaatg gggcaacctc
     1261 atcgcgggaa ccgaactcac ccgcaaggtc gagggggtgg ccgtccacgg gctggcgact
     1321 cccctcatca ccaaggcgga cggaacgaag ttcggcaaga ccgaggcggg tgcggtgtgg
     1381 ctcgcgccgg acatgatgag cccctacgcc ttctaccagt tctggctcaa tagcgacgac
     1441 gcggacgtgg tgaacctgct caaagtcttc acgttccgct cgcgggagga gatcgaggcg
     1501 ctcggccgtg cggtggcgga cgagcccttc aagcgcgagg cgcagagggc gctcgcggag
     1561 gacgtaacgg cgctggtgca cggctccgcg gccctgggtg ccgttgtggc ggcctcgcag
     1621 gccatcttcg gccgtgggga gctcgcggcg ctcgacgcgg ccacgctggc gggcgtggcg
     1681 gattcggtcc cctccgccga ggtcgcggct ggcacgagct tcgtcgacgc gttggtcgcc
     1741 gtcgggctcg tcgagtcgcg ctccgaggcg cggcgcgcga tcgccgacgg gggcgcgtac
     1801 ctcaacaacg agaacgtcgc ggatcccgac ttcgtgctga ccacctcggc ggcgatcgcc
     1861 gggggctggg ccgtggtgcg gcggggcaag aagaccgtcg ggctggtgcg catcgggggc
     1921 gccgcggggg cggcgacacg cccgggagcc gcgggatttg actcggcccc aggaaccccg
     1981 taagttatcc ctcgctgcct gaacgggagc gctcgacgga ggactgaaga aggccacggc
     2041 gagaccctca ggacggcccc tcttgagttg gacccgatgg gtgctgtctg cgcccaaccg
     2101 gggtatgatg gagggcccgc gaaccctgcg aaccgccgac gaggtggttt gacagagggt
     2161 atcgggaaga ctaagtttgt agggttgccc tggcgccggt tgagagcccg gagttgggcg
     2221 cgctcgatcc ttgagaactc aacagcgtgc cacatagtcg atgccaagaa cccggcgtcg
     2281 tcagcccagc ctcggccggg aactgcgacg acggtttact ttggtaaaga aacaaatgtc
     2341 agtgacattt tgttcatttg ctagagattg aacggtcctt cggggccact tcgtaaagtc
     2401 ggtcacccgc gcctagcgcg gataccaaaa gacattcacg gagagtttga tcctggctca
     2461 ggacgaacgc tggcggcgtg cttaacacat gcaagtcgaa cggtgacctc ggagcttgct
     2521 ccgggc
//
LOCUS       DASVKP010000015         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_333180, whole genome shotgun sequence.
ACCESSION   DASVKP010000015 DASVKP010000000
VERSION     DASVKP010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333180"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..587)
                     /locus_tag="VF362_00310"
     CDS             complement(54..587)
                     /locus_tag="VF362_00310"
                     /inference="COORDINATES: protein motif:HMM:NF037150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX7589404.1"
                     /translation="MRAALALFSAHGYEATSMRAIAKDAGVDPSLPRHYFPSKSALFV
                     ESLGPLDQLEGNIGLITSGPVDRLGERMMGAFLAVWDSPELNARMRILVQSAAGTPEI
                     AEIARGLLFERLFLRVATSVGPEDAHARAAAALSQVIGLVVTRYILRVEPVASAPREE
                     IIRRFAPNVQRLITGTT"
     gene            complement(655..>1791)
                     /locus_tag="VF362_00315"
     CDS             complement(655..>1791)
                     /locus_tag="VF362_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589405.1"
                     /translation="PPPTTTGPPVPPPPSSWPQSPAPASGPFSFPTTGSVPRPPASGV
                     PVPPPPLPWPQSPAPASAPAIPQFVLPASGSLPAASGSLPAAKEGRRGLFGIAWLGGR
                     KAGKSEDAVPASSGGPAGDDGDGDEPSNRIAAFFERASVLVVLGLFAFVGIGSGVYLL
                     FFRPEPVVMPPSVHTIPRPTPTFEPLVVTDATDFLAALPKMDLAYGLRSAESTVMRPV
                     TVWPSRFAEEWLLTYDAGAGGTMTVQAVQHYKLEVATSTYQHLLDVATAEALAARSAG
                     PSPSPGASPAVSPSPAPSTSANPSPTDAPGMVMGTVYVDKVEVGKSFKVVKDVTESVA
                     DPAGGDPTEVTRKVAVVTWQNGTGVFIMTADPAVIDDLFLGYGL"
BASE COUNT          298 a          565 c          669 g          259 t
ORIGIN      
        1 tgtttcccca tgtgatgaat aatcccttct cgtcgtggag ttgtcaagcg cccctaggtc
       61 gttcccgtga tgagtcgctg cacattgggc gcgaatcgcc gaatgatctc ctcgcggggg
      121 gcggaggcga cgggctcgac ccgcaggatg tagcgcgtga cgacgaggcc gatcacctgc
      181 gagagcgcgg cggccgcgcg ggcgtgggca tcctcggggc caacagacgt ggccacacgc
      241 aggaagaggc gctcgaaaag gagtcctctg gcgatctcgg cgatctctgg tgtgccggcc
      301 gcgctctgca ccaggatcct catgcgcgcg ttcagctccg gcgagtccca aacggccagg
      361 aaggcgccca tcattctctc gcccagacgg tcgaccgggc cggacgtgat cagcccaatg
      421 ttgccttcga gctgatcgag ggggcccagt gattccacga agagtgccga cttggagggg
      481 aagtagtggc ggggaagcga cggatcgacc cccgcgtcct tcgcgatggc acgcatggac
      541 gtcgcctcat agccgtgggc gctgaagagc gcgagcgcgg cgcgcacgat gtccccgcgc
      601 gtgtccgcgt gtgccccgcg cgggccccga ggccgtgacg cgagggcgga catcctagag
      661 cccgtacccg aggaacaggt cgtcgatcac cgccggatcg gccgtcatga tgaagacccc
      721 cgttccgttc tgccacgtca cgaccgcgac cttgcgggtg acctcggtgg gatcgcctcc
      781 ggcagggtcg gcgacggact cggtgacgtc cttgacgacc ttgaagctct tgcccacctc
      841 gaccttgtcg acatagacgg tgcccatcac catgccgggt gcgtcggtcg gcgatgggtt
      901 ggcggaggtc gacggggcgg gtgagggtga cacggcgggt gaggcgccgg gcgagggcga
      961 aggtcctgcg gaccgggccg ccagcgcctc cgcggtcgca acatcgagca gatgctgata
     1021 cgtggaggtt gcgacctcga gtttgtagtg ctggacggcc tgaacggtca tggtgccgcc
     1081 ggcgccggcg tcgtaggtca ggagccactc ctcggcgaac cgcgagggcc ataccgtcac
     1141 ggggcgcatg acggtggact ctgcgctcct caggccgtac gccagatcca tctttggaag
     1201 ggcggcgagg aagtccgtcg cgtcggtcac cacgagcggc tcgaacgtcg gcgtcggtct
     1261 cgggatggtg tggacggaag gcggcatcac caccggctcg ggacggaaga acaacaggta
     1321 gacgccggat ccgatgccga cgaaggcgaa gaggccaaga accacgagga cggaggcccg
     1381 ttcgaagaac gcggcgatac ggttcgacgg ctcgtcgccg tcgccatcat ctcccgccgg
     1441 accgcccgac gaggccggta ccgcgtcctc gctcttgcca gccttgcggc cacccagcca
     1501 cgcgattccg aagagcccgc gcctgccctc tttcgcggcg ggaagggagc cactcgcggc
     1561 gggaagggag ccgctcgcag gcaggacgaa ctggggaatc gccggcgccg acgctggcgc
     1621 tggggattgc ggccacggga ggggaggagg aggaacaggc acccccgacg ccgggggccg
     1681 cggcaccgag cccgtggtag ggaaggagaa gggtcccgac gctggcgctg gggattgcgg
     1741 ccacgaggac ggggggggag gaacgggtgg gcccgtcgtt gtcggcggcg g
//
LOCUS       DASVKP010000016         6352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1237393, whole genome shotgun sequence.
ACCESSION   DASVKP010000016 DASVKP010000000
VERSION     DASVKP010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237393"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VF362_00320"
     CDS             complement(<1..381)
                     /locus_tag="VF362_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589406.1"
                     /translation="MSETVEGTVRRMWRIGVIVGMIGVAGALAGCSASADSYNGGDAA
                     LAGDAAPAGGDAAKDVVAAAEAAPPSTADRSVVITGAMYMTVEDPIAAADRTVGIVLN
                     AGGRIDARSEAAPGERDGGSASLTL"
     gene            613..852
                     /locus_tag="VF362_00325"
     CDS             613..852
                     /locus_tag="VF362_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7589407.1"
                     /translation="MADGPAPRTTFLTVAEVADMLRVSRMTVYRWVHAGDLPAVRFGR
                     SFRIPQQAVDVFMQNAALVGGYPAEDDGRAAGAVG"
     gene            931..1029
                     /locus_tag="VF362_00330"
     CDS             931..1029
                     /locus_tag="VF362_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HEX7589408.1"
                     /translation="MGSVIKKRRKRMSKKKHRKLLRKTRHQRRNKK"
     gene            complement(1054..1899)
                     /locus_tag="VF362_00335"
     CDS             complement(1054..1899)
                     /locus_tag="VF362_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX7589409.1"
                     /translation="MTPTDRELANDGQGRIVAFFDVDNTIIRGASPYHIARGLRRRGF
                     FTRREVIGFAWEQAKYLVFGETKRQMNEVREEALSIIKGWSVAEMTSIAEDVYDEVLA
                     LRIFPGTKKILDTHVQQGHEVWLVTASPVEVGRMIARRLGATGALGTIAQHERGHYTG
                     ALVGDLMHGENKAHAARQLARERGLDLAHSYGYGDSLNDVPLLETVGNPVAINPDLRL
                     RRYAKRRGWDLRDFRGRKGNGRRSIVARTTAGTVWALLSVLRILKRTVVGVLRGLVHS
                     LVRRR"
     gene            2029..2649
                     /locus_tag="VF362_00340"
     CDS             2029..2649
                     /locus_tag="VF362_00340"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7589410.1"
                     /translation="MTVVHLVRHGHVHNPERVLYGRLPEFRLSEVGERMAEATARSFM
                     ESGRDIARLVSSPLLRAQQTAAVIGAALGLLVETDDRLIEADNVWEGRRIRPIARLAL
                     DPRTLWRVRNPWRPSWGEGYAGQRDRMMAAVLDAAATEPNRETVLVSHQLPIWVTRLA
                     AEGRALAHNPSARQCALASVTSLIVEDGAVIGVEYAEPAASVEVPR"
     gene            2665..3798
                     /locus_tag="VF362_00345"
     CDS             2665..3798
                     /locus_tag="VF362_00345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7589411.1"
                     /translation="MKIALVLDDSLDRPDGVQQYVLSVGRYLSSVGHDVHYVCSSATR
                     TDLGQQLHSLVGNFHVSFNGNGLSIPRVASRRVLREFVERERFDVFHVQTPHSPLFSA
                     RLVRQARRVQGRAVTIVGTFHILPNGRIAALGTRLLGVVLRHNLRAFDRFCAVSAPAA
                     EFARRMFAIDPRVIGIPIPVDLLAAAGAKGRASRVGSTRLHLAFLGRLVPRKGSLEFV
                     EAVAALEPQVRDRVRVTIGGRGPLAADIERAIETHTLQSIVEMAGFVPEDDKAAFYGA
                     ADIAVFPATGGESFGIVLTEAMASGSGVVVGGDNPGYRSVLGDSPDVLVDPGDTAAFA
                     AKLARLIADSSLRASIRADQEARVREFDVAVVGPKIEALYAAP"
     gene            3894..4241
                     /locus_tag="VF362_00350"
     CDS             3894..4241
                     /locus_tag="VF362_00350"
                     /inference="COORDINATES: protein motif:HMM:NF025378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4229 domain-containing protein"
                     /protein_id="HEX7589412.1"
                     /translation="MHVLFYWLARAALMFAVFVALWGVGWKPWFAVVGATVVAWLISY
                     AMFGSMHDAAARQMERWVSRRFVSVAGDAEAKVEGAPNLGATPRAASPAKPRAASPAR
                     PKPQPKVAARRSS"
     gene            complement(4238..5101)
                     /locus_tag="VF362_00355"
     CDS             complement(4238..5101)
                     /locus_tag="VF362_00355"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEX7589413.1"
                     /translation="MRDWVRGARPKTLASAIAPVAVGSASAAVLGAFRPGIAALALGV
                     ALALQVAVNFANDYSDGVRGTDANRVGPERLVASGKVAPSRVRAAALAAFAVGSLLGL
                     AMTLISREWWLLAVGAAAILAAWTYTGSSRPYGYRGWGEVSVFIFFGLVATLGTMAAQ
                     AGRVTWWAVVAATGVGLYSVAMLLVNNIRDLAPDRLTGKRTLAVRLGDAAARRLFAAS
                     VTLPIVASGAIALARPWALLTLVLAAPAVMLAAAMRAGLAGRPMTAAFAATSAIGLAY
                     GVLLALGIAIG"
     gene            complement(5094..6164)
                     /locus_tag="VF362_00360"
     CDS             complement(5094..6164)
                     /locus_tag="VF362_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7589414.1"
                     /translation="MTYASLAQDPDAALRAALAGEVAGVAIATSGSTGKPRLAYVSGA
                     ALRAPAAATHERLGGPGHWLLALPPDRVAGAQVLVRSALGGHPAVRLRPGSFDPRAFA
                     EAALATRSDTAASVPLYVSLVPTQLHRIADDPAAVLALEVFDAILVGGAALRRDVAPP
                     NAIETYGSTETAGGCVYDGRPLPGVEVELEGDGRVLISGPILFDGYADGGDDGTVFRD
                     GKRWLRMPDVGDWADARLAVLGRADDVIVTGAFKVHPATVERAIRILPEIADVVVVAA
                     PDEEWGERIVALVSLADRRATITTDALRTEVADTLPRHALPRQVIVVEKLPHLDSGKI
                     DRRAARALARASNGGPRARDDA"
BASE COUNT          951 a         2204 c         2225 g          972 t
ORIGIN      
        1 cagcgtgagc gaggctgagc cgccgtcgcg ctcaccgggc gcggcctcgc tgcgcgcgtc
       61 gatgcgtccg cccgcgttca gtacgatgcc cactgtccgg tcggcggccg cgatcgggtc
      121 ctcgaccgtc atgtacatgg cgcccgtgat gactacggaa cggtcggcgg tcgacggcgg
      181 ggcggcctcg gcggccgcca caacgtcctt ggccgcgtcg cctccggcgg gtgccgcatc
      241 gccggcgagt gcggcatcgc cgccgttgta cgaatcggcg gacgcggaac agcccgcaag
      301 cgcccccgcg acgccgatca ttccgacgat gaccccaatg cgccacatgc ggcgcaccgt
      361 cccctcaacc gtttcgctca tggtgttcat agtagggaga cgcttggcga agcgcgagcg
      421 ttgggtgcgc cctccctaag ttgggccgcc gaaggggcgg gcttgcgcag gacgtaaacg
      481 aacgaggcca agcaaggccg ccccttcggc ggcccgcatc ctcccttcac atccgtcaca
      541 acagccccta cagtggggaa cacgttgcgc cgatagtgac ggtgaacgtc gtctaaggat
      601 gtgaaacaga ccatggcaga cggccctgcg cctcgcacga ccttcctcac cgtcgcggaa
      661 gtcgccgaca tgcttcgcgt gtcgcgcatg acggtgtacc gctgggtcca cgcgggcgat
      721 ctgcctgccg tccgattcgg gcgctcgttc cgcattcctc agcaggccgt tgacgtcttc
      781 atgcagaacg ccgcgctcgt cgggggctac ccggccgagg acgacgggcg cgctgcgggc
      841 gcggtaggct agggagccgt ctcgcgggcg cagtaggttc tcagtcgtcg cgagcaagcc
      901 agaatcgtcc agccctaaga atgaggtccc gtgggttccg tcattaagaa gcgtcgcaag
      961 cgcatgtcga agaagaagca ccgcaagctc cttcgcaaga cgcgccacca gcgccgcaac
     1021 aagaagtagg gaatcccgac gcgcctcggc gcgctatcgg cgtcgtacga ggctgtgtac
     1081 caggccacgc aacacgccga cgacggtccg cttcagaatg cgaagcacgc tgagtagcgc
     1141 ccacaccgtc cctgctgtgg ttctcgcaac gatgctgcgg cgtccgttgc ccttgcgacc
     1201 gcggaagtcc ctgaggtccc aacctcgcct cttcgcatag cggcgcaggc gcaaatcggg
     1261 gttgatcgcc acggggttgc ccacggtttc tagcaacggg acgtcgttga gcgaatcgcc
     1321 gtagccgtag gagtgcgcga ggtcgaggcc ccgttcacgc gcaagttggc gcgcggcgtg
     1381 ggccttgttc tcgccgtgca tgagatcgcc gacgagcgcg cccgtgtaat ggccgcgttc
     1441 gtgctgggcg atggtgccga gcgctccggt cgcgccgagc cgccgcgcga tcatgcgccc
     1501 cacctcgacg ggggaagcgg tcacgagcca cacctcgtgt ccctgctgaa cgtgggtgtc
     1561 gaggatcttc ttggttccgg ggaagatgcg gagcgcgaga acctcgtcgt agacgtcctc
     1621 ggcgatcgag gtcatctccg cgacgctcca ccccttgatg atcgaaaggg cctcttcgcg
     1681 cacctcgttc atttgtcgct tggtctcgcc aaacacgagg tacttggcct gctcccacgc
     1741 gaagccgatc acctccctgc gggtgaagaa tccgcgtcgg cgcaggccgc gggcgatgtg
     1801 ataggggctg gccccgcgga tgatcgtgtt atcgacgtcg aagaaggcga cgatgcgtcc
     1861 ttgcccatcg ttcgccagtt cgcggtcggt cggcgtcacg ggttccaccc tacctaccgg
     1921 ctcaccgcgt ccggtacgtg gggtcgcccc aaggcctcga gcggcggggc gatcagaagc
     1981 ggtccccctc tggtgagccg gacctcccag gtttgggaca ctagagccat gaccgtcgtc
     2041 cacctcgtcc gccacggcca cgtccacaac cccgagcgcg tcctctacgg gcgcctgccc
     2101 gagtttcggc tgtccgaggt gggggagcgt atggccgagg cgaccgctcg ttctttcatg
     2161 gagtccggcc gcgacatcgc gaggctcgtc tcctcccccc tcctgcgcgc gcaacagacg
     2221 gccgcggtga tcggtgccgc gctcgggctt ctcgtggaga cggatgaccg cctgatcgag
     2281 gcagacaacg tctgggaggg gaggcgcatc cgccccatcg cccggctcgc cctggatccg
     2341 cggaccctct ggcgcgtccg caacccgtgg cggccctcat ggggagaggg gtacgcgggc
     2401 cagcgcgaca ggatgatggc cgcggtgctg gatgctgcgg cgaccgagcc gaatcgcgag
     2461 accgtcctgg tgagccacca acttcccatt tgggtgacga ggcttgcggc ggaggggcgc
     2521 gcgctcgcgc acaacccgtc ggcccgccag tgcgcgctcg cgtccgtcac gagcctcatc
     2581 gtggaagacg gggcggtcat tggggtcgag tacgccgagc ccgccgcctc ggtcgaggtc
     2641 ccgcggtgag gggggtctag acgcatgaag atcgccctcg ttcttgacga cagcctcgat
     2701 cggcccgacg gggtgcagca gtacgtgctg agcgtgggga ggtacctctc gtccgtaggg
     2761 cacgacgttc actacgtgtg ctcgtcggcc acgcggaccg accttgggca gcaactgcac
     2821 tcgctcgtcg gcaacttcca cgtgagcttc aacggcaacg ggctgagcat ccctcgcgtc
     2881 gcgtccagac gcgtcctgcg ggagttcgtt gagcgtgagc gcttcgacgt cttccatgtc
     2941 cagacgcccc attcgccgct cttctccgcg cgcctcgttc gccaggcgcg cagggtgcag
     3001 ggtcgagccg tgacgatcgt cggcacgttc catatcctcc cgaacgggcg gatcgcggcc
     3061 ctcggcaccc ggctcctggg ggtggtgctt cgccacaacc tgcgtgcctt cgatcgcttc
     3121 tgcgcggtct cggctccggc cgcagagttc gcgcgccgca tgttcgccat cgacccgagg
     3181 gtgatcggca tccccatccc cgtcgacctc ttggcggccg ccggagcgaa gggtcgggcc
     3241 tcgcgcgtcg gctcgacgcg cctccacctt gcgttccttg gccgactggt cccgcgcaag
     3301 ggctccctcg aattcgtcga ggccgtggcg gcgctggaac cccaggtgcg cgatcgcgtg
     3361 cgcgtcacca tcggggggcg cgggccgctc gccgccgaca tcgagcgggc gatcgagact
     3421 cacacccttc agtcgatcgt cgagatggcc gggttcgttc ccgaggacga caaggcggcc
     3481 ttctacggcg cggcggacat cgcggtcttc cccgcgaccg gaggagagag tttcgggatc
     3541 gtcctcaccg aggccatggc cagcggatcg ggagtagtcg tcgggggcga caatcccggc
     3601 tatcggagcg tgctcggcga cagccccgac gttctcgtcg acccggggga caccgccgcc
     3661 ttcgccgcga aactcgcgcg cctcatagcg gactcttctc tccgtgcctc cattcgcgcg
     3721 gaccaagagg cgcgcgtgcg cgaattcgac gtcgcggtcg tgggacccaa gatcgaggcc
     3781 ctctacgcgg caccctagtc gccgaggttc cggcgcctct cccgttcgcg gagccacgcg
     3841 aggtagtcgg cgtcgtcggg ggcgagagca cgcggccacg gtacgatggc gcggtgcacg
     3901 tcctcttcta ctggctcgcg cgggccgcgc tgatgttcgc cgtgttcgtt gccctgtggg
     3961 gagtcgggtg gaagccctgg ttcgccgtcg tgggtgcgac cgtagtcgct tggctgatct
     4021 cctacgcgat gttcgggtcg atgcacgacg cggcggcccg tcagatggag cgatgggtgt
     4081 cgcgtcgctt cgtcagcgtt gcgggggatg ccgaggcgaa ggtcgagggc gcgccgaatc
     4141 tcggcgctac acccagggcc gcctcgcccg ccaagcccag ggccgcctcg cccgccaggc
     4201 ccaagccgca accgaaggtg gcggcccggc gatccagcta gccgatcgcg ataccgagcg
     4261 ctagcaagac tccgtacgcg agcccgatcg cgctggtcgc cgcgaatgcg gcggtcatcg
     4321 gccttccggc gagcccggcc ctcatcgcgg cggcgagcat gaccgccgga gcggcgagga
     4381 cgagcgtgag gagcgcccac gggcgcgcga gcgcgatcgc cccgctcgcg acgatgggaa
     4441 gagtcacgga tgcggcgaag aggcgccgcg ccgcggcgtc tccgaggcgg acggcgaggg
     4501 tgcgcttccc ggtcaagcga tcgggtgcca ggtcgcggat gttgttgacg agaagcatcg
     4561 ccaccgaata gaggcccact cccgtcgcgg cgacgacggc ccaccaggtg acgcggccag
     4621 cttgggccgc catcgttcca agggtcgcaa caagtccgaa gaagatgaag acgctgacct
     4681 caccccatcc gcgatatccg taggggcggg acgagcccgt gtaggtccag gccgcgagga
     4741 tcgcggcggc gccgaccgcg aggagccacc actcgcgcga gatgagcgtc atcgcgaggc
     4801 cgagcaggga gccgacggcg aaggcggcca gggcggcggc tcgaacgcga gagggcgcca
     4861 ccttgccgga ggcaacgaga cgctccggcc ccacgcggtt cgcgtcggtt cctcggaccc
     4921 cgtccgagta gtcgttggcg aagttcaccg cgacctgcag ggcgagcgcg acgccgaggg
     4981 cgagcgcggc gattcccggc cggaacgcgc caagcactgc cgccgacgcg gagccgaccg
     5041 ccacgggcgc gatcgccgat gcaagcgtct tggggcgtgc cccgcgaacc cagtcacgca
     5101 tcgtcgcggg cacggggacc tccgttcgat gcgcgggcaa gggcgcgggc ggccctccgg
     5161 tcgatcttgc cagagtccag gtgcggaagc ttctccacga cgatcacctg ccggggcaag
     5221 gcatggcgcg gcagcgtgtc cgcgacttcg gttcgcaaag cgtctgtggt gatggtcgct
     5281 cgccggtcgg cgagggaaac gagcgcgacg atccgctccc cccactcctc gtccggcgcg
     5341 gcaacgacga cgacgtcggc gatctccggc aggatgcgga tcgctcgctc gacggtcgcg
     5401 gggtgcacct tgaacgcccc ggtgacgatg acgtcatccg cgcggcccag caccgcgagc
     5461 ctggcgtcgg cccaatcgcc gacgtccggc atgcggagcc agcgcttgcc gtcccgaaag
     5521 accgttccgt cgtcgccgcc atcggcatag ccatcgaaga ggatggggcc cgagatgagg
     5581 acccggccgt caccttccaa ctcgacctcg acgcccggca acggacggcc gtcgtagacg
     5641 catcccccgg cggtctcggt ggagccgtag gtctcgatcg cgttgggcgg ggcgacgtcg
     5701 cgtcggagcg cagcgccccc gacaaggatg gcgtcgaaga cctcgagcgc aagcacggcg
     5761 gcgggatcgt cggcgatcct gtggagttgg gttggcacca gggagacgta gagcggcacg
     5821 ctcgcggccg tgtccgacct ggttgcgagt gcggcctcgg cgaacgcccg cggatcgaaa
     5881 ctccccggcc gcaggcgtac ggcggggtgt ccgccaagcg cggagcggac gagtacctgg
     5941 gcccccgcaa cgcggtcggg cgggagggcc agaagccagt ggcccggacc gccgaggcgt
     6001 tcgtgcgttg ccgcggcggg ggcgcgcagc gcggcgccgc tgacgtatgc gagccgcggc
     6061 ttgcccgtcg acccagaggt ggcgatggcg acgccagcga cctcgcccgc cagggccgcc
     6121 ctgagagccg catcgggatc ctgggcgagg ctcgcataag tcaccctcca agcctatgcg
     6181 tgcctagact gtcggctatg aaccgcaccg ccagccgcgt cgggggggcc gcgatcgccc
     6241 tggcggtcgt cgtcctcgcc gcgtgcgcgc cgcaggaggt ctatcagccc gccgtcacgc
     6301 agaccctcct tcccgttgac catcgctctg cggtgccgtc gccgccggtg ga
//
LOCUS       DASVKP010000017         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_251872, whole genome shotgun sequence.
ACCESSION   DASVKP010000017 DASVKP010000000
VERSION     DASVKP010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251872"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..124
                     /locus_tag="VF362_00365"
     CDS             <1..124
                     /locus_tag="VF362_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX7589415.1"
                     /translation="EARAATYPAWLHHYNHHRPHTGIGGKSPIERVHNVPRNYT"
     gene            complement(121..984)
                     /locus_tag="VF362_00370"
     CDS             complement(121..984)
                     /locus_tag="VF362_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX7589416.1"
                     /translation="MTSRVMVVTDSAASLPEALIKRWDIAVVPLEVTIDDVVYREATD
                     RDSDRVLDALEAGRKVSTSQPSVGAFEGVYRALAERGADRIVSIHISGKISGTVNGAR
                     LAAASSPVPVLVVDTGSVGMGAGWPALAAAAKAKNGASADEVAQEARRVAASASMLLT
                     VDSLEYLRRGGRVPGTIALLGDALNVRPVLGVSEGEIVVLERVRTTPRARAAVVARAE
                     AAIAELPNPGLAIMSLRAQAFADDATAVLELKYPHLAKLVRTPVTSVLAVHGGPGSFA
                     AAVADLPIELR"
     gene            complement(1013..>2951)
                     /locus_tag="VF362_00375"
     CDS             complement(1013..>2951)
                     /locus_tag="VF362_00375"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010078609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX7589417.1"
                     /translation="TPVTKKKLTQWYFKVTQYADELLDGLETLRGAWQDRVIAMQRNW
                     IGRSSGADVQFQTVGRNEPISIYTTRPDTLFGATFMVVAADSDLAAELASGAGDDVKA
                     AFAAYVDDVKAATEIERLATDRPKTGVFLERYAINPVNGERLPIWASDYVLADYGHGA
                     IMAVPAHDQRDLDFARAMGLPVRVVVDCRDEAGDPLPDPAESGIATTGDGVVVNSPPI
                     DGLAKAEAIATITAILEKDGVGHAAKNYRLRDWLISRQRYWGTPIPIVHCDDCGEVAV
                     PDDQLPVRLPDSAGLDLSPKGQSPLAAATEWVSTTCPKCGKPALRDTDTMDTFVDSSW
                     YYLRYLSPNETDGPFDLAEARRWAPVDQYVGGVTHAILHLLYARFFSKALRDMGLVDY
                     DEPFTRLLNQGMVGMDGSAMSKSRGNLVRLSEQLDQFGVDAVRLTMVFAGPPEDDIDW
                     ADVSPDGAKRFLARAWRLARDVTSAPGSDPTAGNATLRAVTHKTLHECEGLVEGLRFN
                     VAVARTMELVNVARKAIDSGPGAADPAVREAAEAVAILLSLVAPYCAEDMWSMLGHEN
                     VARSLWPAVDPALLVEDTVTCIVQVMGKVRDRIEVPPSIGEAELRELALATAGAQRAI
                     GDGSIRTVIVRPPTLVNVVIG"
BASE COUNT          455 a         1039 c         1022 g          435 t
ORIGIN      
        1 cgaagcccga gcagccacct atccagcctg gctccatcac tacaatcacc acagacccca
       61 caccggaatc ggcggcaagt cacccatcga acgcgttcac aacgtcccta ggaactacac
      121 ctagcgcagt tcgatcggga ggtccgcgac cgcggcggcg aaggatccgg gtccgccgtg
      181 aaccgcgagc accgaggtca cgggggtccg caccagcttc gcaaggtgcg ggtacttcaa
      241 ctcgagtacc gcggtggcat cgtcggcgaa cgcctgcgcc ctgaggctca tgatcgccag
      301 acccgggttg ggcaactccg cgatcgccgc ctcggcgcgg gcgacaaccg ccgccctcgc
      361 gcggggcgtc gtccgaaccc gttcgaggac cacgatctcg ccctcgctca ccccgaggac
      421 gggccgcacg ttgagggcgt cgccgaggag ggcgatcgtc cccggcacgc gcccgccgcg
      481 cctcaggtac tcgaggctgt cgacggtaag gagcatggag gccgacgccg cgaccctgcg
      541 cgcctcctgg gccacctcgt cggcgcttgc cccgttcttc gccttcgcgg cggccgcgag
      601 ggcgggccac cccgcgccca tgcccacgct ccccgtgtcg accacgagaa ccggaacggg
      661 cgaggacgcg gccgcgaggc gggccccgtt gaccgtgcca gagatcttgc cggaaatgtg
      721 gatggacacg atcctgtcgg ccccgcgttc ggcgagggcc ctgtagacgc cctcgaaggc
      781 accgacgctc ggctgcgacg tggagacctt ccgccccgcc tcgagcgcgt cgagaacccg
      841 gtcggagtcg cggtccgtcg cctcgcggta cacgacgtcg tcgatggtca cctcgagggg
      901 aacgaccgcg atgtcccacc gcttgatcag cgcctcgggc agcgaggccg ccgagtcggt
      961 caccaccatc actcgagagg tcaccccctc agcgtatggc aggaagcgtc ccctatccga
     1021 tgacgacgtt gacgagggtg ggcggcctga cgatcacggt ccggatcgag ccgtcgccga
     1081 tcgcgcgctg cgcccccgcg gtcgcgaggg cgagttcccg tagttcggcc tcgccgatgg
     1141 acggaggaac ctcgatgcgg tcacgcacct tgcccatcac ctgcacgatg cacgtgaccg
     1201 tgtcctcgac gagaagcgcc gggtccaccg cgggccagag agagcgcgcg acgttctcgt
     1261 ggccgagcat ggaccacatg tcctcggcgc agtagggcgc gacgaggctt agcaggatcg
     1321 cgacggcctc cgccgcctcg cgaacggcgg gatccgcggc cccaggcccg gagtcgatgg
     1381 cctttcgggc aacgttcacg agctccatcg ttcgagcgac cgcgacgttg aagcgcaggc
     1441 cctccaccag gccctcgcac tcgtggagcg tcttgtgggt gaccgcgcgg agggtcgcgt
     1501 tgcccgccgt cggatccgac cccggcgcgc tcgtcacgtc gcgcgctagg cgccacgcgc
     1561 gcgccaggaa tcgcttggcg ccatcgggcg agacgtcggc ccagtcgatg tcgtcctccg
     1621 gaggcccggc gaacaccatc gtcagccgca cggcgtcgac gccgaactgg tcgagttgct
     1681 ccgacagccg cacgaggttg ccgcgcgact tggacatcgc cgagccgtcc atgccgacca
     1741 tcccctggtt cagcaggcga gtgaacggct cgtcgtagtc cacgagcccc atgtcgcgca
     1801 gcgccttgga gaagaagcgc gcgtacagga ggtggaggat cgcgtgcgtc actcccccga
     1861 cgtactggtc gacgggagcc cagcgcctcg cctcggcgag gtcgaacggc ccgtccgtct
     1921 cgttcggaga gaggtagcgc aggtagtacc aggacgagtc gacgaacgtg tccatcgtgt
     1981 ccgtgtcgcg cagcgcgggc ttgccgcact tcgggcaggt ggtcgagacc cactccgtcg
     2041 cggcggcgag cggcgactga cccttgggcg agaggtcgag ccccgcgctg tccgggaggc
     2101 gcacggggag ctggtcgtcg ggaacggcga cctcgccgca gtcgtcgcag tggacgatcg
     2161 ggatgggcgt accccagtac cgctgtcgag agatgagcca gtcgcgcagg cggtagttct
     2221 tggccgcgtg gcccaccccg tccttctcaa ggatggcggt gatcgtcgcg atcgcctccg
     2281 ccttggcgag cccgtcaatc ggcggggaat tgacgacgac gccatcgccc gtcgtcgcaa
     2341 tgccggactc ggccgggtcg ggaaggggat cgcccgcctc gtcgcggcaa tccaccacga
     2401 cgcggacggg aagccccatg gcgcgcgcga agtcgaggtc gcgctggtcg tgcgcgggga
     2461 cggccatgat cgcaccgtgg ccgtagtcgg cgaggacgta gtccgacgcc cagatcggga
     2521 gcctctcgcc gttcacgggg ttgatcgcgt agcgctcgag gaacacgccc gtcttggggc
     2581 ggtccgtggc gaggcgctcg atttcggtgg ccgccttgac gtcgtcgacg tacgccgcga
     2641 acgcggcctt gacgtcgtcc ccggcgcccg aggccagctc ggccgcgagg tcgctgtccg
     2701 ccgcgacgac catgaaggtc gctccgaaga gcgtgtccgg acgcgtcgtg tagatgctga
     2761 tcggctcgtt gcggcccacg gtctggaact ggacgtccgc gcccgaggag cgtccgatcc
     2821 agttgcgctg catcgcgatg acgcggtcct gccacgcacc gcggagcgtc tcgagcccat
     2881 ccaggagctc gtcggcatac tgcgtgacct tgaagtacca ctgggtgagc ttcttcttgg
     2941 tgacgggagt g
//
LOCUS       DASVKP010000018         3414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1403343, whole genome shotgun sequence.
ACCESSION   DASVKP010000018 DASVKP010000000
VERSION     DASVKP010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403343"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2793)
                     /gene="carB"
                     /locus_tag="VF362_00380"
     CDS             complement(<1..2793)
                     /gene="carB"
                     /locus_tag="VF362_00380"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX7589418.1"
                     /translation="MPRRDDIRSVMVIGSGPIVIGQACEFDYSGTQACRVLKAEGIRV
                     ILVNSNPATIMTDPEFADATYIEPITTEVIEAIIAKERPDALLPTLGGQTALNAAVAL
                     VEAGILEKYNVELIGAKIDSIHMAEDREQFKHVVARCGAESARSFICHTMDECLAAAD
                     ALTYPVVVRPSFTMGGLGSGFAYTPDDLRRIAGAGLHYSPTTEVLLEESLLGWKEYEL
                     ELMRDRHDNVVVVCSIENIDPVGVHTGDSVTVAPALTLTDREYQRLRDIGIAVLREVG
                     VDTGGCNIQFAVQPSTGRVIVIEMNPRVPRSSALASKATGFPIAKIAARLAIGYTLDE
                     IPNDITGSTPASFEPTLDYVVVKVPRFAFEKFPAADPVLTTTMKSVGEAMSLGRSFTE
                     ALGKALRSIDKKGVNFHWRGEVPAGSALDDLIATTATPTEWRLLQVQQALRAAIADPG
                     VLDRVFDATRIDPWFLDQILLMNEVAAEVAAAPELGATLLRRAKSHGLADVQIAEIRG
                     TTEGEVNALRHALGVTPVYKTVDTCAAEFEASTPYHYSTYDSENEVHPREREAVIILG
                     SGPNRIGQGIEFDYSCVHAAMALKDTYETIMINCNPETVSTDYDTSDRLYFEPLTFED
                     VMAVFEAEKAAGPIKGMIVQLGGQTPLSLAQRLADAGVPILGTSPEAINLAEDRGEFG
                     KVLATAGLPAPAFGTAFGIDEAIVIAERIGYPVLVRPSYVLGGRGMEIVYDRAQLEDY
                     GIRSVDVGDHATAAPLLVDRFLDDAIEIDVDALFDGVEMFLGGVMEHIEEAGIHSGDS
                     ACVLPPVTLSRTEMKRIRRSTEALARVIGVHGLMNVQYALVSDVLYVLEANPRASRTV
                     PFVAKATGVPLARAAARVMAGESIAELRALGVLPATDASHLDISSPVAVKEAVLPFKR
                     FRTAQGTVVDTVLGPER"
     gene            complement(2793..>3414)
                     /locus_tag="VF362_00385"
     CDS             complement(2793..>3414)
                     /locus_tag="VF362_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779245.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX7589419.1"
                     /translation="VEPPPGTPTRARVVAVDLGIKSMTPHLLAARGVSVTVVPSTITA
                     QEILAMKPDGVFFSNGPGDPGAADHAVQTLRGVLEARVPFFGICFGNQILGRALGFGT
                     YKLRFGHRGINQPVMDRTTGKVEITAHNHGFAVDAPLEGEVQAPYGGFGRVRVSHVNL
                     NDDVVEGLECLDIPAFGVQYHPEAAAGPHDAQYLFDRFVDSMSVGR"
BASE COUNT          519 a         1130 c         1221 g          544 t
ORIGIN      
        1 cctctccggt ccgaggaccg tgtccacgac cgtgccctgc gcggtgcgga agcgcttgaa
       61 gggcagtacc gcctccttca ccgccacggg gctcgagatg tcgaggtgtg acgcgtccgt
      121 cgcgggaagg acgcccaggg cgcgcagttc ggcgatgctc tcccccgcca tgacgcgagc
      181 cgccgcgcga gcgagtggga cgcccgtcgc cttcgcgacg aaggggacgg tccgcgacgc
      241 gcgcgggttg gcctccagga cgtagaggac gtcgctgacg agggcgtact ggacgttcat
      301 cagcccgtgg acgccgatca cgcgagcgag ggcctccgtc gaccgacgga tgcgcttcat
      361 ctcggtgcgc gagagggtga ccggcggcag gacgcacgcg gagtcgcctg aatggatccc
      421 cgcctcctcg atgtgttcca tcacgccgcc gaggaacatt tccaccccgt cgaagagcgc
      481 gtcgacgtcg atctcgatcg cgtcgtccag gaagcggtcc acgaggagcg gggcggccgt
      541 cgcgtggtcg cctacgtcaa cggagcggat gccgtagtcc tcgagttggg cgcggtcgta
      601 gacgatctcc attccgcggc cgccgaggac gtacgacggc cggaccaaca cggggtaccc
      661 gatgcgctcg gcgatgacga tcgcctcgtc gatcccgaag gcggtcccga acgcgggggc
      721 gggaagcccc gcggtcgcaa gaaccttccc gaactcgccg cgatcctccg cgaggttgat
      781 cgcctccggc gaggtcccga ggatcggcac gccggcgtcc gcgaggcgct gcgcgaggct
      841 gaggggggtc tggccgccca gttgcacgat catgcccttg atggggccgg cggccttctc
      901 cgcctcgaag accgccatca cgtcctcgaa cgtcaggggc tcgaagtaca ggcggtcgga
      961 cgtgtcatag tcggtcgaga ccgtctcggg gttgcagttg atcatgatgg tctcgtacgt
     1021 gtccttgagc gccatcgcgg cgtgcacgca tgagtagtcg aactcgatgc cctggccaat
     1081 ccggttgggg ccggacccta ggatgatgac cgcctcgcgc tcgcgggggt gcacctcgtt
     1141 ctccgagtcg taggtcgagt agtggtacgg cgtgctcgcc tcgaactcgg ccgcgcacgt
     1201 gtcgacggtc ttgtagacgg gcgtcacgcc gagcgcgtgc ctcagcgcgt tgacctcgcc
     1261 ctcggtcgtg ccgcggatct cggcgatctg cacgtcggcg agcccgtggg acttcgcgcg
     1321 ccgcagcagt gtcgcgccga gttccggcgc ggccgccacc tccgcggcga cctcgttcat
     1381 caggaggatc tggtcgagga accacgggtc gatcctcgtg gcgtcgaaga cccgatcgag
     1441 gacgccgggg tccgcgatgg ccgcgcgcaa ggcctgttga acctgaagca ggcgccactc
     1501 cgtgggcgtc gcggtggtgg cgatgagatc gtcgagcgcc gagcccgccg ggacctcgcc
     1561 ccgccagtgg aagttgacgc ccttcttgtc gatcgagcgc agggccttgc cgagcgcctc
     1621 cgtgaagctc cgcccgaggc tcatggcctc gccgacggac ttcatcgtgg tcgtgaggac
     1681 cggatccgcc gccgggaact tctcgaaggc gaagcgcgga accttgacga cgacgtagtc
     1741 gagcgtcggc tcgaagctcg cgggggtcga gcccgtgatg tcgttgggga tctcgtcgag
     1801 cgtgtagccg atcgcgaggc gcgcggcgat cttcgcgatg gggaaccccg tcgccttgga
     1861 ggccagggca gaggagcggg gcacgcgggg gttcatctcg atgacgatca cgcggcccgt
     1921 cgagggctgg acggcaaact ggatgttgca ccctccggta tcgacgccca cctcgcgcag
     1981 gacggcgatg ccgatgtccc gtagccgctg gtactcccgg tcggtcagcg tcagggcggg
     2041 cgcgacggtg acggagtccc ccgtgtgtac ccccaccgga tcgatgttct cgatcgagca
     2101 gaccaccacg acgttgtcgt ggcgatcccg catcagctcg agttcgtatt ccttccaccc
     2161 gaggagcgac tcctcgagga gcacctcggt ggtcggcgag tagtggagcc ccgcgcccgc
     2221 gatgcggcgc aggtcatcgg gggtgtacgc gaagcccgag ccaaggccgc ccatggtgaa
     2281 cgaggggcga acgacgacgg gataggtgag cgcgtcggcg gccgccaggc actcgtccat
     2341 cgtgtggcag atgaaggagc gcgcgctctc ggcaccgcag cgcgcgacga catgcttgaa
     2401 ctgctccctg tcctcggcca tatggatgga atcgatcttg gcgccgatga gttcgacgtt
     2461 gtacttctcg aggatccccg cctcaacgag ggcgaccgcg gcgttcagcg cggtctgacc
     2521 ccccagggtc ggcaggaggg cgtcggggcg ctccttggcg atgatcgcct cgatcacctc
     2581 ggtcgtgatc ggctcgatgt acgtggcgtc ggcgaactcg gggtccgtca tgatcgtcgc
     2641 cgggttcgag ttgacgagga tgacgcggat gccctcggcc ttgagcaccc ggcacgcctg
     2701 ggtccccgag tagtcgaact cgcaggcctg gccgatgacg atcgggccgg agccgatcac
     2761 catcacggag cggatatcgt cgcgcctcgg catcagcggc ccacgctcat cgagtcgacg
     2821 aaccggtcga agaggtactg ggcatcgtgg ggtccggcgg cggcctccgg gtggtactgg
     2881 acgccgaacg cggggatgtc gaggcactcg aggccctcga ccacgtcgtc gttgaggttg
     2941 acgtgactca cccgcacgcg cccgaagccg ccgtagggcg cctggacctc gccctcgagc
     3001 ggggcgtcga ccgcgaagcc gtggttgtgg gcggtgatct ccaccttgcc ggtcgtccgg
     3061 tccatcacgg gctggttgat cccgcggtgc ccgaagcgaa gcttgtacgt cccgaatccc
     3121 agggcacggc ccaggatctg gttgccgaag cagatgccga agaacggcac cctcgcctcc
     3181 agcacgcccc gaagcgtctg aacggcgtgg tccgccgcgc cggggtcgcc gggaccgttc
     3241 gagaagaaca cgccgtccgg cttcatggcg aggatctcct gggcggtgat cgtcgagggg
     3301 acgacggtga ccgagacccc gcgcgccgca agcaggtgcg gcgtcatcga cttgatcccg
     3361 aggtcgaccg cgacgacgcg cgccctcgtc ggcgttccgg gcggcggctc gaca
//
LOCUS       DASVKP010000019         2941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1073960, whole genome shotgun sequence.
ACCESSION   DASVKP010000019 DASVKP010000000
VERSION     DASVKP010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073960"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..130
                     /locus_tag="VF362_00390"
     CDS             <1..130
                     /locus_tag="VF362_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005507927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX7589420.1"
                     /translation="DGEVGNKKMYDPRAWGKAAEAGMAARVVEACQQLGSAGKRIK"
     gene            194..1483
                     /locus_tag="VF362_00395"
     CDS             194..1483
                     /locus_tag="VF362_00395"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX7589421.1"
                     /translation="MPGVILVGAQWGDEGKGKATDLLGSRVDYVVKYNGGNNAGHTVV
                     IGGETYALHLLPSGILSPGCIPVIGNGVVVDLGVLFGEIDAIEKRGIDTSRMLISSAA
                     HIITQYSRTMDNVTERFLGSRKIGTTGRGIGPAYADKISRVGIRVGDLFDEALLREKV
                     EGSLEQKNHLLVKVYNRRAISADEVVDELLSYVPRLAPMVADTSLALNQALDRGETVL
                     FEGGQATMLDVDHGTYPFVTSSNATAGGACTGTGVGPTRINRVVGVLKAYTTRVGEGP
                     MPTELLDASGERLRAVGHEYGTTTGRPRRCGWYDAVVARYANRVNGLTDFVLTKLDVL
                     TGLERIPVCVAYDVGGVRHEELPLDQSAFGAAVPIYEEFEGWTEDITGARTFEDLPAA
                     AQRYVLALEKVSGCRMSVIGVGPGREEIVVRHDLLGE"
     gene            1551..2819
                     /gene="purD"
                     /locus_tag="VF362_00400"
     CDS             1551..2819
                     /gene="purD"
                     /locus_tag="VF362_00400"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004005520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX7589422.1"
                     /translation="MKVLVIGSGAREHAIARSLRSDPGVTQIHAAPGNPGIGHVAELH
                     EVDPLDPTAVAALATHLGVDLVVLGPEAPLVAGVADAVRGAGIACFGPSAAAAMLEGS
                     KAFAKHVMEAAGVPTARARLCEGPETLGAALDEFGAPFVVKDDGLAAGKGVVVTRDRG
                     EAFAHGNAVLAGGGKVVVEEFLDGPEVSLFCISDGTTVVALEPAQDFKRVNDGDEGPN
                     TGGMGAYSPLPWAPPTLVEEVLETVARPTIAEMARRGTPFQGVLYCGLALTSAGLRVI
                     EFNARFGDPETQSVLARLLTPLSTLLHAAATGTLADLAPLEWSPESAVTVVIAASGYP
                     AAPRTGDPISGTESAEGEAGVHVLHAGTALGDGGELVSAGGRVLSIVGVGDGLAAARE
                     RAYAGVDRIGLEGSHHRTDIALAAANMEDR"
BASE COUNT          452 a         1049 c         1017 g          423 t
ORIGIN      
        1 cgacggcgag gtcggcaaca agaagatgta cgacccgcgc gcgtggggca aggccgccga
       61 ggcgggtatg gccgctcgcg tcgtcgaggc gtgtcagcag ctcggaagtg caggcaagag
      121 aatcaagtaa cgagaccgcg agagtccccg cggtccacgg ccgcggggac ttttcgcgtg
      181 taaggagcgc atgatgcccg gagtgattct ggtcggagcc caatggggcg acgagggtaa
      241 gggcaaggcg accgatcttc tcggttcccg cgtcgactac gtcgtcaagt acaacggcgg
      301 gaacaacgcc gggcacacgg tggtcatcgg gggcgagacc tacgcgctcc acctcctgcc
      361 ctccggcatc ctctctcccg gatgcattcc cgtcatcggc aacggcgtgg tggttgacct
      421 cggcgtcctc ttcggcgaga tcgacgcgat cgagaagcgc ggcatcgaca cgtcccgcat
      481 gctgatctcg tccgcggcgc acatcatcac ccagtactcg cggaccatgg acaacgtcac
      541 cgagcggttc ctcggctcgc gcaagatcgg gacgaccggg cgcggcatcg gtccggcgta
      601 cgccgacaag atcagccggg ttggcattcg cgtgggagac ctcttcgatg aggcgctcct
      661 gcgggagaag gtggagggtt cgctcgagca gaagaaccat ttgctcgtga aggtctacaa
      721 ccgcagggcg atcagcgcgg acgaggtcgt tgacgaactg ctctcctatg tccctcgcct
      781 cgcaccgatg gtcgccgaca cctctctggc cctgaaccag gcgctggatc gcggcgagac
      841 cgtcctcttc gagggcggcc aggcgaccat gctcgacgtc gaccacggaa cctacccctt
      901 cgtgacatcc tccaacgcga cggcgggcgg ggcctgcacg ggcacgggcg tcggcccgac
      961 gcgcatcaac cgggtggtcg gcgtgctcaa ggcctacacg acgcgcgtgg gcgagggccc
     1021 gatgcccacc gaacttctgg acgcgtccgg cgagcgcctc cgcgccgtgg gccacgaata
     1081 cgggacgacc acgggccggc cgcgccgctg cggctggtac gacgccgtcg tcgcccgcta
     1141 cgccaaccgc gtcaacggcc tgacggactt cgtcctcacc aagctcgacg ttctgacggg
     1201 gctcgagcgg attccggtct gcgtcgcgta cgacgtcggc ggcgtgcggc acgaggaact
     1261 cccgctcgac cagtccgcct tcggggcggc cgtgcccatc tacgaggaat tcgagggctg
     1321 gaccgaggac atcaccgggg cgcggacctt tgaggatctg cccgcggccg cgcagcgata
     1381 cgtgctggcg ctcgagaagg tttcgggctg caggatgagc gtcatcggag tcgggccggg
     1441 gcgcgaggag atcgtcgttc ggcacgacct gctcggcgag tagcgaacca agaccatcgc
     1501 gttggggtca agccaccgcc gggaaggtgt gccccggtag agttgccacc atgaaggtcc
     1561 tcgtcatcgg ctcgggcgcg cgcgagcacg ccatcgcccg ctcgcttcgc tcggatcccg
     1621 gggtcaccca gatccacgcg gcccccggca accccgggat cggccacgtc gccgaactcc
     1681 acgaggtgga tcccctcgac cccacggccg tcgccgcact cgcgacgcac ctcggagtcg
     1741 acctcgtcgt cctgggaccc gaggctccgc tcgtcgcggg ggtagccgac gccgttcgag
     1801 gcgcgggcat cgcgtgcttc ggtccgagcg cggcagccgc gatgctcgag ggatccaagg
     1861 ccttcgcgaa gcacgtcatg gaagcggccg gtgtccccac ggcccgcgcc cggctctgcg
     1921 agggacccga aaccctcggc gcggcgctcg atgagtttgg ggcccccttc gtggtcaagg
     1981 acgacgggct ggccgcgggc aagggcgtcg tcgtgacgcg cgaccgcggc gaggccttcg
     2041 cccacgggaa cgccgtcctc gccgggggcg gcaaggtcgt cgtcgaggag ttccttgacg
     2101 gtcccgaggt gtccctcttc tgcatctccg acggcacgac cgtggtggct ctcgagccgg
     2161 cccaggattt caaacgcgtg aacgacggag acgagggtcc gaacacgggc gggatgggcg
     2221 cctacagccc gctgccctgg gcccccccga cgctcgtcga ggaggtgctc gagacggtcg
     2281 cgcgccccac gatcgccgag atggcccgac gcggcacgcc gttccagggc gtcctctact
     2341 gcgggctggc gctcacctcc gccggactcc gcgtgatcga gttcaacgcg cgcttcgggg
     2401 acccggagac ccagtccgtc ctggcacgtc tcctgacgcc gctgtcgacc ctccttcacg
     2461 cggcggccac gggcacgctc gcggacctcg cgcctctcga gtggagcccc gagtccgcgg
     2521 tcacggtcgt catcgcggcg agcgggtatc ccgccgcgcc ccgcacgggc gaccccatct
     2581 ccggtacgga gtcggcggag ggcgaggccg gcgtccacgt gctgcacgcc ggcaccgcgc
     2641 tcggggacgg cggggaactc gtctccgcag gcgggcgcgt cctgtcgatc gtcggcgtgg
     2701 gcgatgggct cgccgccgcg cgcgaacgcg cctacgcggg ggtggaccgc atcgggctcg
     2761 aaggctccca ccaccgcacc gacatcgccc tagccgccgc gaacatggag gaccgatgaa
     2821 cgctcccacc ccgccggtcg cgccacccat tccgggctgg cgccacatct actccggcaa
     2881 ggtccgcgac ctctacgagc cagccgagcc tcaccccaac ggcgacgtcc tcctggtcgt
     2941 c
//
LOCUS       DASVKP010000020         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1321444, whole genome shotgun sequence.
ACCESSION   DASVKP010000020 DASVKP010000000
VERSION     DASVKP010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321444"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..819
                     /locus_tag="VF362_00405"
     CDS             151..819
                     /locus_tag="VF362_00405"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX7589423.1"
                     /translation="MAVSLAHERSLWDAGATVVAGIDEVGRGALAGPVSVGVVALCRC
                     DAWPDGLADSKQLTPAQREAMAVALATFGVARAVGDASNAEIDALGIVAALRVAALRA
                     LEAIAASGLIVDAILLDGKHDYLTAKAPDLFASSSAGESDVEIPPVTMVVGGDALCAS
                     VAAGSVLAKVARDAQMRAAHDGHPEYGWVGNKGYGAPEHLDAIRRLGPTPLHRVSWKL
                     PARS"
     gene            883..1215
                     /locus_tag="VF362_00410"
     CDS             883..1215
                     /locus_tag="VF362_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012244516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HEX7589424.1"
                     /translation="MSHDDLENYETEAELALYREYRDVVGLFTYVVETERRFYLANKV
                     DLEVKHAGHDVYFDLTLTDAWVWDVYRSARLVKNVRVVTFRDVNVEELAHDNDIPDLR
                     ALRGPRRR"
     gene            1332..1736
                     /locus_tag="VF362_00415"
     CDS             1332..1736
                     /locus_tag="VF362_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX7589425.1"
                     /translation="MAAKDELGAHGEEIATRELLGLGWSVLARNWRPTPGRGLPRGEI
                     DIVAQDGRELVIVEVKTRSSRTFGGAVEAVTRAKLARLRRLAAAWLASQDRRIDGARI
                     DGARFDGVRIDVICIDIGRDGRPRVEILRGVE"
     gene            1733..>2381
                     /locus_tag="VF362_00420"
     CDS             1733..>2381
                     /locus_tag="VF362_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEX7589426.1"
                     /translation="MIGRTRSIALAGLDGYVIDVEAHMAASLPAFTIVGLPDASLNEA
                     RDRVRAAVTSSNLPWPNRRITVNLSPASLPKTGSATDLSIAVAVLAAAGLIDRAAARG
                     VVHLGELGLDGRVRPVRGVLPAVAEAVRAGATDVVVPTGNLDEALLVPGAVPVGVATL
                     AELAARYGNEAARPASTEVVLPATRPRAEAEGLDLADVLGQEEPRWALEVAAAGGH"
BASE COUNT          310 a          860 c          880 g          331 t
ORIGIN      
        1 gccgctcgat cactggaggg gactcggcaa tcccttcccc gcggtcgccg cgccgacgtc
       61 cggggccgcg ccgacgtccg gggccgcgcc gacgtccgga gccgcgtcga cgcccggggc
      121 cgcgccgacg tccggagcca ccggctagtc atggccgtca gcctcgcgca tgagcgatcc
      181 ctgtgggacg ccggcgcgac cgtcgtcgcg ggcatcgacg aggtcggccg cggcgccctc
      241 gccggccccg tctccgtcgg cgtcgtcgcc ctctgccgat gcgacgcgtg gcccgacggc
      301 ctcgcggact ccaaacaact gaccccggcc cagcgcgagg cgatggcggt tgcgctcgcg
      361 accttcggcg tcgcgagggc ggtgggcgac gcgtccaacg ccgagatcga cgccctgggc
      421 atcgtcgcgg cgcttcgcgt cgcggccctt cgcgcgctcg aggcgatcgc ggcgagcggg
      481 ttgatcgttg acgcgatcct cctcgacggc aagcacgact acctcaccgc caaggcgccc
      541 gatctcttcg catcgtcgtc ggctggcgaa tccgacgtcg agattccgcc cgtgacgatg
      601 gtcgtcgggg gagacgcgct gtgcgcgtcg gtcgccgcag ggagcgtcct cgcgaaggtg
      661 gcgcgcgacg cccagatgcg cgcggcccac gacgggcatc cggagtacgg gtgggtgggc
      721 aacaagggct acggcgcacc cgaacacctc gacgcgatcc ggcgactggg accgacgccc
      781 ctccaccgcg tctcgtggaa gctccccgcg cggtcgtagg gggtgcggtc gtaggccgcg
      841 cgccggttcg cgagcgcgcg ccgcgcgcgg catgatgggg cgatgagcca cgacgacctg
      901 gagaactacg agaccgaggc cgagctggcc ctgtatcgcg agtaccgcga cgtggtcggg
      961 ctcttcacct acgtggtcga gaccgagcgg cgcttctacc tggcgaacaa ggtggaccta
     1021 gaggtcaagc acgccggcca cgacgtctac ttcgacctca ccctgaccga cgcgtgggtg
     1081 tgggacgtgt atcgctccgc gcggctcgtg aagaacgtgc gggtggtgac gttccgcgac
     1141 gtcaacgtcg aggaactcgc ccacgacaac gacatcccgg accttcgggc gctgcggggc
     1201 ccgcggaggc ggtaggcctg cgggcggccc gatgtcccca ggcgcctcgc ccgctcgctg
     1261 accccccgcc gagtcccgcc ccgcccgacg ggtcccggcc gcgcgccacc gttgtcgcgg
     1321 gaggtgtcgc gatggccgcg aaggatgagc tcggcgcgca cggcgaggag atcgcgacgc
     1381 gggaactcct gggcctgggg tggtcggtgc tcgcgcgcaa ttggcgcccg acccccggaa
     1441 ggggcctgcc gcggggagag atcgacatcg tggcgcagga cggccgcgaa ctcgtcatcg
     1501 tcgaggtcaa gacgcggagc agccgaacct ttggcggcgc ggttgaggcc gtgacgcgcg
     1561 ccaagctcgc ccgcctccgt cgcctcgcgg cggcctggct cgcgagccag gatcggcgca
     1621 tcgacggggc gcgcatcgac ggggcgcgct tcgacggggt gcgcatcgac gtgatctgca
     1681 tcgacatcgg gcgcgacggg cgaccccgcg tcgagatcct gcggggcgtc gagtgattgg
     1741 tcgcacccgc tcgattgccc tcgccgggct cgatgggtac gtcattgacg tcgaggccca
     1801 tatggcggcg tcacttccgg cgttcacgat cgtcggcctc cccgacgctt ccctcaacga
     1861 ggcgagggat cgggttcggg ccgccgtcac gtcgagcaat ctgccctggc ccaaccgacg
     1921 catcacggtc aacctctcgc cggcgtcgct ccccaagacc gggtccgcga cggatctctc
     1981 catcgcggtg gcggtgctgg ctgccgccgg actcatcgat cgggccgccg cgcggggagt
     2041 ggttcacctc ggcgaactgg gcctcgatgg tcgcgtccgc cccgtgcgcg gggtcctccc
     2101 cgcggtcgcc gaggccgttc gggcgggggc cacggacgtc gtcgttccga cgggcaacct
     2161 cgacgaggcc ctgctcgttc ccggagccgt accggtcggg gtggccaccc tggcggaact
     2221 cgcggcgcgc tacggcaatg aagcggcgcg ccccgcctcg accgaggtgg tgctgccggc
     2281 aactcggcct cgcgcggagg cggagggcct cgaccttgcc gacgttctgg gtcaggagga
     2341 gccgcgctgg gcgctcgagg tggccgcagc gggcggtcac c
//
LOCUS       DASVKP010000021         4596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_828328, whole genome shotgun sequence.
ACCESSION   DASVKP010000021 DASVKP010000000
VERSION     DASVKP010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828328"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VF362_00425"
     CDS             complement(<1..408)
                     /locus_tag="VF362_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX7589427.1"
                     /translation="MFASLSDRLAETFRNLRGKGRLSEADVDATVREIRRALLDADVA
                     LPAVKAFTAAIRERALGAEVASALNPAQQVVSIVHDELVEILGGASRPLARAKTGPTV
                     IMLAGLQGAGKTTLAGKLGRHLKDLGETPVLVAA"
     gene            complement(479..2227)
                     /locus_tag="VF362_00430"
     CDS             complement(479..2227)
                     /locus_tag="VF362_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7589428.1"
                     /translation="MRDLKRDRLALCGRLFDAKVPGAERREAVGDLVLARLAEHWEEA
                     SGGSTTGVALAAVGSIARRDAGPASDLDLLLIHDGSTMKKEELAALAQRLWYPIWDAG
                     LELDHSMRSLTECRQVATSDLAAAAGLLDLRFVGGDPALVQQARASILADWRSAARHR
                     LADLLASSRARAERFGEVAYLIEPNLKESRGGLRDLVSLSALAATWLADRPHGAVDRA
                     GDHLLDVRDALHLEAGRSQVVLGRHVARKVALRLGFGHPDDLLASLAEAGRVIAFALD
                     ATERNARRSLEKSGHGSRAFLARRRNVAPRYVSMGEGLIDVDGDLALAASAVPQDDAL
                     LTLRAGAAAATTGLRMTPGLLEALARCPNLPTPWPAEARAYLTEMLGASANLVAVWEA
                     LDLAGIVVRWFPEWEAVRNRPQRSPVHRFTVDRHSVEAVVRAGRLKRNVQDPALLLLA
                     CLFHDLGKRAGARDHSITGADLVPGMAARMGLAPTFAGDLEILVRHHLLLADLATRQD
                     PEDAATARALLEPLGYRRDLLNTLRALTEADATAAGPKAWTPWREKLCDALTRTAHRE
                     IDARGAAANAKRGGPA"
     gene            complement(2296..2634)
                     /locus_tag="VF362_00435"
     CDS             complement(2296..2634)
                     /locus_tag="VF362_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7589429.1"
                     /translation="MKLVTAIIQPHRLEEVRTALEGVGVKGVTVSEASGYGRQKGHTE
                     VYRGAEYTVDLLPKIRIEVVADDADVKAVTDAIVGAAQTGEIGDGKIWIVKVEDIVRV
                     RTGERGPKAI"
     gene            complement(2637..3899)
                     /locus_tag="VF362_00440"
     CDS             complement(2637..3899)
                     /locus_tag="VF362_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX7589430.1"
                     /translation="MGFTLDSGNTAWMITATALVLLMTPALAFFYGGMVRTKSVLNMM
                     MMSFVSLGTVAVIWVIYAYGLAFGPGSVIGSPRGLVGLNYVSDSRSLGAAVGVPFLVM
                     VAFQATFAIITVALISGAIADRMKFGAWVIFTVVWITLVYTPIAHAVWGGGTLITSLT
                     QSTPIDFAGGTVVHINAGAAGLVLAILLGKRRGFGKEPMRPHNLPFVMLGAALLWFGW
                     FGFNAGSEFAADATAGRAFLNTTIAPAFALLAWILVEKIRDGKPTSLGAASGIVAGLV
                     AITPAAASVDTWGALAIGFIAGVVCSLAVGLKYKFGYDDSLDVVGVHLVGGIAGTLLI
                     GLFANLKEGTTWAPKGAFSGLFYGGGWTQLGTQAIAAGIAVTYSLVATLIIGLALKYT
                     VGLRVKESVEVGGIDLAEHGETAYEEAN"
     gene            complement(4052..>4596)
                     /locus_tag="VF362_00445"
     CDS             complement(4052..>4596)
                     /locus_tag="VF362_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX7589431.1"
                     /translation="ARLLVADGYGVVLAGADTFRAAAADQLETWGARAGVTVVRSDRP
                     GGDPGAVVFEAGERARESRADVVLVDTAGRLHNKRDLMDELGKILRVVSRVRPPAEVL
                     LVLDATTGQNGLTQARVFTEVAGVTGVILAKLDGSARGGVVIAIERELGVPVKFVGVG
                     ERADDLAPFDPEAFVDGLLA"
BASE COUNT          724 a         1601 c         1641 g          630 t
ORIGIN      
        1 cgcggcgacc aggaccggcg tctcccccag gtccttgagg tggcgcccga gcttgcccgc
       61 gagcgtcgtc ttgcccgccc cctggagccc ggcgagcatg atgaccgtcg ggccggtctt
      121 ggcgcgggcg agtggccgcg acgcaccccc gaggatctcc acgagctcgt cgtggacgat
      181 cgaaacgacc tgctgcgcgg ggttgagggc gctcgcgacc tccgccccga gggcccgctc
      241 gcggatcgcg gcggtgaagg ccttgaccgc gggaagggcg acgtccgcgt cgagcaacgc
      301 gcggcgaatc tcgcgaacgg tcgcgtcgac gtcggcctcg ctcaggcggc ccttgccgcg
      361 caggttgcgg aacgtctcgg cgaggcgatc cgagagcgac gcgaacacgc ggctccttcc
      421 tagggaggtg cggccggcgc gcgccggtcg aaggctccag attacccggg cgcgacgcct
      481 acgcggggcc gccccgcttc gcgttcgccg cggcgccgcg ggcgtcgatc tcgcggtggg
      541 ccgtccgggt gagcgcgtcg cacagcttct ccctccacgg cgtccacgcc ttgggccccg
      601 ccgcggtggc gtccgcctcg gtcagcgcgc ggagcgtgtt gagcaggtcc cgccgatacc
      661 cgagcggctc gaggagggcg cgggcggtcg ccgcatcctc gggatcctgc cgggtcgcga
      721 gatcggcgag caggaggtga tgccgcacga ggatctcgag gtcgcccgcg aaggtgggcg
      781 ccaggcccat gcgcgcggcc atcccgggca ccaggtccgc accggtgatg gagtggtccc
      841 tcgcccccgc gcgcttgccg aggtcgtgga acaggcacgc caagagcagg agggcgggat
      901 cctggacgtt gcgcttgagc ctgcccgcgc gcacgacggc ctcgaccgag tggcggtcca
      961 cggtgaatcg gtggacgggc gagcgctgag ggcgattgcg caccgcctcc cactcgggga
     1021 accagcgcac cacgatcccg gcgaggtcga gcgcctccca cacggcgacg aggttcgcac
     1081 tcgcaccgag catctccgtc aggtacgcgc gggcctcggc cggccacggc gtcgggaggt
     1141 tggggcagcg cgccagggcc tcaagcagcc ccggcgtcat gcgcagcccc gtcgtcgcgg
     1201 ccgcggcacc cgcccgaagc gtgaggagcg cgtcgtcctg gggaaccgcg ctcgccgcca
     1261 gcgcgaggtc gccgtcgacg tcgatcaggc cctctcccat cgagacgtac cgtggcgcga
     1321 cgttgcgccg ccgggccagg aaggcgcggg acccgtgccc ggacttctcg agagaacgac
     1381 gagcgttgcg ctcggtggcg tcgagcgcga aggcgatcac gcggcccgct tccgcgagcg
     1441 aggcgagcag gtcgtcgggg tgcccgaagc ccaggcggag ggcgaccttg cgggccacgt
     1501 ggcggccaag gaccacctgc gagcgccccg cctcgaggtg cagggcatcg cgcacgtcga
     1561 ggaggtggtc tcccgcgcga tcgacggcgc cgtgggggcg gtcggcgagc caggtcgcgg
     1621 cgagggcgga gaggctcacc aggtcccgca gccccccgcg agactccttg aggttgggct
     1681 cgatgaggta ggcgacctcg ccgaagcgct ccgcgcgggc gcgcgaagag gccaggaggt
     1741 ccgcaaggcg gtggcgggcg gcgctacgcc agtcggcgag gatcgaggcg cgcgcctgct
     1801 ggacgagcgc cggatcgccg ccaacgaatc gaaggtcgag gaggcccgcc gcggccgcga
     1861 ggtcgctcgt cgcgacctgc cggcactcgg tgagcgagcg catggagtgg tcgagctcga
     1921 gccccgcgtc ccagatggga taccagaggc gctgcgcgag cgcggccagc tcctccttct
     1981 tcatggtgga gccgtcgtga atgagaagga ggtcaaggtc gctcgcgggg ccagcatcgc
     2041 gacgggcgat gctcccgacg gccgcgagcg cgacccccgt ggtggacccg cccgaggcct
     2101 cctcccagtg ttcggcgagg cgcgcaagga cgaggtcccc cacggcctcg cgccgctcgg
     2161 cgcccgggac cttcgcgtcg aagaggcggc cacagagcgc gagcctgtca cgcttgaggt
     2221 cccgcacccc ggaagggtgc gggacctcgg cgcgagagcc cggctggccg gaaccagagt
     2281 ggctcggcat ggtacctaga ttgccttggg gccgcgctcg cccgtacgga ctcgcacgat
     2341 gtcctctacc ttcacgatcc agatcttgcc gtcgccgatc tcgcccgtct gggccgcgcc
     2401 gacgattgcg tcggtgacgg ccttgacgtc ggcgtcgtcc gcgacgacct cgatgcggat
     2461 cttggggaga aggtcgacgg tgtattccgc accgcgatag acctccgtgt ggcccttctg
     2521 gcgaccgtag ccggacgcct ccgagaccgt cacacccttg actccgacgc cctcaagggc
     2581 ggtccggact tcctcaagac ggtgcggctg gatgatggct gttacgagct tcatagttag
     2641 ttcgcctcct cgtaagcggt ctcgccgtgc tcggcgaggt cgatgccgcc gacctcgacg
     2701 gactccttca cgcgcagacc caccgtgtac ttcagcgcga gcccaatgat cagcgtagcg
     2761 acaagcgagt aggtcacggc aatcccagcc gcgatcgcct gggtacccaa ctgggtccaa
     2821 cctccgccat agaagaggcc actgaatgcg cccttgggtg cccaggtcgt gccctccttg
     2881 aggttcgcga agagcccaat gagcagggtt cccgcgatgc cgccgacgag gtgaacgccc
     2941 acgacgtcga gcgagtcgtc gtagccgaac ttgtacttga gcccgactgc cagcgaacac
     3001 acgacgccgg cgatgaagcc aatggcgagc gcgccccacg tgtcgaccga ggccgccgca
     3061 ggggtgatgg caacaaggcc agcgacgatg ccggaggccg caccgagcga ggtgggcttc
     3121 ccgtcgcgga tcttctccac aaggatccag gcgagtagcg cgaacgccgg cgcgatggtc
     3181 gtgttgagga acgcccggcc tgcggtcgcg tcggcggcga actcggagcc ggcgttgaag
     3241 ccgaaccacc cgaaccacag aagcgctgcc ccgagcatga cgaacggcag gttgtgcgga
     3301 cgcatcggct ccttgccgaa gcccctgcgc ttgcccaaga ggatggcaag gacgaggccc
     3361 gcggcgccgg cgttgatgtg aaccaccgtg ccacccgcga agtcgatggg agtcgactgc
     3421 gtgagcgaag tgatgagcgt gcctccaccc cacaccgcgt gagcgatggg cgtgtagacg
     3481 agcgtgatcc acaccacggt gaagatcacc cacgcgccga acttcatgcg gtcggcgatc
     3541 gcgccgctaa tgagcgcgac ggtgatgatc gcgaaggtgg cctggaaggc gaccatcacg
     3601 aggaacggca cgccaactgc cgcgcccaga ctgcgtgagt cggaaacgta gttcagtccg
     3661 accaggcccc tcgggcttcc gatgacgctg cccggtccga aggcaagtcc atacgcgtag
     3721 atgacccaga tgaccgcgac cgtgcccagg ctgacgaaag acatcatcat catgttgagc
     3781 acggacttgg tgcgaaccat gccgccatag aagaacgcca aggctggcgt catgagcagc
     3841 acgagagcgg tcgcggtgat catccacgcg gtgttgccac tatccagcgt gaatcccatt
     3901 gttcgtcttc tcctctcgcc agccggtcgg ctgacggact aagagtcccg cgttgaggtt
     3961 tcgccccggc cacggcggtg tttcgcggcc gttacacgtg ggccccgcgt gtaaaggttg
     4021 cgttgccgca agggggcgca gggccacgga actaggcgag caacccgtcg acgaacgcct
     4081 cgggatcgaa cggggcgagg tcatcggcgc gctcgccgac gccaacgaac ttcacgggga
     4141 cgcccaactc gcgctcgatc gcgatcacga cgccgccccg cgctgagccg tcgagcttcg
     4201 cgaggatgac ccccgtgacc cccgcgacct cggtgaagac gcgcgcctgc gtgagcccgt
     4261 tctgacccgt cgtcgcgtcg agcacgagga ggacctccgc gggggggcgg acgcgcgaga
     4321 caacccggag gatcttcccg agctcgtcca tcaggtctcg cttgttgtgc agccggccgg
     4381 cggtgtcgac gagcacgacg tccgcccggg actccctcgc gcgctccccc gcctcgaaca
     4441 ccacggctcc gggatcgccg cccggtcggt cggatctcac gacggtgacg cccgccctcg
     4501 cgccccaggt ctcgagttga tccgcggccg cggcgcggaa ggtgtcggcc cccgccagga
     4561 cgacgccgta tccgtccgcg acgagcaggc gggcga
//
LOCUS       DASVKP010000022         2922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1321933, whole genome shotgun sequence.
ACCESSION   DASVKP010000022 DASVKP010000000
VERSION     DASVKP010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321933"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1330
                     /locus_tag="VF362_00450"
     CDS             95..1330
                     /locus_tag="VF362_00450"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEX7589432.1"
                     /translation="MTDEPDLSKYRPDTRAIRGGIERSQHHEMSEGLFMTQGYVYDRA
                     ADAEAAFAGELDVYMYARYGNPTVTAFQDRLRAIEGADACFATATGMSAIFTALASIL
                     TSGDRLVASRALFGSSIQVFNLYFANWGIEVEFVDGHVLDQWEAALARPAKAVFFETP
                     TNPMQDIVDVRRVAELAHAAGAMVIVDNVFATPIGQRPLEMGADAVIYSATKHIDGQG
                     RVMGGAILGSDEYVNGPVKMMVRQMGPTLSPFNAWVLLKGLETMPVRVRAQAASALAI
                     AEFLEGRAGVARVLYPFLNSHPQVELARSQMSIGGTVVTFDLAAKGGGNRGGAAQGSD
                     TKAAAFRVMDALHLFELSNNLGDAKSIVTHPATTFHRSLGADGRAAIGESDGTIRLSV
                     GLEDVEDLKEDLDRALGAL"
     gene            1379..1903
                     /locus_tag="VF362_00455"
     CDS             1379..1903
                     /locus_tag="VF362_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589433.1"
                     /translation="MFKRKQPPPSSPLRLDPAIISLRRSALSGSIDDLGQRRDPMPPV
                     WLAVMEWVVGDSAVTLVSVNDGSTSLYFSHGGGFIGAGTHEPVAIATGQFLGECYLAR
                     AQFAPAMGFDFPPVGVVRFYCRTDEETLTASAPEDEVRERDHPLFRPWAAAQAVISQI
                     RQHSPSPGAGAPPA"
     gene            complement(1900..>2922)
                     /locus_tag="VF362_00460"
     CDS             complement(1900..>2922)
                     /locus_tag="VF362_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX7589434.1"
                     /translation="GTVTEGRMVATDVVAPGFDDEARRYLIEAIASAEKDSEHPVGRA
                     VAALVPTRRGVKDFRITAGQGVTATVEGSRDATGKGKKCADREPSVVRVGSRRMFDSI
                     APELEEAAAAAEALGNTAVFAGRTPVPTSTALHPNTLPAASALISVRTPASAEAVVVV
                     RDAMKPSSRGAVAALKKLGLEVVLLTGDNERSAKAVAAEVGIDRVYAEVLPGEKADVV
                     TKLQAEGKRVAMVGDGINDAPALAQADLGIAIGTGSDVAREASDLTIVSGDLMAAVDA
                     IRLARRTFSTIRGNLFWAFAYNVAMIPLAAFGVLNPMIAAGAMGSSSLFVVGNSMRLR
                     GFKAAR"
BASE COUNT          428 a         1066 c          997 g          431 t
ORIGIN      
        1 ggcttccctg gcaccagggc tagttcgtag cgtcgggcgg agcgacttcc gccgcttctc
       61 gaggcttgcc gcgccgagtc cctagactgg ccgcatgacc gacgagcccg atctcagcaa
      121 gtaccgcccc gacacccggg cgatccgcgg cgggatcgag cgctcgcagc accacgagat
      181 gtccgagggt ctcttcatga cccagggcta cgtctacgac cgcgccgccg acgccgaggc
      241 agccttcgcg ggcgagctcg acgtgtacat gtacgcgcgg tacggaaacc ccacggtgac
      301 ggcgttccag gatcggctgc gcgcgatcga gggcgccgac gcctgcttcg cgaccgcgac
      361 cggcatgtcc gcgatcttca ccgccctcgc gtcgatcctc acctcgggcg accggctcgt
      421 cgcgtcgcgc gcgctgttcg gatcgtccat ccaggtcttc aacctgtact tcgccaactg
      481 gggcatcgag gtggagttcg tcgacggcca cgtgctcgac cagtgggagg cggcgctcgc
      541 ccgtcccgcg aaggcggtct tcttcgagac gcccaccaac ccgatgcagg acatcgtcga
      601 cgtccgccgc gtcgccgagc tcgcccatgc ggccggcgcc atggtcatcg tcgacaacgt
      661 cttcgcgacc cccatcggcc agcggccctt ggagatgggc gccgacgcgg tcatctactc
      721 cgcgacgaag cacatcgacg gccagggtcg cgtgatgggc ggggccatcc ttgggagcga
      781 cgagtacgtc aacggcccgg tcaagatgat ggtccggcag atgggcccca ccctctcgcc
      841 cttcaacgcc tgggtgctgc tcaagggcct ggagacgatg cccgtgaggg tgcgggcgca
      901 ggcggcgagc gcgctcgcga tcgccgagtt cctcgagggc cgcgcggggg tcgcgcgcgt
      961 cctctacccg ttcctcaact cgcacccgca ggtggaactc gcgcggtccc agatgtcgat
     1021 cggcgggacg gtcgtcacgt tcgacctcgc cgccaagggg ggcggcaaca ggggcggcgc
     1081 cgcgcagggc tccgacacca aggccgcggc cttccgggtc atggacgcgc tacacctctt
     1141 cgagttgtcc aacaacctgg gcgacgccaa gtccatcgtc acccacccgg cgacgacctt
     1201 ccaccggagc ctcggcgctg acggccgcgc ggccatcggc gagtccgacg ggaccatccg
     1261 cctcagcgtc ggcctcgagg acgtcgagga ccttaaggaa gacctcgacc gggcgctcgg
     1321 ggcgctgtag cgacaccccg cgccaccgtc ccgcgcgctc cgcccgtagg ctggcggcat
     1381 gttcaagcga aagcagccac ccccgtcgag tcccctacgc ctcgacccgg cgatcatcag
     1441 tctgcgccgg agcgcgctgt cggggtccat cgatgacctg ggccagcgcc gcgacccgat
     1501 gccgcccgtg tggctcgcgg tcatggagtg ggtggtcggc gacagcgccg tcacgctcgt
     1561 ctccgtgaat gacgggtcga cgtcgctcta cttcagccat ggcggcggct tcatcggcgc
     1621 cggaacccac gagcccgtcg cgatcgcgac cgggcaattc ctgggcgagt gctacctggc
     1681 gcgggcccag ttcgctcccg cgatgggctt cgacttcccc ccggtcggag tggttcgctt
     1741 ctactgtcga accgacgagg agaccctcac ggcgagcgct cccgaggacg aggtccgtga
     1801 gcgcgaccac ccgttgttcc gaccctgggc cgccgcgcag gccgtcatca gtcaaatccg
     1861 ccagcacagc ccctcgccgg gggcgggagc ccctcccgcc tagcgtgcgg ccttgaaccc
     1921 gcggaggcgc atcgagttgc cgacgacgaa gagggatgaa ctccccatcg cgccggcggc
     1981 gatcatcggg ttgagaaccc cgaacgcggc gagagggatc atcgcgacgt tgtaggcgaa
     2041 cgcccagaag aggttgccgc ggatggtcga gaacgtgcgg cgggcgaggc ggatcgcgtc
     2101 cacggcggcc atgaggtcgc ccgagacgat ggtcaggtcg gacgcctccc gcgccacatc
     2161 gctccccgtg ccaatcgcga tgccgaggtc cgcctgcgcg agcgccgggg cgtcgttgat
     2221 tccgtccccg accatcgcga cgcgcttgcc ctctgcttgg agtttggtca cgacgtcggc
     2281 cttctccccg gggagcacct ccgcgtagac ccgatcgatg ccaacctcgg ccgcgacggc
     2341 cttggccgag cgctcgttgt ctccggtgag gagcacgacc tcgaggccga gcttcttcag
     2401 cgcggccacc gcgccgcgcg acgagggctt catcgcgtcg cggacaacca cgacggcctc
     2461 ggccgacgcg ggagtccgga cggagataag cgcgctagcg gcgggcaagg tgtttggatg
     2521 aagcgccgtg ctggtcggaa ccggcgtgcg gcccgcgaag acggcggtgt tgccgagcgc
     2581 ctccgcggcc gcggcggcct cctcaagctc cggggcgatg gagtcgaaca tccggcggga
     2641 acccacgcga accacggagg gctcccggtc ggcgcacttc ttgcccttcc ccgtcgcgtc
     2701 tcgcgaaccc tcgaccgtcg ccgtcacgcc ctggcccgcg gtgatacgga agtccttgac
     2761 gccgcggcgg gtcgggacga gcgccgcgac cgcgcgtccg accgggtgct cggagtcctt
     2821 ctccgccgag gcgatcgcct cgatcaggta gcggcgcgcc tcgtcgtcga agccgggggc
     2881 gacgacgtcc gtcgcaacca tccggccctc ggtgacggtg cc
//
LOCUS       DASVKP010000023         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_829024, whole genome shotgun sequence.
ACCESSION   DASVKP010000023 DASVKP010000000
VERSION     DASVKP010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829024"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..>1649
                     /locus_tag="VF362_00465"
     CDS             393..>1649
                     /locus_tag="VF362_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX7589435.1"
                     /translation="MSRSIYIASAEGDCGKSTVALGVVDHLSRTIDKVGIFRPVARVN
                     DGSDYVLQLLLGHIGVDLSYDECVGVTYDDVHRDPDAALSTIVSRYHEVERKCDAVVI
                     VGTDYTDLAAAAEFEYNARIAANLGSPVALVVRGAGRSPAEVQQAIESARLELESSHA
                     RLAAVFVNKANPDDVEEIRAALPAGLGMGVLPEDPILVAPLLSELMTAVGGELYSGDA
                     KLLGREVLGVIIGAMAVDHLLERLAEGRVVITPGDRADALLALIAAQGAAGFPTLAGV
                     IINGGFYPTDAVRRLLAGMHSSLPIIATDLGTFEAATICSHTRGRLTRDSGLKIDTAR
                     ALFEKHVDCDALRRIFNVASTDVVTPLMFEYALLDRARKNKRHIVLPEGDDDRILQAA
                     STLLRRGVVDLTILGTEATIRSRAAEL"
BASE COUNT          266 a          595 c          517 g          271 t
ORIGIN      
        1 ggccaatggg cgaggaagaa cgcgacgtag ggaagggtcc ctaggctcgc gtacaccatg
       61 aactggttcg tggcgataac ggagcgacat tccccccttg acaggggggg cacaccaatg
      121 tggctcaccg ccgcccacac gcgtcggagg cgttcgcgcg cgtccctcat tgtcacgcct
      181 tctcaccacc cctggcggct tgcacagcac cccgcatcgc cgggtacccg cccccgagcc
      241 ctccaatcct agaggctgtt ggttccgcgc gtcaggtgag gcgggctccg ccccgcagcc
      301 acacgcggca aaccgggttc tttgccccga ttttctggcg acccgggacc gttgggcacc
      361 tccggggccc tcggcagtga gatgctatct ccgtgagtcg tagcatctac attgcctccg
      421 cagagggcga ttgcggaaag tcgaccgtgg cactcggcgt cgtggaccac ctttcgcgaa
      481 ccatcgacaa ggtcggaatc ttccgccccg tcgcacgcgt caacgatggg tcggactatg
      541 tcctccaact cttgctcggg cacatcggcg tcgacctgag ctacgacgag tgcgtcgggg
      601 tcacgtacga cgacgttcac cgggatccgg atgccgcgct ctcgaccatc gtgtcgaggt
      661 accacgaggt ggaacgcaag tgcgacgccg tggtgatcgt cggaacggac tacaccgacc
      721 tcgcggcggc ggccgagttc gaatacaacg cgaggatcgc ggcgaacctg ggctcccccg
      781 tggccctggt cgttcgcggc gccgggcgct cccccgccga ggtccagcag gccatcgagt
      841 ccgcgcgcct cgaactcgag agcagccacg cgcgcctcgc ggccgtcttc gtcaacaagg
      901 cgaaccctga tgacgtcgag gagatccggg ccgcccttcc ggccggcctc ggcatgggcg
      961 tccttccgga ggatcccatc cttgtcgccc cgctcctcag cgagctcatg accgcggtgg
     1021 gcggcgaact gtattccggc gacgccaagc tcctcggccg agaggttctg ggagtgatca
     1081 tcggcgccat ggccgtcgac cacctgctcg aacggctcgc cgagggccgc gtcgtcatca
     1141 cgccaggaga cagggccgat gcccttctcg cgttgatcgc cgcgcagggc gcggcagggt
     1201 tcccgacgct ggccggggtc atcatcaacg gcggcttcta ccccaccgat gccgtgcgcc
     1261 gcctcctcgc ggggatgcac tccagcctcc cgatcatcgc gacggatctg ggaaccttcg
     1321 aggccgcgac catctgctcg cacacccgcg ggcgcctcac gcgcgactcg gggctcaaga
     1381 tcgacaccgc ccgcgccctg ttcgagaagc atgtggactg cgatgcgttg cgccggatct
     1441 tcaacgtcgc gtcgacggac gttgtcacac ccctgatgtt cgagtacgcg cttctcgatc
     1501 gcgcccgcaa gaacaagcgc cacatcgtcc ttcccgaggg cgacgacgac cggattctcc
     1561 aggccgcgtc gaccctgctc agacgcggag tggtggatct caccatcctc ggaaccgagg
     1621 ccaccatccg ctcccgtgcg gccgagctc
//
LOCUS       DASVKP010000024         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_583216, whole genome shotgun sequence.
ACCESSION   DASVKP010000024 DASVKP010000000
VERSION     DASVKP010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583216"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VF362_00470"
     CDS             complement(<1..372)
                     /locus_tag="VF362_00470"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7589436.1"
                     /translation="MKVLVTGGAGFIGSNFVRLTLDERPDVDVTVLDALTYAGSAASL
                     PASDRVRLVEGNIADAAVVDDLVRDADVVVHFAAESHNDNSLDNPWPFVQTNLVGTFT
                     LLEAVRRHGTRLHHISTDEVYG"
     gene            complement(369..980)
                     /gene="rfbC"
                     /locus_tag="VF362_00475"
     CDS             complement(369..980)
                     /gene="rfbC"
                     /locus_tag="VF362_00475"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX7589437.1"
                     /translation="MEFRPLAIEGAWEITPRQFADRRGVFLESYNEGPFAEAIGHRLA
                     VAQTNVSVSAAGVVRGIHFADVPPGQAKYVTCVSGAVWDVVIDIRVGSPTFGEWEGVV
                     LDDVNRRAVYLSEGLGHAFMSLEEGSAVTYLCSVPYNPGHEREIHPLDPEIGIEWPTR
                     GRDGSPIEPQLSGKDAAGPTLSEAREAGLLPTAEAVRLWRNAL"
     gene            1037..1906
                     /gene="rfbD"
                     /locus_tag="VF362_00480"
     CDS             1037..1906
                     /gene="rfbD"
                     /locus_tag="VF362_00480"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX7589438.1"
                     /translation="MEITEARWHIAGGYGMLATDIIAALRARGVEPVVTDRDDLDITN
                     PAKVARGFADADIVVNCAAYTAVDAAEEDEATAFAVNATGPGVIARACAEMGLRMVHF
                     STDYVFAGDATTPYAEDSPLGPKGAYGRTKAAGERQVRDSGADALVVRTAWLYGRGGP
                     CFPKTIARVGRERGALRVVDDQVGQPTWTRDLADLILRLVEAEAPAGTYHGTSSGQTS
                     WYEFAKEVVASAGLGDIVTPSSAADQKRRAPRPTYSVLGHDALSAIGLAAIGDWRERW
                     RLAAPEVLADVAT"
     gene            complement(1910..>2593)
                     /gene="rfbA"
                     /locus_tag="VF362_00485"
     CDS             complement(1910..>2593)
                     /gene="rfbA"
                     /locus_tag="VF362_00485"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEX7589439.1"
                     /translation="FQRLLGDGSQFGISISYATQSEPNGLAQAFVIGADFVGGDSAAL
                     VLGDNIFYGPGLGHTLQRFSDISGAAVFAYRVADPQAYGVVEFDAEGKALSLEEKPQH
                     PKSHYAVPGLYFYDNDVVAIARDLKPSARGEYEITDVNRVYLEAGTLQVEVLPRGTAW
                     LDTGTFDSLLDASQFVQTVERRQGMKIGSPEEVAWRRGFLTDDELRERAERLAKSGYG
                     HYLLRLLDE"
BASE COUNT          421 a          895 c          904 g          373 t
ORIGIN      
        1 gccgtagacc tcgtcggtgg agatgtggtg aagccgcgtg ccgtggcgac ggaccgcttc
       61 gaggagcgtg aaggtgccga ccaggttcgt ctggacaaac ggccacgggt tgtcgagcga
      121 gttgtcgttg tgggattccg ccgcgaagtg gacgaccacg tcggcgtcgc gcaccaagtc
      181 atcgacaacc gccgcatccg caatgttgcc ctcgacgagg cggacgcgat cgctcgccgg
      241 gagcgaggcg gcgctgcccg cgtaggtcaa tgcgtcgagg accgtgacgt cgacgtcggg
      301 ccgctcgtcg agagtgagcc gaacgaagtt ggagccgatg aacccggcac cgccggtcac
      361 gagaaccttc acagggcgtt cctccagagt cggaccgcct cggcggtcgg aagcaggcca
      421 gcctcgcggg cctcggacaa tgtggggccg gcggcgtcct tgccggacag ctgaggctcg
      481 atcggggaac cgtcgcgccc gcgcgtcggc cactcgatcc cgatctcggg gtcgagaggg
      541 tgaatctctc gttcgtgccc ggggttgtag gggaccgagc acaggtacgt gaccgccgag
      601 ccctcctcaa gcgacatgaa cgcgtgcccc aggccctcgc tgaggtagac cgcgcggcgg
      661 ttgacgtcgt cgaggaccac gccctcccac tcgccgaagg tgggcgagcc aacccggata
      721 tcgatgacga cgtcccacac cgcgccgctc acgcacgtga cgtacttcgc ctggccgggg
      781 ggaacatccg caaagtgaat gccccgcacc acgcccgccg ccgacaccga cacgttcgtc
      841 tgggccaccg cgagtcggtg gccgatggct tccgcgaagg ggccctcgtt gtacgactcg
      901 aggaacacgc cccgacggtc ggcgaattgc cggggcgtga tctcccacgc gccctcgatg
      961 gccaatggcc ggaactccac gatgcctccg ctcgatgatc ggacaccaga ctaccgttgc
     1021 cgctacgctg gcccgcatgg agatcaccga ggcgcggtgg cacattgcgg gcgggtacgg
     1081 aatgctcgcg acggacatca tcgcggcgct acgtgcgcgg ggggtcgagc ccgtcgtgac
     1141 cgaccgcgac gacctcgaca tcaccaaccc ggccaaggtc gcgcgcggat ttgccgacgc
     1201 cgacatcgtg gtcaactgcg ccgcctacac cgctgtcgac gccgccgagg aggacgaggc
     1261 gacggccttc gccgtcaacg cgaccgggcc gggcgtgatc gccagggcat gcgccgagat
     1321 ggggttgcgg atggttcact tctcgaccga ctacgtcttc gcgggggacg cgaccacccc
     1381 gtacgccgag gactccccgc tcggccccaa gggcgcctac gggcgcacca aggcggccgg
     1441 cgagaggcag gtccgcgact cgggcgccga cgccctcgtt gtccggaccg cgtggctcta
     1501 cggccgtggc ggtccgtgct ttcccaagac catcgcgcgc gtcgggcgcg agcgcggcgc
     1561 cctccgggtg gtggacgacc aagtcggcca accgacgtgg acgcgggacc tcgccgatct
     1621 gatcctccgc ctcgtcgagg ccgaggcgcc cgctgggacg taccacggga cgtcgtcggg
     1681 gcagacatcg tggtacgagt tcgccaagga ggtcgtggca tctgcgggac tcggggacat
     1741 cgtcacgccg agcagcgcgg cggaccagaa gcggcgagca cctcgaccca cgtactcggt
     1801 gctcggccac gacgcgctct ccgcgatcgg gctcgccgcg atcggggact ggcgcgagcg
     1861 ctggcgcctc gcggcccccg aggtcctggc cgacgtcgcg acctaggacc tactcgtcga
     1921 gcagccgcag caggtagtgt ccgtagccgg acttggccag gcgctccgcg cgctcgcgca
     1981 actcgtcgtc ggtgaggaag cccctccgcc acgcaacctc ctcgggcgag ccaatcttca
     2041 tcccctgccg acgctcgacc gtctggacaa actgagacgc gtcgaggagc gaatcgaagg
     2101 ttcccgtgtc caaccacgcg gttccccgcg ggagcacctc cacctgcagc gtgcccgcct
     2161 cgaggtagac gcggttgacg tccgtgatct cgtactcgcc gcgcgcggac ggcttgaggt
     2221 cgcgggcgat cgcgaccacg tcgttgtcgt agaagtacag gccgggcacc gcgtagtggg
     2281 actttgggtg ctgcggcttc tcttcgagcg atagcgcctt gccctccgcg tcgaactcga
     2341 ccacgccgta cgcctgaggg tccgcgacgc gatacgcgaa gacggcagcg ccggagatgt
     2401 ccgagaagcg ctgaagcgtg tgcccaagcc cggggccgta gaagatgttg tcgccgagga
     2461 cgagggccgc agagtcgccg ccgacgaagt ccgccccgat gacgaacgcc tgggcaaggc
     2521 cgttcggctc gctctgggtg gcgtagctga tcgagatgcc gaactgcgaa ccgtcgccga
     2581 gcagccgctg gaa
//
LOCUS       DASVKP010000025         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1241019, whole genome shotgun sequence.
ACCESSION   DASVKP010000025 DASVKP010000000
VERSION     DASVKP010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241019"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VF362_00490"
     CDS             <1..422
                     /locus_tag="VF362_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017822533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX7589440.1"
                     /translation="SSVRLGVADVVADVVSTGGTLRAAGLEIFGDPILKSEAILIRRP
                     DTPESAVATLTGRLRGVLMARQYVLVDYDVPAAALEAAIAITPGYEAPTVTALHDSEW
                     SAVRALVPRGEMNHVMDQLYAAGARAILVTELLACRV"
     gene            422..1417
                     /locus_tag="VF362_00495"
     CDS             422..1417
                     /locus_tag="VF362_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX7589441.1"
                     /translation="MPPSDAAERLLNLIIALSHARGRMTRAEIRATVSGYDAERGGDT
                     AERARRRTSAFERMFERDKEDLRRIGIPLRTVVDATHGDDIGYVIDAGEASMPPIDMT
                     PVERAILAVAAEYWQNAALGTDARRAFVKVSSSMPRAEEVPLHLAARSTEGSDAAPAL
                     AQAIADGQAVAFTYTSAASGRGERTVEPWRLVVRGGRLYVLGRDRDRGEARTYRLSRI
                     EGAIRPVGERGAFEPPDEVEVLALEGEVPTLMARVGLRAESGHALRRRASFVSTAGEW
                     DVFDVPFSDTEALRDEILSLGGAARVLEPEDLATAVRAHAEAALAIASAEGGGRG"
     gene            1419..>2290
                     /locus_tag="VF362_00500"
     CDS             1419..>2290
                     /locus_tag="VF362_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX7589442.1"
                     /translation="MAVPRLTRLLGIVTHLEEHGEATFDELAQHFGVTAETIKRDIET
                     LWCAGLPGYGMTDLLDFDGFAYDEGIARLTNSQGVRQVRLAPAEAMALMGALAAMIAS
                     GTAPEAATGALAALSAAVTDANPVTVIRAAPVNPGIVEALRAGIERTRAVAVTYVDAQ
                     DRRTSRVIEPHRLVTVDGLGYVECYCRRAGDYRTLRLDRIEVAGRTDDAATALPRAAS
                     GFTLEPAYSATVRVARAGRWAFEDLPGAVLAVDGNDVVATFGVANPDVVVARLLAVAP
                     HVGSVEPASLRERLG"
BASE COUNT          316 a          816 c          844 g          314 t
ORIGIN      
        1 aatcgtccgt gcggctcggc gtcgccgacg tggtcgcgga cgtcgtctcg accggaggaa
       61 cgctccgcgc cgcgggcctc gagatcttcg gcgaccccat cctcaagtcc gaggcgatcc
      121 taattcgccg ccccgacacg cccgagtcgg cggtcgccac gctgacgggc cgcctccgcg
      181 gcgtgctcat ggcgcgccag tacgtgctcg tcgactacga cgttccggcg gcggcgctcg
      241 aggcagcgat cgcgatcacg cccggatatg aggcgcccac cgtgaccgcg cttcacgact
      301 cggagtggtc ggccgtgcgc gcgctcgttc cccggggcga gatgaaccac gtgatggacc
      361 agctgtacgc cgcgggagcc cgcgcgatcc tcgtgaccga gctgctcgcc tgcagggtgt
      421 agtgccaccg tcggatgccg ccgagcggct cctcaacctc atcatcgcgc tgagccacgc
      481 ccgcggaagg atgacgaggg cggagatccg cgcgaccgtc tcggggtacg acgcggagcg
      541 cgggggcgac accgcggaac gggcgcgcag gcgcacctcc gcgttcgagc ggatgttcga
      601 gcgcgacaag gaggatcttc gccgcatcgg catccccctg aggacggtcg tcgatgcgac
      661 ccacggcgac gacatcggct acgtcatcga cgccggggag gcctcgatgc ccccgatcga
      721 catgaccccc gtggaacggg ccatcctcgc cgtcgccgcc gagtactggc agaacgcggc
      781 gctcggaacg gacgcacggc gcgcgttcgt gaaggtctcg tcgtcgatgc cgcgagcgga
      841 ggaggttccc ctccacctgg ccgcacgatc caccgagggg tccgatgcgg cccccgcact
      901 cgcccaggcg atcgcggacg ggcaggccgt ggcgttcacg tacacgtccg cggcctcagg
      961 tcgaggcgag cggaccgtcg agccgtggcg cctcgtggtt cggggcgggc gcctctacgt
     1021 cctgggccgc gaccgcgatc ggggggaggc gcggacctac cggctgagcc ggatcgaggg
     1081 cgcgatacgg ccggtggggg agcggggcgc gttcgagccg ccggacgagg tcgaggtgct
     1141 cgcgctcgag ggcgaggttc cgaccctgat ggcgcgcgtc ggccttcgcg cggagtcggg
     1201 ccacgcgctg aggcgccgcg cctcgttcgt ttccacggcg ggggagtggg acgtcttcga
     1261 tgtgccgttc agcgacaccg aggcgctccg cgatgagatt ctctcgctcg ggggcgccgc
     1321 ccgcgtgctc gagcccgagg acctcgcgac cgccgtccgg gcccacgcgg aggccgcgct
     1381 tgccatcgcc agcgccgagg gaggcggccg tggctgagat ggccgtaccg cggctgacgc
     1441 gcctgctcgg catcgtcacg cacctcgagg agcacggcga ggcgaccttc gacgagctcg
     1501 cgcaacactt tggcgtcacc gccgaaacga tcaagcgcga catcgagacc ctgtggtgcg
     1561 cgggcctgcc cggctacggg atgaccgacc tattggattt cgatggcttc gcgtacgacg
     1621 aggggatcgc gcggctcacg aactcccagg gagtgcgcca ggtgcggctc gccccggccg
     1681 aggcgatggc cctgatgggg gccctcgccg cgatgatcgc gtcgggaacc gcccccgagg
     1741 ccgcgacggg tgccctcgcg gccctcagcg cggccgtgac ggacgcgaac ccggtcacgg
     1801 tcattcgggc agcccccgtg aacccaggca tcgtagaggc gctcagggcc gggatcgagc
     1861 ggacgcgcgc ggtggccgtc acctacgtcg acgcccagga tcgtcgcacg tctcgcgtga
     1921 tagaacccca ccgactcgtc acggtggacg gccttggcta cgtcgagtgc tactgcagga
     1981 gggcgggcga ctaccgcacc ctgcggctcg accggatcga ggtggcggga cgaaccgacg
     2041 acgccgcgac cgcgctcccg cgggcggcga gcgggttcac gctcgagccc gcgtactccg
     2101 cgaccgtgcg cgtcgcgcgc gcgggcaggt gggccttcga ggaccttccc ggcgcggtgc
     2161 tcgcggtcga cgggaacgac gtggtggcaa cgttcggcgt cgcgaacccc gatgttgtcg
     2221 tcgcccgcct cttggccgtc gcgccccatg ttggctccgt cgaaccggcg agcctccggg
     2281 agcgcctcgg
//
LOCUS       DASVKP010000026         4549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_254744, whole genome shotgun sequence.
ACCESSION   DASVKP010000026 DASVKP010000000
VERSION     DASVKP010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254744"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VF362_00505"
     CDS             complement(<1..691)
                     /locus_tag="VF362_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7589443.1"
                     /translation="MRPEVHIPSVGMPLAAPPSVGDVLIELGLVLLVLALLGRLAQRI
                     GISSIPLYLLGGLALGKGGIMPIRAGDDFIAVGAEIGVVLLLLLLGLEYSPEDLRDGL
                     RSTWRAGVIDVVANALPGFAAGLILGWTPTASLLLAGITYISSSGIIARLLSDMDRIG
                     NRETPVILSLLVMEDVITAFVLPVIAVLLVGATLAQGALAAGLAIGVVLLAFYVAFRL
                     SARVSRAIHSRS"
     gene            complement(695..1174)
                     /locus_tag="VF362_00510"
     CDS             complement(695..1174)
                     /locus_tag="VF362_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX7589444.1"
                     /translation="MEIFETKIPGIGVRYEFTTEAGDRAGVVIRRDGTRDIVVYDPED
                     ADAAIGTIELSEGDAAAIAELLGGTTITVRLEQLRHDVEGLAIDWVVMPETGGLTDLT
                     IADGKIRTRTSASVVAVVRGDHAVPGPGPSFEFLAGDQVLVMGDADAVAEATRILTG"
     gene            complement(1349..2443)
                     /locus_tag="VF362_00515"
     CDS             complement(1349..2443)
                     /locus_tag="VF362_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7589445.1"
                     /translation="MTTTLRARLLASHLIVAAVGAGALVIVAAIVTNVAFRGRLHDGM
                     GMMGGPQGKGNPTTNQLQSALSESLAWALVIGVVAAVLTAGVAAIFVARRISQPVEQI
                     RAATHRMARGEFRSEIPLPADAELAELARDVNALGARLAESELHRSRLIGEVAHELRT
                     PLTTIRASMEGLIDGVVAPDPKVFARIAEEASRLHRLADDLTLLSQAEESAIPLRPVA
                     IDLGTVAASASARLAAQFEDKGVALVVSDFPPLPVRADPDRVTQILTNLLGNAMTHTP
                     EGGSITVSGGQADGNAWIAVADTGSGIPADQVDRVFERFYRVPDASHPAGRGIGLTIA
                     RGMARAHGGDVTATSDGIGKGTTFRLTLPA"
     gene            complement(2440..3141)
                     /locus_tag="VF362_00520"
     CDS             complement(2440..3141)
                     /locus_tag="VF362_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7589446.1"
                     /translation="MNDVAPPRILVVDDEENIRALVETYLKNDGYDVVTAATGEDAVE
                     MVASAAPDLVILDVRLPGIDGFEVLRRIRKQSDVFVIMLTARADELDTLVGLEVGADD
                     YVTKPFSPRELVARVRAMLRRTRGEPRIDQALHFDGLSVDEARREVLVDGAPAPLTAL
                     EFDLLGALASAPGRVFTRRQLIERVWGWDFYGEERVVDVHVRNLRKALGDPADSPRFI
                     STVRGVGYKLTARPR"
     gene            complement(3213..3566)
                     /locus_tag="VF362_00525"
     CDS             complement(3213..3566)
                     /locus_tag="VF362_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589447.1"
                     /translation="MTVSNKVWAIIGGAAALVIVPAGVTFALVAAHDEFRPGITERAP
                     ARAGQQGDSGMMQGGMFGGSGRAGNRDRGQGRDGSYCDGDGPQGRATDNADRGRGMMG
                     GYGQGMMDGSGARNR"
     gene            3683..4009
                     /locus_tag="VF362_00530"
     CDS             3683..4009
                     /locus_tag="VF362_00530"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX7589448.1"
                     /translation="MATSKDTVAHVLEQLEPLDVRARAMFGEYGVYCDEKIVALVCDD
                     TFFLKPSPATASLGIHLVAAPPYPGAKEYFVIGAAIVEDDEQFRRVVQATADALPAPK
                     PKPKRE"
     gene            4072..>4549
                     /locus_tag="VF362_00535"
     CDS             4072..>4549
                     /locus_tag="VF362_00535"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX7589449.1"
                     /translation="MRAARVLAIGSLADIIVEDVPVPVPDEDQLLVKVDFAALNPIDY
                     KVALHGHRGGREFPLTLGFDAVGTVAAVGSAVSSVAVGDRVCLMTDIMAPGTAAECVV
                     ARERNVAVVPAAIGDTQAAGLPLAGLTASQALDLAGLDAGQSVLIHAGAGGVGHLAI"
BASE COUNT          715 a         1523 c         1606 g          705 t
ORIGIN      
        1 gcgaacgcga gtggatcgcc cgcgacacgc gagcgctcag tcggaaggcg acgtagaagg
       61 cgagaaggac gacgccgatc gcgagtcccg cggcaagcgc gccctgcgcg agcgtcgccc
      121 cgaccagcag gacggcaatc acgggcaaga cgaaggccgt gatgacgtcc tccatcacga
      181 gaaggctgag gatgacgggc gtctcgcggt tcccgatccg gtccatgtcg ctgagaagtc
      241 gggcgatgat gccggacgac gaaatgtagg tgatccccgc gaggaggaga gacgccgtcg
      301 gcgtccaacc gaggatcagt ccggccgcga agccgggaag cgcgttcgcc acgacatcaa
      361 tgacgcccgc ccgccacgtc gatcgaagtc cgtcgcgcag atcctcgggc gaatactcga
      421 gcccaaggag caggaggagg aggacgactc cgatctcggc tccgaccgcg atgaagtcgt
      481 ctccggccct aatgggcatg atgccgccct tgccgagggc gagtccgccg agcaggtaca
      541 ggggaatgga ggagatgcca atgcgctgcg cgagccgacc gaggagggcc aggaccaaca
      601 ggacgagccc gagttcgatg aggacgtcgc ccaccgacgg cggagccgca agcggcatgc
      661 cgacggaggg gatgtgcacc tcgggccgca cgccctagcc cgtcagtatt cgcgtggctt
      721 cggcgaccgc atccgcatcc cccatgacca gcacctggtc cccggccagg aattcgaaac
      781 tcggtcccgg tcccggcacg gcatgatccc cgcggacgac ggcgacgacg gacgcggacg
      841 tgcgcgtccg gatcttcccg tcggcgatcg tcaggtcggt gagcccgccg gtttccggca
      901 tcaccaccca atcgatggcg aggccctcga cgtcgtggcg cagttgctcc agcctgacgg
      961 tgatggtggt gccgccgagg agttcggcga tcgctgcggc atctccctcg gagagctcga
     1021 tcgttccgat ggccgcgtcg gcgtcttcgg ggtcatagac gacgatgtcg cgcgtgccgt
     1081 cccgccggat cacgacgccc gcgcggtcgc ccgcttcggt cgtgaactcg taccggacgc
     1141 cgatcccggg aatcttggtc tcgaagatct ccatgcgtcg agcatggcac ggtcggcgaa
     1201 acggggtcta gatgtggcgg tgaagatgcg agcgttccgc gtcctggagc tcctcgaggc
     1261 gctgtggcac gacaagtcct gcgccgtgga cgccgcgctc gtcgccgtgc gcgctgccat
     1321 gaggggcggc gatgagcgcg ttgcgcgcct aggcgggaag cgtcagccgg aacgtcgtgc
     1381 ccttgccgat accgtcggag gttgcggtga cgtccccgcc gtgggcccgc gccatgccgc
     1441 gcgcgatcgt caggccgatt cccctgcccg ccgggtgcga ggcgtcggga acccggtaga
     1501 acctctcgaa gacgcggtcc acctgatctg cggggattcc gctcccggtg tccgcgacgg
     1561 cgatccatgc gttcccgtcg gcctggcccc cgctcaccgt gatggacccc ccctcagggg
     1621 tgtgcgtcat ggcgttgccc aacagattcg tgaggatctg cgtcacccgg tcgggatccg
     1681 cgcgaacggg aaggggtggg aagtcggaga cgacgagcgc gacccccttg tcctcgaatt
     1741 gggccgcgag gcgcgcggat gcggacgcgg cgaccgtgcc aagatcgatg gcgaccggcc
     1801 gaagcgggat cgcgctctcc tccgcctgcg agaggagggt gagatcgtcc gcgagccgat
     1861 ggagtcggga cgcctcctcc gcgatgcggg caaagacctt gggatcgggc gcgacgacgc
     1921 catcaatcag gccctccatc gacgcccgga tggtggtgag cggcgtgcgc agttcgtgcg
     1981 cgacctctcc gatgagccgg ctgcggtgga gctcgctctc ggcgagccgg gcgccgaggg
     2041 cattcacgtc ccgcgcgagt tccgcgagtt cggcgtcggc gggcagggga atctcgctgc
     2101 ggaattcgcc cctggccatc cggtgggtcg ctgcccgaat ctgctcgacg ggctgcgaga
     2161 tgcggcgcgc gacgaagatc gcggcgacac cggcggtgag gacggcggcg accacgccga
     2221 tcacgagggc ccaggcgagg gactcggaca gcgcgctctg aagttggttg gtggtggggt
     2281 ttcccttccc ttgcggcccg cccatcatgc ccattccgtc gtggagtcga cctcggaagg
     2341 caacattggt gacgatcgcc gcgacgatga cgagggctcc cgcgccgacc gcggcaacga
     2401 tgaggtgcga ggcgaggagg cgagcgcgca gggtggtggt caccgcggtc gcgccgtgag
     2461 cttgtaaccg accccgcgaa cggtgctgat gaagcgagga gagtcggcgg ggtcgccgag
     2521 cgccttgcgg agattgcgaa cgtgcacgtc cacgacgcgc tcctccccgt agaagtccca
     2581 gccccacact cgctcgatga gttggcgtcg ggtgaagacc cgcccgggcg ccgacgccag
     2641 ggcgccgagc aggtcgaatt cgagggccgt caggggtgcg ggcgcgccgt cgacgagcac
     2701 ctcccgtcgt gcctcgtcga cgctgagccc gtcgaagtga agcgcctggt cgatccgggg
     2761 ttcgcccctg gtccgtcgca gcattgctcg aacacgtgcg acgagctcgc gcgggctgaa
     2821 gggtttggtc acgtagtcat cggcgcccac ctcgaggccg accagggtgt ccagttcgtc
     2881 ggcgcgcgcc gtgagcatga tgacgaacac gtccgactgc ttccgaatcc ggcgtaggac
     2941 ttcgaagccg tcgatgcccg gcaggcgaac gtcgaggatc accaggtccg gcgcggcgct
     3001 cgcaaccatc tcaaccgcgt cctcacccgt ggccgccgtg acgacgtcgt agccgtcgtt
     3061 cttgaggtag gtctcgacga gcgcccggat gttctcttcg tcgtcgacga ccagaatccg
     3121 cggaggggcc acgtcattca tgataggtcg gtcccccgga tgcggactgg cgcggtgtcc
     3181 tcgtggggca ccgcgccatg gtccgactca cctcagcggt tccgggcgcc ggaaccgtcc
     3241 atcattccct gtccgtaacc gcccatcatg ccgcgacccc ggtcggcgtt gtcggtggct
     3301 cgcccttggg ggccgtcgcc gtcgcagtag gacccgtcgc gcccttggcc ccgatcgcgg
     3361 tttccggcgc ggccggaacc gccgaacatc ccgccctgca tcatccctga atcgccctgc
     3421 tggcccgcgc gtgcgggggc gcgctcggta atccccgggc ggaactcgtc gtgcgcggcg
     3481 accagggcga acgtgacgcc cgccgggacg ataaccaatg cggccgcgcc gccgatgatg
     3541 gcccatacct tgttgctgac cgtcatgaca tgcctctcta gtcgggtgaa gcgatgcctc
     3601 cattccaccc gccttgcatg gagggacgag gttccgaacc gtgaagatcg tatgaagcgt
     3661 cggcggggcc tagcctggag ccgtggcaac atcgaaggac acggtggcgc acgtgctcga
     3721 gcaactcgag cctctcgacg ttcgcgcgcg cgccatgttc ggcgagtacg gcgtctactg
     3781 cgacgagaag atcgtggcgc tcgtctgcga cgacaccttc ttcctcaagc cctcgcccgc
     3841 gacggcgagt ctggggatcc acctggtggc cgcgccccca taccccgggg cgaaggagta
     3901 cttcgtgatc ggggcggcga tcgtcgagga cgacgagcag ttccgacggg tcgtccaggc
     3961 gacggccgac gccctcccgg cgccgaaacc caagccgaag agggaatagg acgggacaaa
     4021 ggctccacgc caggggcccg ccgggacgta cggtgtgggg tggaggaaat catgcgcgct
     4081 gcccgtgtgc tcgctatcgg ttctttggcc gacatcatcg tggaggacgt tcccgttccc
     4141 gtgccggacg aggatcagct cctggtcaag gtggacttcg ccgctctcaa tcccatcgac
     4201 tacaaggtcg cgctgcacgg tcatcgcgga gggcgcgagt ttcctctcac cctcgggttc
     4261 gacgcggtcg gaacggttgc ggccgtcggc tccgcggtct cgtcggttgc cgtgggcgat
     4321 cgcgtctgcc tcatgacgga catcatggcg ccgggtacgg cggccgagtg cgtggtcgcg
     4381 cgcgagcgca acgtcgcggt cgtcccagcc gcgataggcg acacgcaggc ggcgggactg
     4441 cccctcgcag ggcttaccgc ctctcaggcc ctcgacctcg ccggcctcga tgccggccaa
     4501 agcgtgctca ttcacgccgg cgccggtggt gtggggcatc tggcgatcc
//
LOCUS       DASVKP010000027         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_502045, whole genome shotgun sequence.
ACCESSION   DASVKP010000027 DASVKP010000000
VERSION     DASVKP010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502045"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1094)
                     /locus_tag="VF362_00540"
     CDS             complement(99..1094)
                     /locus_tag="VF362_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HEX7589450.1"
                     /translation="MNGTLAATTTHTIPFRGRTTALTLVLPGAIIAATLATAWAWRGS
                     MPDPIAVHWGTNGPDGFGSFAPNILWPEAVAAITAVLLWALGYFAGSQSAIRKSAAGI
                     AVWMAVLMSGVNFSIMSLQRGLTDAHAAGSINGPMAFVFLAATAAAIGAAVLSPSDPP
                     LPATEPVAPTAARLALRPGEHATWIREVTSRGYVGAVVAVVAGEAIIGLLSRAWGLAL
                     VLGLVIGLSLVVFLHWTVIVNDEGLTVRSALSWPRFVIPLDEVEMAEVVQVRPLPEFG
                     GWGVRGGKGGRVGVIIRKGPALQVHRSGGRVFLVTVDDAETGAALLNTLAARARG"
     gene            complement(1091..1441)
                     /locus_tag="VF362_00545"
     CDS             complement(1091..1441)
                     /locus_tag="VF362_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7589451.1"
                     /translation="MLFRLDPSGTESLSTQLASQIRSAAARGELAPGTRLPSARDLAA
                     SLGVNLHTVLKAYQDVRDEGLLELRRGRGAVMTDASRDVRRLREAIDGAVAIARELGV
                     SPTTTVALVKEALQ"
     gene            complement(1541..2158)
                     /locus_tag="VF362_00550"
     CDS             complement(1541..2158)
                     /locus_tag="VF362_00550"
                     /inference="COORDINATES: protein motif:HMM:NF017853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HEX7589452.1"
                     /translation="MTDINDLFAQLPIGEIAKELGVSKDEAAKGVALALPALVGGLHA
                     NASDPKGAASLLTALEGKDTSLVEGSISLADVDTADGTKIVKHIFGAATPDVAAKLGG
                     ASALGSGMMKKLLPILAPIVLAFVVKQMTGSKAKTTTPSSGGLQDILGGILGSATGGG
                     SSSGGGIGDILGQVLGGAMSGGSSKASTGNAITDILGGLLGGGTK"
     gene            complement(2216..2695)
                     /locus_tag="VF362_00555"
     CDS             complement(2216..2695)
                     /locus_tag="VF362_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEX7589453.1"
                     /translation="MPAVAFFEPRIPENTGNAIRLSAVTGAALHLIEPLGFDLTEPRL
                     RRAGLDYHDLASVIVHASLDAMLAALPDSRVFAFTTRATTRFTDVAYEASDVLLFGPE
                     PTGLPDEVLAHPRITAQVTIPMRPGRRSLNLANSAAIAAYEAWRQLGFVGDARAPSA"
     gene            complement(2775..3188)
                     /locus_tag="VF362_00560"
     CDS             complement(2775..3188)
                     /locus_tag="VF362_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589454.1"
                     /translation="MDDRPSRADGMTEENVPSDEIPIPAILLAPKNGSAVERVRVRNT
                     NWKGVVGIVVAVVVAPNFAALASSAPAGAPFAVALAIALATPLVAILAGIRMAFDIRR
                     GAESSKGLAVAAIVTGMGMSALMIIALVLFSAFAH"
     gene            complement(3216..3452)
                     /locus_tag="VF362_00565"
     CDS             complement(3216..3452)
                     /locus_tag="VF362_00565"
                     /inference="COORDINATES: protein motif:HMM:NF039500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5680 domain-containing protein"
                     /protein_id="HEX7589455.1"
                     /translation="MIRASDTPTARAGAVIKESLAALYRQGRFLGGHVYTVGEDVYTD
                     TNAGDVAHFTGTEWITRRGERVYQLAYDGGLVKE"
     gene            3406..>3507
                     /locus_tag="VF362_00570"
     CDS             3406..>3507
                     /locus_tag="VF362_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7589456.1"
                     /translation="MTAPARAVGVSDARIIFRHILPNSLSPIIVSATL"
BASE COUNT          521 a         1177 c         1302 g          507 t
ORIGIN      
        1 tggtcgcggc cttcaacgac gcgggcgcgg gtccgcgctc gggcgtggtg tgcgccagcc
       61 cgcagtgatc ggccgccggg cctagccgcg cacgcacgtc agccccgcgc gcgcgccgcg
      121 agcgtgttga gaagcgcggc gccggtctcg gcgtcgtcca ccgtgaccag gaagactcgg
      181 ccgcccgacc ggtgcacctg cagcgcgggg cccttgcgga tgatcacgcc gacccgcccg
      241 cccttgccgc cgcgtacgcc ccagcccccg aactccggca ggggccgcac ctgaaccacc
      301 tcggccatct ccacctcgtc gagcgggatg acgaaccgcg gccagctgag cgccgagcgc
      361 accgtgaggc cctcgtcgtt gacgatcacg gtccagtgca ggaagaccac gagcgacagt
      421 ccgatcacca ggccgaggac gagggcgagg ccccatgcgc gggagagaag cccgatgatg
      481 gcttcgccag ccacgacggc gaccaccgcg ccgacgtagc cgcgggacgt gacctcccgg
      541 atccacgtgg catgctcgcc gggccgaagc gcgaggcggg cggccgtcgg agccacgggc
      601 tcggtcgcgg gcagcggcgg gtcggagggg ctgaggacgg cggcgccgat cgccgccgcc
      661 gtggccgcga ggaacacgaa cgccatcggg ccgttgatcg accccgcggc gtgcgcgtcg
      721 gtgagtcccc gctgcaggct catgatcgag aagttgaccc cggacatgag cacggccatc
      781 cacacggcga tccccgccgc gctcttgcgt atggcggact ggctccccgc gaagtacccg
      841 agcgcccaaa gcaacacggc cgtgatcgcc gcgacggcct cgggccacag gatgttcgga
      901 gcgaacgagc cgaagccgtc gggcccgttg gtgccccagt gaaccgcgat cggatccggc
      961 atgctcccgc gccacgccca tgcggtggcg agcgtcgccg cgatgatcgc gccggggagg
     1021 acgagggtga gggcggtggt gcggccacgg aagggaatgg tgtgcgtggt ggtcgcggca
     1081 agggttccgt tcactgcaat gcctccttaa cgagggcgac ggtggtggtg ggggaaacgc
     1141 cgagctctcg cgcgatggcg acggcgccgt caatggcttc gcgcaggcgc ctgacgtcgc
     1201 gcgaggcgtc ggtcatgacg gcgccccgac cccggcgaag ttcgaggagc ccctcgtcgc
     1261 ggacgtcctg gtacgccttg aggaccgtgt gaaggttgac gccgagcgat gccgcgaggt
     1321 cgcgcgcgga gggcaggcgc gtcccgggtg ccaactcgcc cctcgcggcg gccgagcgga
     1381 tttggctcgc gagttgcgtc gagaggcttt cggttcccga ggggtccagg cgaaagagca
     1441 taggtccacg atacatgttc taggagaact agaacaacag ggcaaggcaa gcggggcgcg
     1501 acatccttcg ccgcgccccg gcaggggcag tgcaagggga ctacttggtt ccgccgccga
     1561 gtagtccgcc gagaatgtca gtgatcgcgt tgcccgtgct ggccttcgag gaaccgccgc
     1621 tcatcgcgcc gccgaggacc tggccgagga tgtcgccgat gccgcccccg gagcttgagc
     1681 cgccgcccgt cgcgctgccg aggatcccgc ccaggatgtc ctggaggccg ccggagctcg
     1741 gggtcgtcgt cttggccttc gagccagtca tctgcttgac gacgaacgcc aggacgatcg
     1801 gcgcgaggat cgggaggagc ttcttcatca tgcccgagcc gagcgcgctc gcgccgccga
     1861 gcttggccgc gacgtcgggg gtcgcggcgc cgaagatgtg cttgacgatc ttggtgccat
     1921 cggccgtgtc gacgtcggcg agcgagatgc tgccttcgac gaggctcgtg tccttgccct
     1981 caagcgcggt gagcagggac gcggcgccct tggggtccga cgcgtttgcg tgcaggccgc
     2041 cgacgagcgc gggaagggca agggcgacgc ccttggccgc ctcgtccttg ctcacgccaa
     2101 gctccttggc gatctcgccg atggggagtt gggcgaagag gtcgttgatg tcggtcacgg
     2161 gtactccttg gtcgttgggc gcggtcgggc gggcctgatt cggtgactca aaaccctacg
     2221 cgctcggagc ccgcgcgtcg cccacgaacc ccaactgccg ccacgcctcg taggccgcga
     2281 tggcggcgga gttcgccagg ttgagcgagc gccgacccgg gcgcatgggg atggtcacct
     2341 gcgccgtgat tcgggggtgg gcgaggacct cgtcgggcag gcccgtcggc tcggggccga
     2401 acagcaggac gtcggacgcc tcgtacgcga cgtccgtgaa gcgcgtcgtg gcgcgggtgg
     2461 tgaaggcgaa gacgcgcgaa tccgggagcg cggcgagcat cgcgtcgagg ctcgcgtgca
     2521 cgatgaccga cgcgaggtcg tggtagtcga ggccggcgcg cctcaggcgc ggttcggtca
     2581 gatcgaagcc gagcggctcg atgagatgca gggccgcgcc cgtgacggcc gagagccgga
     2641 tggcgttgcc cgtgttctcc gggattcgcg gctcgaagaa cgcgacggcg gggatgcgcg
     2701 ggtcggcggg cggggggctc ggcggggcgg acgcggcgtc ggtcacgcgc ccattgtgtc
     2761 agtgggcgat tgtgtcagtg ggcgaaggcg gagaagagaa ccagggcgat gatcatgagg
     2821 gcgctcatcc ccatgcccgt gacgatggcc gcgaccgcga ggcccttgct cgactccgcg
     2881 ccacgccgga tatcgaacgc catgcgaatt ccggcgagga tggccacgag gggagtggcc
     2941 agcgcgatgg cgagggccac cgcgaacggc gcgccggccg gggccgacga ggccagcgcc
     3001 gcgaagttcg gggcgaccac gaccgcgacg acgatcccga cgacgccctt ccagttcgtg
     3061 ttgcgcacgc ggacgcgctc caccgcggac ccgttcttgg gggcaagcag gatggcggga
     3121 atggggatct cgtcgctcgg gacgttctcc tcggtcatcc cgtcagccct gctgggtcga
     3181 tcgtccatct tcgaagttta gtggttcatc cacggctact ccttgacgag cccgccgtcg
     3241 taggccaact ggtacacgcg ctcgccgcgc ctggtgatcc attccgttcc ggtgaagtgc
     3301 gcgacgtcgc ccgcgttcgt gtccgtgtag acgtcctcgc cgacggtgta cacgtgcccg
     3361 ccgagaaagc gcccctgccg atagagcgcc gcgaggctct ccttgatgac cgcgcccgcc
     3421 cgcgccgttg gcgtgtcgga cgcccggatc atctttcgcc acatcctgcc gaactcgctg
     3481 tcgccgatca tcgtctcggc gaccctg
//
LOCUS       DASVKP010000028         1779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_584882, whole genome shotgun sequence.
ACCESSION   DASVKP010000028 DASVKP010000000
VERSION     DASVKP010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584882"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VF362_00575"
     CDS             complement(<1..1179)
                     /locus_tag="VF362_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589457.1"
                     /translation="MVDAGDARERVMQCPACGYTIRLSTDRVCGACGIDATHPALAEI
                     READATLYGYKATYDELIAKWQELSQRRYGLLATLIGTRGSAVAEPAATAAPATAAPA
                     TSGTFFPAPDAAAVSAADATTAPALAADLAFAPVASAPIPGVPPFDNQGLADRFPTLP
                     PRPDRPARPVRPVRATPRRLTAPALLGVSGASLLITSAIVFIAVTWQTFFPLAQGLII
                     SAVAAATAYLSLWLKRHDLSVSGGAVGVVAMSFVGVAIVAVDRQAGVLGAFALPVAAA
                     FTAGAGLALSRAGVVWVGSMAALALGASAAGLTRAIAVQPRVDDRLAWALAGPALALA
                     VLATFRLWATAPSRLILRVAGVALLTLAPVPLLLDIAWNGRSSAFALALVLPLGALIA
                     L"
     gene            1295..1762
                     /locus_tag="VF362_00580"
     CDS             1295..1762
                     /locus_tag="VF362_00580"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7589458.1"
                     /translation="MTTQLSRGDLAPDFTLPTDEGPFALADQRGKHVIVYFYPAAGTP
                     GCTTQACDFRDNLASLQAAGYVVVGVSPDEPPALAKFRASEHLTFPLASDPERTVFDA
                     YGAFGEKSLYGKTVTGVLRSTVVIDPEGKVELAQYNVKATGHVAKLRKDLGLD"
BASE COUNT          304 a          602 c          667 g          206 t
ORIGIN      
        1 gagggcgatc agggcgccca ggggtagcac cagcgcgagc gcgaaagcgg aggagcgccc
       61 gttccaggcg atgtcaagca gaagcggcac gggagcgagg gtcaggagcg ccacccccgc
      121 cacgcgaaga atgaggcgcg acggcgccgt cgcccacaac cggaacgtgg cgaggaccgc
      181 cagggcaagg gcgggtcccg caagagccca cgcgagccgg tcgtcgacgc gcggctggac
      241 cgcgatcgct cgggtaaggc ccgcggccga ggcaccgaga gcgagcgcgg ccatcgagcc
      301 cacccacacg acgccggcgc gggacagggc gagcccggcg cccgccgtga acgcggccgc
      361 gacgggcagc gcgaaggcgc caagaacgcc cgcctgccgg tcgacggcga cgatcgccac
      421 cccgacaaac gacatcgcga cgacgccgac ggcgccgccc gagacggaga gatcgtggcg
      481 cttgagccag agcgaaaggt acgccgtggc cgccgcgacc gcgctgatga tcaggccctg
      541 cgcgaggggg aagaaggtct gccacgtcac cgcgatgaag acgatcgccg aggtgatgag
      601 gagcgaggcg ccggaaacgc cgagcagcgc gggcgcagtg agccgccgcg gggtcgccct
      661 gacggggcga acgggtcggg cggggcgatc cggtcgagga ggtagcgtcg ggaacctgtc
      721 tgcgagaccc tggttgtcga acgggggcac ccccggaatg ggggcgctgg ccaccggcgc
      781 gaaggcgaga tcggcggcca gcgctggggc cgtcgtcgcg tccgcggcgg agacggccgc
      841 tgcatcgggt gcggggaaga acgttccgga ggttgcgggg gctgcagtgg cgggggcagc
      901 ggttgcggca ggctcggcga cggccgaacc ccgcgtaccg atcagggtcg cgagcaggcc
      961 gtagcggcgc tggctcagtt cctgccactt cgcgatgagt tcgtcgtacg tggccttgta
     1021 gccgtagagc gtggcgtccg cctcgcggat ctccgcgagc gccgggtgcg tggcgtcgat
     1081 gccacaagcg ccgcacacgc ggtccgtcga gagtcgaatg gtgtacccgc acgcggggca
     1141 ctgcatgacg cgctcgcgag catcccccgc atccaccatc agaaggcccc gtcccgtcgt
     1201 tacgctgacc gtaccgacac tcggggtgcg gtgcaacgcg agcgatcatc ggaggggggg
     1261 gaatgcgccg ccgcacccgc tcgttgtgcc tcacatgacg acacaactat cgcgcggcga
     1321 cctcgccccc gacttcaccc ttccgaccga cgaggggccc ttcgcgctgg ccgatcagcg
     1381 gggcaagcac gtgatcgtgt acttctatcc ggccgcggga acgcccggat gcaccacgca
     1441 ggcgtgcgac ttccgcgaca acctggcctc gcttcaggcg gcgggatacg tcgtggtcgg
     1501 cgtctccccc gacgagccgc ccgcgctcgc gaagttccgg gcgagcgagc acctgacctt
     1561 ccccctcgcg tcggaccccg aaaggaccgt cttcgacgcg tatggggcgt tcggcgagaa
     1621 gagcctctac ggcaagaccg tgacgggcgt gctccgctcg accgtcgtga tcgatcccga
     1681 gggcaaggtc gaactcgcgc agtacaacgt gaaggcgacg ggtcacgtcg cgaaactccg
     1741 gaaggacctg gggctcgatt gacctgggcc gactgacct
//
LOCUS       DASVKP010000029         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1077577, whole genome shotgun sequence.
ACCESSION   DASVKP010000029 DASVKP010000000
VERSION     DASVKP010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077577"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VF362_00585"
     CDS             complement(<1..253)
                     /locus_tag="VF362_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589459.1"
                     /translation="MDDDAIESDEASARAGRARREPIPATGAWREGDEPGDRRFFDLG
                     SLPLDVASNGADPRGVDPSGVLPGVRLAYETWGELNADCS"
     gene            complement(261..1574)
                     /locus_tag="VF362_00590"
     CDS             complement(261..1574)
                     /locus_tag="VF362_00590"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional o-acetylhomoserine/o-acetylserine
                     sulfhydrylase"
                     /protein_id="HEX7589460.1"
                     /translation="MSSDNWSFETKQIHAGQTPDAETGARALPIYQTTSFVFESTKQA
                     ADRFALAELGPIYTRLGNPTQEVVEARVAALEGGVGALLVASGQAAETITILNLAEAG
                     SHIVASPSLYGGTYNLLHYTLPKYGIDVTFVDDPDDLKQWKAAIRPNTKALFAESISN
                     PKQDVLDIEGVAKVAHEAGIPLVVDNTVATPYLVQPLKWGADFVIHSATKYLGGHGTA
                     IGGVIVDGGTFDFAKQPERFPQYNTPDPSYHGLTYAKDLGVGGIFGVNLAFILKARVQ
                     LLRDLGPAVSPFNAWLIAQGLETLSLRVERHVENAGKVAAWLEARPEVASVAYASLPS
                     SKWHKNATKYAPKGAGAVLAFEIAGGADKARAFIDALELHSLVANIGDVRSLVIHPAT
                     TTHSQLTEAEQATTGVTPGTVRLAVGIENIDDILADLELGFAAIK"
     gene            1892..2455
                     /locus_tag="VF362_00595"
     CDS             1892..2455
                     /locus_tag="VF362_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX7589461.1"
                     /translation="MSSARLRTAVAMIAATTVLSACGGLAPAATATPATPSTATPSTA
                     TQSPATLPDAATFARALFDGTNATRSDAGIPQLVWSECAAELALPRALHTLPEGILSH
                     EPLLPSCEDFTYLGENLARANYTPAQVMAAWLDSPGHRANLLDPDFTEVGIGCVPYEA
                     SSPSVPASGSSDAGGMACSEVFLGYVP"
     gene            complement(2443..>2824)
                     /locus_tag="VF362_00600"
     CDS             complement(2443..>2824)
                     /locus_tag="VF362_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589462.1"
                     /translation="DGLDGSAHTARQLTPGMARDADIIIAMSAEDRAAIVRDESSSIP
                     KTLLLMEVAAAAGANVPLAGATRKKRVANIPSSIIACRPELSGMVWEDVPDPYGRGPK
                     DYERAYLMIKEAVLAIDRWVTQGT"
BASE COUNT          414 a          952 c          972 g          486 t
ORIGIN      
        1 tgctgcagtc cgcgttcagt tctccccacg tctcataggc gagtcgcacg ccggggagga
       61 cgccgcttgg atcgacgccg cgcggatcgg ccccgttgga ggcaacatcg agcggcaagg
      121 acccaaggtc gaagaatcgg cggtcgccgg gttcatctcc ctccctccac gcacccgtgg
      181 cgggaatcgg ctctcgccgt gcccgccccg cgcgcgcgga ggcctcgtcg ctctcgatcg
      241 cgtcgtcgtc cacggcgggc ctacttgatc gccgcgaacc cgagctcgag gtcggcgagg
      301 atgtcgtcga tgttctcgat gcccacggcg agccgcacgg ttcctggagt gacacccgtc
      361 gtcgcctgct cggcctcggt cagctgggag tgcgtggtcg tcgcggggtg gatgacgagc
      421 gagcgcacgt ccccgatgtt ggccacgagc gagtgaagtt cgagcgcgtc gatgaacgcg
      481 cgcgccttgt ccgccccgcc agcgatctcg aacgcgagca ccgcgcccgc gcccttcggc
      541 gcgtacttgg tcgcgttctt gtgccacttc gacgacggca ggctcgcgta cgcgacgctc
      601 gccacttccg gtcgcgcctc gagccacgcg gccaccttgc ccgcgttctc cacgtgacgc
      661 tccacgcgga gcgacagagt ctcgagaccc tgggcgatga gccacgcatt gaacggcgag
      721 accgccggtc cgaggtcccg caggagctga acgcgcgcct tgagaatgaa ggcgaggttc
      781 accccgaaga tcccgccgac gccgaggtcc ttggcgtagg tgaggccgtg atagctcggg
      841 tcgggggtgt tgtactgcgg gaagcgctcg ggctgcttgg cgaagtcgaa cgtgccgccg
      901 tcgacgatca cgccgccgat cgcggtcccg tgcccgccaa ggtacttggt cgccgagtgg
      961 atgacgaagt ccgcgcccca tttgagcggc tgaacgaggt acggcgtcgc gacggtgttg
     1021 tcgacgacga gcgggattcc cgcctcgtgc gcgaccttcg cgacgccctc gatgtccagg
     1081 acatcctgct tggggttgga gatggactcc gcgaacagcg ccttcgtgtt gggtcggatc
     1141 gcggccttcc actgcttcag gtcgtcgggg tcgtccacga acgtgacgtc gatgccgtat
     1201 ttgggcagcg tgtagtgaag gaggttgtac gtcccgccgt agagcgacgg agaggcgacg
     1261 atgtgcgagc cagcctcggc gaggttaagg atcgtgatcg tctcggcggc ctggccggac
     1321 gcgacgagaa gcgctccgac cccgccctcg agcgccgcga cgcgcgcctc gaccacctct
     1381 tgggtggggt tgccgaggcg ggtgtagatg ggcccgagct ccgcgagcgc gaagcggtcc
     1441 gccgcctgct tggtgctctc gaagacgaac gacgtcgtct ggtagatcgg gagggctcgc
     1501 gcccccgtct ctgcatccgg cgtctggccg gcgtggatct gcttggtctc gaaggaccag
     1561 ttgtcgctac tcatgttgct tcctcttcct ggggtgtgat ggggggaaac tcaatcccgg
     1621 tcacaggaag ggagggcagc acgccgaccc gccagcccat gacagggcct gcggttggca
     1681 cgttgcgggg gtgtgcccgt cagggggctg ctggcctatg tcggcccagc ggggcgccct
     1741 tcggggcgca cgtcgaacgt gcgctggtta gctgcacatt cgacgggaca tgtccgtgag
     1801 cgtaaggcat gccttagccc tagcgcaaat cctgacgcgg tccggcatcg cggcggggtg
     1861 gctagggtgg actctgagtg aaggaggagc gatgtcaagc gcccggttgc gcacggccgt
     1921 cgcgatgatc gccgcgacga cggtcctgtc ggcatgcggt ggtctcgccc ccgccgccac
     1981 cgcgaccccc gcgaccccgt ccaccgcgac cccgtccacc gcgacccagt cccccgcgac
     2041 ccttcccgac gcggccacct tcgcgcgagc gctcttcgac gggacgaacg cgacgcggag
     2101 cgacgccggg atacctcaac tcgtgtggtc ggagtgcgcc gcggaacttg cgttgccgcg
     2161 cgcgctacac acgttgcccg agggcattct gagccatgaa ccgctcctgc cgtcgtgtga
     2221 ggacttcacc tacctggggg aaaacctcgc tcgcgccaac tacaccccgg cccaggtcat
     2281 ggccgcgtgg cttgactcac cggggcatcg ggcgaacttg ctcgatcccg acttcacgga
     2341 ggtcggcatc ggctgcgtac cgtacgaagc ctcgagcccg tcggtcccgg cctccggctc
     2401 ctcggatgcg ggcgggatgg cgtgttccga ggtgttcctc ggctacgtcc cctgagtcac
     2461 ccatctgtcg atggcgagca cggcttcctt gatcatcagg taggcccgct catagtcctt
     2521 gggaccgcgc ccatagggat cgggcacgtc ctcccacacc atgcctgaca actcgggccg
     2581 acaggcgatg atcgaagacg gaatgttcgc gacccgtttc ttgcgcgtgg cgcccgcgag
     2641 gggaacattc gccccggccg cggccgccac ctccatgagg aggagcgtct tagggatgga
     2701 cgacgattcg tcacggacga tcgccgcacg gtcctcggcg ctcatcgcga tgatgatgtc
     2761 ggcgtctcgc gccatgccgg gggtgagttg ccgtgccgtg tgcgcggaac cgtccaggcc
     2821 gtca
//
LOCUS       DASVKP010000030         7303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1488899, whole genome shotgun sequence.
ACCESSION   DASVKP010000030 DASVKP010000000
VERSION     DASVKP010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488899"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VF362_00605"
     CDS             complement(<1..332)
                     /locus_tag="VF362_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7589463.1"
                     /translation="MRILLRLAWLEAKLLLREPITVVFTLGLPVVMELVMGGVFGNRA
                     DPAYYRGVGAMDYYMGAYVGLVVAALGLITLPTHIATYRERGVLRRFDASTISRSTVI
                     GAQTIVMIG"
     gene            complement(329..1336)
                     /locus_tag="VF362_00610"
     CDS             complement(329..1336)
                     /locus_tag="VF362_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7589464.1"
                     /translation="MPTPPIDVVLAVEDLHKNYGATRAVDGVSFEVRRGEIFGILGPN
                     GAGKTTTVECLQGLRRADSGTIRILGLDPTRDLAVVKRRIGSQLQESALPDRIKVWEA
                     LDLFSASVDGAAPWESLIDDWGLRAKRNAQFGSLSGGQKQRLLVALALVNRPEVVFLD
                     EMTTGMDPVARRVAWGLIERIRDDGATVVLVTHFMDEAERLCDRAAIIQAGKVVAIGT
                     PDELRSAHGNTVTVTFTGDEKDRALAAKVRGVESATIDETAAVVSPSAGAASTEGRIE
                     ARGRGPIGVHIAAALAKAGREPADYAVTRPTLEDVYLAITGDVDADEDTDRHAAKGAT
                     P"
     gene            1505..2806
                     /locus_tag="VF362_00615"
     CDS             1505..2806
                     /locus_tag="VF362_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7589465.1"
                     /translation="MTSPADVVSIAQDLIRIDTTNPGDGTGPGERAATEYVATFLSNL
                     GLDPVIRESEPRRTCMTALWEGEDRSRPPLVVHGHLDVVKAFPEEWRHPPFAAEVHEG
                     MIWGRGAVDMKGTDAVYLATVANMIGAGIKPARDIVLGFMADEEAGGLKGARFMVDHH
                     RDDFRGATEAISEVGGFSTQIDGRRAYLLQTAEKGLLWLRLVATGTAGHGSLVHEDNA
                     VTHLAEALAKVGKYAWPLDITPTVEALLRGVADLTGDTYEPTPEVVDRLIAALGSAAR
                     FVEPTVRNTSNPTQIDGGYKANVIPNTATGVVDTRPLYGQHEEVVAVISELAGPNVKV
                     EPLVSDIALEVPFSGPLVDAMIASIPSEDPGAPVLPYALSGGTDNKHMSRIGITGYGF
                     APLQLPPDLDFPSLFHGVNERVPVAGLEFGVRVMERFLRTC"
     gene            complement(2844..3101)
                     /locus_tag="VF362_00620"
     CDS             complement(2844..3101)
                     /locus_tag="VF362_00620"
                     /inference="COORDINATES: protein motif:HMM:NF040150.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 family protein"
                     /protein_id="HEX7589466.1"
                     /translation="MHEISTQSISGAVVPSGRTPPRSSRVETREVVIPATVSRTDARQ
                     MLADQAEYGKWELARVLLYRGGARRVWLRRRAMKVRRSDED"
     gene            3143..3499
                     /locus_tag="VF362_00625"
     CDS             3143..3499
                     /locus_tag="VF362_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein"
                     /protein_id="HEX7589467.1"
                     /translation="MEAEVREALRHLITAFENHLEAVASRRSPDDAAVDDAYEALAAA
                     FERYEEKLDLEFAEGLPLVLDDVDDGDDDSDFEYDEGDDEDDDESDGVVVVDALGDDA
                     EEDEIDDDLDEFNLRD"
     gene            complement(3572..4531)
                     /locus_tag="VF362_00630"
     CDS             complement(3572..4531)
                     /locus_tag="VF362_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7589468.1"
                     /translation="MERRRAGSTGLEVSRLALGTMTWSRDTDDVEAGLQLDAFLDAGG
                     TLLDTAASYADGGAEELIGSMLGGTISRRDVQICTKAGIRRTDAGRTVDASRDSLLGT
                     LDESLERLGTDYVDVWLVHAFDPVTPLEETVHALEIAVNSGRARYVGISNFPGWATAR
                     AATLLPRPGLAVAEVEYSLAQRGIEREVVPASRALGLGIFAWSPAGRGVLTGKYRTAI
                     PADSRAASDHLAGFVEPYLTDRVAAIVEAIVTAAAGLGRTTHEVALAWVRDAPGVTSA
                     IVGARTSEQLAPVLSAEDLTLPGAIREALDEISAPSMGYPERR"
     gene            4596..5456
                     /locus_tag="VF362_00635"
     CDS             4596..5456
                     /locus_tag="VF362_00635"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX7589469.1"
                     /translation="MNWWEAAVLGVVQGLTEFLPISSSAHIRVVGALLPNGSDPGAAF
                     TAVIQIGTVAAVLWYFRRDIAQILGAWFRALAGRDGKDRASRFGAKSADARLGWWVIL
                     GSIPIIVLGLLFQKSIESSLRNLWITVGVLAAFALVLAWADKIGAKRRELGSLRARDA
                     VVLGLAQAAALIPGVSRSGGTISAGLFLGLSRIAAARYSFLLAIPAVLGSGVYELFSN
                     RAELTEPGKPGLTNTVIAAVVSAGVGYAVIVWFLKMLTTRSFAPFVVYRWIFAAAIVV
                     LILTNTIPVR"
     gene            complement(5499..6368)
                     /locus_tag="VF362_00640"
     CDS             complement(5499..6368)
                     /locus_tag="VF362_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX7589470.1"
                     /translation="MALPDRVAPLDRDATIMIAAFEGWNDAGSSASAAVDHLAAVWAA
                     KEYLSLDAEDFHDFQVSRPAVSRNERGERVVSWPGTVISTAEIPSQPGRTVLIARGIE
                     PSMRWRQFCSEVLEAAEDFDVTVLVTLGALLVDAPHSRPLPIYLSSEQLEMRKLLDIE
                     RSDYDGPTGITGVLGHEAESRGISVVSGWVGVPHYVAHPPSPKATRALLGVVERLVGA
                     PIDLGDLEYEAEAWERGVDELASEDEEIAAHVKTLEEVTDAANLPEASGDAIAAEFER
                     FLRRNEGGAARQL"
     gene            6393..7079
                     /locus_tag="VF362_00645"
     CDS             6393..7079
                     /locus_tag="VF362_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX7589471.1"
                     /translation="MDENGGWPDAILWDMDGTLIDTEPYWIGAEIALCASHGASWTHE
                     DGLSLVGNPLVTSAEVLRERGVDLPIPEIIDALLDEVIARVTDHVPWQRDARELLLRA
                     RSGGVPCALVTMSYASLADAFIGAAGAFESVVTGDFVVNGKPHPEPYLTAAAELGVPI
                     ERCLAIEDSPSGIRSAHASGARTVAVRRLTPLEPLPGMSRVRSLDSLTDDVIGAIMGG
                     RVLDELGDAV"
     gene            complement(7091..>7303)
                     /locus_tag="VF362_00650"
     CDS             complement(7091..>7303)
                     /locus_tag="VF362_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEX7589472.1"
                     /translation="TEQDLDQIEHEVRALWNDIVAAATTSTWRTRRTALCGWCNFQAL
                     CPEFGGTPPALDPAEVEAALGVTPRA"
BASE COUNT         1059 a         2545 c         2555 g         1144 t
ORIGIN      
        1 ccgatcatca cgatggtctg cgcgccgatc acggtcgaac gcgagatggt cgacgcgtcg
       61 aatcgacgca ggacgccccg ttcgcgatac gtcgcgatgt gagtcggcag cgtgatgagg
      121 cctagggccg ccacgacgag ccccacgtag gctcccatgt agtagtccat cgctccgacg
      181 ccgcggtagt aggcggggtc cgccctgttt ccgaacacgc cgcccatgac gagttccatg
      241 acgaccggta gcccgagggt gaagacgacg gtgatgggct cgcggaggag caacttggcc
      301 tcgagccagg cgaggcgcag caggattctc acggcgtcgc ccccttcgcc gcgtggcggt
      361 ccgtgtcttc gtccgcgtcg acgtcacccg tgatcgcgag gtagacatcc tcgagggtcg
      421 gtcgcgtcac ggcgtagtct gcgggctcgc ggccagcctt cgcgagcgcg gccgcgatgt
      481 gcactccgat cggcccgcgc ccccgcgcct cgatcctgcc ctccgtggat gcagccccgg
      541 cgctcggaga tacgacggcc gcggtctcgt cgatcgtcgc gctctccaca cctcggacct
      601 tggcggcgag cgcgcggtcc ttctcgtcgc cggtgaacgt gacggtcacc gtgttgccgt
      661 gcgcgctccg caactcatcc ggggtgccga tcgcgacgac cttgcccgcc tggatgatcg
      721 cggcgcggtc gcacagtcgc tcggcctcgt ccatgaagtg ggtgaccagg accaccgtgg
      781 cgccgtcatc gcgaatccgc tcgatgagcc cccacgcgac tcgtcgcgcg acggggtcca
      841 tgccggtggt catctcgtcc aggaacacga cctcggggcg gttcaccagg gcgagggcga
      901 cgaggagccg ctgcttctgg ccaccgctca gggaaccgaa ctgggcgttg cgcttggcgc
      961 ggaggcccca gtcgtcgatg aggctctccc agggagccgc cccgtcgacg ctcgcggaga
     1021 agagatcgag ggcctcccac accttgatgc ggtcggggag cgcggactcc tggagctggc
     1081 tgccgatgcg gcgcttcacc acggcgagat cgcgcgtggg atcgaggccg aggatgcgga
     1141 tggtcccgga gtcggcgcgc cggaggcctt ggaggcattc cacggtggtc gtcttcccgg
     1201 cgccattggg gccgagaatg ccgaagatct ctccgcgccg gacctcgaac gagacgccgt
     1261 cgaccgcgcg ggtcgcgcca tagttcttgt ggaggtcctc cacagcgaga acgacgtcga
     1321 tgggcggcgt cggcatcggt ccctcccctt cccctgaggg cccccgggcc cgttgcgccc
     1381 agcgtagggc ccgcgaccgg gcgacgcgag gacgaatggc ccgacgtccc cgggctcgac
     1441 gccccatggc ccgaagccac ggcctcgacg ccgccgggcc agacccttgg tctagcctgg
     1501 tggcgtgacc tcaccagccg acgtcgtctc catcgctcag gacctcatta ggatcgatac
     1561 cacgaacccg ggcgacggaa cggggcccgg cgagcgcgcc gcgaccgagt acgtcgcgac
     1621 cttcctgtcg aacctcgggc tcgaccccgt catccgcgag tccgagcccc gccgcacctg
     1681 catgaccgcg ctgtgggagg gcgaggaccg ctcgcgccct ccgctcgtcg tccacgggca
     1741 cctcgacgtc gtcaaggcgt ttcccgagga atggcggcac cccccgttcg ctgccgaggt
     1801 ccacgaaggg atgatctggg gccgcggcgc cgtggacatg aaggggacgg acgcggtgta
     1861 cctcgcgacc gtggcgaaca tgatcggcgc ggggatcaag cccgcccgcg acatcgtgct
     1921 cggcttcatg gcggacgagg aggcgggcgg cctcaagggt gcccgcttca tggtcgacca
     1981 ccaccgcgac gacttccgcg gcgcgaccga ggccatcagc gaggtgggcg gattctcgac
     2041 gcagatcgat gggcgcaggg cctacctcct ccagaccgcg gagaagggcc tcctatggct
     2101 acggctcgtc gcgacgggca cggccggcca tgggagtctc gtgcacgagg acaacgccgt
     2161 gacccacctg gccgaggccc tcgcgaaggt ggggaagtac gcgtggcccc tcgacatcac
     2221 gcccaccgtt gaggcgctcc tgcgcggcgt cgcggacctc acgggggaca cctacgagcc
     2281 gacccccgag gtcgtcgacc gcctcatcgc cgccctggga agcgcggccc ggttcgtcga
     2341 gcccacggtc cgcaacacct cgaaccccac ccagatcgac ggcggctaca aggcgaacgt
     2401 catccccaat accgcgacgg gcgtcgtcga cacgcgcccc ctatacgggc agcacgagga
     2461 ggtcgtcgcg gtcatctccg agctcgccgg tcccaacgtc aaggtcgagc ccctcgtgag
     2521 cgacatcgcg ctcgaggttc cgttctccgg gcccctcgtt gacgcgatga tcgcgtcgat
     2581 cccctcggag gaccccggcg cgccggtgct tccgtacgcg ctgagcggcg ggacggacaa
     2641 caagcacatg agccgcatcg ggatcacggg ctacgggttc gcgccgctcc agctcccgcc
     2701 cgacctggac ttcccttcgc tcttccacgg cgtcaatgag agggtgccgg tcgccgggct
     2761 cgagttcggg gtgcgcgtca tggaaaggtt cctgcggacc tgctgagcgg cgtcgggcgg
     2821 cttggcgccg ctatccctcg tcgctagtcc tcgtcgctgc ggcggacctt catcgcgcgc
     2881 ctgcgtagcc agacccgcct ggcgcccccg cggtacagga gcacgcgggc gagctcccac
     2941 tttccgtatt cggcctggtc ggcgagcatc tggcgggcgt cggtgcggct cacggtcgca
     3001 gggatgacga cctcgcgagt ctcgacgcga ctcgagcgcg ggggagtgcg cccggaggga
     3061 acgacggcgc cggatatcga ttgggttgag atctcgtgca tagcgttacc agtttgcacc
     3121 tctcaccggt aacgtgagag ccatggaagc cgaggtccgc gaggcgttgc gtcacctgat
     3181 taccgcgttc gagaaccacc tggaggccgt cgcctccaga aggagcccgg acgatgccgc
     3241 agtcgacgac gcctatgagg ccctcgcggc ggccttcgag cgctacgaag agaagttgga
     3301 tctggagttc gccgagggcc ttccgctcgt cctggacgac gtggacgatg gcgacgacga
     3361 ttccgacttc gagtatgacg agggcgacga cgaggacgac gacgagtccg atggcgtggt
     3421 tgtggtggac gcgttgggcg acgacgccga agaagacgag atcgacgacg atctggacga
     3481 gttcaacctt cgcgactagc gtcgcccccg ccgcacgacg cttcggccaa ctcggcgcgg
     3541 cctgcctctg cgcggcctac cttcgctccg cctacctccg ctctgggtag cccatcgacg
     3601 gcgccgagat ctcatcgagc gcctctcgaa tcgcgccggg aagcgtgagg tcctccgcgc
     3661 tcagtaccgg cgccagctgc tcggatgtcc gggcccccac gatggccgag gtcacgcccg
     3721 gcgcgtcgcg aacccaggcg agcgcgacct cgtgcgtcgt gcgcccgagt cccgccgccg
     3781 ccgtgacgat cgcctcgacg atcgcggcga ccctgtccgt caggtagggc tcgacgaatc
     3841 ccgcgaggtg atcggaggcc gcgcgggagt ccgcggggat ggccgtccgg tacttgcccg
     3901 tcagcacgcc ccggcccgcg ggagaccacg cgaagatccc gagcccgagc gcccgcgagg
     3961 cgggcacaac ctcgcgctcg atcccgcgct gcgcgaggga gtactccact tcggcgacgg
     4021 cgagccccgg ccgcgggagg agtgtcgcgg cgcgggctgt cgcccacccg gggaagttgc
     4081 tgatgccgac gtaccgggcg cgtcccgagt tgaccgcgat ttcgagggcg tggaccgtct
     4141 cctcgagcgg cgtgacggga tcgaaggcgt ggacgagcca gacgtccacg tagtcggtgc
     4201 cgaggcgctc gagggactcg tcgagggtcc cgaggagcga gtcgcgcgag gcgtcgaccg
     4261 ttcgcccggc gtcggtgcgg cggatcccgg ccttcgtgca gatctggacg tcgcgccgcg
     4321 agatcgtgcc cccgagcatc gagccgatga gttcctctgc gccgccgtcg gcgtacgagg
     4381 cggcggtatc gagtagcgtt ccgcccgcgt cgaggaaggc gtcgagttgg aggccggcct
     4441 cgacgtcgtc ggtatcccgg ctccaggtca tggtcccgag cgcgaggcgc gatacctcga
     4501 ggcccgtcga gcccgcgcgt cgtctctcca cggtccagac gctacccgcg gcgccgcgac
     4561 gggagcatgc gactcgcggc ggtagtgtgg cgagaatgaa ctggtgggag gccgcggtcc
     4621 tgggcgtagt acagggcctt accgaattct tgcccatctc ctcgagcgcg cacattcgcg
     4681 tcgtcggagc gctgctgccc aacgggtcgg acccgggtgc ggccttcacc gcggtcatcc
     4741 agattggcac ggttgccgcg gtcctctggt acttccggcg cgacatcgcg cagattctcg
     4801 gcgcgtggtt ccgtgccctg gccggtcggg acggcaagga ccgggcctcc cggttcggcg
     4861 ccaagagcgc cgacgcgcgg ctcggctggt gggtgatcct cgggtcgatc ccgatcatcg
     4921 tgctcggcct cctgtttcag aagtccatcg agtcgagcct gaggaacttg tggatcactg
     4981 tgggagtcct cgccgcgttc gcgctcgtcc ttgcgtgggc ggacaagatt ggggcgaagc
     5041 gccgcgagct ggggagcctg agggcgcgcg acgccgtcgt ccttggcctc gcgcaggcgg
     5101 cggcgcttat acctggcgtc tctcggtccg gcgggacgat ctccgccggg ctcttcctcg
     5161 ggctgagccg catcgcggcg gcgcgctact cgttcctgct cgccatcccc gcggtactcg
     5221 gctcgggcgt ctacgagctc ttcagcaaca gggccgagct gacggagccg ggcaagcccg
     5281 ggctgacgaa cacggtgatc gccgcggtcg tctcggctgg ggtcgggtat gcggtcatcg
     5341 tgtggttcct gaagatgctg acgacgcgct cgttcgcgcc gttcgtcgtg taccgatgga
     5401 tcttcgcggc ggccatcgtc gtgctgatcc tcaccaacac cattcctgtg cgctagaccg
     5461 ggaacggcgg ctggcgggga actgcggcct gcggagggct acagctggcg cgccgccccg
     5521 ccctcgttgc ggcgaaggaa ccgttcgaac tcggcggcga tggcgtcgcc ggacgcctcg
     5581 ggaaggtttg ccgcgtccgt gacctcttcg agcgtcttga cgtgcgccgc gatctcctcg
     5641 tcctcgctcg ccagttcgtc gacgccgcgt tcccacgcct ccgcctcgta ttcgaggtca
     5701 ccgaggtcga tcggggcgcc gacgaggcgt tccaccacgc cgagaagggc gcgcgtcgcc
     5761 ttggggctcg gcgggtgcgc gacgtagtgg gggaccccca cccagccgga gaccacggag
     5821 attccgcgcg actccgcctc atgtccgagc acgccggtga tgcccgtcgg cccgtcgtag
     5881 tcggagcgct cgatgtcgag aagcttgcgc atctcgagct gttccgacga gaggtagatg
     5941 ggaagcgggc gagagtgggg ggcgtccacc aggagcgcgc cgagcgtcac gagcacggtc
     6001 acgtcgaagt cctccgcggc ctcgaggacc tcgctgcaga actgccgcca ccgcatggag
     6061 ggctcgatcc cgcgggcaat caggacggtc cgcccgggct gcgacgggat ctcggccgtc
     6121 gagatcaccg ttcccggcca ggagacgacg cgctcgccgc gctcgtttcg ggagaccgct
     6181 gggcggctca cctggaagtc gtggaagtcc tcggcatcca ggctgaggta ctccttggca
     6241 gcccacacgg cggcgagatg gtcgaccgct gcggaggcgg aggagccggc gtcgttccag
     6301 ccctcgaacg cggcgatcat gatcgtcgca tcccggtcca gcggcgccac gcggtcgggg
     6361 agtgccatca cacgagccta gactgtgcgc cgatggatga gaacggtggc tggcccgacg
     6421 cgatcctgtg ggatatggac ggcacgctga tcgacaccga gccgtactgg atcggtgccg
     6481 agatcgcgct gtgtgcctcc cacggagcct cgtggaccca cgaggacggg ctttccctcg
     6541 tcggcaatcc gctcgtcacg tccgccgagg tcctccgcga acgcggagtc gatctgccca
     6601 tccctgagat catcgacgca ctgctcgacg aagtgatcgc ccgcgtgacc gaccacgtcc
     6661 cgtggcagag ggacgcgcgc gaactgctcc tgcgggcgcg gagcgggggc gtgccgtgcg
     6721 cgctcgtgac catgtcgtac gcgagcctcg ccgacgcctt catcggcgcg gcgggcgcgt
     6781 tcgaatcggt ggtcaccggg gatttcgtcg tgaacgggaa gcctcacccc gagccctacc
     6841 tgaccgcggc ggcggagctc ggggttccga tcgagcggtg cctcgccatc gaggattcgc
     6901 cctccgggat tcgctcggcc cacgcctccg gcgcgcgcac cgtcgcggtt cgtcgcctca
     6961 ccccgctcga acccctcccg ggaatgtcgc gggtgcgtag cctcgactcg ctcaccgacg
     7021 atgtgatcgg cgcgatcatg gggggccggg ttctcgacga actcggcgac gccgtctagc
     7081 ctgtcgacat ctacgcccgc ggcgtcacgc cgagcgcggc ctctacctcc gcgggatcga
     7141 gcgcgggcgg agtacccccg aactcggggc ataacgcctg gaagttgcac cagccgcaca
     7201 gcgcggtgcg cctcgtacgc caggtcgagg tggtggccgc agccacgatg tcgttccaga
     7261 gcgcccgcac ctcgtgttcg atctgatcga gatcctgctc cgt
//
LOCUS       DASVKP010000031         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_256058, whole genome shotgun sequence.
ACCESSION   DASVKP010000031 DASVKP010000000
VERSION     DASVKP010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256058"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..>1553
                     /locus_tag="VF362_00655"
     CDS             222..>1553
                     /locus_tag="VF362_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017200820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX7589473.1"
                     /translation="MFHKVLVANRSEIAVRAFRAAYELGLHTVAVFPHEDRMSEHRLK
                     ADEAYEIGEVGHPVRAYLDVDELVRVARLSGADAVYPGYGFLAENVAFAERCRDEGLV
                     FIGPPPEVLAKAGDKVAALKAARDAGIPVLPSTQPSKDPAELAAWASGIGFPIFVKAV
                     AGGGGRGMRRVETPAELPEALAAAMREAESAFGDPTVFLERAVTAPRHIEVQILADSH
                     GNVIHLFERDCSIQRRHQKVIEIAPAPNLDPAIRDALFRDAVKFAKSLGYVNAGTVEF
                     LVSTEGERAGQHVFIEMNPRIQVEHTVTEEVTGVDIVVAQMRIAQGESLADLGLTQDS
                     ITVTGYALQTRITTEDPSNGFVPDTGRITAYRSPGGAGVRLDGATGLAGGRVTGHFDS
                     LLVKLTCRGHDFKQAVTRSRRALAEFRIRGVTTNIPFLLAVLGDPDFQVGGV"
BASE COUNT          247 a          578 c          500 g          228 t
ORIGIN      
        1 gccctcggca aggtctggcg ggaactcgag ggttccctcg cctcgttccc tgtcgaccac
       61 cccagcagtc ttcccatcga ggcccccggc gccgtcgacg aactggcggc gcgtcgccaa
      121 actcgctccc acgcgagcta ggcggatcag gcactcattc cgcgtccgag cccaagaggc
      181 gccggacctg gcaaaatagg tccgagggcg aagggacgcg catgtttcac aaggtgctgg
      241 tggccaaccg gtcggagatc gccgtccgcg ccttccgcgc ggcctacgaa ctcggcctcc
      301 acaccgtcgc cgtcttccct catgaagacc gcatgtccga gcaccgactc aaggccgacg
      361 aggcatacga gatcggcgag gttgggcacc ccgtccgggc ctacctggac gtcgacgagc
      421 tcgtgcgcgt cgcgcgtctc tcgggggccg acgcggtcta ccccggctat gggttcctcg
      481 cggagaacgt cgcgttcgcc gagcgatgca gggacgaggg cctcgtgttc atcggcccgc
      541 ccccggaggt cctcgccaag gcgggagaca aggtggcagc cctcaaggcc gcgcgcgacg
      601 cggggatccc cgttctcccc tcgacccagc cctccaagga tcccgcagag ctcgccgcgt
      661 gggcctcggg catcggcttc cccatcttcg tcaaggccgt cgccggtggg ggagggcggg
      721 gcatgcgccg ggtcgagacg cccgccgaac tgcccgaggc tctcgccgcg gcgatgcgcg
      781 aggccgagag cgcgtttgga gacccgacgg tcttcctcga gcgcgccgtc acggcgcccc
      841 gccacatcga ggtccagatt ctcgccgatt cgcacggcaa cgtcatccac ctcttcgagc
      901 gcgactgctc gatccagcgc cgccaccaga aggtgatcga gatcgccccg gcgcccaacc
      961 ttgaccccgc catccgcgat gccctgttcc gcgacgccgt caagttcgcg aagagcctgg
     1021 gatacgtcaa cgcgggaacc gtggagttcc tggtttccac cgagggggag cgggcggggc
     1081 agcacgtctt catcgagatg aacccccgaa tccaggtgga gcacacggtg accgaggagg
     1141 tgaccggcgt cgatatcgtc gtcgcgcaga tgcgaatcgc gcaaggcgag agcctcgccg
     1201 accttggcct cacccaggat tcgatcaccg tcacggggta cgccctccag acccgcatca
     1261 cgaccgagga tccctccaac ggcttcgtcc cggacaccgg caggatcacc gcctaccgtt
     1321 cgcccggcgg cgcgggcgtc cgcctcgacg gcgcgaccgg actcgctggc gggcgcgtca
     1381 ccgggcactt cgactcgctc ctggtcaagc tcacatgccg gggccacgac ttcaagcagg
     1441 cggtgacccg ctcaaggcgc gccctcgcgg aattccgcat ccgcggcgtc accacgaaca
     1501 tccccttcct cctcgccgtc ctgggcgacc ccgacttcca ggtcggggga gtg
//
LOCUS       DASVKP010000032         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_503348, whole genome shotgun sequence.
ACCESSION   DASVKP010000032 DASVKP010000000
VERSION     DASVKP010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503348"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VF362_00660"
     CDS             <1..1046
                     /locus_tag="VF362_00660"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7589474.1"
                     /translation="AAIAAAKNPANHKYTPAGGMPDLKKAIAAKTLRDSGYEVDPAQI
                     VVTNGGKQAVFQAFASILDDGDEVILPTPYWTTYPEAIRLAGAHPVEVFAGPDQDYLV
                     TVEQLEAARTPKTKALLFCSPSNPTGAVYSPEQTKAIGEWALEHGIWVVTDEIYEHLV
                     YDDAVFTPIVKVVPELAETCIVVNGVAKTYAMTGWRVGWMIGPLDVIKAATNFQSHLT
                     SNVANVSQRAAIAALEGGLEDVALMRKAFDRRRHTMVEMLSAIDGIVCPTPKGAFYCY
                     PSVEGLIGREIRGKRPANSAELAALILEEVEVAVVPGEAFGPSGFLRLSYALSDEDLV
                     EGVGRMQRLFSGS"
     gene            1163..2026
                     /locus_tag="VF362_00665"
     CDS             1163..2026
                     /locus_tag="VF362_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7589475.1"
                     /translation="MNAPAVSIRGLQKHYGSARAVDGLDLDIAQGEIFALLGPNGAGK
                     TTTVEILEGYRKRSAGEVTVLGHDPSHAHRDWRERIGIVLQSSADETPLTAREYVAHV
                     GSFFPNPRDTAEVLESVGLTELADRRGKTMSGGERRRLDVALGIVGRPELLFLDEPTT
                     GFDPEARHDFWTLIEGLKRDGTTILLTTHYLEEAEHLADRVGVITHGKLVALDTPAGL
                     RGGMDGAIVSWREGGKRRERATPTPTALVRELAAKHAAADGEVPDLAIRRPTLEDRYL
                     ELVGHGGRNEE"
BASE COUNT          377 a          737 c          705 g          310 t
ORIGIN      
        1 aggccgcgat cgccgcggcg aagaaccccg cgaaccacaa gtacacgccc gcggggggca
       61 tgcccgacct caagaaggcg atcgcggcca agacgctgcg ggactcgggt tacgaggtcg
      121 accccgccca gatcgtcgtc accaacgggg gcaagcaggc ggtcttccag gcgttcgcgt
      181 cgatcctgga cgacggggac gaggtcatcc tcccgacgcc gtactggacc acgtacccag
      241 aggcgatccg cctcgcgggc gcgcaccctg tggaggtctt cgcgggaccc gatcaggact
      301 acctcgtcac ggtggagcaa ctcgaggcgg cccggacacc caagaccaag gcgttgctct
      361 tctgttcgcc atccaacccc accggcgcgg tctactcacc cgagcagacc aaggcgatcg
      421 gcgagtgggc gctcgaacac ggcatctggg tcgtcaccga cgagatctac gagcacctgg
      481 tctacgacga cgcggtattc accccaatcg tcaaggtcgt cccggaactc gccgagacgt
      541 gcatcgtggt caacggggtc gccaagacgt acgcgatgac cggatggcgc gtggggtgga
      601 tgatcggccc gctcgacgtg atcaaggcgg cgaccaactt ccagtcgcac ctcacctcaa
      661 acgtggcgaa cgtctcccag cgcgcggcga tcgccgcgct cgagggcggc ctcgaggacg
      721 tcgcgctcat gcgcaaggcc ttcgaccggc ggcgccacac gatggtggag atgctctccg
      781 ccatcgacgg gatcgtctgc cccaccccga agggcgcgtt ctactgttac cccagtgtcg
      841 aggggctgat cggtcgggag atccggggca agcgcccggc caactccgcc gagctcgccg
      901 cgctcatcct cgaagaggtc gaggtggccg tcgtcccggg tgaggccttc gggccgagcg
      961 ggttccttcg cctcagctat gcgctgagcg acgaggacct cgtcgagggc gtcggccgca
     1021 tgcagaggct gttctccggt tcctaggctt ctcgcggttc gggggcctcg ggtatcaccc
     1081 tgagggatga ccctccgcgt ccgcgaagat cggccgaagg aacgatcccc tcggccgacg
     1141 cacgttcgct agcctggggc ccatgaacgc accagcggtg agcattcgtg gtcttcagaa
     1201 gcattacggc agcgcgcgcg cggtcgacgg actcgacctc gacatcgcac agggcgagat
     1261 cttcgccctc ctgggaccaa acggcgctgg aaagacgacg accgtcgaaa tccttgaggg
     1321 ctatcggaag cgtagcgcgg gcgaggtgac ggttctcggc cacgacccga gtcacgcgca
     1381 ccgcgattgg cgcgagcgga tcggcatcgt gctccaatcg agcgccgacg agacgcccct
     1441 caccgcgcgc gaatacgtcg cgcatgtcgg aagcttcttc cccaatcccc gcgacaccgc
     1501 cgaggttctc gaatccgttg ggctcacgga actcgcggat cggcgcggca agaccatgtc
     1561 cggaggcgag cgccggaggc tcgacgtcgc cctcgggatc gtcgggcgac cggaactgct
     1621 cttcctagac gagccgacga cgggcttcga ccccgaggcg cgccacgact tctggacgct
     1681 catcgagggg ctcaagaggg acgggacgac catcctcctc accacccact acctggagga
     1741 ggccgagcac ctcgccgacc gcgtgggcgt catcacccac gggaaactcg tcgcgctcga
     1801 caccccggcc gggcttcgcg gaggaatgga cggcgcgatc gtgtcgtggc gagagggcgg
     1861 caaacgtcgc gagcgggcga cgcccacgcc caccgcactc gtgcgggagt tggccgcgaa
     1921 gcacgccgcg gcggacgggg aagtccccga cctcgccatt cgaaggccca cgctcgagga
     1981 tcgatacctc gaactcgtcg gccacggggg aaggaacgaa gaatgagcac ctccacgcgt
     2041 ccaggcttct ggcgcctctc cggagcgagg gcggccaccg agctccggat cttcttccgc
     2101 gagtgggaca gcctcatctt cgtcttctt
//
LOCUS       DASVKP010000033         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_750512, whole genome shotgun sequence.
ACCESSION   DASVKP010000033 DASVKP010000000
VERSION     DASVKP010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750512"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VF362_00670"
     CDS             complement(<1..1043)
                     /locus_tag="VF362_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HEX7589476.1"
                     /translation="MSALLPFVAGPLRSLLETPGVTDVLVNGPDEVWIDRGMGLERAS
                     VGFASAGDVRELAVRLASLGGRRLDDACPLVDARLPDGTRLHAALPPVVDGCAAISLR
                     TVRRGALTLDDLELCGTLAPGLAPVLAGLVAGRASLLITGATGSGKTTLLASLLSLVD
                     PSERIVVIEEAGEVNPTHPHVVRLVERRANVDGAGAVEVSRLVREALRMRPDRIVLGE
                     CRGAEVRDVLTALNTGHRGGMATLHANAPADVPARLAALGALAGMDARATDLHAAAAF
                     EAVIHLEKTSAGRRVSEIAILRAEEGRRSARLRVRAALRVGGDGRIEAGPAWPEIEAL
                     LPAASEGTLSEGT"
     gene            1208..2008
                     /locus_tag="VF362_00675"
     CDS             1208..2008
                     /locus_tag="VF362_00675"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7589477.1"
                     /translation="MTEHGSTAAFFDLDKTIIATSSTARFSRDLRSNGLLKTSDALRT
                     AYAQFLFMVGGADARQTARLRDALAETIKGWEAAKVRRIVGETVKQLIDPVVYEEALT
                     LIRRHQANGRDVVIVSASGAEVVQPIASLLGTKDFIASRMEVVDGVFTGKISRYVFGP
                     YKADAIRAMAAQRGYELERCYAYSDSITDAPMLDAVGYGFAVNPNPAMRRAAAAHGWG
                     VLHFRRPVALRESRRRRGIVVGATVAGVALTTALIWWGVTRRRDREIV"
     gene            2215..>2681
                     /locus_tag="VF362_00680"
     CDS             2215..>2681
                     /locus_tag="VF362_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7589478.1"
                     /translation="MVQDALVKVFSRLRPLPNVAAADQYVRRAMLTLYLDRTRRVTMW
                     RRLRGTLATDDAVAGPEDAAAAGTDVRAALAGLSPRERTCVVLRHFDDLSTRQVAEAT
                     GLAEGTVKRYLSDALAKHEGAGERPERHLELHRLSGLQRRRQGVRGHVHLRVA"
BASE COUNT          395 a         1010 c          931 g          345 t
ORIGIN      
        1 atggtcccct ccgacagggt cccctccgac gcggcgggaa ggagcgcctc gatctcgggc
       61 cacgccggac cggcctcgat gcggccgtcc ccgcccacgc gcagcgccgc cctgacgcgc
      121 agccgcgcgg agcggcgacc ctcctcggca cgaaggatcg cgatctcgga cacccggcgg
      181 cccgcggagg tcttctcgag gtgaatcacc gcctcgaagg ccgcggcggc gtgaaggtcc
      241 gtggcgcgcg cgtccatccc ggccagcgcg ccgagcgccg cgaggcgggc cggaacgtcc
      301 gcgggcgcgt tcgcgtggag ggtggccatg ccgccgcgat gtcccgtgtt gagtgccgtc
      361 agcacgtcgc gaacctcggc tcccctgcac tcgccgagca cgatccgatc cgggcgcatc
      421 cgcagcgcct ccctcacgag ccgcgagacc tccaccgccc ccgctccgtc gacgttcgcg
      481 cgccgctcca cgagtctcac cacgtgcgga tgcgtggggt tgacctcccc cgcctcctcg
      541 atcacgacga tccgctcgga cgggtcgacg agcgagagga ggctcgcgag gagcgtcgtc
      601 ttgccgctgc ccgtggcacc ggtgatgagg agcgaggcgc gtccggccac gagcccggcg
      661 agcacggggg cgagcccggg cgcgagcgtg ccgcagagtt cgaggtcgtc gagcgtcagc
      721 gccccgcgac ggaccgtcct gagggagatc gccgcgcacc cgtcgacgac gggcggaagg
      781 gccgcgtgga gtcgcgtgcc gtcggggagg cgggcgtcga cgagcggaca ggcatcgtcg
      841 agcctccgac cgccgaggga ggcgagtcga accgcgagtt cgcgcacgtc gcccgcggag
      901 gcgaagccga cggacgcgcg ctcaaggccc atgccgcggt cgatccacac ctcgtccggc
      961 ccgttgacaa ggacgtccgt gacgccgggc gtctcaagaa ggctacggag cggcccggcg
     1021 acgaagggaa gaagcgcgct catgtgcgcc acgatccccg cgccacccgc gcgccgccca
     1081 tcgctcaccg gcaacgcgcg ttcccgcccc ggtccccgcg acctggggac accggaatct
     1141 cgcgcccgtc cggccggtcc tccttcgggg gctccccggg ctcgtagact ggaacgtggg
     1201 agggaccatg acggagcacg gctccacggc agcattcttc gacctcgaca agacgatcat
     1261 cgcgacgtca tcgacggcta ggttctcgcg cgacctacgc tccaacgggc ttctcaagac
     1321 gagcgacgcg ctcaggaccg cgtacgccca attcctcttc atggtcggcg gcgccgacgc
     1381 ccgtcagacc gcccggctcc gcgacgcgct cgccgagacc atcaaggggt gggaggcggc
     1441 gaaggtccgc cggatcgtcg gcgagacggt caagcaactg atcgacccgg tcgtctacga
     1501 ggaggctctc accctcatcc ggcgccacca agccaacggc cgcgacgtgg tcatcgtgag
     1561 cgcatccggc gccgaggtcg tccagccgat cgcgagcctg ttgggcacca aggatttcat
     1621 cgcctcgcgt atggaggtgg tcgacggcgt cttcaccggg aagatcagcc ggtacgtctt
     1681 cgggccctac aaggccgacg cgattcgcgc catggcggcc cagcggggat acgagctcga
     1741 acgctgctac gcctactccg actccatcac cgacgcgccc atgcttgacg ccgtcggcta
     1801 cgggttcgcc gtcaatccca accccgcgat gcgacgcgcc gccgccgccc acggctgggg
     1861 cgtgctccac ttccggcgcc cggtagccct gcgcgaaagc aggcgccgca ggggaatcgt
     1921 cgtgggcgcg acggtcgcgg gcgtggcgct gacgacggcg ctcatctggt ggggcgtcac
     1981 ccgccgccgc gaccgcgaga tcgtctagtt cacctgcggc tgccccctcg caaccgatcc
     2041 tcgcccccaa ccgaaccgcg ctctcgcccg ttcaagttcc aggaaccgag cacggacatc
     2101 ggaggcggca tgagcgggtg gcgggggctg gcggccgagg tcgttgacgc gcgctacccg
     2161 tccctcgtcg cgtagggagt tctgctcaca gcgagccgcg ccgacgccga ggacctggtc
     2221 caggacgccc tcgtcaaggt cttctcgcgc ctacgccccc tgcccaacgt cgcggcggcc
     2281 gaccagtacg tccgccgcgc catgctgacg ctctacctcg accgcacgcg ccgcgtcacc
     2341 atgtggcgcc gcctccgcgg gaccttggcg acggacgacg ccgtggccgg gccggaggac
     2401 gcggcggccg cgggcacgga cgtccgcgcg gcgctcgccg ggctcagccc ccgcgagcgc
     2461 acctgcgtgg tgctgcgtca cttcgacgac ctctcgacgc gccaggtcgc cgaggcgacg
     2521 ggcctcgcgg agggaacggt caagcgctac ctcagcgacg cgctcgccaa gcatgaaggt
     2581 gcaggtgagc ggcccgagcg acatctcgag ctgcaccgcc tctccgggct gcagcggcgc
     2641 cggcagggag tacgaggaca tgttcacctg cgcgttgccg a
//
LOCUS       DASVKP010000034         4613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1326429, whole genome shotgun sequence.
ACCESSION   DASVKP010000034 DASVKP010000000
VERSION     DASVKP010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326429"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VF362_00685"
     CDS             complement(<1..967)
                     /locus_tag="VF362_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX7589479.1"
                     /translation="MSVQAAPNRDPWVIRTEAIADPGDLIAALPVPGISWVRRGEGMV
                     AWGEVARFEVRGASRFAEARAWWADACSHAIVRDDVGVRGSGLVAFGSFSFADASPAG
                     GVLIVPRYVLGRRNGLSWLTVISDSATISPPGLDEACAEATPPRPLGRVDVDAGDQAT
                     WAAAVGAAIARINAGAVDKVVLARAVDARADGPVDVRSLLAPLARDYPTCWTFHVDGM
                     FGATPELLARVDRGLVTSRVLAGTIRPTGDETADLAHAAALARSSKDREEHEYAVRSV
                     ADVLERHCSSINVPEAPSVIHLPNVMHLSTDVTGVLSDHTTSLELV"
     gene            1057..2340
                     /locus_tag="VF362_00690"
     CDS             1057..2340
                     /locus_tag="VF362_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEX7589480.1"
                     /translation="MKTDADAIIVGAGPAGSAVAHYLAKEGFSVIALEKARFPRDKVC
                     GDGLTPRAVRELSLMGVPTPESEGWVRNRGLRIIGGGHRLEFPWIESASFPGYGLTLP
                     RAQFDHVLARHARATGADVREGVAVEGLVHDERTGRAVGVTARQTDEKGRKVGDVVTY
                     RASLVIAADGASSRIATSEGIERKKGRPMGVAARAYFRVDRPRDEWMEGHLELWSGEA
                     GKSKLLPGYGWIFTVGDGLVNVGLGTLSGRGKPVGLDHRAMLEEFVKNTAGEWGFTED
                     DRAGETLGAAIPMAFSRQPHYRDGLMLVGDSGGMVNPFNGEGIAYAMQAARRATEAAV
                     GAREATGERDRERALSGYARQMKMDLGGYYSMGRVFAALIERPSVMRACTKYGLPRPT
                     IMWITHKLLSDVYEPHGGDWADRILATMARVAPKA"
     gene            2396..2731
                     /gene="ndhC"
                     /locus_tag="VF362_00695"
     CDS             2396..2731
                     /gene="ndhC"
                     /locus_tag="VF362_00695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX7589481.1"
                     /translation="MMIAVAGVLAVAGVGLSAVLGPKRYNQAKLDRYESGVQPTPAAE
                     GGGRVPIKYFLVAMTFIIFDVEVVFLYPWAVAYQQFALFGYIAIVTFLALITVPFIYE
                     WRRGGFDWE"
     gene            2735..3286
                     /locus_tag="VF362_00700"
     CDS             2735..3286
                     /locus_tag="VF362_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX7589482.1"
                     /translation="MGLEESAPGFLLGTVEGVAGWARGASVYPVTFGLACCSIEMMSA
                     GGPRFDIARMGSEVFRGSPRQADLMIVAGRVSQKMAPILRQVYDQMAGPKWVIAMGVC
                     ASSGGMFNNYAILQGVDHVVPVDVYLPGCPPRPEMLLNALMMIKKHARETPMGANRRR
                     IALAAEQAALEAPPTSDKTGLLR"
     gene            3298..3975
                     /locus_tag="VF362_00705"
     CDS             3298..3975
                     /locus_tag="VF362_00705"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX7589483.1"
                     /translation="MAGPPERDGRTPMTLLSVRDGMFSGSDDTSGYGGLEQTFAMPGP
                     TERPYGEWFDGSVDILRELLGANADAAIEAVVVDRGELTLLVARAFIVEVCQHLRDDR
                     DLRFELSLGVSGVHYPADAGRELHAVYHLRSITHNRRLRLEVAVADADPHVPSTTAVY
                     PANNWHERETYDFFGIIFDGHPSLARIEMPDDWVGHPQRKDYPLGGVPVEYKGATIPS
                     PEDRRQY"
     gene            3975..>4613
                     /locus_tag="VF362_00710"
     CDS             3975..>4613
                     /locus_tag="VF362_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit D"
                     /protein_id="HEX7589484.1"
                     /translation="MATRATGAIHEDASVPEFTASGGDWSDVTEEAARLSGQRIVVNM
                     GPQHPSTHGVLRIMLEIDGETITEARAGVGYLHTGIEKSMEWRTWTQGSTLCTRMDYL
                     ASMFQEAAFCLATEKLLGITDDIPERASVLRVLMMELNRISSHLVAIGTGGNELGATT
                     IMTTSFLAREDVLRIIEAVTGLRMNNAYIRPGGVATDAPEGLVDMLVELDGKL"
BASE COUNT          721 a         1576 c         1622 g          694 t
ORIGIN      
        1 ccaccagttc gaggctcgtc gtgtggtcgc tgagcacgcc cgtcacatcg gtcgagaggt
       61 gcatcacgtt gggcaggtgg atgacggagg gcgcctcggg aacgttgatc gagctgcaat
      121 ggcgctccaa gacgtctgcc accgacctga ccgcgtactc gtgttcctct cggtccttcg
      181 aggagcgcgc gagcgccgcg gcatgcgcga ggtcggcggt ctcgtcgccg gtcgggcgaa
      241 tcgtcccggc gagcacgcgc gaggtgacga gtccgcggtc gacccgggcg aggagctcgg
      301 gcgtcgcgcc aaacatgccg tcgacgtgga aggtccagca ggtggggtag tcccgcgcga
      361 ggggagcgag gagcgatcgg acgtcgacgg ggccgtcggc gcgcgcgtcc accgcgcgag
      421 cgaggacgac cttgtcgacc gcgccggcgt tgatgcgcgc gatggcggcc ccgaccgccg
      481 ccgcccaggt tgcctggtcg ccggcatcga catcgacgcg accgagcggg cgcgggggcg
      541 tcgcctccgc gcacgcctcg tcgagaccag ggggggagat cgtcgcggag tcggagatga
      601 cggtgagcca ggagagcccg ttgcggcgcc cgaggacgta tcgaggaacg atgagcactc
      661 ctccggcggg cgacgcatcg gcgaaggaga acgagccgaa ggcgacgagg ccggagcccc
      721 ggacgccgac gtcgtccctc acgatggcgt gggagcacgc gtcggcccac caggcgcgcg
      781 cctcggcgaa acgggacgcg ccgcgaacct cgaaccgggc gacctcgccc catgccacca
      841 ttccctcgcc ccgtcgaacc caggagattc cgggtacagg caaggcggcg atcaagtccc
      901 cggggtcggc aatcgcctcg gtcctgatca cccacgggtc gcggttcggg gccgcctgca
      961 cgctcacgcc cccagcctaa gtccgcgtgt cgcgcgcgcc ccccacggtc ccccggcggc
     1021 gcgaggccgc ccccgaggag aacgtaggct gagggggtga agacggacgc cgacgccatc
     1081 atcgtgggcg cgggcccggc cggatccgcc gtcgcccact acctcgccaa agagggcttc
     1141 agcgtgatcg cgctcgagaa ggcgcgcttc ccgcgcgaca aggtgtgtgg ggacgggctg
     1201 acgccccgcg ccgttcgcga actctccctg atgggcgtcc ccactcccga gtcggagggc
     1261 tgggtacgca acagggggct gcgcatcatc ggcggcgggc accgactcga gttcccgtgg
     1321 atcgagagcg cctccttccc cgggtatggc ttgacgcttc cgcgcgccca gtttgaccac
     1381 gtgctcgcca ggcacgcgcg ggcgacgggc gcggacgtgc gcgagggcgt cgcggtcgag
     1441 ggcctcgttc acgacgagcg cacgggccgg gcggtcgggg tgacggccag gcagacggac
     1501 gagaagggtc gcaaggtcgg cgatgtcgtc acgtacaggg cctcccttgt gatcgcggcc
     1561 gatggggcca gctcgcgcat cgcgacgagc gaggggatcg agcgcaagaa gggccgaccc
     1621 atgggggtcg ccgcccgcgc ctacttccgc gtcgaccgcc ccagggacga gtggatggag
     1681 ggccacctcg aactctggtc gggcgaggcg ggcaagtcga agctcctccc cggctacgga
     1741 tggatcttca ccgtcggcga cgggctggtc aacgtcggcc tcgggacact gagcgggcgc
     1801 ggcaagccag tcggcctcga tcacagggcg atgctcgagg agttcgtcaa gaacaccgcg
     1861 ggcgagtggg gattcacgga ggacgacagg gcgggcgaga cgctcggcgc cgccattccc
     1921 atggccttca gccgccagcc gcactaccgc gacggcctca tgcttgtggg cgacagcggg
     1981 gggatggtca acccgttcaa cggcgagggc atcgcctacg cgatgcaggc ggcgcgccgc
     2041 gcgaccgagg ccgccgttgg cgcgcgcgag gcgacgggcg agcgggaccg cgagcgcgcg
     2101 ctctccgggt acgcccgcca gatgaagatg gacctgggcg gctactacag catggggcgc
     2161 gtcttcgccg ccctcatcga gcgtccgtcc gtcatgaggg cgtgcaccaa gtacggactt
     2221 ccgcgtccta ccatcatgtg gattacccat aaactgctgt ctgacgtcta cgagccgcat
     2281 ggtggcgact gggccgatcg aatcctcgcc acgatggcgc gtgtcgcgcc gaaggcgtga
     2341 ttgtgggccg agtcaggaag ggagcctttc ggtgaacccc tacgtgccgg tgatcatgat
     2401 gatcgccgtg gctggcgtgc tcgcggtagc tggcgtcggg ctcagtgccg tccttgggcc
     2461 caagcgctac aaccaggcga aactcgaccg ctacgagtcc ggggtgcagc cgacgcccgc
     2521 ggccgaaggc gggggcaggg ttccgatcaa gtacttcctc gtggcgatga ccttcatcat
     2581 cttcgacgtc gaggtggtgt tcctctaccc ttgggcggtg gcgtaccaac agttcgcgct
     2641 gttcggctac atcgccatcg tgacgttcct tgccctcatt accgtgccct tcatctatga
     2701 gtggcgccgc ggcggattcg actgggagta atcgatgggt cttgaggaga gcgcgccggg
     2761 gttccttctc ggtacggtcg agggcgttgc gggttgggcc cgcggcgctt cggtgtaccc
     2821 ggtgaccttc ggcctcgcct gttgctcgat cgagatgatg tcggccgggg ggccgcgctt
     2881 cgacatcgcg cggatgggtt ccgaggtctt ccgcggctcg ccgcgccagg cggacctcat
     2941 gatcgtcgcc ggccgcgtca gccagaagat ggcccccatc ctgcgccagg tctacgacca
     3001 gatggccggg cccaagtggg tcatcgcgat gggtgtctgc gcatcgtccg gggggatgtt
     3061 caacaactac gccatcctcc agggcgtcga tcacgtcgtt cccgtcgacg tctacctccc
     3121 cggctgtccg ccgcgccccg agatgctcct caatgcgctg atgatgatca agaagcacgc
     3181 ccgcgaaacc cccatgggcg ccaaccggcg ccgcatcgcc ctggcggcgg agcaagccgc
     3241 gctcgaggct ccgcccacgt ccgacaagac gggacttctc cggtgagcgg cgacgaggtg
     3301 gctggcccgc ccgagcgcga tgggcggacg cccatgactc ttctatccgt gcgagacggc
     3361 atgttctcgg gctccgacga cacctccggc tacggtgggc tcgagcagac gttcgccatg
     3421 cccggcccga cggagcgccc ctacggcgag tggtttgacg gatccgtcga catcctcagg
     3481 gaactgctcg gcgcgaacgc ggacgccgcg atcgaggccg tcgtggtgga tcgcggcgaa
     3541 ctgaccctcc tcgtcgcgcg ggccttcatc gtcgaggtct gccagcacct tcgcgacgat
     3601 cgggatctcc gcttcgagtt gagcctcggc gtgagcgggg ttcactaccc cgcggacgct
     3661 gggcgcgagc ttcacgccgt ctatcacctg cggtcgatca cgcacaaccg acgccttcgc
     3721 ctcgaggtcg cggtggccga cgccgacccc cacgtcccga gcacgaccgc ggtctacccc
     3781 gccaacaatt ggcacgagcg cgagacgtac gacttcttcg gcatcatctt cgacgggcac
     3841 ccctcgctcg cccggatcga gatgcccgac gactgggtgg gtcatcccca gcgcaaggac
     3901 tatccgctcg gcggcgtgcc cgtcgagtac aagggcgcga ccatcccgtc gccggaagac
     3961 cggaggcagt actaatggcc acgcgcgcca ccggcgcgat tcacgaggat gcgtcggttc
     4021 cggagttcac cgcctcgggc ggcgactggt ccgacgtcac ggaggaggcc gcccggctct
     4081 cgggccagcg gatcgtggtg aacatgggcc cgcagcaccc ctcgacccac ggcgtcctgc
     4141 gcatcatgct cgagatcgac ggcgagacca tcaccgaggc ccgcgcgggc gtcgggtacc
     4201 tgcacacggg tatcgagaag agcatggagt ggaggacctg gacccagggc tccaccctct
     4261 gcacccgcat ggactacctc gcgtccatgt tccaggaggc cgccttctgc ctcgcgacgg
     4321 agaagttgct cggcatcacc gacgacatcc ccgagcgcgc gagcgtgctg cgcgtgctca
     4381 tgatggaact caaccgcatc tcgtcacacc tggtcgcgat cgggaccggc gggaatgagc
     4441 tcggcgccac cacgatcatg acgacctcct tcctcgcccg cgaggacgtt ctccgcatca
     4501 tcgaggccgt gacggggctt cggatgaaca acgcctacat ccggccggga ggcgtcgcga
     4561 cggacgcgcc ggagggtctc gttgacatgc tcgtcgagct tgacggcaaa ctc
//
LOCUS       DASVKP010000035         1726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_504438, whole genome shotgun sequence.
ACCESSION   DASVKP010000035 DASVKP010000000
VERSION     DASVKP010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504438"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VF362_00715"
     CDS             <1..1064
                     /locus_tag="VF362_00715"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEX7589485.1"
                     /translation="FLGLPPFLADSLPDDFGNAIVDAYLAREGIDAGEFTALDRLVYA
                     GGRALGALEFHPPAPRVQTPSALDLGTLVTEARRALRGDLAHDPETSIRDLFGVGTSA
                     GGARAKAVIAIDAAGRVRSGSVPPERGWTDYLLKFDGTGNDAHATDGYTRIEYAYHLM
                     AAAAGITVPEARLLEEGGRAHFLSKRFEREDGTRVHLQSLCAMRGLDFRLVGTHDYGQ
                     YLATIGELGLGADAVAQGFRRAAFNVFAFNRDDHTKNVAFLMDPDGRWRLAPAFDLTF
                     SFRPDSPWVARHQMGVDGVFADPTRRDLERLGDRWAVPGIRGILGEVADAVARWRDFA
                     DEAGVEAGRRDRIGALILR"
     gene            complement(1083..1253)
                     /locus_tag="VF362_00720"
     CDS             complement(1083..1253)
                     /locus_tag="VF362_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7589486.1"
                     /translation="MHVPPHADIDEIALAMADYNLLVLPVVDQDYRLIGVLTSDDVLE
                     AAIAPERRRRRG"
     gene            1236..>1726
                     /locus_tag="VF362_00725"
     CDS             1236..>1726
                     /locus_tag="VF362_00725"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589487.1"
                     /translation="MRGDVHGGVLGELDEGRARVGLDEGDQASDLLEARTDPQIADVL
                     GRMRADDAADALLDLPQERRMGVLRQLPDPQRKKISALLGYHESTAGGLMSLDHLLLP
                     AATTAREAIAAVRAATQMQPEAIVVVFCHDADHALSGAVSLVDLVQADPGATLVELAE
                     DAPV"
BASE COUNT          236 a          612 c          632 g          246 t
ORIGIN      
        1 ccttcctcgg gttgcccccg ttcctcgcgg actcgctccc cgacgacttc gggaacgcga
       61 tcgtcgacgc ctacctcgcg agggagggga tcgacgcggg ggagtttacc gcgctcgacc
      121 ggctcgtgta cgcggggggc agggcgctcg gcgcgctcga gttccacccg cccgcgcccc
      181 gggtccagac gccgagtgcc ctcgatctgg gcaccctcgt cacggaggcg cgccgggccc
      241 tccgcggcga cctcgcgcac gaccccgaga cctcgattcg cgacctcttc ggcgtcggga
      301 cgtccgcggg gggcgcgagg gccaaggccg tgattgcgat cgacgccgcg gggcgcgtgc
      361 gctctggttc cgttccgcca gagcggggct ggacggacta tctgctcaag ttcgacggga
      421 cgggcaacga cgcgcacgcg acggacggat acacgaggat cgagtacgcg taccacctca
      481 tggcggcagc cgcggggatt acggtgcccg aggcgaggct cctggaggag ggcgggcgcg
      541 cgcacttcct gagcaagcga ttcgaacgcg aggacggcac gcgcgtgcac ctccagtccc
      601 tgtgcgccat gcggggcctg gacttccggc tggtcggcac ccacgactac gggcagtacc
      661 tcgcgacgat cggggaactc ggcctcggcg ccgacgcggt cgcccagggg ttccgtcgcg
      721 cggccttcaa cgtgttcgcg ttcaacaggg acgaccacac gaagaacgtg gccttcctca
      781 tggaccccga cggtcggtgg cgcctcgcgc ccgcgttcga cctcacgttc tcgttccgcc
      841 cggattcgcc gtgggtcgcg cggcaccaga tgggcgtcga cggcgtgttc gcggacccga
      901 cgcgacggga cctcgagcgg ctcggcgacc gatgggcggt cccggggata cgggggatcc
      961 tcggcgaggt cgcggacgcg gtggcgcggt ggcgcgactt cgcggacgag gcgggcgtcg
     1021 aggccgggcg ccgggacagg atcggcgcgc tcatccttcg gtagtgcgct cttggttggc
     1081 cgctatccgc gccggcgtcg ccgttcgggc gcgatggccg cctcgaggac gtcgtcgctc
     1141 gtgaggactc cgatgaggcg gtagtcctgg tcgacgacgg gcaggaccag caggttgtag
     1201 tcggccatgg ccagggcgat ctcgtcgatg tcggcgtgcg gggggacgtg cacgggggcg
     1261 tcctcggcga gctcgacgag ggtcgcgcca gggtcggcct ggacgagggc gaccaggcct
     1321 cggacctgct cgaagcgcgc accgatcccc agatcgccga cgtcctgggt cgcatgcgcg
     1381 ccgatgacgc cgccgacgcg ctgctggacc tgccgcagga acgacgcatg ggcgtcctgc
     1441 gacagttgcc cgacccccag cggaagaaga tcagcgcact tctcggctat cacgaatcca
     1501 cggccggcgg gctgatgagc ctggaccacc tcctcctccc cgccgccact acggcaaggg
     1561 aggccatcgc cgccgtccgg gcggcgaccc agatgcagcc cgaagccatc gtcgtcgtgt
     1621 tctgccacga cgctgaccac gccctgagcg gcgcggtcag cctggtcgac ctcgtccagg
     1681 ccgaccctgg cgcgaccctc gtcgagctcg ccgaggacgc ccccgt
//
LOCUS       DASVKP010000036         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_833412, whole genome shotgun sequence.
ACCESSION   DASVKP010000036 DASVKP010000000
VERSION     DASVKP010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833412"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1882)
                     /locus_tag="VF362_00730"
     CDS             complement(<1..1882)
                     /locus_tag="VF362_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7589488.1"
                     /translation="MSTTTERAGGAPRGDDVRRYVDATRALQATSNEVHDLESSEGVE
                     SFNRQVEVLKRRLLEDPKAFRDMFIADGMAAAAWEFNQSDLSPEFVERLWTLLQRDDD
                     ASTVIMRFIWNMPIGKKRAFIRGLDKNLGQRYPMFDGLSTDWPAGSSIPPYIRTPEQR
                     SDDFGLVNQGYYGYMNLGFTARDVELLVWLEALRDKQCAESPCELGIFVAEHKPPKGG
                     CPVRIHIPQMLELIGQGRFREAFELIEGSNPLPDVTGRVCPQELQCQGVCLHKLPMAI
                     GQVEWFLPQHQKMVDPGAAERRFAGFPDPWKTATKPPIAIVGSGPSGLINAYLLAVEG
                     FPVTIFEAFHALGGVLRYGIPEFRLPNDLIDDVVGKIKLLGGRFVENFVVGKTGTLQD
                     LKDAGFYKIFVGSGAGLPRFMNVPGEHLLNVMSANEFLTRVNLMQGLRADYETPLPEI
                     RGKEVIVIGGGNTAMDAARTSRRLGGNVTIVYRRTKEEMPARVEELHHAIEEGITVME
                     LRGPTEFLGNDKNFVTAATVDIMTLGEPDESGRRKPVSTGETATIKADLAIMALGNDA
                     NPIIKDSEPRLKTSKWGTIELQREGSQETTLADVYTGGDAARGGSTAIMAAGDGQSAA
                     REI"
     gene            complement(1879..>2146)
                     /locus_tag="VF362_00735"
     CDS             complement(1879..>2146)
                     /locus_tag="VF362_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589489.1"
                     /translation="SAASGSEASAAGDKPVWLNPSDEHLCNDCGTCYQELPQFFRKKT
                     EVVDGEARILAEFIPGSTDGVEVTAELAKRIARVKDTCDAEIIR"
BASE COUNT          308 a          691 c          752 g          395 t
ORIGIN      
        1 ggatctcccg cgccgcgctc tggccgtctc cggccgccat gatcgccgtg gagcctccgc
       61 gcgcggcgtc gccgccggtg tagacgtccg ccagcgtcgt ttcctgcgaa ccctcgcgct
      121 ggagttcgat cgtgccccac ttggaggtct tgaggcgcgg ttcggagtcc ttgatgatcg
      181 ggttggcgtc gtttccgagc gccatgatcg ccaggtcagc cttgatggtc gccgtctcgc
      241 ccgtgctgac gggcttgcgg cgtcccgact cgtcgggctc gcccagggtc atgatgtcga
      301 ccgtcgcggc ggtgacgaag ttcttgtcgt ttccgaggaa ctccgtcggc ccgcggagtt
      361 ccatgacggt gatgccctct tcgatcgcgt ggtgcaactc ctccacgcgg gcgggcatct
      421 cctccttggt gcggcgatag acgatggtga cgtttccgcc gaggcggcgc gacgtgcgcg
      481 cggcgtccat agcggtgttg ccaccgccga tgacgatgac ctccttgccg cggatctcgg
      541 gaagcggcgt ctcgtagtcc gcgcgaaggc cctgcatcag gttgacgcgc gtgaggaact
      601 cgttggccga catgacgttg agcaggtgct cgccgggaac gttcatgaag cgggggagcc
      661 ccgctccgga gccgacgaag atcttgtaga agcccgcgtc cttgaggtcc tggagcgtcc
      721 ccgtcttgcc gacgacgaag ttctccacga accggccgcc cagcaacttg atcttgccca
      781 cgacgtcgtc gatcaggtcg ttggggaggc ggaactcggg gatgccgtac cggagcacgc
      841 cgccgagggc gtggaacgcc tcgaagatcg tgacggggaa gccctcgacc gcgaggaggt
      901 acgcgttgat gaggcccgac gggccggagc cgacgatcgc gatgggcggc ttggttgccg
      961 tcttccacgg gtcggggaag cccgcgaagc gacgctcggc ggcgccgggg tccaccatct
     1021 tctggtgctg cgggaggaac cactcgacct ggccgatcgc catggggagc ttgtggaggc
     1081 acacgccctg gcactggagt tcctgcgggc agacgcgccc cgtgacgtcg gggagcgggt
     1141 tcgagccctc gatgagttcg aaggcctcgc ggaagcggcc ctggccgatc agctccagca
     1201 tctgcgggat gtggatccgc acggggcacc cgccctttgg cggcttgtgc tcggcgacga
     1261 agataccgag ctcgcacggg ctctcggcgc actgcttgtc ccggagcgcc tcgagccaaa
     1321 cgaggagttc gacgtcgcgc gcggtgaatc caaggttcat gtagccgtag tagccctggt
     1381 tcaccaggcc gaagtcgtcc gagcgctgct cgggggtgcg gatgtagggc ggaatcgaac
     1441 tcccggcggg ccaatcggtg ctcagcccgt cgaacatcgg gtagcgctgg cccaggttct
     1501 tgtcgaggcc gcggatgaac gcgcgcttct tgccgatggg catgttccag atgaaccgca
     1561 tgatgacggt gctggcgtcg tcgtcgcgct ggaggagggt ccagagccgc tcgacgaact
     1621 cgggggagag gtcactctgg ttgaactccc acgcggcggc cgccatcccg tccgcgatga
     1681 acatgtcgcg gaaggccttc gggtcttcga ggaggcgacg cttcagaacc tccacctgcc
     1741 ggttgaagga ctcgacgccc tcgctggatt cgaggtcgtg gacctcattg ctcgtcgctt
     1801 ggagcgcgcg ggtcgcgtcg acgtatcgac ggacgtcatc gccccgtggc gcgccgcccg
     1861 ctcgctccgt cgtcgtgctc atcgaatgat ctccgcgtcg caggtgtcct tcacgcgtgc
     1921 gatgcgcttg gcgagttcag ccgtgacttc cacgccgtcg gtcgagccgg gtatgaactc
     1981 ggcgaggatc ctcgcctctc cgtccacgac ctcggtcttc ttgcggaaga actgcggcag
     2041 ctcctggtag caggtgccgc agtcgttgca gaggtgctcg tcgctcggat tgagccacac
     2101 gggcttgtcg cccgcggcgg acgcttccga gccggacgcc gcagac
//
LOCUS       DASVKP010000037         7068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1572731, whole genome shotgun sequence.
ACCESSION   DASVKP010000037 DASVKP010000000
VERSION     DASVKP010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572731"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VF362_00740"
     CDS             <1..186
                     /locus_tag="VF362_00740"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879833.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX7589490.1"
                     /translation="ETEQVRLSIGADSLGHLSLEGMIAATKQPAHNLCTACFTGQYPI
                     EPPADALHLLAHQDGES"
     gene            183..1307
                     /gene="purM"
                     /locus_tag="VF362_00745"
     CDS             183..1307
                     /gene="purM"
                     /locus_tag="VF362_00745"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX7589491.1"
                     /translation="MSARIPGTDAVTYAGSGVDTAAGDRAVELMKAAVARTHGPEVVG
                     GVGGFAGLFDASALKNYRRPLLASSTDGVGTKIAIARALGIHDTIGRDLVAMVVDDIV
                     VCGATPLVMTDYIACGKVVPERIADIVRGIAEGCVDAGVSLVGGETAEHPGLMAEDDY
                     DVAGAAIGVVEADAVLGPDRVRAGDVLVGLASSGLHSNGYSLVRAVVARAGWTLDRLV
                     PEFGRTLGEELLEPTRIYSRLCVELAANIPALHALAHVTGGGLAANVARVIPLGLAAS
                     VARNAWAVPQVFEVLRAAGDVPWRDLEATLNLGVGMVAIVAPEGASALMRACEDSGVA
                     AWGLGEVVGDDGRAVSEDVLRGAKGVDGGAVYLGGSYRTA"
     gene            complement(1346..1543)
                     /locus_tag="VF362_00750"
     CDS             complement(1346..1543)
                     /locus_tag="VF362_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 domain-containing protein"
                     /protein_id="HEX7589492.1"
                     /translation="MGRGRQKAKDAKVARQLKYSGPGSDLKALERELIVGGSTVAAVV
                     SHPTDDDTDDEYADRWDDETE"
     gene            1761..1964
                     /locus_tag="VF362_00755"
     CDS             1761..1964
                     /locus_tag="VF362_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HEX7589493.1"
                     /translation="MTEKLTEPEKLLTPSEVAAIFRVDPKTVTRWAKVGKLSSIRTLG
                     GHRRFREAEVKMLLAAAQETRFA"
     gene            2032..2556
                     /locus_tag="VF362_00760"
     CDS             2032..2556
                     /locus_tag="VF362_00760"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX7589494.1"
                     /translation="MSEERPLVGSEFATPFGPVAVLVSPEDGVLRASSLSSLSHLVGT
                     LPPALARRGVEPGENPGIACAIAAWLSGDGSALAAVPARQAGGSFMQRVWEAMRKIPP
                     GQVMTYGELAAAAGNPRAARAAGHACSTNTLAPFVPCHRVVASTGLGDYGFGVEIKAR
                     MLRLEGARIGRTAV"
     gene            complement(2627..3013)
                     /locus_tag="VF362_00765"
     CDS             complement(2627..3013)
                     /locus_tag="VF362_00765"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX7589495.1"
                     /translation="MARSFFSSFSVSSIVSAILSQVGGVVRRELEIAKREFVGKVKAL
                     FAGGVLVAIGMSLSMSALILFIIAGVSALTLIWPLWLSALVGGGSLLVIALIFLAIGA
                     SKIKKNMDLRPERAISALKRFSHEID"
     gene            complement(3111..3183)
                     /locus_tag="VF362_00770"
     tRNA            complement(3111..3183)
                     /locus_tag="VF362_00770"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3148..3150),aa:Phe,seq:gaa)
     gene            complement(3223..3296)
                     /locus_tag="VF362_00775"
     tRNA            complement(3223..3296)
                     /locus_tag="VF362_00775"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3260..3262),aa:Asp,seq:gtc)
     gene            complement(3436..3508)
                     /locus_tag="VF362_00780"
     tRNA            complement(3436..3508)
                     /locus_tag="VF362_00780"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3472..3474),aa:Glu,seq:ttc)
     gene            3607..3960
                     /locus_tag="VF362_00785"
     CDS             3607..3960
                     /locus_tag="VF362_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX7589496.1"
                     /translation="MVQMTRAEFEEAVVDALDQIPEELARLMDDVVVLVEDDSPAGGP
                     VLLGLYEGVALTDRGNSWGHGELPDRIFIYRNPTLAMCRTAKEVRDEVAVTVVHEIAH
                     HFGIDDDRLHELGWG"
     gene            4018..4395
                     /locus_tag="VF362_00790"
     CDS             4018..4395
                     /locus_tag="VF362_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEX7589497.1"
                     /translation="MRTIFPSGDDYEDYYGYSRTVRVGNQVSVSGTTARAPHDHSTDA
                     HAQAVSALDVIRGALEEAGATMADVTRTVAYITSIDYVDAVARAHKEAFNDIRPAATC
                     VVVTSLVVPHLLVEIQVDAVVPS"
     gene            complement(4418..5392)
                     /gene="galE"
                     /locus_tag="VF362_00795"
     CDS             complement(4418..5392)
                     /gene="galE"
                     /locus_tag="VF362_00795"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX7589498.1"
                     /translation="MSWLVTGGAGYIGAHVVRALLEADFGVAVLDDLSTGREEFLPHG
                     VALTRASVLDTSAVEAALREHAIDGVIHCAGFKFAGESVRRPLHTWRQNLSGTESLLR
                     AMESVGVARIVFSGSAAVYGTPREELVTEETPTAPESPYGESKLAAELLIRGQVLATA
                     EMEAPLAGTSLRYFNVAGSADPAVFDVSPYNVLPRVFTGLVEGRAPVINGDDFDTPDG
                     TCVRDYIDVGDVALAHAAAARAFAAGRALEPVYNLGSGTGTSVRELIDAVLKVSGTSL
                     VPTAGPRRAGDPARIVADGTLAARDLGWAMRHPVRDMVASAWKAHPKG"
     gene            complement(5413..6585)
                     /gene="galK"
                     /locus_tag="VF362_00800"
     CDS             complement(5413..6585)
                     /gene="galK"
                     /locus_tag="VF362_00800"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEX7589499.1"
                     /translation="MTAPEPCGAAASGAFAATFGHVPAVLASAPGRVNLIGEHTDYND
                     GLVLPFAIERRTWCALAPRSDGILRVTTTLNDAVVELGVDDLDPENLAGWSAYVFGVV
                     WALREHGVDVASRGGLDIAVASDVPLGAGLSSSAALECAVAVAIDAEWELGLGVDVLA
                     RVCQRAENGAVGAPTGIMDQTASLLGQAGHGVLLDCRDNHSEIVPLCFAENGLDVMVV
                     DTRVTHEHATGGYAERRASCDRGAAALGVSSLRDLSVADMVRAQAVLDDETFRRVRHV
                     VTENDRVLAAATTLENDGPRAIGPLLLASHASMRDDFEISVPELDRAVEVAMEAGAIG
                     ARMTGGGFGGSAIALVPREKFELAEATVLSAFEASGFREAHIFTVVPSDGARRETL"
     gene            complement(6582..>7068)
                     /locus_tag="VF362_00805"
     CDS             complement(6582..>7068)
                     /locus_tag="VF362_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEX7589500.1"
                     /translation="VRAEVARYGPTLFADILDRERLSTRMLHAGVHWSAFVPFAARWP
                     IEIHVLPHRDIRDLAQTSLAERDELALLLPRLLGAVDAMYDGPTPYIASWHQAPLVGG
                     DDIRLMFKIESPRRAADKLKFLAGSEAAMGAWVADVSPESAAGRLREALATVDQKAGA
                     R"
BASE COUNT         1128 a         2439 c         2455 g         1046 t
ORIGIN      
        1 gagaccgagc aggtgcgcct cagcatcggg gccgattccc tcggccacct ctcgctcgag
       61 gggatgatcg ccgcaacgaa gcagcccgcg cacaacctgt gcaccgcctg cttcacgggg
      121 cagtacccca tcgagccccc ggcggacgcc ctccacctcc tcgcgcatca ggacggggag
      181 tcgtgagcgc gcgcattccg gggactgacg cagtcaccta cgccggatcg ggcgtcgaca
      241 ccgcggccgg ggaccgggcc gtcgagttga tgaaggccgc ggtcgcgcgc acccatggcc
      301 ccgaggtcgt cggcggcgtc ggcggattcg cgggactctt cgacgcgagc gcactcaaga
      361 actaccggcg acccctcctc gcgtcctcca cggacggggt gggcaccaag atcgccatcg
      421 cccgagcgct cggcatccac gacaccatcg ggcgcgacct ggtcgccatg gtcgtggatg
      481 acatcgtcgt gtgcggggcc acccccctcg tcatgacgga ctacatcgcg tgcgggaagg
      541 tcgttcccga gcggatcgcg gacatcgtcc gcggcatcgc cgagggttgc gtcgacgccg
      601 gcgtctctct cgtcggcggc gagaccgccg agcaccccgg cctgatggcc gaggacgact
      661 acgacgtggc cggggccgcg atcggcgtgg tcgaggccga cgcggtgctc gggccggatc
      721 gcgtgcgggc gggcgacgtc ctggtcggcc tcgcgtcgtc gggccttcac tccaacggct
      781 acagcctggt gcgcgcggtc gtcgcgcgcg cgggatggac gctcgaccgc ctcgtcccgg
      841 agttcgggag aaccctcggc gaggaactcc tggagccgac ccggatctac tcgcgcctgt
      901 gcgtcgagct cgcagcgaac attcccgcgc tccacgccct cgcgcatgtc acgggcggcg
      961 ggctcgcggc gaacgtcgcg cgcgtgattc cgctggggct tgccgcgagc gtcgcgcgga
     1021 acgcgtgggc cgtgccgcag gtgttcgagg tgttgcgcgc cgcgggcgat gtcccatggc
     1081 gcgaccttga ggcgacgctc aacctggggg ttggcatggt cgcgattgtg gcacccgagg
     1141 gcgccagcgc cctcatgcgc gcctgcgagg attccggggt cgcggcgtgg gggctaggcg
     1201 aggttgtcgg tgacgacggt cgcgcggtct cggaagatgt cctgcgcggc gccaagggcg
     1261 tcgacggcgg ggcggtctat ctgggtggtt cctaccgcac cgcttaaatc gacgcgcgta
     1321 gccaagggaa cccgcgcggg gtccgctact cggtctcgtc gtcccagcgg tcggcgtatt
     1381 cgtcgtccgt gtcgtcgtcc gtggggtgcg aaacgaccgc ggcaacggtg cttcccccga
     1441 cgatgagttc gcgctcaagt gccttgaggt cggagcccgg cccggagtat ttcagttgac
     1501 gcgcaacctt tgcgtccttg gctttctgac ggccgcgacc catctcgccg tcccctctcg
     1561 gaatcgaggg ccgctgacgc gggcccgtct caatagtcca cgagcaaact ctaccgcgag
     1621 tgccatggcg cgagcgaatg ggcagattgg acggaaaatc ggaaaagttg gcaaaaccgc
     1681 gtcatagtgg ctcttgcgac gtgtctcttc tcgtaggagc gaacacaacc gcgctccccg
     1741 gctcgaggga taccaacgtg atgactgaaa agttgacgga gccagagaag ctcctcacgc
     1801 cgagtgaggt cgccgcgatc ttccgcgtcg atcccaagac ggtgacccga tgggcgaagg
     1861 tcggcaagct ctcgtccatc cgcacgctcg gcgggcatcg ccgcttccgc gaagcggaag
     1921 tcaagatgct cctcgccgcg gcccaggaaa cccgcttcgc gtagccgttc gccgcgccca
     1981 ggccacgagt gccccgggcg cggtatcggc tcgtcgtcgt accctgggcc catgagcgaa
     2041 gaacgcccgc ttgttggctc ggaattcgcg acgcccttcg ggcccgtcgc cgtgctcgtt
     2101 tcgccggagg atggcgtcct gcgcgcctcg tccctgtcct cgctctctca ccttgtcggc
     2161 acgctaccgc ccgcgctcgc gcgccggggc gtcgagcccg gcgagaaccc cggcatcgcg
     2221 tgcgccatag cggcttggct gtccggcgac ggaagcgcgc ttgcggcggt tcccgcgcgg
     2281 caggccgggg gatcgttcat gcaacgcgtg tgggaggcca tgaggaagat ccccccgggc
     2341 caggtgatga cctacgggga actcgcggcc gccgcgggca atccccgggc cgcccgcgcc
     2401 gcggggcacg catgctccac caacaccctc gcgccgttcg ttccgtgcca cagggtggtc
     2461 gcgtcgacgg gactgggaga ctacgggttc ggcgtggaga tcaaggcgcg catgttgcgc
     2521 ctcgagggag ctcgaatcgg gcggaccgcc gtctagggga cggccgactc gggtatcgcc
     2581 gcgtcaagga ctctcgcccg aaggactcca gacaaaagaa gaccgcctag tcgatctcgt
     2641 gcgagaaccg cttcagggcg ctgatcgcgc gctcgggccg caggtccatg ttcttcttga
     2701 tcttcgaggc gccgatggcg aggaagatga gcgcgatcac caggagcgat cccccgccca
     2761 ccagggccga gagccacaag ggccagatga gggtcagcgc cgagacgccc gcgatgatga
     2821 acaggatcag cgcgctcatg gacaggctca tcccgatggc gacgagcacg ccgcccgcga
     2881 acagggcctt gaccttgcca acgaattccc gcttggcaat ctccagttcc cggcggacga
     2941 ccccgccgac ctgactcagg atcgccgaaa cgatagaact gaccgagaaa ctgctgaaga
     3001 acgaacgcgc catggttcct ccaggtgacg taccactagt acaaccctac cgggtgcgtt
     3061 gaggcgatgg ggaggcggag ggaaaggggt acctgatcgg ccgggagcgg tggccaggcc
     3121 cgggctcgaa ccggggacct ttcgattttc agtcgaacgc tctaccaact gagctatctg
     3181 gccgaacgaa cgggcccggc cataggccgg acgcgtcctc cgcgaccccg acgggacttg
     3241 aacccgcgac ctccgccgtg acagggcggc gcgctaacca actgcgctac ggggccttgt
     3301 gcgaaacaag accgtgctac gaaaccagac cacgctgtga acccggacca cgcgcaagag
     3361 caaagcctat agtagccgtc ccgatgaggt ccgtctccac cggctctgcc actcacggct
     3421 ctgccactcg cctcgtaccc ccaacgggat tcgaacccgt gctaccgccg tgaaagggcg
     3481 gcgtcctagg ccactagacg atgggggctt gtgggcgccc gacgtcctgg cgccgtggac
     3541 ccgacacatc ctacgagtcg tggcgtccat tcaccaactt gcaggcgggc gggacacaat
     3601 ggccccatgg tccagatgac gcgcgccgag ttcgaagagg cggtggtcga cgcgctcgat
     3661 cagatccccg aggaactggc gaggctcatg gatgacgtcg tcgtcctggt cgaggacgac
     3721 tccccggcgg gcgggcccgt gcttctcggc ctctacgagg gcgtggccct gacggatcgc
     3781 gggaactcgt ggggccatgg cgaactcccc gatcgcatct tcatctaccg gaacccgacg
     3841 ctggcgatgt gccggaccgc caaggaggtg cgcgacgagg tcgccgtgac cgtggtccac
     3901 gagatcgcgc accacttcgg catcgacgac gatcggctgc acgagctcgg gtggggctag
     3961 gcgcgggtcg cgaggcgggt gcgccggatt cgcgtcgagg atgggagact tcgttccatg
     4021 cgaacgatct tcccttccgg tgacgactac gaggactact acggctactc gcgcaccgtg
     4081 cgcgtcggca accaggtctc ggtttccggg acgaccgccc gcgcgccgca cgaccacagc
     4141 acggatgcgc acgcccaggc ggtctccgcg ctcgacgtca tccgcggagc cctcgaggag
     4201 gcgggcgcca cgatggccga cgtgacccga accgttgcgt acatcacgtc gattgactac
     4261 gtcgatgctg tcgcgcgcgc ccacaaggag gcgttcaacg acatccggcc cgccgcgacg
     4321 tgcgtcgtgg tcacgtcact cgtcgtcccg cacctgctcg tcgagattca ggtggacgcg
     4381 gtcgtcccct cgtagtgcct ggggcgcggc cggacgacta ccccttcgga tgcgccttcc
     4441 acgccgaggc gaccatgtcg cgcacggggt gtcgcatcgc ccagcccagg tcgcgagccg
     4501 cgagtgtgcc gtccgcgacg atgcgcgccg ggtcgccggc gcggcgcggc cccgcggtcg
     4561 gcacgaggct cgtcccggac accttgagca ccgcgtcgat gagttcccgc acgctcgtgc
     4621 ccgtgcccga gcccaggttg tagaccggct ccagcgcgcg ccccgccgcg aaggcccgcg
     4681 ccgccgcggc gtgggcaagc gcaacgtcgc cgacgtcgat gtagtcgcgg acgcacgttc
     4741 cgtcgggggt gtcaaagtcg tcgccgttga tgacgggcgc gcggccctcg acgagcccgg
     4801 tgaagacccg tgggaggacg ttgtaggggc tcacgtcgaa gacggcggga tcggccgaac
     4861 cggcgacgtt gaagtacctc aggctcgttc cggcgagggg cgcctccatc tccgcggtcg
     4921 cgaggacctg gccgcggatg aggagctcgg ccgcgagctt ggattctcca tacggcgact
     4981 cgggagcggt gggcgtctcc tccgtcacca gctcctccct gggcgtgccg tagaccgccg
     5041 cggatccgga gaacacaatt cgcgcaacgc cgaccgactc catcgccctg agcagcgact
     5101 cagtgcccga aaggttctgg cgccacgtgt gcagcggccg cctgacggac tcgcccgcga
     5161 acttgaagcc cgcgcaatgg atgacgccgt cgatggcgtg ttcgcgcaac gcggcctcga
     5221 ccgccgaggt gtccagcacg ctcgcgcggg tgagcgcgac tccgtgcggg aggaactcct
     5281 cgcgtcccgt cgagaggtcg tccagaacgg cgacgccgaa gtccgcctcg agcaaggccc
     5341 gaaccacgtg agcgccgatg tacccggcgc cccccgtaac gagccacgac atgctggcgg
     5401 tctcgacgcg cgtcacaacg tctccctgcg tgcgccgtcg gaagggacga cggtgaagat
     5461 gtgagcctcg cggaaccccg aggcttcgaa cgcggagagg accgtggctt ccgcgagttc
     5521 gaacttctct ctggggacga gcgcgatggc ggatccgccg aagccgccgc ccgtcatgcg
     5581 cgcgccgatc gcgccggcct ccatggcgac ctcgacggca cgatcgagtt cgggcacgga
     5641 gatctcgaag tcatcccgca tggaggcgtg cgaggcgagc aacaagggac cgatggcgcg
     5701 cggtccgtcg ttctcgagcg tcgtcgccgc cgccaggacg cggtcgtttt cggtgacgac
     5761 gtggcggacg cgccggaagg tctcgtcgtc gagcacggcc tgggcgcgga ccatgtcggc
     5821 gacgctcagg tccctgaggg acgaaacccc gagcgcggcc gcgccgcggt cgcaggacgc
     5881 ccggcgctct gcgtagccgc ccgtggcatg ctcgtgggtc accctcgtgt cgaccaccat
     5941 gacgtcgagg ccgttctccg cgaagcacag gggaacgatt tcggagtgat tgtcgcggca
     6001 gtcgaggagc accccgtgac cagcctggcc gagcagggaa gcggtctgat ccatgatccc
     6061 ggtcggagcg cccacggcac cattctcggc gcgttggcac acacgagcca ggacatccac
     6121 gccgaggccc agttcccact cagcgtcgat agccacggcg acggcacact ccagggccgc
     6181 ggaggaggac aggcccgcgc ccagggggac gtcactcgcg acggcgatgt ccaggccgcc
     6241 gcgggacgcg acgtcgacgc cgtgctcgcg gagcgcccag acgaccccga agacgtacgc
     6301 cgaccagccc gcgagattct ccgggtcaag gtcatcgacg ccgagctcca cgaccgcatc
     6361 gttcagggtc gtcgtcaccc ggaggatccc atcgcttcgt ggcgcgaggg cgcaccaggt
     6421 ccgtcgttcg atcgcaaagg gtaggacgag gccgtcgttg tagtcggtgt gctcgccgat
     6481 gaggttgacg cgccctggcg ccgacgcgag aacggcgggc acatgtccaa aggtcgcggc
     6541 gaacgcgccc gacgccgccg cgccgcaggg ctcgggggcc gtcatcgcgc gcccgccttc
     6601 tgatccaccg tggcgagtgc ctcgcgaagg cgcccggccg ccgactctgg cgagacgtcc
     6661 gcgacccacg ctcccatcgc ggcctcggaa ccggcgagga acttcaactt gtcggccgca
     6721 cgccgcgggc tctcgatctt gaacatgaga cggatgtcgt cgccgcccac cagcggcgcc
     6781 tggtgccacg acgcgatgta cggcgtcggg ccgtcgtaca tcgcgtccac ggcgcccagc
     6841 agcctcggca ggagaagggc gagttcgtcg cgctcggcca gggaagtctg tgcgaggtcg
     6901 cggatgtcgc ggtgcggaag gacgtggatc tcgatgggcc agcgcgccgc gaacgggacg
     6961 aaggcgctcc agtgaacccc ggcgtgaagc atgcgggtcg aaaggcgttc gcggtcgagg
     7021 atgtcggcga agagggtcgg tccgtagcgc gcgacctccg cccggact
//
LOCUS       DASVKP010000038         6032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1327735, whole genome shotgun sequence.
ACCESSION   DASVKP010000038 DASVKP010000000
VERSION     DASVKP010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327735"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1037
                     /locus_tag="VF362_00810"
     CDS             69..1037
                     /locus_tag="VF362_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7589501.1"
                     /translation="MTLLPFMLVQIDYDIPIGIPHLLVIVIAAGFIAAGAIVRSRAGA
                     AGPTLAHATVGFRRRAGLGVIAAIGVGAFLLVTFNGGEGTPRAAPAQLKVVSWNLHYG
                     VTPGLTGGPAVDLDATADYIRAQDADVVLLQEVERGWILAGGTDLLEYLADSLHMYYA
                     YAGAHDRQFGNAILSRYPISDPDMIRLPYGAGPQGRSAIVGTVATSKGPIQFVSVHLQ
                     HKDDGATRVSEVEALLAALEPVPSRVVAGDFNDTPDSRAVALMLAAGYTSAQDALWHE
                     QDTYVGSDFNARIDYQFLQGVSASGFGIGDSGRSDHLNLAVTVSVP"
     gene            complement(1071..1730)
                     /gene="deoC"
                     /locus_tag="VF362_00815"
     CDS             complement(1071..1730)
                     /gene="deoC"
                     /locus_tag="VF362_00815"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019310513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX7589502.1"
                     /translation="MTLTRAGLAAYVDHTPLGADASHADVAAAVKEARALGAFAVCVG
                     PSFLPLADPGPLRIVTECAFPDGGQSSEGKANDAANAFWAGAHEVDMVIDVAAAQRGD
                     FGAVQADVAAVRAEVPDGRLLNVILETAALTDEQIVGAAAAAEWAGADYVRTSTGVHP
                     AGGATVHAVELLRETVGGRLGIKASGGIATTEEALALIAAGATRLGLAQTRAVLEGLP
                     S"
     gene            complement(1831..2718)
                     /locus_tag="VF362_00820"
     CDS             complement(1831..2718)
                     /locus_tag="VF362_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589503.1"
                     /translation="MFARAAERATRRLPEADRFALAKAVVDLDQETRGVMDRALASEA
                     PVRAILALARQWPSLTQAERTIATSPLPSADEGPVTLDGVPARQVDSTTCGAASMAVM
                     LMMGDPFVGLWVASGRRLGGYLPPEIRHIPSQARLDAVERRWGALQKSIHIQTVRRGL
                     LLLLPWPERLGTPPWRVNNSTRFAGVGFHGALVDDTDDTDVDALVSHASAALRDGIPV
                     PIYASGDSAKGIDTVIPRHVVLLVRRLAEGFVAYEPGSARLYAITDDQIREGGAGLQA
                     LGNWSHVAWMILPRARRRS"
     gene            complement(2858..3955)
                     /locus_tag="VF362_00825"
     CDS             complement(2858..3955)
                     /locus_tag="VF362_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019134937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7589504.1"
                     /translation="MTPDSRQAIPRPDFSAVVPAGGSGTRLWPLSLPERPKFLHDLRG
                     TGASLLQDTVARLAPVASRMMVVTGVAHAAAVHEQLPALGDDDIVAEPSPRDSMAAIA
                     LAAAILEHRHGAHVFGSFAADQVVLRPDELMRAIRIAIEAAQAGAVVTIGIEPLFPAT
                     GFGWILRGEPLADVDGAFRAEAFVEKPDLETAQAYLDGGRHYWNAGMFVARTDVLLGH
                     LARLQPTLHDGVRALAAAWDGPDRGASLQRVWPTLTTIAIDHAIAEPVAAEGGVAVVA
                     SDCDWIDVGDFAVLASIVEPDSDGVTRLNRSAPTRALDASGTMVIGGDRPVVIIGVDD
                     VTVVDTPEALLILGRASAQRVKDAASSLEGA"
     gene            4011..4385
                     /gene="sdhC"
                     /locus_tag="VF362_00830"
     CDS             4011..4385
                     /gene="sdhC"
                     /locus_tag="VF362_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX7589505.1"
                     /translation="MPGGSSLYKGGTGMWMWLAHRATGVAVFFFLLVHVTDTALVRVS
                     PEAYNQVIGLYKNPVMGLVESGLVAAIAFHALAGLRIISVDVWGWANRHQRSLAWVVW
                     ILWIIAMAGFLPRHLGHVFGGE"
     gene            4385..4864
                     /locus_tag="VF362_00835"
     CDS             4385..4864
                     /locus_tag="VF362_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HEX7589506.1"
                     /translation="MSAPAVVPPRFRRPPRSRTHAEKWSWLFMRVSGSLLIVLIFTHL
                     FVNLMTGNGISAIDFGFVAGKWANPLWQWWDAAMLVLAMIHGTNGMRLLVNDYARARW
                     VRRTLKSALGAAFALVVVLGSLVIFTFDPCPKGAVAADLNAEVCASILGTSTTTEAP"
     gene            4861..>6032
                     /locus_tag="VF362_00840"
     CDS             4861..>6032
                     /locus_tag="VF362_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7589507.1"
                     /translation="MTHHEYDVVIVGAGGAGMRAALESSPHARTAVLTKLYPTRSHTG
                     AAQGGMAAALSNVEDDNYEWHVYDTVKGGDYLVDQDAAEIMCREAIDAVLDLEKMGLP
                     FNRTEDGKIDQRRFGGHTREHGAAPVRRACYAADRTGHMILQTLYQQCVKQKVEFYNE
                     FYVLDLILSENPQKVKKGAKVSVTGVVALELATGEIHTFSAKSVVLATGGAGKVFKTT
                     SNAHTLTGDGMALALRAGLPLEDMEFFQFHPTGLYGLGILLSEAARGEGGILRNSEGE
                     RFMERYAPSIKDLAPRDMVSRAIAREVREGRGCGPKKDYVLLDLTHLPPEQIDAMLPD
                     ITEFARTYLGIEPHTEGIPTFPTAHYAMGGIPTTVTGEVLCNATETVDGLYAAGECA"
BASE COUNT          920 a         2200 c         2012 g          900 t
ORIGIN      
        1 ttcggccagc tccatccgcc gcccgcgcgg ggctccgtta cctcggggcc tcggccacct
       61 tcggcctaat gaccctcctg cccttcatgc tcgtccagat cgactacgac atcccgatcg
      121 ggatccccca cctcctcgtc atcgtgatcg ccgcggggtt catcgctgcg ggcgcgatcg
      181 tgcgctctcg cgcaggcgcc gcgggtccga cgctcgcgca cgccacggtc ggcttccggc
      241 gccgcgcggg ccttggcgtc atcgcggcca tcggggtcgg cgccttcctg ctcgtcacgt
      301 tcaacggcgg ggaaggcacg ccgcgcgccg cgcccgccca actgaaggtc gtctcctgga
      361 acctccacta tggagtcacc ccgggactca ccggaggacc tgcggtggac ctcgatgcga
      421 ccgcggacta catccgcgcg caggatgcgg atgttgtgct ccttcaggag gtcgagcggg
      481 gatggatcct cgcgggcggc accgaccttc tggagtacct cgccgactcc ctccacatgt
      541 actacgcgta cgcgggcgcg cacgaccggc agttcggcaa cgcgatcctg tcgcgatacc
      601 cgatctccga tcccgacatg atccgactcc cctacggcgc cggcccccag gggcgaagcg
      661 cgattgtcgg aaccgtggcg acgtcgaagg gccccatcca gttcgtctcg gtccacctgc
      721 agcacaagga cgacggcgcg acccgagtgt ccgaggtcga ggccctactc gcggcgctgg
      781 agcccgtgcc gtcgcgggtg gtcgccgggg acttcaacga caccccggac tcgcgcgccg
      841 tcgcgctcat gctggccgcg ggctacacca gcgcgcagga cgccctgtgg cacgagcagg
      901 acacctacgt gggttcggac ttcaacgcgc gcatcgatta ccaattcctc cagggcgtct
      961 ccgcgtcggg cttcgggatc ggcgactccg ggcgttcgga tcacctcaac ctcgcggtca
     1021 ccgtctcggt cccgtagcgg atacgagccc atcccggggg cgggcggggg ctaggaggga
     1081 agaccctcga gcacggcgcg ggtctgcgcg agccccaggc gagtggcgcc ggccgcgatc
     1141 agcgcgaggg cctcctccgt cgtcgcgatg ccccccgacg ccttgattcc gagcctcccg
     1201 cctacggtct cgcggaggag ttcgaccgcg tgaacggtgg cgcccccagc ggggtgcacc
     1261 cccgtcgacg tcctgacgta gtccgcaccc gcccattcgg cggcggcggc ggccccgacg
     1321 atctgctcat cggtgagggc cgcagtctca aggatcacgt tgaggaggcg cccgtcgggc
     1381 acctcggccc gcacggcggc gacatcggcc tgcacggccc caaaatcccc gcgctgggcg
     1441 gccgcgacat cgatgaccat gtccacctcg tgagcgccgg cccagaatgc gttcgctgcg
     1501 tcgttcgcct tgccctcgct cgactgaccc ccgtcgggaa acgcacactc cgtcacgatg
     1561 cgcaggggcc ccggatcggc gaggggaagg aagctcggcc cgacgcacac ggcgaaggct
     1621 ccgagcgcgc gagcctcctt caccgccgcc gcaacgtcgg cgtggctcgc gtccgcgccg
     1681 agcggcgtgt ggtcgacgta ggccgcgagc ccggcgcgcg tgagggtcac ccgaccagtc
     1741 tgccctagcc ctcgcgtccc ggcgcggagg cccggccgcc aaagcccgcg aggacggcgt
     1801 tcgccaactc ctcccggacc tcgggcggcg tcaggatcgc cttctcgctc gagggagaat
     1861 catccacgcc acgtgagacc agttgccgag cgcctggagg ccggcaccac cctcccggat
     1921 ctgatcgtct gtgatcgcgt agaggcgcgc gctccccggc tcgtatgcca cgaacccctc
     1981 cgcgaggcgc ctcaccagga gcaccacgtg acgcgggatg accgtgtcga tgcccttggc
     2041 cgagtctccg gaggcgtaga tcggcaccgg aatgccgtcg cggagcgcgg ctgaggcgtg
     2101 ggagacaagg gcgtcaacat ccgtgtcgtc ggtgtcgtcg acgagggcgc catggaaccc
     2161 gaccccggca aaacgggtcg agttgttgac ccgccagggc ggcgtcccga gtctctccgg
     2221 ccacgggagg aggagcagga gtccgcgccg aaccgtctga atgtggatgg acttctggag
     2281 cgctccccag cgtcgctcga cggcgtcgag ccgagcctgc gaggggatgt ggcggatctc
     2341 gggcggcaag tatccgccga gcctccttcc gctcgcaacc cagaggccga cgaatggatc
     2401 ccccatcatg agcatgacgg ccatgctcgc ggccccgcag gtcgtggagt ccacctgtcg
     2461 tgccgggacg ccgtcgagcg tcacggggcc ctcatccgcc gagggcagcg ggctcgtcgc
     2521 gatcgtcctc tccgcctgcg tcagcgacgg ccactggcgg gcgagcgcga ggatcgccct
     2581 gaccggggcc tccgaggcca gcgcgcggtc catgaccccg cgggtctcct ggtcgagatc
     2641 gacgacggcc ttcgccaggg cgaagcggtc cgcctcgggc agccgcctcg tcgcgcgctc
     2701 cgcggcgcgc gcgaacacgc tatcccgtcc gggcctgggt ttgcggtcgg caagggcctt
     2761 cgccctactt accgccgccg cgccgcccca ccgtcgcctc aggccaacgg cacgggccgt
     2821 gcgcgcaagg accgtggtga gttgccaggg catgagatca ggccccttcg agggaactcg
     2881 ccgcgtcctt gacgcgttgc gccgaggccc ggccaaggat cagaagcgcc tccggcgtat
     2941 ccacgaccgt cacatcgtcc acgccgatga taacgaccgg ccggtctccc ccgatgacca
     3001 tggtgcccga cgcatcgagc gctcgcgttg gtgcggaccg gttcaggcgc gtgaccccgt
     3061 cggaatccgg ctcgacgatg gaggcgagca ccgcgaagtc gccaacgtcg atccagtcgc
     3121 agtccgaggc gacgacggcc acccctccct ccgccgcgac cggctccgcg atcgcgtggt
     3181 caatcgcaat cgtcgtgagc gtcggccaca ccctctggag ggaggcccct cggtccgggc
     3241 catcccacgc cgcggcgagg gcccgcaccc cgtcgtgcag agtcggctga agccgcgcga
     3301 ggtggcccag gaggacatcg gtccgcgcca cgaacatccc ggcgttccag taatggcggc
     3361 ccccgtccag gtatgcctgg gccgtctcga gatcgggctt ttcgacgaac gcctccgcgc
     3421 ggaaggcgcc gtcgacgtcg gcgagcggct ctccgcgcag gatccatccg aatcccgtcg
     3481 cgggaaagag cggttcgatt ccgatcgtga cgacggcacc ggcctgcgcg gcctcgatcg
     3541 cgatccgaat ggcacgcatc agttcgtcgg gacgcaaaac cacctgatcg gccgcgaacg
     3601 agccgaagac gtgcgcgccg tggcggtgtt cgaggatcgc cgccgcgagc gcgatcgccg
     3661 ccatcgagtc ccgcgggctg ggctcggcga cgatgtcatc atcgccgagc gcggggagtt
     3721 gctcgtgaac cgccgcggcg tgggcgacgc ccgtgacgac catcatgcga ctcgcaaccg
     3781 gggcgaggcg cgcgaccgtg tcctgcagca ggctcgctcc cgttccgcgc agatcgtgga
     3841 ggaacttcgg ccgctctggc agggataggg gccacagccg ggtgccggag cctccagccg
     3901 gaacgacggc ggagaagtcg gggcggggga tcgcttgcct ggaatcgggg gtcacagact
     3961 ccagactaga atggaaccca cgaccgcaca gcccatggag ggaaggaacc gtgcccggag
     4021 ggtcaagcct gtacaagggc ggcaccggaa tgtggatgtg gctcgcccat cgggctaccg
     4081 gtgtcgcggt cttcttcttc ctgctagtcc acgtcaccga caccgccctc gtgcgagtgt
     4141 cgcccgaggc ctacaaccag gtgatcggcc tctacaagaa ccccgtcatg gggctcgtcg
     4201 aatccggcct ggtcgccgcg atcgccttcc acgccctcgc cggacttcgc atcatctccg
     4261 tggacgtctg ggggtgggcg aaccgtcacc agaggagcct cgcctgggtc gtgtggatcc
     4321 tctggatcat cgcgatggcc ggcttccttc cccgccacct cggccacgtg ttcgggggcg
     4381 agtgatgtcc gcgcccgccg tcgtgccccc gcgcttccga cgcccccctc gctcgcgcac
     4441 ccacgcggag aagtggtcgt ggctcttcat gcgggtctcc ggctcgctcc tcatcgtcct
     4501 gatcttcacc cacctcttcg tcaacctgat gaccgggaac ggcatcagcg cgatcgactt
     4561 cggcttcgtc gcgggcaagt gggctaaccc cctctggcag tggtgggacg cggcgatgct
     4621 cgtcctcgcg atgattcatg gcaccaacgg catgcgcctt cttgtcaacg actacgcgcg
     4681 cgcccgatgg gttcgcagga ccctcaagtc ggccctcggg gcggcgttcg ccctcgtcgt
     4741 cgtgctcggt agcctcgtca tcttcacgtt cgacccatgc ccgaagggcg ccgttgcggc
     4801 ggacctcaac gcggaggtgt gcgcgtccat cctcggcacc tcgaccacta cggaggcccc
     4861 gtgacccacc atgagtacga cgtcgtcatc gtgggagccg gcggcgccgg gatgcgcgcg
     4921 gccctcgagt catccccgca cgccaggacc gccgtcctga cgaagctcta ccccacgcgg
     4981 tcccacacgg gtgccgcaca gggcggcatg gccgccgcgc tgtcgaacgt cgaagatgac
     5041 aactacgaat ggcacgtcta cgacaccgtc aagggtggcg actacctcgt cgaccaggac
     5101 gcggcggaga tcatgtgtcg cgaggcgatc gacgccgtgc tcgacctgga gaagatgggc
     5161 ctcccgttca accgcacgga ggacggcaag atcgaccagc ggcgcttcgg cgggcacacg
     5221 cgggagcacg gggccgcccc ggtgcgccgc gcctgctacg ccgccgaccg tacgggtcac
     5281 atgatcctgc agacgctcta ccaacagtgc gtgaagcaga aggtcgagtt ctacaacgag
     5341 ttctacgtgc tcgacctgat cctgtccgag aacccccaga aggtgaagaa gggcgccaag
     5401 gtctcggtga cgggcgtcgt ggccctcgag ctcgccacgg gggagatcca cacgttctcc
     5461 gcgaagtcgg tcgtcctcgc cacgggcggg gccggaaagg tcttcaagac gacctcgaac
     5521 gcccacaccc tcacgggcga cggcatggcg ctcgcgcttc gcgctggcct gccgcttgag
     5581 gacatggagt tcttccagtt tcacccgacg ggcctgtacg gcctcggcat cctcctgagc
     5641 gaggcggcgc gcggcgaggg cggcatcctt cgcaactccg agggcgagcg cttcatggag
     5701 cgctacgcgc cgtcgatcaa ggacctcgcc ccccgtgaca tggtctcgcg ggcgatcgcc
     5761 cgcgaggtgc gcgagggccg cggctgcggg ccgaagaagg actacgtcct cttggacctc
     5821 acccacctgc cgcccgagca gatcgacgcc atgctccccg acatcaccga gttcgcccgg
     5881 acctacctcg gcatcgagcc ccacacggag ggcataccga cgttcccgac ggctcactac
     5941 gccatgggag gcatccccac caccgtcacc ggcgaggtgc tgtgcaacgc caccgagacc
     6001 gtcgacggcc tgtacgcggc cggagagtgc gc
//
LOCUS       DASVKP010000039         3014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_998517, whole genome shotgun sequence.
ACCESSION   DASVKP010000039 DASVKP010000000
VERSION     DASVKP010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998517"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VF362_00845"
     CDS             complement(<1..645)
                     /locus_tag="VF362_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589508.1"
                     /translation="MTIARGLQVIYAEPVAVSGTMPDPRAPVATTPTPSPTTHDTSDM
                     RVPDGWSIASSPGGSGAFAYAYDPAWTDLRNSPEDRQFQKDAFAAAGDRPEGAVFLLG
                     DWQSAASSTLPAVTIRVVGDPVAGREGDLKAVVEQYAVERAAVEGDDNPEFFYSGEYA
                     HPLGYEGWGVRVWAHVDGENIAIDVTGVRYGDAVVFASMTADGHPVPIDPVPGVR"
     gene            1049..>3014
                     /gene="pflB"
                     /locus_tag="VF362_00850"
     CDS             1049..>3014
                     /gene="pflB"
                     /locus_tag="VF362_00850"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEX7589509.1"
                     /translation="MTTTANVSTLEDEDDGVAAVSGAWRGFAPGAWQDAIDVRDFIQC
                     NYTQYDGDASFLAGPTERTLGIWSHLSAMFPEERERGVYDIDAKTPSSITAHAPGYIN
                     KDSEVIVGLQTDAPLKRAIMPNGGWRIVAKQLQTYGFEVDPTLEKIFTEYRKTHNAGV
                     FDVYPPNVRAARSSHIITGLPDAYGRGRIIGDYRRVALYGVDALIHGKQVERLELDME
                     RSVEDIIRDREENAEQIRALGELGQMAASYGFDISGPASTGREAVQWLYFAYLGAVKE
                     QNGAAMSLGRTSTFLDIFLQRDIAAGVLTEGEAQEIIDDFVIKLRIVRFLRTPEYDTL
                     FSGDPTWVTESIGGMGDDGRSLVTKNSFRFLQTLYNLGPAPEPNLTVLWSEKLPRGFK
                     EFCAQVSIDTSAIQYESDELIRTSWGDDSAIACCVSAMCVGKQMQFFGARVNLAKSLL
                     YAINGGRDEINGKQVAPEAPAVVGDVLDFEDVLHKFDHLLDWLAETYVDALNCIHYMH
                     DKYAYERLEMALHDRDILRTMACGIAGLSVVADSLSAIKYATVRPVRDETGLVVDYDV
                     TGEYPTFGNDDDRVDAIASDLVTRFMGKIRRQATYRDALHTQSVLTITSNVVYGKHTG
                     NTPDGRRKGEPFAPGANPMNGRDNHGLLAAA"
BASE COUNT          507 a         1067 c          977 g          463 t
ORIGIN      
        1 acggaccccg gggaccgggt cgatcggaac gggatggccg tctgcggtca tggaggcgaa
       61 taccacggcg tccccgtagc ggactccggt gacgtcaatg gcgatgttct cgccgtcgac
      121 gtgggcccag accctcaccc cccagccctc gtagccgagg ggatgggcgt attcgccgct
      181 atagaagaac tcggggttgt cgtccccttc gacggcggcg cgctcgaccg cgtactgctc
      241 aacgacggcc ttgaggtctc cctcgcggcc cgcgaccggg tcgcccacta cgcgaatcgt
      301 caccgcgggc agggtggagg aggccgcact ctgccagtca cccaggagga agacggcgcc
      361 ttcggggcga tcgccggcgg cggcgaaggc gtccttctga aactgccgat cctcgggcga
      421 attcctcagg tcggtccacg cggggtcgta ggcgtatgcg aaggccccgc taccgcccgg
      481 cgaggaggcg atcgaccatc cgtccggcac ccgcatgtcc gacgtgtcgt gcgtcgtggg
      541 tgagggggtc ggggtcgtcg cgacgggggc ccgcgggtcc ggcatcgttc ccgagaccgc
      601 gacgggctcg gcgtagatca cctggaggcc gcgggcaatc gtcacgccgg cccacgctat
      661 gagagcgcga acgcctccca caatcacggt gacgagcacc cctatccggg ctcggcgacg
      721 cgtgatcgac caagaaggcc ccgcagggcg cgttctctca cgctgccggg tcccgtcgcg
      781 gggtgggttc ttgaaggggt cggtcagatc gcgctcgccg tcccaggggg ggagggtcat
      841 ggcgctccct tcgtctccga caacactagg gcagcggcct cggatccgcc gggtgcgcgt
      901 gagaacgtcg cgggccgaag gtccccgggt cgaaggtccc tcggtccatg gtcccgtcgg
      961 gaagggttag gggcaactta ccgttggtat gtccgaccga cactgcacga ccgccccaca
     1021 agggtgggta cctgggaggg aacccgcgat gacaactacc gcgaatgtca gcacgctcga
     1081 ggacgaggac gacggcgtcg cagccgtttc cggcgcctgg cgcggcttcg cccccggagc
     1141 ctggcaggac gccatcgacg tccgcgactt catccagtgc aactacaccc agtacgacgg
     1201 cgacgcctcc ttcctcgcgg gtccgaccga gcgcaccctg ggcatctggt cgcacctttc
     1261 cgcgatgttc cccgaggagc gcgagcgggg cgtctacgac atcgacgcga agacgccgtc
     1321 ctcgatcacg gcccacgcgc ctggctacat caacaaggac tccgaggtca tcgtcggcct
     1381 ccagacggac gcgccgctca agcgcgcgat catgccgaac ggcggatggc ggatcgtcgc
     1441 caaacagctc cagacctacg gcttcgaggt ggaccccacc ctcgagaaga tcttcaccga
     1501 gtaccgcaag acccacaacg ccggcgtctt cgacgtctac ccgccgaacg tgcgcgcggc
     1561 ccgctctagc cacatcatca ccgggctccc cgacgcctat ggccgcggcc gcatcatcgg
     1621 cgactaccgc cgcgtcgccc tctacggcgt cgacgccctg atccatggaa agcaggtcga
     1681 gcgcctcgag ctcgacatgg agcgttcggt cgaggacatc atccgcgatc gcgaggagaa
     1741 cgcggagcag atccgcgcgc tcggcgagct ggggcagatg gcggcctcct atgggttcga
     1801 catctcgggc cccgcttcca cgggccgcga ggcggtccag tggctgtact tcgcctacct
     1861 cggcgcggtc aaggagcaga acggagcggc gatgtccctc ggccgcacct cgacgttcct
     1921 cgacatcttc cttcagcggg acatcgccgc gggtgtgctc accgagggcg aggcccagga
     1981 gatcatcgac gacttcgtca ttaagctccg catcgttcgc ttcctccgta ccccggagta
     2041 cgacaccctc ttcagtggcg acccgacgtg ggtgaccgag tccatcggcg gcatggggga
     2101 cgacgggcgc tcgctcgtca cgaagaactc cttccgcttc ctccagaccc tgtacaacct
     2161 cggcccggcg ccagagccga acctcaccgt gctgtggagc gagaagcttc cccgcgggtt
     2221 caaggagttc tgcgcccagg tctcgatcga cacgtccgcc atccagtacg agtccgatga
     2281 gttgatccgc acatcgtggg gcgacgactc cgcgatcgcc tgctgcgtct cggccatgtg
     2341 cgtgggcaag cagatgcagt tctttggcgc ccgcgtcaac cttgccaaat cgctgctcta
     2401 cgcgatcaac ggcgggcggg acgagatcaa cggcaagcag gttgcccccg aggcccccgc
     2461 ggtcgtgggc gacgttctcg acttcgagga cgtgctgcac aagttcgacc acctgctcga
     2521 ctggctcgcg gagacgtacg tcgacgcgct caactgcatc cactacatgc acgacaagta
     2581 cgcgtacgag cgcctcgaga tggccctgca cgaccgggac atcctgagga ccatggcgtg
     2641 cggcatcgcc gggctttccg tcgtcgccga ctctctctcg gcgatcaagt acgccaccgt
     2701 gcgcccggtt cgggacgaga cgggcctcgt cgtggactac gacgtcacgg gcgagtaccc
     2761 gaccttcggc aacgacgacg accgcgtcga cgcgatcgcc tccgacctcg tcacgcgctt
     2821 catgggcaag attcgccgcc aggcgacgta ccgcgacgcg ctccacacgc aatccgtcct
     2881 gacgatcacc tcgaacgtcg tgtacggcaa gcacacgggc aacacccccg atgggcgccg
     2941 caagggcgag cccttcgccc cgggcgccaa tccgatgaac ggccgcgaca accacggcct
     3001 gctcgccgcg gcgc
//
LOCUS       DASVKP010000040         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1080493, whole genome shotgun sequence.
ACCESSION   DASVKP010000040 DASVKP010000000
VERSION     DASVKP010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080493"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..696
                     /gene="gmk"
                     /locus_tag="VF362_00855"
     CDS             142..696
                     /gene="gmk"
                     /locus_tag="VF362_00855"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX7589510.1"
                     /translation="MTRLVVLAGPTAVGKGTIVRRLVERYGDVWVSVSATTREPRPGE
                     RDGVDYYFVSDARFDEMIAEGELLEWAVVHGEHRYGTPRAPVLRHIAAGIPCILEIDL
                     SGARQVREAMPNATFVFVEPPSWEELVRRLELRGTEDEDERTRRLATARMEMAAAEEF
                     EHVVVNDDLDAAVDAVHALLVEER"
     gene            745..1011
                     /gene="rpoZ"
                     /locus_tag="VF362_00860"
     CDS             745..1011
                     /gene="rpoZ"
                     /locus_tag="VF362_00860"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX7589511.1"
                     /translation="MAGTIANPEGITYPPIDELLAKVDSKYALVVYSSKRARQINAYY
                     EALGEGHFENVGPLVEADTTDKPLSVALREINAGNLLELTKLPE"
     gene            1032..>1626
                     /locus_tag="VF362_00865"
     CDS             1032..>1626
                     /locus_tag="VF362_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEX7589512.1"
                     /translation="MTGGIAAYKVAIVLRRLVEDGHSVRVVPTRSSLHFVGRATWEAL
                     SGGPATSDAFSNVYGVEHIRLGQSADLVLVAPATADFLAQIANGEARELLGNVILATH
                     APVLVVPAMHTEMWLNPATQANVATLKARGVHVIEPAIGRLAGPDSGPGRLPEPEEIV
                     AAAYAVAAGDGLPLSAGTSAPWGDLAGLRVAISAGGTR"
BASE COUNT          251 a          563 c          572 g          240 t
ORIGIN      
        1 ccacgacctg ctccagtgcc ttcccggagt cgggcccagg cgcgcctccg cgaggatggg
       61 ggcggcgagc atcgcggcct cccggcgcat caggggcctt gggcagcacc aggtcgacgc
      121 gctcgtcaag ttggatcgct cgtgacgcgg cttgtggtgc ttgcgggtcc gacggcggtc
      181 ggcaagggaa ccatcgtccg tcgcctcgtg gagcgctacg gggacgtctg ggtgtccgtc
      241 tcggcgacga cacgggagcc ccgtccgggg gaacgcgacg gggttgacta ctacttcgtg
      301 tccgacgcac ggttcgacga aatgatcgcg gagggcgagt tgctcgagtg ggcggtggtt
      361 cacggcgagc atcgctacgg aaccccgcgg gccccggtcc tgcgccacat cgccgcggga
      421 attccctgca tcctcgagat cgacctctcg ggggcgcggc aggtacgaga ggcgatgccg
      481 aacgcgacgt tcgtgttcgt cgagccgccg tcgtgggagg aactcgtgcg gcggctggaa
      541 ctccgcggga ccgaggacga ggacgagcgc acgaggcgcc tcgctaccgc ccgcatggag
      601 atggccgcag cggaggagtt cgagcacgtc gtggtgaacg acgacctcga cgcggccgtg
      661 gacgcggtgc acgccttgct tgttgaggaa aggtagactt acccccgccg ccgggcgcct
      721 cgtccggtca gaatgggagt ggacgtggcc ggaacgatcg cgaaccccga gggcatcacc
      781 taccccccga tcgatgagct cctcgcgaag gtggactcca agtacgccct cgtggtctac
      841 agttccaagc gcgcccgcca gatcaacgcc tactacgagg cgctgggcga gggccacttc
      901 gagaacgtcg gacccctcgt ggaggcggac acgaccgaca agccgctgtc cgtcgcgctc
      961 cgcgaaatca acgcgggcaa cctgctcgaa ctgaccaagc tccccgagta gccatgcgcg
     1021 tccttctggg cgtgacgggt ggaatcgctg catacaaggt cgccatcgtc cttcgacgcc
     1081 tggtggagga cggccacagc gtcagggtcg tccccacgcg ttcctccctc cacttcgtcg
     1141 gccgcgccac gtgggaggcc ctctcgggcg ggcccgcgac gagcgacgcc ttctccaacg
     1201 tctacggcgt cgagcacatc cgcctcggac aatcggcgga cctcgtgctc gtggcaccgg
     1261 cgaccgccga cttcctcgct cagatcgcga acggcgaggc gcgcgagttg ctgggtaacg
     1321 tcatcctcgc gacgcacgcg cccgtcctcg tagtgcccgc gatgcacacg gagatgtggc
     1381 tgaaccctgc gacccaggcc aacgtggcca ccctcaaggc ccgcggcgtg cacgtcatcg
     1441 agcccgccat cgggcgcctg gctggccccg acagcgggcc gggtcgactt cccgagcccg
     1501 aggagatcgt cgccgcggca tatgccgtcg cggccgggga cgggctgccc ctgagcgcgg
     1561 gtacctccgc tccgtggggc gacctcgcgg ggctgagggt cgcgatcagc gccggcggaa
     1621 cgcgcg
//
LOCUS       DASVKP010000041         4823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_916288, whole genome shotgun sequence.
ACCESSION   DASVKP010000041 DASVKP010000000
VERSION     DASVKP010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916288"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="VF362_00870"
     CDS             complement(<1..1143)
                     /locus_tag="VF362_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7589513.1"
                     /translation="MVASKPVAAPTYGAENITVLEGLEAVRKRPSMYIGSTGERGLHH
                     LVYEVVDNSVDEALAGYCDTIVVTLLPEAGVRVVDNGRGIPVDIVESEGRPALEVVLT
                     VLHAGGKFGGGGYAVSGGLHGVGVSVVNALSTRLEAEVTREGHIWRQAYEIGVPVDPI
                     QKGEAAEGTGTTITFWANDTIFETTDYDYETLRSRFQQMAFLNRGLSITLIDERPEYA
                     VDDEADAEQRRTLTYRYDGGLVDYVDHLNDAKKSERVHAEIISFEAEDPGGAISLEVA
                     MQWTNAYSESVFTYANTISTTEGGTHEEGFRSALTSLVNKYAREKGILKDKDDNLTGD
                     DVREGLTAVLSVKLREPQFEGQTKTKLGNTEAKTFVQKVVGERLGDW"
     gene            complement(1279..1830)
                     /locus_tag="VF362_00875"
     CDS             complement(1279..1830)
                     /locus_tag="VF362_00875"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX7589514.1"
                     /translation="MTEPPTDDERGRPAKADGASQAREAMERARAGARARGATTRSAK
                     QASRARTSRGGGEVGREPYGSGREPRSVESEVAVLFEMPGWKENVQVAGVTARWRGVV
                     GDAIADHCSEVAFEAGIVRVRASSTAWATQLGTMAGHIRQLLNEAIGTEAVTEVRIAG
                     PTGRSWVKGHRTVRGRGPRDTYG"
     gene            complement(1811..2953)
                     /gene="recF"
                     /locus_tag="VF362_00880"
     CDS             complement(1811..2953)
                     /gene="recF"
                     /locus_tag="VF362_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX7589515.1"
                     /translation="MRVARLSLTDFRSYRELDLMLGPRVTTLVGPNGAGKTNIVEAIR
                     YLSILGSHRVSTDVPLIRAGAAAAVVRAKVERAGRAIVVEATIALGRAKGYRLGGNTA
                     KAREIAGLLRTVVFSPEDLDLVKGDPSGRRRFLDELCVALAPPLAGDLADYDRVVRQK
                     TALLKGARGRGSAPATLDVWDERQAEIGARIVRARLEAVTALAPHVSAAYADLSAGGE
                     SEFAYDSPSIDAPGPRDGAEDDLARRLLEAIGKARPKELERGVCLVGPHRDDLLVTLG
                     GMPARGYASHGESWSAALALRLGTYDLLTSASGPDSGEDGEPVLILDDVFAELDAARR
                     RALAERVAPAQQVIVTAAVPADVPASLVGDVLFVGEGRVSRDGAAD"
     gene            complement(2957..4084)
                     /gene="dnaN"
                     /locus_tag="VF362_00885"
     CDS             complement(2957..4084)
                     /gene="dnaN"
                     /locus_tag="VF362_00885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX7589516.1"
                     /translation="MKFSVDRDVLADAVAWTGRAVPSRPPTPVLAGVRIEADRSGVVR
                     LASFDYEVSAKAEFAAQVESEGVALVSGRLLREIANALPHKPVGFSLEGTKVSVTCGA
                     SRFTLATMPVDQYPNLPELPEQSGTVNGKDFETAVVQVASAASRDETLPILTGVRVEI
                     EGSNISLLATDRYRLALRELKWKPSVSNISAIALVRAKTLVETAKALGSGDVTVALSS
                     GNDIDLVGFAAGGLQTTSLLMDGDYPQVRRLFPDASPITATVKTQELMDATRRVSLVA
                     ERNSSVRLSFLAGEVTLDAGQTEDAQASEAIESVLDGDEITVAFNPQYLLDGLAAIST
                     EYVRFGFTQETKPVEFMGISKPGGELAPEFRYLLVPIRISS"
     gene            complement(4487..>4823)
                     /locus_tag="VF362_00890"
     CDS             complement(4487..>4823)
                     /locus_tag="VF362_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7589517.1"
                     /translation="VVLKDLISEDDSEITAATIIGKTAEYFGITMDELTGTSRSRVYV
                     TARQIAMYLCRQLTDLSLPKIGDHFGGKDHTTVMYAVKKVEDQIAQRRQMYNQVTEIH
                     ARIKQQSRS"
BASE COUNT          789 a         1660 c         1548 g          826 t
ORIGIN      
        1 ccaatcgccg agcctctccc ccacgacctt ctgcacgaag gtcttcgcct ccgtgtttcc
       61 gagcttggtc ttggtctggc cctcgaactg aggttcgcgc aatttgacgc tgaggacggc
      121 cgtcaatccc tcgcgaacgt cgtcgcccgt gaggttgtca tctttgtcct tgaggatgcc
      181 cttctccctc gcgtacttgt tcaccagcga ggtgagcgcg gaacggaacc cctcctcgtg
      241 ggtgccgccc tccgtcgtcg agatggtgtt cgcgtaggtg aagacggact cggagtaggc
      301 gttcgtccac tgcatcgcga cctcaagcga gatcgctccc cccgggtcct ccgcctcgaa
      361 actgataatc tcggcatgca cccgctcgga cttcttcgca tcgttgaggt ggtcgacgta
      421 gtcgacgagg ccgccgtcgt accggtaggt gagcgtgcgg cgctgttcgg catctgcctc
      481 gtcgtcgacg gcgtactcgg gccgctcgtc gatcagggtg atgctcagtc cccgattgag
      541 gaaggccatc tgctggaacc gcgaccgcag ggtctcgtag tcgtagtccg tcgtctcgaa
      601 gatcgtgtcg ttcgcccaga aggtgatcgt cgtcccggtt ccctcggccg cctcgccctt
      661 ctggatcggg tccaccggga cgccgatctc atatgcctgg cgccagatgt gaccctcgcg
      721 cgtgacctcc gcctcgaggc gtgtcgacag ggcgttgacg accgaaacgc cgacgccgtg
      781 gagaccgccg gagaccgcgt agcctccgcc tccgaacttg cctccggcgt gaagcacggt
      841 caagacgacc tcgagggcgg gcctgccctc cgactcgacg atgtcgacgg gaatcccgcg
      901 gccgttgtcc acgacgcgca ccccggcctc cggaagcagc gtgacgacga tcgtgtcgca
      961 gtatccggcg agagcctcgt ccacggagtt gtcgaccacc tcgtagacca ggtgatggag
     1021 ccccctctcg cccgtcgagc cgatgtacat gctcgggcgc ttccgaacgg cctcaaggcc
     1081 ctcgagaacg gtgatgttct ctgcgccgta agtgggcgcc gctacaggct tcgatgcgac
     1141 cacgaaaacg ccccttcaag cgagtggcgc ggccgaagac ggcgcgcgcg atgcagaacc
     1201 ccattctagt cgccccaggg ggcccccacg cgtcattggc aggcaattct cgattgaccg
     1261 accccacgga actttcgact agccgtacgt gtcccggggg ccccggccgc gaacggtcct
     1321 gtgtcccttc acccacgaac gccccgtggg gccggcgatc cgcacttccg tgacggcttc
     1381 ggtgccaatc gcctcgttca ggagttggcg aatgtgcccc gccatcgtgc caagctgggt
     1441 ggcccacgcg gtcgaggacg cccgaacccg cacgatcccg gcctcgaacg cgacctcgga
     1501 acagtggtcc gcgatggcgt ctcccacgac gccccgccag cgcgccgtga cgcccgcaac
     1561 ctgcacgttc tccttccagc ccggcatctc gaacagtacc gccacctcgc tctcgacgct
     1621 tctgggctcc cggccggatc cgtacggctc ccgaccgacc tccccgcccc cacggctcgt
     1681 ccgcgctctc gacgcttgct tggccgaccg cgtcgtcgcg ccgcgggcgc gggcgcccgc
     1741 cctcgcccgc tccatcgcct cgcgcgcctg cgaggcgcca tccgccttcg ccgggcgccc
     1801 gcgctcgtcg tcagtcggcg gctccgtcac gactcaccct cccctcgccc acgaacagga
     1861 cgtcgccgac gaggctcgcc ggaacgtcgg ctggcacggc ggcggtcaca atcacctgct
     1921 gcgccggggc gacccgctcg gcgagcgccc gcctgcgcgc ggcgtcaagt tcggcgaaga
     1981 cgtcatcgag gatcagcacc ggctcgccat cttcgccgga gtcgggaccg ctggccgagg
     2041 tgaggaggtc gtacgttccc agccggagcg cgagggcggc ggaccatgat tcgccgtggc
     2101 tggcgtaccc ccgcgccggc atcccgccga gggtcacgag aaggtcatcc cggtgcgggc
     2161 cgaccagaca cacgccccgc tcgagctcct tcggtcgcgc cttgccgatc gcctccagca
     2221 gccgccgggc caagtcgtcc tcggccccgt cccgcggccc gggcgcatcg atgctcggag
     2281 agtcgtaggc gaattcggat tcgccgccgg cggagaggtc ggcatacgcg gccgagacat
     2341 ggggcgcgag cgccgtcacc gcctcgagtc gcgcccggac gatgcgggcg cctatctcgg
     2401 cctggcgttc gtcccatacg tcgagcgtgg ctggcgccga gccccgaccc cgcgccccct
     2461 tcaggagcgc cgtcttctgg cgaacgacgc ggtcgtagtc ggcgagatcc cccgcgaggg
     2521 gcggggcgag cgccacgcac agttcgtcga gaaagcggcg gcggcccgag gggtcgccct
     2581 tgaccaggtc gaggtcctcc ggggagaaca cgacggtccg caacaggccc gcgatctccc
     2641 gcgccttcgc ggtgttgcca cccagtcggt agcccttcgc cctccccagc gcaatggtcg
     2701 cctcgacgac gatcgcccgg ccggcccgtt cgacctttgc gcgcacgacc gcggcggcgg
     2761 cgccggcccg gatgagaggc acatccgtgg agacgcggtg gcttcccaga atggacaagt
     2821 accggatcgc ctcgacgatg ttggtcttgc ccgcgccgtt cggtcccacg agcgtcgtca
     2881 cccgtggtcc gagcatgagg tccagctccc ggtacgaccg aaagtcggtc agtgacaaac
     2941 gggcgacgcg catgcgctac gaggagatgc gaatgggcac caacaggtag cggaactcgg
     3001 gggcgagttc gccgcctggt ttcgaaatcc ccatgaactc gaccggcttg gtctcctgcg
     3061 tgaaaccgaa ccgcacgtac tcggtcgaga tcgccgcaag tccgtcgagg aggtactgag
     3121 gattgaaggc gaccgtgatc tcgtcgccat cgagcacgga ctcgattgcc tcggaggcct
     3181 gcgcatcctc cgtctggcca gcatccaagg tcacttcgcc ggccaagaat gacaggcgca
     3241 ccgaactgtt gcgctccgcc acgagggaca cacgccgagt tgcgtccatg agctcttgag
     3301 tcttgacggt cgcggtgatg ggcgaggcgt ccgggaacag tcgccggacc tgaggatagt
     3361 ccccgtccat gagcagggac gtggtttgaa gcccgcccgc agcaaatccg accaggtcga
     3421 tgtcgttccc cgacgacagc gcgaccgtga cgtcaccgga accaagggcc ttcgcggtct
     3481 ccactagcgt cttcgcgcga acgagcgcaa tcgcagagat gttcgacacg gacggcttcc
     3541 acttgagttc ccgtagcgcc aaccggtagc gatctgtcgc gagcagcgag atgttggagc
     3601 cctcaatctc gacccgaacg cccgtgagaa tcggcagtgt ctcgtcgcgc gaggccgccg
     3661 aggccacctg cacaacagcg gtctcgaaat ccttcccgtt gaccgtgccg gattgctccg
     3721 gaagctccgg gaggttgggg tactgatcga ccggcatggt ggccaaggtg aagcggctcg
     3781 caccacacgt cacggatacc ttcgtgccct ccagcgaaaa ccccaccggc ttgtgcggaa
     3841 gcgcgttggc aatctccctc agtagccgtc ccgagacgag cgctaccccc tcgctctcca
     3901 cttgcgccgc gaactcggcc ttcgccgata cctcgtagtc gaaactcgcg agcctcacga
     3961 cgccgctgcg gtccgcctcg atccgcaccc cggcgagcac cggcgtcggc ggccgcgacg
     4021 gtaccgcgcg cccggtccac gcgacggcgt cggcgagtac gtcccggtca acggaaaact
     4081 tcatcggtca ctcctcggtt cgcttggcca cagactacgc caaacggatc acggctcgct
     4141 ggtccaaaag tgtgtatctc cgatcgagaa ccgccggcga tgacggatcg gagatcttgg
     4201 gagagaagag aaggaaggag ccgtagtagc gcctgtggaa acgggggata acggtcatcg
     4261 ttcctggtag agatggtggg tggttgggga aaacgccagg aagcgcggga tggtgggagg
     4321 ttggcggggg aagggtggtg gacagtgtcg ccagcaacgg gacgttgccc acaccccatc
     4381 cccaggaaaa ggggaggcaa ccaacagcca ttcacagggt tgtacacagg tgtggaattc
     4441 tcgcgtgaca tttgtcacgc gagatggcaa ggaagggtgc gggaattcag gagcgtgatt
     4501 gctgcttaat gcgcgcgtgg atctcagtga cttggttgta catttgccgg cgttgagcaa
     4561 tctggtcttc caccttcttc acggcgtaca tgaccgtggt gtggtccttg cccccgaagt
     4621 gatctccgat ttttggaagg gaaaggtccg ttagttggcg gcagaggtac atcgcgatct
     4681 ggcgcgcggt cacgtagacc cgcgagcgtg aggtcccggt cagttcgtcc atcgtgatgc
     4741 cgaagtattc cgcggtcttc ccgatgatgg tcgccgctgt gatctcggag tcgtcctcgc
     4801 tgatcaggtc cttgagcacc act
//
LOCUS       DASVKP010000042         3112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1492177, whole genome shotgun sequence.
ACCESSION   DASVKP010000042 DASVKP010000000
VERSION     DASVKP010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492177"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..485
                     /locus_tag="VF362_00895"
     CDS             165..485
                     /locus_tag="VF362_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX7589518.1"
                     /translation="MWYVYDGNIPLDATRPAGRLATVVDIFATVDDDGPLGWQERSLC
                     AQTDPEAFFPEKGGSTREAKKVCGSCEVRSECLDYALAHDERFGIWGGLSERERRKLK
                     RRAV"
     gene            510..>3112
                     /locus_tag="VF362_00900"
     CDS             510..>3112
                     /locus_tag="VF362_00900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7589519.1"
                     /translation="MSPTTPFVRALVVTHGRPEFLAETLACLAAQSRPVDSAFVVVSG
                     RALEDDDNIVVPPGMAVEIVRVKGSTFGKAVDGLLEGVEEREGEWLWLLHDDSAPDPD
                     ALEHLLAQAAKRRLAGIVGAAQVRWDDPTRLVNIGTTVSRHGARRLGLAEGDDLDQGQ
                     HYEKEDVLAVGLAGAIVTRGVWKRLGGTDPAYGRFGDSADFCRRAWRAGYDVVIAPRA
                     RVRHAQASLAQRATPGNAAGDVRTTHASRRAAEWYHAIAWSPAWLGPILCLWCVASSL
                     ARAVLRLTASDLRLAVAELRVPLLLWARIGRLPASRSAIRRVSKGGETVEDPLLATAG
                     DVFRFLRTRELGAFEAWRAESRPSDVQHRELAALATRRRWTLGGLSVSMVAIAVALFG
                     TWFAPLARGEALAGAALGTTGVSTAELWLRSFTGWSDAGLGSGSLDGGFAALMLPFSL
                     VPGGLAMGIGLLLLLSPLLAALSAWFAAGAATRSLVARTLAAVFWGVWPALIQSVSDG
                     RVGAVIVHLVLPLFALAVARSIGVERRDRLADGAIFPPSRLGSPSAAAVASLALAVIT
                     VASPVLLLPLVLAVVVVALGSTVHWRFALTIPLPAVALQGPALAAAWHGWGAAGWFSP
                     LVREDGPALASASPSGWDLLWGVAQHPPLWPGFPGVGTVVLTYGAGVLLVAAALAALA
                     SGRAAGAVASGWTLALAGLAVAAVSARTVSVVAGPDGRSAANGWSGPGLSLMALGLLC
                     AACAAAPPGWTPGVAWRARPLRAVAGALVVGVLAIYVAGAVWPGRAFGGDVHPSVPTV
                     LPLVANLEQRSQPISRSLVLWQDDAGLVRYTVEARDGSTTLAGRGSTTWPAAPVGAAD
                     ASVLAPAIGTLA"
BASE COUNT          355 a         1084 c         1144 g          529 t
ORIGIN      
        1 ctccgttcgg tcgcggcgaa acacggggcg tcctcacggt gttttctcgc tttgggtcta
       61 ccctctgcgc tcgcggtggg gtaccgtcga cttgacggac ggggattaca ccgatgtaat
      121 tccgaatagc ggtgagaact ccgcgcaaac cggaggaggg ccacatgtgg tacgtctacg
      181 acggaaacat tccgttggac gcaacccgac cagcggggag actcgcgacg gtcgtcgaca
      241 tcttcgcaac ggttgacgac gatggtccgt tgggctggca ggaacgctcg ctgtgcgcgc
      301 agacggaccc cgaggcgttc ttccccgaaa agggcggatc gacgcgcgag gcgaagaagg
      361 tgtgcggatc atgcgaggtg cgatcggagt gcctcgacta tgcgctcgct cacgatgagc
      421 gctttggcat ctggggtggc ctgtccgagc gtgagcgccg caaactcaag cggcgcgctg
      481 tctagtcgct cggcggggga ggggcggaga tgagtccgac cactccgttt gttcgcgccc
      541 tcgtcgtcac ccacggtcgc cccgagttcc tcgccgagac cctcgcctgc ctggcggcgc
      601 aatcgcgacc cgtcgattcg gccttcgtgg tcgtctcggg gcgcgcgctc gaggatgacg
      661 acaacatcgt cgtcccgccc ggcatggccg tcgagatcgt gcgcgtcaag ggctcgacct
      721 tcggcaaggc ggtggacggg ttgcttgagg gggtggagga gcgcgagggc gagtggctgt
      781 ggctgctcca cgatgactcg gcccccgacc ccgacgcgct cgaacacctg ctcgcccagg
      841 cggcgaagcg tcggctggct ggcatcgtcg gcgcggcaca ggtgcgctgg gacgatccga
      901 cccgactcgt gaacattggc acgacggtct cgcgtcacgg ggcgcggagg ctcggcctcg
      961 ccgagggcga cgatctcgac cagggccagc actacgagaa ggaggacgtc ctcgccgtcg
     1021 gcctcgcggg cgcgatcgtg acgaggggcg tctggaagcg gctgggcggc acagatcccg
     1081 cctacgggcg attcggcgac agcgccgact tttgcaggcg cgcgtggcgc gcggggtacg
     1141 acgtcgtcat cgccccgcgg gcgcgcgttc ggcatgccca ggcctccctc gcgcagcggg
     1201 caacgccggg gaacgctgcc ggagacgtcc gcacgacgca cgcctcaagg cgtgccgcag
     1261 agtggtacca cgcgatcgcg tggtcgccgg catggcttgg cccgatcctc tgcctgtggt
     1321 gcgtcgcgtc ttcgctcgcc cgggccgtgc tccggctcac ggcgagcgac ctgcgactcg
     1381 cggtggcgga attgcgcgtg ccgcttctcc tttgggcgcg gatcgggcgg ctgcccgcct
     1441 ctcggtcggc gattcggcga gtctccaagg gaggggagac ggtcgaggat ccacttctcg
     1501 ccaccgcagg cgacgtgttt cgattcctgc gtacgcgcga gctcggcgcg ttcgaggcat
     1561 ggagggccga gagccgccct tccgatgttc agcaccggga gctcgccgcc ctcgcgaccc
     1621 gcaggcggtg gaccctcggc ggtctctccg tctccatggt cgcgatcgcc gttgcgctct
     1681 tcgggacatg gttcgcgccc ctcgcccgcg gcgaggccct cgcgggcgct gcgctcggga
     1741 cgacgggcgt ctcgacggcg gaactatggc ttcggtcctt cacggggtgg tcggacgcag
     1801 gccttggctc ggggtcgctc gacggcggtt tcgccgccct catgcttccc ttcagcctcg
     1861 tgccgggcgg gcttgcaatg ggaatcggcc tcctgctcct gctcagccca ctcctggccg
     1921 cgctctccgc gtggttcgcc gcgggtgccg cgacgcgctc gctcgtcgcg cggaccctcg
     1981 ccgccgtttt ctggggcgtg tggcctgcgc tcatccagtc tgtgagcgac ggccgggttg
     2041 gggccgtgat cgtgcacctc gtgctcccgc tcttcgccct agcggttgcg cggagcatcg
     2101 gggtcgaacg cagggaccgc ctcgcggacg gtgcgatctt cccgccttcg cgcctcgggt
     2161 cgcccagcgc cgcagcagtg gcgtccctgg ctctcgccgt gatcacggtc gcctcgccgg
     2221 ttctcctcct tccgctcgtc ctcgccgtcg tggtcgtcgc gctgggctca acggtgcact
     2281 ggcgattcgc gctcaccatt ccgcttcccg ccgtggccct tcagggcccg gcgctcgccg
     2341 ccgcctggca cggctggggc gcggcggggt ggttcagccc gctcgtgagg gaggacggtc
     2401 ccgcgctcgc gtcggcgtcg ccgagcggat gggacctgct gtggggggtc gcccagcacc
     2461 cgcccttgtg gcccggcttc cccggagtcg ggaccgtcgt gctgacgtac ggcgccggag
     2521 tcctcctcgt tgcggccgct ctcgccgcgc tcgcgagcgg ccgggcggcg ggcgcggtgg
     2581 cctctggctg gacgctcgcg ctcgcggggc tcgccgttgc ggccgtctcc gcacggaccg
     2641 tttcggttgt cgcggggccc gacggccgga gcgccgcgaa cggatggtcg gggcccggac
     2701 tgtcgcttat ggcgctcggg ctcctgtgcg ccgcttgcgc ggccgcaccc cccggatgga
     2761 caccgggcgt cgcgtggcgc gccaggcccc ttcgcgcggt cgctggcgcc ctcgtcgtcg
     2821 gggtcttggc catctatgtt gcgggggccg tgtggcccgg gagggcgttc gggggggacg
     2881 ttcacccgag cgttcccacg gtgctaccgc tcgtggcgaa cctcgaacag cggtcccaac
     2941 cgatcagccg gtcgctcgtg ctgtggcagg acgacgccgg gctcgtgagg tacacggtcg
     3001 aggcgaggga cggctcgacg accctggcgg gacgtggctc cacgacgtgg cccgcggccc
     3061 cggtcggggc cgccgacgcc agcgtcctgg cacccgcgat cggaaccttg gc
//
LOCUS       DASVKP010000043         5092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_588582, whole genome shotgun sequence.
ACCESSION   DASVKP010000043 DASVKP010000000
VERSION     DASVKP010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588582"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VF362_00905"
     CDS             complement(<1..767)
                     /locus_tag="VF362_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7589520.1"
                     /translation="MKTPNKRLRIVAVLALGAGLAGTLSACSASTTTSGASATTAAAA
                     GGTYTLWDPYPNRDATSTWAQAIDACASTLGITIERNSGNTGDTVKDLTTAASNLPDI
                     AMVDNPKVSTLADAGLLTTAAENGLDVSSISANILSAGVLNGKTYGVPVGANTLGLYY
                     NPKVLTAAGVDIATVKDYASLTAALQKVVASGKKGITFSAVGTEEGSFQFLPWFWGAG
                     ADLTKIDSDKAVAALTLWTDWVKNGLAPNSVLQNTQGT"
     gene            complement(787..1851)
                     /locus_tag="VF362_00910"
     CDS             complement(787..1851)
                     /locus_tag="VF362_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7589521.1"
                     /translation="MANIGDVARVAGVSRSTVSYALSGKRSISSDTQFRIQSAIAELG
                     FTVNAGARALATAQTMTIGLLSQFHQDEFAPAMLQYIRSVCDVGRELGYDVLLVTDED
                     GASAVKRITSSNQVDGVVLLDVTHADPRVGPLRAARQPGALIGLPGDSRGLDVFDLDF
                     AESARMLVDHLHGLGHREIILISPPEHVFERGGAYGWRFRDAAIERAGRYGIRIHPHF
                     GESQQPAIGVNINAILDARKNATAMIVHNDATVAALPVVLQARGIRVPEDLSVASLYS
                     RDFGRIFSLPYTAVEGSSDKLGRLAVQKLVQRILDPDQAGPTVVKFIAPELMDRGSTT
                     YARPLRRVFAGLMSNRFDKE"
     gene            complement(1978..4056)
                     /locus_tag="VF362_00915"
     CDS             complement(1978..4056)
                     /locus_tag="VF362_00915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma and tau"
                     /protein_id="HEX7589522.1"
                     /translation="MNQALYRRYRPDTFADIVGQDHVTEPLMQALRSDRVAHAYLFSG
                     PRGCGKTTSARILARCLNCAEGPTDTPCGKCDSCVELARGGTGSVDVVEIDAASHNGV
                     DDARELRERAAFAPARDMYKIFILDEAHMVTPQGFNALLKLVEEPPPHIRFIFATTEP
                     EKVIGTIRSRTHHYPFRLVPPALLTDYLGTLCEAEKVSVASGVLPLVVRAGGGSVRDS
                     LSVLDQLMAGSGPEGVTYERAIALLGFTDAALLDDAVDALAAGDGASLFAVVERVIQT
                     GHEPRRFVEDLLERLRDLLVLSAAGEDGAKALGQVPLDQLDRMRDQARRLGLERGSRA
                     ADRVNDALTSMIGATSPRLHLELLCARLIAAETPQSRANLAQGRAGASAPDLRASAIA
                     ADAAGTDAPARPLSPLAALVVSNREPISGPVAPGSQATRPDAVGPTAIEPTVIEPTVI
                     EPAAFEPVVAEPVVAEPVVADAAGEGLLPETPSPPAAETAVDLPAPAAQEPSLPAPPL
                     VPPSAATGVPQEPSGAGDAELLRRRWAEVLGTLERRRVTWVLVSQSAQVARVEGTEVH
                     LAFASPQLAERFNGGQHAENVALAIRETLGLEVRVIGAAEGALTASPRGASTPTAPRA
                     TPTPLATPAPPAAPARTTEPLAATPSDPPRVAATEDEASDGDAYAPDAHLTGPEVIAT
                     MLGGTIVEGN"
     gene            complement(4105..4201)
                     /gene="ffs"
                     /locus_tag="VF362_00920"
     ncRNA           complement(4105..4201)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF362_00920"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4294..4381
                     /locus_tag="VF362_00925"
     tRNA            4294..4381
                     /locus_tag="VF362_00925"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4328..4330,aa:Ser,seq:gga)
     gene            4410..>5092
                     /locus_tag="VF362_00930"
     CDS             4410..>5092
                     /locus_tag="VF362_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX7589523.1"
                     /translation="MDKPTSSTSKFKTRREILRRHRRERQVLFFGIVTIALGAVGYGA
                     YSIYTGTMAGPFSAPFVTKAADFKSTITLPCPPADALPMATGDVLVRVFNGTDRAGLA
                     GAVLTDLTGRGYYGGGAGNWSRTYAGTARISFGVDGIREAYTVARNFVNPEFILDTRP
                     GVIVDVVVGADYSGELVALADPSLDPTVPLEAPGPCEKARLITPEPAPKHIPVDPRVS
                     ASPSPSPSP"
BASE COUNT          831 a         1657 c         1791 g          813 t
ORIGIN      
        1 gtgccctgcg tgttctgaag gacggagttc ggggcgagcc cgttcttcac ccagtcggtc
       61 cacagcgtaa gcgccgcgac agccttgtcg gagtcgatct tggtaaggtc agccccagct
      121 ccccagaacc acggaaggaa ctggaacgat ccttcctcgg tgccgacggc ggagaaggta
      181 atgcccttct tgcccgacgc cacgaccttc tggagtgccg cagtcaggga cgcgtagtcc
      241 ttgacggtgg cgatgtcgac gcccgcggca gtaagaacct tggggttgta gtacagaccg
      301 agtgtgttag cgccgacggg aacaccgtaa gttttgccgt tgagcacgcc agcgctaagg
      361 atgttcgccg agatgctcga gacatcgaga ccattctcgg cggccgtcgt cagaagaccg
      421 gcatccgcga gcgtggatac cttggggttg tcaaccatcg cgatatcggg aaggttcgac
      481 gcggcggtcg taaggtcctt gaccgtgtcg ccggtgttgc ccgagttgcg ctcgatcgtg
      541 atgccgagcg tcgacgcgca ggcgtcaatg gcctgtgccc acgtcgaggt ggcgtcgcgg
      601 ttggggtacg ggtcccagag ggtgtacgtg ccgcccgccg ccgccgccgt cgtcgccgaa
      661 gcgccggaag tggtggtcga agcggagcac gcggacaggg tgccagcgag tccggcaccc
      721 aaggcgagga ccgcaacgat gcggagcctc ttgttcgggg ttttcacaat tatcctcctg
      781 ttggtttcat tccttgtcga accggttaga catcagccct gcgaaaaccc gccgaagcgg
      841 gcgcgcgtag gtcgtgcttc cgcggtccat caactccggc gcaatgaact tcacgacggt
      901 cggccctgcc tgatccggat cgagaatcct ttgcaccaat ttctggaccg caagtcggcc
      961 caacttatcg gacgaaccct ccaccgccgt gtagggcaga gagaagattc gcccgaagtc
     1021 tcgggagtag aggctcgcca ccgacaagtc ctcggggacg cgaataccgc gagcctggag
     1081 cacgacgggg agagcggcga cggtcgcgtc gttgtgaacg atcatagccg tcgcgttctt
     1141 gcgggcatca aggatcgcgt tgatgttaac gccgatcgcg ggctgttgcg actcgccgaa
     1201 gtgagggtgg atccgaatcc cgtagcgacc tgcccgttcg atggctgcat ctcggaagcg
     1261 ccatccgtag gccccgccgc gctcgaagac gtgctcgggc gggctgatca ggataatttc
     1321 cctgtgccca aggccgtgaa ggtggtcgac cagcatccgc gccgactcag cgaagtccag
     1381 atcgaagaca tcgagccccc tggagtctcc gggaaggccg ataagggcgc ccggctgacg
     1441 ggcggccctc aacgggccga cgcgtgggtc cgcgtgcgtg acgtcaagga gaacaacacc
     1501 gtcgacctgg ttcgacgacg tgatgcgctt gacggcgctc gcgccatcct catcggtcac
     1561 cagcagcacg tcgtatccga gctcgcgccc aacgtcacag accgaccgaa tgtactggag
     1621 catggcgggc gcaaactcat cctgatggaa ttgcgaaagc aacccaatcg tcatcgtctg
     1681 cgccgtcgcg agcgcccgag cacccgcatt gacggtgaag cccagttccg cgatcgccga
     1741 ctgaatcctg aactgggtat cgctggaaat cgaacgcttg ccggagaggg catacgacac
     1801 cgtactgcgg gaaacccccg caacccgggc cacgtctcca atgttcgcca cagatcagcc
     1861 cctaatcgtc gaaccgcttc gacaagaagg tagcagtgcc cacgtcgaac tgcaagggcg
     1921 gtaatcgaac tgaaatgtaa ctggtaccga gactcgagcg gcgggaggga gtgatggcta
     1981 gttgccctcg acgatcgtcc cgccgagcat ggtggcgatg acctcgggcc cggtgaggtg
     2041 ggcgtccggc gcgtaggcat cgccgtcgga tgcctcgtct tcggttgcgg cgaccctcgg
     2101 agggtcggag ggtgtggccg cgaggggctc cgttgtccgc gcgggggccg cgggaggtgc
     2161 gggggtggcg aggggcgtgg gggtggccct cggcgccgtt ggggtcgacg cgccgcgcgg
     2221 cgaagccgtc agggcaccct cggccgcgcc aatgacgcga acctcgagcc ccagcgtctc
     2281 gcgaatcgcg agcgcgacgt tctcggcatg ctggcccccg ttgaagcgtt cggcgagttg
     2341 ggggctcgcg aaagcgaggt gcacttcagt gccctcgacc cgcgcgacct gcgcactctg
     2401 gctcaccagg acccacgtca ccctgcgacg ctcaagggtc cccagaacct cggcccacct
     2461 gcggcgcagt agttcggcgt ccccggcccc ggacggctcc tgcgggacgc ctgtggccgc
     2521 ggagggcgga acgaggggcg gtgcgggcag tgagggttcc tgggcggcgg gcgcggggag
     2581 gtcgacggcg gtctcggcgg cgggcgggga cggggtctcc ggtagaagcc cctcgcccgc
     2641 ggcatcggcc acgacgggct cggccacgac gggctcggcc acgacgggtt cgaatgcggc
     2701 aggttcgatc accgtgggtt cgatcaccgt gggttcgatc gcggtcggtc cgaccgcgtc
     2761 gggccgggtg gcctgcgacc caggcgcgac gggccccgaa attggctcgc ggtttgagac
     2821 gaccagggct gcgagggggc tgagcggtcg ggcgggggcg tccgttccgg ccgcatcggc
     2881 tgcgatagcg gacgccctta ggtccggggc ggatgccccc gcccgaccct gggcgaggtt
     2941 cgcgcgcgat tgaggagtct cggcggcgat gagcctcgcg caaagcaact caagatggag
     3001 tcgcggcgac gtcgcgccga tcatcgaggt gagtgcgtcg ttgacccggt cggcggcgcg
     3061 cgacccgcgc tcaagcccga gtcggcgagc ctggtcgcgc atgcggtcga gttggtcgag
     3121 cggcacctgg ccgagcgcct tcgcgccgtc ctctcccgcg gccgaaagaa ccagcaggtc
     3181 gcggaggcgc tcgagcaggt cctccacgaa tcgcctgggc tcgtggcccg tctggatgac
     3241 gcgctccacc acggcgaaga gcgaggcgcc atcccccgcc gcgagcgcgt ccacggcgtc
     3301 gtccagcagc gcggcgtcgg tgaagccgag gagggcgatc gcgcgctcgt aggtgacgcc
     3361 ctccggcccc gagcccgcca tgagctggtc gaggacggag agcgaatcgc gaacggatcc
     3421 gccgccggcg cgcacgacga gcggcaggac tcccgaagcc accgagacct tctccgcctc
     3481 gcacagggtc cccaggtagt cggtcagcag ggccggggga acgagccgga acgggtagtg
     3541 gtgggtccgc gagcggatgg tgccgatgac cttctccggc tcggtggtcg cgaagatgaa
     3601 gcggatgtgc ggcgggggct cctcgaccaa cttgaggagc gcgttgaacc cctgcggggt
     3661 caccatgtgc gcctcgtcga ggatgaagat cttgtacatg tcgcgtgccg gcgcgaaggc
     3721 cgcgcgctcg cggagttccc gcgcgtcgtc gacgccgttg tgggacgccg cgtcgatctc
     3781 gacgacgtcg acggatccgg tcccgccgcg cgccaactcg acgcacgagt cgcacttccc
     3841 gcagggcgtg tccgtgggcc cctcggcgca gttgaggcag cgcgcgagga tgcgggccga
     3901 ggtggtcttg ccgcatcccc gtggcccaga gaacaggtag gcgtgcgcga ctcggtcgga
     3961 tcgcagggcc tgcatgagcg gctcggtgac gtggtcctgc cccacgatgt ccgcaaacgt
     4021 gtccggtcga tagcggcggt agagggcctg gttcacacgg acgactctag tgcggccgcc
     4081 cgacgcctgc atcggcccgg acgcgaagga gaccccccac gcacccgcca gagcccacgt
     4141 acccttgctg catttctgcc ctgggggagt tccgcgaggt gacgccgcgt gaggggtcgc
     4201 gatcagtcta gccgcggcgc cctggcatca caggggctca gacgggaacc gaggtcgtat
     4261 ccgcgtcgtt gctccggtag catcggtgcc cctggaggat tcgcctagtg gcctatggcg
     4321 cacgcttgga aagcgtgttg ggtgaaagcc ctcgggggtt cgaatccccc atcctccgcc
     4381 aaggggcccg tctcgattag ggtggtgacg tggacaagcc gaccagttcg acgagcaagt
     4441 tcaagactcg tcgcgagatt ctccgccgac acaggcgcga gcgccaggtg ctgttcttcg
     4501 gcatcgtgac gattgcccta ggggcggttg gctatggggc ctactccatc tacacgggca
     4561 cgatggccgg gccgttcagc gcacccttcg tgaccaaggc cgccgacttc aagagcacca
     4621 tcacccttcc atgcccgcca gccgacgccc tgcccatggc cacgggcgat gtcctcgtgc
     4681 gcgtcttcaa cggcacggat cgcgcggggc tcgcgggagc ggtgctcacc gacctcacgg
     4741 gtcggggcta ctatggcggc ggtgccggga actggagccg tacgtacgcg ggcacggcgc
     4801 ggatcagttt cggagtggac ggcatccgcg aggcctacac ggtcgcgcgc aacttcgtga
     4861 atcccgagtt cattctcgac acgcggcccg gcgtgatcgt cgacgttgtc gtcggcgcgg
     4921 actacagcgg cgagctcgtc gcgctagcag acccatcgct cgatcccacg gtgcccctgg
     4981 aggctccggg accgtgcgag aaggcccgcc tgatcacgcc cgagcctgca ccgaagcaca
     5041 tccccgtcga tccgcgcgtg tcggcatcac cgtcgccatc gccgtcgccc ta
//
LOCUS       DASVKP010000044         3410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_425328, whole genome shotgun sequence.
ACCESSION   DASVKP010000044 DASVKP010000000
VERSION     DASVKP010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425328"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..547
                     /gene="rimI"
                     /locus_tag="VF362_00935"
     CDS             65..547
                     /gene="rimI"
                     /locus_tag="VF362_00935"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX7589524.1"
                     /translation="MTDPRGVSVRDLTSEDLGWVADQEKAIFGASAWSPEVIATDFAS
                     GFRRYRGLDIDGALAGYAVYGFDGDAFHLMNIAVRSDARGEGHAHLLMEDFLAEARRL
                     GVGEASLEVAVTNDAALALYRSHGFEDVRVRRRYYQPEGIDALVMRLRVTPASPTDRA
                     "
     gene            557..1597
                     /gene="tsaD"
                     /locus_tag="VF362_00940"
     CDS             557..1597
                     /gene="tsaD"
                     /locus_tag="VF362_00940"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015770924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX7589525.1"
                     /translation="MDPLVLGIESTCDETGVALVRGLDLLGEAVASSMDEHARFGGIV
                     PEVASRAHLEEMIPTIEAALGSAGLGLRDVDAIAVASGPGLVGSLTVGVAAAKALALA
                     LARPLYGVNHVVGHVAVDELVHGAFPERFLALVVSGGHSSLLMVDDIATGVRELGQTL
                     DDAAGEAFDKVGRLLGLPYPGGPHVDRLARGGDPTAIAFPRGLTAPKDFAKYPYDFSF
                     SGLKTAVARWIEACVDDGREVPAADVAASFAAAVADVLVTKTLRACDDHGVDTVVIGG
                     GFSANSQLRELAASRCEAAGVTVRIPPIRYCTDNGAMIAALGSAAVRRGVSPSDLGIG
                     VDSAMPLGVPVA"
     gene            1678..3243
                     /locus_tag="VF362_00945"
     CDS             1678..3243
                     /locus_tag="VF362_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7589526.1"
                     /translation="MAAALLLVVAGDAAAPAFRPPLYSGEPLVVRSATPDEIVLAWGP
                     TEGDLARGIFTARRMTLDEVAGQVIVALFGGQDPASVEELVRTYHLGGVAFLGGNVGS
                     GELTQQMTAGAQSGGDGRDWPVIVATDQEGGTVARLKNVIPALPAFLASGAARDKSVV
                     RSAYMGEGADMLALGFNMDFAPVADLTVGLADPIIRTRSAGDDPGDVSATVIAASDGL
                     LGAGVIPTVKHFPGHGGVTVDSHLDLPVQAASTEVLESYDLIPFRAAVEAGVPAVMMS
                     HVAVAAWGGAPSSLDPAAYAYLRDELGFRGVVVTDALNMAAVAGRGEGGPAVAALNAG
                     ADIALMPSDTAAAHAQIVAAVESGLLSRARLDEAAARVITLMRWQASLPRGDADGDYA
                     HTLATAGIAVTSPLCGGPLVGRSVTISGGWPEERAALAAELAARGVGNGGGTTIRLLG
                     SPGGSGAADVVVAMDGPWGLPQSSATTYVGLFGRSAESFAALADVLTGAVAPTGRWPV
                     ALPGLTGTECGTG"
     gene            complement(3240..>3410)
                     /locus_tag="VF362_00950"
     CDS             complement(3240..>3410)
                     /locus_tag="VF362_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX7589527.1"
                     /translation="ARGVGRATIKKRGTAVSPEDLRRQLALAGDAEATIILTRVAGAQ
                     RVLIVEPVDATA"
BASE COUNT          426 a         1182 c         1273 g          529 t
ORIGIN      
        1 gcggggacct tggcatcgat cccctctacc tgcgccgacc cgacgttcac gggaccccgc
       61 catcgtgacg gatccgcgag gggtgagtgt cagggacctg acctcggagg acctcgggtg
      121 ggtcgcggac caagagaagg caatcttcgg agcgtcggcg tggtcgcccg aggtgattgc
      181 gacggacttc gcctcgggct tcaggcgcta ccgcgggctc gacatcgatg gcgccctcgc
      241 ggggtacgcg gtctatggat tcgacggcga tgcgttccac ctgatgaaca tcgcggtgag
      301 gtcggacgcg cggggcgagg ggcatgcgca cctcctcatg gaggacttcc tcgccgaggc
      361 ccggcggctc ggggtggggg aggcatcgtt ggaggtcgcc gtcacgaacg acgccgcgct
      421 tgccctgtac cggtcccacg gattcgagga cgtgagggtg cggcggcgct actaccagcc
      481 ggaggggatc gacgcgcttg tgatgcgcct gagggtcact cccgcgagcc ccaccgaccg
      541 agcctagagt tgtcacgtgg atcccctggt actcggcatt gagagcacgt gcgacgagac
      601 gggcgtcgcg ctcgtgcgcg gactcgacct tctcggcgag gcggtcgcga gctccatgga
      661 cgagcacgcg cggttcgggg ggatcgttcc cgaggtcgcg tcgcgcgcgc acctcgagga
      721 gatgatccca accattgagg cggcgctggg ctccgcgggc ctcggcctgc gcgacgtcga
      781 cgcgatcgcc gtggcctccg ggcccggcct ggtgggctcg ctcaccgtgg gcgtcgcggc
      841 ggccaaggcc ctcgccctcg cgctcgctcg gccgctctac ggcgtcaacc acgtcgtcgg
      901 ccatgtcgcc gtcgacgagc tggtgcacgg cgccttcccg gagaggttcc tcgccctcgt
      961 ggtatcgggt gggcattcct ccctcttgat ggtcgacgac atcgccaccg gggtgcgcga
     1021 actgggccag accctcgacg acgccgcggg ggaggccttc gacaaggtgg ggaggctcct
     1081 cggtcttccg tatcccggcg gaccgcacgt tgaccggctc gcgcgcggcg gcgatcccac
     1141 cgcaatcgcc ttcccgcgcg gactgaccgc gccgaaggac ttcgccaagt acccgtatga
     1201 cttctcgttc agcgggctca agaccgccgt cgcgcggtgg atcgaggcct gcgtcgacga
     1261 cggtcgcgag gtccccgccg cggacgttgc cgccagcttc gccgctgcgg tggccgacgt
     1321 cctggtcacc aagaccctgc gcgcctgcga cgaccacggc gtcgacaccg tggtgatcgg
     1381 cggagggttc tcggccaact cgcaactgcg cgagctcgcc gcgagtcgct gcgaggccgc
     1441 gggagtcacg gttcgcatcc cgccgatccg ctactgcacg gacaacggcg ccatgatcgc
     1501 ggcgctcggg agcgcggccg tgcgccgggg cgtttcgcca tccgacctcg gaatcggcgt
     1561 cgactcggcc atgccgctcg gggtgccggt cgcgtgaggc ctccgacggt cggagggggc
     1621 aggagcgccc tcgcccggct ggctcgccgg ctcggaggcc cgggtcttcg ctacggggtg
     1681 gcggcggcgc tcctcctcgt tgtcgcgggc gacgcggcgg cgcccgcctt ccggccgccg
     1741 ctgtattcgg gtgagccgct cgtcgtgcgc agcgcgactc ccgacgagat cgtcctggcc
     1801 tggggcccca ccgaggggga cctggcccgc gggatattca ccgcgcgccg aatgaccctg
     1861 gacgaggtcg cgggacaggt gatcgtcgcc ctctttggcg gccaagaccc cgcgagcgtt
     1921 gaggaattgg tccgcacgta ccaccttggc ggcgtcgcgt ttctgggcgg caacgtcggt
     1981 tccggcgaac tcacgcaaca gatgaccgcg ggggcgcaga gcgggggcga cggtcgggac
     2041 tggcccgtga tcgtggcgac ggatcaggag ggcggcaccg tggcgcggct gaagaacgtg
     2101 atccccgcgc ttccggcgtt tctggcctcg ggcgccgctc gcgacaagtc cgtggtgcgg
     2161 tccgcgtaca tgggcgaggg cgccgacatg ctcgcgctgg gcttcaacat ggacttcgcc
     2221 ccggtcgcgg atctcaccgt tggcctcgcc gacccgatca tccgcacccg ctcggcgggg
     2281 gacgaccccg gcgacgtctc cgccacggtg atcgccgcgt cggacgggct actcggagcg
     2341 ggggtgatcc cgaccgtcaa gcacttcccc gggcacgggg gggtcaccgt cgattcgcac
     2401 ctggatctgc cagtccaggc ggcctcgacc gaggtcctgg aatcctacga cctcattcct
     2461 ttccgcgccg ccgtcgaggc gggcgttccc gcggtgatga tgtcgcacgt cgcggtcgcc
     2521 gcgtgggggg gcgcgccgtc gtctctcgac ccggcggcct acgcgtactt gagggacgag
     2581 ctcggctttc ggggcgtcgt cgtcaccgac gcgctcaata tggcggccgt ggcgggcagg
     2641 ggcgagggag gccccgccgt ggcggcgctg aacgcgggcg ccgacattgc tctgatgccg
     2701 tccgataccg ccgcggcgca cgcccagata gtggccgccg tcgagtcggg cctgctgagc
     2761 cgcgcgcggc tcgacgaggc ggccgcgcgg gtgatcaccc tcatgcgctg gcaggcgagc
     2821 ctgccccggg gcgacgcgga cggcgactat gcccatacgc ttgcgacggc gggcatcgcg
     2881 gtcacgtcgc cgctgtgtgg cgggcccctc gttggccggt cggtgaccat ctctggtggc
     2941 tggccggaag agagggccgc tcttgccgcc gagctcgcgg cgcggggagt cgggaacgga
     3001 ggaggaacga cgattcgact ccttggctcg cccgggggat cgggggccgc ggacgtcgtg
     3061 gtggcgatgg acggaccgtg gggccttccc cagtcgtcgg cgacgacgta cgtcggcctc
     3121 tttgggcgca gtgcagagag tttcgccgcg ctcgcggacg tgctgacggg ggcggtggcg
     3181 ccgaccgggc gctggcccgt cgcgcttccc ggcctcacgg gaaccgagtg cgggaccggc
     3241 taggctgtcg cgtcgaccgg ttcgacgatg agcacccgct gggctcctgc caccctcgtg
     3301 aggatgatgg tcgcctcggc gtcgcccgcg agcgccaact gacgccggag atcctccggg
     3361 gacacggcgg tgccgcgctt cttgatcgtc gcgcgcccga cgccccgcgc
//
LOCUS       DASVKP010000045         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049 k141_94789,
            whole genome shotgun sequence.
ACCESSION   DASVKP010000045 DASVKP010000000
VERSION     DASVKP010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94789"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..>1626)
                     /locus_tag="VF362_00955"
     CDS             complement(112..>1626)
                     /locus_tag="VF362_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7589528.1"
                     /translation="EATTFGIDIFRIFDSLNDVEQMRPAIRAIREGGTGVAEAALCYT
                     GNLSDPNETLYTLDYYLRLADQLVGAGAHILAIKDMAGLLRPQAAHTLIEALRQRFDV
                     PLHLHTHDTAGGQLATLLAASEAGVDAVDVANAAMAGTTSQPPMSALVAALEHTERDT
                     GISLAACEDMEPYWESVRRLYLPFESGLQGPTGRVYRHEIPGGQLSNLRQQAKGLGLG
                     DRFEEIENMYAAVDRILGRLVKVTPSSKVVGDLALQLVGTKADVRDFEENPGSYDLPE
                     SVIGFLEGELGVPPGGWPEPFRTKALAGRKPSHREHHLTKDDHEVLAKPGIRRRRRLN
                     HLLFPGPSREFDAATTAYGDISVLDTYHYLYGMTPGGAEEISIDVEKGVKMLVTLEAI
                     GEPDDHGMRTLIFSHNGQIRPVGVRDETADVHVKTRKKADPSIPGDIAAPFTGAVTAS
                     VEEGATVSAGDTVATIEAMKMEAAITTPVAGTVKRVLISHTETLQGGDLVLVVQ"
BASE COUNT          227 a          527 c          579 g          293 t
ORIGIN      
        1 cgtacttgga gccaacggca acaagcacct tcatgggcgg cctcctttgg acacccttca
       61 tcgtcctacg cacttgcgcg cgcccatggg gacgaaggtc ccttggtgga cttactggac
      121 gaccaggacg agatctccgc cctgaagcgt ctccgtgtgg ctgatcagga cgcgcttgac
      181 cgttccggcg accggggtcg tgatcgcggc ctccatcttc atggcctcga tcgtcgcgac
      241 ggtgtcgcca gccgagaccg tcgcgccttc ctcgacggaa gcggtgacgg cgccggtgaa
      301 cggggcggcg atgtcacccg gaatcgaggg gtcggccttc ttccgggtct tgacgtgaac
      361 gtccgccgtc tcgtcccgca cgccgacggg tcggatctgg ccattgtggc tgaagatgag
      421 cgtccgcatc ccgtggtcgt cgggctcgcc gatcgcctcg agcgtgacga gcatcttcac
      481 gcccttctcc acgtcgatgc tgatctcctc cgccccgccg ggtgtcatcc cgtagaggta
      541 gtgataggtg tcgagcaccg agatgtcgcc gtacgcggtg gtggcggcgt cgaactccct
      601 ggacgggccg gggaagagga ggtgattgag cctcctgcgc cgcctgatgc cgggcttcgc
      661 gaggacctcg tggtcgtcct tggtgaggtg gtgctccctg tggctgggct tgcggccggc
      721 gagcgccttg gtgcggaagg gctcgggcca tccgcccggc gggacgccca gctccccctc
      781 gaggaagccg atgacgctct ccgggaggtc gtaggagccg gggttctcct cgaagtcccg
      841 gacgtcggcc ttcgtcccca cgagctggag ggcgaggtcc cccacgacct tcgaggatgg
      901 cgtcaccttg accagcctgc cgaggatgcg gtccaccgcc gcgtacatgt tctcgatctc
      961 ctcgaaacgg tcgccgagcc cgagcccctt cgcctgctgg cggaggttcg agagctggcc
     1021 gcccgggatc tcgtggcggt acacgcgccc ggtggggccc tggaggccgc tttcgaaggg
     1081 aaggtagagg cggcggacgc tctcccagta gggctccatg tcctcgcacg cggcgaggga
     1141 aatccccgtg tcgcgctcgg tgtgttcgag cgccgcaacg agggcgctca tggggggctg
     1201 agaggtcgtg cccgccatcg cggcgttcgc cacgtcgacg gcatcgaccc cggcctccga
     1261 ggccgcaaga agggtcgcga gttggccgcc cgccgtgtcg tgggtgtgaa ggtgaagcgg
     1321 aacgtcgaag cgttgcctca gcgcctcgat cagggtgtgt gcggcctgag ggcgcaagag
     1381 gcccgccatg tccttgatcg caaggatgtg ggcgccagcc cccacgagtt ggtcggcgag
     1441 ccggaggtag tagtcgagcg tgtacagcgt ttcgttcggg tcgctgaggt tcccggtgta
     1501 gcacagcgcg gcctcggcga cgccggtccc cccctcgcgg atcgcccgga tggcgggccg
     1561 catctgctcc acgtcgttga gcgagtcgaa gattcggaag atgtcgatgc cgaaggttgt
     1621 ggcctc
//
LOCUS       DASVKP010000046         2495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049 k141_94803,
            whole genome shotgun sequence.
ACCESSION   DASVKP010000046 DASVKP010000000
VERSION     DASVKP010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94803"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..676)
                     /locus_tag="VF362_00960"
     CDS             complement(194..676)
                     /locus_tag="VF362_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7589529.1"
                     /translation="MSAVPDARAGDTQAEELAGSLRTVVGRLSHRFRGVAAARGITPT
                     RLSALVALSKVGPMRPSDLATSLGITPASTSRLVEALESEGWVARTADPSDGRAFLLS
                     VSDAGSGVLAGLRREADGKLADAVRALTPDERRALADALPVLAAIADRYLAESSVPPR
                     "
     gene            complement(673..>2495)
                     /locus_tag="VF362_00965"
     CDS             complement(673..>2495)
                     /locus_tag="VF362_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7589530.1"
                     /translation="LTRADRAARDTAKPHYRWIVLSNTTLGVLMASINMSILMIALPD
                     IFRGVGIDPLQPGNTGLLLWLIMGYMVVTAVLVVGFGRLGDMFGRVRMYNAGFAVFTF
                     FSVMLAITWMHGTVAVMWMILMRVFQGVGGAMLMANSSAIITDAFPSHRRGLALGLNQ
                     VAGIAGSFIGLVVGGLLAPIEWHLVFVVSVPIGIFGTIWAYLRLHDTGIRRHATMDWW
                     GNATFAVGLIAVLVGITYGIQPYGTQTMGWMNPFVLTALIGGVITLVAFCVIETRVAE
                     PMFRLSLFRIRAFTFGNLASLLSSLGRGGLMFVLIIWLQGIWLPTHGYSFESTPLWAG
                     IYMLPLTAGFLVAGPVSGWLSDRFGARLFSTGGMLVAAGSFFWLLSLPVNFVYWQFAL
                     ALLLNGIGMGLFAAPNRASIMNSLPAHQRGVGSGMSTTFMNAAMVLSMGIFFSLMIAG
                     LAHSLPETLQAGLTAQGVPADAAAKVAALPPVGVLFASLLGYNPIQTLLGPTVLAQVP
                     VANAHFLAGQSFFPSLISGPFADGLAVAFGFAITACLVAAVASSLRGAKYVHDDAPDD
                     EAPDGGISDDDAREDGISDDEAREDATPGAAHREAVAASR"
BASE COUNT          439 a          808 c          938 g          310 t
ORIGIN      
        1 gcgacgacga cggtgccaag gcggcgagac attcggccct ccgatgtgat ggcgactccc
       61 acaacgaccc tagcggcgcc gccggcggcc cgcgcggcga gcgggtgaac cgggaccgtg
      121 accgcgggcg gctaccgacc acagcccgat tggtcaacgt gttgaccatc gcgcgcatgg
      181 tcggtggccc cgcctagcgc ggagggacgg aactctccgc gaggtaccgg tcggcgatgg
      241 ccgcgagcac ggggagcgca tccgcgagcg cccgacgctc gtccggcgtg agcgcgcgga
      301 ccgcgtcggc gagcttcccg tcggcctcgc gacgaagccc ggcgaggacg cccgaccccg
      361 cgtcgctgac gctgaggagg aacgcgcgtc cgtcgctcgg gtcggcggtg cgcgcgaccc
      421 agccttcgga ttcgagcgcc tcgaccaggc gcgacgtgct cgccggggtg atgccgagcg
      481 acgtcgcgag gtcgctcggg cgcatcgggc ccactttcga gagcgcgacg agcgcgctca
      541 gtcgcgttgg cgtaatgcct cgggcggcgg caacgcccct gaatcggtgc gacagccgtc
      601 cgacgaccgt gcggagcgat ccggccagtt cctccgcttg cgtatcgccc gcccgcgcgt
      661 cggggaccgc gctcatcgcg aggccgccac ggcctcgcga tgagcggcgc ccggagtcgc
      721 gtcttcccga gcctcgtcgt cggaaatccc gtcttcgcga gcgtcgtcgt cggagatccc
      781 gccgtcggga gcctcgtcgt cgggagcgtc gtcgtggacg tactttgctc cgcgcagcga
      841 ggacgcgacg gcggcgacga ggcacgccgt gatcgcgaac ccgaacgcga ccgcgaggcc
      901 gtcggcgaag gggcccgaga tgagcgacgg gaagaacgac tggcccgcga ggaagtgggc
      961 gttcgccacg gggacctgcg cgaggaccgt cggccccagg agcgtctgga tggggttgta
     1021 ccccagcagc gacgcgaaca gcacgccgac gggaggcagg gccgcgacct tcgccgcggc
     1081 gtcggcgggg acgccctggg cggtgaggcc ggcctggagc gtctcgggaa gggagtgcgc
     1141 gagcccggcg atcatcagcg agaagaagat ccccatcgac aggaccatcg ccgcgttcat
     1201 gaacgtggtg ctcattccgg agccgacgcc gcgctggtgg gcgggcaggc tattcatgat
     1261 gctcgcccgg ttgggggcgg cgaacaggcc catcccgatg ccgttgagca ggagggcgag
     1321 cgcgaactgc cagtagacga agttgaccgg gagggagagg agccagaaga aactcccggc
     1381 ggcgacgagc atgcccccgg tggagaagag gcgcgcgccg aagcggtcgg agagccaccc
     1441 ggacacgggc cctgcgacga ggaagcccgc cgtgagggga agcatgtaga tgcccgccca
     1501 caggggggtg gactcgaacg agtacccgtg ggtgggaagc cagatgccct gaagccagat
     1561 gatgaggacg aacatcagcc cgccgcggcc gagcgagctc agcaggctcg cgaggttgcc
     1621 aaacgtgaag gcgcggatgc ggaagaggct gaggcggaac atgggctccg cgacgcgggt
     1681 ctcgatgacg cagaacgcga cgagcgtgat cacgccgccg atgagcgcgg tcagcacgaa
     1741 cgggttcatc cagcccatgg tctgcgtgcc gtacggctgg atcccgtacg tgatgccgac
     1801 gagcaccgcg atgagcccga cggcgaacgt cgcgttgccc caccagtcca tcgtcgcgtg
     1861 gcggcggatc ccggtgtcgt ggaggcgcag gtaggcccag atggttccga agatgccaat
     1921 cgggacggaa acgacgaaga cgaggtgcca ctcgatcggg gcgaggaggc cgccgacgac
     1981 gaggccgatg aacgagcccg cgatgccagc gacctggttc agtccgagcg cgaggccccg
     2041 acggtggctc gggaacgcgt cggtgatgat cgcgctcgag ttggccatga gcatcgcccc
     2101 gcccacgccc tgaaacactc gcatgaggat catccacatg accgcgacgg tcccgtgcat
     2161 ccaggtgatc gcgagcatca cggagaagaa cgtgaagacc gcgaagcccg cgttgtacat
     2221 gcgcacgcgg ccgaacatgt cgccgagcct gccgaacccc acgacgagca cggccgtgac
     2281 gaccatgtag cccatgatca gccacagcag caggccggtg ttgccgggct ggagcgggtc
     2341 gattccgacc ccacggaaga tatcggggag cgcgatcatc aggatggaca tgttgatcga
     2401 cgccatgagg acgccgagcg tcgtgttgga caggacgatc cagcggtagt ggggcttggc
     2461 ggtgtcgcgc gcggcgcggt cggcgcgggt gagcg
//
LOCUS       DASVKP010000047         6845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_671802, whole genome shotgun sequence.
ACCESSION   DASVKP010000047 DASVKP010000000
VERSION     DASVKP010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671802"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VF362_00970"
     CDS             complement(<1..217)
                     /locus_tag="VF362_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589531.1"
                     /translation="MASKRSSSSGGGDMQSMWWFLGALLLGIAEIFTLDLTLLMCAGG
                     ALAGAFAILLGAPQWLAASIAFVVAGLL"
     gene            complement(250..1044)
                     /locus_tag="VF362_00975"
     CDS             complement(250..1044)
                     /locus_tag="VF362_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7589532.1"
                     /translation="MTEVLRVERATLRREGNAVLTAVDWTVNEGERWVILGPNGAGKT
                     SLLTLAAARAHPSEGAVWVLGERLGTTAVQELGLRVGLASDAVAARIPGRELARDVVM
                     TAAHGVTGRWKEPYDLVDFERAGALMAAFGIQDFGGREYRTLSEGERKRVQLARALMT
                     DPEILLLDEPAAGLDLTGREELLEALTELAADPRSPVLILVTHHVEEIPRGFTHALLL
                     GGGRVVAAGPLSATITEANLSLAYGIPVELNESSGRWTARKKAIIP"
     gene            complement(1061..2257)
                     /gene="glgA"
                     /locus_tag="VF362_00980"
     CDS             complement(1061..2257)
                     /gene="glgA"
                     /locus_tag="VF362_00980"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEX7589533.1"
                     /translation="MRVDILTREYPPHVYGGAGVHVAELARVLRPRLDVRVRCFDGPR
                     DEPGVEGYVLGTSTGDAALDVLALGIPMARDCRGADLVHSHTWYGNMAGHLASLLHDV
                     PHVLTAHSLEPLRPWKAEQLGGGYRVSSWIERTAYEAAAGVIAVSEGMRADVLRVYPF
                     MDPTRVGVVHNGIDVEGWSAPVGDERVEAEALVRGYGIDPDRPAVVFVGRITRQKGLP
                     YFLQAVARLSPEIQVVLCAGAPDTPEIAAEVSGAVATLQETRSGVIWIEKILPRRELV
                     AVLDACTAFVCPSVYEPLGIVNLEAMAVGLPVVGTATGGIPEVVVDGETGVLVPIDQV
                     NDGTGTPIDPERFVADLASALETVATDPARAKVMGAAGKVRAAEHFSWEAIADRTVAV
                     YEALLA"
     gene            2344..3585
                     /locus_tag="VF362_00985"
     CDS             2344..3585
                     /locus_tag="VF362_00985"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX7589534.1"
                     /translation="MPNSSVLAIVLAGGEGKRLMPLTNSRCKPAVPFGGTFRLVDFAL
                     SNLINSRYLHVVVLTQYKSHSLDRHIARTWRMSALLGNYVAPVPAQQRRGKHWYLGSA
                     DAIYQCQNIIDDERPDIVIIVGADHVYRMDFSQMVDAHVASGAEFTVAGIRQPIEMAS
                     EFGVIDVDPKNPGHIRAFLEKPKNPQGLPDSPGEILASMGNYVASTDALLAALAADGA
                     DESSAHDMGGNIVPHFVERGACGFYDFRDNVVPGSTDRDRAYWRDVGTLDAYFEANMD
                     LIALEPIFNVYNKKWPVHQGLITQPPAKFVHSEEGRLGHAADSVVSPGVIVSGATVTG
                     SVLSPDVRLHSWSSVSDSVLLDHVIVNRHAQVRRAILDKNVVLEEGAAVGLDREHDIE
                     RGFTVTDSGITVVGKGVFVPA"
     gene            complement(3639..4145)
                     /locus_tag="VF362_00990"
     CDS             complement(3639..4145)
                     /locus_tag="VF362_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7589535.1"
                     /translation="MRVLVLARHAKAEAHTGALTDHERTLALRGRTDAKELGAALAAA
                     GFEPDVAYVSDSVRTSQTWELASTAWEVPVVHLRRELYATTVATLVGLLAGTEPGATN
                     VIVVGHEPTISAAALFLAGPGSSREAVDRVSGGLPTGMAVVLEFDGEWPGLAARSARL
                     VGVVGRTE"
     gene            complement(4151..4918)
                     /gene="fabI"
                     /locus_tag="VF362_00995"
     CDS             complement(4151..4918)
                     /gene="fabI"
                     /locus_tag="VF362_00995"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX7589536.1"
                     /translation="MGFLEGKNILITGVLTEGSIAFSVARLCQEQGATVVLTGVGRTM
                     KVTQSMSGRLPVEAPVVELDITNEHQLSALAHRVREHVPHLDGVVHSIGFAPQSVMGG
                     NFMSGKWEDVATAIHISAYSLKALAAATRPLMTGGGSIVGLTFDGRYAWPVYDWMGVA
                     KAAFEATSRYVARDLGPEGIRCNLVAAGPIMTTAAKHIPGFDQMESKWDERAPLGWDS
                     SDPEPTARAVTALLSDWFPATTGSVVHVDGGVHAMGQ"
     gene            complement(4983..5687)
                     /gene="fabG"
                     /locus_tag="VF362_01000"
     CDS             complement(4983..5687)
                     /gene="fabG"
                     /locus_tag="VF362_01000"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX7589537.1"
                     /translation="MARHVHITGANRGIGLAIAQRFLAAGDTVSTIYIGDAPPDPIDG
                     IVGDVRDSEAVNAAMDAFEAKHGPIDVLVANAGITKDGLLMRMTDEDFDAVIGVNLQG
                     AFRCVRRASTSMMKKKSGRIILIGSVVGLMGNPGQVNYSSSKAALVGMARSITREIGS
                     RGITANVIAPGFINTAMTAVLTDDVQKQYRDSIPARRFGEADEVAGAAFFLASDDAAY
                     ISGAVIPVDGGLGLGH"
     gene            complement(5768..>6845)
                     /locus_tag="VF362_01005"
     CDS             complement(5768..>6845)
                     /locus_tag="VF362_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX7589538.1"
                     /translation="GDSETLLLDEPTNHLDADSIVWLRDFLRSYSGGLVVISHDAGLL
                     RACANKVFHLDANRSELDQYSLGWDAYLQQREEDEKRRHRERDNAERKAEALIAQANK
                     MRAKATKAVAAQGMLRRAEKLVGSLEAERRHDKVAALRFPKPAPCGKTPLRAFELSKS
                     YGSLEIFTDVDLAVDKGSRVVVLGLNGAGKTTLLRLLAGVEEPDTGHVDPGHGLKLGY
                     YAQEHETLDTGATVLENMRRGAPDLTDTEVRSVLGSFQFSGDDALKPAKVLSGGEKTR
                     LALAMLVVSQANVLLLDEPTNNLDPASREEILGALRTYEGAVVLVTHDEGAVDALEPE
                     RVVLLPDAVEDLWGRGYRDLVSLA"
BASE COUNT         1055 a         2413 c         2316 g         1061 t
ORIGIN      
        1 ggagcaggcc cgcgacgacg aacgcgatgc tagcggcgag ccattgcggg gcgccgagca
       61 ggatcgcgaa tgcgcccgcg agggcaccgc ccgcgcacat caagagcgtc aggtccaggg
      121 tgaagatctc cgcgatgccc agaagcaacg cgccaaggaa ccaccacatg gactgcatgt
      181 ccccacctcc ggaagatgaa cttcgcttgc ttgccatagt agggcgaagg ggccgcgggc
      241 gccgtccacc tacggaatga ttgccttctt gcgcgccgtc catcttccgg atgattcgtt
      301 cagttccacc gggattccgt acgcgagtga gaggttcgcc tccgtgatgg tggccgagag
      361 cggccccgcg gcaaccaccc tgccgccccc cagcaggagc gcgtgcgtga agccccgcgg
      421 aatctcctcg acgtggtggg taaccaggat gaggacgggc gagcgcggat cggcggcgag
      481 ttccgtcagc gcctcgagca gttcctcgcg accggtgagg tcgagcccgg ccgcgggctc
      541 gtccaggagg aggatctcgg gatccgtcat cagcgcccgg gcgagttgaa cgcgcttgcg
      601 ctcgccctcg ctcagcgtcc ggtactccct gcccccgaag tcctggatcc caaacgcggc
      661 catgagggcg cccgctcgct cgaagtcgac gaggtcgtag ggctccttcc accgacccgt
      721 gacgccgtgg gcggcggtca tgacgacgtc cctggcgagt tcgcgtcccg gaatccgcgc
      781 ggcgacggcg tcgctcgcga ggccgaccct cagtccgagc tcctggaccg cggtggtccc
      841 gagtcgctct ccgaggaccc acaccgcgcc ctccgagggg tgggccctgg ccgcggccag
      901 cgtgaggagc gaggtctttc ccgccccgtt ggggccgagg atcacccagc gctcgccctc
      961 gttcacggtc caatcgaccg cggtgaggac ggcgttgccc tcccgccgca acgtcgcacg
     1021 ctcgacgcgc aggacttccg tcatggcttc gagcctagcg tcacgctagg agcgcctcgt
     1081 agacggccac ggttcgatcc gcgatcgcct cccaggagaa gtgctcggcg gccctcacct
     1141 tgcccgccgc gcccatgacc ttcgcgcgcg cggggtccgt cgcgaccgtc tccagcgccg
     1201 aggcgagatc cgcgacgaag cgctcggggt caatgggcgt acccgtgccg tcgttcacct
     1261 ggtcgatcgg aacgaggacg cccgtctcgc catcgacgac gacctcgggg attcccccgg
     1321 tcgcggtacc gacgacgggc agcccaacgg ccatcgcttc gaggttgacg atgccgagcg
     1381 gctcgtacac cgaggggcac acgaacgcgg tgcaggcgtc gagcacggcg acgagctcgc
     1441 gccgagggag tatcttctcg atccagatca ccccactgcg cgtctcctgg agagtggcga
     1501 ccgcgcccga gacctcggcc gcgatctccg gggtgtccgg cgcgcccgcg cacaacacca
     1561 cctgaatctc gggcgagagg cgcgcgacgg cctgcaggaa gtagggaaga cccttctggc
     1621 gcgtgattcg cccgacgaag acgaccgccg gccgatcggg atcaatcccg tagccgcgca
     1681 ccagggcctc ggcttcgacc cgctcgtctc cgacgggagc gctccagccc tcgacgtcga
     1741 tgccgttgtg tacgacgccc acccgcgtcg gatccatgaa cgggtagacc cgcagcacgt
     1801 cggcgcgcat gccctcgctc acggcgatca cgcccgcggc ggcctcgtag gcggtgcgct
     1861 cgatccatga cgagacccgg tagccgccgc cgagttgctc ggccttccac ggccgaaggg
     1921 gctcaagcga gtgggcggtg agcacgtggg gaacgtcgtg aagcaaggag gcgaggtggc
     1981 cggccatgtt tccgtaccag gtgtgcgaat gcacgaggtc cgcgccccgg cagtcgcgcg
     2041 ccatagggat tccgagggcg aggacgtcaa gcgcggcgtc ccccgtggag gtgccgagga
     2101 cgtagccctc gacccccggc tcgtcccggg gtccgtcgaa gcaccgcacg cgcacgtcga
     2161 gccttggccg gaggacgcgc gcgagttcgg cgacatggac gcccgcgccg ccatagacgt
     2221 gcggggggta ctcacgggta aggatgtcga cgcgcacggt cccacgctat cccgccccgg
     2281 gccacttcct caccccgaag cgcctcctgt ccgcccgtgg ccctctcccg atagactgcc
     2341 gccatgccca actcttccgt tctcgcgatc gttctcgccg ggggcgaggg caagaggctc
     2401 atgccgctca cgaacagccg atgcaagccc gcggtgccct ttgggggcac ttttcgcctc
     2461 gtcgacttcg cgctctccaa cctcatcaac tcgcgctatc tccacgtcgt ggtgctgacg
     2521 caatacaagt cacactccct cgaccgccac atcgcgcgca cgtggaggat gtccgccctc
     2581 ctcgggaact acgtcgcccc cgttcccgcc cagcagcggc ggggaaagca ctggtacctc
     2641 ggcagcgccg acgcgatcta ccagtgccag aacatcattg acgacgagag gcccgacatc
     2701 gtgatcattg tcggggccga ccacgtctat cgcatggact tctcgcagat ggtcgacgcc
     2761 cacgtggcgt ccggggcgga attcacggtc gcgggcattc gacagcccat cgagatggcg
     2821 tcagagttcg gcgtcatcga cgtcgacccg aagaaccccg gacacatcag ggccttcctc
     2881 gagaagccga agaacccgca gggccttccc gacagcccgg gcgagattct tgcgtccatg
     2941 ggcaactacg tcgcgtcgac ggacgcgctc ctcgcggcgc tcgctgccga cggcgccgac
     3001 gagtcgagcg cccacgacat gggggggaac atcgtccccc acttcgtcga gaggggcgcc
     3061 tgcggcttct acgacttccg cgacaacgtc gtccccggct ccacggacag ggaccgcgcc
     3121 tactggcgcg acgtcgggac gctcgacgcg tacttcgagg ccaacatgga cctcatcgcc
     3181 ctcgagccca tcttcaacgt ctacaacaag aagtggccgg ttcaccaggg gctcatcacg
     3241 caaccgcccg ccaagttcgt gcactcggag gagggtcggc tcggccacgc cgccgactcc
     3301 gtcgtctccc ccggcgtcat cgtctccggg gcgaccgtca ccggctccgt gctatccccc
     3361 gacgtccgcc tccattcgtg gtcgtcggtc tcggactccg tgctcctgga tcacgtcatc
     3421 gtcaaccgcc acgcgcaggt gcgcagggcg atcctcgaca agaacgtcgt gcttgaggag
     3481 ggcgccgcgg tgggactcga ccgcgagcac gacatcgagc gcgggttcac cgtgacggac
     3541 agcggcatca ccgtggtggg caagggcgtc ttcgtccccg cgtgaccggg cgcctcgccg
     3601 cgctcgtcgc tcgcggcgcc cgttactcgg tgcgggtgtc actcagtgcg gccgacgacc
     3661 cccacgagcc gcgcggatct cgcggcgagc cccggccact cgccgtcaaa ctccaggacc
     3721 acggccatcc ccgtcggaag cccgccggaa acacggtcga cggcctcgcg agacgaaccc
     3781 ggccccgcca ggaacagtgc ggccgcgctg atcgtcggct cgtgccccac gacgatgacg
     3841 ttcgtcgcgc ccggctcggt tcccgcaagc aaacccacga gcgtcgccac cgtggtggca
     3901 tacagctcgc gccgaaggtg gaccacggga acctcccacg ccgtggacgc gagttcccac
     3961 gtctggctcg tccgcacgga gtccgagacg taggcgacgt ccggctcgaa gcccgccgcc
     4021 gcgagcgcgg cgccgagttc cttggcgtcc gtgcgtccgc ggagggcgag ggtgcgctcg
     4081 tggtcggtga gcgcgcccgt gtgcgcctca gccttggcgt gacgggcgag caccaggacg
     4141 cgcatggttc ctactgtccc atcgcgtgga ccccgccgtc cacgtggacg accgagcccg
     4201 ttgtcgcggg aaaccagtcg gagaggagcg ccgttacggc gcgcgcggtc ggctccggat
     4261 cgctcgaatc ccatccgagc ggcgcgcgct cgtcccactt gctctccatc tggtcgaagc
     4321 ccggaatgtg cttggcggcc gtcgtcatga tggggccggc ggcgacgagg ttgcagcgga
     4381 ttccctcggg gccgaggtcg cgggccacgt agcgggacgt ggcctcgaat gcggccttgg
     4441 cgacgcccat ccagtcgtac accggccagg cgtagcggcc atcgaaggtc aggccgacga
     4501 tggagccgcc tcccgtcatg agcggccgag tagcggccgc gagggccttc agggagtagg
     4561 cggagatgtg gatcgcggtc gcgacgtcct cccacttccc gctcatgaag ttgcctccca
     4621 tgacggactg aggggcgaac ccgatggagt gcaccactcc gtccaggtgc gggacgtgct
     4681 cgcgcacccg gtgcgcgagg gcggacagtt ggtgctcgtt cgtgatgtcg agctcgacga
     4741 ccggagcctc gacgggcaga cgcccgctca tgctctgggt gaccttcatg gtgcgcccga
     4801 cgcccgtcag gacgaccgtg gcgccctgct cctgacacag gcgcgcgacg gagaaggcga
     4861 tcgagccctc cgtcagcact ccggttatga ggatgttctt gccttcgagg aatcccattc
     4921 ccagccctcc tggttcaatg tctacgccgc cttgccggtg ccagagcgct catgcccgcg
     4981 cgctagtgcc cgagcccgag cccgccgtcg acgggaatga ccgcgccgga gatgtacgcc
     5041 gcgtcgtcgg aggcgaggaa gaacgccgcc cccgccacct cgtcggcctc cccgaagcgc
     5101 cgcgccggaa tcgagtcgcg gtactgcttc tgtacgtcat cggtcaggac cgcggtcatg
     5161 gccgtgttga tgaatcctgg cgcgatgacg ttcgcggtga tcccgcgcga gccgatctcg
     5221 cgggtgatgg atcgcgccat gcccacgagc gccgccttcg acgacgagta gttgacctgg
     5281 ccggggtttc ccatgaggcc gacgaccgaa ccgatgagga tgatgcgtcc ggacttcttc
     5341 ttcatcatcg acgtggacgc ccggcgtacg caccggaagg ccccctgaag attgacgccg
     5401 atcacggcgt cgaaatcctc gtccgtcata cgcatgagta gcccatcctt ggtgatgccc
     5461 gcgttcgcga cgaggacatc gatgggcccg tgcttcgcct cgaaggcgtc catggcggcg
     5521 ttgaccgcct cggagtcgcg aacgtccccc acgatcccgt caatggggtc gggaggagcg
     5581 tctccgatgt agatcgtgct gaccgtgtca cccgccgcga ggaagcgttg ggcgatggcg
     5641 agaccgatgc cgcggttcgc ccccgtaatg tgtacgtgtc gtgccatggt gacgacggta
     5701 gcggacaatc gccaaagggg gcaccagcga ctcgttcgcg ggctcgcgcc cccggacggc
     5761 cctgcggcta cgcgagggag acgaggtcgc ggtaaccgcg accccagagg tcctcgacgg
     5821 cgtcgggaag cagcacgacg cgctcaggct cgagcgcgtc gacggcgccc tcgtcgtggg
     5881 tcaccaggac gaccgcgccc tcgtaggtgc gcagcgcgcc gaggatctcc tcgcggctcg
     5941 cggggtcgag gttgttggtt ggctcgtcga gtagcaggac gtttgcctgg ctcaccacga
     6001 gcatcgcgag ggcgaggcgc gtcttctcgc cgccggagag caccttcgcg ggtttgaggg
     6061 cgtcgtcgcc cgagaactgg aaggacccga gcacgctgcg cacctcggtg tcggtcaggt
     6121 cgggcgcgcc ccggcgcatg ttctccagca ccgtcgcgcc cgtgtcgagg gtctcgtgct
     6181 cctgggcgta gtagcccagc ttgagcccgt gacccgggtc gacgtgcccg gtgtctggct
     6241 cctcgacccc cgcgaggagc cgcaggagcg tcgtcttgcc cgcgccgttg aggccgagga
     6301 cgacgacgcg ggagcccttg tcgacggcaa ggtcgacgtc ggtgaagatc tcaagcgagc
     6361 cgtaggactt gctgagctcg aaggcgcgca acggggtctt cccgcacggc gccggcttgg
     6421 ggaaccggag cgcggcgacc ttgtcgtggc gacgctccgc ctccagcgat cccacgagct
     6481 tctcggccct gcgaagcatc ccctgggcgg cgacggcctt ggtcgccttc gcgcgcatct
     6541 tgttcgcctg ggcgatgagc gcctcggcct tgcgctcggc gttgtcgcgc tcgcggtgac
     6601 gacgcttctc gtcctcctcc cgctgctgga ggtaggcgtc ccacccgagg ctgtactggt
     6661 cgagttccga gcggttggcg tcgaggtgga acaccttgtt ggcgcacgcg cgcaggaggc
     6721 cggcatcgtg cgagatcacg acgagtcccc cggagtacga gcggaggaag tcgcggagcc
     6781 agacgatgga atcggcgtcg aggtggttgg tcggctcgtc gaggagcagg gtctccgagt
     6841 ccccg
//
LOCUS       DASVKP010000048         1971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_507152, whole genome shotgun sequence.
ACCESSION   DASVKP010000048 DASVKP010000000
VERSION     DASVKP010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507152"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VF362_01010"
     CDS             complement(<1..280)
                     /locus_tag="VF362_01010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006215708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7589539.1"
                     /translation="MKTLVIIPTYNERESLPVQLAGVRRFAPDVDVLVVDDNSPDGTG
                     EWADAQAVADAHVSVLHRPGKAGLGAAYRAGFAWGLERDYEILVEMDAD"
     gene            complement(280..1872)
                     /gene="lnt"
                     /locus_tag="VF362_01015"
     CDS             complement(280..1872)
                     /gene="lnt"
                     /locus_tag="VF362_01015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX7589540.1"
                     /translation="MIRRRTTRAQRLRASRRGRGALALRNIVVAAIAGWAINLAFPTA
                     NLWVAAPIGLALLWSALEHSGVRGGFGLGMVAGLGFFLPHLWFIDVSAGIVPWLALAT
                     FESLAIALPTAAWCWVRTRKVLDGRVWAEPLVFGLLWVAGEQLRSIAPYGGFPWGRLA
                     FSQIDAPVAALAWLGGAPLVSFAVATAGGALGLAFEAIRGRRVVLSALAIGGAFAVYA
                     AALAIPLDVAPESGTLRVGLVQGNVPNLGLDSFRQARLVTQNHADETDRLVADLPWAP
                     DVVVWAEDSADVDPRVDATSHRIVTDAAVAVGSPILLGTNDYSPVNGRYNTALLWSPE
                     GEAIAFYNKQHPVPFAEYLPLRTLARAVTEVADRLTTDMLPGDRVGLVSLPSERLGRD
                     VPLGDVICFEVAYDPLIRSSVRAGAEIVVVQTNNATFGLTNESEQQFAMTRMRAIETG
                     RVAIQVSTVGVSGFVLPDGSVVSKTGLFTAEHLAEEVPLRTSMTPAMRVGGVLGFCFL
                     IAPVGLWLIRWLMSLRGRWDWD"
BASE COUNT          344 a          705 c          669 g          253 t
ORIGIN      
        1 cgtccgcgtc catctcgacg aggatctcgt agtcgcgctc cagcccccac gcgaagcccg
       61 cgcggtaggc cgcgccgagc ccggccttgc ctgggcgatg caggacgctg acgtgggcgt
      121 cggccacggc ctgagcgtcc gcccactccc ccgtcccgtc gggcgagttg tcgtcgacga
      181 cgagcacgtc tacgtcgggg gcaaacctgc gcacgcccgc gagctggacg ggaagcgatt
      241 ccctctcgtt gtaggtcggg atgatcacga gggtcttcat cagtcccagt cccatctgcc
      301 gcgcagcgac atcaaccacc ggatcagcca caggccgacc ggggctatca agaagcagaa
      361 tccgagcact cccccgaccc gcatggccgg ggtcatcgag gtgcggagcg gcacctcctc
      421 ggcaaggtgt tcggcggtga acagccccgt cttcgacacg accgagccat cgggaaggac
      481 gaatccgctc acccccaccg tcgagacctg aatggccacg cgccccgtct cgatcgccct
      541 catccgggtc atcgcgaact gctgctcgga ctcgttggtc agcccgaacg tcgcgttgtt
      601 ggtctgcacc acgacaatct cggcgcccgc gcggacggat gaccgtatca gggggtcgta
      661 ggcgacctcg aagcagatga catcgcccag cggcacgtcg cgcccgagcc gctccgacgg
      721 cagcgacacc aggcccacgc ggtccccggg gagcatgtct gtggtcaggc gatccgcgac
      781 ctccgtcacg gcccgcgcga gcgtgcgcag gggcaggtac tccgcaaacg gcacgggatg
      841 ttgcttgttg tagaaggcga tcgcctcgcc ctcgggggac cacaggagcg cggtgttgta
      901 ccgcccattg acgggcgagt agtcgttcgt tcccagcagg atcggggagc cgaccgccac
      961 ggccgcgtcc gtgacgattc ggtgtgaggt cgcgtcgacg cgggggtcga cgtcggccga
     1021 gtcctccgcc cacacgacga cgtcgggagc ccagggaaga tccgcaacga ggcgatccgt
     1081 ctcgtccgca tggttttggg tcaccaggcg cgcctggcgg aacgagtcga gaccgaggtt
     1141 agggacgttg ccctggacca ggccgacgcg cagcgttccc gattccggcg cgacgtccag
     1201 ggggatggcg agggccgccg catacacggc gaaggctccg ccgatcgcga gggccgagag
     1261 aacgaccctc cgtccgcgaa tcgcctcgaa cgcgaggccg agggcgccgc ccgccgtcgc
     1321 gaccgcgaac gagaccaatg gcgctccccc gagccacgcc agggccgcca cgggggcgtc
     1381 aatctgggag aaggcgagcc ttccccacgg aaagcccccg tacggggcga tggaccggag
     1441 ctgctccccc gcgacccaga gaaggccgaa cacgaggggc tcggcccaca ccctgccgtc
     1501 cagaaccttc ctcgtcctga cccagcacca cgcggccgtc ggcagggcaa tggcgagcga
     1561 ctcgaacgtc gcgagggcga gccagggcac gatccccgcc gagacgtcga tgaaccagag
     1621 gtgaggaagg aagaagccga gcccggcgac catccccagg ccgaaccccc cgcgcacgcc
     1681 cgaatgctca agcgcagacc ataggagcgc gaggccgatg ggcgcggcga cccagaggtt
     1741 cgccgtgggg aacgccaggt tgatggccca cccggcaatc gccgcgacca cgatgttccg
     1801 cagcgcgagc gcgcccctgc cgcgccggga cgcgcgcagg cgttgcgccc tggtcgtcct
     1861 tcgccgaatc acgcgcccag cctacgcgag cgacgggcgg gcgaacctcg ccctaggacg
     1921 tgttgaataa gtcggtgttg acccagatca ggatcgcggc gacggtcacg c
//
LOCUS       DASVKP010000049         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1165429, whole genome shotgun sequence.
ACCESSION   DASVKP010000049 DASVKP010000000
VERSION     DASVKP010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165429"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..723
                     /gene="ruvA"
                     /locus_tag="VF362_01020"
     CDS             133..723
                     /gene="ruvA"
                     /locus_tag="VF362_01020"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX7589541.1"
                     /translation="MIAQLAGTVLAVDASSLVIDVGGIGLHVLSTPGTLAGARAGQAI
                     RVWTHLVVREESLTLFGFASKEERDTFVALQGVSGVGPRLALAMLAVHAPRTLAAAVA
                     TGDRGALERVPGIGAKVAARLLLELGGKLVLPDADGVVAAGDGRDQVVEALIGLGWNA
                     KGARAAVDAVARGPIEDSEVPTVLRAALQRLGGTHG"
     gene            716..1849
                     /gene="ruvB"
                     /locus_tag="VF362_01025"
     CDS             716..1849
                     /gene="ruvB"
                     /locus_tag="VF362_01025"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX7589542.1"
                     /translation="MDEPLAESDLARVVALERVADLERVVDLERVAGLERVVDLDADA
                     IERSNEAALRPSSLDEFVGQRVVRDQLGLVLKAAKGRGAPADHALLSGPPGLGKTTLA
                     MIVAGEMGATLRVTSGPAIQHAGDLAAILSSLAEGEVLFIDEIHRLARPAEEMLYLAM
                     EDFRVDVVVGKGAGASSIPLSLPRFTVVGATTRAGLLPAPLRDRFGFTAHLDFYSDDE
                     LRRIIERAAGLLGFPVDADAASEIAGRSRGTPRVANRLLRRVRDWAEVHGDGRGSLGA
                     AREALAVYEVDELGLDRLDRAVLGALCTRFGGGPVGLKTLAVAVGEETETVETVAEPF
                     LVREGLMSRTPRGRIATPAAWEHLGLDVPREGIVGFGGSRLFG"
     gene            1946..>2243
                     /locus_tag="VF362_01030"
     CDS             1946..>2243
                     /locus_tag="VF362_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX7589543.1"
                     /translation="MSTVTKAARNSLLWLLTIIVALGGGIVYEALTQPRTPDQSPWLP
                     RLGLDLAGGRIIILQAKTIDGTAVTTDSLKQSVEIIRRRVDASGVAEALITTENP"
BASE COUNT          302 a          797 c          821 g          323 t
ORIGIN      
        1 ggtgaggagg gcatcgcgac cccggcccag gcccgatggg ccgaggcgga gcgtgcggcg
       61 cgtcgcaccg gaaggaagcg ccccttgccg tagcgttgtt cgcacatacg ttcgagccgc
      121 gcgggaggat cgttgatcgc acaacttgcg gggaccgttc tcgcggtgga cgcgtcgtcc
      181 ctcgttatcg acgtcggcgg catcgggtta cacgtgctct ccacgccggg caccctggcg
      241 ggcgcccggg cgggacaggc gatccgggtg tggacgcacc tcgtcgtgcg cgaggagagc
      301 ctgacgctgt tcgggttcgc gtccaaggag gagcgggata ccttcgtcgc cctccagggg
      361 gtgagcgggg tcgggccccg tctcgcgctc gcgatgctcg ccgtccacgc gccccgcacc
      421 ctcgcggccg ccgtcgcgac gggggacagg ggcgccctcg agcgcgtccc cgggatcggc
      481 gcgaaggtcg ccgcgcgact tctgcttgag ctcggcggca aactcgtgct ccccgacgcc
      541 gacggcgtcg tcgccgcggg cgacgggcgc gaccaggtgg tcgaggcgct catcgggctc
      601 ggatggaacg ccaagggcgc ccgcgcggcg gtcgacgcgg tcgcccgcgg ccccatcgag
      661 gactccgagg tgccgaccgt ccttcgggcg gcgctgcagc ggcttggagg aactcatgga
      721 tgagccctta gccgagagcg atctggcgcg cgtcgtcgcc ctggaacgcg tcgctgatct
      781 tgaacgcgtc gtcgacctgg aacgcgtcgc tggtcttgaa cgcgtcgtcg acctggacgc
      841 ggacgcgatc gagcgctcca acgaggccgc gctccgcccg tcgtcgctcg acgagttcgt
      901 gggccagcgc gtcgtgcgcg accaactcgg gctcgtcctc aaggcggcga agggccgcgg
      961 cgcccctgcg gaccacgccc tgctgtccgg cccccccggg ctcggcaaga cgaccctcgc
     1021 gatgatcgtc gcgggagaga tgggcgcgac gctccgcgtc acctccgggc ccgcgatcca
     1081 gcacgccggc gacctcgcgg cgatcctttc ctcgctcgcc gagggcgagg ttctcttcat
     1141 cgacgagatc caccgcctcg cgcggccggc cgaggagatg ctttacctcg ccatggagga
     1201 cttcagggtg gacgtggtcg tcggcaaggg ggcgggggcc tcctcgatcc cgctcagcct
     1261 tccccggttc accgtcgtcg gggcgacgac gcgggcaggg ctgttgcccg cccccctgcg
     1321 cgatcggttc ggcttcacgg cccacctcga cttctactcc gacgacgagc tccgccgcat
     1381 cattgagcgc gcggcgggcc tcctcgggtt ccccgtggac gccgacgcgg cgagcgagat
     1441 cgccgggcgt tcccggggca ccccgcgcgt cgccaaccga ctcctgaggc gcgtacgcga
     1501 ctgggccgag gttcacggcg acggcagggg aagcctcggc gccgcccgcg aggccctcgc
     1561 ggtgtacgag gtggacgaac tcggactgga tcggctcgac cgcgccgtcc tcggcgcgct
     1621 gtgcacccgg ttcggcggcg ggcccgtggg cctgaagacc ctcgccgtcg cggtggggga
     1681 ggagacggag accgtcgaga cggtggcgga gcccttcctg gtgagggagg ggctgatgag
     1741 caggacgccg cgcggtcgaa tcgcgacccc cgcggcgtgg gagcacctgg gactcgacgt
     1801 tccccgggag ggcattgtgg gcttcggcgg gtcgcgcctc ttcggctaat tggcccctcg
     1861 cgggcgggcc ggttcgacgc gggtcgcggg aaccgcctag aatcggctgg gctcccccga
     1921 gccgctcccg agccgaagga caaccgtgtc gaccgtcacc aaagccgcgc gcaactcgct
     1981 cctgtggcta cttacgatca tcgtcgccct cgggggtggg atcgtgtacg aagccctcac
     2041 gcaaccgcgc acgccggacc agagcccatg gctcccgagg ctcggcctcg acctcgcagg
     2101 cggcaggatc atcatcctgc aggcgaagac gattgacgga accgcggtga cgaccgacag
     2161 cctgaagcag tcggtcgaga tcattcgccg ccgcgtcgac gcctcgggtg tcgcagaggc
     2221 cctgatcacg acggagaacc cga
//
LOCUS       DASVKP010000050         4387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049 k141_95903,
            whole genome shotgun sequence.
ACCESSION   DASVKP010000050 DASVKP010000000
VERSION     DASVKP010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95903"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VF362_01035"
     CDS             complement(<1..703)
                     /locus_tag="VF362_01035"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX7589544.1"
                     /translation="MTSSERPERSTTRPVRIPRRRSPWVPIVVALAGVMVVAVVLAQV
                     WTEVAWYRELGFGQVWRAEWSAKAVLFVVGGVLAFAAVWASLVIARRARPKVSAVQHS
                     VFESYREQIRPIERGATVVIPVVAGLISGLVLASNWRTVLAWRYHVPFGTVDAQFGHD
                     LSFYVFTLPALTLFLDFALAILIVCLALSAFVHLLFGAITTGGRTFAATGVARVQLAV
                     LGSAVMIVIAVKIWVS"
     gene            complement(756..1856)
                     /locus_tag="VF362_01040"
     CDS             complement(756..1856)
                     /locus_tag="VF362_01040"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX7589545.1"
                     /translation="MRGNDVGDASPELLAQPLGQPPRTPGIRSIVMALSGMVSALLIA
                     VLVVLPAPFMIESAGPTFDVFDGKVEGTTLITIDGATTYPSSGQLRLTTVAVSRTSGT
                     LFSLGPVVRAWLNPASATYPDVPIDPNYDAVVQQEWTSSQENATVAALGELGTAVPAR
                     IRIADFSPGSNAEGKLMVDDIIVSVDGKQILTLPDLSAAIGALAPGDPVTVVVSRAGK
                     DVTATFATISGQNGKTLMGVAIDPTFDMPYTVNVAIDHVGGPSAGLIFTLAIVDLLTP
                     QDELQGAKVAGTGTISPNGDVGPIGGIALKMIGARRAGVDWFLAPESNCSEVVGHVPA
                     GLRVVSVANLSEAYDAIVAIGSHAADNLPTCS"
     gene            1958..3295
                     /locus_tag="VF362_01045"
     CDS             1958..3295
                     /locus_tag="VF362_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX7589546.1"
                     /translation="MSDDSSEWMKLLRELFGDRAEEALDEMRRMGVDPAQLAAAIGVD
                     ATPGMLDHMLSQLRDLMAQSAGEDVNWTLAHDVARGVAAQGGDPMVSAAAAADHRAAV
                     STAELWLDAATDFAPSTLVPRIWSHAEWVEATLPTWRTLAGPVAVSVSRALGALLSHE
                     SDAGGALLGQISPTVCGMHVGQAAGAMAREAFGGTDLGLPLLPEPQVALVPRSISEFA
                     EGLDVPADEVRSFLALRECAHVRLFKHSPWLQGQLHVAIERYASGVSIDLDALDEAVH
                     QAGMGDPDTLQKALSKGIFATAHTDDQRTMLEAIETMLALIEGWVDDVTAQAAAPHLR
                     HLGQLREMMRRRRAAGGPAEHTFATLLGLELRPRRLRDAVDLWTALRVAGGTSGRDDP
                     WAHPDLLPGPGDLADWRGWVAARGAVGSDDDVDRELREMLGEGSDGPSGTVSD"
     gene            3450..3578
                     /locus_tag="VF362_01050"
     CDS             3450..3578
                     /locus_tag="VF362_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEX7589547.1"
                     /translation="MKDKAKVQATGDISVTNGRRMAKGICPNCGTKLTRILPKAQA"
     gene            3715..>4387
                     /locus_tag="VF362_01055"
     CDS             3715..>4387
                     /locus_tag="VF362_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589548.1"
                     /translation="MRLRPGVPVLHLDEGLIQIGLRDPLLLHGLCRAEAHFLASLEGR
                     SVPVSSSERRDFPESVRALERQPHLLWRDDPAQGRLASAMVRWRGCTLPALEAARILA
                     LAGVRTMSAVDPRPVSPADPYSPSLRGLARAEALARAVAETGAEVRWASRESPADFEV
                     LSAHGAPDLVSTRELLARDVAHLLIVSDEDGLTVGPVVVPGVTACAGCLAHARADRDP
                     RWPRIA"
BASE COUNT          684 a         1530 c         1510 g          663 t
ORIGIN      
        1 ggctcaccca aatcttgacg gcgatcacga tcatcacggc cgagccgagg accgcgagtt
       61 ggacccgcgc gacgcccgtc gccgcgaagg tccgcccgcc cgtggtgatc gccccgaaca
      121 gcaggtggac gaacgccgag agcgcgaggc agacgatgag gatggcgagc gcgaagtcga
      181 ggaagagtgt gagcgccggg agcgtgaaca cgtagaacga aaggtcgtgg ccgaactgcg
      241 cgtcaacggt cccgaaggga acatggtatc gccaggcgag gaccgtgcgc cagttcgacg
      301 ctaggaccag gccggagatg aggcccgcga caacggggat caccacggtc gcgccgcgct
      361 cgatcggtcg gatctgttcc cgatacgact cgaatacgct gtgctggacc gcgctgacct
      421 tgggccgcgc gcggcgagcg atcacgaggc tcgcccacac cgccgcgaac gccaggacgc
      481 ccccgacaac gaagaggacc gccttggcgg accactccgc cctccacacc tgaccaaagc
      541 ccaactcgcg gtaccacgcg acctcggtcc acacctgcgc gagcacgacc gccaccacca
      601 taaccccagc gagtgccacg acgatgggaa cccagggcga gcgcctgcgc gggatgcgga
      661 cgggtcgggt ggtgcttcgt tcgggtcgtt cgctgctcgt cacgtgctat cgtcctagag
      721 tttcgaggga tgtcgcctga actagccttg catactcacg agcaggtcgg caggttgtcc
      781 gccgcgtggc tcccgatcgc gacgatggcg tcgtaggcct cggagaggtt cgctaccgag
      841 acaacgcgaa gcccggcagg aacgtgccca accacctccg agcagttgga ctcgggcgca
      901 aggaaccagt cgacccctgc tcgccgcgcg ccgatcatct tgagggcgat gcctccgatg
      961 gggccgacgt cgccgttggg tgagatggtc cccgtgcccg cgaccttggc cccctggagt
     1021 tcgtcctggg gggtcaggag gtcgacgatc gcgagagtga agatcaggcc agcgctcggg
     1081 cccccgacgt ggtcaatcgc cacgttcacc gtgtacggca tgtcaaacgt cggatcgatc
     1141 gcgaccccca tcagcgtttt gccgttctgg ccgctgatgg tggcgaaggt ggccgtgacg
     1201 tccttgcccg cccgcgagac gaccacggta accggatccc ccggcgcgag cgcgccgatc
     1261 gcggcggaca ggtcgggcag cgtcaggatc tgctttccgt cgaccgaaac gatgatgtca
     1321 tcgaccatga gtttgccctc ggcgttcgat ccgggggaga agtcggcgat gcggatcctc
     1381 gcgggcacgg cggtgcccaa ctcccccagc gcggccaccg tcgcattctc ctgcgaactc
     1441 gtccactcct gctggaccac cgcgtcgtag ttcgggtcga tcgggacgtc cgggtacgtg
     1501 gccgatgccg gattgagcca ggctcggacg accgggccga gcgaaaagag cgtccccgac
     1561 gtgcgggaca cggcgaccgt cgtgaggcgg agctgtccgg acgaagggta ggtggttgcg
     1621 ccgtcgatcg tgatgagcgt cgttccctcg accttgccat cgaacacgtc gaaggttggc
     1681 cccgcgctct cgatcatgaa gggtgcgggg agcaccacga ggacggcgat caggagtgcc
     1741 gaaaccattc cgctcagcgc catgacgatg gaccggatgc ccggggtacg cggcggctgg
     1801 ccgagcggtt gggccagcaa ttcgggcgaa gcgtcaccca cgtcgttccc tctcatgcga
     1861 ccatcatcgc tgatggcgaa ctccgccccg accaagtccc atcccggcgt accgtggtgg
     1921 cgacgtgtgt gctgtgtacc gttcccaggg aggcgaagtg agcgacgact cgtcggagtg
     1981 gatgaagttg ttgcgcgaac tcttcggcga ccgcgcggag gaggctctcg acgagatgcg
     2041 gcgcatgggg gtcgacccgg cccaactcgc cgccgcgatc ggcgtggacg cgaccccggg
     2101 catgctcgat cacatgctgt cgcagttgcg cgaccttatg gcgcagtccg ctggcgagga
     2161 cgtgaactgg accctcgcgc acgatgtcgc gcgcggcgtc gcggcgcagg gtggcgaccc
     2221 gatggtgagc gcggcggcgg ccgccgatca ccgtgcggcc gtctcgacgg ccgagttgtg
     2281 gctggacgcc gccacggact tcgctccttc gacgctcgtc ccccggatct ggagccacgc
     2341 cgagtgggtc gaggcgacgc ttccgacatg gcgaactctc gccggccccg tcgccgtctc
     2401 cgtgtcaagg gcgctcgggg cgctcctttc acacgagagt gacgcggggg gggcgctgct
     2461 cggacagatc agcccgaccg tctgcggaat gcacgtcggc caggctgcgg gcgccatggc
     2521 gcgggaggcc ttcgggggca ccgaccttgg cctcccactc ctgcccgaac cccaggtcgc
     2581 gctcgtgcca cgctccatct ccgagtttgc cgagggactc gacgttcccg cagacgaggt
     2641 ccgttcgttc cttgcgctcc gcgagtgcgc ccacgtccgg ctcttcaagc actcgccgtg
     2701 gctccagggc cagcttcacg tcgccatcga gcgctatgcg agcggcgtca gcatcgatct
     2761 cgatgccctc gacgaggccg tgcaccaggc cgggatgggc gatcccgaca cgctccagaa
     2821 ggctctgagc aaggggatct tcgcgacggc ccacacggac gaccagcgca ccatgctcga
     2881 ggcgatcgag accatgctcg ccctgattga gggatgggtc gatgacgtca ccgcccaggc
     2941 ggcggccccg cacctgcgcc acctcggcca actgcgcgag atgatgcggc gcaggcgcgc
     3001 cgcggggggt ccggccgaac acacgttcgc cactctgctc ggactcgagc ttcgcccgcg
     3061 ccgccttcgc gacgccgtgg acctgtggac ggcactccgg gtcgctggcg gaacgtcggg
     3121 acgggacgac ccgtgggccc accccgatct gcttccgggt cccggtgacc tcgccgactg
     3181 gcgcggctgg gtggccgcgc gcggcgccgt tggttcggac gacgatgtcg atcgagagct
     3241 tcgtgagatg ctcggcgagg ggtccgacgg tccttccggc acggtttccg attagggcga
     3301 cacgggcgca ccacctcacc cggattggcc gtgtcgggaa ctacgctgaa cggaagacac
     3361 aatagggggc aaagggcgcg ggcgctccag cccgccgaca cctgatcgga gcagtcatgg
     3421 cagacaagta cacgggcgag gtctagtgcc tgaaggacaa ggcgaaggtc caggccaccg
     3481 gtgacatctc cgtgaccaac gggcgccgca tggcgaaggg gatctgcccc aactgcggga
     3541 ccaagctgac ccgcattctc ccgaaggccc aggcctaagc gactcacagg ggggcgggcg
     3601 actcacgtcg cccgcccccc tgtgccgtct gcaacccgtg tgtcgtctgc accccgcgtg
     3661 cgcgcagcct gtggatatcg cccacgcccc ccgccgcgcg gccaaggtgg atccatgcga
     3721 ctacgaccgg gcgttcccgt cctccatctg gacgaaggct tgatccagat cggcctgcgc
     3781 gatccgttgc tcctccacgg cctctgccgc gcggaggcgc acttcctggc ctcgctcgag
     3841 gggcgctcgg tgcctgtcag ttcgagtgaa cggcgcgact tccctgagtc ggttcgcgcg
     3901 cttgagcgcc aacctcacct cctttggcgc gacgacccgg cgcaggggcg cctcgcgagc
     3961 gcgatggtcc gttggcgcgg ttgcaccctc cccgcgctcg aggcggcgag aatcctcgcg
     4021 ctcgcgggag tccgcacgat gtcggcggtc gacccgcggc cggtctcgcc cgccgatccc
     4081 tattccccct ccctccgcgg gctggcgcgc gcggaggcgc tggcgcgtgc cgtcgccgag
     4141 accggcgcgg aagtcaggtg ggcgtcccgc gagtcccccg ccgacttcga ggtgctctcg
     4201 gcgcacggcg cccccgacct cgtctccacg cgggaactcc tcgcacggga cgtggcgcac
     4261 ctcctcatcg tctcggacga ggacggcctg accgtggggc cggtggtcgt ccccggcgtg
     4321 acggcctgcg ccggttgtct ggcgcacgcc cgcgcggatc gcgacccgag gtggccgcgc
     4381 atcgccc
//
LOCUS       DASVKP010000051         4461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1248208, whole genome shotgun sequence.
ACCESSION   DASVKP010000051 DASVKP010000000
VERSION     DASVKP010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248208"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="VF362_01060"
     CDS             <1..1533
                     /locus_tag="VF362_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX7589549.1"
                     /translation="EIDGLPVAADGELLTGLLRDRWGFDGVVVGDYFGVAFLHLLHGI
                     AADLGEAAALALLAGIDVELPTGDAYVEPLKAALASGAVDVALVDRAVLRVLRQKEEL
                     GLLDETFEGPSPTGIDLDSPLHRALARRLAEESVILLANDGTLPLAAGAATSPSIAAI
                     GPNADRFAAMFGCYSFANHVLVHRPGVELGLEVPTLAAALEAEFPGRVTIARGCDVDS
                     ADESGLAEAVAAAAAADIAILAVGDHAGLFGRGTVGEGCDRDDLELPGLQRRLIDEVL
                     ATGTPVVLVLLTGRPYAVGWALERCAAALQVFFPGEEGAGAIAGVLSGRVNPSGHLPV
                     TMPRSAGAQPYSYLHPMLGGDSDVTNLSSVPARPFGHGLSYTRFDYSAFVAEDGAPTD
                     GSITAAVTVANTGDRRGATVVQLYGRVRHAPVTRPIAQLLGYLRLELDPGESRRVEFR
                     VPTTRLAFSDRDYRRVVHPGGIDLWVGNAATRVVEGEAMLSGPVHELREDSPRWTEAT
                     VS"
     gene            complement(1530..2102)
                     /locus_tag="VF362_01065"
     CDS             complement(1530..2102)
                     /locus_tag="VF362_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7589550.1"
                     /translation="MSRIVTVGPDQTMRLAEIGELTALAYLADGLVDAAHPYVPELRN
                     AKARAEHAILLMMADGDDGQGAAVGTITVVPSGSPFSEFNNEGEYELRMLAVSPLARG
                     RGIGEELALFGLETAMAQGATRVALSTMESMHAAHRLYEKLGFVRQPELDWVAHPSHI
                     KVRCDATCYDANGNCTEGGQKLLAYAWSPS"
     gene            complement(2220..4304)
                     /gene="ligA"
                     /locus_tag="VF362_01070"
     CDS             complement(2220..4304)
                     /gene="ligA"
                     /locus_tag="VF362_01070"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005507012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX7589551.1"
                     /translation="MNDPRARHAELIQAIEAHRAAYYERDAPTISDAEYDALERELRA
                     IEAEHPELADPDSPSQTVGGAVATGFASVRHRERMMSLDNAFSIADVAAWEERVERAG
                     GPREFVCEPKIDGLSISLTYEGGDLVRGVTRGDGTTGEDVTANVRTIAGLPSRLAGGG
                     VPEVVEIRGEVYLPVAAFEELNERLVEEGKAPYANPRNTAAGSLRQKDAAVTATRPLA
                     VTTYALGALEWGAAAPDPRAATQIGIYSVLKEWGLPVSELAEVHTGVGEVEAYLSRLE
                     ARRHALVHEIDGAVIKVNDRDAQRELGSTSRAPRWAIAYKFPPEEVNTRLEDIRVDVG
                     RTGRVTPYGVMTPVKVAGSTVTFATLHNAQEVARKGVLIGDTVVVRKAGDVIPEIVAP
                     VVAVRTGAERVWVMPTVCPSCGTALAQQKDGDVDLRCPNAEGCRGQIVDRIAFIGSRG
                     ALDVEVLGEKAAAALVDSGVLRNEAGLFDLTEDDLRCVPLLTLDQDSKKDGVARTAGD
                     VSTNGLRLLANLDAARSRPLWRVLVSLSIRHVGPTAARALATAFGSLDAIRGADPGAL
                     SSTDGVGPVIARSIAEWFGVPWHVEIVGRWAAAGVRMEDDGGAEGERVLAGLTIVVTG
                     SLEGFSREEAAEAIASRGGRASSSVSAKTNFVVIGANAGSKAAKAAEFGVPTLDEEGF
                     VVLLAGGPGAVA"
BASE COUNT          612 a         1677 c         1489 g          683 t
ORIGIN      
        1 gaaatcgacg ggctccccgt cgcggcggac ggcgagcttc tgaccggcct cctccgcgac
       61 cgctggggct tcgacggcgt cgtcgtcggc gactacttcg gcgtcgcgtt cctccacctt
      121 cttcacggca tcgccgccga cctcggcgag gcggccgcgc tggcgctcct tgcgggcatc
      181 gacgtcgagc ttcccacggg cgacgcctac gtggagcccc tcaaggccgc cctcgcgtcc
      241 ggcgcggttg acgtcgccct cgtggatcgc gccgtcctga gggtccttcg ccagaaggag
      301 gagctcggcc tcctcgatga gacgtttgag ggcccgtccc cgaccggcat cgacctcgac
      361 tctcccctcc accgcgcgct cgcgcgtcgc ctcgcggagg agtcggtcat tctcctcgcg
      421 aacgacggga ccctgccgct ggccgccgga gccgccacct ccccctcgat tgccgcgatc
      481 ggccccaacg ccgaccgatt cgccgcgatg ttcggctgct actcgttcgc gaaccacgtg
      541 ctggttcacc gccccggcgt cgagctcggg cttgaggtgc cgacgctcgc cgcggccctc
      601 gaagccgagt tccccggccg cgtcaccatc gcgcgagggt gcgatgtgga ctccgcggac
      661 gaatcgggtc tcgccgaggc cgtcgcggcg gcggcggcgg cagacatcgc gatccttgcg
      721 gtgggcgacc atgccgggct cttcgggcgc ggcaccgtcg gggaagggtg tgatcgcgac
      781 gaccttgagc tcccgggcct ccagcgcagg ctcatcgatg aggtcctcgc caccggaacc
      841 ccggtcgtcc tcgtcctgct caccggtcgc ccctacgcgg tcggatgggc cctcgaacgg
      901 tgcgcggcgg cactccaggt ctttttcccc ggcgaggagg gcgcgggcgc catcgctggc
      961 gtgctctcgg ggcgcgtcaa cccgtcgggc cacctaccgg tgaccatgcc gcgctccgca
     1021 ggggcccagc cctactcgta ccttcatccg atgctcgggg gcgactccga cgtcacgaac
     1081 ctttcgagcg tcccggctcg ccccttcgga cacggactct cgtacacgcg cttcgactac
     1141 tccgcattcg tcgccgagga cggtgccccc accgacggct cgatcaccgc ggccgtgacg
     1201 gtcgccaaca ccggcgatcg acgaggcgcg accgtcgtcc agttgtacgg acgcgtgcgt
     1261 cacgcaccgg tgacgcgacc catcgcgcaa ctgctcggct acctccggct ggaactggat
     1321 ccgggcgaga gcaggcgcgt ggagttccga gtgccgacca cgcgactcgc cttctccgat
     1381 cgcgactacc ggagggtcgt gcacccgggc ggcatagacc tctgggtggg aaacgcggcg
     1441 acccgagtgg tggagggtga agcaatgctc tctggtcccg tgcacgaact ccgcgaggac
     1501 tcgccgagat ggaccgaggc gacggtctcc taactcggcg accaggcgta ggccaggagt
     1561 ttctgcccgc cctccgtgca gttgccgttc gcgtcgtaac aggtggcgtc gcagcggacc
     1621 ttgatgtgcg aggggtgcgc gacccaatcg agttccggct ggcgcacgaa cccgagcttc
     1681 tcgtagagac ggtgcgcggc atgcatcgac tccatcgtgg agagcgccac ccgcgtcgca
     1741 ccctgagcca tcgccgtctc caaaccgaag agggcgagct cctcgccgat gccgcggccg
     1801 cgggccaggg gcgagacggc gagcatcctc aactcgtact ccccctcgtt gttgaactct
     1861 gagaagggcg acccgctcgg cacgaccgtg atcgtcccga ccgcggcccc ctgtccgtcg
     1921 tccccatcgg ccatcatcag caggatcgcg tgctcggcgc gcgccttcgc gttgcggagc
     1981 tccgggacgt acggatgcgc ggcgtcgacc aggccgtcgg cgagatacgc gagcgcggtc
     2041 aactcgccga tctccgccag gcgcatggtc tggtccggcc caacagtgac gatgcggctc
     2101 atcgtgcgcc tacccctccg tcgcagcgcg ccgccgcgac gcgggtcggc atgctcctcg
     2161 tagcctagtt tgcctcgcgg cctccggcca gggctcccgc atcaggcaac ggccccgcac
     2221 taggcgacgg ctcccggccc gccggcaagc agcaccacga aaccctcctc gtcgagagtg
     2281 ggcaccccga actccgcagc cttcgcggcc ttcgaaccgg cgttcgcgcc gatgacgacg
     2341 aagttggtct tggcgctcac cgacgacgac gcgcgacccc cgcgcgacgc gatcgcctcc
     2401 gcggcctcct cacgggagaa gccttcgagc gagcccgtga cgacgatggt cagcccggca
     2461 aggacgcgct cgccttccgc tcccccgtca tcctccatcc tgacgcccgc cgccgcccag
     2521 cgcccgacga tctcgacgtg ccagggaacg ccaaaccact ccgcgatgct ccgcgctatg
     2581 acgggcccga cgccgtcggt cgaggagagc gcgcccggat ccgccccgcg aatcgcgtcg
     2641 agcgagccga aggccgttgc gagggcccgc gcggccgtcg ggccgacgtg gcggatgctc
     2701 agggacacga ggacgcgcca cagtgggcgg gacctcgcgg cgtcgaggtt cgcgagcaac
     2761 cgcagtccat tcgtggagac gtccccggcc gtgcgtgcga cgccgtcctt cttggagtcc
     2821 tggtcgaggg tgagcagcgg gacgcatctc aggtcgtcct cggtcagatc gaagaggccg
     2881 gcttcgttgc ggagcacccc cgaatcgacg agcgccgcgg ccgccttctc gccgagcacc
     2941 tccacgtcga gcgctcccct ggacccgatg aaggcgatcc tgtcgacgat ctggccgcgg
     3001 catccctcgg cgttggggca ccgaaggtcg acgtccccgt ccttctgctg ggcgagcgcc
     3061 gtcccgcaac ttgggcacac ggtcggcatc acccacaccc gctccgcgcc ggtgcgcacc
     3121 gcgacaacgg gggccacgat ctcggggatc acgtcgcctg ccttgcgcac cacgacggtg
     3181 tcgccgatca acacaccctt gcgcgcgacc tcctgcgcgt tgtggagcgt cgcaaacgtc
     3241 acggtgctac cggcgacctt cacgggcgtc atcacgccgt acggcgtcac tcggccggtg
     3301 cggcccacat ccacgcggat gtcctcgagc cgagtgttga cctcctccgg ggggaacttg
     3361 taggcgatcg cccatcgcgg agcgcgcgag gtcgaaccga gctcgcgctg cgcgtcgcgg
     3421 tcgttgacct tgatgacggc gccgtcgatc tcgtgcacca gcgcgtggcg gcgcgcctcg
     3481 agccgggaga ggtacgcctc gacctcgccg acccccgtgt ggacctcggc gagctcgctc
     3541 accggcaggc cccattcctt gagcacggag tagatgccga tctgggtcgc ggcccgggga
     3601 tcgggtgccg ccgcgcccca ctcgagcgcg cccagcgcgt acgtcgtgac ggccagcggg
     3661 cgcgtcgccg tgacggccgc gtccttctgg cgaagcgaac ccgccgcggt gttgcgggga
     3721 ttcgcgtacg gggccttgcc ctcctcgact agccgctcgt tcagctcctc gaatgcagcc
     3781 acgggcagat agacctcgcc tcgaatctcc acgacctcgg gcacgccgcc ccccgcgagg
     3841 cggctcggca accccgcgat ggtcctgacg ttggcggtga cgtcctcgcc cgtcgtcccg
     3901 tcgccgcgcg tgaccccgcg caccaggtcg ccgccctcgt aggtgaggga gatggacagg
     3961 ccgtcgatct tcggctcgca gacgaactcg cggggcccac cagcccgctc gacgcgctcc
     4021 tcccacgcgg ccacgtccgc gatcgagaag gcgttgtcga gcgacatcat gcgttcgcgg
     4081 tggcgcacgg acgcgaagcc cgtcgcgacc gcgccgccga ccgtctggct cgggctgtcc
     4141 gggtcggcga gttcgggatg ctcggcctcg atcgcgcgga gctcccgctc aagcgcatcg
     4201 tattcggcgt cggagatggt tggtgcgtcg cgctcgtagt acgcggctcg gtgcgcctcg
     4261 atcgcctgga tgagctcggc gtggcgcgcg cgggggtcgt tcacgggtcc attccaccac
     4321 gccggtgcga ccttggggcg gcgcccacgc acctctcttg ggcgcgagag cgtccgaccc
     4381 aagcgggcct tcggtccgcc gtcccggtgg cctccggcgc tacgatcaaa gcgaatgcac
     4441 cacggaggcg ccatgatcca c
//
LOCUS       DASVKP010000052         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049 k141_13578,
            whole genome shotgun sequence.
ACCESSION   DASVKP010000052 DASVKP010000000
VERSION     DASVKP010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13578"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..547
                     /locus_tag="VF362_01075"
     CDS             149..547
                     /locus_tag="VF362_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier family protein"
                     /protein_id="HEX7589552.1"
                     /translation="MATRRRVSPIEGRAALAQWASGDRASDVTATAVRFALEELAAIA
                     PGGSVEVRIPPHGAAQCIEGAHHTRGTPPNVVETDPDTWLRLVLGDLNWDEATQSGRV
                     SASGARADLAPWLPLFAATRDEQGEASPQP"
     gene            565..930
                     /locus_tag="VF362_01080"
     CDS             565..930
                     /locus_tag="VF362_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589553.1"
                     /translation="MSDATDDLPSQAELDANSLPAFLRRAPRLARIIAIGAGIGFVFG
                     VVIALGLPGHGASYRAVVALLVGLGLAMVGALTAAAIATRPDGRDSRAGAPSWPGMQA
                     IEDAVAAEGKSEPQPGRDG"
     gene            927..2399
                     /gene="purF"
                     /locus_tag="VF362_01085"
     CDS             927..2399
                     /gene="purF"
                     /locus_tag="VF362_01085"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX7589554.1"
                     /translation="MVRRGDGRLSHDLLPGEKGPQDACGVFGIFAPGEEVAKLTFFGL
                     YALQHRGQESAGIATSNGEQILVYKDMGLVSQVFDERALQSLTGHIAIGHTRYSTTGS
                     STWANAQPALGPTAHGTVALGHNGNLTNLPELLELLEERHGQGKLFDFMGGQGSDTAV
                     VTALLANEEYATLAETALELMPRLKGAYSFIFMDETSLYAARDPYGVRPLVLGRLKSG
                     GWVVASESAALDIVGAERVREVEPGEMIIINADGVRSERFAEASPRGCIFEYVYLARP
                     DTSIAGQSVHVARLQMGKTLAREHPVDADLVIPVPESGTPAAVGYAQESGIPFAQGFT
                     KNAYVGRTFIQPSQTLRQLGIRLKLNPLREVIAGKRLVIVDDSIVRGNTQRAIVAMLR
                     DAGAAEVHVRISSPPVLWPCFYGIDFATREELAAVGHETEQVRLSIGADSLGHLSLEG
                     MIAATKQPAHNLCTACFTGQYHIEPPASALHLLAHQDGES"
     gene            2396..>2758
                     /locus_tag="VF362_01090"
     CDS             2396..>2758
                     /locus_tag="VF362_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012804525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEX7589555.1"
                     /translation="MSARAPGPDAITYAGAGVDTAAGDRAVELMKAAVARTHGPEVVG
                     GVGGFAGLFDASALKNYRRPLLASSTDGVGTKIAIARALGIHDTIGRDLVAMVVDDIV
                     VCGATPLVMTDYIACGKVV"
BASE COUNT          414 a         1000 c          948 g          396 t
ORIGIN      
        1 gcgcttcgta gcggacgagg cgccgtgggc agccatgctg ccggaccttg agcccgagtg
       61 gaccgcgtat ctggtcaggg aaggcgtgat cgaccccccc tgacagggag agtgcgtggc
      121 gcccttgtgc ccgtgccact ctaggatcat ggccacgcgt cgccgagtct ccccgatcga
      181 gggccgcgcc gcgctcgccc agtgggcgtc gggcgaccgg gcaagcgacg tgacggcgac
      241 ggcggtcagg ttcgcgctcg aggaactcgc ggccatcgcg ccggggggaa gcgtcgaggt
      301 ccgcatcccg ccgcacggcg ccgcccagtg catcgagggc gcgcaccaca cgcgcggcac
      361 cccgcccaac gtcgtcgaga cggatccgga tacctggctt cgcctcgtcc tcggcgacct
      421 caactgggat gaggcaaccc agagcggccg ggtctcggcc tctggcgcgc gggccgacct
      481 cgcgccgtgg cttccgttgt tcgcggcgac gcgtgacgag cagggcgagg cgtcgccgca
      541 gccatgaccg cctagactga tcccgtgagc gacgccaccg acgaccttcc ctcgcaggcc
      601 gaactcgacg cgaactcgct tcccgccttc ctgcgacggg ccccacgcct cgcccggatc
      661 atcgcgatcg gcgcgggcat cggcttcgtt tttggcgtag tcatcgccct tggccttccc
      721 ggtcatgggg cgtcctaccg cgcggtcgtc gcgctcctcg tggggctcgg gctggcgatg
      781 gtaggcgcgc tcacggcggc cgcgatcgcc acgcggccgg acggacgcga ctcccgcgcg
      841 ggcgcgccgt cctggcccgg catgcaggcg attgaggacg ccgtggccgc ggagggcaag
      901 tccgaaccgc aacccggcag ggacggatag tgcgccgggg cgacggacgg cttagccacg
      961 acctgctgcc cggagagaag ggtccccagg acgcctgcgg cgtcttcggc atcttcgcgc
     1021 cgggcgagga ggtcgcgaag ctgacgttct tcggcctcta cgccctgcag caccgcggcc
     1081 aggagagcgc cggaatcgcg acctcgaacg gcgagcagat cctggtctac aaggacatgg
     1141 ggctggtgag tcaggtcttc gacgagcgcg cgctccaatc gctcacgggc cacatcgcca
     1201 tcggtcacac gcgctactcc accaccggct cgagcacgtg ggcgaacgcc cagcccgcgc
     1261 tcggcccgac cgcgcacggg acggtcgcgc tcggccacaa cggcaacctc accaacctgc
     1321 cggaactcct tgagttgctg gaggagcgcc acgggcaggg gaagctcttc gacttcatgg
     1381 gtgggcaggg gtcggacacc gccgtggtga ccgccctcct cgcgaacgag gagtacgcca
     1441 ccctcgccga gaccgccctg gaactcatgc cgcgcctcaa gggcgcgtac tccttcatct
     1501 tcatggacga gacgtcgctg tacgccgccc gcgatccgta cggcgtgcgc ccgctcgtgc
     1561 tgggacgcct caagagcggc ggatgggtcg tggcgtcgga gtcggcggcg ctcgacatcg
     1621 tcggcgccga gcgggttcgc gaggtggaac cgggcgagat gatcatcatc aacgccgacg
     1681 gggtccggtc cgagcgattc gccgaggcga gcccgcgggg gtgcatcttc gagtacgtct
     1741 acctggctcg tcccgacacc agcatcgcgg ggcagagtgt tcacgtcgcg cgcctccaga
     1801 tgggcaagac gctcgcccgc gagcatccgg tcgacgccga cctcgtgatc cccgtgcccg
     1861 agtcggggac ccccgccgcc gtgggttacg cgcaggagtc cggcattccc ttcgcgcagg
     1921 ggttcaccaa gaacgcctac gttgggcgga ccttcatcca gccgtcccag accctgcggc
     1981 aactggggat tcggctcaag ctcaaccccc tccgcgaggt gatcgcgggc aagaggctcg
     2041 tcatcgtgga cgactcgatc gttcggggaa acacccagcg cgccatcgtc gcgatgctgc
     2101 gggacgcggg tgccgccgag gtgcacgtcc gaatctcgtc gcccccggtc ctgtggccgt
     2161 gcttctacgg catcgacttc gccacccgcg aggaactcgc ggcagtgggg catgagaccg
     2221 agcaggtgcg cctcagcatc ggggccgatt ccctcggcca cctctcgctc gaggggatga
     2281 tcgccgcaac gaagcagccc gcgcacaacc tgtgcactgc ctgctttacg gggcagtacc
     2341 acatcgagcc tccggcgagc gccctccacc tcctcgcgca tcaggacggg gagtcgtgag
     2401 cgcgcgcgcc ccgggccccg acgcgatcac ctacgccggc gcgggcgtcg acaccgcggc
     2461 cggggaccgc gcggtcgaac tcatgaaggc cgcagtcgcc cgcacccatg gccccgaggt
     2521 cgtcggcggc gtcggcggat tcgcgggact cttcgacgcg agcgcactca agaactaccg
     2581 gcgacccctc ctcgcgtcct ccacggacgg ggtgggcacc aaaatcgcca tcgcccgcgc
     2641 gctcggcatc cacgacacca tcgggcggga cctcgtggcc atggtcgtgg acgacatcgt
     2701 cgtgtgcggg gccacccccc tcgtcatgac ggactacatc gcgtgcggga aggtcgtt
//
LOCUS       DASVKP010000053         5545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1331654, whole genome shotgun sequence.
ACCESSION   DASVKP010000053 DASVKP010000000
VERSION     DASVKP010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331654"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VF362_01095"
     CDS             complement(<1..268)
                     /locus_tag="VF362_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7589556.1"
                     /translation="MPILRNELGDVLRGARLTQGKTLRDVSSDARVSLGYLSEVERGQ
                     KEASSELLASICDALDVPMSEVLRDVADRFTVAEALAAAAVPTIP"
     gene            complement(322..819)
                     /locus_tag="VF362_01100"
     CDS             complement(322..819)
                     /locus_tag="VF362_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX7589557.1"
                     /translation="MASAQESALAGARARGLTIATAESLTGGLVAAALTSVPGASDVF
                     LGGLVAYSLDAKGDALGVPRDLLAACGPVSEEVAVAMAEGARRLFGADLAVATTGAAG
                     PKPHGGRPPGTFCVGVASPFGSLARTEVVPGGRHEVRAKATDRAILALCEVIDSAGEP
                     GTRVG"
     gene            complement(823..1395)
                     /gene="pgsA"
                     /locus_tag="VF362_01105"
     CDS             complement(823..1395)
                     /gene="pgsA"
                     /locus_tag="VF362_01105"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX7589558.1"
                     /translation="MTSLVPNALTALRLVGVPALVWLMLADDDAGGSLRWWALLVFLA
                     ASATDFLDGFLARRWSAVSVFGKLADPIADKALILAALAALSVVDGVPWWPFIVLAIR
                     EVAVTVGRFAVMDRGVIAASGGGKVKTTVQIAAVTFYLWPHGPAWLHSVAWWCLLLAV
                     AIAVVTGVQYGRDIVRMRRAAPAPRPVDAR"
     gene            1519..2850
                     /locus_tag="VF362_01110"
     CDS             1519..2850
                     /locus_tag="VF362_01110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX7589559.1"
                     /translation="MVIIAAAIVGVVAIGAVVLAGAGYKFSDLLGRVDAYQLTAAAPR
                     VTPSLDPVVVEPDGPVSFTLVAAGDVLPHGAVVDDAERSGTFTMEPLWSELDPWISGA
                     DLAICHMETPVAPPGTKPSGFPVFSAPPEIVPALKLQGWDGCTTASNHMVDKSWAGIV
                     ASLGAFDAVRMGAVGTSTSPEEASRPQLYKVQAGNRFITVANISWAYGTNGLPVPADK
                     PWSVSLFNANAADASGIIAQAQAARDQGADVVIASVHCCVEYQTEPTAAQRALFQKIA
                     DSGLVDLVIGHHAHVPEPIEKLSGGPGGKGMWAAFGLGNYISNQDYSTVGHTETASGV
                     LLTATFTVAVDDTVDVGVEWTGITVDRTGGHTLHVLSDIPNGVGNLSAAEVAKRYQLV
                     RSAVGPQAPERTTPPTHLADSAFWIERTPFAGRTGTPGPEGPYDPIQWPTG"
     gene            complement(2847..5102)
                     /locus_tag="VF362_01115"
     CDS             complement(2847..5102)
                     /locus_tag="VF362_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7589560.1"
                     /translation="MDGLVGLVQQPAFIVAASILLLAVTVMSFVAIHRTGKAREARDV
                     ATAQAAGLRRQLEAVLASARDGVVVTSAAGDIIMMSEAAGECLGVSATEAIGRPVAWL
                     GVRTIDAGMHPLNLQEVLSARYSRGDGPRVVGVPGRRGGDDVRWLQVSTVEVPDADPS
                     GPVSVALIVDSTGTSDATEATNRSDQQFRKAIESAPLPMALVDLEWRLMEVNRAFAEM
                     LGSTVSALRGTPFAALSHPADVSKEREHLQRLYDGSEPRFAIEKRFVKSDGLVAYASL
                     SVGLVRHPGGAPDHYIVQLRDTTQDRVHSDQLAHAVEHDPLTRLANRTLMNDVLEKAV
                     GQPDAIGRVAVLAVDLDGFKGLNDRFGHAVGDDALFHTARVLEAAAGRHGKVARIGGD
                     EFAVIIDEADAQVSAVQVAEAIHEGLRKPLSLKRHQLSLHASVGIALADSGTLADGMA
                     SLLNAADAAMYRAKAAGKSRTEMYVPSMRIDAEAHGALISELRQAIDHGALVLHYQPI
                     LELSDRTVVGYEALVRWQHPTRGLLLPGAFLPLMNDRQLSADLGAALVEQVAEFLAEN
                     RDRHTWVSLNVSADQLGDSEFADRVLTSIARHNLSPHRLVIELTEASLVSPNTRVRHE
                     LTELRNAGIPILLDDFGTGVSPLSYLRDLPVSGVKLDMSFTAGIPEDPAGARVSRALG
                     ALARELGLATIAEGIETESQAEYLRSCGWRYGQGWLFGVAQPASATPDASVTFSQSFI
                     DPRPNDDEMAG"
     gene            complement(5087..>5545)
                     /locus_tag="VF362_01120"
     CDS             complement(5087..>5545)
                     /locus_tag="VF362_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009885082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX7589561.1"
                     /translation="FLPMGESKAVRIQGAWVSESEIHAAVEHVKTQAQPRYREDVVLA
                     QAASRPAQEIGDDLEELLAAAELVITTQLGSTSMLQRKLRVGFAKAGRLMDILETREI
                     VGPSQGSKAREVLVTPDRLPATLAILRGEVPTPLGGTAAIVDGDGGWTDS"
BASE COUNT          896 a         1951 c         1884 g          814 t
ORIGIN      
        1 ccgggatggt cggcacggcg gcggcggcga gggcctccgc cacggtgaag cggtccgcga
       61 cgtcgcgcag gacctcagac atcggaacgt cgagcgcatc gcagatggaa gccaggagtt
      121 cggacgacgc ctccttctga ccgcgctcaa cctcggagag gtacccgagc gacacccggg
      181 cgtccgagga tacatcgcgc agggtcttgc cctgcgtcag gcgcgcgccg cggaggacgt
      241 cgccgagttc attccgaaga atgggcattt gtcctccctt tcgcaagatc cgagtgctct
      301 ccacgtctga cacaacgcag actaccccac ccttgttccc ggctcaccag ccgagtcgat
      361 aacctcacac aacgccagaa tcgcccgatc cgtcgccttt gccctgacct cgtgcctgcc
      421 gcccgggacg acttccgtcc gcgcgaggga gccgaacggg ctcgcgacgc ccacacagaa
      481 cgttccgggg ggcctgccgc cgtgcggctt cggccctgcc gcgcccgtcg tggcgacggc
      541 aagatcggcg ccgaacagac ggcgcgcgcc ctcggccatc gcgaccgcca cctcctcgct
      601 gaccgggccg catgccgcga gcagatctcg gggcacgccg agcgcatctc ccttcgcgtc
      661 gagggagtag gcgacgaggc cgccaaggaa cacatcggac gcgccgggca ccgaggtcag
      721 ggcggccgcg acgagccctc ccgtgagcga ctccgcggtc gcgatcgtca ggcccctggc
      781 acgcgcgccc gcaagcgccg actcctgcgc gctcgccacg ggctacctcg cgtcgacggg
      841 tcgaggcgcc ggcgcggcac gcctcatgcg gacgatgtcc ctgccgtact gcacgccggt
      901 caccacggcg atcgcgacgg ccagcaggag gcaccaccac gcgacggagt ggagccacgc
      961 gggcccgtgg ggccacaggt agaacgtcac ggcggcgatc tgcaccgtcg tcttgacctt
     1021 gcctccgccc gacgccgcga tcactccccg atccatcacg gcgaagcgcc cgaccgtaac
     1081 cgcgacttcg cgaatggcca gcacgatgaa cggccaccac ggcacgccgt cgaccacgct
     1141 cagcgcggcg agcgcggcga ggatgagggc cttgtcggcg atgggatccg cgagcttccc
     1201 gaacacgctg accgcgctcc agcgacgcgc gaggaagccg tcgaggaagt ccgtcgcgga
     1261 cgcagcgagg aacaccagga gggcccacca ccgcagggat cccccggcat cgtcatcggc
     1321 gagcatcagc cacacgagcg cagggacgcc cacaagccga agcgcggtca gggcgttcgg
     1381 gacgagggag gtcattccct caccctagtg cgggcgcgcg ctcgccgagc caaggggagg
     1441 taaggtgccc tcatggcgcg ccatacccat cggactccac cgacgcacga cgaaccccgc
     1501 cgacgagtcc gccgcacaat ggtgatcatc gccgccgcga ttgtcggggt cgtcgcgatc
     1561 ggcgcggtgg ttctggccgg ggcgggatac aagttctctg acctgctggg aagggtagac
     1621 gcgtatcaac tgaccgccgc cgccccccgc gtcaccccct cactcgaccc cgtggtcgtg
     1681 gagcccgacg gccccgtgtc cttcacgctc gtcgccgcgg gggacgtcct cccgcacggg
     1741 gcggtcgtcg acgacgcgga gcgaagcgga acgttcacca tggaaccgct ctggagcgaa
     1801 ctcgatccgt ggatctcggg cgccgacctc gcgatttgcc acatggagac ccccgtcgcg
     1861 ccgccgggca ccaagcccag cggcttccca gtgttctccg cccccccgga gatcgtgccg
     1921 gccctcaagt tgcaggggtg ggacggctgc acgaccgcgt cgaatcacat ggtcgacaag
     1981 agttgggcgg gcatcgtggc gagcctcggc gcgttcgacg ccgttcgcat gggcgccgtc
     2041 gggacctcga cgagccccga ggaggcgtcc cggccccagt tgtataaggt ccaggccggg
     2101 aaccgattca tcacggtcgc gaacatctcg tgggcgtacg ggacgaatgg cctgcccgtc
     2161 cctgccgaca agccctggtc cgtgagcctc ttcaacgcca acgccgcaga tgcgagcggg
     2221 atcatcgcgc aggcacaggc cgcccgagac caaggcgccg acgtcgtcat tgcgtccgtc
     2281 cactgctgcg tcgaatacca gaccgagccc acggccgccc agcgggcgct gttccagaag
     2341 atcgcggact ccgggctcgt cgacctcgtg atcggccacc acgcgcacgt gccggagccg
     2401 atcgagaagc tctccggagg tccaggaggc aagggaatgt gggcggcgtt cgggctcggg
     2461 aactacatct ccaatcagga ctactcgacg gtgggccaca ccgagaccgc aagcggcgtg
     2521 ttgctcacgg ctaccttcac ggtcgccgtc gacgacaccg tcgacgtggg agtggagtgg
     2581 accgggatca cggtcgatcg cacgggtggg cacacgcttc acgtgctgag cgacattccg
     2641 aacggcgtcg gcaatctctc ggccgcggag gtggcgaagc ggtaccaact ggtgaggagt
     2701 gctgtcgggc cgcaggcgcc cgagcggacg acccctccga cccacctggc cgactccgcc
     2761 ttctggatcg aacggacccc cttcgcggga aggacgggca cccccggtcc cgagggcccg
     2821 tacgatccca tccagtggcc tacgggctag ccagccatct cgtcgtcgtt agggcgcggg
     2881 tcgatgaaac tctgcgagaa ggtgacggac gcgtcgggtg tcgccgaagc tggctgggcc
     2941 accccaaaca gccagccctg accgtagcgc catccgcacg aacgcaggta ctcggcctgc
     3001 gactcggtct cgattccctc ggcgattgtc gccaaaccga gttcccgcgc caaggctcca
     3061 agggccctcg agacgcgggc gcccgccgga tcctccggta tccccgcggt gaaggacatg
     3121 tcgagtttca cgcccgacac ggggaggtcc ctcaggtagg agagcggaga gaccccggtc
     3181 ccgaagtcgt cgagcaggat ggggatcccc gcgttgcgca attcggtcag ctcgtgccgg
     3241 acacgtgtgt tgggtgagac gagcgacgcc tcggtcagtt cgatgacaag acggtgcggc
     3301 gagagattgt ggcgcgcgat gctcgtgagc acccggtcgg caaactcgct gtccccgagc
     3361 tggtcggcag aaacgttcag ggagacccac gtgtgcctgt ctcggttctc cgccaggaac
     3421 tccgcaacct gttcgaccag cgcggcgccg aggtccgcgc tcagctggcg gtcgttcatg
     3481 agggggagga aggctccggg caggagaagg ccgcgcgtcg ggtgctgcca ccggaccagg
     3541 gcctcgtagc ccacgacggt ccgatcggac agttcgagga tcggctggta gtgaaggacg
     3601 agggcgccgt ggtcgatcgc ctgccgaagc tccgagatca gcgccccgtg ggcctcggcg
     3661 tcgatccgca tggacggcac gtacatctcg gtacggctct tgcccgcagc cttcgcgcgg
     3721 tacatcgcgg catcggccgc gttgagcagg ctcgccatgc catccgcgag tgtgccggaa
     3781 tccgcgagcg caatgccgac gctcgcgtgc aggctgagct ggtggcgctt gagggacagc
     3841 ggcttgcgta gcccctcgtg gatggcctcc gccacctgga ctgcggagac ttgcgcgtcg
     3901 gcctcgtcga tgatgaccgc gaactcgtcg ccgccgatgc gcgcgacctt cccgtggcgc
     3961 cccgcggcgg cctcgagcac tctcgccgtg tggaagagcg cgtcgtcgcc caccgcgtgg
     4021 ccgaaccggt cgttgaggcc cttgaatccg tcgaggtcga ccgcgagaac cgcgaccctt
     4081 ccgatggcgt cgggctggcc gaccgccttc tccaggacgt cgttcatgag cgtgcggttg
     4141 gcgagtcgcg tgaggggatc gtgctcgacc gcatgggcga gctggtcgga gtggacgcgg
     4201 tcctgcgtcg tgtcgcgcag ttgcacgatg tagtggtccg gggcgccgcc gggatggcgc
     4261 accaacccga cgctgaggga cgcgtaggcg acgaggccat ccgacttgac gaatctcttc
     4321 tcgatggcga agcgcggctc cgagccgtcg tagagccgct ggagatgctc gcgttccttc
     4381 gacacatcgg cggggtgaga cagcgcggcg aagggggtgc cgcggagggc cgaaaccgtt
     4441 gagccgagca tctccgcgaa ggcgcggttc acctccatga ggcgccactc aaggtcgacg
     4501 agcgccatcg gaagcggcgc gctctcgatc gcctttcgga actgctgatc ggaccgattt
     4561 gtggcctccg tcgcgtcgct cgtgcccgtc gagtcgacga taagggcgac gctcaccggc
     4621 ccgctcgggt cggcatccgg aacctcgacc gtcgatacct ggagccaccg gacgtcgtcg
     4681 ccgccacgcc ttcccgggac cccgacgacc ctcgggccgt caccgcggct gtatctcgcg
     4741 gagagcacct cttgaaggtt gagcgggtgc attcccgcgt cgatcgtcct caccccgagc
     4801 caggcgacgg ggcgcccgat tgcctcggta gcgctcaccc cgaggcactc gccggcggcc
     4861 tccgacatca tgatgatgtc tccggccgcg gaggtcacga cgacgccgtc ccgggcggag
     4921 gcgagcacgg cctccagttg tcgccgcagg cccgcggcct gcgccgttgc cacgtccctc
     4981 gcctcgcgcg ccttgcccgt gcggtggatc gccacgaaac tcatgaccgt aacggcgagc
     5041 agaaggatgc tcgcggcgac gatgaaggcc ggctgttgta cgaggcctac gagtccgtcc
     5101 atccaccgtc tccatcgacg atcgccgcgg tccccccgag gggcgtgggg acctcccctc
     5161 tgaggatagc gagcgtcgcg ggcagacggt caggtgtgac gagaacctca cgagccttgg
     5221 agccctgcga ggggccgaca atctccctgg tctccaagat gtccatgagt cggcccgcct
     5281 tggcgaagcc cactcgtagt ttgcgctgga gcatggaggt ggaccccagc tgggtggtga
     5341 tgacgagctc cgcggcggcg agcaactcct cgaggtcgtc gccgatctcc tgcgccggac
     5401 gggacgccgc ctgcgccaag accacgtcct cgcggtatcg cggctgcgcc tgggtcttga
     5461 cgtgctcgac ggcggcgtgg atctcgctct cgctcaccca cgcaccctgg atgcgcacgg
     5521 ccttcgactc ccccatcgga aggaa
//
LOCUS       DASVKP010000054         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_837807, whole genome shotgun sequence.
ACCESSION   DASVKP010000054 DASVKP010000000
VERSION     DASVKP010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837807"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VF362_01125"
     CDS             <1..669
                     /locus_tag="VF362_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX7589562.1"
                     /translation="EALEPAWTVVHDESEIAGRAGAALLVRGEARDPVVRLPGLPGVP
                     SHTGRWAEAKVATPHGDVRAVSVYVHSGQADTPKMDDKYAFLDAMSARMRELLAAHER
                     VVVMGDINIAHAPRDLKNWKANLKTAGFLAEERAYLDAWLAEGWVDVHRTLSGDVDGP
                     YTWWSQRGKAFDNDVGWRLDYQFASPRLAALASEARVDRAASWDSRWSDHAPLVVRYS
                     ALNS"
     gene            complement(648..1883)
                     /locus_tag="VF362_01130"
     CDS             complement(648..1883)
                     /locus_tag="VF362_01130"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HEX7589563.1"
                     /translation="MVAMSDSRGVGAPAWNEPWTRAEGEERARALFATVYDEEPTGAW
                     SAPGRINFVGDHTDYNGGLCLPAIIRHRAYVAGRARPDAIVRISDGHDPARTIEIEID
                     AISEEVHHTWLGAAAGVVGAMVERGFTGPGLDLAVASCVPQRVGLASTAAFACATALA
                     VNDLWRLALDTPALRVDLAEAAYDAESLVVGESIGRLNEYVSLFGSRHNALLLDCSTS
                     PPAMHDTVLSFREHGLEVVVVDTGTTYGVADAGARRAECVAATSALGARSLREIADAP
                     NGLSLVEALSDPILRKRARHVITEIHRVRGVVDALAGVGPAHERFVEAGRLISASHAS
                     LAEDFEVSGPELDLAVEASQAAGALGAKLVGVGFGGAVIALVRRTARATLVDSVDRAF
                     ADAGFPPPRYLLPYEFSAE"
BASE COUNT          323 a          700 c          707 g          270 t
ORIGIN      
        1 gaggccctcg agcccgcgtg gaccgtcgtc cacgacgagt ccgagatcgc ggggcgcgcc
       61 ggggctgcgc tcctcgtacg cggcgaggcg agggatcccg tcgtgcgcct gcccggcctt
      121 cccggggtcc cctcacacac gggccggtgg gccgaggcga aggtggcgac gccgcacggc
      181 gacgtgcgcg cggtctccgt ctacgtccac tcggggcagg ccgacacgcc caagatggac
      241 gacaaatacg ccttcctcga cgcgatgagc gcgcgaatgc gagagctcct cgccgcacac
      301 gagcgggtgg tcgtgatggg cgacatcaac atcgcccacg ccccgcgcga tctcaagaac
      361 tggaaggcca acctcaagac ggcgggcttc ctcgcggagg agcgggcgta cctcgacgcg
      421 tggctcgcgg agggatgggt cgacgtccat cggaccctgt cgggcgacgt ggacgggccg
      481 tacacgtggt ggtcgcaacg cggcaaggcc ttcgacaacg acgtcggatg gcgcctggac
      541 taccagttcg cctcgccgcg actcgcggcc ctcgcctcgg aggcgcgggt cgacagggcg
      601 gcttcttggg acagccggtg gagcgaccac gcgccgttgg tcgtccgcta ctcggcgctg
      661 aactcgtagg ggagcaggta gcgcgggggc gggaagccag catccgcgaa cgcgcgatcc
      721 acggagtcca cgagggtcgc cctggccgtg cgccgaacga gcgcgatcac ggctccgccg
      781 aaccccaccc cgacgagctt ggcgcccagg gcgcctgccg cttgcgacgc ctcaaccgcg
      841 aggtcgagtt cgggaccgct cacctcgaag tcctcggcaa gtgaggcgtg cgacgccgag
      901 atcaggcggc cagcctcgac gaatcgctcg tgagcggggc cgactcccgc gagggcgtcg
      961 actacccccc tgacgcggtg tatctcggtg atgacgtgtc gggcgcgctt gcggaggatg
     1021 gggtcgctga gcgcctccac gagcgaaagg ccgttcgggg cgtcggcgat ctcgcgcagg
     1081 ctccgcgccc cgagcgccga cgtcgcggca acgcactcgg cccgccgtgc ccccgcgtcc
     1141 gcgacgccgt acgtcgtgcc ggtgtcgaca acgacgacct caagtccatg ctcgcggaaa
     1201 gagagcacgg tgtcgtgcat cgccgggggc gacgtggagc agtcgagcag gagcgcgttg
     1261 tgcctcgatc cgaagaggga gacatactcg ttgaggcgcc cgatcgactc tccgacgacg
     1321 aggctttcgg cgtcgtacgc cgcctccgcg aggtcgacgc ggagcgccgg cgtgtcaagc
     1381 gcaagacgcc agagatcatt cacggcgagc gccgtcgcgc acgcgaacgc cgcggtggag
     1441 gcgagcccga cgcgttgcgg aacgcacgac gccaccgcca ggtcgaggcc cggcccggtg
     1501 aatccgcgct cgaccatcgc gccgacgaca cccgcggcgg cgccgagcca cgtgtggtgc
     1561 acctcctcgg aaatcgcgtc gatctcgatc tcgatcgtcc gcgccggatc gtgcccgtcc
     1621 gagattctca cgatcgcgtc gggtcgcgcg cgcccagcga cgtaggcgcg gtgccggata
     1681 atggctggca ggcagagacc gccgttgtag tccgtgtgat ccccgacgaa gttgatgcga
     1741 cccggcgccg accaggcccc cgttggttcc tcgtcgtaga cggtcgcgaa cagcgcgcgc
     1801 gcccgctcct caccctcggc gcgtgtccag ggctcgttcc acgccggggc gccaacgccc
     1861 cttgagtcgc tcatcgccac catgttagtc aaccggtacc ggtcctatcc gaccgaacgg
     1921 gctcgtgcca tgagggcggt aatcgcccct aaggggacaa cgcgaacaaa ggagacagat
     1981 gatggcccaa gagaagcagg
//
LOCUS       DASVKP010000055         4592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_180938, whole genome shotgun sequence.
ACCESSION   DASVKP010000055 DASVKP010000000
VERSION     DASVKP010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180938"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VF362_01135"
     CDS             <1..1127
                     /locus_tag="VF362_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589564.1"
                     /translation="SQNRRDAEEIRAGAESETALLRETTARETSELAERTNRDVESQR
                     AGIAGELNEKRASAEREISALMATAEREATELRVGSQRDAQALAETSGIEAERLVSDA
                     KAQAASIMGEARTERDKTRQETLRLREDLQVEAAQKREDAEQQLKRRYEEANVEYEKL
                     VADAQERAAEGERRAREALERSGVVRSEAEAHADTLLANARKNADEIITDAREHAEKI
                     MSEAVNDAERERSAALTAVDGLNRQRESITSYLDELRTLLVQDPNATRSPERTAALAA
                     VLDGGPVAAPEPDLGLALPSVFTPVDQLVNPFNDRVPVVVVPEAGASVGAEPASEEPA
                     SDEPASEDSGPDEPVFDQPLADEPDADEDPESPSPFRLDN"
     gene            1142..1585
                     /locus_tag="VF362_01140"
     CDS             1142..1585
                     /locus_tag="VF362_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589565.1"
                     /translation="MTTSPADRDAGDRDATAERAFARADGLGRARAAESRAAAVLIKD
                     FTRRAASAGLATTQLRARSYNGNARYRTNVEGWYLRRDHSIGVGADGRFYVLSTPTSL
                     AARVTGVVLHPSDAPLELGRGARDGESIPLADALEKRLAAGDAFP"
     gene            complement(1582..2391)
                     /locus_tag="VF362_01145"
     CDS             complement(1582..2391)
                     /locus_tag="VF362_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7589566.1"
                     /translation="MSIPQRVLHVGLRGAFPAAGTPGRPPVVLLPAFPLTSRMWVPLE
                     RELAARGITAHGFDYPGFGTTPLWTDTEPSIDAIADAAVATLRGGIGAAAAHWVGCSM
                     GGYVALAIAQRHPDAVAGLGLLDTKSSADDDAKRAARLDTARSIEGERTFPHPRAAAE
                     GLVGMEGEGREAVVKEAEAIIAETVPAAAAWGQRAMAGRQDRTGVLEGFRGPSVVAWG
                     SLDSVTSRDDADRMARALGSHTVVIDGAGHLSPLEAPRAAAAAVASLFERP"
     gene            complement(2388..3143)
                     /locus_tag="VF362_01150"
     CDS             complement(2388..3143)
                     /locus_tag="VF362_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7589567.1"
                     /translation="MAGLRSTTVLPAVREDIELRTDDGLTLVGELALPVSGRIEATLV
                     TLHPLPTHGGFMDSHLLRKASWRLPYLADIAVLRFNTRGTHSPRGTSQGAYEGGAGEG
                     LDARAAVAFAAERGLPRRWLLGWSFGTEVALRHGSSLDVEGAILLSPPMQSATATDLA
                     DWRASGKPVLALIPEHDDYLPPDRARAAFAALPSAEVVPVAGAGHLWVGERYVRIVLD
                     QIVARIAPSRSPLPTEVPASAVWHGHATEEDSA"
     gene            complement(3151..3456)
                     /locus_tag="VF362_01155"
     CDS             complement(3151..3456)
                     /locus_tag="VF362_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589568.1"
                     /translation="MARRPRKRPYGDPHPELDVEKLTSGFPARIEPGAGGEPFYVAIS
                     RQRDWTYICPACGQEIPAMTNHVVAWAAGGLFGPEVAADERRHWHTNCWEKFGRARG"
     gene            complement(3498..>4592)
                     /locus_tag="VF362_01160"
     CDS             complement(3498..>4592)
                     /locus_tag="VF362_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEX7589569.1"
                     /translation="TLYHWDLPQPLEDAGGWPNRDTAYAFEAYARRAAEEFKDRVNFW
                     TTLNEPWCTAFLGYSQGSHAPSRTEPVASLAAAHHLNLAHGLASRAIKEVAPDASVSI
                     VLNTHVPRPWDPSNPADVDASRRIDALANRIFMDPLVTGTYPADLIEDTRALTDWSFV
                     RDGDMEIVRGTVDLVGVNYYSSHVVRFNADLEFGDGADGHSPAPGTAWPGSEQVEFMR
                     PFGPLTTMGWNQDASAFHAYLLRMHRDMGLPIVVTENGASFDDVVSPDGRVHDTDRVR
                     YLHDHFAAVLLALRDGVDIRGYMVWSLMDNFEWSHGYSKRFGLLRADFDTLARTWKDS
                     AYWYQETIRTRRLTAVERAAELGTTPPASY"
BASE COUNT          703 a         1613 c         1697 g          579 t
ORIGIN      
        1 tctcgcagaa ccggcgcgac gccgaggaga tccgcgcggg ggcggagtcc gagacggcgc
       61 tcctgcgaga aacgaccgcg cgcgagacgt cggaactggc cgagcggacg aaccgcgacg
      121 tcgagagcca gcgggccggg atcgcgggcg agctcaacga gaagcgggcc tccgccgagc
      181 gggaaatcag cgccctcatg gcgacggcgg agcgggaagc gacagagttg cgagtcggca
      241 gccagcgcga cgcccaggca ctcgccgaga cgtccggcat tgaggccgag cgcctcgtga
      301 gcgacgccaa ggcgcaggcg gcgagcatca tgggcgaggc acgcaccgag cgcgacaaga
      361 cccgccagga gaccctgcgt ctccgcgagg accttcaggt cgaggccgca cagaagcgcg
      421 aggacgcaga gcagcaactc aagcgccggt acgaagaggc gaacgtcgag tacgaaaagc
      481 tcgtcgccga tgcccaggaa cgggcggcgg aaggcgagcg tcgcgcgcgg gaggcccttg
      541 agcgctcagg ggtggtgcgc tccgaggcgg aggcgcacgc ggacacgctc ctcgccaacg
      601 cgcgcaagaa cgccgacgag atcatcaccg acgcccgcga acacgccgag aagatcatga
      661 gcgaggcggt caacgacgcg gagcgcgagc ggagcgcagc gctgacggcg gtggatggcc
      721 tcaaccgtca gcgagaatcc atcacctcct acctggacga gttgcggacc ctcctcgtcc
      781 aggatcccaa cgccacgcga tcgcccgaac gcactgcggc cctcgcggct gtgctcgacg
      841 gtggccccgt tgccgcgccg gagcccgacc tggggctcgc ccttccgagc gtcttcaccc
      901 ccgtcgatca gctcgtcaac cccttcaacg acagggttcc ggtggtagtg gttccagagg
      961 ccggcgcgtc ggttggggcc gagcccgcct ccgaggagcc cgcctcggac gagcctgcga
     1021 gcgaggactc ggggcccgac gagcccgtct tcgaccagcc ccttgccgac gagcccgacg
     1081 ccgacgagga ccccgagtcc ccttcgccct tccggctcga caactagccg tctgcagcgg
     1141 cgtgaccacg tctcccgccg accgcgatgc cggggatcgc gacgccaccg ccgagcgtgc
     1201 cttcgcgcgt gccgatggcc tcgggcgcgc cagagccgcc gagagccgtg ccgccgcagt
     1261 cctgatcaag gacttcactc ggcgtgccgc gtccgcaggc ctcgccacta cgcaattgcg
     1321 cgcgaggagc tacaacggca acgcgcgcta ccgcacgaac gtcgaggggt ggtacctgcg
     1381 gcgagaccac tcgatcggcg tcggcgccga cggacgcttc tacgtcctct ccacgcccac
     1441 gagcctcgcg gcgcgagtga cgggcgtcgt ccttcaccca agcgatgcgc cgctcgagct
     1501 gggcaggggc gctcgcgacg gagagtcgat ccctctcgcc gacgcgctgg agaagcgcct
     1561 cgcggccggc gacgcgttcc cctaggggcg ctcgaacagg ctcgccacgg cggccgcggc
     1621 ggcccgaggc gcctcgaggg ggctcaggtg gccagcccca tcgatgacga ccgtgtggga
     1681 gcccagcgcg cgcgccatgc gatcggcgtc gtcgcggctg gtgaccgagt cgaggctgcc
     1741 ccaagcgacg acggacggcc cgcggaagcc ctcgagcacc ccggtgcgat cctggcgccc
     1801 tgccatcgcc cgctggcccc aggcggccgc cgcgggcacc gtctccgcga tgatcgcctc
     1861 ggcctccttg acgacggcct cgcgaccctc tccctccatg ccgacgaggc cctccgccgc
     1921 ggcgcgcgga tgggggaagg tcctctcgcc ctcgatcgag cgcgccgtgt cgaggcgcgc
     1981 ggcgcgcttg gcgtcgtcgt cggcggagga cttcgtgtcg agcagcccga gccccgcgac
     2041 cgcgtcagga tggcgctggg cgatcgcgag ggccacgtag ccccccatcg agcagcccac
     2101 ccagtgtgca gcggcggcgc cgatgccccc gcgcagcgtc gcgacggccg cgtcggcgat
     2161 cgcgtcgatg ctgggctccg tgtcggtcca caggggcgtc gtgccgaaac ccgggtagtc
     2221 gaacccgtgc gccgtgatac cgcgcgccgc cagctcgcgc tcgagcggga cccacatgcg
     2281 cgaggtgagc gggaaggcgg gcagcaggac gacgggaggg cggccggggg tccccgccgc
     2341 agggaaggcg ccccggaggc cgacatgcag gacgcgctgc ggaatgctca tgcggaatcc
     2401 tcctcggtgg cgtgcccgtg ccacactgcg gacgcgggca cctcggtggg gagaggggag
     2461 cgcgagggcg cgatcctcgc gacgatctgg tcgagcacga tgcgcacgta gcgctccccg
     2521 acccacaggt gccccgcgcc cgcgacggga acgacctcgg cggacggaag ggcggcgaac
     2581 gcggcgcggg cccggtcggg aggcaggtag tcgtcgtgct cagggatcag ggccaggacg
     2641 ggcttgccgc tcgcgcgcca gtccgcgagg tccgtggcgg tcgccgactg cataggcggc
     2701 gacaggagga tcgccccctc gacgtcgagc gaactcccat gccggagcgc cacctcggtc
     2761 ccaaacgacc acccgagcag ccagcggcgc gggaggcccc gctcggccgc gaaggcgacg
     2821 gcagcgcgcg catcgagccc ctccccggcc ccgccctcgt aggcgccctg cgacgtgccg
     2881 cggggcgagt gcgtcccgcg cgtgttgaaa cgcaggacgg cgatgtcggc gaggtacggg
     2941 aggcgccacg aggccttgcg caggaggtgg gaatccatga acccgccgtg cgtgggcagc
     3001 gggtgaagcg tgacgagggt cgcctcgatc cttcccgaga cggggagcgc gagttcgccc
     3061 acgagggtca ggccgtcgtc cgtgcgcaac tcgatgtcct ccctgacggc ggggagcacg
     3121 gtggtggagc gcagccccgc catcgccgcc tcacccacgg gccctcccga acttctccca
     3181 gcagttcgtg tgccagtgac ggcgctcgtc tgccgcgacc tcggggccga acagtccccc
     3241 ggctgcccag gcgacaacgt ggttggtcat cgccggaatc tcctggccgc acgcggggca
     3301 gatgtaggtc cagtcgcgct ggcgcgagat cgccacgtag aagggctccc cgcccgcgcc
     3361 cggctcgatg cgcgccggga agccgctcgt gagcttctcg acgtccagct cgggatgagg
     3421 atccccgtag gggcgcttgc ggggccggcg tgccatgcgg ccagtctacg tttcgcgatc
     3481 cgacgccatg ccgcgcccta gtagctcgct gggggcgtcg tccccaactc cgccgccctc
     3541 tcgacggcgg tcagccggcg cgtccggatc gtctcctggt accagtacgc cgagtccttc
     3601 caggtacgag ccagggtgtc gaagtcggcg cggagcaggc cgaagcgctt ggagtatccg
     3661 tgcgaccact cgaagttgtc catgagcgac cacaccatgt acccgcggat gtcgacgccg
     3721 tcgcgcaggg cgagaagcac ggccgcgaag tggtcgtgaa gatagcggac gcggtcggtg
     3781 tcgtgcaccc ggccgtccgg ggacacgacg tcgtcgaagg acgcgccgtt ctcggttacc
     3841 acgatcggaa gtcccatgtc gcggtgcatc cgcagcaggt aggcgtggaa ggcggaggcg
     3901 tcctggttcc aacccatcgt cgtgaggggg ccgaagggcc gcatgaactc gacctgctcg
     3961 gagcccggcc atgccgtgcc gggggcgggc gagtgcccgt ccgcgccatc cccgaactcg
     4021 aggtccgcgt tgaagcggac gacgtggctc gagtagtagt tcacgccgac gaggtcgacg
     4081 gttccgcgga cgatctccat gtcgccgtcg cggacgaagg accagtccgt gagcgcgcgc
     4141 gtgtcctcga tgaggtcggc ggggtaggtc ccggtgacga gcgggtccat gaagatgcgg
     4201 ttggcgagcg cgtcgatgcg gcgagaggcg tcgacgtccg cggggttgga cgggtcccag
     4261 gggcgcggga cgtgggtgtt gagcacgatg gagacggaag cgtccggggc aacctccttg
     4321 atggcgcggc tcgcgaggcc gtgcgcgagg ttgaggtggt gggcggccgc gagcgaggcc
     4381 acgggctccg tcctgctcgg cgcgtggctc ccctgcgagt acccgaggaa cgccgtgcac
     4441 cacggctcgt tgagcgtggt ccagaagttc acgcggtcct tgaactcctc ggccgcgcgg
     4501 cgcgcgtagg cctcgaacgc gtaggcggtg tcgcggttcg gccagccccc cgcgtcctcg
     4561 aggggttgcg ggaggtccca gtgatagagc gt
//
LOCUS       DASVKP010000056         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1578201, whole genome shotgun sequence.
ACCESSION   DASVKP010000056 DASVKP010000000
VERSION     DASVKP010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578201"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VF362_01165"
     CDS             <1..593
                     /locus_tag="VF362_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX7589570.1"
                     /translation="SAGSLAEYERALDARWSEIGPLDTWVGRLARARASGMVERLAGY
                     VALVKVDEVRTEVPFRIEIGRAVLQGRADRLHVTGDRAVVVDLKTGARAVAPQEAATN
                     AQLAMYQLAASSGGFEGVASAKGAELVYVGVDAVAAAVRTQEPLDVGVAAARLGDVVE
                     AMAASGFEARLGPLCDYCSVRRCCPAQPEGAEVSSS"
     gene            590..>1518
                     /locus_tag="VF362_01170"
     CDS             590..>1518
                     /locus_tag="VF362_01170"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX7589571.1"
                     /translation="MSAGVVKGRGRTVSDLAALVDPTRIPTEEQARVIGWGARPALVV
                     AGAGSGKTETLSMRMVFLLDHAREIWGEDISPDEILCLTFTRKAAAEIADRARRRLDS
                     AFGADAARPVPTVATYNAYSAGLVGEHGLRVGVDPDSVVLTDASLWQMASAIVEGWAD
                     DLDADSAVSSIIGAVPRLAAQLSDHGASPGDLARVCEAIAQTVGSIPSGALRGGGAPT
                     KAQRTEAAKFRARAAMAPLLEEFARRKRDGSFLDFADQIALAGRLARIPAVRAVERSR
                     YRVVLLDEFQDTSHGQMDLFAVMFGQSHPVMAVG"
BASE COUNT          200 a          531 c          568 g          219 t
ORIGIN      
        1 tgtccgcggg ttcgctcgcg gaatacgaac gcgccctcga tgcgcggtgg tcggagatcg
       61 gccccctcga cacgtgggtg ggtcgcctag cgcgggcgcg ggcgtccggc atggtcgagc
      121 ggctcgcggg ctacgtcgcg ctggtcaagg tcgacgaggt ccgcaccgag gttccgttca
      181 ggatcgagat cgggagggcg gtgctccagg ggcgcgccga ccggctccac gtcacgggcg
      241 accgcgccgt cgtcgtcgac ctcaagaccg gggcgcgggc ggtcgcgccc caggaggccg
      301 cgaccaacgc gcagctcgcg atgtaccagc tcgccgcgtc ctcggggggg ttcgagggcg
      361 tggcgagcgc caagggagcc gaactcgttt acgtcggcgt cgacgcggtg gcggccgcag
      421 tgcgcacgca ggaaccgctc gacgtcggcg tggccgccgc gaggctcggc gacgtcgtcg
      481 aggcgatggc ggcgtcgggc ttcgaggcgc ggttgggacc gctttgcgac tactgctcgg
      541 tacgccggtg ctgccccgcc cagccggagg gtgcggaggt gagcagctca tgagcgcagg
      601 cgtcgtgaag ggcagggggc ggacggtctc ggatctcgcg gccctcgtcg accccacgcg
      661 tatcccgacg gaggaacagg ccagggtgat cgggtggggc gcgaggccag ccctcgtcgt
      721 cgctggcgcg ggctccggca agacggagac gctgtcgatg cggatggtct tcctgctcga
      781 ccacgcgcgc gagatctggg gcgaggacat ctcccccgac gagatcctgt gcctcacctt
      841 cacgcgcaag gccgcggccg agattgccga ccgggcgaga aggcgcctcg actccgcgtt
      901 tggtgcggac gccgcgcgac ccgtcccgac cgtcgcgacc tacaacgcct actccgccgg
      961 gctcgtcgga gaacacggcc tgcgcgtcgg cgtcgatccc gactccgtcg tcctcaccga
     1021 cgcctcgctc tggcagatgg cctcggcgat cgtcgagggg tgggcggacg acctcgacgc
     1081 ggactcagcg gtgagttcga tcatcggggc ggttcctcgc ctggcggcgc agttgtccga
     1141 ccacggcgcc agccccggcg accttgcccg cgtgtgcgag gcgatcgcgc agacggtcgg
     1201 aagcatcccc tctggcgccc tgcgaggcgg gggggccccg accaaggccc agcgcaccga
     1261 ggcggcgaag ttccgcgcgc gcgcggccat ggcaccgctg ctcgaggaat tcgcacggcg
     1321 caagcgtgac ggttcgttcc tcgacttcgc cgaccagatc gcgctcgccg ggaggctcgc
     1381 ccgcatcccc gccgtgcgcg cggtcgagcg ctcgcgctac cgcgtcgtgc ttcttgacga
     1441 gttccaggac acctcccacg gccagatgga cctcttcgcc gtgatgttcg gccagagtca
     1501 ccccgtcatg gcggtcgg
//
LOCUS       DASVKP010000057         3775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_347099, whole genome shotgun sequence.
ACCESSION   DASVKP010000057 DASVKP010000000
VERSION     DASVKP010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347099"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VF362_01175"
     CDS             complement(<1..207)
                     /locus_tag="VF362_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589572.1"
                     /translation="MAIIGRTTTDGPESRVLDGALTGEDYMSLVVSKDTSARAAAAAR
                     DDAPLGALLAFAQDPKVEVRVAVAS"
     gene            367..1773
                     /locus_tag="VF362_01180"
     CDS             367..1773
                     /locus_tag="VF362_01180"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX7589573.1"
                     /translation="MPDAPEFRTEHDTMGEVRVPKDALWGAQTQRALENFPVSGTVLE
                     RRHISALALVKKAAAVANLELGVLDADVADAIVSSAEEVARGDHDDHFPVDVFQTGSG
                     TSSNMNANEVIASLAAARLGREVHPNDHVNASQSSNDVFPTSVHVAAADGVVHDLVPA
                     LEHLAKALEAKAQEFASVVKAGRTHLMDATPVTLGQEFGGYAATVRLGIERLEAALPR
                     VAEVPLGGTAVGTGINTPAGFSSRVIALLAEYADLPLTEARNHFEAQSTRDGLVELSG
                     VLRTIAVGLTKICNDLRWMGSGPNTGLGEIRIPDLQPGSSIMPGKVNPVIPEAVLMVC
                     SRIVGNDATVAWAGASGAFELNVQIPVMALGTLESIRLLSTSARLLADKTVMGIEANV
                     ERARRNAEASPSIVTPLNRIVGYENAAKIAKHAVARGLTVREATIDLGFVERGEITEE
                     QLDALLDVTTMTGNAPPR"
     gene            complement(1820..3115)
                     /locus_tag="VF362_01185"
     CDS             complement(1820..3115)
                     /locus_tag="VF362_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX7589574.1"
                     /translation="MLDTSVLLSDPRASLRFAEHAVVLPVVVITELEAKRHHPELGYF
                     ARAALRLLDDLRVKHGHLDEPIEINEAGGTLRVELNHSDPDVLPSGFRLGDNDSRILA
                     VAANLRAEGHAVTLVSKDLPMRVKASALGMDAEEYRAELAVESGWTGMREIRLTDQQM
                     SALYEGERIAAPPEEDAGEDLAALPVHTGLVIHSTRGSALGRVTEGGGIRLVRGDLEV
                     FGIHGRSAEQRIALDLLLDESIGIVSMGGRAGTGKSALALCAGLEAVMERRQHKKIMV
                     FRPLYAVGGQELGYLPGSEAEKMNPWAQAVFDTLGAVVSKDVLDEVMARGMLEVLPLT
                     HIRGRSLHDAFVIVDEAQSLERNVLLTMLSRIGQGSRVILTHDVAQRDNLRVGRHDGV
                     AAVIEALKGHPLFAHVTLERSERSAVAALVTDILERLDL"
     gene            complement(3330..>3775)
                     /gene="uppS"
                     /locus_tag="VF362_01190"
     CDS             complement(3330..>3775)
                     /gene="uppS"
                     /locus_tag="VF362_01190"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX7589575.1"
                     /translation="GRWTLQVVGALELLPPQVAARLRSAGRQGRTEGGLHVNIAVGYG
                     GRREIADAVKAFLQEGVDHGRSLAEVAQDFSVEHIAQHLYTKGQPDPDLVIRTSGEQR
                     LSGFLMWQSAHSEFYFCDAYWPDFRHVDFIRALRAYSQRERRRGR"
BASE COUNT          630 a         1307 c         1273 g          565 t
ORIGIN      
        1 cgaggcaacg gcgacacgga cctcgacctt ggggtcctgc gcgaacgcga ggagggcacc
       61 caagggggcg tcgtctcggg cggcggccgc cgcgcgcgcc gacgtgtcct tcgacaccac
      121 caggctcatg tagtcctcgc ccgtcagggc cccatccagc acccgggact cgggtccatc
      181 agtggtcgtc ctaccgatga tcgccatctg gatgcgtctc ctagggtcgc gagggggcgc
      241 gagcaggaca tgcgcgcccc gccattgtgg ctgctacagg atgagaggga cgtcaccccc
      301 gatcgtgacg ccacttcccc ggacttcttc ggagagtcta ccggagcaac ctgacagaat
      361 ggggccatgc ccgacgcacc cgagttccgc accgaacacg acacgatggg cgaggtgcgc
      421 gtccccaagg atgcgctgtg gggggcgcag actcagcgcg cactcgagaa cttcccggtc
      481 tccgggaccg tgctcgagcg caggcacatc agcgcactcg ccctggtgaa gaaggcggcc
      541 gcggtcgcca acctcgaact cggagtactc gacgccgatg tcgccgatgc gattgtcagc
      601 tcggcggaag aagtcgcccg cggcgaccat gacgaccact tccccgtcga cgtcttccag
      661 accgggtccg gaacgtcgtc gaacatgaac gccaacgagg tcatcgcgag cctcgccgcc
      721 gctcgactcg ggcgcgaggt tcaccccaac gaccacgtga atgcctcgca gtcctcgaac
      781 gacgtctttc ccacgtccgt gcacgtcgcg gcggccgacg gcgtggtcca cgacctggtg
      841 cccgccctgg agcacctcgc caaggcgctc gaggcgaagg cgcaggagtt cgcttccgtc
      901 gtgaaggcgg gcagaacgca cctgatggac gcgacccccg tgacactcgg ccaggagttc
      961 ggcggatacg cggcgaccgt ccggctcgga atcgagcgcc tagaggcggc cctgccccga
     1021 gtcgccgagg tgccgctggg gggaaccgcc gtcggaacgg ggatcaacac gcccgccgga
     1081 ttctcgtcgc gggtcatcgc gctgctcgcg gagtatgccg acctgcccct gaccgaggcg
     1141 cggaatcact tcgaggccca gtccacgcgg gacggccttg tggaactcag cggcgtgctg
     1201 cggaccatcg ccgtcggcct caccaaaatc tgcaacgacc ttcggtggat gggatccggc
     1261 cccaacaccg gcctgggcga gatccgcatt cccgaccttc aacccggctc gtcgatcatg
     1321 ccgggcaagg tcaaccccgt cattccggag gccgtgctca tggtctgctc ccgcatcgtc
     1381 ggcaacgacg ccaccgtggc gtgggcgggg gcctctggcg cgttcgagct caatgttcag
     1441 attcccgtca tggcactcgg aacgctggag tccatacggc tcctcagcac ctccgcgcgc
     1501 ctgctcgcag acaagaccgt gatgggaatc gaggcgaacg tcgagcgggc caggcgcaac
     1561 gccgaggcgt cgccgagcat cgtcaccccg ctgaaccgca tcgtcggcta cgagaacgcg
     1621 gcgaagatcg ccaagcacgc cgtcgcgcgc ggcctcacgg tgcgcgaggc gacgatcgac
     1681 ctgggcttcg tcgagcgggg cgagatcacc gaggagcaac tcgatgccct cctcgacgtg
     1741 acgaccatga ccgggaacgc accaccgcgc tgacccagac ggcggctccc ccgcgtggtg
     1801 tccgacgcgg cgacccaaac tagaggtcca ggcgctccaa gatatcggtc acgagtgccg
     1861 cgaccgccga gcgttcggac cgctcgaggg ttacatgagc gaagagcggg tgacccttca
     1921 gcgcctcgat cacggcggcc actccatcgt ggcgtcccac gcgcaggttg tcgcgctgag
     1981 cgacatcgtg ggtaaggatc acgcgggaac cctgtccgat ccggcttagc atcgtcagga
     2041 gcacgttgcg ctcgagcgac tgggcctcat cgacgatcac aaacgcgtcg tggagggaac
     2101 ggccgcgaat gtgcgtcagc ggcagcacct cgagcatccc gcgggccata acctcgtcca
     2161 gcacgtcctt ggacaccacc gcgccgaggg tgtcgaagac ggcctgcgcc catgggttca
     2221 tcttctctgc ctcgctaccg ggcaggtacc cgagctcctg tcccccgacg gcgtagaggg
     2281 gccggaagac catgatcttc ttgtgttggc ggcgctccat gaccgcctca agccccgcgc
     2341 acaaggcgag ggcgctcttg cccgttccgg cgcgtccccc catcgagacg atcccgatgc
     2401 tctcgtcgag gagcaggtca agggcgatgc gctgctcggc gctccggccg tgaatcccga
     2461 aaacctcaag gtcgccgcga acaagccgga tcccgccgcc ttcggtcacc cttccgagcg
     2521 cggagccgcg agtcgagtgg atcacgagcc cggtatggac gggaagcgcc gcgaggtcct
     2581 cccccgcgtc ttcctccggg ggggccgcga tccgctcacc ctcgtagagg gcggacatct
     2641 gctgatccgt caggcgaatc tcccgcatgc cggtccatcc ggactcgacg gcaagttcgg
     2701 cgcggtactc ctcggcgtcc attcccagcg cggacgcctt gacgcgcatg gggaggtcct
     2761 tggacaccag ggtgacggca tgcccctcgg cgcgaagatt tgccgcgacc gcaaggatcc
     2821 gcgagtcgtt gtcgcccagc cgaaacccgc tggggagcac gtcggggtcg gagtggttga
     2881 gctcgacgcg cagggttcca cccgcctcgt tgatctcgat cggctcgtcc aggtgtccgt
     2941 gcttcacgcg gaggtcatcc aggagccgca gcgccgccct agcgaagtag ccgagttcgg
     3001 gatggtgcct cttcgcctcg agctccgtga tcaccacgac cgggaggacg acggcgtgct
     3061 cggcgaagcg cagcgaagcc ctgggatcgg aaagcaggac ggaggtatcg agcacgaaag
     3121 tgcgaaggcc cgtggcgagt ttgctcgggg cttcggcggg ggaagggctc tgcgtgacgg
     3181 tcacaagtgc tccctggctg ccggtcgagc cgcgggggcg ctccgccccg tggcggaggt
     3241 tgctatggtg ctaggtcggt acccaccatg attcgacggt acgcgcggcc gggcgtgtgc
     3301 ctcggcaaca cgccgggccg gaggggcgcc tagcgcccgc gacgacgctc gcgctgcgag
     3361 tacgcgcgaa gggccctgat gaagtccacg tggcggaagt ccggccagta cgcgtcgcag
     3421 aagtagaact cgctatgcgc gctctgccac atgaggaatc cactcaggcg ctgctcgccg
     3481 gacgtccgga tgacgaggtc ggggtcgggc tgacccttgg tgtaaaggtg ctgggcgatg
     3541 tgctccacgg agaagtcctg agcgacctcg gcgagcgagc ggccgtgatc cactccttcc
     3601 tggaggaacg ccttcaccgc gtcggcgatc tcgcgcctgc ccccgtagcc gacggcgatg
     3661 ttcacgtgga gcccgccctc ggtacggccc tggcggccgg ccgagcggag ccgcgccgcg
     3721 acctggggcg gcaggagttc gagcgccccc acaacctgga gcgtccagcg gccat
//
LOCUS       DASVKP010000058         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1415917, whole genome shotgun sequence.
ACCESSION   DASVKP010000058 DASVKP010000000
VERSION     DASVKP010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415917"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1325)
                     /locus_tag="VF362_01195"
     CDS             complement(87..1325)
                     /locus_tag="VF362_01195"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX7589576.1"
                     /translation="MKVYLALLVIAALVTYVATPVMRHLAYRVGAVTTARERDVHTVP
                     TARLGGVAIYLGLAAAIVTASRVPFLGTVFEVSGAAWAVLAGAGLVCLLGVIDDIYDL
                     DWMAKLAGQVFAAWILAWGGVQLVTVPIAGLTIASSPLSLAATMLIVVIAINAVNFVD
                     GLDGLAAGMIAIGASALLVYTYVLARDASPGDYSSLATLVLAALVGACLGFLPHNVHP
                     ARIFMGDSGAMVLGLTMAAAAIIVTGQVDPEIVKQRGALVPAFVPIILPIMVVAVPLA
                     DMVLAVIRRTMRGESPFAADAHHLHHRLLRFGHSHRWAVAVLYIWTAVLSFGTVSLVF
                     MGVRNAAILLGSGIVAAALITFSPKFRDALSLALRSSWTRAVEALGAAASKVRGRRSS
                     RHARVVTGEDSAEVVHGGKK"
     gene            complement(1322..>1845)
                     /locus_tag="VF362_01200"
     CDS             complement(1322..>1845)
                     /locus_tag="VF362_01200"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX7589577.1"
                     /translation="PEAVEAILAAKGRGREMPPPVLIPSAGTVDGIARDVPDHVRAIM
                     DAFWPGPLTIILNAQPSLSWDLGETGGTVALRVPRHPVALALLLRTGPMAVTSANRTG
                     GPPATSVEGAEWQLGSAVSVYLDGGETPIGLPSTIIMGTGDRIRIVREGALHRDELEA
                     VAGKGAVDGGSSG"
BASE COUNT          318 a          647 c          654 g          226 t
ORIGIN      
        1 tccgacgccg actcccgcta tggcgatgac gcccatcgcg atcgccaccc cgaccagggc
       61 cctccggtag accgcacgtt cctcgctcat ttcttccccc cgtggacgac ctctgcggaa
      121 tcctcgccgg tgacgacgcg cgcgtgccga gaggatcgac gccctcggac cttggaggcc
      181 gccgcgccaa gcgcttccac ggcgcgcgtc caggaggacc gtagcgccag cgagagcgca
      241 tcccggaact tgggcgagaa cgtgatcagc gcggcggcga cgatgccgct accgagaagg
      301 atggccgcgt tgcggacgcc catgaacacg agcgagaccg tcccaaagct cagcacggcg
      361 gtccagatgt agagcacggc gaccgcccat cggtgcgagt gcccgaagcg gaggagccgg
      421 tggtgaaggt gatgggcgtc cgccgcgaag ggagactcac cccgcatcgt cctgcggatg
      481 acggcaagga ccatgtccgc gaggggaacc gcgaccacca tgatgggaag gatgatgggc
      541 acgaaggcgg gcacgagcgc accccgctgc ttgacgatct ccggatcgac ctggcccgtc
      601 acgatgatcg cggcggcggc catcgtcagc ccgagcacca tcgcgcccga gtcgcccatg
      661 aagatgcgcg ccgggtggac gttgtgcgga aggaagccga gacacgcgcc gacgagcgcg
      721 gcgagcacga gcgtcgcgag gctcgagtag tcgccgggcg acgcgtccct cgcgaggacg
      781 taggtgtaca ccagaagggc ggaggcgccg atcgcgatca tgcccgccgc gaggccgtcg
      841 aggccgtcga cgaagttcac ggcgttgatc gcgatgacga cgatgagcat ggtcgccgcg
      901 agcgacagcg ggcttgaggc gatcgtgagg cccgcgatcg gaacggtcac gagttggacc
      961 ccgccccacg cgaggatcca ggccgcgaac acctgaccgg cgagtttcgc catccagtcg
     1021 aggtcgtaga tgtcgtcgat cacgccgagg agacacacga ggcccgcgcc cgcgaggacc
     1081 gcccacgccg cccccgaaac ctcgaagacc gtcccgagga agggaacgcg gctggcggtc
     1141 acgatggcgg ccgcgagccc caggtagatg gcgaccccgc cgagccgggc cgtcgggacg
     1201 gtgtggacgt cgcgttcgcg ggcggtggtc accgcaccga cgcggtacgc caggtgccgc
     1261 atcacgggcg tcgccacgta ggtgacgagc gcggcgatga ccaagagggc gaggtagacc
     1321 ttcacccgga ggaccccccg tcgaccgctc ccttgccagc gaccgcctcc agttcgtcgc
     1381 gatgaagcgc tccctcgcgc acgatgcgga tccgatcccc cgtccccatg atgatcgtcg
     1441 acggcaggcc gatcggcgtc tctccgccgt cgaggtagac cgacaccgcg gaacccagtt
     1501 gccactcggc gccctcgacc gaggtcgccg gaggtccgcc agtgcgattc gcgctcgtca
     1561 cagccatcgg gccggtgcgg agcaggagcg cgagcgcgac cgggtgccgc ggcacgcgca
     1621 gcgcgaccgt cccccccgtc tccccgaggt cccacgagag ggagggctgg gcgttgagga
     1681 tgatcgtgag gggcccgggc cagaaggcat ccataatcgc gcggacgtga tccggcacgt
     1741 cccgcgcgat tccgtcgacc gttcccgcgg acgggatgag cacggggggc ggcatctcgc
     1801 gcccgcgccc cttggcggcg aggatcgcct cgaccgcctc cggtc
//
LOCUS       DASVKP010000059         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1497516, whole genome shotgun sequence.
ACCESSION   DASVKP010000059 DASVKP010000000
VERSION     DASVKP010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497516"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..174)
                     /locus_tag="VF362_01205"
     tRNA            complement(88..174)
                     /locus_tag="VF362_01205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(138..140),aa:Leu,seq:cag)
     gene            222..929
                     /locus_tag="VF362_01210"
     CDS             222..929
                     /locus_tag="VF362_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEX7589578.1"
                     /translation="MGVLDRFERGVENAVNAAFSRTSRSGVKPIELAAALRKEADAKA
                     AAVDRARTVAPNEYDLLLSPADHEAVVAWGEDAMAREFEDALHEHAEYQRYTFVGSVT
                     VRFFLEEALSTGRFTVASRSSRSPATEASGASVNSRRPLIDVDGRRFHLTGESTTVGR
                     GTEADIVIEDTGVSRVHARFDVTEFGTILTDLGSTNGTFVEDLRIKEVTLLDGNVVTI
                     GRTTIMYWDALPLAGDV"
     gene            931..1449
                     /locus_tag="VF362_01215"
     CDS             931..1449
                     /locus_tag="VF362_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7589579.1"
                     /translation="MSQLSLTLLRLGFLALLWALVLSAIAVLRADIYGTRVFARGRGR
                     TNTRTMRAKTSASSPPARAATGVKAPPRGAGGEKLRLVVVAGPLTGTSIPLEQSTITI
                     GRSPRATLVIEDDYCSSRHARVFLEGDTWMVEDLGSTNGTFLNDAKLTEPEEFLLDSR
                     LTMGATSLEMRT"
     gene            1451..2662
                     /locus_tag="VF362_01220"
     CDS             1451..2662
                     /locus_tag="VF362_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX7589580.1"
                     /translation="MFLGLDFAARSDVGLVRSNNQDSAYAGPHLLVVADGMGGAAGGD
                     IASSIAVGTLVGLDGEALGPGEAIEALKEAIALAHTQIVERAHNDPELSGLGTTVTAI
                     LRAGNAIAMAHIGDSRAYLLRDGVFDQITSDHTFVQHLVDTGRLSAADAEHHPKRSML
                     LRVLGDVDADVPVDISARETKVGDRWLLCTDGLSSVVARATLAKTLGATVGPAECADR
                     LVALALAGGAPDNVTCIVADVVDVDRARGKAVPPDASQIVGSAAAGPNAAAAARELAK
                     DSPAARAAAGSEVAPDETPRRRVVQIWERIRPWSLPAAGLLVVVAAGFGGYAWTQNQY
                     YVAEHNGDVSIFRGIPQALGPFDLSSLVKDTGIKLSDLQPVEQDRIRSAIQADSLAAA
                     LKVVDDAVGTP"
     gene            2680..>3188
                     /locus_tag="VF362_01225"
     CDS             2680..>3188
                     /locus_tag="VF362_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX7589581.1"
                     /translation="MRIRSSRRAEGFLLAFAIAIPVSAYALVGLGATGGLPANVWFFG
                     LGTAALAIAGHGALRSWAPAADPVLFPAAFALNGVGLAMIYRLDLERAGTSSASHYAT
                     RQLAWSVIGVTFAVLVIVALRDHRMLRRFTYTAMVAGLACLLLPLLPVLGQERRGARL
                     WIEVFGMSF"
BASE COUNT          485 a         1115 c         1054 g          534 t
ORIGIN      
        1 ctagtctgcc ctagcgcggg acgcggcggc cacctcggcc cgcgccagcg acgtcgggcg
       61 ggggccccgg gacaacccca ggggcgatgg tgcgcgagag gggagttgaa cccctacgcc
      121 ctttcgggca cacggacctg aaccgtgcgc gtctgccaat tccgccactc gcgcgcgccg
      181 gacgactcta gcacgggagg cccggcggat acgatgccta catgggtgtg ctcgatcggt
      241 tcgagcgtgg cgtggagaat gcggtcaacg ccgccttctc cagaacgtcg cgctcgggag
      301 ttaagcccat cgaacttgcc gccgcgcttc gcaaggaggc ggacgccaag gcggcggccg
      361 tcgaccgtgc gcgaacggtc gcgccgaacg aatacgacct tctcctcagc cccgccgacc
      421 acgaggcggt cgtcgcgtgg ggcgaggatg cgatggcacg cgagttcgag gatgcgctcc
      481 acgagcacgc cgagtaccag cgatacacct tcgtcggttc ggtgaccgtg cgcttcttcc
      541 tcgaggaggc cctctcgact gggcggttca ccgtcgcgtc gcgctcgtcg cgcagcccgg
      601 ccacggaggc gtcgggcgcc tcggtcaact ctcggcgccc gctgatcgac gtcgacggac
      661 ggcgctttca cctcacaggg gagagcacga cggtcgggag gggcacagag gccgatatcg
      721 tcatcgagga caccggagtc agcagggtcc acgcgcggtt cgacgtcacc gagttcggga
      781 ccatcctcac cgatctcggc tccaccaacg gaacgttcgt cgaggacctg cgcatcaagg
      841 aagtcaccct gctcgacggc aacgtggtga cgatcggacg gaccaccatc atgtactggg
      901 atgcacttcc gctggcgggg gacgtgtgac gtgagccaac tctcgctcac ccttctccga
      961 ctcgggtttc tcgctttgct ctgggccctt gtcctctccg caatcgccgt tctccgcgcc
     1021 gacatttacg gcacgcgtgt cttcgcgcgc ggaaggggac ggacgaacac gaggacgatg
     1081 cgcgcgaaga cgtcggcctc gtcgccgccc gcgcgcgcgg caaccggagt gaaggcgcca
     1141 ccgcgaggag ccgggggaga gaagctccgg ctcgtcgtcg tcgccggccc gctcacgggc
     1201 acctcgattc ctctcgagca gtccaccatc accatcggtc ggtccccccg cgcgacgttg
     1261 gtcatcgaag acgactactg ctcctctcgc cacgcgcgcg tcttcctcga gggcgacacg
     1321 tggatggtgg aggatctcgg ttccaccaac ggaaccttcc tcaacgacgc gaagttgacg
     1381 gaaccggagg agttcctcct ggattcgcgc ttgaccatgg gtgcgacgtc gctcgagatg
     1441 aggacgtgac atgttcctag gactggactt tgcggcgcgg tcggacgtcg gcctcgtgcg
     1501 cagcaacaac caggactcgg catacgcggg accccacctt ctcgtggtcg ccgacggcat
     1561 gggcggagcc gcggggggcg atatcgcctc ctcgatcgcg gtcggaacgc tcgtgggcct
     1621 tgacggcgaa gccctcgggc cgggggaagc catcgaggcg ctcaaggagg ccatcgccct
     1681 tgcgcacact cagatcgtcg aacgcgctca caacgatccc gaactcagcg gacttggcac
     1741 aacggtcacc gcgattctgc gggccgggaa cgccatcgcc atggcgcaca ttggtgactc
     1801 ccgcgcgtac ctccttcgcg acggcgtctt cgaccagatc acgagcgacc acacttttgt
     1861 tcagcatttg gtcgacacgg gtcgcttgag cgccgcggac gccgagcacc accccaagcg
     1921 ttcaatgctt ctgcgcgtcc tgggcgacgt cgacgccgac gtgcccgtgg acatctcggc
     1981 tcgcgagacc aaggtgggcg atcggtggct cctgtgcacg gatggcctga gttccgtggt
     2041 ggcgcgggcc accctcgcga agacgctcgg ggcgacggtc ggcccggcgg aatgcgccga
     2101 tcgcctcgtc gcgctcgcac tcgccggcgg cgcccccgac aacgtcacgt gcatcgtcgc
     2161 cgacgtcgtt gacgtcgatc gcgcccgcgg aaaggcggtc ccgccggacg cttcgcaaat
     2221 tgtcggctcg gcggcggccg gtcccaacgc cgcggccgcc gcgcgagagc tggccaaaga
     2281 ctcgcctgcc gcccgtgctg cagccgggtc cgaggtcgcc cccgacgaga ctccccgccg
     2341 ccgcgtcgtg caaatctggg aacgtattcg tccatggagt cttcccgccg ctggcctcct
     2401 cgtggtggtc gcggcggggt tcgggggcta cgcatggacc cagaaccagt actacgtcgc
     2461 cgaacacaac ggtgacgtga gcatcttccg cgggattccc caggccctcg gcccgttcga
     2521 tctgtcctca ctcgtgaagg acacgggaat caagctgagc gatcttcaac ccgtcgagca
     2581 ggatcgaatt cgctccgcga tccaagcgga ctcgcttgcc gccgcattga aagtggtcga
     2641 cgacgcggtg ggcaccccct agtggcgact gtcgccgacg tgcggattcg gagcagtcgc
     2701 cgcgccgagg gattcctctt ggcgttcgcg atcgcgatcc ccgtttcggc gtatgcgctc
     2761 gtcggccttg gggccacggg cgggctcccg gcgaacgtct ggttcttcgg cctcggtacg
     2821 gcggccctcg cgatcgcggg gcacggcgcc cttcggtcgt gggcgcccgc cgcggatccg
     2881 gttctgttcc ccgcggcatt cgccctcaac ggcgtggggc tggccatgat ctaccgcctc
     2941 gatcttgagc gcgcgggaac ctcgagcgcg tcccactacg cgacgcgcca actcgcctgg
     3001 agcgtgatcg gcgtgacttt cgcggtactc gtcatcgtcg ctctacgcga ccaccgcatg
     3061 ctccggcgct tcacctacac ggcgatggtc gccggactcg cctgtctcct ccttcccctg
     3121 ctcccggttc tcgggcaaga gcgccgcggc gctcggctct ggatcgaggt gttcgggatg
     3181 tcgttcca
//
LOCUS       DASVKP010000060         5436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_677423, whole genome shotgun sequence.
ACCESSION   DASVKP010000060 DASVKP010000000
VERSION     DASVKP010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677423"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1332
                     /locus_tag="VF362_01230"
     CDS             106..1332
                     /locus_tag="VF362_01230"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX7589582.1"
                     /translation="MTATSVATAQGFTVAPASPTLRTRVWRGRFWWSLVLVFSALLAV
                     VVASTPSKDSALLSLNNPGSDGARATGEILRGRGLDVRQITRLGAARIATPQDTTLAV
                     VLPSNIVDYQIESVLAYPGDIVFVGVSPDLLTAIDPSLGSADSPESTLRGAQCNDPDA
                     IAAGRISTGGAEIDATRVDGATVCFRSAPGIGPFVVLQRAGRTITLLSDATLPMNGNL
                     IDYGNAALVFRALGKHPTLVWYLGNPLDTSTLTYSDGSGTGSNPGGNAAEPSADFLPP
                     GTGTALYALAIAVLIAAIWRGRRMGPLVTEALPVVVPSSETTRGRARLYRRARAYGRA
                     SASLRAAAAERMGRRLGVPRGGDAATLIRAVARATGREPLAVERLVYGPPPDGEHAMM
                     DLVQELDTLERQVHRP"
     gene            1329..2327
                     /locus_tag="VF362_01235"
     CDS             1329..2327
                     /locus_tag="VF362_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX7589583.1"
                     /translation="MTETPTPSAPTPADAPRSAARESLAKIRTEVGKAVVGQDAAVTG
                     LVIALLCRGHVLLEGVPGVAKTLLVRALAASLGMDSKRVQFTPDLMPGDVTGSLIYDA
                     RTAAFSFRQGPVFTNMLLADEINRTPPKTQASLLEAMEERQVTIDGQSHALPDPFLVI
                     ATQNPIEYEGTYPLPEAQLDRFMLKLLMPLPERDVETEVVRRHATGFNPRDLRAAGLR
                     PVATPADLEKGRREVAAVEVSEDVMGYIVDLCRATRAAPSVSLGVSPRGATALLATSR
                     AWAWMRGRTFVSPDDVKSLAVPTLGHRIQLRAEAELEGVTTAAVLDSVLASVPVPK"
     gene            2331..3623
                     /locus_tag="VF362_01240"
     CDS             2331..3623
                     /locus_tag="VF362_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7589584.1"
                     /translation="MYITGWTVLLAALGAVPAALTQSRGVAVLWALGTLALAGIDALL
                     APPVTSIGVQRKVPRSVRLTESTASVVTVANLTTRRIRGSLRDAWPPSAGARANRHRM
                     RIPAHQIQKFTTPLQPTRRGDRHSDVVTVRLEGPLHLAGRQRAIDARDVLRVLPEFRS
                     RKHLATRLARLREIDGQTSVNLRGAGSEFDSLREYVVGDDVRTIDWRGTARRADVVVR
                     TYRPERDRRVFILVDTSRLSAARVGDAPRLDASIEAALLLAALASRAGDRVQVVAFDR
                     RERSRAMGTTSAIVMPALADALATAEPELVEPDWPGALRLIDERLSQRAMVLLLTTID
                     ASTVDNGLLEAVGALARLHQVVVASVDDPDVAALANGRGDASAVFGAAAAERSRLEAS
                     AVAMRIRQLGAEVVRSVPDALAPDVADKYLELKAAGRL"
     gene            complement(3691..4686)
                     /locus_tag="VF362_01245"
     CDS             complement(3691..4686)
                     /locus_tag="VF362_01245"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX7589585.1"
                     /translation="MDIDAFSAIRAARWARLRELSGSRRLTGAEADEFTRLYQMTAGD
                     LGAVRSAAPEPGLITWLSVQLANARVWLTGAHEFSLGDVRHVFSRTFPAALYRVRWWS
                     VGVAGVVIAGGILSAFYALHTPGALDLVGPPEVRAQIAQEEFSSYYTQYDSTSFGAQV
                     WTNNALLSTLAIGTGIVGYLPAVLMYNTALQLGVNAAIMSEAGMLDVFFKLIIPHGLL
                     ELSAVFTAAGAGLRLFWTALVPGARTRSRALAEEGRIAIAVAGVLVVTLFASGLIEGY
                     VTGSAMAWWLKIAIGAVAFAAFWVYTFVVGRLVTREGATGDAEGDFAVATAPVAG"
     gene            4726..>5436
                     /locus_tag="VF362_01250"
     CDS             4726..>5436
                     /locus_tag="VF362_01250"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX7589586.1"
                     /translation="MSEFEDEILTGEGVLLEASAAPVTYRIASGSIDAVATVIVLWLS
                     ATLVGALASSLNAAAGTAIVIMYIVSLLVLAPATVETLTRGRSLGRMALGLRIVRDDG
                     GPVSFRQAITRSIVALLEVWVSFGMIAVTTSLLSARGKRLGDMLAGTYALRTRGTMKP
                     LPPVLLPPALAEWSRTADMSRLPDGLALYARLFLGRAAAMHPAARQRLGTRYAERLGE
                     HVAPPPPFGTHPETYIAAV"
BASE COUNT          784 a         2066 c         1805 g          781 t
ORIGIN      
        1 atcgggaggc gacgtcccag gactacgaca ccatgacgtc gcttgatcgc tcgctcgctg
       61 gccgctccat cctcacggcg ggcgtctcgt gatcgcgggc gtcccgtgac ggcgacgtcc
      121 gtcgcgacgg ctcagggctt caccgtcgcc cccgcgtctc ccacactccg cacgcgcgtg
      181 tggcgcggcc gcttttggtg gagcctcgtg ctcgtcttct cggcgctcct tgccgtggtg
      241 gtggcgtcga ccccgagcaa ggactccgca ctcctctccc tgaacaaccc cggttccgac
      301 ggcgcgcgcg ccacgggcga gatcctccgg ggccgcgggc tcgacgtacg gcaaatcacc
      361 aggcttggcg ccgcgcggat cgcgacgccg caggacacca cgctcgccgt ggttctgccg
      421 agcaacatag tcgactacca aatcgagtcc gtgttggcat acccgggcga catcgtgttc
      481 gtcggcgtca gccccgacct cctcaccgcc atcgacccct cgctcgggtc ggcggattcg
      541 ccggagtcga cccttcgtgg ggctcagtgc aacgatcccg atgcgattgc ggcgggacgc
      601 atctccacgg gcggagccga gatcgacgcg acgagggtgg acggcgccac ggtgtgcttt
      661 cgtagcgccc cgggaatcgg gccgttcgtc gtcctgcagc gcgcaggccg caccatcacc
      721 ctcctctccg acgcgacgct tcccatgaac gggaacctca tcgactacgg caacgccgcc
      781 ctcgtgttcc gcgcgctcgg caagcacccg acgctcgtgt ggtacctcgg caacccgctc
      841 gacacctcga cgctcacgta ctcggacggc agcggcacgg gatcgaatcc cggcgggaac
      901 gcggccgagc ccagcgccga cttccttccc cccggcacgg ggaccgcgct ctacgcgctc
      961 gcgatcgccg tgctcatcgc cgcgatctgg cgcgggaggc gcatggggcc ccttgtcacc
     1021 gaggccctgc ccgtcgtcgt gccctcctcg gagacgacga ggggccgagc ccgcctctac
     1081 cggagggccc gcgcctacgg tcgagcgtcc gcctctcttc gcgcggccgc ggccgagcgc
     1141 atgggccgtc ggctgggagt cccgcgcggc ggcgatgccg cgaccctcat ccgcgcggtc
     1201 gcccgagcca cgggacgtga gcccctggcc gtcgagcgcc tcgtgtatgg cccgccgccc
     1261 gacggcgaac acgcaatgat ggatctcgtt caggaactcg acacgctgga aaggcaggtt
     1321 caccgaccgt gacggagact cccaccccct cggcccccac gccggcagac gcgccccgct
     1381 cggcggcgcg cgaatcgctc gccaagattc gcacggaggt gggcaaggcc gtggtcgggc
     1441 aggatgcggc cgtcaccggc ctcgtcatcg ccctactctg ccgcggacac gtgctcctcg
     1501 agggcgttcc cggcgtcgcc aagacgctcc tggtccgggc cctcgcggcc tcgctcggga
     1561 tggactccaa gcgggtccag ttcacgcccg acctgatgcc gggcgacgtg acgggctcgc
     1621 tcatctacga tgcgcgcacc gcggccttct ccttccgcca ggggcccgtc ttcaccaaca
     1681 tgctgctcgc cgacgagatc aaccgaacgc cccccaagac ccaggcgtcg ctcctcgaag
     1741 cgatggagga gcgccaggtc accatcgacg gacaatccca cgccctgccc gatccgttcc
     1801 tggtcatcgc gacgcagaac ccgatcgagt atgagggcac ctacccgctt cccgaggcgc
     1861 aactcgaccg ctttatgctc aaactcctca tgccgctgcc cgagcgcgac gtggagaccg
     1921 aggtggtgcg tcgccacgcc acgggcttca atccgcgcga ccttagggcc gctgggctga
     1981 ggccggtcgc gacgccagcc gacctggaga aggggcggcg cgaggtcgcg gcggtcgagg
     2041 tatccgagga cgtcatggga tacatcgtcg acctctgccg tgcgacgcga gccgccccgt
     2101 ccgtctcgct tggcgtgtct ccgcgcggtg ccaccgcgct gctcgcgacg tcccgcgcat
     2161 gggcgtggat gcggggccgc accttcgtct cgccggatga cgtcaagtcg ctggccgtgc
     2221 cgaccctcgg gcaccggatc cagctgcgcg ccgaggccga actcgagggc gtgaccaccg
     2281 cggccgtcct cgactccgtc ctcgcgtcgg tcccggtccc gaagtagctc atgtacatca
     2341 ccgggtggac cgtcctcctc gccgccctcg gcgcggtccc ggcggccctc acccagagcc
     2401 gcggcgtggc cgtgctctgg gccctcggaa ccctggccct cgcgggcatc gacgcgctcc
     2461 tcgcgcctcc cgtcacgagc atcggcgtcc aaaggaaggt tccgcgatcg gtccgcctca
     2521 cggagtccac cgcgtcggtc gtcacggtcg ccaacctgac gaccaggcgg atcaggggat
     2581 ccctgcgcga cgcctggccc cccagcgccg gcgcccgcgc gaaccgtcac cggatgcgca
     2641 ttccggcgca tcagatacag aagttcacca cgccgctcca gccgaccagg cgcggcgacc
     2701 gccactccga cgtcgtgacg gtcaggctcg aggggcccct ccacctcgcg gggaggcaga
     2761 gggcgatcga cgcccgcgac gtcctgcgcg tccttcccga gttccgttcg cgcaagcacc
     2821 tcgcgacgcg gctggcacgc ctgcgcgaga tcgacggcca gacctcggtc aacttgcgcg
     2881 gcgcgggaag cgagttcgac tcgctccgcg agtacgtcgt cggggacgac gtgcggacga
     2941 tcgactggcg cggcacggcg aggcgtgccg acgtcgtcgt gcggacctat cgacccgagc
     3001 gcgacagaag ggtcttcatc cttgtggaca cctcacgcct gtccgccgcg cgggtgggcg
     3061 acgccccccg actcgacgcc tcgatcgagg ccgccctcct gctcgcggcg ctcgcctcgc
     3121 gcgcgggtga ccgcgtccag gtggtcgcgt ttgaccgccg cgagcgctcg cgggcaatgg
     3181 ggacgacgtc cgcgatcgtc atgccagccc tcgcggacgc cctcgcgacg gccgagcccg
     3241 aactcgtcga gccggactgg cccggcgccc ttcggctgat cgacgagcgc ctctcccagc
     3301 gcgccatggt cctcctcctc acgacgatcg acgcctcgac cgtcgacaac gggctactcg
     3361 aggccgtcgg agcactcgcc cgcctgcacc aggtcgtggt cgcgagcgtg gatgaccccg
     3421 acgtcgcggc gctggccaac ggccgcggcg acgcatcggc ggtcttcggc gccgcggcgg
     3481 cggagaggtc tcgcctcgag gcgtcggccg tcgcgatgcg gatccgccag ctcggcgccg
     3541 aggtggtccg gtccgttccg gacgccctcg cacccgacgt cgcggacaag taccttgaac
     3601 tcaaggcggc ggggcggctc tagcgggacg accccgacgg cgcggtcgca accccgcagc
     3661 cggctcgagg actcggggcg gtagcctccc ctatcccgcg acgggggcgg ttgcgacggc
     3721 gaagtcgccc tcggcgtcgc ccgtcgcccc ctcgcgcgtc accagcctgc cgacgacgaa
     3781 cgtgtagacc cagaacgcgg cgaacgcgac cgccccgatc gcgatcttga gccaccacgc
     3841 catggcgctt cccgtgacgt aaccctcgat gaggcccgac gcgaagagcg tgacgacgag
     3901 cacgccagcg acggcgatcg cgatgcgccc ctcctccgcg agcgcccggg atctggttct
     3961 cgctccgggc acgagggcgg tccagaacag gcggagtccc gcccccgcgg cggtgaagac
     4021 cgcgctgagt tcgaggagcc cgtgggggat gatcagcttg aagaagacgt cgagcatccc
     4081 ggcctcggac atgatcgcgg cgttgacgcc caactgaagc gcagtgttgt acatcaggac
     4141 cgcgggcagg tagcccacga tgccggtccc gatcgcgagc gtgctcagca gggcgttgtt
     4201 cgtccacacc tgggcgccga acgacgtcga atcgtactgg gtgtagtagc tcgagaactc
     4261 ctcctgcgcg atctgcgcgc gaacctccgg ggggcctacg aggtcgagag cccccggggt
     4321 gtgaagcgca tagaacgccg acaggatgcc gcccgcgatg acgacgcccg cgaccccgac
     4381 cgaccaccac ctcacccggt acagggccgc ggggaaggtg cggctgaaga cgtgcctgac
     4441 gtcgccgagg gagaactcat gggcgcccgt gagccacacc cgcgcgttcg cgagttgcac
     4501 ggacagccag gtgatgagtc ccggctcggg cgcggcggac cgcacggccc caaggtcgcc
     4561 cgcggtcatc tggtagaggc gcgtgaactc gtccgcctca gcccccgtga ggcggcgcga
     4621 tccggagagt tcgcgcagcc gagcccaacg cgccgcccgg atcgcgctga acgcgtcgat
     4681 gtccatgcgg ctacgatctc acacagggat cgcgacggag ggctgatgag cgaattcgag
     4741 gacgagatcc tcaccggcga gggcgtcctg cttgaggcca gcgccgcccc ggtgacctac
     4801 cgcatcgcct ccggctcgat cgacgcggtc gccacggtca tcgtcctgtg gctgagcgct
     4861 acgctcgtcg gcgccctcgc cagctcgctg aacgccgccg cgggcacggc catcgtcatt
     4921 atgtacatcg tctccctgct ggtgctggca cccgcgacgg tcgagacgct cacgcgcgga
     4981 agaagcctcg ggcggatggc gcttgggctc agaatcgtcc gcgacgacgg cggacccgtg
     5041 tcgttccgtc aggcgatcac gcgctccatc gtggccctcc tcgaggtctg ggtgagtttc
     5101 gggatgatcg cggtaaccac ctccctcctc tcggcccgcg ggaagcgcct gggagacatg
     5161 ctcgcgggaa cctacgcgct ccgcacccgc ggcacgatga agccactccc ccccgtcctc
     5221 ttgccccccg cgctcgccga gtggtcccgc accgcggaca tgagtcggct ccccgatggg
     5281 ctcgcgctct acgcccgcct cttcctcgga cgcgccgccg cgatgcatcc cgcggcacgg
     5341 caacgcctcg gcactcgcta cgccgagcgg ctcggcgagc acgtggcgcc gccgccgccc
     5401 tttgggaccc accccgagac gtacatcgcc gccgtg
//
LOCUS       DASVKP010000061         2219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_759136, whole genome shotgun sequence.
ACCESSION   DASVKP010000061 DASVKP010000000
VERSION     DASVKP010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759136"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1108)
                     /gene="pheA"
                     /locus_tag="VF362_01255"
     CDS             complement(152..1108)
                     /gene="pheA"
                     /locus_tag="VF362_01255"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX7589587.1"
                     /translation="MTKPRYAYLGPQGTFTEAALRAMISPDQAEYLPQVDVLTALGAV
                     RSGAADYAVVAIENTVEGGVTATLDALAEGAPLVILGETVIPIEFELVARPGTTTADI
                     ALIATHPHGWAQCRRWLNANLPGVAHVTASSNTSALAGLADSSRSLADLGYDAALAPP
                     GAGAAHELAVLTRGIADNARAMTRFVKVGRPEAVPAPTGADKTTLVVHLPEDRAGSLL
                     EMLEQFAARGINLSRIESRPIGDRPGQYSFSIDAMAHVAEERMAEALIGLRRTCRLVI
                     FLGSYPSVTATGPTPLADGTRDDDFRSALAWVEELRKGKGAP"
     gene            complement(1105..1926)
                     /locus_tag="VF362_01260"
     CDS             complement(1105..1926)
                     /locus_tag="VF362_01260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7589588.1"
                     /translation="MAAKRASSAPSDEPVPGQGERASARVAAIIVAFNQERRVAATVR
                     GALSIPAVDLVLVIDDGSTDQTQSAAREAGAVVVRHSHRRGRSAAVETGFAVVTMRDE
                     PGAAPRHILLLDGGLGEHAVGAAPLVSAVTEGAADLAIAVVGGQGDRATRMASALARG
                     AIARASGWSPTEPLSRTRCLTRGAMEASIPLSRGAGLDVGMTLDVLLAGLAATEVECE
                     IRVRPAPAGARAPLLHANQYRDVMVAVSTRRIRRGFEATQDAVDPRGLFHREDDE"
     gene            2017..>2219
                     /locus_tag="VF362_01265"
     CDS             2017..>2219
                     /locus_tag="VF362_01265"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882228.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HEX7589589.1"
                     /translation="MHDRAGTVALPQDLVDVDALLGAYYDLIPDPGIPEQRVAFGTSG
                     HRGSSLDRAFNEAHIVAITGAIV"
BASE COUNT          323 a          782 c          802 g          312 t
ORIGIN      
        1 gacggatcga tcacacccga ggcacctccg tggacgtcac catggaggcg ccccagaggg
       61 ttcaggccga cggagagtcg ctcgggcgtg cgatctcgct gcgggccgtc gtcgacgcgg
      121 ggtgcctgac cgtgcggact ctcgggctgc cctagggcgc gcccttgccc ttccgcaatt
      181 cctcgaccca ggcgagcgcg gaacggaagt cgtcgtctcg cgtcccatcg gcaagcggcg
      241 tgggacccgt ggccgtcacc gacggatagg agccaaggaa gatcacgagc ctgcaggtcc
      301 gcctgagtcc gatcagcgcc tcggccatcc gctcctcggc gacgtgggcc atcgcgtcga
      361 tcgagaagga gtactgaccc gggcggtcgc cgatggggcg cgattcgatg cgggacaagt
      421 tgatgccgcg cgcggcgaac tgctcgagca tctcgagaag agatcccgcg cgatcctcgg
      481 gcaggtggac gacgagggtc gtcttgtccg cccccgtcgg cgctggcacc gcctccggac
      541 gccccacctt cacgaaccgc gtcatggcgc gggcgttgtc cgcgatgcct cgggtgagga
      601 ccgcgagttc gtgcgccgcg cccgcccccg gcggcgcaag ggcggcgtcg tatccgaggt
      661 cagcgagcga ccgcgaggag tcggcgaggc ccgcgagcgc ggaggtgttg gacgacgccg
      721 tcacgtgggc gacgcccgga aggttcgcgt tgagccatct ccggcattgc gcccagccgt
      781 gggggtgcgt cgcgatcagg gcgatgtccg ccgtcgtcgt gccggggcgc gcgacgagtt
      841 cgaactcgat ggggatcacc gtctcgccaa ggatgacgag cggggcgccc tcggccagcg
      901 cgtcgagggt ggcggtgacg ccgccctcga ccgtgttctc gatcgcgaca acggcgtagt
      961 cggccgcgcc ggaacgaacg gccccgagcg cggtcagcac gtccacctgg ggcaggtact
     1021 ccgcctgatc gggggaaatc atcgcgcgca gagccgcctc ggtgaacgtg ccctgcgggc
     1081 caaggtaggc gtagcgcggc ttggtcactc gtcgtcctct cggtggaaga gcccgcgggg
     1141 gtcgaccgcg tcctgcgtcg cctcgaaccc gcgccggatg cgccgcgtgc tgacggcgac
     1201 catgacgtca cggtactggt tcgcgtggag gagcggggcg cgggctcccg ccggagcggg
     1261 cctcacgcgg atctcgcact ccacctctgt ggcggccagc cccgccagga gaacgtcgag
     1321 cgtcatgcca acgtcgagcc ccgcgccgcg cgaaagcggg atcgaagcct ccatcgcccc
     1381 gcgcgtcagg cagcgcgtgc ggctcagcgg ttcggtcggg gaccaaccgg atgcgcgcgc
     1441 gatcgctccc cgggcgagcg cggatgccat cctcgtcgcg cgatcgcctt gtccacccac
     1501 aacggcgatc gcgagatcgg ccgctccctc ggtgaccgcc gacacgaggg gtgcggcgcc
     1561 caccgcgtgc tcgccgaggc cgccgtccag aagaagaatg tggcgagggg cggcgcctgg
     1621 ctcgtcacgc atggtcacga cggcgaagcc ggtttccacg gcggccgacc tcccgcgccg
     1681 atgcgagtga cgcactacga ccgcgcccgc ctcgcgcgcg gcggattggg tctggtccgt
     1741 cgagccatcg tcgatcacga gcaccagatc gacggcgggg atcgacagcg ctcccctcac
     1801 ggtcgccgcg acgcgacgtt cctggttgaa ggcaacaatg atcgcggcaa cccgcgcgct
     1861 ggcccgctcc ccttgacccg ggacgggctc gtccgacggt gcgctcgacg ctcgcttggc
     1921 ggccacaccc tcagcctacc gagagcggga aggggtcgcc gcacgccccg cgaacgggcg
     1981 gtggccgagc ggggtcggag gtgcgagagt gggcgcatgc acgatcgcgc cggaactgtc
     2041 gcccttcccc aggacctcgt tgacgtcgac gccttgctcg gcgcctacta cgacctcatt
     2101 ccggacccgg ggattccgga gcagcgcgtg gcgttcggaa cgtccggtca ccgcggatcg
     2161 tcgctcgatc gtgcgttcaa cgaggcgcac atcgtcgcta tcaccggagc gatcgtcga
//
LOCUS       DASVKP010000062         2588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1170300, whole genome shotgun sequence.
ACCESSION   DASVKP010000062 DASVKP010000000
VERSION     DASVKP010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170300"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..486)
                     /locus_tag="VF362_01270"
     CDS             complement(157..486)
                     /locus_tag="VF362_01270"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEX7589590.1"
                     /translation="MAELTSDERTQGMLAHLLNIFFPAVGPLVIWLIGKDKSAFVDTE
                     GKEALNWGITLVAGWIVGGILLVVLIGILILMAVGIAALVFGIMGAIAANKGQNYRYP
                     FTFRFIK"
     gene            complement(479..835)
                     /locus_tag="VF362_01275"
     CDS             complement(479..835)
                     /locus_tag="VF362_01275"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEX7589591.1"
                     /translation="MRYDNEGSSDTLAAEIAQVGAIFGPLIPWLVWWRRRGTNLFAER
                     EAATATNFGALVLVAFAIATAIRAFVPFLGFLGTVGQLAVVASSVVLCLQAYSAVRRG
                     VSATYPLEIEVVKSHG"
     gene            946..1959
                     /gene="hrcA"
                     /locus_tag="VF362_01280"
     CDS             946..1959
                     /gene="hrcA"
                     /locus_tag="VF362_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX7589592.1"
                     /translation="MLEERRCAVLHAIVEGYVATSEPVGSRALATHHHLGVSPATIRN
                     DMAALEEEGLIAQPHTSAGRIPTDKGYRYFVDHLAATALPEPQRRAITTFLSDAVDLS
                     DTVERSVKLLSQLTRQVAVIQYPSLREAKVRRVEIVRMGQERALVVVVSADARVEQST
                     IVLGEEPTDDAFERVAREVNEATQGLGPAAAERRLAEWAARPQQVPWAGVLADAIVGA
                     ARGGTVERVVFSGTQNLARADAGLEQDAIGSVLEAIEEQVVLLRLLHEMARDSTDVAV
                     RIGGETHHEALSKTSIVAAGYGPPGGESSLLGALGPTRMDYPGTMAAVRAVARYLSRV
                     VEM"
     gene            1956..>2588
                     /locus_tag="VF362_01285"
     CDS             1956..>2588
                     /locus_tag="VF362_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX7589593.1"
                     /translation="MTDYYEVLGVPRGATQDQIKRAYRALAREHHPDTGDGSDEERFK
                     AVSRAYEVLSTPEKRARYDAGVDPSGRGGAAGFGFEDILQTFFGGGGGARRGPASRTQ
                     RGGDTLIHAEVSLEEATFGVARDVTLSLADPCATCLGTCCAPGTSPKTCPSCGGTGVV
                     QRVARSLLGQVMSTGPCPACGGYGTTIEAPCTECSGQGRTNSRHTITVDIP"
BASE COUNT          424 a          861 c          917 g          386 t
ORIGIN      
        1 ggggtgatca tcccggggat cggctcgggg gccttcttct tcgcccgcga cgcgggggac
       61 ttcggggttt cggtcatggc ccgatcgtgc cacgacgcac aagggccccg catccatccg
      121 gggaaggatg cggggccctg atgttacgtc agggtgctac ttgatgaagc ggaaggtgaa
      181 cgggtagcgg tagttttggc ccttgttcgc ggcaatcgct cccatgatcc cgaacacgag
      241 cgcggcgatg ccgacggcca tgaggatcag gatgccgatg aggacgacga gaaggatccc
      301 gccaacgatc catcccgcga ccagcgtgat gccccagttg agggcttcct tgccctcggt
      361 gtcgacgaac gcgctcttgt ccttgccgat gagccagatg acgagcgggc cgactgcggg
      421 gaagaagata ttgagaaggt gcgccagcat tccctgcgtg cgctcgtcgg acgtaagttc
      481 agccatgtga tttcaccact tcaatctcga ggggataggt agctgacact ccccgccgta
      541 ccgcgctgta ggcctggagg cacaacacca cggatgaggc gacgactgcc aactgtccta
      601 ctgtaccgag aaaccccaag aagggaacga aggccctgat cgcagttgcg atggcgaacg
      661 cgaccaggac gagggccccg aagttcgtcg ccgtggcggc ctcccgctcg gcgaagaggt
      721 tcgttccccg tcgtcgccac caaacgagcc aggggatgag gggaccgaag atcgcaccta
      781 cctgggcgat ctccgccgcg agcgtgtcgg atgagccttc gttgtcgtat cgcatcgatc
      841 ctccgcgccc acggtaccgt ttcgcgggcg ggtccgggtg gcgcgatacg attagcactc
      901 acgccagggg agtgccagcg gtgagagcca gcgaggaggt gccgcgtgct tgaggaacga
      961 cgctgcgccg tgctccacgc aatcgtggag ggctacgtcg cgacgagcga acccgtgggc
     1021 tcccgcgcgc tcgcgacgca ccaccacctc ggggtttccc ctgccacgat ccgcaacgac
     1081 atggcggcgc tggaggagga ggggctgatc gcccagccac acacctcggc cggtcgcatc
     1141 ccgaccgaca aggggtaccg ctacttcgtg gaccacctcg cggccaccgc tcttcccgag
     1201 ccgcaacgcc gggccatcac gacgttcctc agcgacgccg tcgacctcag cgacacggtg
     1261 gagcgctcgg tcaaactcct cagccagctg acgcgccagg ttgccgtgat ccagtacccg
     1321 tcgctgcgcg aggcgaaggt gcgcagggtc gagatcgtga ggatggggca ggagcgcgcg
     1381 ctcgtcgtgg tcgtttcggc cgacgccagg gtcgaacaga gcaccatcgt cctgggcgag
     1441 gagccgacgg acgacgcgtt cgagcgcgtg gcgcgcgagg tcaacgaggc aacgcagggg
     1501 ctgggtccgg ccgcggcgga acgcaggctc gccgagtggg ccgcgcggcc gcagcaggtt
     1561 ccgtgggcgg gggttctcgc cgacgccata gtcggggccg cccgcggcgg gacggtggag
     1621 cgggtcgtgt tctccgggac acagaatctt gcgcgcgccg atgctgggct cgagcaggac
     1681 gcgatcggtt cggtcctcga ggcgatcgag gaacaggtgg tgttgctccg actgctccac
     1741 gagatggcgc gcgactcgac ggacgtcgcg gtgcgcatcg gcggcgagac gcatcacgag
     1801 gccctgtcga agacctcgat cgtcgcggcg ggctacgggc cccccggcgg cgaaagttca
     1861 ctcctcggcg ccctcggccc gacccgcatg gactatcccg gcaccatggc cgcggtgcgc
     1921 gccgtagcgc gctacctctc acgagtggtg gaaatgtgac ggattactac gaggtcctcg
     1981 gcgttccccg aggcgccacc caggaccaga tcaagagggc ctaccgggcc ctcgcgcgcg
     2041 agcatcatcc cgacacgggc gacggcagcg acgaggagcg gttcaaggcc gtgtcgcggg
     2101 cgtacgaggt gctctcgacg ccggagaagc gcgcccggta cgacgccggg gtggacccga
     2161 gcggccgcgg cggcgcagcc gggttcggct tcgaggacat cctccaaacg ttcttcggcg
     2221 gtgggggtgg cgcgcgccgc ggcccggcgt cgcggaccca gcgcgggggc gacaccctga
     2281 tccacgcgga ggtctcgctc gaggaggcga ccttcggcgt cgcgcgcgac gtgactttga
     2341 gcctcgccga cccgtgcgcg acgtgcttgg ggacgtgttg tgccccgggg acctcgccca
     2401 agacgtgccc ctcctgcggc ggcacgggcg tcgtccaacg cgtcgcccga tccctgctcg
     2461 gccaggtgat gtccaccggc ccgtgtcccg cgtgcggcgg ctacgggacg acgatcgagg
     2521 ccccgtgcac cgagtgttcc ggccaggggc gcaccaactc caggcacacg atcaccgtcg
     2581 acattccc
//
LOCUS       DASVKP010000063         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_841241, whole genome shotgun sequence.
ACCESSION   DASVKP010000063 DASVKP010000000
VERSION     DASVKP010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841241"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VF362_01290"
     CDS             complement(<1..723)
                     /locus_tag="VF362_01290"
                     /inference="COORDINATES: protein motif:HMM:NF025833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEX7589594.1"
                     /translation="MNARARKATIAVGVTLIVGVVLAVALKGCALMPWNGSAAGGRGG
                     SVSLSVTRDFGRRAVANKAVSVGRGDSVMSVLGRSVDVDTDYGGGFVSSIEGLASRTS
                     SGSGNDWFYYVNGVLSGTGAAEYEVRDGDSVWWDYHPWGGRTFIPAVVGSYPSPFTRG
                     YGGARTATTVFYGPTLETLARKVGAYLEGLGARVEYRSGLPTSPGAGVEGPSMVFATP
                     ADAGGNRGVGKPLGDPAANGVFL"
     gene            complement(758..1540)
                     /locus_tag="VF362_01295"
     CDS             complement(758..1540)
                     /locus_tag="VF362_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589595.1"
                     /translation="MAVNANGEVVAERVMYFKGIAPILGASPNGVHNSVGARKAGKEW
                     YFAEGTTRPNFREWLCLMNPGGQATVANVSYFTGDGEVVTRQKDLPANSRTTVDVGAD
                     LGKPTDVSVSVTSEKPLVAERPMYFEYAPSTGGGTQPWGGGHNSIGTSYGAYKWEFAE
                     GTTRPGYETYLCIANTNDRDVVVAVDYIINKDGASRKKEKKVTVKKNSRYTIQVKDEV
                     EPGCDVSFELTSGVPVIVERPMYFGAGPYAGGGTTLGYCEEF"
BASE COUNT          232 a          589 c          457 g          276 t
ORIGIN      
        1 gaggaagacc ccgtttgcgg ccgggtcccc gagcggcttg ccgacgcccc ggttccctcc
       61 cgcgtccgcg ggcgtcgcga aaaccatcga cggcccttcc acgccggcgc ccgggctcgt
      121 gggcaggccg gaccggtact ctacgcgtgc ccccagcccc tccaggtagg ctccgacctt
      181 cctggcgagc gtctccagcg tcggcccgta gaacacggtc gtcgcggtcc tcgcgccccc
      241 ataccccctc gtgaacgggc tgggatagga acccaccacg gcgggaatga acgtgcggcc
      301 cccccagggg tggtagtccc accagaccga atccccgtcg cgcacctcgt actccgccgc
      361 gcccgtcccg gagagtacgc cgttcacgta gtaaaaccag tcgttcccgc ttcccgagga
      421 tgtccgcgat gcgaggccct cgatggacga cacgaagcct ccgccgtagt cggtatccac
      481 gtccaccgac ctgccgagaa cgctcatcac cgaatcgccc cggcccacgc tcaccgcctt
      541 gttagccacg gctcgccgcc cgaaatcgcg cgtcacgctc aggctgacgg agccgccgcg
      601 gccgcccgcc gcgctcccgt tccagggcat cagggcgcag cctttcagcg cgacggccag
      661 gaccacgccc acgatgagcg tcaccccgac cgcgatggtc gccttcctgg cccttgcgtt
      721 catccgggac ctcgcgtccg cgcgcacccg cgccggctca gaactcctcg cagtaaccga
      781 gagtcgtccc gcctcccgcg tacggccccg ccccgaagta catcggccgc tctactatca
      841 cgggcacccc tgaggtcaac tcgaacgaga cgtcgcaccc cggctcgacc tcgtccttca
      901 cctgtatggt gtagcgggag ttcttcttga ccgtgacctt cttctctttc ttcctggagg
      961 ctccatcctt gttgatgatg tagtcgaccg ccaccacgac gtcccggtcg ttggtgttcg
     1021 cgatgcagag gtatgtctcg tagcccggcc tcgtcgtgcc ctcggcgaac tcccacttgt
     1081 acgcgccata cgaagtcccg atagagttgt ggccgcctcc ccatggttga gtcccgccac
     1141 cggtcgaagg cgcgtactcg aagtacatcg ggcgctcggc gacaaggggc ttctcggagg
     1201 tgaccgatac gctgacgtcc gtcggcttgc ccaggtcggc gccgacgtcg accgtggtcc
     1261 tcgagttggc ggggaggtct ttctgccggg tgacaacttc gccatccccc gtgaagtagc
     1321 tgacgtttgc gacggtcgcc tggccgcccg gattcatcag gcacagccac tcacggaagt
     1381 tcggccgcgt ggtcccctcg gcgaagtacc attctttgcc ggccttgcgc gccccgacag
     1441 agttgtggac gccattgggg cttgcgccga gtatcggcgc tatccccttg aagtacatga
     1501 cgcgctcggc gacgacttcg ccgttcgcgt tgaccgccac cgacacgtcc cttc
//
LOCUS       DASVKP010000064         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_923101, whole genome shotgun sequence.
ACCESSION   DASVKP010000064 DASVKP010000000
VERSION     DASVKP010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923101"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VF362_01300"
     CDS             complement(<1..1212)
                     /locus_tag="VF362_01300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX7589596.1"
                     /translation="MSRDDARSGATGRVGAGVATGKARTVEAIPASALVARARALLSD
                     GFRLGLIAAHDEGSRLRIVYLFLAGSPDRRVELHVSVPALSPSVPSLGSLSFSASRFE
                     RAMHDLFGVVPTDHPLPHRLVRHAHWPSGWYPMRRDAGPTPPFTRHDPFPFVEVEGDG
                     VYEVPVGPVHAGLIEPGHFRFSVVGESVIKLKARLWFTHRGVEKLFEGMNALGATPLA
                     ERISGDTSAAHALAHVLAVEDALGLKVPSPARQLRAMLVEMERLYNHAADIGALAMDV
                     GFSLANSHAQRIREELLRINEACTGSRLLRGAIYPGGVRLLAAPDPVRIRLLAADLAE
                     VAALTLANTVIRDRFAGTAVLSERQVRDLGCLGYVARASGVDVDARIDHPSADLADLA
                     SSADLASSADHA"
     gene            complement(1209..2678)
                     /locus_tag="VF362_01305"
     CDS             complement(1209..2678)
                     /locus_tag="VF362_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX7589597.1"
                     /translation="MVSLLFLPIAAPLFAAGLAAIFGWRRWTAVVTAVSALTILATGV
                     ALAFFTDGEELVDSHHLLRADALSSTMLLVIGAVATLSCLASIGYIDAELADGHTTAQ
                     GARLYGVLLPLFLAAMALAVMAMSIGVMWVGIEATTVVTAFLVGHRRTKAALEATWKY
                     VIICSFGIALAFLGTVLTYFAGTTTGEPGNHSLDIDVLLSGAARLDPRVTSLAAGLLL
                     LGYGAKAGLVPFHTWLADAHSQAPAPVSALMSGVLLSVAFYALLRVKSIIDLTLGEGF
                     MRVGLIVMGLLTLVVAALLLVVQTDFKRMLAYSSMENMGIVAIAAAIGTRLAIAALLL
                     HVLAHGVAKALVFVSSGAIQGAHGSTAIAKVRAVLARSPLVGSAFTLGLVVLLGLPPF
                     AMFASEWGVARALAAEHFGWLLGVMLVFVAIAFTGLVRAGSSMMLGAPQAGEAHFAPP
                     RAIGATLALGVGLSVALCFSIGPLTNLLDAAATTLGAGR"
     gene            complement(2678..>2909)
                     /locus_tag="VF362_01310"
     CDS             complement(2678..>2909)
                     /locus_tag="VF362_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589598.1"
                     /translation="VLVSRRRAVSQAIGFLMLDNGIAATAFLITAGVPLIVELGASLD
                     ILFAMIVLGVLTDRMRQSFGDTDLNQLRELRD"
BASE COUNT          505 a          944 c         1108 g          352 t
ORIGIN      
        1 ggcatggtcg gcggagctgg caaggtcggc ggagctggca aggtcggcga ggtcggcgga
       61 ggggtggtcg atgcgggcat cgacgtcgac cccgctcgcg cgtgcgacgt agcccaggca
      121 accaaggtcg cgcacctggc gctcggacag gacggccgtg cccgcgaagc gatcgcgaat
      181 gacggtgttg gcaagcgtca gggcggcgac ctcggcgagg tcggccgcga ggaggcgaat
      241 gcgcacgggg tcgggtgccg cgaggagccg cacgccgccg gggtagatgg cgccgcgcag
      301 gagtcggctg cccgtgcacg cctcgttgat gcgcaggagc tcttcccgaa tccgctgcgc
      361 gtgggagttc gcgagcgaga agcccacatc catcgcaagc gctccgatgt ccgcggcatg
      421 gttgtagaga cgctccatct ccacgagcat ggcgcgcagc tggcgggcag ggctgggcac
      481 cttgaggccg agggcgtcct cgacggcgag gacgtgcgcc agggcgtggg ccgccgacgt
      541 gtccccgctg atgcgctcgg cgagcggcgt cgccccgagc gcgttcatgc cctcgaagag
      601 tttctcgacg ccgcggtggg tgaaccacaa ccgcgccttg agcttgatga cgctctcccc
      661 tacgacggag aagcggaagt gccccggctc gatcaggccc gcgtggacgg ggccgaccgg
      721 cacctcgtac accccgtccc cctcgacctc gacgaacggg aagggatcgt ggcgggtgaa
      781 gggaggcgtc gggcccgcgt cgcgccgcat cgggtaccac cccgacggcc agtgggcgtg
      841 tcggacgagg cgatgcggga gggggtggtc ggtggggacg acgccgaaga ggtcgtgcat
      901 cgcgcgctcg aagcgcgacg cggagaacga gagcgatcca agggacggca cgctggggga
      961 gagcgcgggg accgagacgt ggagttcgac gcggcggtcg ggcgagccgg cgaggaagag
     1021 gtacacgatt cgaagcctcg agccctcgtc atgggcggcg atgaggccaa ggcggaatcc
     1081 gtcggaaagc agggcccgcg cgcgagcgac gagggccgag gccgggatcg cctcgacggt
     1141 tctcgccttg ccagttgcga cgcccgcccc tactcggccc gttgccccgc tccgagcgtc
     1201 gtcgcggctc atcgccccgc tccgagcgtc gtcgcggcgg cgtcgagcag gttcgtcagc
     1261 ggcccgatgc tgaagcacag cgcgacgctg aggccgacgc cgagggcgag ggtcgcgccg
     1321 atcgcgcgcg gaggagcgaa gtgcgcctcg cccgcctggg gcgccccgag catcatggag
     1381 gacccggcgc gcacgagccc ggtgaacgcg atcgcgacga acacgagcat cacgccgaga
     1441 agccagccga agtgctcggc ggcgagcgcg cgcgccacgc cccactcgct ggcgaacatc
     1501 gcgaagggcg gaaggccgag caggacgacg agtccgagcg tgaacgccga ccccaccagc
     1561 ggcgaccgcg cgagcaccgc gcgcaccttc gcgatggccg tcgatccgtg ggccccctgg
     1621 atggcaccgg aggaaacgaa gacgagggcc ttggcgacgc cgtgagcgag gacgtgaagc
     1681 aggagcgccg cgatggccag gcgcgtgccg atcgccgcgg cgatggcgac gatccccatg
     1741 ttttccatcg aggagtacgc gagcattcgc ttgaagtccg tctgcacgac taggagcaac
     1801 gccgcgacca cgagcgtcag gaggcccatc acgatgagtc ccacgcgcat gaacccttcg
     1861 ccgagggtca ggtcaatgat cgacttgacc cggaggagcg cgtagaacgc cacggacagt
     1921 agcacgccgc tcatgagggc ggagacgggc gcgggggcct gactgtgcgc gtcggccagc
     1981 caggtgtgga acgggaccag gcccgccttc gccccgtagc cgaggagcag gagccccgcc
     2041 gcgaggctcg tcaccctggg gtcgagccgt gccgccccgg agagcagcac gtcgatgtcc
     2101 agggagtgat tgccgggctc gcccgtcgtc gtccctgcga agtacgtcag gaccgtcccg
     2161 aggaaggcga gggcgatgcc gaacgagcag atgatgacat acttccacgt cgcctcgagg
     2221 gccgccttgg tccgccggtg gccgacgaga aacgcggtga cgaccgtcgt cgcctcgatg
     2281 ccgacccaca tgaccccgat gctcatcgcc atcacagcga gggccatggc tgcgaggaac
     2341 agcgggagga ggacgccata gaggcgcgcg ccctgggccg tcgtgtggcc gtccgccagc
     2401 tccgcgtcga tgtagccgat gctcgcgagg cacgacagcg tggccacggc gccgatgacg
     2461 aggagcatcg tggaactcag ggcgtcggcg cgcaggaggt ggtgggagtc gacgagctcc
     2521 tcgccgtcgg tgaagaaggc gagagcaacg cccgtcgcga ggatcgtgag cgcggacacg
     2581 gcggtgacga cggcggtcca gcgccgccag ccgaagatcg ccgcgagccc ggccgcgaag
     2641 agcggtgccg caatggggag gaagaggagg ctgaccacta gtctcgcaac tccctcaact
     2701 ggttgagatc ggtgtcgccg aacgattggc gcatgcgatc ggtcaggacc ccgaggacga
     2761 tcatcgcgaa gaggatgtcc agcgatgccc cgagctcgac gatgaggggc actcccgccg
     2821 tgatgaggaa ggcggtcgcg gcgatcccgt tgtcgagcat gaggaatccg atcgcctggg
     2881 acacggcgcg cctgcggctc accaggacg
//
LOCUS       DASVKP010000065         9811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049 k141_17779,
            whole genome shotgun sequence.
ACCESSION   DASVKP010000065 DASVKP010000000
VERSION     DASVKP010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17779"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VF362_01315"
     CDS             <1..646
                     /locus_tag="VF362_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX7589599.1"
                     /translation="GAGNEWLDQDPWRVLRIQSEFVEGFGALSHLGRAVCVFGSARSK
                     AGTPEYAMAETMGRLLAEAGFAAITGGGPGTMEAVNKGASLAGGVSVGLGIELPHEQA
                     LNDYVNLGINFRYFFVRKTMFLKYAQGFIVLPGGFGTLDELFESMTLVQTDTVRDFPI
                     VLVGSAYWAPLLAWLRDTVAKDGKIAADDLDLVTITDDPVEAVVLATSYHSQTG"
     gene            742..909
                     /locus_tag="VF362_01320"
     CDS             742..909
                     /locus_tag="VF362_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HEX7589600.1"
                     /translation="MAAMKPRTGDGPMEVVKEGRGFILRLPLEGGGRLVVEMSADEVR
                     QLGGTLQAAVS"
     gene            complement(952..1584)
                     /locus_tag="VF362_01325"
     CDS             complement(952..1584)
                     /locus_tag="VF362_01325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX7589601.1"
                     /translation="MTHEALANWAYCEDFVPEDEVLRRARDVAEALGCVPVMPGAGAV
                     LTAMALAIQARTVAEIGTGTGVSAVYLLRGMHADGILTSIDVEPESQKAARQTIAAAG
                     FPPERVRMISGAALDVLPRLTDGGYDFVLIDAIKSEYPAYLEHALRIVRTGGVIAIDN
                     ALWHSKVADPAQRDHNTTAVRETLKAVRASEELTPVLLPSGDGLLIAIRR"
     gene            1708..2286
                     /gene="sigE"
                     /locus_tag="VF362_01330"
     CDS             1708..2286
                     /gene="sigE"
                     /locus_tag="VF362_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigE"
                     /protein_id="HEX7589602.1"
                     /translation="MLVETEATFAPTAPDLTWEGIVRDHSARVYRLALHLTGNPHDAE
                     DLTQDVFVRVFNSLSQYKPGTFEGWLHRITTNLFLDRVRRRKRIRFDWMGDDDGAVAT
                     ADSFDRHERSGQPEDAFEMVHLGPDILDALAELPPEYRAAVVLSDIEGLSYEEIAATL
                     GIKMGTVRSRLSRARARLRETLAHRAPEDGGA"
     gene            2283..3128
                     /locus_tag="VF362_01335"
     CDS             2283..3128
                     /locus_tag="VF362_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589603.1"
                     /translation="MTTRHLGKRLYALVDGRLSREDAYVAMAHMGECTECAAEWEALR
                     KDRIALQTSGTGIDMSFTNHLLDRDRIAEIARDEPRRHVRAASGRVMNVAVVGVSLFA
                     GGALFLGILWKLGAPPEVGLEVALAPDEATASSVQLMGAPRMRLEGSLVSWVHPAWET
                     STLIPVDASVIHTADGTTVLVARVLAGNDSITVIERKGTLPAGFSSEFPMGGVAGREV
                     YVVSDHPTQVVWDAGGVVVGLGCTCSLDVLQSVASQFPNGNEPGVLERLGDGLRAVAD
                     AVTGG"
     gene            3131..4426
                     /locus_tag="VF362_01340"
     CDS             3131..4426
                     /locus_tag="VF362_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7589604.1"
                     /translation="MKEEEGLFADPSIPASEAPRAFAAEAPRSKPARTLDEPVIVPTL
                     ETDSAGRGPAPARTGLGPMWVMVVSVLALVFGAAGGVGGYAIASHGDSAVTDSLPHAG
                     VNVDAPVAQPGSVTDVVAAVLPSVVRIEASSGGTLVDTGSGFVIRADGYILTNDHVVA
                     PSDGELNVRFSDGTVSPATLVGTTSDYDLAVLKVDKTGLTPLVLADSDKVRVGDEAIA
                     IGSPLGLDSTVTLGIVSSLHRPVTAGDSASSTAFIDAIQTDAAINPGNSGGPLLNASG
                     EVIGINSATAALPGASQQSGSGTVGLGFAIPSNQARRTADEIIATGKATFPVIGVLLD
                     STYHGEGVRVINDDPATKTFGVAPGSPAALAGIKAGDIILSFNGRPVTMPEDLVVATR
                     AMAPGDVITLEVKSGSSTSTVRVTLTANDKITWNDGSQQ"
     gene            4436..4783
                     /locus_tag="VF362_01345"
     CDS             4436..4783
                     /locus_tag="VF362_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase TatB"
                     /protein_id="HEX7589605.1"
                     /translation="MLDINGQEFVILLLVATVVIGPERLPRYAREFRGLAAKARDLLG
                     DVRASVRGEMDDTVELAALNPRQSDSHRVVKDVAADGDSSSPSARSDGRAARRRSARI
                     ATGAVPPFDADAT"
     gene            4835..5836
                     /gene="trpS"
                     /locus_tag="VF362_01350"
     CDS             4835..5836
                     /gene="trpS"
                     /locus_tag="VF362_01350"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX7589606.1"
                     /translation="MSPRVFSAMQPTADSLHLGNYLGALVQWVALQETHDTIYAVVDL
                     HALNEHPEPEALTRRTRATAAQFLAAGVDPARSLLFVQSHVPQHAELAWVLGGFTGFG
                     EASRMTQFKDKSAKQGERGTNVALFTYPVLMAADILLYDAAAVPVGEDQRQHLELTRD
                     LAQRVNASYGDIFTVPEPHIIKATAKIYDLQDPSSKMSKSGGPGGIIEILDEPKVIAK
                     RIRSAVTDAETEVRFDERLKPGLANLLTIYSALSGRSISDIEDDFAGKGYGDFKGALA
                     DVVIEFLAPIREAALGWIESPDKLDGVLADGAEKARVIAQGTLERVYDAVGILRPAR"
     gene            5843..6751
                     /locus_tag="VF362_01355"
     CDS             5843..6751
                     /locus_tag="VF362_01355"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX7589607.1"
                     /translation="MTGANGGFDAGGGPRLRRLVTGASGILETFKATRAGLAVERYSR
                     GRGNVLAGGVAYNALLSVGAAVVVASTVAALVVGRAPSLRDAMLRFLDRAIPGIVDTG
                     AGNGLIKASSLAVSPMTGLVSALAVVIGLNTVTRYAGALRAATQAMLGSESRSPLHGK
                     SRDVLALGVLGLMALLAAVLQVVAASAAAWLGADQSGRTWAVRAVAFVLVLAADALFV
                     FVALAVLGGAGRPHRRLFPAIAAVAIAIAILRSLSTLVLGASIGNPILAPFAAVIMVL
                     VWVDLVTRLVLLAAAWVGAGHPLRTT"
     gene            6765..8300
                     /locus_tag="VF362_01360"
     CDS             6765..8300
                     /locus_tag="VF362_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589608.1"
                     /translation="MDAVRSRRQWGPRLWVVAMVAGTSLVGCTFSKGDPYGDLPPAPT
                     AETVLEDINLAAFGLRSYPCDLFEASVSRCYRPDHWLSIREGRETLAHVHRELVGAGA
                     RFWGGGESLVKADRPSVAGLNLGCYFDYATRDLDLRVAIVGPTGEAAPCVNGLPGDGV
                     REVWIVGGNFDPGDVYSSVSMPPSFESLLIAVPGPPPVLGGVYGGTSSTVDGYYGMPL
                     PARPQGAGVKLLPETISVRDGIVRGLVQYRGAAAINVPAPSAVPAPSAAPVLTAAPAP
                     SGVTSASVPAEASPVAEEGGAVGVVIGLGTSRYAVPIIIRPGESAPFELPLPDGFTPG
                     DLKVIPGWISVDDTWHGAQLVSGPLIDPACAEALTVEGDAIAPLAPGPGQSCYVAFAQ
                     ATMAPGTAIFADAMVAIFDTDGTVVDVIQPYLIRGDGSLAGQGRLMTYDNDLRLGWVD
                     VSGREAATIGIWVRYARGSDMVLGGSGDPAAAAEAAIAAAEAAAAAAAPTAAAPTATI
                     APG"
     gene            complement(8313..9110)
                     /locus_tag="VF362_01365"
     CDS             complement(8313..9110)
                     /locus_tag="VF362_01365"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX7589609.1"
                     /translation="MRATATKATTTKATATKATATGEVAMFEVILKIRRNNPEASGEH
                     EAGTGYWQEFTVRVHSTERILDALHTIKWEQDGSLTFRRSCAHGVCGSDAMRINGRNR
                     LACKALIKDLNPSRPITIEPIQGLPVLKDLIVDMEPFFASYREIMPFLIPSGDAPKRE
                     RLQSPEQRARFEDTTKCILCAACTTSCPVFWTDGQYFGPAAIVNAHRFIFDSRDDAAR
                     ERLEILNDKDGVWKCRTTFNCTDACPRGIEVTEAISQVKRALTTGRT"
     gene            complement(9110..>9811)
                     /locus_tag="VF362_01370"
     CDS             complement(9110..>9811)
                     /locus_tag="VF362_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7589610.1"
                     /translation="PTAHYALGGIPTPVTGEVLRNATETVDGLYSAGECACVSVHGAN
                     RLGTNSLLDINVFGRRAGAAAAMHAAKAPQPSMPVDAAARVVEIVERLLAGTGDASSP
                     RPGDIRAELQEAMDRDAQVFRTAESLTHVQAKVPGLKERFARATIQDKGRRFNTDLLE
                     AIELGFLLDLAEVILAGALAREESRGGHFREDFPTRDDKKFLKHTLAYATPTGITLDY
                     KPVVITKYEPKERVY"
BASE COUNT         1459 a         3305 c         3396 g         1651 t
ORIGIN      
        1 gggcgcgggc aacgagtggc tcgaccagga cccctggcgg gttcttcgaa tccagagcga
       61 gttcgttgag ggattcgggg cgctttctca ccttggccgc gcggtatgcg tctttggatc
      121 ggcgcgctcg aaggcaggga cgcccgagta cgcgatggca gagacgatgg ggaggctcct
      181 cgccgaggcc ggattcgccg cgatcaccgg cggaggcccc ggaacgatgg aggccgtcaa
      241 caagggtgcc tccctggcgg gcggcgtctc cgtcggactt ggcatcgagt tgccccacga
      301 acaggcgctc aacgactacg tcaacctggg catcaacttc cgctacttct tcgtgcgcaa
      361 gacgatgttt ctcaagtacg cacaggggtt catcgtgctt ccgggaggct tcggcacgct
      421 cgatgagctc ttcgaatcca tgaccctggt ccagacggac accgtccgtg acttcccgat
      481 tgtcctggtc ggcagcgcct actgggctcc gctcctcgcc tggctgcggg acacggtcgc
      541 gaaggacgga aagatcgccg ccgacgacct cgacctcgtc acgatcacgg acgatccggt
      601 agaggccgtc gtgctcgcga cgtcctacca ctcgcagacg gggtaggcga aggggaccgc
      661 atttctgcgc gcccgaatgt cccgctagaa tgggatgcgc cgcgcgctgc ggtgttgcga
      721 attctaggag aagagcaccc catggctgct atgaagcccc gcaccggcga cggccccatg
      781 gaggtcgtca aggagggacg cggctttatc ctgcgcttgc cgctcgaggg aggcggtcgt
      841 ctcgtggttg agatgagcgc cgacgaggtc aggcaactcg gcggcaccct ccaggccgca
      901 gtctcgtagg ccgcagcctc gtaggtcgca gtctcgtagg tcgcggcgga gtcatcgccg
      961 aatcgcgata agcaggccgt ccccggacgg caggagcacg ggcgtgagtt cttcgctcgc
     1021 gcgcacggcc ttgagcgtct ctcgcaccgc cgtggtgttg tggtcgcgtt gcgcgggatc
     1081 cgcgaccttc gagtgccaga gggcgttgtc gatggcgatg acgccccccg tgcgaacgat
     1141 cctcagcgca tgctcgaggt aggccgggta ctcggacttg atcgcgtcga tgagcacgaa
     1201 gtcgtatccg ccgtccgtca gtctgggtag gacgtcgagc gccgctccgg agatcatgcg
     1261 aacccgctcc ggcgggaacc ctgccgcggc gatggtctgc cgcgccgcct tctgactctc
     1321 cggctcgacg tcaatgctcg tcaggatgcc gtcggcatgc atcccccgaa gtaggtagac
     1381 ggcagacacc ccagtccccg tcccgatctc ggcgaccgtc ctcgcctgga tggcgagcgc
     1441 catcgcagtg aggaccgcac ccgcacccgg catcacgggc acgcacccga gggcctcggc
     1501 gacgtcgcgg gcgcgccgaa gaacctcgtc ctcgggaacg aagtcctcgc agtacgccca
     1561 gttggctaga gcttcgtgtg tcatggccct cctggagttc atcgtatcgg cgccaccgtg
     1621 ggaacatggt ggggactcaa gtcgttaccc acctatggag gtgttgcggt gacccacccg
     1681 cccgtcccgc cgcgccccgg cgcggcgatt ctcgtcgaga ctgaagcgac tttcgcgccc
     1741 accgcacccg acctcacgtg ggagggcata gtccgcgacc actcggcgcg cgtgtaccgc
     1801 ctcgcgcttc acctcacggg caacccgcac gacgccgagg acctcacgca ggatgtcttc
     1861 gtccgcgttt tcaattcgct ctcgcagtac aagcccggga cgttcgaggg atggcttcat
     1921 cgcatcacga cgaacctctt cctcgaccgc gtgcgccgac gcaagcgcat tcggttcgac
     1981 tggatgggtg acgacgacgg cgcggtcgcg acagccgata gtttcgaccg gcatgagcgt
     2041 agcggccagc ccgaggacgc cttcgagatg gttcatctgg ggcccgacat cctcgacgcg
     2101 cttgcggagt tgccgcccga gtaccgcgcg gcggtggtcc tgagcgacat tgaagggctc
     2161 tcctacgagg agatcgcggc caccctggga atcaagatgg ggacggtgcg ctcgcgcctg
     2221 tcccgcgcgc gggcacgcct gcgggagacc ctcgcccaca gggcgccgga ggatgggggg
     2281 gcgtgaccac gcgccacctc ggcaaacggc tctacgctct ggtcgacgga cgcttgagcc
     2341 gcgaggacgc ctacgtcgcg atggcccata tgggcgaatg cacggagtgc gccgccgagt
     2401 gggaagccct ccggaaggac cggatcgccc tgcagacctc gggcaccggc atcgacatgt
     2461 cattcacgaa ccacctgctc gatcgcgatc gcatcgccga gattgcgcgc gacgagccga
     2521 ggcggcacgt gcgggcggcc agcggcaggg tcatgaacgt ggcggtggtg ggcgtgtctc
     2581 tcttcgccgg tggcgcgctc ttcctgggga tcctgtggaa gctcggcgcc cctcccgagg
     2641 tcgggcttga ggtcgcgcta gcccccgacg aggcgacggc gtcgagcgtc caactcatgg
     2701 gcgcgccgcg catgcggctc gagggctctt tggtgtcttg ggttcacccg gcgtgggaga
     2761 cgtcgacgct gatccccgtc gacgcctcgg tcattcacac ggcggacgga acgacggtgc
     2821 tcgtcgctcg cgtcctggca ggcaacgact cgatcaccgt gatcgagcgg aaaggaacac
     2881 ttcccgcggg tttcagcagc gaattcccga tgggtggcgt agcgggccgc gaggtctatg
     2941 tcgtatcgga tcatcccacg caggttgtgt gggatgccgg aggagtggtg gtcggtctcg
     3001 gctgcacctg ctcgctcgac gtactccagt ccgttgcgag tcaattcccc aacggaaacg
     3061 aaccgggcgt attggaacgc ctgggggatg gccttcgggc cgtcgctgat gcagtaacgg
     3121 gaggttaacc atgaaggaag aagaagggct gttcgctgac ccgagcatcc cggcgagcga
     3181 ggcaccccgc gctttcgcgg cggaggcacc gcggtcgaag ccggcccgca cgctcgacga
     3241 gcccgtgatc gtccccacgc tcgagaccga cagcgccggt cgcggcccgg cgcccgcacg
     3301 aacgggactt ggcccgatgt gggtgatggt cgtgtctgtc ctcgcgctcg tgtttggcgc
     3361 tgcgggaggg gtgggtggct atgccatcgc ctcgcacggc gattcggcgg tgacggacag
     3421 ccttccgcac gccggcgtga acgtggacgc gcccgtggcg cagcccggtt ccgtcaccga
     3481 tgtcgtcgcg gcggtgcttc cgtccgtcgt gcggatcgag gcgtcgtccg gagggacgct
     3541 cgttgacacg gggtccggct tcgtcattcg ggcggatggc tacatcctca ccaacgacca
     3601 cgtcgtcgcg cccagcgacg gagaactcaa cgttcgcttc tccgacggca cggtctcgcc
     3661 ggcaaccctt gtgggcacga cgagcgacta cgacctcgcg gtgctcaagg tcgacaagac
     3721 ggggctcacg ccgctcgtgc ttgctgactc cgacaaggtt cgggtcggcg acgaggcgat
     3781 cgccatcggt tccccgctcg gcctcgactc caccgtgacg ctcgggatcg tgagctcgct
     3841 ccaccgcccc gtgaccgcgg gcgacagcgc aagttcgacc gcgttcatcg acgccatcca
     3901 gacggacgcg gcgatcaacc ccgggaactc cgggggtccg ttgctcaacg cctccggcga
     3961 ggtcatcggg atcaactccg ccaccgcggc gcttcccggt gcgagccagc agtccgggtc
     4021 cggcaccgtc ggcctcggct tcgcgatccc ctcgaaccag gctcggcgca cggccgacga
     4081 gatcatcgcg acagggaagg ccaccttccc cgtgatcggc gtgctgctcg acagcacgta
     4141 ccacggcgag ggagtcagag tgatcaacga cgacccggcg accaagacgt tcggcgtcgc
     4201 tcccggatcg ccggcggccc tggcgggaat caaggcgggc gacatcatcc tgtcgttcaa
     4261 cgggcgcccg gtcacgatgc ccgaggacct cgtcgtcgcg acgcgggcga tggccccggg
     4321 cgacgtcatc accctggagg tcaagtccgg aagttcgacc tccacggtca gggtcaccct
     4381 cacggcgaac gacaagatta cgtggaacga cggatcgcaa cagtaggctg acacggtgct
     4441 cgacatcaac ggccaggagt tcgtcatcct gctcctggtc gccactgtcg tcatcggccc
     4501 cgagaggctc ccgcgctatg cgcgggagtt tcgggggctg gcggccaagg cgcgggacct
     4561 cctcggcgat gtgcgcgctt ctgtgcgggg cgagatggac gacacggtcg aactcgctgc
     4621 cctcaacccc cggcagtccg actcccatcg cgtcgtcaag gatgtggccg cggacgggga
     4681 ttcgtcgtcg ccgtcggccc ggtccgacgg cagggcggcg cgacgacgct ccgcgcggat
     4741 cgcgaccggt gcggtgcccc cgttcgacgc ggacgccacg tagccactcg gcgctgcgcc
     4801 gtcgaaggcg ccgcgacctg gaacaatggg cggcatgtct ccacgcgtct tctccgctat
     4861 gcagcccacc gcagactccc ttcacttggg gaactacctg ggggccttgg tgcagtgggt
     4921 ggcgctccag gagacccacg acacgatcta cgccgtggtc gacctccatg cgctcaacga
     4981 gcaccccgag ccagaggcgc tgacaaggag gacgcgcgcg accgccgccc agttccttgc
     5041 ggccggggta gaccccgcgc gctcgctcct ctttgtgcag tcccacgtgc cgcaacatgc
     5101 cgaactcgca tgggtgctcg ggggcttcac ggggttcggc gaggcgagcc ggatgaccca
     5161 gttcaaggac aagtccgcca agcaggggga gcggggcacg aacgtcgccc tcttcacgta
     5221 cccggttctc atggccgccg acatcctcct ctacgacgcc gcggcggtgc ctgtcggcga
     5281 ggatcagcga cagcacctcg agttgacccg cgacctcgcg cagcgggtca acgcgagtta
     5341 cggcgacatc ttcaccgtcc ccgagcccca catcatcaag gcgaccgcga agatctacga
     5401 ccttcaggat ccgtcctcga agatgtcaaa gtccggcggc cccggcggaa tcatcgagat
     5461 cctggacgag ccgaaggtca tcgcgaagcg catccgctcc gcagtcacgg acgccgagac
     5521 cgaggtccgt ttcgacgagc ggctcaagcc gggtctcgcg aacctcctca ccatctactc
     5581 cgcgctctcg ggacgttcca tctcggacat cgaagacgac ttcgcgggta aggggtatgg
     5641 ggacttcaaa ggggcacttg ccgacgtggt catcgagttc cttgcgccca tccgcgaggc
     5701 cgcgctcgga tggattgagt ctccggacaa gctcgacggc gtcctcgcgg acggcgcgga
     5761 gaaggcacgg gtgatcgcgc agggcacgct cgagcgcgtt tacgacgccg tcggaattct
     5821 ccggccggcg cgctaggcga cggtgaccgg cgcgaatggc gggttcgacg ccgggggggg
     5881 gccgcgcctc cgtcgcctcg tcacgggggc gagtggcatt ctagagacgt tcaaggcgac
     5941 gagggccggc ctcgcggtgg agcgctactc gcgcgggcgc ggcaacgtcc tcgctggcgg
     6001 tgtcgcctac aacgccctct tgtctgtcgg agcggcggtc gtggtcgcgt cgacggtggc
     6061 cgcgctggtg gtgggtcggg cgccgtcgct tcgcgacgcc atgctccgat tcctcgaccg
     6121 tgccattccc ggcatcgtcg acacgggcgc gggcaacggc ttgatcaagg cctcgtcgct
     6181 cgcggtgagc cccatgacgg ggttggtgag cgccctcgcc gtggtcatcg gcctcaacac
     6241 ggtcacccgc tatgccggtg ccctgcgcgc cgcgacgcag gcgatgttgg gcagcgagtc
     6301 acgttcgccg ctccacggca agagccgcga cgtcctcgcg ctaggtgtgc tcgggctcat
     6361 ggcccttctg gcggcagtgc tccaggtcgt tgcggcgagc gcggcggcct ggcttggcgc
     6421 cgatcagtct ggccgtacct gggccgtgcg tgccgtggca ttcgtcctag tcctcgcggc
     6481 ggacgcactc ttcgttttcg tcgcgctcgc ggtgctcggc ggcgcggggc gcccccatcg
     6541 gcgcctgttt cccgccatcg cggccgtggc gatcgccatt gccatcctcc gctcgctctc
     6601 gaccctggta ctcggagcct ccattgggaa ccccatcctc gctcccttcg ccgccgtcat
     6661 catggtgctt gtttgggtcg acctcgtaac gcggctcgtg ttgctcgccg ccgcatgggt
     6721 cggcgcgggg catccacttc ggacgaccta ggtactctgg ggtggtggac gccgtgcgct
     6781 cgcgaaggca atgggggccg cgactgtggg tcgtcgcgat ggtggcgggt acgagcctgg
     6841 tcgggtgcac gttctccaag ggtgacccct acggtgacct gcccccagcg cccacggcgg
     6901 agaccgttct cgaggacatc aacctcgcgg catttggcct acgctcctat ccctgcgacc
     6961 tcttcgaggc ttccgtttcg cggtgctacc gacccgatca ctggctgtcg attcgtgagg
     7021 gcagagagac gctcgcacac gtgcatcgcg aactcgtggg ggcgggggcg cgattctggg
     7081 gagggggcga gtcgctcgtg aaggccgacc gtccgtccgt cgcggggctg aaccttggtt
     7141 gctacttcga ctacgcgacg cgcgaccttg acctgcgggt cgcgatcgtt ggcccgacgg
     7201 gggaggcggc gccgtgcgtc aacggacttc ccggcgacgg ggtcagggag gtgtggatcg
     7261 tcggggggaa cttcgacccg ggggacgttt actcgtccgt cagcatgccc ccatccttcg
     7321 aatcgctcct tatcgccgtg ccgggtccgc cgcccgttct ggggggggtc tacggcggga
     7381 cctcgtcgac cgtcgacggg tactacggaa tgccgttacc cgcgcggccc cagggcgccg
     7441 gggtcaagct cctgcccgag acgatctccg ttcgggacgg catcgtccga gggctcgttc
     7501 agtaccgcgg cgccgccgcg atcaacgtgc ccgccccgag cgccgtgccc gcgccgagcg
     7561 cagcgcccgt gctgaccgcc gcgcccgcgc cgagcggggt gacgagtgct tccgtgcccg
     7621 ccgaagcctc gccggtcgcc gaagaggggg gagccgtcgg cgtcgtcatc ggcctgggga
     7681 cttcacgcta tgccgtgccc atcatcatcc gaccggggga gtccgcgccg ttcgagcttc
     7741 cgctccccga cgggttcacg cccggggacc tcaaggtgat tcctgggtgg atcagcgttg
     7801 acgacacgtg gcacggggcg caactcgtga gcgggccact tatcgatccc gcgtgcgccg
     7861 aggcgctgac ggtcgagggc gacgcgatcg ccccgctcgc gccgggtccc gggcagtcgt
     7921 gctacgtcgc gttcgcccag gcgacgatgg cgcccggcac cgcgatcttc gccgacgcga
     7981 tggtcgcgat cttcgatacc gatggcacgg tcgtcgacgt catccagccg tacctcattc
     8041 gcggcgatgg gagcctcgcc ggccagggtc ggctcatgac gtacgacaac gacctccgac
     8101 tcgggtgggt ggacgtgtcg ggtagggagg ccgcaaccat cggcatctgg gtccgctacg
     8161 cgcgcggatc ggacatggtg ctcgggggct cgggcgatcc tgcggcggca gcagaggcgg
     8221 ccattgcggc cgcggaggca gccgcagcgg cagcagcccc aacggcagca gccccaacgg
     8281 caaccattgc cccgggctag atccgcccgg tgctaggtcc gacccgtggt gagcgcgcgc
     8341 ttcacctggg agatcgcctc ggtgacctca atgccgcgcg ggcaggcgtc ggtgcagttg
     8401 aaggtcgtgc gacacttcca cacgccatcc ttgtcgttga ggatctccaa gcgctcccgg
     8461 gcggcatcgt cgcgcgagtc gaaaatgaag cggtgggcgt tgacgatcgc tgcggggccg
     8521 aagtactggc cgtccgtcca gaacaccggg cacgacgtgg tgcacgcggc gcacaagatg
     8581 catttggtcg tgtcctcgaa gcgcgcccgc tgctcgggcg actgaaggcg ctctcgcttc
     8641 ggggcgtcgc cggaggggat gaggaacggc atgatctcgc ggtaggaggc aaagaacggc
     8701 tccatgtcca cgatcaggtc cttgaggacc ggaaggccct ggattggctc gatcgtgatc
     8761 gggcgcgacg gattgaggtc cttgatgagc gccttgcatg cgagccggtt ccgaccgttg
     8821 atgcgcatgg cgtccgaccc gcagaccccg tgcgcgcacg aacgccggaa cgtcagcgat
     8881 ccatcctgct cccacttgat cgtgtggaga gcgtcgagga tgcgctccgt cgagtggacc
     8941 ctaaccgtga actcctgcca gtagcccgtg ccggcctcgt gctccccgga cgcctccggg
     9001 ttgttccgac ggatcttgag gatgacctcg aacatcgcga cctctccggt cgcggtggcc
     9061 ttggtcgcgg tggccttggt cgtggttgcc ttcgtcgcgg tcgcgcgcat tagtagacac
     9121 gctccttggg ctcgtacttg gtgatgacga ccggcttgta gtcgagggtg atgcccgtcg
     9181 gtgtcgcgta ggcgagggtg tgcttgagga acttcttgtc gtcgcgcgtg gggaagtcct
     9241 cgcggaagtg gccgccccgc gactcctcgc gcgcgagggc gcccgcaagg atcacctcgg
     9301 cgaggtcgag caggaatccg agttcgatcg cctcgagcag gtccgtgttg aagcggcgcc
     9361 ccttgtcctg gatcgtcgcg cgcgcgaatc gctccttgag cccggggacc ttcgcctgca
     9421 cgtgggtcag gctttctgcg gtccggaaga cctgggcgtc gcggtccatc gcctcctgga
     9481 gttcggcgcg gatgtcgccg ggcctggggc tcgacgcgtc gcccgtcccc gcgaggagtc
     9541 gctcaacgat ctcgacgacc cgagccgcgg cgtcaaccgg catggatggc tggggggcct
     9601 tggcggcgtg catcgccgcc gcggctcccg cgcgcctgcc aaacacgttg atgtccagga
     9661 gcgagttggt gccgaggcgg ttcgcgccgt gcacggacac gcacgcgcac tcgcccgccg
     9721 agtacaggcc gtcgacggtc tcggtggcgt tgcgcagcac ctcgccggtg acgggggtgg
     9781 ggatgcctcc cagggcgtag tgagccgtcg g
//
LOCUS       DASVKP010000066        10198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_432168, whole genome shotgun sequence.
ACCESSION   DASVKP010000066 DASVKP010000000
VERSION     DASVKP010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10198
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432168"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VF362_01375"
     CDS             complement(<1..265)
                     /locus_tag="VF362_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589611.1"
                     /translation="MNASPGPIAGRLKRPRWSDPRLLIGVALIAIAILGTASVVARAD
                     QTEPYYAARHTLTPGTVLELSDVVVIHVAVGSGIYVPATTVPWG"
     gene            370..576
                     /locus_tag="VF362_01380"
     CDS             370..576
                     /locus_tag="VF362_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7589612.1"
                     /translation="MPPRFLTLADVEEVLNITATQAYALVRSGDLPAIQVGGRGQWRI
                     ESSELEAYIQRQYEATRARITTER"
     gene            complement(586..1113)
                     /locus_tag="VF362_01385"
     CDS             complement(586..1113)
                     /locus_tag="VF362_01385"
                     /inference="COORDINATES: protein motif:HMM:NF022298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2642 domain-containing protein"
                     /protein_id="HEX7589613.1"
                     /translation="MRWNDLFGDLEGQLSAAHASEFEAGVRDLAQAERATVRLAERLA
                     SRASAPVTLTLTLRGGSHVSGLVTEAAGSWVLLSEGGREYLVPRGAIAMARGVGHRAS
                     SFGAVTARLGLGHALRALAREGVPVVISTTGGEIRGRIDQVGADHLDLAMDDGVAVAV
                     SLAEVLVIASSGAAA"
     gene            1274..1699
                     /locus_tag="VF362_01390"
     CDS             1274..1699
                     /locus_tag="VF362_01390"
                     /inference="COORDINATES: protein motif:HMM:NF039460.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HEX7589614.1"
                     /translation="MSAQLAEMPVAGLVAVPFPGPRPPLNPYTRPRKVLGDPTPLACT
                     VAKVALEVVLGGAGLDQLTRWVTPDIREALAQQGALARRAGLRIGAPVRILRIRVFRV
                     SDVAAETSVVADDGVHARAIAARFEDVGGRWQATVLDVG"
     gene            complement(1779..4649)
                     /gene="secA"
                     /locus_tag="VF362_01395"
     CDS             complement(1779..4649)
                     /gene="secA"
                     /locus_tag="VF362_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX7589615.1"
                     /translation="MMRMGEGRELRRLARVARIAGGLESAYEAMSDGELRSLTEEFKQ
                     RRRDGESLDSLMPEAFAAVREASRRTLGLRHFDVQLMGGAVLHWGNIAEMKTGEGKTL
                     VATLPVYLNALDGLGVHIVTVNDYLARYQSELMGRVFRFLGLETGCILTGMEPQERRK
                     QYLADVTYGTNNEFGFDYLRDNMAMSMGELVQRGHVYAIVDEVDSILIDEARTPLIIS
                     GPAQGVNNRWYGEFAALVRTLKAGDDYEVDEKKRTVGILEPGVDKVERALGLENLYSA
                     ENTPLIAFLSNALKAKELFKRDKEYVVLNGEVDIVDEHTGRLLKGRRYSEGLHQALEA
                     KEGVPVKAENQTFASITLQNYFRLYTKLAGMTGTAQTEASELNGTYGMNVVPIPTNVP
                     MIRIDQPDYLYKNEQAKYDAVVEDIVERHAAGQPVLAGTTSVEKSEKLSKELKKHGIA
                     HEVLNAKEHEREAAIVAEAGRKGAVTVATNMAGRGTDIMLGGNPEFRAIGVMAKKGFD
                     LHEDPATYEANWAAVFEAEKKAVAKEHDEVVAAGGLYVLGTERHESRRIDNQLRGRSG
                     RQGDPGESRFYLSLEDELMRLFQTGLASAMMNSSAFPDDTPIESKVLTRGVRSAQAQI
                     EGRNHEARKAILKYDDVMSVQRKVIYAERRSILEGEDFGEQMRSFINDVIDERVSEVT
                     STGNTDDWDIPGLIKELLQVYPVSLTADEILDEVGGSVGLTYGYLLREFQSDAQLRYT
                     EVEDRIGVDVMRQVERQVVLQVLDNKWRDHLYEMDYLREGIGLRAMGQRDPLMEYQRE
                     GAQMFDAVSDGIKEQSVQVLFRVERRDPNEVQGQAPAGGPVNAASAAATAASPAGQAA
                     AVQTASGQTMAGPQAGAAFGVGRRIPTGPASTTGGPGGVQVSQMTYSGPTLDTASGKT
                     EAKKTKTSPSASGLEYPGTPRNAPCPCGSGKKYKLCHGKDAG"
     gene            complement(4753..5442)
                     /gene="raiA"
                     /locus_tag="VF362_01400"
     CDS             complement(4753..5442)
                     /gene="raiA"
                     /locus_tag="VF362_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX7589616.1"
                     /translation="MDIHVVGRHTKVAEEFRAKVVEKLGKIETLDPSATRIDVHVVHE
                     RSPHMVAERERIELTVHGRGPVIRTEAAAEDRVVALELAFDRLVERIRKLHERRAHRH
                     QGKVGVGHAAGLTVVSEPHDGPGERAAVSVEPWEGAPEGTTTEIPLAGTPIIIRSKTH
                     SGVPMTTPDAIDQMELVGHDFFLFHDSESGLPSAVYRRRGWTYGVIHLEAGRQAAGGL
                     HSGRESIEESA"
     gene            complement(5621..5881)
                     /locus_tag="VF362_01405"
     CDS             complement(5621..5881)
                     /locus_tag="VF362_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589617.1"
                     /translation="MTTPAAGASSFAASSFGAVLAAVRQGQTVGAAARSLGLAPELAE
                     AMLDEAKRLGLAVSAREVCGTCSPASASSLCAGCPVLGRHEV"
     gene            complement(5878..7782)
                     /locus_tag="VF362_01410"
     CDS             complement(5878..7782)
                     /locus_tag="VF362_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEX7589618.1"
                     /translation="MTCPSCEPVRASGPLGDASVLIVGNPNVGKSTLFNALTGSHQRA
                     VNAPGTTLELTLGTWRLGGEEVGVADLPGTFSLDARSVDERAVVAALDGADPHAVVVV
                     MLDATALSRSLYLLAQVIGTGRPIVAALTMLDVARDRGLSIDGPTLARAIGLPVVTID
                     PRKRPGAAELAEPVSRALAAPHPAGGARRVGPFGEADVVRWFVWVEAVMSNLPPEPPR
                     RVTPSDRIDRVLLHPWVGVPVFLAVMWGLFEMCTRVAGPLITAIAAFAGGPVAHAVSS
                     GLGALGVGGGWFEGLLVDGVLTGLGVVLSFLPLLALVFTALGILDGSGYLSRAAVVAD
                     RAMRVIGLDGRAVLPLMIGFGCNVPAIEATRVLPHARQRLVAGLLVPYTSCSARLTVY
                     LLLAAAFFPGSAGTVIFGLYLTSILLVAVGGLAFRKLVGGKGEPEREALVLVLPPYQW
                     PDLRTLWHSIAERVRGFVKEAGAVIVIALVAMWALAAVPVRGGYALADVPIADSVFGV
                     GARAVAPVLAPLGLGDWHVAAALASGFVAKEVVVGALAETYAVDGKGGAPALTARLNE
                     TLSETSGGHPRAAALAFMVFALAYTPCLATVAQMGRALGRRWTTIGIGLQLVVAWVLG
                     TLVFQVGRLL"
     gene            complement(7779..8000)
                     /locus_tag="VF362_01415"
     CDS             complement(7779..8000)
                     /locus_tag="VF362_01415"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEX7589619.1"
                     /translation="MYLAEVAPGRSVVIGALAGSPEAANRLREIGLRPGVSVRVLQRS
                     AFGAVVVAHGFSSVALDRETARSIAVESQ"
     gene            complement(8033..8758)
                     /locus_tag="VF362_01420"
     CDS             complement(8033..8758)
                     /locus_tag="VF362_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX7589620.1"
                     /translation="MDASREPGTLAVVAAAVARAIVPVVCPGCGLPDVRWCGDCAAVW
                     WEPPYRSESGAGRLDVLGRAPLPAWSIVPLVGPPHLMIGAWKDGGRRDLDPFFRDAMI
                     RAVASLAGALEPITVVVPVPARPASTRRRGVDLPGLLADAAASALARTNGSVLAARCL
                     RNSGREARGLGSRARWSGSAIGLDPGSAPAGRTALLVDDVVTTGASLARSCEALERAG
                     VAVAAAVTLAATLPPGTNRDLGP"
     gene            complement(8908..>10198)
                     /locus_tag="VF362_01425"
     CDS             complement(8908..>10198)
                     /locus_tag="VF362_01425"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEX7589621.1"
                     /translation="QDATGKWRISGIDSGIILAGAHFDDLFRPVEIAFASADGKVVVP
                     DLRWVPRSNSATFAANALLGGPSAWLADAVRTGIPVTAALTLQAVPITDGRALVALDT
                     DAGGNDAQRALARLQFAKTLTQLPDITSVEVTIGGLVTEGDGTVQLDDAPIPGTTAVA
                     FVNGRLGTWAADALTVVPPGAGALPAGANSPALSYDGKTVAFLIGSSQLVTTDALAAG
                     TVALDPAGAAPEGVMPVTVAYSGSNLTMPSFDWYGWVWTAERSGSGSLVALAPGAAPK
                     AVSVPWLEGREVGAVRVSHDGARVAVLSREGGVWRLDVAGLIRDADGMPLSVGQPLSV
                     GLGVGPASHLVWVDGRTLAVLADGPAGSTPSLGISVVGGGTTVLSSSADAIEIAARNR
                     TSSIAVVTRQGEIFQSSTAGWSKVPLPGAVEGLAFSG"
BASE COUNT         1606 a         3584 c         3539 g         1469 t
ORIGIN      
        1 cgccccacgg caccgtcgtc gccggaacat agatgcccga gccgacggcc acgtgtatca
       61 ccacgacgtc ggacagttct aggaccgttc cgggcgtgag ggtgtggcgc gccgcgtagt
      121 agggctccgt ctggtcggcg cgcgccacga cggacgccgt tccgagaatc gcgatcgcga
      181 tgagcgcaac gccgatcaac agccgcggat ccgaccagcg cgggcgcttg aggcgccccg
      241 cgatcgggcc aggggatgcg ttcatgacca cctccgatct tcggtggggc tatagtgcct
      301 cgtcgcgacg agtctcgctt ggtgatgtgg ataaccgggg gttcgcttgc tcgcgtggga
      361 gaatcgagca tgcctcccag gttcctcacg ctcgccgatg tggaagaggt actcaacatc
      421 acggcgaccc aggcatacgc gctcgtgcgc tcgggagacc ttcccgcgat ccaggtgggg
      481 ggacgcggcc aatggcgcat cgagtcctcg gaactcgagg cctatatcca gcgccagtac
      541 gaggcgactc gggcgaggat cacgacggag cgctagcgcg agaggctagg ctgcggcgcc
      601 ggatgacgca atcaccagca cctctgcgag cgatacggcc acggcgacgc cgtcgtccat
      661 cgcgagatcg aggtggtccg caccaacctg atcgatgcgc cccctgatct cgcctcccgt
      721 ggtcgagatg acgacgggca cgccctcgcg ggcgagcgcc ctgagggcat gaccgagccc
      781 gagccgcgcc gtcacggcgc cgaagcttga cgcgcggtgc cccacccccc gagccatcgc
      841 gatcgcgccc cgcggcacca gatactcgcg ccctccctcg gagagaagaa cccaggaacc
      901 cgcggcttcg gtcaccaagc ccgagacgtg ggagccgccg cgaagggtga gggtgagggt
      961 gacgggcgcg gacgccctcg acgcgaggcg ctcggccagt cgaacggtcg cgcgctcggc
     1021 ctgcgccagg tcccgaacac ccgcctcgaa ctccgacgcg tgcgcggcag agagctgccc
     1081 ctccaagtca ccgaacaagt cgttccaccg cattccccca acctacggta gccccgtcgt
     1141 ccacaaccgt ccccgttccg ctggacaatt aagcgatgtt gtactacccc tacttatatc
     1201 aaacagcatc aaccgatatc tagtgaggtc aagatgaccc ggaggggccg cgaccttgga
     1261 gggggtttgc accatgagcg cacaacttgc cgagatgccc gtcgccgggc tggtcgccgt
     1321 tccgtttccg gggccgcggc ctccgctcaa tccatacacg cgtccgcgca aggtcctagg
     1381 cgacccgacg ccactcgcat gcacggtcgc taaggtcgcg cttgaggtcg tgctcggggg
     1441 cgcggggctc gatcagttga cgcgatgggt cacgccggac attcgcgagg cgctcgcgca
     1501 gcagggcgcc ctagccagaa gggccggcct gaggatcggt gcccccgtgc gcatcctccg
     1561 gatacgggtc ttccgggtat ccgacgtcgc ggccgagacg agcgtcgtcg cggacgacgg
     1621 ggttcacgcg cgggcgatcg cggcacgctt cgaggacgtg ggcggacggt ggcaggccac
     1681 cgtcctcgac gtgggttgac tcaacgcctc agctagcggg gcgtggagac tagcggggtg
     1741 ccgccgcaag cgggacgcgg gcgcccccgc cgggagactt agcccgcgtc cttcccgtgg
     1801 cagagcttgt acttcttgcc cgagccgcaa gggcacgggg cgttgcgcgg cgtgccgggg
     1861 tactcgagcc cggaggccga gggcgaggtc ttcgtcttct tggcctccgt cttgccggac
     1921 gcggtgtcga gcgtcgggcc gctatacgtc atctgcgaca cctgaacccc gccgggtccg
     1981 cccgtcgtcg aagccggccc cgtgggaatg cgcctcccca caccgaacgc cgcccccgcc
     2041 tgggggccgg ccatggtctg acccgatgcg gtctgaacag cggccgcctg acccgccgga
     2101 gaggcggcgg tcgcggcggc cgaagcggcg ttgacaggtc cgccagcggg cgcctggccc
     2161 tgaacctcgt tcgggtcgcg acgctccacg cggaacagga cctggacgct ctgctccttg
     2221 atgccgtcgg agacggcgtc aaacatctgg gcgccctcgc gctggtactc catgagcggg
     2281 tcgcgctgac ccatggcccg caggccaatc ccctcgcgca ggtagtccat ctcatagagg
     2341 tggtcgcgcc acttgttgtc gagcacctgg agaaccacct ggcgctccac ctgccgcatg
     2401 acgtccactc cgatgcggtc ctccacctcg gtataccgca actgggcgtc cgactggaac
     2461 tcgcgcagca ggtacccgta cgtcaggcca acggagccgc caacctcgtc gagaatctcg
     2521 tccgccgtca gcgacaccgg gtagacctgg agcaactcct tgatgaggcc cgggatatcc
     2581 cagtcgtccg tgttgcccgt ggacgtcacc tcggagacgc gctcgtcgat cacgtcgttg
     2641 atgaaactgc gcatctgctc gccgaagtcc tcgccctcaa ggatgctgcg acgctccgcg
     2701 tagatcacct tgcgctgaac gctcatcacg tcgtcgtact tgaggatcgc cttgcgtgcc
     2761 tcgtggttgc ggccctcgat ctgcgcctgg gcgctccgca cgccgcgggt gaggaccttc
     2821 gactcgatgg gcgtgtcgtc ggggaaggcg gaggagttca tcatggcgga ggcgagcccg
     2881 gtctggaaca ggcgcatcag ctcgtcctcg agcgagaggt agaacctcga ctcgccgggg
     2941 tcgccctggc gaccggagcg gccgcgcaac tggttgtcga tgcggcgcga ctcgtggcgc
     3001 tccgtgccga gcacgtagag gccgccggcg gcgaccacct cgtcgtgctc cttggccacg
     3061 gccttcttct ccgcctcgaa cacggccgcc cagttggcct cgtacgtcgc gggatcctcg
     3121 tggaggtcga agcccttctt ggccatcacg ccgatcgccc tgaactccgg gttgccgccg
     3181 agcatgatgt ccgtgcctcg gccagccatg ttcgtcgcga cggtgaccgc gcccttgcgg
     3241 cccgcctccg cgacgatggc ggcctcgcgc tcgtgttcct tggcgttcaa gacctcgtgc
     3301 gcgatgccgt gcttcttcag ctccttggag agcttctcgg acttctcgac ggacgtggtt
     3361 cccgcgagca cgggctggcc cgccgcgtgc cgctcgacga tgtcctcgac gaccgcgtcg
     3421 tacttcgcct gctcgttctt gtacaggtag tcgggctggt cgatgcggat catcgggacg
     3481 ttggtgggga tcggaaccac gttcatgccg taggtgccgt tgagctccga cgcctcggtc
     3541 tgggccgttc ccgtcattcc cgcgagcttg gtgtagaggc ggaagtagtt ctggagcgtg
     3601 atcgatgcga acgtctggtt ctccgccttg accggcacgc cctccttcgc ctcgagcgcc
     3661 tggtggaggc cctccgagta gcggcgcccc ttgaggaggc gtcccgtgtg ctcgtcaacg
     3721 atgtcgacct cgccgttcag gacgacatac tccttgtcgc gcttgaagag ttccttcgcc
     3781 ttaagggcgt tggagaggaa ggcgatgagg ggggtgttct ccgcggagta gaggttctcc
     3841 aggccgagcg cccgctcgac cttgtccacg ccaggctcga ggatgccgac ggtgcgcttc
     3901 ttctcgtcca cctcgtagtc gtcgccggcc ttgagcgtgc ggaccagcgc ggcgaactcg
     3961 ccgtaccagc ggttgttcac gccctgggcc gggcccgaga tgatgagtgg cgtacgcgcc
     4021 tcgtcgatga ggatggagtc cacctcgtcg acgatcgcgt aaacgtggcc gcgctgcacc
     4081 agttcgccca tgctcatcgc catgttgtcg cgcaggtagt cgaagccgaa ctcgttgttc
     4141 gtgccgtagg tgacgtccgc gaggtactgc ttgcgccgct cctgcggctc catgccggtg
     4201 aggatgcaac cggtctcgag gccaaggaac ctgaacacgc ggcccatgag ctccgactgg
     4261 tagcgggcga ggtagtcgtt caccgtgacg atgtgaacgc ccaggccgtc gagcgcgttc
     4321 aggtacacgg ggagggtcgc gacgagcgtc ttgccctcgc cggtcttcat ctccgcgatg
     4381 ttgccccaat ggagcacggc gccacccatg agctggacgt cgaagtgcct caggcccaac
     4441 gtgcgacgcg aggcctcacg cacggccgcg aaggcctccg gcatcagcga gtcaagcgac
     4501 tccccgtccc gccgccgctg cttgaactcc tcggtcaggg aacggagctc gccgtcggac
     4561 atcgcctcgt aggcgctctc caggccgccc gcgatgcgcg cgacccgtgc gagccgacgc
     4621 aactctcggc cctcgcccat gcgcatcatg cggtcaatca gggacacggc aactccaaat
     4681 cggtcggtgg ctcggaaacc atcgtaggcg caaaggggcg gccgacgtgt gccggatgga
     4741 cgcgggaacc cgtcaggcgg attcctcgat ggactcgcgg ccggagtgga ggcccccggc
     4801 tgcctggcgt cccgcctcga ggtggatcac gccgtaggtc cagccgcggc ggcggtagac
     4861 cgcggacggc aagccgctct cggagtcgtg gaagaggaag aagtcgtggc caaccaactc
     4921 catctggtca atcgcgtcgg gcgtcgtcat gggtacgccg ctgtgggtct tgctgcggat
     4981 gatgatcggg gtgccagcga gcgggatttc cgtcgtcgta ccctcgggtg cgccctccca
     5041 tggctcgacg ctcacggccg cgcgctcgcc cgggccgtca tgaggctcac tcacgacggt
     5101 caggcccgcg gcgtgtccca cgcccacctt gccctggtgg cggtgggcgc ggcgctcgtg
     5161 cagtttgcgt atccgctcga cgagtcggtc gaaggccaac tcgagggcga cgacgcggtc
     5221 ctccgcggcg gcctcggtcc gaatgaccgg gccccttccg tgaacggtga gttcgatccg
     5281 ctcgcgctct gccaccatgt gggggctgcg ctcgtggaca acatggacgt cgatgcgcgt
     5341 cgcgctcgga tcaagggtct cgatcttccc cagcttctcg acgaccttgg cgcggaattc
     5401 ctccgcgacc ttggtgtggc gaccgacgac atgaatgtcc atggcaatcc tccaaaggtg
     5461 aggcggcagg gccgcaggtt gacgagggga gacgctgggt ctccggagtt ttcacctccc
     5521 tcgccacacg gagtacgacg cgaggcctcc gcttctctca ctctatgcca aggccccgac
     5581 gggcggcagg actgtctcgc tcttcacacc gcgtccctcg tcagacctcg tggcggccga
     5641 ggacggggca accggcgcac agggaagatg cggacgcggg ggagcaggtt ccgcacacct
     5701 cgcgcgccga gacggcgagt cccagcctct tcgcctcgtc gagcatggcc tcggcaagtt
     5761 cgggcgccag tccgagcgag cgcgcggccg cgccgacggt ctggccctgg cgcacggcgg
     5821 cgaggacggc cccaaaggaa gaggccgcga aggaggaggc acccgccgcg ggggtcgtca
     5881 cagcagcctc cccacctgga agaccaatgt cccgagcacc cacgccacga cgagttgcag
     5941 cccaatcccg atcgtggtcc agcggcgacc gagcgccctg cccatctgcg cgaccgtcgc
     6001 gaggcacggc gtgtacgcga gcgcgaagac catgaacgcg agggcggcgg cccgcgggtg
     6061 cccccccgac gtctccgaga gggtctcgtt caggcgcgca gtgagcgcgg gggcgccgcc
     6121 cttgccgtcc acggcgtacg tctccgcgag cgctcccacg acaacctcct tggcgacgaa
     6181 cccggacgcg agcgcagcgg cgacgtgcca gtcgcccagg ccgagcgggg ccagcaccgg
     6241 agcgacggcc cgcgccccca cgccgaacac gctgtcggcg atcggcacgt cggcaagggc
     6301 gtagccccca cgcacgggga ccgcggcgag cgcccacatc gccacgagcg cgatcacgat
     6361 gaccgccccc gcctccttca cgaatccacg gacgcgctcc gctatcgagt gccacagcgt
     6421 cctgaggtcg ggccactggt aggggggcag aacgaggacg agagcctcgc gctccggctc
     6481 gcccttgccc cccacgagct tgcggaaggc gagtcccccg acggcgacca gcaggatgct
     6541 cgtgaggtag agcccgaaga tgacggtccc cgcgctaccc gggaagaacg cggcggcgag
     6601 gagcaggtac acggtcagcc gcgccgagca cgacgtgtat gggaccagga gccccgcgac
     6661 gagccgctga cgggcgtggg gcaggacgcg cgtcgcctcg atcgcgggga cgttgcagcc
     6721 gaacccgatc atgaggggaa gcaccgcgcg gccatcgagc ccgatcacgc gcatcgcccg
     6781 gtcggcgacg acggcggcgc gagagaggta gccgctcccg tcgagaatcc cgagcgcggt
     6841 gaacacgagg gcaaggagcg gcaggaagga gagcacgacc ccgagccccg taagtacccc
     6901 gtcgacgagg agaccctcga accaccctcc ccccaccccg agcgcgccga ggcctgatga
     6961 cacggcatgg gcgaccggcc cgcccgcaaa cgccgcgatc gcggtgatga gcggtccagc
     7021 aacgcgggtg cacatctcaa agaggcccca catgacggcc aggaacacgg ggactcccac
     7081 ccaggggtgg agcagcacgc gatcgatgcg gtcggacggt gtgacgcgcc gcgggggctc
     7141 gggcgggagg ttcgacatca ctgcctcgac ccacacgaac cagcgcacga cgtccgcctc
     7201 gccgaagggg ccgacgcgcc gggcgccccc ggcggggtgg ggggcggcga gcgcccgcga
     7261 gaccggctcg gccagctcgg ccgctccggg gcgcttccgg ggatcgatgg tgacgacggg
     7321 cagcccgatc gcccgggcga gggttggccc gtcgatcgag aggcctcggt cgcgcgcgac
     7381 gtccagcatc gtcagggcgg cgacgatcgg cctgccggtg ccgatcacct gggcgagcag
     7441 gtagagggac cgagacagcg cggtcgcatc gagcatgacg acgaccacgg cgtgggggtc
     7501 ggctccgtcg agggcggcca cgacggcccg ctcatcgacg gaacgcgcgt ccaggctgaa
     7561 ggttccgggg aggtcggcga cgccgacctc ctcccccccg aggcgccacg tgccgagggt
     7621 gagttcgagg gttgtaccag gggcgttgac ggcgcgctgg tgggagcccg tgagggcgtt
     7681 gaagagggtt gacttgccga cgttcggatt gccgacgatc aggacggacg cgtcgcccag
     7741 cggtcccgat gctcgaacag gctcacacga gggacacgtc actgcgactc gaccgcgatg
     7801 gagcgcgctg tctcccggtc gagggccacg ctgctgaatc cgtgggccac cacgacggcc
     7861 ccgaatgccg agcgttggag gacgcggacg gacacccctg gtcggaggcc gatctcgcgc
     7921 aggcggttgg ccgcttccgg tgaacccgcg agcgcgccga tcacgacgct ccgccctggg
     7981 gccacctcag ccaagtacat gcatccagag tagaagtgag gtttacctaa cctcagggac
     8041 cgaggtcccg gttcgttccg ggcggcaggg tcgccgcgag ggtgaccgcg gcggccaccg
     8101 cgacgcccgc tcgctcgagc gcctcgcacg atcgcgcgag gctcgcgccc gtggtgacga
     8161 cgtcgtccac caggagggcc gtccgcccag cgggcgcgga gccggggtcg agaccgatgg
     8221 cggaaccgga ccatcgcgcg cgcgagccga ggccccgcgc ctcgcgcccc gagttcctca
     8281 ggcatctcgc ggcgagaacc gatccgttcg tccgggcgag ggccgaggcc gccgcatccg
     8341 cgagcagtcc agggaggtcg acgcctcgcc gcctggtgga cgcggggcgc gcgggaaccg
     8401 ggaccacgac ggtgatgggc tcgagcgccc cggcgagaga tgcgacggca cggatcatcg
     8461 cgtcccggaa gaagggatcc aggtcgcggc ggcctccgtc cttccacgcc ccgatcatga
     8521 ggtggggcgg cccgaccagg ggcacgatgg accaggcggg caagggcgcc cggccaagga
     8581 cgtcaagccg gcccgccccg gattcggacc gatagggggg ctcccaccag acggccgcgc
     8641 agtcgccgca ccaccgcacg tcggggagcc cgcaaccagg acagacgacc gggacgatcg
     8701 cgcgcgccac ggccgccgcg acgacggcga gggtccctgg ctctcgcgac gcgtccacga
     8761 gtgaaggatc gcgcccgccg cgggcgaggt gcgctgaggt cccgcggggg gcgggggcat
     8821 cgtccgcgcc ggaccctggg acatcggaca gcgggcatcg tccacgccgg accctgggac
     8881 atcggacagc ggagatcgga cggaccgcta gccggagaac gcgagcccct cgaccgctcc
     8941 ggggagagga acctttgacc accccgcggt cgaactctgg aagatctccc cctgcctggt
     9001 gaccacggcg atcgaacttg tccggttgcg cgcggcgatc tcgatggcat ccgccgacga
     9061 cgacaggacc gtcgttcctc cgccgaccac ggaaatcccg agggacggcg tcgagcccgc
     9121 cggaccgtct gccagcaccg cgagcgtccg cccgtcgacc cagacgaggt ggctcgcggg
     9181 cccgacgccc aggcccacgc tcagcggctg gcccacggat agtggcatcc cgtccgcgtc
     9241 gcgaatcagg ccagcgacgt cgagcctcca cacgccgccc tcgcgggaca ggacggcgac
     9301 cctcgccccg tcgtgcgaga cgcgaaccgc gccgacctcc cggccctcga gccacggcac
     9361 gctcaccgct ttgggtgccg cgccaggggc gagggccacg agggagccgc ttccggaccg
     9421 ttcggcggtc catacccagc cgtaccaatc gaagctaggc atcgtcaggt tgcttccgga
     9481 gtaggcgacc gtcaccggca tgacgccttc cggcgccgca cccgccgggt cgagggcgac
     9541 ggttcccgcg gcgagcgcgt ccgtcgtcac gagttgggac gacccgatca ggaacgcgac
     9601 cgtcttcccg tcgtacgaca gcgcgggcga gttggcgccc gcgggtagcg ctccagcgcc
     9661 cgggggaacg accgtcagcg cgtcggcagc ccaggttccc agtctcccgt tgacgaatgc
     9721 gacggccgtc gtcccgggga tcggggcatc atcgagttga accgtcccgt ccccctcggt
     9781 cacgaggcca ccgatcgtca cctccaccga cgtgatgtcg ggaagttgcg tgagggtctt
     9841 tgcgaactgc agccgggcga gcgcgcgttg cgcgtcgttc cctcccgcgt cggtgtcgag
     9901 cgcaaccaat gcccgaccgt cggtgatggg aaccgcctgg agggtcagag cggcggtgac
     9961 ggggatcccc gtccggacgg catccgccag ccacgccgac ggcccaccaa gaagcgcgtt
    10021 cgccgcgaag gtcgcggagt tgctgcgcgg gacccaccgc agatcgggga cgacgacctt
    10081 cccgtcggcc gacgcgaacg cgatctccac gggccggaac aggtcatcga agtgggcccc
    10141 cgcgaggatg atcccgctat cgattcccga gattcgccac ttgcccgtcg cgtcctgc
//
LOCUS       DASVKP010000067         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_924291, whole genome shotgun sequence.
ACCESSION   DASVKP010000067 DASVKP010000000
VERSION     DASVKP010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924291"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="VF362_01430"
     CDS             <1..1359
                     /locus_tag="VF362_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX7589622.1"
                     /translation="GMGFRDSNQDLLGFMHLVPDRARERIVDIASTQFEDGSAYHQYQ
                     PLTRRGNNAIGSGFNDDPMWLVLGTSAYIKETGDYSILDVMTPFDNDQSKATPPMDHL
                     KRSFDHVLNNLGPHGLPLIGRADWNDCLNLNCFSDTPGESFQITENKAGGTAESIFIA
                     GMFVGIGPDYIALATRRGDTPEAKRAEAEIAEMRETVLTHGWDGDWFLRAYDFFGTKI
                     GSKENDEGQIWIEPQGYCIMAGIGVEDGKAIKALDATRERMNTPHGIVLLNPAYTRYH
                     LEIGEVTSYPPGYKENAGIFCHNNPWIIIAETVVGRAEYAWEYYKQIAPAYREEISEV
                     HRLEPYVYAQMIAGKDAIRHGEAKNSWLTGTASWNFVAVSQFLLGVRPDWDGLIVDPC
                     LGPDLPEFTVTRKARGATYVISVKNSGGKGAKLTVDGVAIEGNQVPYAKPGSTVQVTA
                     TV"
     gene            1460..>1663
                     /locus_tag="VF362_01435"
     CDS             1460..>1663
                     /locus_tag="VF362_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018768808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX7589623.1"
                     /translation="MEVLHDRVDAGRRLAGALAHLKDEFPIVLGIPRGGVIVAAEIAK
                     ALEAPLGVLVVRKLGVPGREEFGF"
BASE COUNT          298 a          561 c          539 g          265 t
ORIGIN      
        1 ggcatgggtt tccgcgactc caaccaggac ctcctgggct tcatgcacct ggttcctgac
       61 cgcgcccgcg agcgcatcgt cgacatcgcg tcgacgcagt tcgaggacgg ctccgcgtat
      121 caccagtacc agccgctcac taggcgcggg aacaacgcca tcggctccgg cttcaacgat
      181 gacccgatgt ggctcgtcct cggcacgtcc gcgtacatca aggagacggg cgactactcg
      241 atcctcgacg tgatgacgcc gttcgacaac gaccagtcca aggcgacccc gcccatggac
      301 cacctgaagc gctccttcga ccacgtgctc aacaacctgg gcccgcacgg cctgccgctg
      361 atcggccgcg ccgactggaa cgactgcctc aacctcaact gcttctccga cacccccggt
      421 gagtccttcc agatcaccga gaacaaggcg ggcgggacgg ccgagtcgat cttcatcgct
      481 ggcatgttcg tcggcatcgg gccggactac atcgcgctcg cgacgcgtcg gggcgacacc
      541 cccgaggcga agcgcgccga ggccgagatc gccgagatgc gggagaccgt gctgacgcac
      601 ggctgggacg gggactggtt cctccgtgcg tacgatttct tcggcacgaa gatcgggtct
      661 aaggagaacg acgagggcca gatctggatc gagccgcagg gctactgcat catggctggc
      721 atcggcgtcg aggacggcaa ggcgatcaag gccctcgacg cgacgcgcga gcggatgaac
      781 accccacacg gcatcgtgct cctcaacccc gcatacacgc ggtaccacct ggagatcggc
      841 gaggtcacgt cctacccgcc cggctacaag gagaacgctg gcatcttctg ccacaacaac
      901 ccgtggatca tcatcgcgga gaccgtggtg ggtcgcgccg agtacgcctg ggagtactac
      961 aagcagatcg cgcccgccta ccgcgaggag atctccgagg ttcaccggct cgagccgtac
     1021 gtgtacgcgc agatgatcgc gggcaaggac gcgatccgtc acggcgaggc gaagaattcg
     1081 tggctcaccg gcacggcgtc gtggaacttc gtcgcggtgt cgcagttcct gcttggggtg
     1141 cgccccgact gggatggcct catcgtcgac ccctgcctcg gcccggatct gcccgagttc
     1201 accgtgaccc gcaaggcgcg tggcgcgacc tacgtcatct cggtgaagaa ctccggcggc
     1261 aagggcgcga agctcacggt cgacggggtc gcaattgagg gcaaccaggt tccgtacgcg
     1321 aagcctggct cgacggttca ggtgaccgcg accgtctgac cccgttcgcc ccgccttgga
     1381 ggtacggcta gctcctgtga gaaagtcgta cgccgcacct ccaaagcggg gcttcagacg
     1441 acggtcgtac cctggagtca tggaggtgct ccacgatcgc gtcgacgcgg gcaggaggct
     1501 ggccggggcg ctcgcccacc tgaaggacga gttcccgatc gtcctaggta tcccccgcgg
     1561 gggcgtcatc gtcgccgccg agatcgccaa agccttggag gccccgctcg gggtgctcgt
     1621 cgtgcgcaag ttaggggtgc ccggtcgtga ggagttcggg ttc
//
LOCUS       DASVKP010000068         3552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_843077, whole genome shotgun sequence.
ACCESSION   DASVKP010000068 DASVKP010000000
VERSION     DASVKP010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843077"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="VF362_01440"
     CDS             <1..1458
                     /locus_tag="VF362_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX7589624.1"
                     /translation="VIRLEQNYRSTQTILGAANAVIANNVGRVPKNLWTDAGAGNRIV
                     GYAGDTEQDEAQFVADEIARLVREDGVAPSDVAVMYRANAQSRSIEGRLAAADIPYVI
                     VGGTRFYDRREVKDMLAYLAAVVNRDDDIAMRRVINTPKRGIGDAAVEAIDALARGVG
                     MSFGAAVARLDEVAGLAPRSRAAIEGFARLMDDLRDLARDNGPASVLDAIADRSGMLA
                     ALRASDDPQDASRIENVMELVAVGREFGEENPAGTLSEFLEKVALVADADSLPAADGS
                     GGVVTLMTLHTAKGLEYPVVFVTGLEEGTFPHMRSLESVQDLEEERRLAYVGLTRARE
                     RLYLTRAEVRSSWGAPQYFSASRFAEEIPADLVEWRRSGTSTASLRAKADRYGSRAGE
                     WSSTYGARSGGDDDVYSRAGAATTRRIPPSPPPGGTVSSGLGLEVGDRVSHDQYGLGT
                     VVVLEGDGKNAVAKVDFGSAGVKRLLLRFAPMTKL"
     gene            1553..2545
                     /locus_tag="VF362_01445"
     CDS             1553..2545
                     /locus_tag="VF362_01445"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEX7589625.1"
                     /translation="MQTSGNKVAIVGAGSVGATLAYAGLMRGFARTVALYDINAAKVE
                     AEVLDLAHGVQFMPQANVIGSDQVDVCAGADVVVITAGAKQQPGQSRQDLAGATIGLM
                     DRIIPPLLAVAPEAIFILVTNPVDVVTYAAIKKSGYEARKMFGSGTVLDSSRLRYRVA
                     QECGVAIANVHAYIAGEHGDSEVALWSSASIGGLPLTEFRGSDGAVIMTPEKCEQIQR
                     EVKESAYKIIEGKGATNYAIGVAGARIVEAVLQDQNRVLPVSWLIEFPGVGEVCMSMP
                     AVVGRGGVSYRVNVPLNGEEHAALKASARSIREVAERFGAGYLGKKPAKTSKSA"
     gene            complement(2503..3285)
                     /locus_tag="VF362_01450"
     CDS             complement(2503..3285)
                     /locus_tag="VF362_01450"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HEX7589626.1"
                     /translation="MTTPPSSLPPANWYPDPQTPGQLRYWDGQRWTQHVHPAQQAAQG
                     VGGAGAAQVQAGPGVFLPSGRPGSAQPGQGGAAVQGGFTPVQAGPSQGGFSPTQAAPL
                     AVGPSTGAQRTFQPGGAVAASPFPPTMQNPTSLDVDDIPETRGAKRARDPYDSVESTG
                     SSTASKFASLFGFLALLATAASVYGLGTDFDAYVTYAAGGLGFVAVLLGFAGVGKAVT
                     GEGGRGRSILAICLGFLAIGLATYEYLNPHALFDVFAGFFPK"
BASE COUNT          557 a         1183 c         1263 g          549 t
ORIGIN      
        1 gtcatccggt tggagcagaa ctaccggtcc acccagacga ttctgggtgc cgcgaacgcc
       61 gtgatcgcga acaacgtcgg ccgggtccca aagaacttgt ggacggacgc cggagcgggc
      121 aaccgcatcg tcggctacgc cggggatacc gagcaggacg aggctcagtt cgttgccgac
      181 gagatcgcgc gcctcgtccg cgaggacggg gtggcgccgt ccgatgtcgc cgtgatgtac
      241 cgcgcgaacg cccaatcgcg atccatcgag ggccgactcg ccgctgccga catcccatac
      301 gtgatcgtgg gcgggacacg cttctacgac cgccgcgagg tcaaggacat gctcgcctac
      361 ctcgccgcgg tggtgaaccg cgacgacgac atcgccatgc gtcgcgtgat caacacaccc
      421 aagcgcggga tcggtgacgc ggcggtcgag gcgatcgacg cgctcgcgcg aggcgtgggg
      481 atgtcgttcg gcgcggcggt cgcgcgtctc gatgaggtcg cgggccttgc gccgcgctcc
      541 cgcgccgcca tcgagggctt cgctcgcctc atggacgacc ttcgcgacct tgctcgggac
      601 aacggccccg cgagtgtgct ggacgcgatc gcggatcggt ccggcatgct cgccgcactc
      661 cgcgcgagcg acgacccgca ggacgccagc aggatcgaga atgtgatgga actcgtggcc
      721 gtggggcggg agttcggtga ggagaacccc gccgggacgc tttccgagtt cctcgagaag
      781 gtcgcgctgg tcgccgacgc cgactccctc cccgccgccg atggctccgg tggagtcgtc
      841 accctgatga cccttcacac cgcgaagggg ctcgaatacc cggtcgtctt cgtcacggga
      901 ctggaagagg gaaccttccc tcacatgcgc tcgctcgagt cggtccagga cctcgaggag
      961 gagcgtcgcc tcgcctacgt tggcctgacg cgggcgcgcg agcgcctcta cctcacccgt
     1021 gcggaggtcc gctcgagttg gggcgcgccg cagtacttct cggcctccag gttcgcggag
     1081 gagatcccgg cggacctcgt cgagtggcgc cgctccggga cgtcaacggc ctcgcttcgc
     1141 gccaaggcgg atcggtatgg ctcgcgggca ggcgaatggt ccagcacgta cggcgcacgt
     1201 tccggcgggg acgacgacgt ctactcgcgc gccggggcgg ccacgacgcg caggatcccg
     1261 ccgtctccgc cgcccggcgg cacggtttcg tccggcttgg ggctcgaggt tggcgaccgg
     1321 gtcagccacg atcagtacgg gctcggtacc gtcgtcgtcc tcgagggcga tgggaagaac
     1381 gcggtcgcca aggtggactt cgggagcgcg ggggtcaagc gcctcctcct gcgcttcgct
     1441 cccatgacga agctctagtt gctggaggga cctgcacggg ctccgcgcgg cggcaactag
     1501 gctggtgcca ttcggcggag ggccgaactc ctggagcgac accaagggaa acatgcaaac
     1561 gtcgggcaac aaggtagcaa tcgtgggcgc gggctccgtc ggcgcgacgc tcgcctatgc
     1621 gggcctgatg cgcggattcg cgcgtacggt cgcgctctac gacatcaacg ccgcgaaggt
     1681 cgaggccgag gtcctcgacc tcgcgcacgg cgtccagttc atgccgcagg cgaatgtgat
     1741 cggatcggat caggtcgatg tctgcgcggg cgccgacgtc gtggtgatca cggcgggcgc
     1801 gaagcaacag cccggccaga gccgacagga tctcgctggg gcgacgatcg gcctcatgga
     1861 ccggatcatc ccgccgctgc tcgcggtggc gcccgaggcg atcttcattc tcgtaacgaa
     1921 ccccgtcgac gttgtcacgt acgcggcgat caagaagtcg gggtacgagg ctcggaagat
     1981 gttcggctcg ggcaccgtcc tcgactcgtc tcgactccgc tacagggtgg cgcaggagtg
     2041 cggggtcgcc atcgcgaacg ttcacgcgta catcgccggg gagcacggcg actccgaggt
     2101 cgcgttgtgg tcctccgcgt ccatcggtgg ccttcccctg accgagttcc gtgggtcgga
     2161 cggcgccgtc atcatgacgc ccgagaagtg cgagcagatc cagcgcgagg tcaaggagtc
     2221 ggcgtacaag atcatcgagg gcaagggtgc caccaactac gccatcggcg tcgccggggc
     2281 gcggatcgtc gaggcggtcc tccaggatca gaaccgcgtg cttccggttt cgtggctgat
     2341 cgagttcccc ggcgtgggcg aggtttgcat gtccatgccg gcggtcgtcg gtcgcggggg
     2401 ggtgagctat cgcgtcaacg tccctctcaa cggcgaggaa cacgccgcgc tcaaggcatc
     2461 cgcgaggtcg atccgggagg tggccgagag gttcggcgcg ggctacttgg ggaagaagcc
     2521 cgcgaagacg tcgaaaagcg cgtgaggatt gaggtactcg taagtggcga gcccgatcgc
     2581 caggaatccc aagcagatcg cgaggattga cctcccgcgc ccaccctcgc ctgtgaccgc
     2641 cttcccgacg ccggcgaatc cgagaagcac cgcgacgaac ccgagacctc ctgcggcata
     2701 cgtcacgtag gcgtcgaagt ccgtgccgag accgtacacc gaggccgcgg ttgccagcag
     2761 tgcgaggaat ccgaagaggc tcgcgaactt ggatgcggtc gaggacccgg tcgactcgac
     2821 cgagtcgtag gggtcgcggg cccgcttggc cccgcgcgtt tccggaatgt cgtccacgtc
     2881 gaggctcgtg gggttctgca tggttggcgg gaacggcgac gccgcgacgg cacctccggg
     2941 ctggaaggtc cgctgcgccc ccgtcgatgg gccgacggcg aggggagccg cctgcgtcgg
     3001 ggagaagccg ccctgggacg ggcccgcttg cacgggggtg aagccgccct gaactgccgc
     3061 gcctccctgg ccgggttgtg cgctacccgg tcggccgctc ggaaggaaca cgccgggccc
     3121 cgcctgtacc tgagcggctc ccgctccgcc aaccccttgg gcggcctgtt gtgcggggtg
     3181 gacgtgctgg gtccagcgct gcccatccca gtagcgcaat tggcccggag tctgggggtc
     3241 cgggtaccag ttcgccggcg gtagcgacga cgggggcgtt gtcatgatct ctccaaagtg
     3301 ggcactccca atcttgccct atcgcgacga taccggagtg atgagccgac acggcgcctc
     3361 ggcgctcgcg gcgcctcctc atgctcgatg ctagcgtccg cgcgcgccgg gcgcccgacg
     3421 aacacgagga agggccgggg caggcactcg ccccggccct tcccctcctc cctagaccag
     3481 tgctggctcg agttcaacct cgcgggtgcg ttccgtgcga ccctcgaaac tcagggcggg
     3541 gagccacgag ag
//
LOCUS       DASVKP010000069         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1007980, whole genome shotgun sequence.
ACCESSION   DASVKP010000069 DASVKP010000000
VERSION     DASVKP010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007980"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VF362_01455"
     CDS             complement(<1..211)
                     /locus_tag="VF362_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX7589627.1"
                     /translation="MSGPRLRVGVLALQGDVREHLAVLDACGAAGFTVRRLEELASAD
                     AIILPGGESTTIDKLLRAFGLFEPLR"
     gene            complement(208..708)
                     /locus_tag="VF362_01460"
     CDS             complement(208..708)
                     /locus_tag="VF362_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7589628.1"
                     /translation="MGAEEATPREAARVLVFDGSDRLLLLRGHDPVMPERSWWFTPGG
                     GLHEGETARQAASREAREETGFVIAPDELVGPVWERTAVFDFMERPYVQHEEFFVARV
                     GADSPREEAAWTAIERDTLEAVAWLSLDEIVGAEIEVFPNALGEILATVLPWDGVVRH
                     LGTEDA"
     gene            complement(712..1620)
                     /gene="pdxS"
                     /locus_tag="VF362_01465"
     CDS             complement(712..1620)
                     /gene="pdxS"
                     /locus_tag="VF362_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX7589629.1"
                     /translation="MASKDSASAPKTGTDLVKRGMAEQLKGGVIMDVVTADQAKIAED
                     AGAVAVMALERVPADIRAQGGVARMSDPDLIQGIIDAVSIPVMAKARIGHFVEAQVLQ
                     ALGVDYIDESEVLTPADYANHIDKWKFTVPFVCGATNLGEALRRITEGAAMIRSKGEA
                     GTGDVSNATTHMRSIRGAILRLTTLPEDELYVAAKELQAPYALVKEVARTGKLPVVMF
                     TAGGIATPADAAMMMQLGAEGVFVGSGIFKSGNPAERAAAIVKATTFFDDPKVIADVS
                     RGLGEAMVGINVEDVPEPHRLAERGW"
     gene            complement(1641..>2800)
                     /locus_tag="VF362_01470"
     CDS             complement(1641..>2800)
                     /locus_tag="VF362_01470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7589630.1"
                     /translation="GLAHAAAVRVGRMRIGIVCPYALDRPGGVQLHVLDLASALTDLG
                     HDVRILAPAAPNFPLPANVDNAGNSIPVPYNGSTARLRFGLMTALRVRRWLRDGHYDV
                     VHVHEPGAPSLSLLAMWTRNGPMVATFHTSNDSSAIMRMAKPFVKPGMEVLDARIAVS
                     PSADRTVKDHFRLDATHIIPNGVDTAAYIEAHPRPAWMGTSDEPTVGILGRLDEPRKG
                     LDDFLEMIPLVRAEVPGARFLAAGRYSARLAGKIRAAGAEAIGTLDEYQKQRFMASVD
                     IYCAPNTGGESFGIVLVEAMAAGAAVVASDLTAFRDVGTSLGPEPCIALHKVRDPADM
                     ARTIIRLLVNPAERHALALRGQETASHFDWTEVAPRVEATYREAIRMGASR"
BASE COUNT          414 a          973 c          987 g          426 t
ORIGIN      
        1 cgcgcagggg ttcgaacagc ccgaacgccc tgagtagctt gtcgatcgtc gtggattctc
       61 cgccgggcag gatgatcgcg tcggcggagg caagctcctc gagacggcgg accgtgaagc
      121 ccgcggcgcc gcacgcgtcg agcacggcca ggtgctcgcg cacgtcgccc tgaagggcga
      181 gcacgcccac gcggagccgg gggccgctca cgcgtcctcc gtcccgaggt gccgaaccac
      241 gccgtcccaa ggcaggacgg tcgcaaggat ctccccaagc gcgttcggga agacctcgat
      301 ctccgcgcca acgatctcgt cgaggctcaa ccacgcgacg gcctcgaggg tgtcgcgctc
      361 gatggccgtc cacgccgcct cctcgcgggg cgagtccgcg cccacccgcg cgacgaagaa
      421 ctcctcgtgc tgcacgtagg gccgctccat gaagtcgaac accgccgtcc gctcccagac
      481 aggcccaacc agctcgtcgg gcgcgatgac gaagcccgtc tcttcgcgag cctcgcgaga
      541 ggccgcctgt cgcgccgtct cgccctcgtg aaggccgcca cccggcgtga accaccagga
      601 ccgttccggc atcaccgggt cgtgcccgcg caacagcagg aggcggtcgg acccgtcaaa
      661 caccaggacg cgggcggcct cccggggagt cgcctcctcg gcgcccatgc tctaccagcc
      721 gcgctcggcg agtcggtgag gctcgggcac gtcctcgacg ttgatcccga ccatcgcctc
      781 gcccagcccg cgcgagacgt ccgcgatgac cttcggatcg tcgaagaacg tggtcgcctt
      841 gacgatcgca gcggcgcgct cggcggggtt tcccgacttg aagatcccgg agccgacgaa
      901 cacgccctcg gcgccgagtt gcatcatcat cgcggcgtcg gcgggggtgg cgatgcctcc
      961 cgcggtgaac atgacgacgg ggagtttgcc ggtccgcgcg acctccttga cgagggcata
     1021 cggggcctgc aactccttgg ccgcgacgta caactcgtcc tccggcaggg tcgtgagcct
     1081 caggatcgcc ccccggatgg agcgcatgtg ggtggtcgcg ttggagacgt cgccggtgcc
     1141 cgcctcgccc ttggagcgga tcatcgccgc gccctcggtg atgcggcgga gcgcctcgcc
     1201 caggttggtc gccccgcaca cgaagggaac ggtgaacttc cacttgtcga tgtggttcgc
     1261 gtagtcggcg ggcgtgagca cctcggactc gtcgatgtag tcaacgccga gcgcctggag
     1321 cacctgcgcc tcgacgaagt ggccgatgcg cgccttcgcc atcaccggga tggacacggc
     1381 gtcgatgatg ccctggatca ggtcggggtc gctcatgcgg gccacgccgc cctgggcgcg
     1441 gatgtcggcg gggacccgct caagggccat gacggcgacc gccccggcgt cctcggcgat
     1501 cttcgcctgg tcggccgtca ccacatccat gatgacgccg cccttgagtt gctcggccat
     1561 gccgcgcttg accaggtcgg tgccggtctt cggtgcgctt gcgctgtcct tcgatgccat
     1621 ggtgactccc ccggtgcctg tcagcgtgaa gcacccattc taatcgcctc gcgataggtg
     1681 gcctcgacgc gaggggcaac ctcggtccag tcgaagtgcg aggcggtctc ctgaccgcgc
     1741 agggcgaggg cgtgccgttc cgccgggttc accagcaggc ggatgatggt gcgggccatg
     1801 tccgcggggt cgcggacctt gtgcagggcg atgcagggct ccggccccag cgaggtgcca
     1861 acgtcccgga acgccgtgag gtcgctcgcc acgacggcgg ctcctgcggc catggcctcg
     1921 accagcacga tgccgaagct ctcgcccccg gtgttgggcg cgcagtagat gtcgaccgag
     1981 gccatgaagc gctgcttctg gtactcgtcg agcgtgccga tcgcctcggc ccccgcggca
     2041 cggatcttgc ctgcgagcct cgccgaatag cgacccgcgg cgaggaaccg tgcccccggc
     2101 acctcggccc gcacgagcgg gatcatctcc aggaagtcgt cgagcccctt gcgcggctcg
     2161 tcgaggcgtc caaggatccc caccgtcggc tcgtccgatg tgcccatcca cgcgggccgg
     2221 gggtgggcct cgatgtaggc ggccgtgtca acgccgttgg ggatgatgtg ggtcgcgtcc
     2281 aggcggaagt ggtccttgac ggtccggtcg gccgagggcg agacggcaat gcgggcatcg
     2341 aggacctcca tccccggctt gacgaagggc ttcgccatcc gcatgatcgc ggacgagtcg
     2401 ttggaggtgt ggaaggtcgc gaccatgggg ccgttgcgcg tccacatggc caggagcgag
     2461 agcgagggcg ccccgggttc gtggacgtgg accacgtcgt agtggccgtc gcgcaaccag
     2521 cggcgaacgc gcagcgccgt catcaggccg aaccgcagcc gcgcggtcga gccgttgtac
     2581 ggcacgggga tggaattgcc ggcgttgtcc acgttggccg ggagggggaa gttcggcgcg
     2641 gccggagcaa ggatgcgcac gtcgtgcccg aggtcggtca gcgcggacgc gaggtcgagg
     2701 acgtggagtt gcacgccgcc cgggcggtcg agggcgtatg ggcaaacgat tccgatcctc
     2761 attcgaccca cccgaaccgc tgcagcatgt gccagtcctc
//
LOCUS       DASVKP010000070         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_843625, whole genome shotgun sequence.
ACCESSION   DASVKP010000070 DASVKP010000000
VERSION     DASVKP010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843625"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VF362_01475"
     CDS             complement(<1..841)
                     /locus_tag="VF362_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX7589631.1"
                     /translation="MSTDLVTEEGGARSGFSVHLDNFEGPFDLLLSLISKHQMEITDV
                     SLARVTDEFIATVMNTDWDLSGASEFLVVAATLLDLKAARLLPQGEVEDAEDIEALEA
                     RDLLFARLLQYRAFKDVAASIELMLASPRRMPRNVPLEQHLAALLPELVWAVDPEGLA
                     RLAAHALRPRSPAEISLGHLHVTKVSVREQAALITSILQREGTVTFRALVSDTRDVSV
                     VVARFLALLELFRESVVAFDQVRSLSDLTVRWTGGDEAVEVVTDFDDEIHDDEIHGDG
                     THND"
     gene            complement(853..1668)
                     /locus_tag="VF362_01480"
     CDS             complement(853..1668)
                     /locus_tag="VF362_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7589632.1"
                     /translation="MTTFPVPAPLKAHGPARVIALCNQKGGVGKTTTAVNLAAAFAEL
                     GRKVLLVDFDPQGAVGAALGVNPHGLDRTIYDVLMKDDVDARDAIISGGVENLDVLPA
                     NIDLSAVELQLVSEVAREGALDRALTPVRPDYDLIFIDCQPSLGLLTVNALVASDGVL
                     IPLECEFFAMRGVALLLDTIGKVRSRLNPKLVVDGILPTMWDPRTLHCREVVETMRGA
                     FTDQVTETMIGRTVKFPDATVAAESILTFAPDHAGAEAYRNLARELVARGAAA"
     gene            complement(1686..2621)
                     /locus_tag="VF362_01485"
     CDS             complement(1686..2621)
                     /locus_tag="VF362_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX7589633.1"
                     /translation="MLERQRDSYLAHVTVERGLSRNTISAYRRDLDRYVAFLSGRGIA
                     SLADVTKAEVSAFSQAVGTGSDGGTALAPSSASRTIVAIRGWHRFALAEGWASDDPSH
                     EVRPRAIPRRLPKAITTEQVFAVLEAAGAGPEAEAPTVAQLRDRALLEFVYATGARIS
                     EAVGLDVDDLTLDVGEESVLLRGKGRKERVVPVGRIAAREVDAYLVRGRGALAAHGKG
                     TAAVFLNTRGARLSRQSAWSILKAAAERAGVPGVSPHTLRHSFATHMLSGGADVRVVQ
                     ELLGHASVTTTQVYTLVTRDSLREVYTMAHPRALG"
     gene            2803..>3539
                     /locus_tag="VF362_01490"
     CDS             2803..>3539
                     /locus_tag="VF362_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019175063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX7589634.1"
                     /translation="MSAILTDQDRAVVDTLKVLAADAVEKVGNGHPGTAISLAPAAYL
                     LFQKIMRHDPADPKWLGRDRFVLSAGHSSLTLYLQLFASGYDMTLDDLKALRTAGSRT
                     PGHPEYGHTAGVEITTGPLGSGFASAVGMAMAARRERGLFDPESAPGESLFDHRVFVI
                     ASDGDLQEGVSSEASSLAGTHELGNLVVLWDDNHISIEDDTSIAFSEDVLARYEAYGW
                     DVHRVSWIQKDGSYVEDLDALKAAYDAS"
BASE COUNT          550 a         1251 c         1205 g          533 t
ORIGIN      
        1 cgtcgttgtg agtcccgtcg ccgtgaatct cgtcgtcgtg aatctcatcg tcgaagtcgg
       61 tgacgacctc gaccgcctcg tccccgcccg tccaccgaac ggtgagatcg ctcagcgacc
      121 gtacctgatc gaaggcgacc acggattccc gaaacagttc caagagcgcg aggaaccgcg
      181 ccacgacgac cgatacgtcc cgagtgtcgg aaacgagggc gcgaaaggtg accgtcccct
      241 cgcgctgaag gatggacgtg atgagcgccg cctgctcgcg caccgagacc ttggtgacgt
      301 ggaggtgccc cagcgagatc tcggcgggcg atctcggccg gagcgcgtgg gccgcgagcc
      361 gcgcgagccc ctcggggtcc accgcccaca cgagttcggg caggagcgcg gcaagatgct
      421 gctcgagcgg cacgttgcgc ggcatgcgtc gaggtgaggc gagcatgagt tcgatgctcg
      481 cggcaacgtc cttgaatgcg cggtattgca ggagccgcgc gaagaggagg tccctggcct
      541 caagcgcctc gatgtcctcg gcgtcctcga cctcgccctg gggcaggagc cgcgccgcct
      601 tcagatcgag gagggtcgcg gcaacgacga ggaactcgct cgcgccgctc aggtcccagt
      661 cggtgttcat caccgtcgcg atgaactcgt ccgtgacgcg ggcgagggag acgtcggtga
      721 tctccatctg gtgcttcgag atgaggctga ggaggaggtc gaagggtccc tcgaagttgt
      781 cgaggtgcac cgagaagccg gatcgagccc cgccctcctc ggtcaccagg tccgtactca
      841 cgccgcccgc attcacgccg cggccccccg ggccacgagt tcgcgcgcga ggttgcggta
      901 cgcctccgcc cccgcgtgat cgggcgcgaa cgtgaggatg gactcggccg caaccgtcgc
      961 gtcggggaac ttcaccgttc gcccgatcat cgtctcggtg acctggtcgg tgaaggcgcc
     1021 gcgcattgtc tcaacgacct cccggcagtg gagcgtgcgc gggtcccaca tcgtcgggag
     1081 gatgccgtcg accacgagct tgggattgag gcgcgaccga accttgccga tcgtgtcgag
     1141 caggagcgcg acgccgcgca tcgcgaagaa ctcgcactcg agcgggatga ggacgccgtc
     1201 cgacgccacg agggcattga cggtcagcag gccgagcgag ggttgacagt cgatgaagat
     1261 caggtcgtag tccgggcgga cgggcgtcag ggcccggtcg agggcgccct cgcgggcgac
     1321 ttcggacacc agctgcaact ccacggcgga caggtcgatg ttcgccggca gcacgtcgag
     1381 gttctctacg ccgccggaga tgatcgcgtc gcgcgcgtca acgtcgtcct tcatcaggac
     1441 gtcgtagatg gtccggtcga ggccgtgcgg gttgacgcca agcgcggcgc ccacggcgcc
     1501 ctgcgggtcg aagtccacca gcaggacctt gcgccccagc tccgcgaacg ccgccgcgag
     1561 gttcaccgcc gtggtcgtct tgccgacgcc gcccttctgg ttgcacaggg cgatgaccct
     1621 ggccgggccg tgtgccttca ggggcgcggg cacggggaag gtggtcacga tgctcacgct
     1681 acccgctaac ccagggccct tgggtgggcc atcgtgtaca cctcgcggag gctgtcgcgg
     1741 gtgacgagcg tgtagacctg ggtggtcgtg acgctcgcat gtccgagcaa ctcctggacg
     1801 acgcgcacgt cggccccgcc ggagagcatg tgcgtcgcga aactgtggcg aagcgtgtgg
     1861 ggggaaacgc ccggcacgcc cgcccgctcc gccgccgcct tcaggatgct ccacgcgctc
     1921 tgcctgctga gcctcgcgcc gcgggtattg aggaagaccg cggccgtccc cttcccgtgc
     1981 gcggcgagag cgccccggcc gcgcaccagg taggcatcca cctcgcgcgc cgcgatgcgc
     2041 ccgaccggga cgacccgttc cttgcgcccc ttgcccctga ggaggacgga ctcctccccc
     2101 acatcgaggg tgaggtcatc gacgtcgagg cccaccgcct cgctgatgcg cgcgccggtc
     2161 gcgtagacga actccagcag cgccctgtcg cgaagttggg cgacggtcgg cgcctccgcc
     2221 tccggtccgg cgcccgccgc ctcgagcacg gcgaacacct gctccgtcgt gatggccttc
     2281 ggaagcctcc tcgggatggc ccgcggccgg acctcgtggg acggatcgtc ggaggcccag
     2341 ccttcggcga gcgcgaagcg atgccatccc cggatcgcga cgatcgtcct tgacgccgac
     2401 gacggcgcga gcgccgtccc cccgtcggag cccgtcccga cggcctggct gaacgccgag
     2461 acctccgcct tcgtgacgtc ggcgagcgag gcgatgcccc gcccggagag gaacgcgacg
     2521 tagcggtcga ggtcccgcct gtaggccgag atcgtgttgc ggctcagccc gcgctccacc
     2581 gtgacgtggg caaggtacga atcgcgctgc cgctcaagca ctacgggcgc cagacgatca
     2641 gcgcgtggta gaggaaccgc gcccccgcga ccgtgggggc aagcacggtg gcggacatgg
     2701 ctccatcgta gtcggggggt gcgatgcttc cccggtcctg cggcccccca cgtccggcgc
     2761 cgctaggcta tgagcatcat ccccgagcca cttggagacc ccgtgagcgc catcttgacc
     2821 gaccaggacc gggcggtcgt cgacaccctc aaggtgcttg ccgccgacgc cgtggagaag
     2881 gtgggcaatg ggcaccccgg caccgccatc agcctcgcgc ccgccgccta tctcctgttc
     2941 cagaagatca tgcgccacga ccccgccgac ccaaagtggc tcgggcgcga ccggttcgtc
     3001 ctctccgcgg gacactcctc gctgacgctg tacctgcagc tcttcgcctc cgggtacgac
     3061 atgaccctcg acgacctcaa ggcgcttcgc acggccggtt cgcgcacgcc cgggcacccc
     3121 gagtacggcc acaccgccgg cgtggagatc accaccggcc ccctcggctc ggggttcgcc
     3181 tccgccgtcg gcatggcgat ggccgcccga cgcgagcgcg gccttttcga ccccgagtcc
     3241 gcaccgggcg agagcctttt cgaccaccgc gtcttcgtca tcgcttcgga cggcgacctc
     3301 caggagggcg tatcgagcga ggcatcttca ctcgccggaa cgcacgaact gggcaacctc
     3361 gtcgtcctat gggacgacaa ccacatctcg atcgaggacg acacctcgat cgcgttctcc
     3421 gaggatgtcc tcgcgcggta tgaggcgtac gggtgggacg ttcaccgcgt gtcctggatc
     3481 cagaaggatg gctcgtacgt cgaggacctc gatgccctga aggccgccta cgacgcgtc
//
LOCUS       DASVKP010000071         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_925659, whole genome shotgun sequence.
ACCESSION   DASVKP010000071 DASVKP010000000
VERSION     DASVKP010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925659"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1996)
                     /locus_tag="VF362_01495"
     CDS             complement(<1..1996)
                     /locus_tag="VF362_01495"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX7589635.1"
                     /translation="MSQAAIENPVINSPYVEPVRHFKTTADGGVTGELIERRRPSEFF
                     VPVARPKKASAQLTLDAYGPPTRQQPNEIVNEIRGSVERWRLLGYPGVASVTRDLLAY
                     WRSEDRERRLFFCQLEAVETVIYLTEVAEKLGDTKAINVIRAANVDLNDGLHRLAVKM
                     ATGSGKTVVMAMLIGWQALNKLTSPQDRRFADTFLVVTPGITIRDRLRVLLPNDPGNF
                     YRALDILTPENLDRLQAATIVITNFHAFIRRETVKAASLTTKVLAAGDSDAERFRETP
                     AEMVRRVCRVLGNAKNVVVLNDEAHHCYASAPHDEEGMLDPDERVEAKRNEEEARVWL
                     SGLRAVRDKIGIRSIYDLSATPFFLKGSGYAEGTLFPWVVSDFGLMDAIEAGIVKVPR
                     VPVSDDSMSGDSPAFRDLWLRVRDDLPKKGIRDTAIDGQPVIPKELEAALLTLYADYE
                     RSHGQWVTANMGRPPVFIVVCSNTSVSKLVFDWIAGWGKDLADGTTTIMSGNLSLFSN
                     ERDGRWLGRANTILVDSAQLDRGDALDPAFRKAAAREIEEFKREYMARFPGRSADDIT
                     DEDILREVMNTVGKHGKLGEDIRCVVSVSMLTEGWDANTVTHILGIRAFGTQLLCEQV
                     VGRALRRASYDVNPDGMFDPEYAEVYGVPFSFLSVAGRGRV"
BASE COUNT          328 a          645 c          696 g          367 t
ORIGIN      
        1 tgacgcgacc ccggcccgct acggacagga agctgaacgg gaccccgtac acctccgcgt
       61 actcgggatc gaacatgccg tctgggttga cgtcgtagct ggcgcgtcgg agcgcacgcc
      121 ccacgacctg ctcgcacagg agctgcgtgc cgaaggcccg gatgccgagg atgtgggtga
      181 cggtgttggc gtcccacccc tcggtgagca tcgagaccga gacgacacac cggatgtcct
      241 cgccaagctt gccgtgtttc ccgacggtgt tcatcacctc gcgcaggatg tcctcgtcgg
      301 tgatgtcgtc ggcgctgcga cccgggaacc tcgccatgta ctcgcgcttg aactcctcga
      361 tttcgcgagc cgctgccttg cggaacgcgg gatcgagggc atccccccga tcaagctgcg
      421 cggagtcgac gaggatcgtg ttcgctcgtc cgagccagcg tccgtcgcgt tcgttgctga
      481 agagcgacag gtttcccgac atgattgtcg tcgtgccgtc ggcgaggtcc ttcccccagc
      541 ccgcgatcca atcgaacacg agcttggaga cggatgtgtt cgagcagacc acgatgaaga
      601 cgggcggccg ccccatgttc gcggtgaccc attgcccatg cgaccgctcg tagtcggcat
      661 agagtgtcag gagggcggcc tccagttcct tggggatgac gggctggccg tcgatggcgg
      721 tgtcgcggat gcccttcttc gggaggtcgt cacggacccg cagccagagg tctcggaagg
      781 cgggcgagtc gccgctcatc gagtcgtcgg agaccgggac ccttggcacc ttgacgatcc
      841 cggcctcgat cgcgtccatc agcccgaagt cggagacaac ccacgggaag agcgtcccct
      901 cggcgtaccc cgagcccttc aggaagaacg gcgtggccga gagatcgtag atcgagcgga
      961 tgccgatctt gtcccggacg gcccggaggc ccgacagcca gacccgcgcc tcctcctcgt
     1021 tacgcttcgc ctcgacgcgc tcgtctgggt cgagcatgcc ctcctcatcg tgaggcgcgg
     1081 aggcgtagca gtgatgcgcc tcgtcattga ggacgacgac gttcttggcg ttgccgagga
     1141 cccgacagac tctccgcacc atctcggcgg gggtctcgcg gaaccgctcg gcgtcactgt
     1201 cgcctgctgc gagaaccttc gtggtgagcg aagctgcctt gaccgtctcg cggcggatga
     1261 aggcgtgaaa gttggtgatg acgatcgtcg cagcttggag ccggtcgagg ttttccgggg
     1321 tgaggatgtc gagggcgcgg tagaagttac ccggatcatt cgggaggaga acgcgcaagc
     1381 gatcgcggat cgtgatcccg ggcgtcacca cgaggaacgt gtcggcaaag cggcggtcct
     1441 gcggggaggt gagcttgttg agcgcctgcc acccgatgag catcgccatg acgaccgtct
     1501 tgcccgaccc ggtcgccatc ttgacggcga gtcgatgcag cccgtcattg aggtcgacgt
     1561 tcgccgcccg gatcacattg atggccttgg tgtcgccgag cttctcggcg acttcggtga
     1621 ggtagatgac ggtctcgacg gcttcgagct gacagaagaa gaggcgtcgc tcgcggtcct
     1681 cactcctcca gtaagcgagg agatcccggg tgacgctcgc cacgccggga tagccgagca
     1741 gtcgccaccg ctcgaccgag ccccggatct cgttgacgat ctcgttgggc tgctggcggg
     1801 tgggcggccc gtaggcgtcg agggtcagtt gggccgacgc cttcttgggg cgggcgaccg
     1861 ggacgaagaa ctccgatggt cggcggcgct cgatgagttc gccggttacg cccccgtcgg
     1921 cggtcgtctt gaaatgccgc accggctcga cgtacggcga gttgatgacc gggttctcga
     1981 tggccgcttg gctcacgaat gcgcctcggt ggggattagc cgtcaacctc ggaccc
//
LOCUS       DASVKP010000072         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_351875, whole genome shotgun sequence.
ACCESSION   DASVKP010000072 DASVKP010000000
VERSION     DASVKP010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351875"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VF362_01500"
     CDS             complement(<1..501)
                     /locus_tag="VF362_01500"
                     /inference="COORDINATES: protein motif:HMM:NF014754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX7589636.1"
                     /translation="MSGGSSRLRHWIDSGTRAVLRERDRLDAINVFPVADSDTGTNLY
                     LTLMEGSRAVARLPIDASHREVVAAFARGCLMGARGNSGVIVSQYLGAFLAEIDARGG
                     LDNVKGPGIVDAFDFAADAAYDAVGEPMEGTILSVARAAARGGRGALEAGGDVASIAV
                     AAVIEAR"
     gene            688..879
                     /gene="rpmB"
                     /locus_tag="VF362_01505"
     CDS             688..879
                     /gene="rpmB"
                     /locus_tag="VF362_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX7589637.1"
                     /translation="MAATCDVCGKSPSFGYSVSHSHRRTSRRWNPNIQRVRALVAGTP
                     KRLNVCASCLKAGKVTRRA"
     gene            complement(928..1887)
                     /locus_tag="VF362_01510"
     CDS             complement(928..1887)
                     /locus_tag="VF362_01510"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX7589638.1"
                     /translation="MPLIGDVGEDALIALFSPRLPTTTAALLGPGDDAAVLSLKGDLV
                     VSSDVLVQDRHFRREWSTAADVGYRAAMQNLADIDAMGAVPVALQVTLAAPPSTDVEW
                     VLEFADGLHEACEPLGVGVIGGDISGSREICVSITALGETRGRTPVLRSGARVGDVVA
                     VSGPLGAAMAGYHQLENGLRIDSESIDLFLRPQPRIGAGVEACDARATAMMDISDGLV
                     TDLARMALASGIGIAIESSRVPIHPSVARVAGQVVASPLDWALTGGEDHHLLATFPAH
                     AELPGGWVPIGLVTGGHEGIEVDGKAPTRWGWDHFPRAQGQSA"
     gene            2067..2693
                     /locus_tag="VF362_01515"
     CDS             2067..2693
                     /locus_tag="VF362_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589639.1"
                     /translation="MAWRDDVSEGAQSDLDTVFSQAALLARTLLMRNREFFPFGATIS
                     TKGAVGLSTPVPAAATPTVHDVIDAIKTELRHDKAGKRAYAIVALARTADGDEAVRIE
                     LEHKEGIALVIGMPVLRGESGTVTFGDMAAHPGQRLIWPPKAPAGAKGPATKAPAKAP
                     TRDPAKSSGLPAAAAASAAPKTASKRARTVAPKAKPARKPRGTAIATA"
BASE COUNT          467 a         1125 c         1038 g          415 t
ORIGIN      
        1 tctggcctcg atcacggcgg ccaccgcgat ggacgcgacg tctccgcccg cctcgagcgc
       61 gccgcgcccg ccccgcgccg ccgcccgcgc gaccgagagg atcgtcccct ccataggctc
      121 cccgacggcg tcgtaggccg cgtcggccgc gaagtcgaac gcatcgacga tgccgggccc
      181 cttgacgttg tcgagtccgc cccgcgcgtc gatctccgcc aggaaggcgc ccaggtactg
      241 ggagacgatc acgcccgagt tcccacgcgc gcccatgagg caaccccgcg cgaacgcggc
      301 caccacctcc cggtgactgg cgtcgatcgg aagtcgggcg acggccctgc tgccctccat
      361 gagggtgagg tagaggttgg tgccggtgtc agagtcggcg acggggaaga cgttgatcgc
      421 atccagtcgg tcgcgctcgc gcaggacggc gcgggtcccg gagtcgatcc agtggcgcag
      481 ccgcgacgat cctccgctca ccgcgcctcc tcgctcggtc aatcctccgt gcgcaccatc
      541 gtggcacgcc gacggcggca tgcgaaaccc ggccggggcg ggtttgtggt cagcgcccat
      601 agtcggctac tcttgtgcgg ttgcctgccc agcaggccct catcgtcggc gcggaacccg
      661 cgccccatgc aaggtcagga gaagatcgtg gctgctacct gcgacgtctg cggcaagagc
      721 ccgtcgttcg ggtactcggt ctcccattcc caccgccgca cctcgcgccg ctggaacccg
      781 aacatccagc gcgtgcgcgc gctcgtggcg ggaacgccca agcgcctcaa cgtgtgcgcc
      841 tcgtgcctca aggccggcaa ggtcacgcgc cgcgcctagt ggttccgccg ccacgtcgcg
      901 gcgaagctgt gcgtaggagc tgtgtgccta ggcgctctgc ccctgggcgc gagggaagtg
      961 atcccagccc caacgggtcg gagccttgcc gtccacctcg atgccctcat ggccaccggt
     1021 gacgagtccg atcggcaccc acccccccgg tagttccgcg tgcgccggga acgtcgccag
     1081 caggtggtga tcctcgcctc ccgtgagcgc ccagtccaag gggctcgcca cgacctggcc
     1141 cgccacgcgc gcgacggacg ggtggatcgg gacgcgggag gactcgatgg cgatcccgat
     1201 gccggacgca agcgccatgc gggcgaggtc ggtcaccagc ccgtcggaga tgtccatcat
     1261 cgcggttgcg cgagcgtcgc aggcctcgac gccggccccg atcctcggct gcgggcgcag
     1321 gaacaggtca atcgactccg agtcgatccg caggccgttc tcgagttggt ggtaccccgc
     1381 catcgcggcg ccgagcggtc ccgacacggc caccacgtcg cccacacgcg cgcccgaccg
     1441 aaggacgggc gtgcgtccgc gagtctcgcc gagggcggtg atgctgacgc aaatctcgcg
     1501 cgaacccgag atgtcgccgc cgatgacgcc gacgcccagc ggctcgcaag cctcgtgcag
     1561 gccgtccgcg aactccagca cccactccac gtccgtcgag gggggcgcgg cgagggtgac
     1621 ctggagcgcc accggaacgg cgcccatggc atcgatatcg gcgaggttct gcatcgccgc
     1681 gcggtagccg acgtcggccg ccgtcgacca ttccctcctg aagtgccggt cctgcaccag
     1741 gacgtcgctg gacacgacga ggtcgccctt gagggagagc accgcggcgt cgtcgccggg
     1801 gcccaggagg gctgcggtcg tggtgggaag acgcggggag aagagcgcga tgagcgcgtc
     1861 ctccccgaca tccccgatga ggggcatggc accagggtaa ggggctttcg cctcgggcgg
     1921 gggccggttc cccgtatgcg gcgaaagtcg cgagcgcgcc cattgtcccg ccttgggcgg
     1981 cccgcgaaac gcgcggcgcg gcatccgggg ggcctcgccg ctcgcggcta ccgtggtgag
     2041 gaagccgaag gagagcgagg cctcgtatgg catggcggga cgacgtttcc gagggagctc
     2101 agagcgacct cgacacggta ttcagtcagg cggcattgct cgcccgcacg ctgctgatgc
     2161 gcaatcggga gttttttccc tttggcgcga cgatcagcac caagggcgcg gtgggtctgt
     2221 ccaccccggt ccccgccgcg gcgacgccca cggtgcacga cgtgatcgac gcgatcaaga
     2281 ccgagcttcg gcacgacaag gccggcaagc gcgcgtacgc gatcgtcgcc ctcgcccgga
     2341 cggcggacgg cgacgaggcc gtgcgcatcg aactcgagca caaagagggc atcgcgctgg
     2401 tcatcggcat gcccgtcctc cggggtgaat ccggaacggt gacgttcggg gacatggccg
     2461 cccatcccgg acaacggctc atctggccgc cgaaggcgcc cgcgggcgca aagggccccg
     2521 caacgaaggc cccggcgaag gctcccacga gggaccccgc gaagtcctcg gggctccctg
     2581 ccgccgcggc agcttcggcg gcccccaaga cggcatcgaa gcgtgcgcgc accgtggcgc
     2641 ccaaggcgaa gcccgctcga aagccccgcg gcacggccat cgcgacggcc taggccgcgc
     2701 ggactccccg ccgcaacccc cgcgtccgcc cctctatcct tgggctcgca cgcgcggccc
     2761 catcgaacac ccacgcccgg ggtgtatccg gtcggcgaat ccgcagactc ggcgcacccg
     2821 tgacgccgcc cggttcgttc cagccacgac gtgcgtcaag tgagttagga ttacctcacc
     2881 cgacatagcg tcggaaccct gtcaaaccaa ggaggacccg tgattgcggg ccgcagtagt
     2941 cgcattgccg tggtatcgct cgtcgccagc cttacccttc tcctcgcggg ctgcggcccg
     3001 aaggactcca ccccgacatc gtcgggcgca ggccccacat cctcc
//
LOCUS       DASVKP010000073         8344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_599254, whole genome shotgun sequence.
ACCESSION   DASVKP010000073 DASVKP010000000
VERSION     DASVKP010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599254"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1220)
                     /locus_tag="VF362_01520"
     CDS             complement(9..1220)
                     /locus_tag="VF362_01520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7589640.1"
                     /translation="MTRDDIPRPTVARSLASSVYLPTFFFEIGIGAIYPILALSAVHL
                     GASAAVASAAVGAYAMGRIVGSTFGGVFAARWGSPKAALAGMAWTVGAALVCAASGAL
                     AAFIVGALLVGMGHALVHVSRQSQVVEVVTFHYRARALTTLAGIWRIANFIGPLVGAI
                     AIDRYGLRGAFVFAAAAMASGGVALAATGSWRDHAHAPATRHVGPLRVARKNRRILST
                     LGLAVGLTGAVRAARLAALPLWAAHLGLADGTVSVIFAVSAAVDMMLFLPAGYVMDRR
                     DRRWTALPSTLLLTLGILALPLTSDVGTLTLAAIVIGVGNGWGSGLLMTLGSDVSPAE
                     GRHVFLGLWMMLQDVGGLAGPAIISVGAIASLPVGIVAVGAIGVGATGLLARWIPPWR
                     LSDGARAAALD"
     gene            complement(1225..2148)
                     /locus_tag="VF362_01525"
     CDS             complement(1225..2148)
                     /locus_tag="VF362_01525"
                     /inference="COORDINATES: protein motif:HMM:NF022674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HEX7589641.1"
                     /translation="MADAVLLDAVGIARDAAVDASGDPAMVGAHLGVHDVVDGVATHL
                     FACELPGYRGWVWEVAVADAPHATEATICESHLIPADDALLAPPWVPYEERVQPGDLE
                     PGMTLPFIAEDPRLVAGFSVTEDDEADAIAIWELGLGRERVLGPTGRDDAAERWHAGS
                     HGPTAPAAIAATAQCATCAFLVPLAGSMRGLFGACANEWSASDGEVVSLDHGCGAHSQ
                     TDLEKQGARWPANDPIIDTQDIDHLDLLAEDPPEAEATESPMADAGAEHDIETGQEPE
                     AEHDTDAEQDTDAEQPSAEAVAEPLADDTAE"
     gene            complement(2148..2531)
                     /locus_tag="VF362_01530"
     CDS             complement(2148..2531)
                     /locus_tag="VF362_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX7589642.1"
                     /translation="MPTGKVKWFDVDKGFGFIAADDGGEAFLHASVLPADAQAPKPGT
                     RVEYSVADGRRGPSAMAVTILEPAPSMVQAQRKPTDDMTVIIEDLIKMLDGVSNGLRR
                     GRYPDGAKGKQVSAVLRAVADQLEQ"
     gene            2807..3094
                     /locus_tag="VF362_01535"
     CDS             2807..3094
                     /locus_tag="VF362_01535"
                     /inference="COORDINATES: protein motif:HMM:NF022280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEX7589643.1"
                     /translation="MLYHVDAAEVSSAAGRAAASAETIRGEVASLIAHLESLQGTWQG
                     GAAQAFTGMLTQWRSAQAQLELALDGMSTGLSQAASQYDAAEQSAARLFAR"
     gene            3181..4551
                     /locus_tag="VF362_01540"
     CDS             3181..4551
                     /locus_tag="VF362_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7589644.1"
                     /translation="MGQRGVTEPVAGMHCSACEARVNAEFAALPGIQSVRANARRGEV
                     TYVASARFDHAKAERALAAHGYVLGARPYWLSRQARVWRDVAIGLVGVAIVATLARFG
                     VFSFAQGWVGALDGATLALPLLLGVAAGFSSCLATVGGLVLAVSASSAEGQRVGSVRA
                     SLRPHLVFNAGRVLGFGALGALTGALGHALIIGPVGLAVAMIVAGVAMVAVSMQMTVI
                     SPRLAGLAPRLPRWARVTDADGATGGRSPAGRDLRAAALGVASFFVPCGFTQAVQLFA
                     LSTGDPWRGGLVLAAFALGTTPALLLLGSAPALAQGRWRETFVRIAGVAVMAFAFVNV
                     AAAVSTLGLSISPKEPLPTATTSNVTMTDGVQVATLATDGAGYVPVDTVVYAGIPVRW
                     EITGNGLGCSSAIHAPELGVPPDTLIGYGKTEEFTFTLRKPGRVTYTCVMGMYSGTMT
                     AIAPPP"
     gene            complement(4617..6245)
                     /gene="groL"
                     /locus_tag="VF362_01545"
     CDS             complement(4617..6245)
                     /gene="groL"
                     /locus_tag="VF362_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115935.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX7589645.1"
                     /translation="MAKLIAFNEEARRGMERGMNALADAVKVTLGPKGRNVVLEKKWG
                     APTITNDGVSIAKEIELEDPLEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREG
                     LRNVAAGANPIALKRGIEKAVEAVTAELLKAAKEVETKEQIAATAGISAGDPVIGDLI
                     AEAMDKVGKEGVITVEESSGLGLELELTEGMRFDKGFLSAYFVTDPERQEAVLEDAYI
                     LLIESKISTIKDLLPLLEKVMQAGKPLAIVAEDVEGEALATLVVNKIRGTFRAVAVKA
                     PGFGDRRKAMLQDMAILTGGQVISESVGLSLENATLDLLGTARKVVVTKDETTIIEGG
                     GTPAQIEGRVAQIRKEIENSDSDYDKEKLQERLAKLAGGVAVIKAGAATEVELKERKH
                     RIEDAVRNAKAAVEEGIVAGGGVALIQAGEKALKNLKLEGDEATGARIVELSLSAPLR
                     QIAINAGLEGGVVVEKVRTLPSGHGLNAATGVYEDLMAAGVNDPVKVTRSALQNAASI
                     AALFLTTEAVVADKPEKEHPMSGGGGGDMGGMGF"
     gene            complement(6454..7056)
                     /locus_tag="VF362_01550"
     CDS             complement(6454..7056)
                     /locus_tag="VF362_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX7589646.1"
                     /translation="MSRNPQDPAPVAAHRLAPRGWTRIFTFLLVFALFGGIAYGGIWY
                     LDHNGKSWKDWIPFLSSGEPSPSPSASASATPGVTASPSPSPSVAKNYDLTVRVTIYN
                     ASGNPALGSDIAALLAADGRFKQIDTLPWSGALPPGNVVRFENPDLSDTANLVSQILK
                     IQTVASGPTSGPAIAVILVSDPRAQPKVTPSPGASPSASP"
     gene            complement(7066..7362)
                     /locus_tag="VF362_01555"
     CDS             complement(7066..7362)
                     /locus_tag="VF362_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HEX7589647.1"
                     /translation="MSEQARAPQQGANPGLSDRDTAILAFERAWWRYGGAKESSIRAE
                     FSMSPTEYYQVLNALLDDESALAHDPMLVKRLRRMRATRQRGRGTRRLEFPAAR"
     gene            7468..8148
                     /locus_tag="VF362_01560"
     CDS             7468..8148
                     /locus_tag="VF362_01560"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX7589648.1"
                     /translation="MSNAWRAQVAPDWVPVLDEVVPTLGSLGDFLREEIAAGRGYLPA
                     SADILRAFRTPLADVRVLIVVQDPYPTPGDPMVLSFSVPAGRPMPRSLANIARELRDD
                     TGETLASGDLTPWQEQGVMLLNRVLTVRPGSPGSHRGRGWEEVTERAIRALAGRGGPL
                     VAILWGRDARGLRPLLGDIPAVESPHPSPLSASSGFFGSRPFSRANALLAEQGATQIS
                     WGAPEASV"
     gene            complement(8145..>8344)
                     /locus_tag="VF362_01565"
     CDS             complement(8145..>8344)
                     /locus_tag="VF362_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589649.1"
                     /translation="GGAVAVSFSDVKGDLFSTKPGTVLAGLSFILPMVQNGGYTPSEV
                     VASTIILTIDGITTIPFTWTP"
BASE COUNT         1193 a         2920 c         2899 g         1332 t
ORIGIN      
        1 ttttccaact agtcgagcgc cgcggcccta gccccatcgc tcagcctcca cggcgggatc
       61 catcgcgcca ggagccctgt cgcgccgacg ccgatcgccc cgaccgcgac aatgccgacc
      121 ggcagcgacg cgatcgcgcc gaccgagatg atggcggggc ccgccagccc tcccacatcc
      181 tgcagcatca tccagagccc gaggaacacg tgccttccct ccgcgggcga gacgtcgctt
      241 ccgagcgtca tgaggagccc cgagccccag ccgttgccca ctccgatcac gatcgcggcg
      301 agtgtgaggg ttccgacgtc cgaggtcagg ggtagcgcca gtatccccag ggtgagaagc
      361 agcgtcgaag ggagcgccgt ccaacgccgg tcgcgccggt ccatgacgta ccccgcgggg
      421 aggaacagca tcatgtcgac cgcagcggag acggcgaaga tcaccgagac cgttccgtca
      481 gcaaggccaa ggtgcgccgc ccagagcgga agcgccgcga gccgtgccgc gcgcaccgca
      541 ccggtgaggc ccacggcgag gccgagggtc gagaggatcc ggcggttctt ccgcgcgacc
      601 cgtagcggac caacgtgccg cgttgccggg gcgtgcgcgt ggtccctcca cgaccccgtc
      661 gccgcgagcg ctacgcctcc cgaggccatc gccgccgccg cgaagacgaa cgccccgcgg
      721 agtccgtacc ggtctatggc gatagcgccc acgagcgggc cgatgaagtt cgcgatgcgc
      781 cagatccccg cgagcgtcgt gagtgcccgc gcgcggtagt ggaaggtcac cacctcgacg
      841 acttgggatt ggcgggagac gtgcacgagc gcgtgcccca tgccgacgag cagggcgccg
      901 acgatgaatg ccgcgagcgc gccgctcgcc gcacacacca gggcggcgcc aacggtccac
      961 gccatccccg caagcgccgc cttgggcgag ccccaccgcg cagcgaatac gccgccgaaa
     1021 gtgctaccga cgatgcgccc catcgcgtac gcgcccaccg cagcggaggc gacggccgcc
     1081 gaggcgccca agtgcaccgc ggacagcgcg aggatcgggt agatcgcgcc tatgccgatc
     1141 tcgaagaaga aggtggggag atagaccgac gaggcgaggc ttctcgcgac cgtgggccgc
     1201 ggaatgtcgt cccgcgtcac gcggctactc ggcggtgtcg tccgccagcg gctcggcgac
     1261 cgcttccgcg gacggttgct cggcgtccgt gtcctgctcg gcgtccgtgt cgtgctcggc
     1321 ttccggttcc tgcccggttt cgatgtcgtg ctcggcgccc gcatccgcca tcggggactc
     1381 cgtggcctcc gcctcgggag gatcctccgc caggagatcg aggtggtcga tgtcctgggt
     1441 gtcgatgatc ggatcgttgg ccggccaacg cgcgccctgc ttctcgaggt cggtctgcga
     1501 gtgggccccg cagccatgat cgaggctcac gacctcgccg tcggaggccg accattcgtt
     1561 cgcacatgcg ccgaacaggc cgcgcatgga tccggcgagg ggaacgagga aggcgcaggt
     1621 ggcgcactgc gccgtggcgg cgatggcggc gggcgccgtc gggccgtgag agcccgcgtg
     1681 ccaccgctcg gcggcgtcgt cgcggcccgt gggtcccagg acccgctccc gcccaagtcc
     1741 caactcccag atcgcgatcg cgtccgcctc gtcgtcctcc gtcacggaga agcccgccac
     1801 gagccgcgga tcctccgcga tgaacggcag ggtcatcccc ggctcaaggt ctccgggctg
     1861 gacccgctcc tcgtacggca cccacggcgg ggccaagagc gcatcgtccg ccgggatcag
     1921 gtgggactcg cagatggtcg cctcggtcgc gtgcggggcg tcggcaacgg cgacctccca
     1981 cacccatccg cggtagccag gcaactcgca cgcgaagagg tgcgtcgcca cgccgtccac
     2041 gacgtcatgc acgccaagat gcgccccgac catggcaggg tcccccgagg cgtcgacagc
     2101 ggcgtcgcgc gcgatgccga ccgcgtcgag aagaacggcg tcggccatca ctgctccaac
     2161 tgatcggcca cggcgcggag aaccgcggag acctgcttgc ccttggcccc gtccgggtag
     2221 cgcccgcgcc ggaggccatt cgagaccccg tcgagcattt tgatcaggtc ctcaatgatg
     2281 acggtcatgt cgtccgtggg cttgcgctgg gcctgcacca tggacggcgc gggctcgagg
     2341 atcgtgacgg ccatggccga ggggccgcgg cggccgtccg cgacggagta ctcgacgcgg
     2401 gtgccgggct tgggcgcctg ggcgtcggcg ggcaggacgg aggcgtggag gaaggcctct
     2461 ccgccatcat cggccgcgat gaagccgaag cccttgtcca cgtcgaacca cttcaccttg
     2521 ccggtcggca cgtcgtcctc gcttggtccc cagggcgcgg tggtccgcgc caatcgcatc
     2581 agtctatccg gaccttggcg cgcgaggcat tcggcggccg caacgggcgg ggcgcctcgc
     2641 ccgcgcgccc gtgggtggat gcccgcggtc gcggcgcggt cgtgccggaa cggtgagctg
     2701 atgtccccag gcgctccgga cccgcgcccc taccccgact tgacgttccc gccgccgtgc
     2761 ccgtcgtctc cctcctaggt tcgggtcacc aacccaagga ggagtcatgc tgtaccacgt
     2821 agacgctgcg gaggtgtcga gcgccgcagg ccgcgccgcc gcatcggccg agaccattcg
     2881 cggcgaggtc gcgagcctca tcgcccacct ggagagcctc cagggcacct ggcagggcgg
     2941 agccgcccag gccttcacgg gaatgctgac gcagtggcgt tcggcgcagg cccaactcga
     3001 gctcgcgctc gatggcatga gcacggggct ctcgcaggcg gcgagccagt acgacgcggc
     3061 cgagcagtct gcggctcgcc tctttgctcg ctgacaacgg cgcgctgacg acggccgcac
     3121 gccatctggg ggacctttgc cccgagcgcg gatcggttag gagcgcctta ctcttggctc
     3181 atgggtcaaa ggggcgtcac ggagccggtc gcgggcatgc actgctccgc gtgcgaagcg
     3241 cgcgtcaacg ccgagttcgc cgcgctcccg ggaattcagt ccgtgcgggc gaacgcgcgt
     3301 cgaggcgagg tcacgtacgt cgcgtccgcg cgcttcgacc atgcgaaggc cgagcgcgcc
     3361 ctcgccgccc acgggtacgt gcttggcgcg cgcccgtact ggctcagccg ccaggcccgg
     3421 gtgtggcggg atgtcgcgat cggcctggtg ggcgtcgcca tcgtcgccac gctcgcgcgg
     3481 ttcggcgtct tctcctttgc ccagggctgg gtgggggccc ttgacggcgc gaccctcgcg
     3541 ctcccgcttc tcctcggcgt tgccgcggga ttcagttcgt gtctcgccac ggtcgggggc
     3601 ctcgtcctcg ccgtctcggc gtcgtcggcc gagggccagc gagttggttc ggtgcgcgcc
     3661 tccctgcgcc cccacctcgt cttcaacgcc gggcgcgtcc tcgggttcgg agccctcggg
     3721 gcgctgacgg gcgcgctggg gcacgcgctc atcatcgggc ctgtcggcct cgccgtcgcg
     3781 atgattgtcg ccggcgtcgc gatggtcgcc gtgagcatgc agatgaccgt gatctcgcca
     3841 cgcctcgccg ggctcgcgcc gcgattgccg cggtgggccc gcgtgaccga cgccgacggc
     3901 gcgacgggcg ggcggtcacc agctggccgc gatcttcgcg cggccgcgct cggcgtcgca
     3961 tccttcttcg ttccgtgcgg cttcacccag gcggtccagc tcttcgcgct ctcgacgggc
     4021 gatccgtggc gcgggggtct cgtcctggcc gcctttgcgc tcgggacgac cccggcgctc
     4081 ctgctcctcg gtagcgcacc cgcactggct caaggacgtt ggcgcgagac attcgtgagg
     4141 atcgcgggcg tcgccgtaat ggcgttcgcg ttcgtcaacg tggccgcggc ggtgtcgacg
     4201 ctcgggcttt ccatctcgcc gaaggaaccc cttccgaccg ccaccacctc aaatgtcacc
     4261 atgacggacg gggtccaggt cgcgaccctc gcgaccgacg gggcgggata cgtgcccgtc
     4321 gatacggtcg tgtacgcggg aattcccgtg cgctgggaga tcacgggaaa cggccttggt
     4381 tgctcgtcgg cgatccacgc ccccgaactc ggcgtcccgc cggacacgct catcggctac
     4441 gggaagaccg aggagttcac cttcaccctc cgcaaacccg gcagggtgac ctacacgtgc
     4501 gtgatgggaa tgtactcggg gacgatgacg gctatcgccc cgcctccctg acacccaggg
     4561 cggacggaaa cagcgaggcc cgggggcgtg cccccgggcc tcgctgacgc gccgggctag
     4621 aagcccatac cgcccatgtc tccgccgccg cccccggaca tcgggtgctc cttctcgggc
     4681 ttgtccgcga caaccgcttc ggtcgtcagg aacagggccg cgatggaggc tgcattctgc
     4741 agcgccgatc gggtgacctt gacggggtca ttcactccgg cggccatgag gtcttcgtag
     4801 acccccgtcg ccgcgttcag gccatggccc gagggaaggg tgcgaacctt ctcgaccaca
     4861 acgccgcctt cgaggccggc gttgatcgcg atctggcgaa gcggagcgga gagcgaaagc
     4921 tccacgatcc gtgcgccggt cgcctcgtcg ccctcgagct tgaggttctt gagcgccttc
     4981 tcgcccgcct ggatgagcgc cacgccgcca ccggcgacga tgccctcttc gacggcggcc
     5041 ttcgcgttgc gaacggcgtc ctcgatgcgg tgcttgcgct ccttgagctc aacctctgtg
     5101 gccgcgccag ccttgatgac ggcgacgccg ccagcgagct tggcgagacg ctcctggagc
     5161 ttctccttgt cgtagtccga gtcggagttc tcgatctcct tgcggatctg agcgacgcga
     5221 ccctcgatct gggccggcgt tccgccgccc tcgatgatgg tcgtctcgtc ctttgtcacg
     5281 acgaccttgc gggcggtgcc cagaaggtcg agcgtggcgt tctcgagcga gaggcccaca
     5341 gactcggaga tgacctggcc accggtgagg atcgccatgt cctggagcat cgccttgcgg
     5401 cggtcgccga agccgggcgc cttgacggcg acggccctga atgtcccacg gatcttgttg
     5461 accaccaggg tcgcgagggc ctcgccctcg acgtcctcgg ccacgatcgc gagcggcttg
     5521 cccgcctgca tgaccttctc cagcaggggc aggaggtcct tgatcgtcga gatcttcgac
     5581 tcgatcagca ggatgtaggc gtcctcgagg acggcctcct ggcgctcggg gtcggtgacg
     5641 aagtacgcgg acaggaagcc cttgtcgaag cgcatgccct cggtgagttc gagctcaagc
     5701 ccaaggccgc tcgactcctc gacggtgatg acgccttcct tgccgacctt gtccatcgcc
     5761 tcggcgatga ggtcgccaat gacgggatcg cccgcggaga ttccggccgt ggcagcaatc
     5821 tgctccttgg tctcgacctc cttggccgcc ttgaggagct cggcggtgac ggcctcgacg
     5881 gccttctcga tgcctcgctt gagcgcgatc gggttggcgc cagcggcgac gttgcgaagg
     5941 ccctcgcgga ccagtgcctg agcgaggacg gtcgcggtgg tggttccgtc tcccgccacg
     6001 tcgtcggtct tcttggcgac ctccttgacg agctcggcgc cgatcttctc aaggggatcc
     6061 tcgagctcga tctccttggc gatcgacaca ccatcgttgg tgatggtggg cgcgccccac
     6121 ttcttctcta gtacgacgtt gcggcccttc gggccgaggg tgaccttgac ggcatcggcg
     6181 agggcattca tgccccgttc catgccgcga cgggcctcct cattgaaagc aatgagcttt
     6241 gccatgggga ctcttcctcc acgatcagtg ctgggccctc accgcccgcg acggccgacg
     6301 aacccacgac cgttcacgtc acggtcgcag acacgcctcg gcgaggtgcc tcggttgtta
     6361 gcactctcat gccgagagtg ctaatgcgat tctggcactc tcgccccccg agtgcaaact
     6421 cgcccgggga aggagggcgt cgtcccgcgg cccctagggg gacgcgctgg ggctcgcacc
     6481 cgggctcggc gtcacctttg gctgggctcg cgggtcgctg acgagaatca ccgcgatcgc
     6541 ggggccggac gtcggccctg acgccacggt ctggatcttg aggatctggc tcacgaggtt
     6601 cgccgtatcg ctcaggtcgg ggttctcgaa cctcacgaca tttcccggcg gaagcgcccc
     6661 gctccacggg agcgtatcga tttgcttgaa tcggccgtcc gcggcgagga gcgcggcgat
     6721 gtcggagccg agcgcggggt tgccggaggc gttgtagatc gtcacgcgca cggtcaggtc
     6781 gtagttcttg gcgaccgagg ggctcgggct tggcgaggcc gtcacgcccg gcgttgccga
     6841 ggcggacgcg gagggcgagg gcgagggttc gcccgaactg aggaacggga tccagtcctt
     6901 ccagctcttg ccgttgtggt cgaggtacca gatgccgccg tacgcgattc ctccgaaaag
     6961 ggcaaacacg aggaggaagg tgaagatgcg agtccacccg cgcggtgcga ggcggtgggc
     7021 ggcgacgggc gcgggatcct gggggttgcg ggacacggga cgaccttacc ttgccgccgg
     7081 gaactccagg cgacgggtgc cacgcccccg ctggcgggtg gccctcatcc ttcggaggcg
     7141 cttgacgagc atggggtcgt gggcgagggc ggactcgtcg tcaaggagcg cgttgaggac
     7201 ctggtagtac tcggtcggac tcatcgagaa ctcggcgcgg atggaggatt ccttggcgcc
     7261 gccgtagcgc caccacgccc gctcgaacgc gaggattgcc gtgtcacgat cggaaaggcc
     7321 ggggttcgcg ccctgctggg gtgcgcgcgc ctgctcgctc atctcgcctc ctcggagccc
     7381 ggggactgca tcccccaagc gcaactgtac cgccggaatc acatcgatgt cattcgacat
     7441 ctcgggcgga gtggataccc tgacgccatg tccaacgcgt ggcgcgccca agtggctccc
     7501 gactgggtcc ccgtgctcga cgaggtcgtc ccgacgctcg ggagcctggg tgacttcctg
     7561 cgggaggaga tcgctgcggg ccgcggatac cttcccgcga gcgcggacat tctgcgggcc
     7621 ttccgcacgc cgctggcgga cgtccgcgtc ctgatcgtag tccaggaccc ctaccccacc
     7681 ccgggcgacc cgatggtcct cagcttctcc gtgccggctg gcaggccgat gccgcgctca
     7741 ctcgccaata tcgcgcgcga actccgagat gacaccgggg agacgctcgc gtccggcgac
     7801 ctgacgccgt ggcaggagca gggggtgatg cttctcaaca gggtcctgac ggtgcggccg
     7861 gggagcccgg gatcccacag ggggcgcggg tgggaagagg tgaccgagcg ggcgattcgc
     7921 gcgctcgcgg ggcgcggcgg gccgctcgtg gcgatcctct ggggccgcga cgctcggggg
     7981 ctaaggccac tgctcggtga catccctgcg gtggagagcc cgcatccgag cccgctcagc
     8041 gcatcctcgg ggttcttcgg ctcgcggccg ttctcgcgcg cgaacgccct gctcgcggag
     8101 cagggcgcta cgcagatctc ctggggtgcg cccgaagcct cggtctaggg cgtccaggtg
     8161 aagggaatcg tagtgattcc gtcgatcgtg agaatgatgg tcgacgcgac cacttcggaa
     8221 ggcgtgtacc ccccgttctg gaccatgggc aggatgaacg acagaccggc caggaccgta
     8281 cccggtttcg tagaaaagag gtccccctta acgtcggaga aactgaccgc gaccgcccct
     8341 ccca
//
LOCUS       DASVKP010000074         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049 k141_21389,
            whole genome shotgun sequence.
ACCESSION   DASVKP010000074 DASVKP010000000
VERSION     DASVKP010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21389"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="VF362_01570"
     CDS             <1..1334
                     /locus_tag="VF362_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7589650.1"
                     /translation="LLNLGLLAFRSDDAITTDVLCFQLLVVVVALVGGIFPAVFAAVA
                     AALLLDFYFVTPWHTITIAEPLHLLALVIFVIVAMLVSIVVDLAARRLRAASRSSAES
                     ATLALVAGSVLRGQDALDALVGQLREAFGMTSVILREGDAAIYNSTDWSVAHEGDVET
                     TFPLGEAASLCVRGRALPASDRRVLTAFLSQIGVALRERQLSREAEGMRPIAEADRLR
                     TALLAAVGHDLRRPLAAATAAVTSLRSTEVELSAGDRGALLETADESLASLAHLVTNL
                     LDVSRMQAGVLGVSISTVMLDDVVLLALDELALSPGDVQLELGQGIRAARADPVLLQR
                     VVVNLLANALRFSPAGQAPIIITSEFLARVELRVVDSGPGIPAERRDQIFVPFQRLGD
                     TDNATGIGLGLALSKGFVEAMSGSLDVEDTPGGGLTMVVSLPADAQTGVTP"
     gene            1331..>1713
                     /locus_tag="VF362_01575"
     CDS             1331..>1713
                     /locus_tag="VF362_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7589651.1"
                     /translation="MRILIADDDPQILRALRITLTARGYDIVTASDGKEALRLAADKH
                     PDLIVLDLGMPGLTGTEVIEGVRGWSRIPILVVSGRSGSRDKVDALDAGADDYVTKPF
                     AADELLARIRALSRRTPAATDEPVVA"
BASE COUNT          231 a          612 c          582 g          288 t
ORIGIN      
        1 cgctcctgaa cctcgggcta ctggcgtttc gcagcgacga cgcgattacc accgacgtcc
       61 tgtgcttcca gctcctcgtt gtggtggtcg ccctcgtcgg cggcatcttt cccgcggtgt
      121 tcgccgcggt ggccgcggcc ctcctgctcg acttctactt cgtcaccccc tggcacacga
      181 tcacgatcgc cgagcccctc cacctcctcg cgctcgtcat cttcgtcatc gtcgcgatgc
      241 tcgtgagcat cgtcgtcgac ctcgcggcgc ggcgactccg tgcggcgagc cgttcctcgg
      301 ccgaatccgc gaccctcgct ctcgtcgccg ggagtgtgtt gcgcgggcaa gacgccctcg
      361 acgcgctcgt cgggcaactg cgcgaggcct tcgggatgac aagcgtcatc ctgcgcgagg
      421 gcgacgcggc gatctacaac tcgacggact ggagcgtcgc ccatgagggc gacgtcgaga
      481 cgacgttccc ccttggagag gcagcgagcc tctgcgtgcg cggacgcgcg cttcccgcct
      541 ccgaccgacg ggtgctaacg gccttcctgt cgcagatcgg cgtcgcgttg cgcgagcggc
      601 aactctcgcg cgaggccgaa ggcatgcggc ccattgcgga ggcggaccgg cttcgcaccg
      661 cgctcctcgc ggccgtgggc catgacctgc gccgtcccct cgcggcggcg acggcagccg
      721 tgacgagcct ccggtccacc gaggtggagc tgtccgcggg cgaccgcgga gcgctcctcg
      781 agactgcgga cgagagcctc gcctccctcg cccaccttgt gacgaacctc ctcgatgtca
      841 gccgcatgca ggcgggcgtg ctgggggtct cgatctccac ggtgatgctc gacgatgtcg
      901 tgctgctcgc cctcgatgaa ctggcccttt cgcccgggga cgtccaactc gaactggggc
      961 agggcatccg tgccgcgcgc gcggacccgg tgctcctcca gcgtgtggtg gtcaacctgc
     1021 tcgccaacgc gctgcggttc tctccggcgg gacaagcgcc catcatcatc acgagcgagt
     1081 tcctggcgcg ggttgagttg cgcgtggtcg acagcggccc gggaatccct gccgagcggc
     1141 gcgaccagat tttcgtgccg ttccagcgcc tgggcgacac ggacaacgcg accggcatcg
     1201 gcctcggcct cgcgctatcg aagggcttcg tcgaggcgat gagtggctcg cttgacgtgg
     1261 aggacacccc gggcggcggc ctcaccatgg tggtctcgct tcccgccgac gcgcagactg
     1321 gagtgacgcc gtgaggatac tgatcgccga cgacgacccg cagatcctgc gggccttgcg
     1381 catcacgctg acggcgcggg gatacgacat cgtcacggcg tccgacggca aggaggcttt
     1441 acggctcgcg gccgacaagc acccggatct gatcgtgctc gacctcggga tgccgggcct
     1501 gacggggacc gaggtgatcg aaggggtgcg gggatggtcg cgcatcccga ttctggtcgt
     1561 ctcgggtcgc agcggatcgc gggacaaggt ggacgccctc gacgccgggg cggacgacta
     1621 cgtgacgaag ccctttgcgg cggacgagtt gctcgcccgc attcgcgccc tatcccgccg
     1681 cactcccgcc gcgaccgacg agccggtggt cgc
//
LOCUS       DASVKP010000075         4302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_353717, whole genome shotgun sequence.
ACCESSION   DASVKP010000075 DASVKP010000000
VERSION     DASVKP010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353717"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VF362_01580"
     CDS             complement(<1..986)
                     /locus_tag="VF362_01580"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX7589652.1"
                     /translation="MMPRVGAAEARRIFLAAQGLARRRPTGRVGERQFREYLGHQGVL
                     QLDSVNVFARAHYLPLFSRYGAYDTAALDRYLWNSGETFEHWGHEASVMPRELMGHLH
                     FRMSASGTWKEQTRGYLERERPGLIAAVEAAVRQSGPLSAADLAHLTPDAVRQPGSWW
                     NSGDVKTALEYLFITGRVAVASRPHFQRRYDDPVRVWGRQAREGALHADEARQGLFDH
                     SLPAAGIGTPKDFTDHFRLPANQAARFAGSAVERGLAAWVEVEGWGEPALLATGAADP
                     GRATGAALLSPFDPACRYRDRLLRLFGMHYRIEIYTPEAKREYGYYTLPFLLG"
     gene            1059..1131
                     /locus_tag="VF362_01585"
     tRNA            1059..1131
                     /locus_tag="VF362_01585"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1092..1094,aa:Thr,seq:ggt)
     gene            1172..1245
                     /locus_tag="VF362_01590"
     tRNA            1172..1245
                     /locus_tag="VF362_01590"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1206..1208,aa:Met,seq:cat)
     gene            1280..1456
                     /gene="rpmG"
                     /locus_tag="VF362_01595"
     CDS             1280..1456
                     /gene="rpmG"
                     /locus_tag="VF362_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003829846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX7589653.1"
                     /translation="MDVASKATDIRPKITLACTECKERNYITKKNRRNTPDRLELAKF
                     CPRCGKQTPHRETR"
     gene            1462..1908
                     /locus_tag="VF362_01600"
     CDS             1462..1908
                     /locus_tag="VF362_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7589654.1"
                     /translation="MSVNPDAVGRSYGPGEPYEVTRVKIREFADAVKAPSDAHRSVEA
                     AKAAGFDDLVAPTTFAVVIAQRAEFPAIMDPEVGVDFSKVVHTDERFTHHRPLVAGDV
                     ISAVVHVDSIVQRAGISMVTTRAELVDAAGALVATVVSTLAVREDA"
     gene            1908..2381
                     /locus_tag="VF362_01605"
     CDS             1908..2381
                     /locus_tag="VF362_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX7589655.1"
                     /translation="MTASLPGATRPAAAIPAPARPVFAGLAVGDVVATQDVAITRDTL
                     VRYAGASGDFNPIHYNDAFATSVGLPGVIAHGMFTMGAAASVVESWAGPGNVVDFQAR
                     FTRPIAVPNPGEAVATVTCAIGALDADARTARVDVTVEFEGQKVLGKAQAVVACA"
     gene            complement(2473..3531)
                     /locus_tag="VF362_01610"
     CDS             complement(2473..3531)
                     /locus_tag="VF362_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7589656.1"
                     /translation="MGAIRRPTMADVAEKAGVSLSTVSLTFSGAGPISDETKLRVEKA
                     AADLGYGGPSPLGKSLRSGRSHIVGVVLAHNLGRAFRDPNALRVMDGVVSSLGDMGLG
                     VLLVPAPTGKEGERSLLETAPMDAAILMRVRGHDDPGIEIAKKRGIPVAVMEGPAPSG
                     SGLVTIDDERATVDLIAHLRGLGHERIATVTLPLGDDAETQVVGPERFRAAVWAPAHN
                     RLAAFGAAGIEPCVVVEARASMVEEGIAAGHLALSHPSKPTAIVCQSDLLAAGVVLAA
                     RELDIGVPQDVSITGFDGIDVPWLAPHELTTVIQDAPRMGQLLAAEVRTLLDGETPEP
                     IQLPVVLRIGTTTARARA"
     gene            3696..>4302
                     /locus_tag="VF362_01615"
     CDS             3696..>4302
                     /locus_tag="VF362_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589657.1"
                     /translation="MMSATRHQPTSKPDSARIAQGRLGVMGLFVSLGMMVSTFLSRLP
                     SIRTELDVTKGQLASLLIVGAIGAFIALMLTGWAVAKFGTRRLHVWSSVGYAAAFVGV
                     GFSSHFGLAMAFAGFQLLTNFAFAFTNVAMNAEAALTERLTGRKIMPHFHAGFSIGMA
                     LGLGLGWLASKLGVAPMVHFAVVAVVLLVIRLILIPIAVTDG"
BASE COUNT          634 a         1525 c         1475 g          668 t
ORIGIN      
        1 ccgaggagga acggcaacgt gtagtacccg tactcccgct tggcctcggg cgtgtagatc
       61 tcgatgcggt agtgcatgcc gaacaagcgc aggaggcggt cgcggtagcg gcacgcgggg
      121 tcgaacgggc tgagaagcgc ggcgcccgtc gcgcgaccgg gatccgcggc gccggtggcg
      181 aggagggcgg gctcgcccca cccttccacc tcgacccacg cggctagccc gcgctccacc
      241 gcggagccag cgaacctcgc ggcctggttc gccggcagcc ggaagtgatc cgtgaagtcc
      301 ttgggcgtgc cgattcccgc cgcggggagc gagtggtcga agagtccctg cctggcctcg
      361 tcggcgtgga gcgcgccctc gcgcgcctgg cgcccccaga cgcggacggg atcgtcgtag
      421 cgccgctgga agtgtggcct cgacgcgacg gcgacccgcc cggtgatgaa caggtactcg
      481 agcgcggtct tcacgtcgcc gctgttccac cacgagcccg gctgacgcac ggcatccggg
      541 gtgaggtgag caagatcggc cgcggagagc gggccggatt ggcggacggc ggcctcgacg
      601 gccgcgatca ggccgggacg ctcgcgttcg aggtacccgc gcgtctgctc cttccacgtc
      661 ccggaggcgc tcatgcggaa gtggaggtgc cccatgagtt cgcggggcat caccgacgcc
      721 tcgtgacccc agtgctcgaa cgtctcccct gagttccaca ggtagcggtc gagcgctgcg
      781 gtgtcgtacg ccccgtaccg cgagaacagc ggcagatagt gggccctggc gaacacgttg
      841 accgagtcca actggaggac gccctggtgg ccgaggtact cgcggaactg ccgttcgccc
      901 accctgcccg tcggccgtcg ccgcgcaagg ccctgcgccg cgaggaagat tcggcgggcc
      961 tcggccgcgc ccacgcgggg catcatgcgc ccaccctagc cgggacgccc gacgccggtt
     1021 cgcgtggggc gcatgcgccc ggtatgctgt tccggcttgc cccgatagct cagtcggtag
     1081 agcgtttcca tggtaaggaa aaggtccagg gttcgattcc ctgtcggggc tcggatgcga
     1141 gcggttaccc tggggtgacg gcacgttgcc tggcggggta gctcaggtgg ttagagcaca
     1201 cgactcataa tcgtggggtc gcgggttcga gtcccgccct cgctacagac tcgaaacaag
     1261 cgcgcatagc gcgaaggaga tggacgtggc aagcaaggca acggatattc gccccaagat
     1321 cactctggcg tgcacggagt gcaaggagcg aaactacatc accaagaaga accgtcgcaa
     1381 cacgccggac cgcctcgagc tggcgaagtt ctgcccgcgc tgcgggaagc agactcctca
     1441 ccgcgagacg cgctaggtag catgtcggtc aaccccgacg cggtgggccg gtcctacggc
     1501 ccgggggaac cgtacgaggt gacgcgggtg aagatccgcg aattcgccga cgcagtgaag
     1561 gctccctccg acgcgcatcg ttccgtcgag gcggcgaagg ccgcagggtt cgatgacctc
     1621 gtggccccca cgaccttcgc cgtggtgata gcccagcggg ccgagttccc cgcgatcatg
     1681 gaccccgagg tcggcgtgga cttctccaag gtcgtgcaca cggacgaacg cttcacccac
     1741 caccgcccgc tcgtcgcggg agacgtgatc tccgccgtcg tgcacgtcga ttcgatcgtt
     1801 cagcgcgcgg gcatctccat ggtgaccacc cgcgccgaac tcgtggatgc cgcgggagcg
     1861 ctcgtcgcca cggtggtctc cacgctcgcc gtgagggagg acgcctgatg accgcgtctc
     1921 tccccggcgc aacacgtcct gccgctgcca tcccagcccc cgctcgcccc gtcttcgcgg
     1981 gcctcgccgt cggcgacgtc gtcgccacgc aggacgttgc gatcacgcgg gacacgctcg
     2041 tccgctacgc cggagcgtcg ggcgacttca acccgatcca ctacaacgac gccttcgcga
     2101 cgtcggttgg gctccccggc gtcatcgccc acggcatgtt caccatgggt gcggcggcct
     2161 cggtcgtcga gtcgtgggcc ggaccgggga acgtcgtgga cttccaggcg cggttcactc
     2221 gcccgatcgc cgtgcccaat cccggcgagg cggtggccac ggtcacctgc gcgatcggcg
     2281 cgctcgacgc cgacgcgagg accgcgcgcg tcgacgtgac tgtggaattc gagggccaga
     2341 aggtgctcgg caaggctcag gccgtcgtcg cctgcgcgta gcccgcgccg cccgcgccgc
     2401 cccgcggcgc gccccgctcc gcccctctcg acccgggaaa ccggcggcgc cccttcggcg
     2461 ttccccggtg cttcaggccc gcgcgcgggc ggtcgtggtg ccaatgcgga gcacgactgg
     2521 caactggatc ggctccggcg tctccccgtc gagcaacgtg cgcacctccg cggcgaggag
     2581 ttgccccatg cggggcgcgt cctggatcac ggtcgtgagc tcgtgtgggg cgagccacgg
     2641 cacgtcgatg ccgtcgaatc ccgtgatgct cacgtcctgc gggaccccga tgtcgagttc
     2701 cctcgccgcg agcacgacgc cggccgcgag caggtccgac tggcagacga tggccgtggg
     2761 cttgctcgga tgggagagcg cgaggtggcc cgccgcgatt ccctcctcga ccatcgacgc
     2821 cctggcctcg acaacgacgc acggctcgat cccggcggcc ccgaacgccg caagccggtt
     2881 gtgcgcgggg gcccacaccg cggcccggaa gcgctcgggt ccgacgacct gcgtctccgc
     2941 gtcgtccccg agcggcaggg tgacggtggc gatgcgctcg tgccccaggc cgcgcaggtg
     3001 ggcgatcaga tccacggtcg cgcgctcgtc gtcgatggtg acgaggccgg atccggacgg
     3061 ggccgggccc tccatcaccg caaccggaat cccgcgcttc ttggcgatct cgatcccggg
     3121 atcatcgtgc ccccgcactc tcataaggat ggcggcatcc atcggcgcgg tctccaggag
     3181 cgagcgctcc ccctccttgc ccgtcggcgc gggaaccagg agcaccccga ggcccatgtc
     3241 gccgagtgaa ctgacgacgc catccatgac gcggagggcg ttcgggtcgc ggaaggccct
     3301 ccccagattg tgggcgagca cgacgccgac gatgtggctt cgccccgatc gcagcgactt
     3361 gccgaggggg ctgggtccgc cgtagccaag gtcggcagcg gccttctcca cccggagctt
     3421 ggtctcgtcg gaaatggggc cggcgccgga gaaggtcagg gagaccgtgg agagggagac
     3481 gcccgccttc tcggcgacgt ccgccatggt cgggcggcgg attgcaccca cgatgacacc
     3541 tccgcgattt gactgtcgcc cagcgtaccg gcacaatcga caatggtgat cgaaacgatt
     3601 cgacttggtc gtccgatccc gcactcctac gccaacgacg gcggcccgcg cgcgcctgtc
     3661 ggcccgcgcg aggctatgcc ccaaaggacc ccttcatgat gtcggcgacg cgccaccagc
     3721 ccacgagcaa gcccgactcc gcgcgcatcg cgcaggggcg gctgggggtg atggggctct
     3781 tcgtcagcct gggcatgatg gtctcgacgt tcctgtctag gcttccatcg atccgcaccg
     3841 aactggacgt gaccaagggg caactcgcct ccctcctgat cgtcggcgcc atcggcgcgt
     3901 tcatcgccct gatgctcacc gggtgggccg tcgcgaagtt cgggactcgg cgccttcatg
     3961 tgtggtcgtc cgtcggctac gcggccgcct tcgtgggggt ggggttctcc tcccacttcg
     4021 gcctcgcgat ggccttcgcg ggcttccaac tcctgacgaa cttcgccttc gccttcacca
     4081 acgtcgcgat gaatgccgag gcggcgttga ccgaacggct cacgggccgc aagatcatgc
     4141 cgcacttcca cgccggcttc tccatcggca tggcccttgg cctgggcctt gggtggctcg
     4201 cctcgaaact cggcgtcgcg ccgatggttc acttcgccgt ggtggcggtc gtccttctcg
     4261 tgattcgcct catcctgatc ccgatcgccg tcacagacgg ga
//
LOCUS       DASVKP010000076         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1176837, whole genome shotgun sequence.
ACCESSION   DASVKP010000076 DASVKP010000000
VERSION     DASVKP010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176837"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="VF362_01620"
     CDS             complement(<1..1192)
                     /locus_tag="VF362_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HEX7589658.1"
                     /translation="MSGGESGAPGAPAPEGFIPLSVPEFHGNEWLYVKECLDTGWVSS
                     VGSYVERFEADMASATGTRFAVATMNGTSALHTALLVAGVRPDDEVLVSTLTFIAPAN
                     AVRYVGARPVFIDAEPATGQMDPSRVAEFLDRGCVTRGGEVFDRETGRRVKAIIPVHI
                     LGHPVDMDPVLELARRYGLAVVEDATESLGAEYKGRRVGQLGDIACFSFNGNKIITTG
                     GGGMIVTNDEAWARKARYLTTQAKDDPVEFVHDEIGYNYRLTNVLAAIGVAQLERLPE
                     YVAAKRRIAARYADALGAVPGLSCLGEAPWARATFWMSVVRVDREAFGADSRALLRAL
                     EARRIQTRPLWQPIHLSRPHVGSTRLGGDVAERLHRDCLTLPCSVGLTEEQQSRVIDG
                     VVSFV"
     gene            complement(1189..>1595)
                     /locus_tag="VF362_01625"
     CDS             complement(1189..>1595)
                     /locus_tag="VF362_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7589659.1"
                     /translation="NEFTMEMMTKGKLVVFGEQFWRPYIHVRDAARALALVLGAPETA
                     VRGRVFNVGATKENYQKKQLVELIKPQAPGAVVEYVHRDEDPRDYRVSFERVRSELGF
                     ETIRTVPDGIAEVARLVRSGVLTDFADPRYRN"
BASE COUNT          253 a          554 c          535 g          253 t
ORIGIN      
        1 gcacgaaaga gacgaccccg tcgatgactc gcgactgctg ctcctccgtc aggccaaccg
       61 agcacggcag ggtgagacag tcgcggtgga gtcgctcggc cacgtcaccc cccagccggg
      121 tcgagccaac gtgcggcctc gagaggtgga tcggctgcca gaggggccgc gtctggatgc
      181 ggcgtgcctc gagggcgcgg agaagcgcgc gggagtcggc gccgaacgcc tcccgatcca
      241 cccgcacgac cgacatccag aaggtggccc gggcccacgg cgcctctccc aagcacgaca
      301 gccccgggac cgcccccagc gcatcggcgt accgggcggc gattcgccgc ttcgcggcca
      361 cgtactccgg cagccgctcg agctgggcca cgccgatcgc ggccaggacg ttcgtcaacc
      421 ggtaattgta gccgatctcg tcgtggacga actccacggg atcgtccttg gcctgggtcg
      481 tcaggtagcg ggccttccgc gcccaagcct cgtcgttggt cacgatcatc ccgccgccgc
      541 cggtcgtgat gatcttgttc ccgttgaagc tgaagcaggc gatgtctccg agctgcccca
      601 cgcgacgccc tttgtactcg gcaccgaggg actcggtcgc gtcctcgacg acggcgaggc
      661 cgtaccgccg cgccagctcg aggaccgggt ccatgtcgac cggatggccg agaatgtgaa
      721 cggggatgat ggccttgacg cgccgccccg tttcgcggtc gaagacctcg ccgccgcgtg
      781 tgacgcaccc gcggtccaga aactcggcga cgcgcgaggg gtccatctgt cctgtcgcgg
      841 gctccgcgtc gatgaagacg ggccgggcgc ccacgtatcg gacggcattg gccggcgcga
      901 tgaaggtgag ggtcgagacg aggacctcgt cgtcgggccg cacccccgct acgagaagtg
      961 ccgtgtggag cgcggacgta ccgttcatcg tcgcgacagc gaagcgcgtc cccgtcgcgc
     1021 tcgccatgtc tgcctcgaat cgttcgacgt aggagccgac cgatgagacc cagcccgtgt
     1081 ccagacactc cttgacgtac aaccactcgt tcccgtggaa ctcgggaacg gagagcggga
     1141 tgaagccctc gggtgccggg gcgccggggg ccccgctctc gccgccgctc aattgcggta
     1201 cctcggatcg gcgaagtcgg tgaggacgcc ggacctgacg agccgcgcga cttcggcgat
     1261 cccgtcggga acagtgcgga tggtctcgaa gcccagctcg cttcgcacgc gctcgaacga
     1321 gacgcggtaa tcgcgcgggt cctcgtcccg gtgaacgtac tcgacgaccg ccccgggcgc
     1381 ctgtggtttt atgagctcga cgagctgctt cttctggtag ttctccttgg ttgctccgac
     1441 gttgaagacg cggcccctca ccgcagtttc cggtgccccc aggacgaggg ccagggcccg
     1501 ggcggcgtct cggacgtgga tgtacggacg ccagaactgt tccccgaaga caacgagctt
     1561 ccccttcgtc atcatctcca tcgtgaactc gttga
//
LOCUS       DASVKP010000077         7976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1093944, whole genome shotgun sequence.
ACCESSION   DASVKP010000077 DASVKP010000000
VERSION     DASVKP010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 7976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7976
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093944"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VF362_01630"
     CDS             complement(<1..342)
                     /locus_tag="VF362_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX7589660.1"
                     /translation="MKAPKDPSSRAGRLRGKLAFGLGDRLRSAFSRGGADVDWEDLEE
                     TLIGADLGVAAAQEVLDAVRASLGKSKDADPHESLRNALLGALGHADRALALGPPGSS
                     VGASIVVVGVNG"
     gene            complement(358..612)
                     /locus_tag="VF362_01635"
     CDS             complement(358..612)
                     /locus_tag="VF362_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589661.1"
                     /translation="MLGVLRVPITIRLAVEFSIALAAIICVIGICAQGRLKHVLLWLL
                     GAVPRKRPSFSTRVPRAEHHWALIGSLSADAREEPQSPPT"
     gene            738..2369
                     /locus_tag="VF362_01640"
     CDS             738..2369
                     /locus_tag="VF362_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEX7589662.1"
                     /translation="MATSTPGGYRKCPNGHAALSGDRFCATCGAAVAVRRAKPAAEAK
                     PATPAKPSAGGKVNVRAKPAAEAKPAAEAKPAADAKPSEAKFAVARKPSEAGGAATPA
                     APTRAEPAPGFASEPDARLWNPRRRGGSFGATTWIAAAMLVATVVVGVVVAVKLLGGS
                     SRDAEGPGGRTGFATACDGIAQEEITIGQDAITRNSLLCFRVTEHSEITIDATPNGTD
                     ADLQLTVGLGTGKVITEDDNTDGLKPVVVFEAQPGTYILSVTQHGGGSPGTITVHSSA
                     VALPEVGLSALPTLDECANLSGPTLTDGGTEPRAAGEPFTCLSLTAGAFTKIGAVAYD
                     PAKTDLTLAVYAFDGAGVAQFVRSVDDTFATDPELNLDLAAGNYLIEVGAYDAGKMGA
                     YSLSVDTTGTFFRTGAVSSGLATLRPADCDSLPAVTVGAPLTIGLPLATGTAGEPLAC
                     LTLDSPRRLVIMAATQAGQDLTLEIVGFDSTGSPVRYVWADEDVFGEDPNSQDPRTDL
                     LLPAGTYVLAVNEYWGAEVAHDFVVTVTPGAGPNG"
     gene            complement(2366..5905)
                     /gene="smc"
                     /locus_tag="VF362_01645"
     CDS             complement(2366..5905)
                     /gene="smc"
                     /locus_tag="VF362_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX7589663.1"
                     /translation="MHLKTLTLRGFKSFASVTTLDFEPGITCVVGPNGSGKSNIVDAL
                     SWVMGEQGAKTLRGGTMEDVIFAGTPGRPPLGRAEVSLTIDNHDGVLPIDYSEVTISR
                     TLFRSGGSEYGINGNPCRLLDIQELLSDAGLGREMHVIVSQGHLDDILRGTPEDRRHF
                     IEEAAGILKHRKRKEKALRKLDAMQGNLTRIQDLTGEIRRQLGPLGKQAEVAREAQKI
                     QAAARDARARLLADDLSQVSAHLQQEQADESALLARRATVEKALAEARARMVELERAL
                     AEATPALTKANDVYYRLSAIRERLRNLATLAEERLRMIGTHVDAFAPNDIVDLEVQVE
                     RAKVAQAELDAEVAAARGALEAAEAARQEAEAVALASERSLANLLRSAADRREGVARL
                     AGDVAARRSRVEASEDEIGRLQQALAEAEARGRDATHEFSALESTVASVEVGEEDLDA
                     AHEKASEAWETAKAALATSRVRLTEAILERDTWAAKAEALELSLARKDGAGALLASGA
                     RGVRGTVASLIDVSDQAEDAVAAALGPLAEALAVDSVEDAVDAIRWLRDADEGRARFV
                     VGDRSAVATNPDVALPRGAAWASDLVNGPAGIVDTVRAALSGVAVVDDLAAARALVAA
                     HAGVTAVTRRGDVLSVRRASGGSGDAPSILHVQAAFDEACERRDAASRVVETTTFEIR
                     AAEQHESAAHEAHDGALERLHESDAQMAAVAEHLGQLGAASRAAREEADRLRERLEAA
                     QDRIRAEQEELGGLSQRLGAAQAEPEQTEADTLVAHAERDAHTEAAVLARAGETEARL
                     ALRTSEERARALSARAESLQLAAEAERDARARVEESAQKRSRQALAAREVLAGARFAL
                     ERIDASVQGADDARTTVEAERLERAVQLAEVRRGVDEYGAEYARLTDDAHKDEVARAE
                     QRIRLEQFQQRAIDELGLDPQVLVDEYGPHLAVPPEGESDLASPYVREDQERRLRRAE
                     RGLMQLGRVNPLALEEFAALEERHAFLSEQLADIKKSRAHLMEIVKEIDDRVETVFAE
                     AFADTAKAFEEVFPRLFPGGEGRLVLTDPTDMLMTGIDVEARPAGKKVKRLSLLSGGE
                     RSLAAIGMLIAIFKARPSPFYGMDEVEAALDEVNLGRLLDILHELQSDSQLIIITHQK
                     RTMEIADALYGATMRGDGVTTIISQRLRESELA"
     gene            complement(5957..6376)
                     /gene="ndk"
                     /locus_tag="VF362_01650"
     CDS             complement(5957..6376)
                     /gene="ndk"
                     /locus_tag="VF362_01650"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX7589664.1"
                     /translation="MTERTLVLAKPDAVERGLVGEIVKRIESKGYRLVAVDLLTPDRA
                     TLERHYAEHEGKPFFPPLVEFMSSGQVFAMVVEGDRVIAGFRSLAGATDPTVAAPGTI
                     RGDFAREWNAPVMRNLVHGSDSEVSAAREIAIWFPSL"
     gene            complement(6408..6899)
                     /locus_tag="VF362_01655"
     CDS             complement(6408..6899)
                     /locus_tag="VF362_01655"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - obsolete nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX7589665.1"
                     /translation="MIERTLLLIKPDAIERRLIGEVIRRAEVKGYDVIALEMVRPGRG
                     ILERHYAQHRGKVFFQPLIDFLLSGPLIACVLQGDRVIEGLRSLVGTSDPTTAAPGSI
                     RGDLARDWDTPVYHTLVHGLDSPLAAHAEIELWFPNLPPMRLRRPLGSSSMPPPPPPP
                     MMG"
     gene            6955..7452
                     /locus_tag="VF362_01660"
     CDS             6955..7452
                     /locus_tag="VF362_01660"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX7589666.1"
                     /translation="MDDFDTYPDFAVAGILFYDIGPILRDPDLFSLACQDLTPAAGTP
                     VDMIAGVDARGFIFAPVVAQRLHVGLTMVRKEGKMSGDLLTATASIEYGAASLTLNPK
                     GLAGKDILVVDDVLATGGTARAVAQMLEEAGATSVSFSFLPELTALGGRRALEEYSVS
                     SAVVV"
     gene            complement(7449..>7976)
                     /locus_tag="VF362_01665"
     CDS             complement(7449..>7976)
                     /locus_tag="VF362_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589667.1"
                     /translation="RGAPRRANPARGRSRMTGTLWKLGGCLAAGLAALTMVLWGLERS
                     ALVAAAVRVGGESASIPTRAYVLVFAGLVLLSLAVYCALTTWGGFLKANPETRQLPVW
                     LLLAVIVVAGGAGLLGLAAHWGYIRSLDVVPMAVNQGYIAYQVLTSAAILASMVVMGV
                     RWAPGYRPRVLGPRP"
BASE COUNT         1172 a         2883 c         2753 g         1168 t
ORIGIN      
        1 gccgttgacc ccgacgacga cgatggatgc acccaccgat gacccaggcg gtccgagcgc
       61 cagggcacga tccgcgtgcc ccagcgcgcc gaggagggcg ttcctgaggg actcatgagg
      121 gtcggcgtcc ttggacttcc cgagcgatgc ccgcacggcg tcgagcacct cctgcgccgc
      181 cgcgacgccg aggtcggcac cgatcagcgt ctcttccagg tcctcccagt cgacgtcggc
      241 gccacccctg gagaaggcgg atcgcaggcg atcccccagg ccgaacgcca acttcccgcg
      301 cagacggccc gctcgcgacg atgggtcctt gggagccttc acccgcctag tgtcgcacta
      361 cgtcggcgga gactgcggct cctcacgcgc gtcggcggag aggcttccga tgagcgccca
      421 gtgatgctcg gcgcgcggaa ccctggtaga gaacgagggc cgcttgcgcg ggaccgcccc
      481 caagagccac aacaacacgt gcttcagccg gccctgcgcg cagatgccga tcacgcagat
      541 gatcgccgcc agcgcgatgg aaaactccac cgcaagccga atagtaatgg gcacgcgcaa
      601 cacccccagc attgatgcag tctatccagg acaatcttgc tccattttcg ccatcttcgc
      661 tcggtgagcg ggcgatcatc gagcgcacga atcctccgca actgtggcaa agggcgcgcc
      721 gcggatagag tcctcccatg gcaacgtcga cgccgggcgg gtatcggaag tgccccaacg
      781 ggcacgccgc gctgtccggc gacaggttct gcgcgacgtg cggcgccgcc gtcgcggtgc
      841 gtcgagcgaa gcccgcggcg gaggcgaagc ccgccacccc ggcgaagccg agcgccggag
      901 ggaaggtcaa cgtccgggcg aagcccgccg ccgaggcgaa gcccgccgcc gaggcgaagc
      961 ccgcggcgga cgcaaagccc tccgaggcga agttcgccgt cgcacggaag ccctccgagg
     1021 cggggggagc ggcgaccccc gccgccccga cccgggcgga gcctgccccc ggcttcgctt
     1081 ccgaaccgga cgcccgcctg tggaatccgc gacgccgggg cggatccttt ggggcgacca
     1141 cctggatcgc ggccgcgatg ctcgtcgcca ccgtcgtcgt cggggtggtc gtcgccgtga
     1201 agctcctcgg cgggagttcg agagacgccg agggtcccgg aggccgcacg ggtttcgcga
     1261 cggcgtgcga cggcatcgcc caggaggaga tcaccatcgg gcaggacgcg atcacgcgga
     1321 actctctcct gtgcttccgg gtcacggagc attcggagat cacgatcgat gccactccca
     1381 acggaaccga cgccgacctc cagttgacgg tgggcctggg gacggggaag gtgatcaccg
     1441 aggatgacaa caccgacggc cttaagcccg tggtggtgtt cgaggcccaa cccggtacct
     1501 acatcctcag cgtcacgcag cacgggggag gaagcccggg cacgatcacc gtgcactcct
     1561 ccgccgtcgc cctccccgag gtcggcctaa gcgcgctccc caccctcgac gagtgcgcga
     1621 acctcagcgg gccgacgctc actgacgggg gaaccgagcc tcgggcggcg ggagagccct
     1681 tcacctgcct gagcctcacc gcgggcgcgt tcaccaagat cggagcggtg gcgtacgacc
     1741 cggccaagac ggacctcacg ctggcggtct acgccttcga cggggcgggg gttgcgcagt
     1801 tcgtgcgcag cgtcgacgac accttcgcga ccgacccgga actcaacctc gacctcgccg
     1861 ccgggaacta cctcatcgag gtgggcgcct acgacgccgg caagatgggc gcgtactccc
     1921 tgtccgtcga cacgacggga acattcttca ggacgggcgc cgtgtcgagc gggctcgcca
     1981 ccctgcgccc ggcggactgc gactcgctcc ccgccgtcac ggtgggcgcg ccgctcacga
     2041 tcggcctgcc cctcgcgacc ggaacagcgg gcgagccgct cgcgtgcctt accctcgact
     2101 ctccccggcg cctggtcatc atggccgcga cgcaggcggg ccaggatctg acactggaga
     2161 tcgtcggctt cgactccacg ggttcccccg tccgctacgt gtgggcggat gaggacgtct
     2221 tcggcgagga cccgaacagc caggaccccc gcaccgacct gctgcttccc gccggaacat
     2281 acgtgcttgc cgtcaacgag tactgggggg cggaggtcgc ccacgacttc gtggtgaccg
     2341 tgacgccggg ggccggtccg aacggctagg cgagttcgct ttccctgagc cgctggctga
     2401 tgatagtcgt cacgccgtcg ccacgcatgg tcgcgccgta gagggcatcc gcgatctcca
     2461 tcgtccgctt ctggtgggtg atgatgatga gttgagagtc cgactgcaac tcgtggagga
     2521 tgtcgagaag cctgccaagg ttgacctcgt cgagggcggc ctccacctcg tccatcccgt
     2581 agaaggggct cgggcgcgcc ttgaagatgg cgatcagcat gccgatcgcc gcgagcgatc
     2641 gctcgccccc gctgagcaac gacagtcgct tgaccttctt gcccgcgggg cgcgcctcga
     2701 catcgatccc cgtcatgagc atgtcggtcg gatccgtcag gacgaggcgg ccctcgccgc
     2761 cggggaacag gcgcgggaac acctcctcga acgccttggc cgtgtcggcg aaggcctccg
     2821 cgaagaccgt ctccacgcgg tcatcgatct ccttcacgat ctccatgagg tgggcgcgcg
     2881 acttcttgat gtccgcgagt tgttcgctga gaaacgcgtg gcgctcctcg agggccgcga
     2941 actcctcgag cgcgagcggg ttgacgcggc cgagttgcat cagtccccgc tcagcgcggc
     3001 gcaggcggcg ctcctggtcc tcccgcacgt aaggcgaggc gaggtcggac tcgccctcgg
     3061 gcggaaccgc cagatgcggg ccgtattcgt cgacgagcac ctggggatcg agtccgagtt
     3121 catcgatcgc gcgctgctgg aactgctcga gccggatacg ctgctccgcg cgcgcgacct
     3181 cgtccttgtg ggcgtcgtcc gtgagcctgg cgtactcggc gccgtactcg tcgacgccgc
     3241 gcctgacctc ggcgagctgc acggcgcgct cgaggcgctc ggcctcgacg gtcgttcgcg
     3301 cgtcgtcggc gccctggacg ctcgcgtcga tccgctcaag ggcgaagcgg gccccggcca
     3361 gcacctcccg cgccgccagc gcctggcggg accgcttctg ggcggactcc tcgaccctgg
     3421 cccgcgcgtc gcgctcggcc tccgccgcga gctggaggct ctcggcgcgg gccgagagcg
     3481 ccctcgcccg ctcctccgag gtgcggaggg caaggcgggc ctccgtttcc cccgccctcg
     3541 ctagcaccgc cgcctccgtg tgggcatcgc gctcggcgtg ggcgactagc gtgtccgcct
     3601 cggtctgctc cggctcggcc tgggccgcgc cgaggcgttg gctcagtccc ccgagctcct
     3661 cctgctcggc gcggatgcgg tcctgggcgg cctctagccg ctcgcgaagt cggtccgcct
     3721 cctcccgcgc tgcgcgggac gccgcgccaa gttgcccgag gtgctcggcc acggccgcca
     3781 tctgcgcatc cgactcgtgg agtcgctcga gcgctccatc gtgggcctcg tgcgcggccg
     3841 actcgtgctg ctcggcggcc cggatctcga aggtcgtcgt ctcgaccacg cgcgacgccg
     3901 cgtcgcgacg ctcgcacgcc tcatcgaacg ctgcctgaac gtggaggatg ctcggcgcgt
     3961 cgccgctccc gcccgaggcc cgccgcacgc tcaggacgtc gcccctgcgc gtcacggcgg
     4021 tcacccccgc gtgggcagct accagggccc tggccgcagc aaggtcgtcc acgacggcga
     4081 ccccgctaag agcggcccgc acggtgtcga cgattcccgc gggcccgttg acgaggtccg
     4141 acgcccacgc ggctccgcgc ggcagggcaa cgtcggggtt ggttgccacg gcggagcggt
     4201 cgccgacgac gaagcgcgcc ctgccctcgt ccgcgtcgcg cagccaacgg atggcgtcga
     4261 ccgcatcctc gacgctgtcc acggcgagcg cttcggcgag cgggccgagg gcagccgcca
     4321 cggcgtcctc cgcctggtcc gacacgtcga tgagcgaggc gacggtcccc cgaacccccc
     4381 gggccccgga cgcgagtagc gcgccggcgc cgtccttgcg cgcgaggcta agttcgagcg
     4441 cctcggcctt cgccgcccag gtgtcgcgct cgaggatggc ctcggtgagg cgcacacgcg
     4501 aggtcgcgag cgcggccttc gctgtctccc acgcctccga ggccttctcg tgggcggcgt
     4561 caaggtcctc ctcgccgacc tcgacggatg cgacggtcga ctcaagcgcg gagaactcgt
     4621 gcgtcgcatc gcggcctcgc gcctccgcct cggcgagcgc ctgctggagg cggccgatct
     4681 cgtcctcgga cgcctcgacg cgcgaacgcc gcgccgcgac gtccccggcg agtcgcgcca
     4741 cgccctcgcg tcggtcggcg gccgagcgga gaaggttcgc gagcgaacgc tccgacgcga
     4801 gggctacggc ctccgcctcc tggcgcgcgg cctctgcggc ctcaagcgct ccgcgggccg
     4861 cggccacctc ggcgtcgagc tcggcctgcg cgaccttggc gcgctccacc tggacctcga
     4921 ggtccacgat gtcgttcggg gcgaacgcat ccacgtgcgt cccgatcatc ctgaggcgct
     4981 cctcggcgag cgtcgcgagg ttcctcagcc gctcccggat ggcgctcagc cggtagtaga
     5041 cgtcattcgc cttggtgagc gctggggtcg cctccgccaa ggccctttcc aactccacca
     5101 tccgcgcgcg ggcctcggcg agcgccttct cgaccgtcgc gcgacgcgcg agcagcgccg
     5161 actcgtccgc ctgctcctgc tgaaggtgcg ccgagacctg ggagaggtcg tcggcgagga
     5221 gcctggcgcg cgcgtcccga gccgccgcct ggatcttctg ggcctcgcgc gcgacctccg
     5281 cctgcttgcc gagggggccg agctggcgcc ggatctcgcc cgtgaggtcc tggattcgcg
     5341 tgaggttccc ctgcatcgcg tcgagcttgc gtagcgcctt ctccttgcgc ttgcggtgct
     5401 tgaggatgcc cgccgcctct tcgatgaagt gccgccgatc ctcgggcgtg ccgcgaagga
     5461 tgtcatcgag gtggccctgc gagacgatga cgtgcatctc tcgcccaagg cccgcgtcgc
     5521 tcaggagttc ctggatgtcg agcagccgac aggggttgcc gttgatcccg tactccgagc
     5581 cgcccgagcg aaacagggtg cgcgagatcg tcacttcgga gtagtcgatg ggcagcacgc
     5641 cgtcgtggtt gtcgatcgtg agcgagacct cggcgcgacc gagcgggggg cggccggggg
     5701 tgcccgcgaa gatgacgtcc tccatggtgc cgccgcggag cgtcttggct ccctgctcgc
     5761 ccatcaccca ggacagcgcg tcgacgatgt tggacttgcc cgacccgttc ggcccgacga
     5821 cgcaggtgat gcccggctcg aaatcgagag tcgtcaccga cgcgaaggac ttgaagcccc
     5881 ggagcgtcag cgtcttgagg tgcacggagg caagcctagg cgatgggagg gaccctccgg
     5941 gagcggcccg gtggctctag agactcggga accagatggc gatctcgcgg gccgccgaga
     6001 cctcggagtc cgagccgtga acgaggttcc tcatcaccgg cgcattccat tcgcgcgcga
     6061 agtccccgcg gatcgtccca ggagccgcga ccgtgggatc ggtcgcgccc gcgagggaac
     6121 ggaaccccgc gatcacgcgg tccccttcga ccaccatggc gaacacctgt ccagaactca
     6181 tgaactccac caacggcggg aagaagggct tgccctcgtg ctccgcgtag tggcgctcga
     6241 gcgtggctcg gtcgggcgtc aggaggtcca ccgcaacgag gcggtagccc ttcgactcga
     6301 tccgcttgac gatctcgccc acgaggccgc gctcgacggc atcgggcttg gcaaggacta
     6361 gggtgcgctc ggtcatggca acgatgctac ccgcgcggac agcgccccta gcccatcatg
     6421 ggcggcggag ggggcggggg catggacgag gaacctagtg gccgccgtag gcgcatcggc
     6481 ggcagattgg ggaaccacaa ctcgatctcg gcgtgcgcgg cgaggggcga atctaatccg
     6541 tggacgaggg tgtgatagac cggcgtgtcc cagtcgcgtg cgagatcccc acgaatgctc
     6601 cccggcgccg ccgtggtggg gtcgctcgtg ccgacgagcg agcgcaggcc ctcgatcacc
     6661 ctgtccccct ggaggacgca tgcgatcaac gggccggaga ggaggaagtc gatgagcggc
     6721 tggaagaaca ccttgccccg gtgctgggcg tagtggcgct ccaggatccc tcggcccggc
     6781 cggaccatct ccagcgcgat cacgtcgtag cccttgacct ctgcgcggcg gatgacctcc
     6841 ccgatgaggc gtcgctcgat ggcgtcgggc ttgatgagca gaagcgtccg ttcgatcatg
     6901 atccgaacat gaccctcagg cgccattggc tggcaggatt gcaacgtcct gaccatcgac
     6961 gacttcgaca cgtaccccga cttcgccgtc gccgggatcc tgttctacga catcgggccc
     7021 atcctgcgcg accccgacct cttctcgctg gcgtgccagg acctgacgcc cgcggcggga
     7081 accccggtcg acatgatcgc gggcgtcgac gcgcgcgggt tcatcttcgc ccctgtggtc
     7141 gcgcaacgcc tccatgtggg cctgacgatg gtccgcaagg agggcaagat gtcgggcgat
     7201 ctcctcaccg cgacggcaag catcgagtat ggcgccgcga gcctcacgct caatcccaag
     7261 ggcctcgcgg gcaaggacat cctcgtggtg gacgatgtgc tcgcgacagg ggggaccgcg
     7321 cgcgccgtcg cccagatgct tgaggaagcg ggcgcgacca gcgtctcgtt ctcgttcctg
     7381 cccgaactca ccgcgctcgg ggggcgccgc gcgctggagg agtactcggt ctcgtccgcc
     7441 gtggtcgtct aggggcgagg gcccaggacg cgcgggcggt atccgggagc ccaccggacc
     7501 cccatgacga ccatggatgc gaggatggcg gcggacgtaa ggacctggta ggcgatgtag
     7561 ccctggttga cggccatcgg cacgacgtcg agcgagcgga tgtagcccca gtgcgccgcg
     7621 agtcccagga gccccgcgcc ccccgcgacg acgatgacgg cgaggaggag ccacaccggg
     7681 agttgccgag tctcgggatt ggccttcagg aagccgcccc aggtcgtgag cgcgcagtac
     7741 accgccaggc tcaggagtac gagaccggcg aacacgagca cataggcgcg cgtcgggatg
     7801 gacgccgact ctcccccgac gcgaacggcc gccgcgacga gcgccgagcg ctcgagtccc
     7861 cacagaacca tcgtgagcgc ggccaacccc gccgccaggc agcccccgag cttccacagc
     7921 gtgcccgtca tgcggctccg tccccgggct gggttcgcgc gtcgcggcgc gccccg
//
LOCUS       DASVKP010000078         7493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_354815, whole genome shotgun sequence.
ACCESSION   DASVKP010000078 DASVKP010000000
VERSION     DASVKP010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354815"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2414)
                     /locus_tag="VF362_01670"
     CDS             complement(<1..2414)
                     /locus_tag="VF362_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEX7589668.1"
                     /translation="MSIRRIGSRAALAAMMALAFGLAGGMIPAAADQGDSFDGDPNLI
                     RNPDFTIDYEGWSAYGVTGGVEDGMFCGEYFGPHRNEYDAGFGFNGMSFPAGDYFASF
                     DAKSEVGFIAKVQQSGGGYARLGTLNVPPSDTLQHYEMTFSSDVPFQYGEYHFHVGTP
                     VAGPHTFCVDNVVLKVHPKDYAVGGDFADGLGGWSVAGATAAVSDAGVCLAVPEGTSA
                     SDNVSLRLANVALPKFSYELRRDASGEGVPMRVLVTSHDDPSLVYADSTETLTSDTST
                     SSSYFLLPGKGDRKLEGDNVDVSFEFGGGAGGQVCLDNAQLLSGGQPPPYEPETGPRV
                     RVNQYGYETDGPKRATLVTDVTDPVAWRLVDAAGATAAEGTSVPFGGDASSRLVVQTI
                     DFSEVTAEGTYTLVADDDESYPFAIATDLYGPLRDDALHYFYLARSGIEIDAALAGDD
                     YARAAGHVSTAGGSDTNQGDYAVACQPAGDSEPIYGEPWTCDYTLDVVGGWYDAGDHG
                     KYVVNGGIAAYQLMSAYERTLREDGDARKELGDSVLDVPETGNGIPDILDEAKWELDF
                     MVSMMVPEGEQYAGMVHHKVHDFGWTSLPLMPADDGYVRYLHRPSTAATLNLAATAAQ
                     GARLFKKFDRSYADSLLTAAKTAWDAAIATPDLYAPAADGTAGGGSYDDKYVSDEFYW
                     AAAELYLTTRDSKYEDYLLASPLLTANVFTAGAFGWSETAALGQLDLATVNSDLPGRE
                     GIIASVVEGADALLAVQAEQGFGQALAADKFVWGSNSTVLNNQVVLGTAYDLTGDPKY
                     LSAVRG"
     gene            complement(2566..2638)
                     /locus_tag="VF362_01675"
     tRNA            complement(2566..2638)
                     /locus_tag="VF362_01675"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2602..2604),aa:Glu,seq:ctc)
     gene            complement(2741..2812)
                     /locus_tag="VF362_01680"
     tRNA            complement(2741..2812)
                     /locus_tag="VF362_01680"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2777..2779),aa:Gln,seq:ctg)
     gene            complement(2908..3666)
                     /locus_tag="VF362_01685"
     CDS             complement(2908..3666)
                     /locus_tag="VF362_01685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7589669.1"
                     /translation="MPPPPTDLLADVSGVLIDIDDTLVDNKSAIRHAISAIAGAYLPS
                     ETDIDEAAAFFRDDRHGHYRAFARGEIDSRTQRWMRAKDLHDHFGGTPLDDEAAAGPW
                     IDAFDAAYAAGWRIHDDSVAFLATLDALGLPYGLLSNARRAQQVAKLAAVGLSAVPLL
                     ACVDDFGYAKPDPRIFLEACTLLGTEPARTVYVGDEFDIDAAAAVRAGLVGVWCDRPG
                     AWERWGDADVEGSGALRVESLAGLASLLGPRSTP"
     gene            complement(3716..5200)
                     /gene="gltX"
                     /locus_tag="VF362_01690"
     CDS             complement(3716..5200)
                     /gene="gltX"
                     /locus_tag="VF362_01690"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX7589670.1"
                     /translation="MSGKVRVRFCPSPTGTPHVGLIRTALFNWAYARHTGGDFVFRIE
                     DTDAERDSQESYDQLLDALRWLGLDWDEGPEVGGPYGPYRQSERSAIYTDVAERLVLG
                     GYAYESFSTPEEIEARHLAAGRDPKLGYDGFDRALTDQQRDAYRAEGRDPVIRLRMPD
                     DDISFTDLIRGHVTFRAGSVPDFVIVRGNGQPLYTLVNPVDDALMKITHVLRGEDLLS
                     STPRQIALYRALEELGLADGVPTFAHLPMVTGEGNKKLSKRAPESNLFLHRERGFLPE
                     GLLNYLGLLGWSISGDRDLFTLSEMVAAFDVADVNPNPARFDLKKAEAINAEHMRLLP
                     LGEFVVRLVPYLHAAGVIGASHVRDLTPHEEATLALAAPLVQERMTLLGEAPGMLGFF
                     FVADSALVVEDDARATLPANAAAILDAAIGVIEGLDDFTAEAQQEALRAALVEGMGLK
                     PRDAFTPLRVAVTGRRVSPPLFESMEILGQDHSLARLRALRASL"
     gene            complement(5197..5985)
                     /locus_tag="VF362_01695"
     CDS             complement(5197..5985)
                     /locus_tag="VF362_01695"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010534875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7589671.1"
                     /translation="MRIARFTTGSEPRYAKVEGAPGEEELLVLTGDPLYVPGEPTGER
                     VPLSAEGLRLLAPIIPRSKAVCMGRNYAEHAKELGNVGKSGDLPLLFLKPNTAVVGPD
                     DPIVLPHYSEDVQHEAELAVVIGRLIKDVSPEGALAAIYGYTCANDVTARDAQKAENQ
                     WFRSKAFDTSLPLGPWIETDLDTGDLEIEARVNGERRQFGNTHDMIWDAAQIVAMVSE
                     VVTLLPGDVILTGTPAGVSTLRHGDRVEIEIEGIGVLSSPVVRR"
     gene            6033..6536
                     /locus_tag="VF362_01700"
     CDS             6033..6536
                     /locus_tag="VF362_01700"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEX7589672.1"
                     /translation="MPQPAATLLDELAARADLRLDTLVAMIVANGLEEASLGDAYLGR
                     RGADVLAHLHGWHGLFAGWCREDGRGDEPTLPATGYTWEDLRALNDEIYERYRAMPWT
                     RILEVTRESHATMIELLRTFPVGALADASRYPWANGPLIDLADECLGKHYDWGIERIE
                     EYVRAHE"
     gene            6559..7047
                     /gene="ybaK"
                     /locus_tag="VF362_01705"
     CDS             6559..7047
                     /gene="ybaK"
                     /locus_tag="VF362_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEX7589673.1"
                     /translation="MNTHASTPAIAALVRANVAHTLHPYEHDPGSDLGYGLEAAAAIG
                     VEPSRVFKTLCVEADGALAVAIVPVDAMLDLKSLASALGAKKAAMAEAVAAERATGYV
                     VGGISPIGQKRRLRTVLDSSALAFDAVYVSGGRRGLDVGLAPGDLVAVTGAITAPIAR
                     DS"
     gene            7044..>7493
                     /locus_tag="VF362_01710"
     CDS             7044..>7493
                     /locus_tag="VF362_01710"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX7589674.1"
                     /translation="MTPGDEVSSDAASPDAVAPYAARLAEVKARVRAAEAACGRAGAG
                     VRVLVATKTRDEATAEAVCAAGARLVGENRVQELVAKGPVFRAAGVETHLIGPLQRNK
                     ARDAVAWADCIETVDSLALTERISGLAFEAGRTLGIMVQVNVSGEGTK"
BASE COUNT         1156 a         2534 c         2674 g         1129 t
ORIGIN      
        1 ctcccgcgca cagctgagag gtacttcggg tctcccgtta ggtcgtacgc cgtccccaga
       61 accacctggt tgttgagcac cgtcgagttg gagccccaca cgaatttgtc ggcggcgagc
      121 gcctggccga agccctgctc cgcctgcacg gcgaggagcg cgtcggcccc ctcgacgacg
      181 gacgcgatga ttccctcacg cccgggaagg tcgctgttca cggtggcaag gtccaattgg
      241 ccgagcgcgg ccgtctcgct ccagccgaat gccccggcag tgaacacgtt cgcggtgagc
      301 agaggcgagg cgagcaggta gtcctcgtat tttgaatccc gggtcgtgag gtacaactcc
      361 gccgcggccc agtagaactc gtccgagacg tacttgtcgt cataagagcc gccgcccgcc
      421 gtgccgtccg cggcgggcgc gtacaggtct ggggtcgcga tggccgcgtc ccaagccgtc
      481 ttcgccgccg tgaggagcga gtccgcatag ctgcggtcga atttcttgaa gagccgcgcg
      541 ccctgggcgg ccgtcgcggc aaggttgagc gtcgcggcgg tcgaggggcg gtgcaggtag
      601 cgcacgtagc cgtcgtcggc tggcatgagc ggaaggctcg tccacccaaa gtcgtggacc
      661 ttgtggtgca ccatccccgc gtactgctcg ccctcgggca ccatcatcga gaccatgaag
      721 tcgagctccc acttggcctc atcgaggatg tcggggatgc cgttgccggt ctcgggcacg
      781 tcgagcaccg agtcgccaag ctctttgcgc gcgtcgccat cctcacggag cgtgcgctcg
      841 tacgccgaca tcagttgata ggccgcgatg ccgccgttga ccacgtactt gccgtggtcg
      901 ccggcgtcgt accagccacc gaccacgtcg agcgtgtagt cgcaggtcca tggctcgccg
      961 tagatcggct ccgagtcgcc cgcgggttgg caggcgacgg cgtagtcgcc ctggttcgtg
     1021 tcggagccgc ccgcggtact cacgtggccc gccgcgcggg catagtcgtc cccggcgagg
     1081 gcggcgtcaa tctcgatgcc cgagcgcgcc aggtagaagt agtggagggc gtcgtcgcgc
     1141 agcgggccgt agaggtcggt cgcgatcgca aacgggtaac tctcgtcgtc gtccgccaca
     1201 agcgtgtacg tgccctctgc ggtgacctcg gagaagtcga tggtctggac gacgaggcgg
     1261 gaagaggcgt caccaccgaa cgggacggac gtgccttccg cggccgtagc gcccgccgca
     1321 tccacgagcc gccacgcgac ggggtcggtc acgtcggtca cgagggtggc gcgtttcggt
     1381 ccgtcggtct cgtagccgta ctggttgacg cgcacgcggg gaccggtctc cggctcgtag
     1441 ggcggcggct ggcctcccga gagcaactgg gcgttgtcga ggcacacctg tccccccgcg
     1501 ccgccgccga actcgaacga cacgtccacg ttatcgccct cgagcttgcg gtcacccttg
     1561 cccggaagca agaagtacga ggatgacgtc gacgtgtccg aggtgagggt ttcggtgctg
     1621 tccgcgtaga ccagggacgg atcgtcgtgc gaggtcacga gcacgcgcat gggcacgccc
     1681 tcgcccgagg catctcggcg cagttcgtac gagaacttcg gcagcgccac gttcgccagg
     1741 cgcaggctca cgttgtccga ggcggaggtg ccctcgggca cggcgagaca cacgccggcg
     1801 tcgctcaccg ctgccgtggc acccgcgacg ctccagccgc cgaggccatc ggcgaagtcc
     1861 ccaccaacgg cgtagtcctt gggatgcacc ttgaggacga cgttgtcgac gcagaaggtg
     1921 tggggcccgg ccacgggtgt gccgacgtgg aagtggtact cgccgtactg gaacggcacg
     1981 tcggaggaga aggtcatctc gtagtgctgg agggtgtcgg agggcgggac gttgagcgtg
     2041 ccgaggcgcg cgtatccgcc gccgctctgc tgcaccttgg cgatgaagcc aacctccgac
     2101 ttggcgtcga acgacgcgaa gtagtcgccc gcggggaacg acatgccgtt gaagccgaag
     2161 cccgcgtcgt actcgttccg gtgcgggcca aagtactcgc cgcagaacat gccatcctcg
     2221 acgccaccgg tgacgccgta ggcgctccac ccttcgtagt cgatggtgaa gtccgggtta
     2281 cggatcagat tcgggtcgcc atcgaaggag tcgccttggt cggcggcggc ggggatcatc
     2341 ccaccggcca gcccgaacgc cagcgccatc attgccgcga gcgccgctcg agagccgatc
     2401 cgtcgaatgc tcaccgtgac ctccttgtca cgcgcgccgc atcgccattg accgtcgcgt
     2461 cttgagcgag aggctaccat gagagcgctc ccatagaaac gcctcgaagt gtggcgtcgt
     2521 agcgatttcg cggggacggc tgattgggga ggtgccgaag agaagtagcc ccgaccggat
     2581 tcgaaccggc gctaccgccg tgagagggcg gcgtgctagg ccgctacaca acggggcccc
     2641 tagccgtccc gagccgaggc ccgggaacgc gaattccaag ggtagcgcat gcgccttgcc
     2701 ctcgtgaaac ggcgaaggcc cgccgaagcg ggcctcgcag ctggggtaca aggattcgaa
     2761 cctcgactaa ctggaccaga accagtcgtg ctgccagtta caccataccc caagggtcct
     2821 tttgggaacc ggggcaccac tgtaccgggt cggcggtgaa gccccaaacg cgaagcctcg
     2881 cgcgcgcgaa tgcccccgtg ccttgtgtca gggagttgat cgcggcccga gcaggctagc
     2941 gagccccgcc agggactcga cgcgcagcgc gccggagcct tcgacgtccg cgtctcccca
     3001 gcgctcccac gcgccagggc gatcgcacca gacgccgacg aggcccgcgc gaaccgccgc
     3061 cgccgcgtcg atgtcgaact cgtcgcccac gtacacggtg cgggcgggct cggtccccag
     3121 gagcgtgcac gcctcaagga agatacgggg gtccggcttg gcatacccaa agtcgtcgac
     3181 gcacgccaag agcgggacag cggagagccc cacggccgcg agcttggcca cctgctgcgc
     3241 gcggcgcgca ttgctcaaga ggccgtacgg aaggccgagg gcgtcgagcg tggcgaggaa
     3301 ggcgaccgag tcgtcgtgga tcctccagcc cgccgcgtag gcggcgtcga aggcgtcgat
     3361 ccagggaccg gcggcggcct cgtcgtcgag gggcgtcccg ccgaagtgat cgtggaggtc
     3421 cttggcccgc atccagcgct gggtgcgcga gtcgatctcg cccctggcga acgcccggta
     3481 gtggccgtgg cgatcgtccc gaaagaacgc cgcggcctcg tcgatgtccg tctcgctcgg
     3541 caggtaggcg cccgcaatcg ccgagattgc gtgacggatc gcggacttgt tgtcgaccag
     3601 ggtgtcgtcg atgtcgatca acaccccgga gacgtccgcg agcaggtccg ttggaggcgg
     3661 cggcaccgag cctggcggca ccgagcctgg tggcaccgaa ttcaccgcgc gacgctcaca
     3721 gggaggcgcg cagcgcgcgc agtcgcgcga gtgagtggtc ctggccgagg atctccatgg
     3781 actcgaacag cggcggcgac acccggcgtc ccgtcacggc cacgcgaagc ggggtgaacg
     3841 cgtcacgcgg cttgagtccc atgccctcga ccagcgcggc gcgcaacgcc tcctgctgcg
     3901 cctcggcggt gaagtcatcg agcccctcga tcacgccgat cgccgcgtcg aggatcgccg
     3961 cggcgttcgc gggcagggtc gccctcgcgt cgtcctccac cacgagcgcg gagtcggcga
     4021 cgaagaagaa gccgagcatc cccggcgcct cgcccagcag cgtcatgcgc tcctgcacca
     4081 gcggcgcggc gagcgcgagc gtcgcctcct cgtggggcgt gaggtcgcgg acgtgggagg
     4141 cgccgatgac gccggccgca tgcaggtagg ggacgagccg aacgacgaac tccccgagcg
     4201 ggagcaggcg catgtgctca gcgttgatcg cctcggcctt cttgaggtcg aagcgggcgg
     4261 gattggggtt gacgtccgcg acgtcgaacg cggcgaccat ctcgctcaac gtgaagaggt
     4321 cgcgatcccc tgagatcgac cagccgagca ggccgaggta gttcaggagc ccctcgggga
     4381 ggaacccgcg ctcgcggtgg aggaagaggt tggactcggg ggcccgcttc gagagcttct
     4441 tgttgccctc ccccgtcacc atcggcaggt gcgcgaacgt cgggacgccg tctgcgagcc
     4501 cgagttcctc aagcgcccgg tagagcgcga tctgccgggg tgtcgagctg agcagatcct
     4561 cgccgcgcag cacgtgagtg atcttcatga gcgcgtcgtc cacgggattg acgagcgtgt
     4621 acagcggttg cccgttgccg cgcacgatga cgaagtccgg aaccgagccg gcgcggaagg
     4681 tgacgtggcc gcggatcaga tcggtgaacg agatgtcgtc gtccggcatg cgcaggcgga
     4741 tgacggggtc gcgcccctcg gcgcgatacg cgtccctctg ctggtccgtg agggcgcggt
     4801 cgaacccgtc gtagccaagc ttggggtcgc ggcccgccgc gaggtggcgc gcctcgatct
     4861 cctcgggagt cgagaacgat tcgtacgcgt agccgcccag gacgagcctc tcggcgacgt
     4921 cggtgtagat cgcgctccgt tcgctctggc ggtaagggcc ataggggccg cccacctccg
     4981 ggccctcgtc ccagtcgagg ccgagccagc gaagggcgtc cagcagttga tcgtaggact
     5041 cctggctgtc gcgctcggcg tccgtgtcct cgatccggaa gacgaagtcc ccacccgtgt
     5101 gccgcgcgta cgcccagttg aacagcgcgg tgcggatcag cccgacgtgg ggcgtgcccg
     5161 tgggcgacgg gcagaagcgg acgcggacct tgccgctcat cgccgcacca ccgggctcga
     5221 caggacgccg atgccctcga tctcgatctc gacgcgatcg ccgtgcctca gcgtcgagac
     5281 cccggcgggc gtgcccgtca ggatgacgtc acccggcagg agcgtcacga cctcgctcac
     5341 catcgccacg atctgcgcgg catcccagat catgtcgtgg gtgttgccga actggcgtcg
     5401 ctcgccgttc acccgcgcct cgatctccag atccccggtg tcgaggtcgg tctcgatcca
     5461 cggcccgagc ggcagggagg tgtcgaaggc cttgctgcgg aaccactggt tctcggcctt
     5521 ctgggcgtcg cgcgcggtca cgtcgttggc gcacgtgtag ccgtagatcg ccgcgagcgc
     5581 gccctcgggc gacacgtcct tgatgagtcg gccgatcacg accgcgagct ccgcctcgtg
     5641 ctgaacgtcc tcgctgtagt gcggcaggac gatggggtcg tcggggccga cgacggcggt
     5701 gttgggcttg aggaagagca ggggcaggtc gcccgacttg ccgacgttgc ccagttcctt
     5761 cgcgtgctcg gcgtagttcc ggcccatgca caccgccttc gagcggggaa tgatcggggc
     5821 gaggagccga agcccctcgg ccgagagcgg cacccgctcc cccgtcggct cgcccgggac
     5881 gtagagggga tcgcccgtga ggacgagcag ctcctcctcg cccggggcgc cctcgacctt
     5941 tgcgtagcga ggctccgaac ccgtggtgaa acgcgcgata cgcatgagcc caggttaccg
     6001 gtcggcgggg gcgaccccgg tagtctgacg ccatgcctca gcctgccgcg acgctcctcg
     6061 acgaactcgc ggcccgcgcg gacctccggc tggacacgct cgtcgcgatg atcgtggcga
     6121 acgggcttga ggaggcgagc cttggggacg cgtacctggg gcggcggggc gccgacgtcc
     6181 tggcacacct gcacggctgg cacggcctct tcgccgggtg gtgccgcgag gatggtcgcg
     6241 gcgacgagcc cacgcttccc gcgaccgggt acacgtggga ggaccttcgc gcgctgaacg
     6301 acgagatcta cgagcgctac cgcgccatgc catggacccg catccttgag gtcacgcggg
     6361 agtcgcacgc gacgatgatc gagttgctcc gaacgttccc cgttggcgcc ctcgccgacg
     6421 cctcgcggta cccctgggcg aacgggccgc tcatcgacct tgcggacgag tgtctcggca
     6481 agcactacga ctggggcatc gagcgcatcg aggagtacgt cagggctcat gagtagcaag
     6541 gctcggtagg gtcgtggggt gaacactcac gcctcgaccc ccgcgatcgc ggccctcgtc
     6601 cgcgccaacg tcgcccacac ccttcacccc tacgagcacg atccggggag cgacctcggc
     6661 tacggcctcg aggccgccgc ggcgatcggc gtggaaccct cgcgcgtctt caagaccctg
     6721 tgcgtcgagg cggacggagc cctcgcggtc gcgatcgtcc cggttgacgc gatgctcgac
     6781 ctcaagtcgc tcgcgtcggc gctcggcgcg aagaaggccg cgatggccga ggcggtcgcc
     6841 gccgagcgcg cgacggggta cgtcgtcggg ggcatctccc ccatcggcca gaagaggcgc
     6901 ctgaggaccg tgctcgactc gtcggcactg gccttcgacg ccgtgtatgt gagcgggggt
     6961 cgccgcgggc ttgacgtcgg cctcgccccg ggcgacctcg tcgccgtcac gggcgcgatc
     7021 accgcgccga tagcgaggga ctcgtgaccc ccggcgacga ggtttcgtcc gacgccgctt
     7081 cgcccgacgc ggtggcgccg tacgccgcgc gcctcgccga ggtgaaagcc cgggtgcgcg
     7141 cggcggaggc ggcgtgcgga cgcgcggggg ccggggtgcg ggtgctcgtc gcgacgaaga
     7201 cccgcgacga ggccacggcc gaggcggtgt gcgccgccgg cgcccggctc gtcggagaga
     7261 accgcgtcca ggagcttgtg gcgaaggggc cggtgttccg ggcggcgggg gtcgagaccc
     7321 acctgattgg gccgctccag cgaaacaagg cccgagacgc ggtcgcgtgg gcggattgca
     7381 tcgagacggt ggattcgctc gcgctcaccg agcggatctc gggcctcgcg ttcgaggcgg
     7441 gacggaccct cggcatcatg gtgcaggtga acgtctcggg cgagggaacc aag
//
LOCUS       DASVKP010000079         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_930694, whole genome shotgun sequence.
ACCESSION   DASVKP010000079 DASVKP010000000
VERSION     DASVKP010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930694"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VF362_01715"
     CDS             complement(<1..762)
                     /locus_tag="VF362_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7589675.1"
                     /translation="MTASIPSFAGARALITGGAGFIGSTLAIALVARGARVRVVDAMI
                     PGYGGNLFNLEPVKGRVAVEVADIRDPEAMSRAVRGQDFVFHLAGQVDHVLSLTDPFP
                     DIDINIKGTAVVMEALKHHNPKARVVYTGTRGQYGPAVTLPVAEDAPTNPRGIYEISN
                     LTAEKIIEVYDRVHGIPSVLLRLTNVYGPRAQMKHSRYGVVNWFVRLALDGSVIPVFG
                     DGTILRDFLYVDDCVEAILLAAGSDAVFGEVLNVGS"
     gene            complement(781..>2028)
                     /locus_tag="VF362_01720"
     CDS             complement(781..>2028)
                     /locus_tag="VF362_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589676.1"
                     /translation="FSGLTFAAALTAALRFQEENRPASLLAAALLLNAGLLTSDGAAP
                     AAVLAPLLFLATRGRADRRWAATSACWSLALVPWTVAFLRFLRDPGSYAAVALAPFDA
                     VRQGRLAAALTAHDFMPWRWAFRRPVWIEHLPSVVPGWAYVLAALAGAAAFVAAASAA
                     GAASGAAVRYVERWPRRRYVAGVLVLMIVATHAAFSGVQLAEVRYRTHLVTRLLASIL
                     VAMAADSLASRLRAPRLALALPAVFVGLGVAGGLERQDTYLATWRRQRTELASILEAA
                     PALDPKATLVLSLAPDPGGFQATRVPYLAASWASLLWDDPSIARRTVVALPRWGSDCR
                     PVAAGLSCAGGPGSGVHSWDRLVVLRFDPASFQYRLVREFPESGGAYRPYALIRPEPL
                     SARARALLEGPRFLGRLLPAGGA"
BASE COUNT          345 a          681 c          779 g          223 t
ORIGIN      
        1 actgcccacg ttcaggacct cgccgaatac cgcgtccgag cccgccgcga ggaggatggc
       61 ctcgacgcag tcgtcgacgt agagaaaatc gcgcaggatc gtcccgtcgc cgaagacggg
      121 gatcacggac ccgtcgagcg cgaggcgcac gaaccagttc acgacgccgt accgcgagtg
      181 cttcatctgc gcgcgcggcc cgtagacgtt cgtgagacga aggaggacgg acgggatccc
      241 gtggacgcgg tcgtagacct cgatgatctt ctcggccgtg agattcgaga tctcgtagat
      301 cccccgcggg ttcgtcggcg cgtcctccgc gaccgggagc gtcacggcgg gcccgtactg
      361 gccgcgcgta cccgtgtaga cgacgcgcgc cttcgggttg tggtgcttga gggcctccat
      421 gaccacggcc gtccccttga tgttgatgtc gatgtcgggg aacgggtccg taagcgagag
      481 gacgtggtcg acctggcccg cgaggtggaa gacgaagtcc tggccgcgca cggcccggct
      541 catcgcttcc gggtcgcgaa tgtccgcgac ctcgacggcg acgcggccct tcacgggctc
      601 gaggttgaag aggttgccgc cgtagccggg gatcatcgcg tcaacgacgc ggaccctcgc
      661 gccgcgcgcg acgagcgcga tcgcgagggt acttccgatg aaccccgcgc cgcccgtgat
      721 gagagcgcgc gcgcccgcga aggacgggat cgaggcggtc atggcgcggc gagcatagcg
      781 tcaggcacca cccgcgggca gcaggcggcc caggaagcgg ggcccctcga ggagcgcgcg
      841 cgcccgcgcc gaaaggggtt cgggacggat gagcgcatat ggccggtacg cgccgccgga
      901 ctccgggaac tccctgacga gccggtattg gaacgacgcc gggtcgaacc tcagcacgac
      961 gagccggtcc cacgagtgca cgcccgagcc gggaccgcct gcgcaggaca gaccggcggc
     1021 cacgggtctg caatcggacc cccagcgcgg cagcgcgacg acggttcgcc tggcgatgga
     1081 cgggtcgtcc cagagcagcg acgcccacga cgcggcgagg tacgggacac gcgtcgcctg
     1141 aaatccgccg gggtccggcg cgagggagag gacgagcgtc gctttcgggt cgagagcggg
     1201 cgcggcctcg aggatcgagg ccagctcggt gcgttgccgg cgccacgtcg caaggtacgt
     1261 atcctgccgc tcgaggcctc ccgcgacgcc gagaccgacg aagacggccg ggagggcgag
     1321 cgcgaggcgc ggcgcgcgca gccgcgacgc caggctgtcg gcggccatcg cgacgaggat
     1381 cgaggcgagg agccgcgtga cgaggtgcgt gcggtaccgc acctcggcta gctgcacgcc
     1441 cgagaacgcg gcgtgcgtcg cgacgatcat gaggacaagg acgcccgcga cgtagcgacg
     1501 gcgcggccag cgttccacgt agcggaccgc ggcccccgac gccgcacccg ccgccgacgc
     1561 tgccgccacg aatgcggcgg ccccggccag tgcggcgaga acgtaggccc acccgggaac
     1621 aaccgacggc aggtgctcga tccagacggg cctgcggaag gcccacctcc acggcatgaa
     1681 gtcgtgggcg gtgagcgcgg cggcgaggcg cccctgccgg accgcgtcga acggggcgag
     1741 cgcgaccgcc gcgtaggagc cgggatccct caggaaacgc agaaaggcga cggtccacgg
     1801 gacgagcgcg agcgaccagc aggcggacgt ggcggcccag cggcgatcgg cccggccccg
     1861 cgtggcaagg aacagaaggg gagcgagcac ggcggcgggg gccgctccat cggaagtgag
     1921 gagaccggcg ttcaggagga gcgccgcggc gagcagggag gcggggcggt tctcttcttg
     1981 gaagcgcagc gccgccgtga gtgcggcggc gaaagtcagg cccgagaa
//
LOCUS       DASVKP010000080         3345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_439761, whole genome shotgun sequence.
ACCESSION   DASVKP010000080 DASVKP010000000
VERSION     DASVKP010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439761"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VF362_01725"
     CDS             <1..467
                     /locus_tag="VF362_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="formate hydrogenase"
                     /protein_id="HEX7589677.1"
                     /translation="LADLASSADLASSADHAGHADLGAPSASRKEGDVLARFGIRVDE
                     FAASAEMVCGLTAGAERLEFPAARPNRAARPNRAAPSSPIRSGVGIVEGWRGTIVHRV
                     EVDPEGRLTRAAIVDPSWFNWPALPLAMADTILPDFPVTNKSFNLSYAGNDL"
     gene            complement(533..988)
                     /locus_tag="VF362_01730"
     CDS             complement(533..988)
                     /locus_tag="VF362_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006246584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HEX7589678.1"
                     /translation="MKIGELAESVGVSAKTVRYYESIGLLAEPERTATNYRDYEDAAA
                     DRLRFIRDSQAAGLTLAEVGSILEMKDAGQSTCGHTRDLVHRHLAEIDQQIAMLTQQR
                     EEMLALAERADAVDPGACTDPDRCQVIQASVGSAHQPGTRTRLALVGTP"
     gene            complement(1020..2015)
                     /locus_tag="VF362_01735"
     CDS             complement(1020..2015)
                     /locus_tag="VF362_01735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7589679.1"
                     /translation="MLSIRRVPHGDPTALRLWEDMWAEMGTRYGEMRSGAQLEPDGIV
                     ASLVGYVGDEAVGTVVVRFKDYPTIASAAEIKRMFVVPGRRGSGFARVLMGAAEEVAR
                     RAGATRIILETGTEQPEAVSLYRAIGYLPIPPYGSYPHDPRSLCFEKDLPTRVLVVSG
                     TMGAGKSAVASAVGDLLGARGARYGWIDGDALCQAGPANPRDPYNQGLLFDALAGVAP
                     AYRKRGLGIVIVARVVEDPDDRARYAHAFRSDGGSADVTIARVTAPEDVRLARIEARE
                     PEGQWREFGRARTVELEASLDALDLEDLAVENAERPAAETAAELLDAIGWRDETF"
     gene            2125..3171
                     /locus_tag="VF362_01740"
     CDS             2125..3171
                     /locus_tag="VF362_01740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX7589680.1"
                     /translation="MKSLGFLSFGHWSKGAGSRVRTASDSILATIDLAVAAEEVGMDG
                     AWIRVHHFEQNLSSPFPLLAAMAARTSRIELGTGVINMRYENPLYMAEVAGTADLISA
                     GRLQLGVSRGSPEVVADGPGAFGYPLPPGKLPVEDAAERIALFRRAIAGEGVAEPSPA
                     PYGSMPQLVNGLLAIVPVSPGLSERIWYGAGSIESARRVGELGMNLMSSTLVLEATGE
                     PFGTIQARQIEAYREAFKAAGHKREPRVSVSRSILPITTAEDNAYFGAIELQESTGEG
                     GDQIGIIDNTRATFGRTYVGAPDKIAAELAQDEGIAAADTLLITVPSQIGVEHNTHSF
                     EVIVKDVAPTLGWR"
BASE COUNT          502 a         1227 c         1136 g          480 t
ORIGIN      
        1 accttgccga ccttgccagc tccgccgacc ttgccagctc cgccgaccat gctggccacg
       61 cggacctcgg cgcacccagc gcctcgcgga aagaaggcga cgtcctcgcg cgcttcggca
      121 tcagggtcga cgagttcgcc gcctccgccg agatggtgtg cggactgacc gcgggcgccg
      181 agcgcctgga gtttcctgcg gcgcggccga accgcgctgc ccggccgaac cgcgcggcgc
      241 cgtcgtcgcc catccgcagc ggggtgggca tcgtcgaggg ctggaggggg acgatcgtcc
      301 accgtgtgga ggtagatccc gagggacgcc tcactcgcgc ggcgatcgtg gacccgtcgt
      361 ggttcaactg gcccgcgcta ccgctcgcga tggcggacac gatcttgccc gacttccctg
      421 tcaccaacaa gagcttcaac ctctcgtacg ccggaaacga cctctagtcc ctcggcgccg
      481 cctgcgctgg gccagggccc ctacggggtt cccaccggcc ccgccccaat ccctacgggg
      541 ttcccaccag cgcgaggcgc gtgcgcgtcc ccggctggtg cgcgctcccc acgctcgcct
      601 ggatgacctg gcagcggtcc ggatcggtgc acgcacccgg atccaccgcg tccgcgcgct
      661 cggcgagggc gagcatctct tcccgctgct gggtgagcat cgcgatctgc tgatcgatct
      721 ccgcgaggtg acggtgcaca aggtcccgag tgtgcccgca cgtcgactgg ccagcgtcct
      781 tcatctccag gatggagccc acctccgcga gcgtgagccc cgccgcctgg gagtcacgga
      841 tgaagcgcag ccggtcggcc gccgcatcct cgtagtcgcg atagttggtc gccgtgcgct
      901 cgggctcggc gagtaggccg atgctctcgt agtagcggac ggtcttggcg ctcacgccaa
      961 cgctctcggc gagttctccg atcttcatga ccccagcgta cgccgcacct tccatccggc
     1021 tagaacgtct cgtcccgcca gccgatcgcg tcgagcaact ctgcggcggt ctcggcggcg
     1081 ggcctttcgg cgttttcgac cgcgaggtcc tccagatcga gcgcgtcgag agacgcctcc
     1141 aactccacgg ttcgcgcgcg gccgaactca cgccactggc cctccggttc gcgcgcctca
     1201 atcctcgcga gccgcacgtc ctctggcgcg gtgacgcggg cgatcgtcac gtcggccgac
     1261 cccccgtcgg accggaacgc gtgggcgtag cgggcgcgat cgtcggggtc ctccacgacg
     1321 cgcgcgacga tcacgatgcc gagcccgcgc ttgcggtacg ccggggcgac gcccgcgagc
     1381 gcatcgaaca gcagcccctg gttgtacggg tcgcgcggat tcgcgggccc cgcctggcac
     1441 agggcgtccc cgtcgatcca cccgtatcgc gcgccccgcg cgcccagcag gtctcccacg
     1501 gcggacgcga ccgcggactt gcccgcgccc atcgtgccgc tgacgacgag gacgcgcgtg
     1561 ggcaggtcct tctcgaagca cagcgagcgg ggatcgtgcg ggtagctccc gtacggcggg
     1621 atggggaggt agccgatcgc ccggtacagc gagacggcct ctggttgctc cgtacccgtc
     1681 tcgaggatga tccgggtcgc gcccgcgcga cgcgccacct cctcggccgc gcccatgagg
     1741 acgcgggcga agccgcttcc ccggcggccg gggaccacga acatccgctt gatctcggcc
     1801 gcagaggcga tcgtggggta gtccttgaag cggacgacga cggtcccgac ggcctcgtcg
     1861 ccgacgtacc caacgagcga cgcgacgatc ccgtcgggct ccaactgggc gccgctcctc
     1921 atctccccgt agcgcgtgcc catctccgcc cacatgtctt cccacaggcg aagggccgtg
     1981 ggatcgccgt ggggcacgcg ccgaatggag agcacggcct cagcgtatcc acggcgcgcc
     2041 gcgaaggcgg gcgcgcacgc gggatgatcg cggcgttcgc cggcgttgac caggggcaat
     2101 cggtacgtca acgaggggtc ggccgtgaag agtctgggtt tcctgtcctt tgggcactgg
     2161 agcaagggag cgggctcccg cgtccggacc gcgagcgact cgatcctcgc gaccatcgac
     2221 ctggccgtcg ccgccgaaga ggtcggcatg gacggcgcgt ggatacgcgt ccaccacttc
     2281 gagcagaacc tctcgtcgcc gttccccctc ctcgccgcca tggcggcgcg cacgtcgcgc
     2341 atcgagctcg gcacgggcgt catcaacatg cgctacgaga acccgctgta catggcggag
     2401 gtcgccggaa cggcggacct catcagcgcc ggtcgcctcc agttaggggt ctcccgcggg
     2461 agccccgagg tcgtcgccga tggcccgggc gcgttcggct acccgctacc cccgggcaag
     2521 ctccccgtcg aggacgcggc cgaacgcatc gccctgttcc gcagggcgat cgcgggcgag
     2581 ggcgtcgccg agccgagccc cgcaccgtac ggaagcatgc cgcaactcgt gaacgggctc
     2641 cttgccatcg tgcccgtgag ccccgggctc tcggagcgca tctggtacgg cgccggcagc
     2701 atcgaaagcg ccaggcgcgt cggcgaactc ggcatgaacc tgatgtcgtc gaccctcgtc
     2761 ctcgaggcga ccggcgaacc cttcggcacc atccaggccc ggcagatcga ggcctaccgc
     2821 gaggcgttca aggcggcggg ccacaagcgc gagccgcggg tctcggtgag ccgtagcatc
     2881 ctgcccatca cgacggccga ggacaacgcg tacttcggtg cgatcgagct ccaggagtcg
     2941 acgggcgagg gcggagacca gatcgggatc atcgacaaca ctcgcgccac gttcgggcgc
     3001 acctacgtgg gggcgccgga caagatcgcg gcggaactcg ctcaggacga gggcatcgcg
     3061 gcggccgaca ccctcctcat caccgtgccg agccaaatcg gggtggagca caacacccac
     3121 tcgttcgagg tgatcgtgaa ggacgtcgcc ccaacgctgg ggtggcgata gcggcctcgc
     3181 ctatttccag gcccgctcgc gtcgacgggc gaggatgatg agcagtccac ccgagatcat
     3241 gagggcgagc gccacgcgaa cgggaaggat ggagaacccg gtcgcggcca acgtgcctgc
     3301 cgcaccgagc acgacgctcg agaagtctcc cgagcccgcg tcgtt
//
LOCUS       DASVKP010000081         7621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1262440, whole genome shotgun sequence.
ACCESSION   DASVKP010000081 DASVKP010000000
VERSION     DASVKP010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262440"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1126
                     /gene="pstC"
                     /locus_tag="VF362_01745"
     CDS             137..1126
                     /gene="pstC"
                     /locus_tag="VF362_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX7589681.1"
                     /translation="MTTVAPARSESTGDSLTAARSSLANAFFSRLTAGAASLILITLA
                     AVATFLVMRAWPAITSSSADLASKVDWLGTASSDTLIATIGYLVFGTLLIGTMALLIG
                     APISFGIALFISHYAPRRLAATLGYIVDLLAAVPSVVYGLWGGFVLVPKLAPFYGWLN
                     REFGWFPLFSGEVSPTGRVALSASIVLAIMILPIITAVTREVFLQTPILHEEAALAMG
                     ATRWEMIRMAVFPFARSGMIGATMLGLGRALGETMAVYMILSPGLSYSFHILDASKHN
                     TIAANIALKFPEANATGVEALIAMGLALFVITLGVNVLARWIVSRRSEFSGAN"
     gene            1128..2093
                     /gene="pstA"
                     /locus_tag="VF362_01750"
     CDS             1128..2093
                     /gene="pstA"
                     /locus_tag="VF362_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX7589682.1"
                     /translation="MATFATDGRSPLEELTARRRWTDRLMTVVIYASFVVAMIPLLWL
                     ATTVIQRGLVRFNLRADGTTGFNLEFLTTSMRGVFGGMPAGGIQAAIVGTLLITLAAT
                     VISVPIGMLTAIYIVEYGHGWLKRAVTFFVDVMTGIPSIVAGLFAYALFYVVVGPGTK
                     TGIVGAIALSVLMIPVVVRASEEMLRLVPNELREASYALGVPKWLTVLKVVVRTAFAG
                     LTTGVMLAVARVIGETAPLLVAVGVNDSVNANVFNGRMMSLPVYIVNEWGKNVQTCRA
                     SVAGCQPDIAIMRAWAAALVLILIVMALNLVARLIARYLSPKTGR"
     gene            2109..2888
                     /gene="pstB"
                     /locus_tag="VF362_01755"
     CDS             2109..2888
                     /gene="pstB"
                     /locus_tag="VF362_01755"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX7589683.1"
                     /translation="MAKRIDVNNLNVYYRAFCAVEGVSLAIEPNSVTALIGPSGCGKS
                     TFIRTINRMHEVIPGARVEGEVLMDGIDLYAPGIDPVAVRRHVGMVFQRPNPFPTMSI
                     GENVLAGVKLNNRRISKADAGEVVESSLKAANLWDEVKDRLDRPGSSLSGGQQQRLCI
                     ARAIAIKPDVLLMDEPCSALDPISTLAIEDLIAELKSEYTIVIVTHNMQQAARVSDRT
                     AFFNIAGAGKPGKLIEMNATSVIFSSPAERATEDYISGRFG"
     gene            2975..3457
                     /locus_tag="VF362_01760"
     CDS             2975..3457
                     /locus_tag="VF362_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX7589684.1"
                     /translation="MNFAVGETVIYPHHGAAEIQDISTRTIQGEEKMFLKLKVAQGDL
                     TITVPAENIDLVGVRDVVDAEGLKLVFTVLAEPYVEEPTNWSRRYKANVEKLASGDVI
                     RVSEVVRDLSRRDTDKGLSAGEKRMLSKARQVLVSELALAEKCSEEAAEARLDGVLAP
                     "
     gene            3454..4215
                     /locus_tag="VF362_01765"
     CDS             3454..4215
                     /locus_tag="VF362_01765"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HEX7589685.1"
                     /translation="MTVAAVVTAAGLGTRLGTDLPKALVRVEGRTLVAWAVERIAEVC
                     RDVVVTAPPAQLDAFGRAIAEAAISPDVAVAVIAGGETRQESVAAGVAELMSHSPRPT
                     IILVHDAARAFMPAEPMRVAIGAIEAGADGAVPIVPLVDTIVAVPGADGSLGLSVNRS
                     LLRAAQTPQAFRASALSDAHLRGAAEPGVTEPGVTEPAVTEPGAEVTDDAGLVRRYGY
                     RVVATEGHPWGFKVTVPGDLALADYIAKQARGAAS"
     gene            4245..4688
                     /gene="ispF"
                     /locus_tag="VF362_01770"
     CDS             4245..4688
                     /gene="ispF"
                     /locus_tag="VF362_01770"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005275120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX7589686.1"
                     /translation="MHAYGDAFGLSLAGLKWDDERVLSGHSDGDVVAHAACDALLSAA
                     SLGDLGSNFGVDRPEWEGAPGTALLKETARLVREAGFAIGNVAIQIIANRPRLGARRA
                     EAEAVLSEAIGAPVSISGTTSDGLGLTGRGEGVAAVATALITPVA"
     gene            4694..6109
                     /gene="cysS"
                     /locus_tag="VF362_01775"
     CDS             4694..6109
                     /gene="cysS"
                     /locus_tag="VF362_01775"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX7589687.1"
                     /translation="MTLRLFDSATRSVGDFAPLEPGKVGIYLCGATVQSAPHIGHMRA
                     AVAFDVLRRWLQRGGTQVTMIRNVTDVGDTIFAKAETAGVPWWEWALTNERLFTAGYD
                     ALGNLPPAYEPRATGHIPEMLVLIAQLIDSGHAYERDGSVYFDVRSFPAYGSLTNQAL
                     EDLAPAEDSPEAAKRDARDFALWKAVKADDPAGATWGSPWGPGRPGWHIECSAMARRY
                     LGGTFDIHGGGLDLRFPHHENEQAQSRAAGDGFARLWMHSAWVTQGGAKMSKSLGNTL
                     LVDAVLAEHPAAALRLALVGVHYRSMLEYGDATMDDAEATWSRLAGFVTRAGERVGQP
                     DAKAVATSVLPADFVEAMDDDLAVPRALAVVHEAVTEGNAALAAGDAAVAAAALLAVR
                     AMLDVLGLDPEAAPWAGQSGGATLDALDALVRAEIAARAAAREAKDWATADAIRDRLA
                     AAGITLEDGQEGVRWTLAGER"
     gene            6119..7069
                     /gene="rlmB"
                     /locus_tag="VF362_01780"
     CDS             6119..7069
                     /gene="rlmB"
                     /locus_tag="VF362_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX7589688.1"
                     /translation="MAGNSKRVGAVRKEGSKKGPQVGSGGKGRRALEGRGPTPKAEDR
                     PHHPAHRAKELADRASAKSPSRGPAPRKKVPGGPEVVAGRNAVLEALRAGVRAESLHI
                     FSRIEADDRVSELLHLALEADVPVIEVAKSRLDLLTGAAAHQGVALIVEPFRYLDLHD
                     LLAAPGLPLIVALDGIQDPRNLGAIIRSAAAFGATGVLLPERRAAGVTVAAWKTSAGA
                     VARMPIARVTNLARALEELKAEHIFTLGLAADGDVALADSGLLVEPLALVIGSEGKGL
                     SRLVREACDEVASIPISASTESLNASVAASLALYEVRKAR"
     gene            complement(7066..>7621)
                     /locus_tag="VF362_01785"
     CDS             complement(7066..>7621)
                     /locus_tag="VF362_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HEX7589689.1"
                     /translation="LNDLDEYRVVVAPPGEEESYAAHQWLTTVFEATVRAVPRKFRAK
                     LEPAQIFHEVLDHRWFLAEAAGRDVPMAVAVASYVKNILPERPDEKAVLGLTLAEMSA
                     ERTAEMTAELPALTEEVLTREIPQFEGDNTGAWGRDDGYGDDGYGDDDEDDEDDDAPV
                     QVVRKTTKRAAPKRTPPSAPKDSP"
BASE COUNT         1123 a         2675 c         2594 g         1229 t
ORIGIN      
        1 cgcactcgcc tcggtcaact cgatcaaggt cggctagggt ctgagggact agaccggtgg
       61 tgggtccatt cgtcaggatg gacccaccac cggaattcct ttggccgccc aacgaggtac
      121 gagacggaga acgcgcgtga ccactgtcgc cccagcgcgt tccgaaagca cgggcgacag
      181 cctcaccgcg gcgcggtcct ccctcgccaa cgcgttcttc tcgcgcctca cggctggcgc
      241 cgcatcgctc atcctcatca cgctcgccgc cgtcgccacc ttcctcgtca tgcgggcgtg
      301 gcccgcgatc acctcgagct ccgcggatct cgcgagcaag gtcgactggc tggggaccgc
      361 ctcgagcgac accctcatcg cgaccatcgg ctacctcgtc ttcgggacgc tgctcatcgg
      421 cacgatggcg ctgctcatcg gagcgcccat ctccttcggc atcgccctgt tcatctccca
      481 ctacgcgccg cggcggctcg ccgcgacgct cggctacatc gtcgacctgc tcgccgcggt
      541 cccgtcggtc gtctacgggc tctggggtgg attcgtgttg gtccccaagc tcgccccgtt
      601 ctacggctgg ctcaatcggg agtttggctg gttcccgctc ttctccggcg aggtatcgcc
      661 cacgggtcgc gtcgcgctct ccgcgtccat cgtgctcgcg atcatgatcc tgccgatcat
      721 cacggcggtg acccgggagg tcttcctcca gacgccgatc ctccatgagg aggccgcgct
      781 cgcgatgggg gcgacgcgct gggagatgat tcgcatggcc gtcttcccct tcgcccgatc
      841 ggggatgatc ggggcgacga tgctcggcct cgggcgcgcg ctgggggaga ccatggccgt
      901 ctacatgatt ctgtcgccgg ggctgtcgta ctcgttccac attctcgatg cgagcaagca
      961 caatacgatc gccgcgaata tcgcgctcaa gttccccgag gcgaacgcga cgggggtcga
     1021 ggccctcatc gcaatggggc tcgccctgtt cgtcattacg ctcggcgtca acgtcctcgc
     1081 gcgctggatc gtctcgcgtc ggtccgagtt ctcgggggcg aactgacgtg gccaccttcg
     1141 ccacggatgg acgctccccg ctcgaggagt tgacggcacg gcgcaggtgg accgaccgcc
     1201 tcatgaccgt ggtcatctac gcctccttcg tagtcgcgat gattcccctc ctttggctcg
     1261 cgaccacggt catccagcgg gggctcgtga ggttcaacct cagggccgat gggacgaccg
     1321 gcttcaacct tgagttcctc acaaccagca tgcgcggggt cttcggcgga atgcccgccg
     1381 gaggcatcca ggccgcgatc gtcggcaccc tgctcatcac gctcgcagcg acggtcatct
     1441 ccgtgccgat cggcatgctg acggcgatct acatcgtcga gtacgggcac ggttggctca
     1501 agcgggccgt gacgttcttc gtcgacgtca tgacgggtat cccctcgatc gtcgctgggc
     1561 tcttcgcgta cgcgctcttc tacgtcgtcg tgggcccggg gaccaagacg ggaatcgtgg
     1621 gggcgatcgc gctctcggtg ttgatgatcc ccgtcgtcgt tcgggcgagc gaggagatgc
     1681 tccgcctcgt cccgaacgag ttgcgcgagg cctcctacgc gctcggggtt cccaaatggc
     1741 tgaccgtgct caaggtggtg gttcgcaccg cgttcgcggg cctcacgacg ggcgtgatgc
     1801 tcgcggtcgc ccgcgtgatc ggcgagacgg cccccttgct cgtcgcggtc ggggtcaacg
     1861 actcggtgaa cgccaacgtg ttcaacgggc gcatgatgtc cctgcccgtg tacatcgtca
     1921 acgagtgggg caagaacgtt cagacgtgcc gcgcgtcggt ggccgggtgt cagccggata
     1981 tcgcgatcat gcgcgcatgg gccgccgcgc tcgtcctgat cctcatcgtc atggcgctca
     2041 acctcgttgc gcgcctcatc gcccgatacc tgtcccccaa gaccggccga tagaaacacg
     2101 gagacatcgt ggccaaacgc atcgacgtca acaacctcaa cgtctactac cgcgccttct
     2161 gcgccgtgga gggcgtgtcc ctggcgatcg agccgaactc ggtgaccgcg ctcatcggcc
     2221 cgtccgggtg cggcaagtcc accttcatcc ggacgatcaa ccgcatgcac gaggtgatcc
     2281 ccggcgcgcg cgtcgagggt gaggtgctga tggatggcat cgacctctac gctcctggca
     2341 tcgatcccgt cgcggtgcgg cgtcacgtcg gcatggtgtt ccagcggccc aacccattcc
     2401 cgacgatgtc gatcggcgag aacgtcctcg cgggcgtcaa gctcaacaac cggcgcatat
     2461 ccaaggccga cgccggggag gtcgtcgaga gttcgctcaa ggccgcgaac ctctgggatg
     2521 aggtcaagga ccgcctcgac cggcccggct cgtcgctgag cggcggccag caacagcgcc
     2581 tctgcatcgc ccgcgcgatc gcgatcaagc cagacgtcct gttgatggat gagccgtgct
     2641 ccgccctcga cccgatctcg accctcgcga tcgaggacct catcgccgaa ctcaagagcg
     2701 agtacacgat cgtcatcgtc acgcacaaca tgcagcaggc cgcgcgcgtg agcgacagga
     2761 ccgcgttctt caacatcgcg ggcgcgggca agccgggcaa gctgatcgag atgaacgcca
     2821 cgtcggtcat cttctcgagc cccgcggagc gagccacgga ggactacatc tcgggccgct
     2881 tcggctaggc gcgggcgccg gaatcagccc cctgatattg gcgaggtccc caatggtaga
     2941 atgggtgtcc gcgcagaggg gaaaccgagt tccgatgaac ttcgcagttg gcgagaccgt
     3001 catttacccg catcatgggg cggcggagat ccaggacatc agcactcgga ccatccaggg
     3061 cgaagagaag atgtttctca aactcaaggt tgcccagggc gatctgacga tcaccgttcc
     3121 cgcagagaac atcgacctcg ttggcgttcg cgatgtggtc gacgccgagg gcctcaagct
     3181 cgtgttcacc gtcctcgccg agccctatgt ggaggagccg accaactggt cgcgccgcta
     3241 caaggcgaac gtcgagaagc tcgcctccgg tgacgtgatc cgcgtgtcgg aggtcgttcg
     3301 cgacctctcg cgccgcgaca cggacaaggg gctgagcgcg ggcgagaagc ggatgctctc
     3361 caaggcgcgc caggtgctcg tttcggaact cgcactcgcg gagaagtgtt ccgaggaggc
     3421 tgcggaagcg cgcctcgacg gggtcctcgc gccgtgaccg tagcggcggt cgtcaccgcc
     3481 gcgggtctgg ggacgcgact cggaacggac ctcccgaagg ctcttgtgcg cgttgagggc
     3541 agaaccctcg tcgcctgggc cgtcgaacgg atcgcggagg tgtgccggga cgtcgtcgtc
     3601 acggcaccgc ccgcccagct cgacgcgttc gggcgggcca tcgccgaggc ggcgatctcg
     3661 ccggacgttg ccgtggccgt catcgccgga ggtgagacgc ggcaggaatc ggtcgccgcc
     3721 ggcgtcgccg aactgatgtc ccactcgccg cggccgacga tcatcctggt ccacgacgcg
     3781 gcccgcgcct tcatgcccgc cgagcccatg cgcgtcgcca tcggcgcgat cgaggcgggt
     3841 gcggacggcg cggttccgat cgtcccgctc gtggacacca tcgtcgcggt tcccggggcg
     3901 gacggtagcc tgggcctttc cgtcaatcgc tccttgctcc gggccgcaca gaccccccag
     3961 gcgtttcgcg cgagcgcgct gagcgacgcg cacctgcggg gcgcggccga acccggcgtc
     4021 accgaacccg gcgtcaccga acccgccgtc accgaaccgg gagcagaggt gaccgacgac
     4081 gccggcctcg ttcgtcggta cggttatcgt gtggtcgcga ccgaggggca cccgtgggga
     4141 ttcaaggtga cagtcccggg cgacctggcg ctcgcggact acatcgcgaa acaggcgcgg
     4201 ggggcggcgt cgtgacgacc ccgcgcacgg ggataggagt cgatgtgcat gcctacggcg
     4261 acgcctttgg cctctcgctc gcgggcctca agtgggacga cgagcgggtc ctgagcggcc
     4321 actccgacgg ggacgtcgtc gcccacgccg cgtgcgacgc gctgctatcc gccgccagcc
     4381 tcggggacct cggttctaac ttcggcgtgg atcgccccga gtgggagggc gcacccggaa
     4441 ccgcgctcct gaaggagacc gcgcgtcttg tgcgagaggc cggtttcgcg atcggcaacg
     4501 tcgcgattca gatcatcgcc aaccgtccgc ggctcggcgc tcgtcgcgcg gaggcggagg
     4561 cggtgctctc tgaggcgatc ggcgccccgg tatcgatctc ggggacgacg tcggacggcc
     4621 tcggcctgac cggcagggga gagggcgtcg cggctgtcgc cacggcgctc atcacgccgg
     4681 tagcctgaag cccgtgacgc tccgcctctt cgattcagcg acccgcagcg tcggcgattt
     4741 cgccccgctc gaaccgggca aggtcgggat ctacctctgc ggcgccacgg tgcagtccgc
     4801 gccccacatc gggcacatgc gcgccgcggt cgcgttcgac gtccttcgcc gctggctcca
     4861 gcgcggcggc acccaggtca cgatgatccg caacgtcacg gacgtcggcg acacgatctt
     4921 tgcgaaggcc gagactgcgg gggtcccgtg gtgggagtgg gcgctcacga acgagcgcct
     4981 cttcacggcg gggtacgacg ccctggggaa cctccctccc gcctacgagc cgcgcgctac
     5041 cggccacatc cccgagatgc tcgtcctcat cgcccaactc atcgactccg gccacgccta
     5101 cgagagggac ggctcggtct acttcgacgt ccgctccttc ccggcgtatg gctcgctcac
     5161 gaaccaggca ctcgaggacc tcgcgccggc ggaggactct cccgaggccg ccaagcgcga
     5221 cgcgcgcgat ttcgccctgt ggaaggccgt caaggcggac gacccggcgg gggcgacgtg
     5281 ggggagcccg tgggggccgg ggcgccccgg ttggcacatc gagtgctcgg ccatggctcg
     5341 gcgctacctc ggcgggacct tcgacatcca cggcggcggc ctcgacctgc gcttcccgca
     5401 ccacgagaac gagcaggcgc agtcccgcgc ggcgggcgac ggattcgccc ggctgtggat
     5461 gcactccgcc tgggtcactc agggcggcgc caaaatgtcc aagtccctcg gcaacaccct
     5521 cctggtggac gcggtgcttg cggagcaccc cgccgccgcc ctgcgactcg ccctcgtcgg
     5581 cgtccactac cgatccatgc tcgagtacgg cgacgcgacg atggacgacg ccgaggccac
     5641 gtggtctcgc ctcgccggat tcgtgacgcg agcgggggag cgggtggggc agcccgacgc
     5701 gaaggcggtg gcgacctccg ttcttcccgc cgacttcgtc gaagccatgg acgatgacct
     5761 tgccgttccc cgggccctag cggtcgttca cgaggcggtc accgagggaa acgcggccct
     5821 cgccgcgggg gacgcggccg tcgccgcagc cgcgcttctc gccgttcggg ccatgctgga
     5881 cgtgctcggg ctcgaccccg aggccgctcc ctgggccgga caatcgggcg gggccacgct
     5941 cgacgcgctt gatgcgctcg tgcgcgccga gattgccgca cgcgccgcgg cgcgcgaggc
     6001 gaaggattgg gcgacggccg acgcgattcg cgatcggctc gccgcggcgg gcatcacgct
     6061 cgaagacggt caagagggcg ttcgctggac cctcgcgggc gagcgctagg agagggccat
     6121 ggcaggcaat tcgaaacgcg tcggcgccgt ccgcaaggag ggctccaaga agggtccgca
     6181 ggtcgggagc ggcggcaagg gtcgccgcgc gctcgagggc aggggtccga cgcccaaggc
     6241 cgaagatcgc ccccaccacc ccgctcaccg cgccaaggaa ctcgcggacc gcgcgtccgc
     6301 gaagtcgccg agccgaggac cggcgcctcg aaagaaggtt cccggagggc ccgaggtcgt
     6361 cgcgggccgc aacgcggttc tggaggcgct gcgcgccggc gtgcgggcgg aaagcctgca
     6421 catcttctcc cggatcgagg ccgacgatcg cgtgagcgag ttgctccacc tcgccctcga
     6481 ggccgacgtc cccgtgatcg aggtcgcgaa gtcccgcctc gacctcctga ccggagccgc
     6541 cgcgcatcag ggtgtcgcgc tgatcgtcga gcccttccgc tacctcgacc tccacgacct
     6601 gctcgccgcc cccggcctcc ccctcatcgt cgcgctcgac gggattcagg atccgcgcaa
     6661 tctcggggcg atcatccggt ctgccgccgc gttcggcgcg acgggggtcc tccttccgga
     6721 gcgacgggcg gcgggggtga cggtcgcggc gtggaagacg tccgcgggcg cggtggcgcg
     6781 aatgccgatc gcgagggtga cgaacctcgc ccgcgcgctg gaggagctca aggccgagca
     6841 catcttcacc cttggcctcg cggccgatgg agacgtcgcc cttgccgaca gcggcctgct
     6901 cgtcgagccg ctcgcgctcg tcatcggctc ggagggcaag gggctctcgc gcctggtccg
     6961 cgaggcgtgc gacgaggtcg cgagcattcc gatctccgcg agcaccgagt cgctcaacgc
     7021 ctcggtggcg gcctcgctcg cgctttacga ggtcaggaag gcgcgctagg gggagtcctt
     7081 gggggccgac ggcggggtgc gcttcggagc cgctcgcttg gtggtcttcc gcacgacctg
     7141 cacgggggcg tcatcgtcct cgtcgtcctc gtcgtcgtcg ccatagccgt cgtcgccata
     7201 gccgtcgtcc ctgccccatg ccccggtgtt gtcgccctcg aactgaggga tctcccgcgt
     7261 caggacctct tccgtgagtg ccggaagctc ggcggtcatc tcagcggtcc gctcggccga
     7321 catctcggca agcgtgagcc cgaggacggc cttctcgtcg gggcgctccg gcaggatgtt
     7381 cttgacgtag gacgcgacgg cgaccgccat cgggacgtcg cgccccgcgg cctcggcgag
     7441 gaaccagcgg tgatccagca cctcatgaaa gatctgggcc ggctcgagct tggcgcggaa
     7501 cttcctgggg acggcgcgga cggtggcctc gaacacggtc gtgagccact ggtgcgccgc
     7561 gtagctctcc tcctcgcccg gaggcgccac gacgacccgg tattcgtcga ggtcgttgag
     7621 g
//
LOCUS       DASVKP010000082        10894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_604785, whole genome shotgun sequence.
ACCESSION   DASVKP010000082 DASVKP010000000
VERSION     DASVKP010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 10894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10894
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604785"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..790)
                     /locus_tag="VF362_01790"
     CDS             complement(170..790)
                     /locus_tag="VF362_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7589690.1"
                     /translation="MTPESAVVLAAAPEDARRTDTRQRILEVAARLFSERGFAGTSIR
                     DIADALGVTKAALYYHFPSKDAILGELVAQPLAAVRAVLAEPHRLTDRAGREVFIRRA
                     VDALSSCGPEMMAVFRDPKLLAAVDAEVEDSGIIHSVAATLAMGLSGVHRLSDVAPQH
                     MVRAIAAVAAGEAAIDNWRVVYPDCTHFSDADQEVIVRLIVATLES"
     gene            891..3119
                     /locus_tag="VF362_01795"
     CDS             891..3119
                     /locus_tag="VF362_01795"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX7589691.1"
                     /translation="MMEGSVKALAEFSTRRPLLVVLLWIAAVVGFQVASVIAGPDFRD
                     TLTLKASDSQAAYGLLAERFPELAGSSDTIAWRVGSGGVTDPAAKAFISGVLDQVASV
                     PDVAGVTSPFTPAGAGQISADGTIAFAQVQWDQSAHNLPITSVEKVGNLVSAANGTNG
                     LTVGLSGQAASRLTIPTLGRGEFIGLAIAALILFIAFGSLLATTVPLIGAIVAITGAI
                     GALGLVSNFGPIPSSAPMLIVLLGLGIGIDYALFITNRHRRGLKAGRPIRESVIGATT
                     TSGRAVLFAGATVFIALAGMYVPRISFLTGLATSAAITVVFSVAASITLVPALLQLYG
                     MRVLSRRERRALALDGGSASGAAPTPGLVSGFIGRHPIATSLAAFGLIVAVAGPALGL
                     RLGNADQGNDPSGTTTRTAYDIIAEGFGPGSNGPLLIAVDLTGATSATALTDLTARIA
                     ADPGVAAVIGPIPNAAGDAAVIQAIPTTNPQDAATVDLVGRIRNVYGAGARTEGLAVH
                     VGGAVASNVDFTSSIAGSLPLFFAVVVGLSMFVLALAFRSLILPLVGAVLNLLSAAAA
                     FGVIVAVFQWGWFHSIVGIGEGGPIEPFVPVILFALLFGLSMDYQVFLVSRIAELWHA
                     TGDNRLAVQRGLAEVSRVIVAAAAIMVAVFGSFVTSESRILKLLGLGLAAAVFLDAFV
                     IRVTLMPAVMRLLGRANWWMPRWLDRILPHVDLDKGDDATPGAPGHAEAAADRGLEPA
                     RA"
     gene            complement(3116..3409)
                     /locus_tag="VF362_01800"
     CDS             complement(3116..3409)
                     /locus_tag="VF362_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589692.1"
                     /translation="MGIFARRDRVADADFTFLSYADARLMRGLAAEAFARAGVQVKPR
                     GDHLVGPKGQLFGLYTLAAECHGIHEGPEAWVPAVEKHVRTLLASSGMGASGA"
     gene            complement(3539..4150)
                     /locus_tag="VF362_01805"
     CDS             complement(3539..4150)
                     /locus_tag="VF362_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX7589693.1"
                     /translation="MLGSSRLKPRYRGWLALVVGGLILLTGPWLCLHVATAGSVVEAG
                     EPVESAEAALVLGTLVNEDGTLSPRLAERVEVAAGLYLDGTVPRLVMSGTSESDTGQD
                     ETARMRDYAVSLGVPAGAITLAPVGLDTYATCDRAATVYGLHRVIVVTNEFHVARATW
                     LCQRAGLSAVGVHAPATPTGWTIRGNAREVLAAWKAVLDVVSR"
     gene            complement(4154..4399)
                     /locus_tag="VF362_01810"
     CDS             complement(4154..4399)
                     /locus_tag="VF362_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004658 - propionyl-CoA carboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase epsilon subunit"
                     /protein_id="HEX7589694.1"
                     /translation="MNEQDDADATAAQFHVVRGHVDDDELAALVAGVVAYAAAASGEA
                     ETPGSPTSAWMDRSRSMRGRRVGILGRGENAWRHSVR"
     gene            complement(4396..5991)
                     /locus_tag="VF362_01815"
     CDS             complement(4396..5991)
                     /locus_tag="VF362_01815"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019134911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX7589695.1"
                     /translation="MTAKRTSASKTTAGALDALKARVDAAVAAPEAEKQAKQHARGKK
                     SARERVLALLDEGSFVEIDALSRHRSRNFGLETNRPFGDGVVTGYGTIDNRQVAVYSQ
                     DFTVFGGSLGEVHGQKIAKTQDFALRTGVPLIGISDGGGARIQEGVAALTQFAEIFRR
                     NVAASGVIPQISIILGPSAGGAVYSPALTDFIVMADGTSQMFITGPDVIKSVTGEEVT
                     FEELGGGQTHNVKSGVAHFLASDEDDAIDYVKHLLQFLPQNNLTDPPAWDFDADLSLT
                     DEDRALDAIVPDSDNQPYDMKTVIEAVLDDGEFFEIQPLYATNIVVGFGHVEGHVVGV
                     VANQPKSMAGTLDINAAEKAARFVRTCDAFNIPVLTFVDVPGFLPGTDQEYQGIIRRG
                     AKLIYAYAEATVPLVTVITRKAYGGAYIVMASKQLGADVNLAWPTAQIAVMGASGAVN
                     ILQRRALAAVAEAGGDVEAERARLVAEYDDEIVNPYDAADRGYVDAVIEPSQTRAQIT
                     RALRALRTKRASLPPKKHGNIPL"
     gene            6030..6881
                     /locus_tag="VF362_01820"
     CDS             6030..6881
                     /locus_tag="VF362_01820"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX7589696.1"
                     /translation="MSVETRDSIGESRLALDGKQLGACLTWPSGPLARLTVVPESDST
                     NSDALTALRSGARVPHFSAFVSDFQRAGRGRSGRTWETPPGTSLTMSIVLRPEVARAS
                     FVWVPMLSGLAVVRALAGLGVSARLKWPNDVVVDSGDAEEVPGWGTDRKVAGILCEVE
                     LDAVVVGIGVNVSQRAEELPVAHATSLEVAGAASLARGALFGRIVRELDAIIVAWERE
                     PAMAHDLVARACATIGESVVVEIPGAAPLSGTAVGLSTDGGLEVRLAGGEMRTVLAGD
                     VRVRAVR"
     gene            6981..7538
                     /locus_tag="VF362_01825"
     CDS             6981..7538
                     /locus_tag="VF362_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589697.1"
                     /translation="MRLDRMRGRLFNAETRRPWTAAAVGAAIFTPMFWLSVATGLGKF
                     TAAHGQPLGAYPAAVVGLYFATILLIAPLLSLTAGYLLDRATSAWRRDDAVAVFLAFG
                     VVLAAAAVSFVMALAPGGGAFVPALWLFGLPLLVTLGVTRSVIDAVLASRKWAVAAVA
                     VAYAPALLAALWLLGLYVSHATVPA"
     gene            complement(7558..8265)
                     /locus_tag="VF362_01830"
     CDS             complement(7558..8265)
                     /locus_tag="VF362_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX7589698.1"
                     /translation="MCARYANFLDEQDLLDAFAVAEAAEDARLLPPSWNIGPMHLAPI
                     VALAPIGGDVAAPRHRRLIAARWGLVPTWAKDSSMGAKMFNARSETVTEKPSFRAAFA
                     QRRCLVPASGYYEWQARKGGKQPYFIYPEDASPLAFAGLWETRKDSDVRTFTFSILTT
                     AARGGLAAIHDRQPVMLRADERDAWMDVDSSADELMAVMASPSPAMAIRPVGKAVGNI
                     RNDDPSLVTAVAEPATE"
     gene            8672..9664
                     /locus_tag="VF362_01835"
     CDS             8672..9664
                     /locus_tag="VF362_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7589699.1"
                     /translation="MKKKLVTGVLMAGAMALALAGCSSNDTTTSAGGSTAPTAGKVIT
                     VGFAQTGSESGWRAANTESMKTAFSEANGFKLIFNAADNKPEAQIAAVHSFINQKVDA
                     IVIAAVVTTGWDDVLKEAQAAKIPVILEDRTIDADPSLYAAWFGDDFKAEGKTAGEWA
                     VKTFGSTPTNMVVLEGTTGSSAATDRTTGFNAAIAGSGIVQMASQTGDFTRAGGKTVM
                     EGFLQKYGKTIQLLFAQNDDMGLGAIDAIEAAGLVPGKDIKIVTIDATKDGMTALSVG
                     KINYIVECQPLLGDQVAKTVKDVLAGKTVEKMQYGVDGAFDQAQALAALPTRKY"
     gene            9812..>10894
                     /locus_tag="VF362_01840"
     CDS             9812..>10894
                     /locus_tag="VF362_01840"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX7589700.1"
                     /translation="MRNISIEFPGVKALDGVDLTLRAGEIHSLLGENGAGKSTLIKAL
                     TGVYAIDSGTIHVAGAKRTFHGTADAQEAGVAPVYQEINLLPNLSVGENVMLGHEVRG
                     KFGINWKATHEKARTYLKELGLDVDPRSLLASHSIAVQQLVAISRAVVIDCRLLVLDE
                     PTSSLDRSEVEQLFAVMRRLRERGVAILFVSHFLDQVYEISDTMTILRNGKLEGEYPI
                     NALNKKQLIEKMLGRELAELDSISRAATRKIDRTGEPILKVLALGRKGAIEPIDLDVF
                     DGEVLGLAGLLGSGRTELARIIYGADHADHGAMEVRGKHVRFARPRQAIDHRIAFSSE
                     DRRAEGAIADLTVAENIVLGVQAKRGW"
BASE COUNT         1618 a         3668 c         3789 g         1819 t
ORIGIN      
        1 cagggcccgg cggcgacggc gggcaggaac acgagcagcg cgacgatcca gcggtagcgc
       61 ggtcggattc gggagcgcgg ccggatgcgc gtgcgcggac ggctcacgga cgaaggcctc
      121 acgccgtcga gcctacggcc gctcgcgtcc tgccgcgcga ggagggaggc taactctcga
      181 gtgtggcgac gatgagtcgg acgatgacct cttggtcggc gtccgaaaag tgcgtgcagt
      241 ccggatacac gacgcgccag ttgtcgatcg ccgcttctcc tgccgcaacc gccgcaatcg
      301 cgcgaaccat gtgttgcggc gcgacgtccg agaggcgatg gacccccgac agccccatcg
      361 cgagcgtcgc ggcgacgctg tggataatcc ccgagtcttc gacctcggcg tcgacggccg
      421 cgagaagctt gggatcccgg aagaccgcca tcatctcggg gccgcacgag gacagcgcgt
      481 cgacggccct gcgaatgaag acttctcggc ctgcgcggtc ggtgaggcga tggggctccg
      541 cgaggacggc ccgaaccgcc gcgagcggct gggcgacgag ttcaccgagg atcgcgtcct
      601 tggaggggaa gtgatagtag agggcggcct tcgtcacgcc gagcgcgtcc gcaatgtcgc
      661 ggatcgacgt tccggcgaat ccccgctccg agaagagccg tgccgcgacc tcaaggatcc
      721 gctgacgggt atcagtgcgg cgggcgtcct caggggcggc cgcgagaacc accgcagact
      781 ctggtgtcac cgttcacctc cgttgagtga acttcggggc acttcacttg acgatcgttc
      841 attcagtatg aaacgatctc cttcgcgtcc accttaccgg tcgtttagtc atgatggaag
      901 gctccgtgaa agccctcgcc gaattctcca cccgtcgccc ccttctcgtc gtgctcctgt
      961 ggatcgcggc ggtggtcggg ttccaggtcg caagcgtgat cgcgggaccc gacttccgcg
     1021 acacgctcac gctgaaggcc tcggatagcc aggcggcgta cggactgctg gccgagcgat
     1081 tcccggaact tgcgggcagt tcggacacca tcgcctggcg cgtcgggagt ggcggcgtca
     1141 ccgatcccgc ggcgaaggca ttcatctcgg gcgtcctcga tcaggtcgcc tcggtcccgg
     1201 atgtcgcggg tgtcacgagc ccgttcaccc ccgccggtgc ggggcaaatc agtgcggacg
     1261 gcacgattgc cttcgcccag gttcagtggg atcagtccgc gcacaacctt ccgatcacct
     1321 cggtggagaa ggtcgggaac ctcgtctcgg ccgcgaacgg cacgaacggc ctcacggtcg
     1381 ggctgagcgg tcaggcggcc tcgcggctga ccataccgac gctcggtcgg ggcgaattca
     1441 tcggcctcgc aatcgcggcc ctcatcctct tcatcgcctt cggctccctg ctcgccacga
     1501 cggttccgct gatcggggcg atcgtcgcca ttacgggagc catcggggcg ctcggactcg
     1561 tgtccaactt cgggccgatc ccgagttctg cgccaatgct catcgtcctg cttgggctcg
     1621 gaatcggcat cgactacgcg ctcttcatca ccaaccggca ccgcaggggg ctcaaggcag
     1681 gccggccgat acgcgagagt gtgatcggcg cgaccacgac gtcgggtcgc gccgtgctct
     1741 tcgccggcgc gacggtgttc atcgcgctcg cgggcatgta cgtgccgagg atctccttcc
     1801 tcaccgggct cgccacctcg gcggcgatca cggtggtgtt ctcggtcgca gcgtcgatca
     1861 ccctggttcc cgcgcttctc cagctgtacg gcatgcgcgt gctcagtcgg cgcgagaggc
     1921 gcgcgctcgc gttggacgga gggagcgcct ctggcgcggc cccgacgccc gggctcgtgt
     1981 ccggcttcat cgggcgccac cccatcgcga ccagcctcgc cgccttcggg ctgatcgtcg
     2041 ccgtcgccgg tcctgcgctc ggccttcggc tgggcaatgc cgaccagggc aacgacccct
     2101 cgggcaccac cacccgcacc gcctacgaca tcatcgccga gggcttcggc cccggctcga
     2161 acgggccact cctgatcgcc gtcgacctta ctggcgcgac ctccgcgacg gcgctgaccg
     2221 acctcacagc ccgcatcgcg gccgaccccg gtgtggccgc ggtcattggc cccatcccca
     2281 acgctgcggg cgatgccgcg gtcatccagg ccatcccgac caccaacccg caggacgcgg
     2341 cgacggtgga tctcgtcggg cgaatccgga acgtgtacgg agcgggcgcc cgcacggagg
     2401 gcctcgcggt ccacgtcggg ggagcggtgg ccagcaacgt cgacttcacg agctcgatcg
     2461 ccggttcgtt gcccctcttc ttcgccgtcg tggtagggct gtcgatgttc gtcctcgccc
     2521 tcgcgttccg ctctctgatc ctgccgctcg tcggcgcggt gctcaacctc ttgtccgccg
     2581 ccgcggcgtt cggggtgatc gtggcggtgt tccagtgggg gtggttccac tcgatcgtcg
     2641 gcatcggaga gggcgggccg atcgagccct tcgtgcccgt gatcctgttt gcgctgctgt
     2701 tcgggttgtc catggactac caggtcttcc ttgtctcgcg catcgcggag ctctggcacg
     2761 cgaccggcga caaccgcctc gcggtgcaaa ggggcctggc ggaggtgtcc agggtgatcg
     2821 tcgcggccgc ggcgatcatg gtcgccgtgt ttggatcctt cgtcacctcg gaatcgcgga
     2881 tactcaagtt gctcggcctc gggctggcgg ctgccgtctt cctcgacgcc ttcgtcatcc
     2941 gcgtgacgct catgcccgcg gtgatgcggc tcctcggacg cgccaactgg tggatgcctc
     3001 ggtggctcga ccgcatcctg ccgcacgtcg acctcgacaa gggagacgat gcgaccccgg
     3061 gcgccccggg ccacgcggag gccgccgccg accgcggact cgagcccgct cgcgcctagg
     3121 cgccgctcgc gcccattccg gaggaggcca gcaacgtcct gacgtgcttc tcgacggccg
     3181 gtacccaggc ctcggggccc tcgtggattc cgtggcactc cgctgcgaga gtgtagagcc
     3241 cgaagagttg acccttgggc cccaccaggt ggtcaccccg cggcttgact tggacgcccg
     3301 cgcgcgcgaa ggcctcggct gcgaggcccc tcatgagccg cgcgtcggcg taagaaagaa
     3361 aagtgaagtc ggcgtccgcg acgcggtccc gccttgcgaa gatccccatc cccgcatctt
     3421 gccaaaggtc gcgcggtttc gcgcgtcgaa ggggatgggc ccttagttgc cgcaagcgtg
     3481 ggatgtggct ggcgacggga gtgacgccac gccgcgacat cgcgggcgac tgccctcctc
     3541 accgcgacac gacgtcgagc acggccttcc acgcggccag gacttcgcgc gcgttgccgc
     3601 gaatcgtcca tccggtgggc gttgccgggg catggacgcc caccgccgag aggcccgcgc
     3661 gctggcacag ccacgtcgcg cgcgcgacgt ggaactcgtt tgtcacgacg atcacgcggt
     3721 ggaggccgta cacggtcgcg gcgcgatcgc acgtggcgta ggtatcgaga ccgacggggg
     3781 cgagcgtgat cgctcccgcc gggacgccca gcgagacggc atagtcgcgc attcgtgcgg
     3841 tctcgtcctg cccggtgtca ctctcggagg tgccggacat gacgagcctc ggcacggtgc
     3901 cgtcgaggta gagccccgcc gccacttcca cgcgttctgc gaggcgaggt gagagtgtgc
     3961 cgtcttcgtt cacgagtgtt cccaacacga gcgccgcctc ggccgactcg acgggctcgc
     4021 ccgcctcgac caccgatccc gccgtggcga catgaaggca cagccagggg ccggtgagca
     4081 ggatcagtcc gccgacgaca agggcgagcc agccacggta gcggggcttc aggcgcgatg
     4141 aaccgagcat gcgctagcga accgagtgac gccaggcgtt ctcgccccgg ccgaggatcc
     4201 cgaccctgcg gccgcgcatc gatcgcgagc ggtccatcca cgccgaggtc ggggagccgg
     4261 gagtctccgc ctcgccgctg gcggcggccg cgtaggcgac cacgccggcc acgagggccg
     4321 cgagttcgtc gtcgtccacg tggccgcgca ccacgtggaa ctgggcggcg gtcgcgtcgg
     4381 cgtcgtcctg ttcgttcaca gtgggatgtt tccgtgcttc ttgggtggca gggaggcgcg
     4441 cttggttcgg agggcccgga gggcccgggt gatctgcgcg cgcgtctgcg acggctcgat
     4501 caccgcatcg acgtagcccc gatcggcggc gtcgtacgga ttgacgatct cgtcgtcgta
     4561 ttcggcaacg aggcgcgccc gctcggcctc gacgtcgcca cccgcctccg cgacggcggc
     4621 gagcgcccgt cgctgcagga tgttgaccgc cccgctggcg cccatcaccg cgatctgcgc
     4681 ggtgggccag gcgaggttga cgtccgcgcc gagttgcttg gaggccatga cgatgtaggc
     4741 gccgccgtag gccttgcgcg tgatgacggt gacgagcggg acggtcgctt cggcgtaggc
     4801 gtagatgagt ttggcgccgc ggcggatgat gccctggtac tcctggtcgg tgccgggaag
     4861 gaatccggga acgtccacga aggtcaggac cgggatgttg aacgcgtcgc aggtccgcac
     4921 gaaccgcgcc gccttctccg ccgcgttgat gtcgagggtc cccgccatcg acttcggctg
     4981 gttcgcgacg acgcctacca cgtggccctc gacgtggccg aagccgacga cgatgttcgt
     5041 cgcgtagagc ggctggatct cgaagaactc cccgtcgtcg aggacggcct cgatcaccgt
     5101 cttcatgtcg tagggctggt tgtcgctgtc gggaacgatg gcgtcgagcg cgcggtcctc
     5161 gtcggtgagg ctgaggtccg cgtcgaagtc ccacgcgggc ggatcggtga ggttgttctg
     5221 cgggaggaac tgcaacaggt gtttcacgta gtcgatggca tcgtcctcgt cggacgcgag
     5281 gaagtgcgcc acgcccgact tcacgttgtg cgtctgcccg ccgccaagtt cctcgaacgt
     5341 cacctcttcg cccgtcacgg acttgatgac gtcgggcccc gtgatgaaca tctgggacgt
     5401 gccgtccgcc atcacgatga agtcggtcag cgcgggggag tacacggctc ctccggcaga
     5461 gggaccgagg atgatcgaga tctgggggat cacgccggag gcggcgacgt tgcgccggaa
     5521 gatctccgcg aattgggtga gggccgcgac gccctcctgg atgcgggccc cgccgccgtc
     5581 ggaaatgccg atgaggggga cgcccgtccg cagcgcgaag tcctgggtct tggcgatctt
     5641 ctgcccgtgc acctcgccga gcgagccgcc gaacaccgtg aagtcctggg agtagaccgc
     5701 cacctggcgg ttgtcgatcg tcccgtaacc ggtgacgacg ccgtcgccga acgggcggtt
     5761 cgtctcgagg ccgaagttgc gggagcggtg gcggctgagc gcgtcgatct cgacgaagga
     5821 gccctcgtcg agcagggcga ggacgcgctc gcgggcgctc ttcttcccgc gcgcgtgttg
     5881 cttcgcctgc ttctcggctt ccggggccgc gacggcagcg tcgacgcgcg ccttgagcgc
     5941 gtcgagcgcg cccgccgtgg tcttcgacgc gctcgttctt tttgcggtca cgttgtgcac
     6001 cttcctcgat acggagttag cctagtcgcg tgagcgtaga gacccgcgat tcgatcgggg
     6061 aatctcggct cgcgctggac gggaagcaac tgggggcctg tctcacgtgg ccgagcggcc
     6121 ccttggcgcg cctgaccgtc gtccccgagt cggactcgac caactccgac gccctcacgg
     6181 cactccgctc gggcgcgcgg gtgccacact tcagcgcgtt cgtgtccgac ttccaacgcg
     6241 ccgggcgcgg gcggagcggc cgaacgtggg agacgccacc ggggacgtcc ctgacgatga
     6301 gcatcgtgct gcggcccgag gtcgcgcgcg cgtccttcgt gtgggttccg atgctctcgg
     6361 ggctcgcggt cgtgcgtgcg ctcgcggggc tcggcgtgag cgcgcggctc aagtggccga
     6421 acgacgtggt ggtggactcc ggcgacgcgg aggaggtccc cggttggggc accgaccgca
     6481 aggtcgccgg catcctgtgc gaggtcgaac tcgacgccgt ggtggtgggc atcggcgtca
     6541 acgtctctca gagggccgaa gagttgcccg tcgcccacgc aacgtcgctc gaggtcgcgg
     6601 gcgcggcctc ccttgcgagg ggcgcgctgt tcggccgcat cgtccgcgaa ctcgacgcga
     6661 tcatcgtggc gtgggagcgc gagcccgcga tggcgcacga ccttgtggcg cgggcctgcg
     6721 cgaccatcgg cgagagcgtc gtcgtggaga tccccggcgc ggccccgctg tcggggacgg
     6781 ccgtggggct ttccaccgat ggcggccttg aggtgcgcct ggcgggcggc gaaatgcgca
     6841 ccgtcctcgc cggcgacgtt cgggtgcgcg ccgtccgcta gggctccgac gacgagcgcc
     6901 acggcggcaa ggacggagac gaggtgccgg attcccgttc ggggtggcgt gttcgccctg
     6961 atcgcacgcg cctcgaccgg gtgcgcctcg accgcatgcg cggccgcctc ttcaacgccg
     7021 agacgcgacg gccctggacg gccgcggccg tcggcgccgc gatcttcacg ccgatgttct
     7081 ggctgagcgt cgccacgggc cttggcaagt tcaccgcggc gcacggtcag ccgctcgggg
     7141 cataccccgc ggcggtggtg ggcctctact tcgcgacgat cctcctcatc gcaccgctac
     7201 tctcgctcac ggctggctac ctcttggatc gcgccacgtc ggcgtggcgt cgcgacgacg
     7261 ccgtcgccgt gttcctcgcg ttcggcgtcg tgctcgccgc cgcggccgtg tcgttcgtca
     7321 tggcgctcgc gccggggggc ggggccttcg tgcccgcgct gtggctcttc ggccttccgc
     7381 tgctcgtgac gctcggggtg acgcggtccg tcatcgacgc cgtcttggcg tcgcggaagt
     7441 gggcggtcgc ggcggttgcg gtcgcgtacg cgcccgcgct actcgcggcg ctctggctcc
     7501 tcgggctgta cgtgagccac gcgacggtcc cggcctagtt actcggtgcc cgcccaacta
     7561 ctccgtggcg ggctcggcga ccgcggtgac caggctaggg tcgtcattcc ggatgttgcc
     7621 cacggctttc ccaacgggcc tgatcgccat cgcgggcgac ggggaagcca tcacggccat
     7681 gagctcgtcg gcggaggagt cgacgtccat ccacgcatcc cgctcgtctg cgcggagcat
     7741 cacgggctgg cggtcgtgga tggccgcgag gccgccgcgc gccgcggtgg tcaggatgct
     7801 gaacgtgaac gtccgcacgt cggagtcctt gcgcgtctcc cacaggccgg cgaaggcgag
     7861 cggcgacgca tcctcggggt agatgaagta gggctgcttg ccgcccttgc gcgcctgcca
     7921 ctcgtagtag ccgctggcgg ggacgaggca acggcgttgc gcgaaggcgg cgcgaaacga
     7981 cggcttctcc gtgaccgtct ccgagcgggc gttgaacatc ttggcgccca tcgaggagtc
     8041 cttggcccag gtgggaacca ggccccagcg ggcggcgatc aggcgccgat ggcggggggc
     8101 ggccacatcc cctccgatgg gggcgagcgc gacgatgggc gcgagatgca tgggcccgat
     8161 gttccacgac ggcgggagca ggcgcgcgtc ctcggcggcc tcggcgacgg cgaacgcgtc
     8221 gaggaggtct tgctcgtcga ggaagttcgc gtagcgggcg cacatgagga cagcctgtca
     8281 cgagcgcgca atcctggggg agggatcgat gcggctggcc gccctggccc ggattgcggg
     8341 ccgaattacg gccgtgttat ccctcacatt tgcggtccgg agcggggtgc tgttcgcgac
     8401 gccgatcgcc ggattggcat tgcggcgacg caccgccttc cgggccgaac cttgcccgtg
     8461 ttatccctca catcgtcgcg tcgcaggcgt gacgagcggg ttgttggggg cggagcccgc
     8521 agggaaggtc cccctgcgtt acgcaattgt gatcctcgag acgttgcggc ggcgctggat
     8581 gttagggcta acatggggtc gctaccctgc aaggatgcag gacgtgagtt gcccggctct
     8641 tggacgggcc ccagacacgg aggttttggg aatgaagaag aaactcgtaa ccggagtgct
     8701 gatggctgga gctatggctc ttgccctcgc agggtgctcc tcgaatgaca ccaccacctc
     8761 ggcgggcggc tctacggctc ccaccgcagg caaggtcatc accgttggct tcgcacagac
     8821 cggctccgag agcggctggc gcgcggccaa caccgagtcg atgaagacgg cgttctctga
     8881 ggccaacggc ttcaagctga tcttcaacgc tgcggacaac aagccggaag cccagattgc
     8941 ggccgtgcac agcttcatca accagaaggt cgacgcgatc gtcatcgcgg cggttgtcac
     9001 caccggttgg gacgacgtgc tcaaggaagc tcaggcggcg aagattccgg tcattcttga
     9061 ggaccgtacg atcgacgctg accccagcct ctacgcggca tggttcggcg atgacttcaa
     9121 ggcggaaggc aagaccgctg gcgagtgggc agtcaagacg tttggctcca ccccgaccaa
     9181 catggtcgtg cttgagggca cgaccggttc ctctgcggcg accgaccgta cgactggctt
     9241 caacgcggcc atcgctggaa gcgggatcgt ccagatggcc tcgcagactg gtgacttcac
     9301 ccgtgccggc ggcaagaccg tcatggaagg cttcctccag aagtacggca agacgatcca
     9361 actcctgttc gcgcagaatg acgacatggg tcttggcgcc atcgacgcca tcgaggcggc
     9421 cggactcgtt cctggcaagg acatcaagat cgtcacgatc gacgccacga aggacggcat
     9481 gacggctctc tccgttggca agatcaacta catcgtcgag tgccagccgc tcctcggtga
     9541 ccaggttgcc aagaccgtga aggacgtcct cgcgggcaag acggtcgaga agatgcagta
     9601 cggcgtggac ggtgcgttcg accaggcaca ggccctcgcg gccctgccga cccgcaagta
     9661 ctagaaagta gttggcgcgg ccgctgtttg ggtcgcacgc tggcggggcg agtctctcgc
     9721 cccgccagct ctctaccctt catcaggcgg cgctgactgc ctcaaagtag aagagaagct
     9781 gatcgtggtc gacaacagtg tggtcgtcga aatgcggaac atctccatcg agttccctgg
     9841 ggtcaaggcg cttgacggtg tggacctcac cctgcgcgcc ggcgagatcc actcccttct
     9901 cggggagaac ggagctggca agtccaccct catcaaggcg ctgaccggcg tctacgccat
     9961 cgactcgggg accatccacg tcgccggcgc caagaggacg tttcacggga cggctgacgc
    10021 ccaggaagcg ggcgtcgcgc cggtgtatca ggagatcaat ctcctcccca atctgagcgt
    10081 cggcgagaac gtcatgctcg ggcacgaggt ccgcggcaag ttcggaatca actggaaggc
    10141 cacccacgag aaggctcgca cctacctcaa ggagctcggc ctcgatgtcg atccgcggtc
    10201 gctcctcgcg agtcactcga tcgcggtgca gcaactcgtc gccatcagcc gcgcggtcgt
    10261 catcgactgc cgcctcctcg tgctcgacga gccgacctca agcctcgacc gttccgaggt
    10321 ggagcaactc ttcgccgtga tgcgccgctt gcgtgagcgc ggcgtggcca tcctctttgt
    10381 gtctcacttc ctcgaccagg tgtacgagat ctcggacacc atgacgatcc tccgcaacgg
    10441 caagctcgag ggtgagtacc ccatcaacgc actgaacaag aagcagttga tcgagaagat
    10501 gctgggccgc gagttggcgg aactcgactc gatcagccgc gcggcgaccc gcaagatcga
    10561 caggaccggg gagccgatcc tgaaggtgct cgcgctaggg cgcaagggcg cgatcgagcc
    10621 cattgacctt gacgtcttcg acggcgaggt tctcggcctg gcgggcctgc tcggctccgg
    10681 gcgaacggag ctcgcccgca tcatctatgg cgccgatcac gccgaccacg gcgcgatgga
    10741 ggtgcgtggc aagcacgttc gcttcgccag gccgcgccag gccatcgacc accggatcgc
    10801 attctcgtcg gaggaccggc gagccgaagg ggccatcgcc gacctcaccg tcgcagagaa
    10861 catcgtgctc ggagtccagg cgaagcgggg gtgg
//
LOCUS       DASVKP010000083         4851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_605286, whole genome shotgun sequence.
ACCESSION   DASVKP010000083 DASVKP010000000
VERSION     DASVKP010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605286"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VF362_01845"
     CDS             <1..378
                     /locus_tag="VF362_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589701.1"
                     /translation="DMASLSGSDVEATSLEEARYTLTYAYSGGGDAECKLWDAATNEP
                     VYFLKGANFAHAFSLVDLYTGAVAATKVFGSTAMKCCTSGTYDPSMFDGRHHRVGECV
                     GGSEPESTYSESELEAWLESQTD"
     gene            389..1822
                     /locus_tag="VF362_01850"
     CDS             389..1822
                     /locus_tag="VF362_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589702.1"
                     /translation="MPTAKSGGEAEKPDAKGSAAAPTAKRETPAIRKSRSAGDEGAST
                     TGKNAGAKGTPAKTADGGEEASTTGKNAGAKGTPAKTADAGEEAPKRRWFRSPTILGL
                     AGGVVLAACLTVFALLVFGGQRDVARIAAGGFHTCAIATSGSVECWGWNEHGQLGNGT
                     TESGGEPTVVEGSAGAIEVAAGAAHTCAILQDHSLWCWGWNVSGQLGDRSTLDRPTPV
                     EVSGLADVRQVSAGTHATCAVTSDGSAWCWGWNQSGQLGDGTEVGRSGPVLVKDLLDV
                     VQVSVGDDHACAVVGDGEVWCWGSNTAGEVGATAAQHQTSPVKVEGLAGAVKVAVGGA
                     HSCAILNDGSVSCWGDNAFGQLGGPWYTNGSGGPVAVLGLEGVTALALGYEHSCAILD
                     GGSVSCWGGNDHGQVGDGSATDRSGPVDVEGIEDAKQISAGDFHTCVLRGNGEVSCWG
                     ADADGQLGDGAAEDRDQPVTVIGLRAG"
     gene            1868..2464
                     /locus_tag="VF362_01855"
     CDS             1868..2464
                     /locus_tag="VF362_01855"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7589703.1"
                     /translation="MTRVFLLRHGETDWNATGRLQGSTDVPMNARGLEQVGAAARALG
                     ARLPQGTVVVSSPLVRARATAEALAALTGATVVYDDRMKERSYGAWEGMTAAEREAHD
                     SVEVARWHAGLEPRIEGYEDHISVTSRAVACIEEHATDGAVVVFVGHGSTSRLAVRTL
                     IRLPLDGWNIGPLGNASWAELERASDGEWRLLGYNVGA"
     gene            2565..3638
                     /locus_tag="VF362_01860"
     CDS             2565..3638
                     /locus_tag="VF362_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7589704.1"
                     /translation="MNVRGWRTRGIQAALASALLFGAGTPLAKILLADVSPWMLAGLL
                     YAGSGIGLGLYRLARRAPRVRPRAGEWLPLAGAILCGGVAAPVLLFVGLSHLPASGAS
                     LLLNAESVFTALIAWWVFKENRGPRVVFGFAAIVAGAAVLAASSGVVVGGAWPSIAVL
                     AACLLWGVDNNLTRAVSRLDAVWLAALKGSVAGGVNLSLAFALGSRLPGAAQVLASMA
                     LGLAAYGVSLVLFIVALRKVGTARAGAYFAVAPFFGALVAFALGDAVTFPLAAAGLLM
                     ATGVWLHVTERHDHLHWHPPTAPYEAHTHDEFDERRDEAQGEARSDAADGAEEHRHAA
                     AHAALSHRHGHYPDTAHRHTHER"
     gene            complement(3687..4709)
                     /gene="gnd"
                     /locus_tag="VF362_01865"
     CDS             complement(3687..4709)
                     /gene="gnd"
                     /locus_tag="VF362_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX7589705.1"
                     /translation="MATDAPMQLGMIGLGRMGSNMVRRLMRDGHACVVYDLNPDSVAA
                     LVAKLDAPRSVWVMVPAGGITGRVIAELAALLEPGDAIIDGGNSYYRDDIDRSAELAA
                     KGITLLDCGTSGGVWGLDRGYCLMIGGDSPAVARLDPIFRTIAPGAGDVERTPGRTGP
                     TTSEELGYLHCGPSGAGHFVKMVHNGIEDGLMAAYAEGFNVLRSANIGRAAVSGCALS
                     GGAVSGDAETAPLANPEYYQFDIDTTAVAEVWRRGSVVGSWLLDLTATALLASRDLEE
                     FSGHVSDSGEGRWTSIAAIDEGVPTPVLTSALFTRFASRDANDFADKVLSAMRKGFGG
                     HEEKKT"
BASE COUNT          696 a         1617 c         1786 g          752 t
ORIGIN      
        1 gatatggcca gcctgtcagg ctcggacgtc gaggcgacgt ccctcgagga ggctcggtac
       61 accctgacct acgcctactc tggcggaggg gacgcggagt gcaaactgtg ggacgccgcc
      121 accaatgagc cggtctactt cctcaaaggg gcgaacttcg cccacgcctt ttccttggtg
      181 gatctttaca ccggcgcggt ggcggcgacg aaggtattcg gtagcacggc gatgaagtgc
      241 tgcacgagtg gtacgtacga cccgtccatg ttcgacggtc gtcaccaccg cgtcggagaa
      301 tgcgttgggg gcagcgagcc cgaatctacc tacagcgaga gtgaactcga ggcttggctc
      361 gagtcgcaga cggactaggg gagtgagcat gccaacggcg aagagtggcg gagaggccga
      421 gaagcccgac gcgaagggga gcgcggcggc acccaccgcg aagcgggaga cccccgcgat
      481 caggaagagc cgctccgccg gggacgaggg ggcctcgacg acgggcaaga acgctggtgc
      541 taagggcact cctgcgaaga cggcagacgg gggcgaggag gcctcgacca cgggcaagaa
      601 cgctggcgcc aagggcactc ccgcgaagac ggcagacgcg ggcgaggagg cgcccaagag
      661 gcggtggttc cggtctccca caatcctcgg tctcgcggga ggggtggtgc tggcggcgtg
      721 cctcaccgtc ttcgccctgc tggtgttcgg tgggcagcgt gacgtcgcga gaattgcggc
      781 gggcggattc cacacgtgcg cgatcgccac ctctggctcg gtcgagtgtt gggggtggaa
      841 cgagcacggc cagttaggga acggcacgac cgaatcgggc ggggaaccga ccgtcgtcga
      901 gggctcggcg ggagccatcg aggtcgcggc aggggccgcg catacgtgtg cgatcctcca
      961 agaccactcg ctctggtgct gggggtggaa cgtctcgggt cagttgggcg accgctcgac
     1021 tctcgaccga ccgactcccg tggaagtgtc cgggctcgcc gacgttcggc aggtctcggc
     1081 cgggactcac gcaacgtgtg ccgtgacatc ggacggatcg gcgtggtgtt ggggttggaa
     1141 ccagagcggg cagctcggcg acggcacgga agtggggcgc tccgggccgg tgctcgtcaa
     1201 ggatcttctg gacgtcgtcc aggtcagcgt gggagacgac cacgcttgtg cagtggtggg
     1261 ggacggcgag gtctggtgtt ggggatcgaa taccgcagga gaggttgggg cgacggccgc
     1321 gcagcaccag acgtcccccg tgaaggttga gggactcgcg ggcgccgtca aggtggcggt
     1381 cgggggagcg cactcgtgcg ccattctcaa cgacggttcc gtatcgtgct ggggggacaa
     1441 cgctttcggt caacttggcg ggccgtggta cacgaatgga tccgggggcc ctgtggcggt
     1501 cttggggctc gagggtgtca ccgccttggc gctcggctac gagcactcgt gtgccattct
     1561 cgacggcggt tccgtatcgt gctggggcgg taacgatcac ggtcaggtgg gcgacggaag
     1621 cgcgacggac cgaagtggtc cggttgacgt ggagggcatc gaggacgcca agcagatttc
     1681 cgcgggggac ttccacacgt gtgtccttcg gggcaacggc gaggtctcgt gttggggggc
     1741 cgacgccgac ggccagttgg gcgacggcgc cgcggaggac cgcgaccaac cggtcacggt
     1801 gatcggcctg agggccgggt agcgcccgag gggatcccgc gagcccgggg tccggtagcc
     1861 ttccggggtg acccgcgtct tccttcttcg ccacggcgag accgactgga acgccaccgg
     1921 ccgcctccag ggctccaccg acgtgcccat gaacgcgcgc gggctggagc aggtcggtgc
     1981 cgcggcccgt gcgctcggcg cgcgcttgcc gcagggaacc gtcgtcgtgt cgtcgccgct
     2041 ggtgcgcgcg cgcgccaccg ccgaggccct cgccgccctc accggcgcga cggtcgtgta
     2101 cgacgaccgc atgaaggaac gctcgtacgg cgcgtgggag gggatgaccg cggccgagcg
     2161 cgaggcgcac gactccgtcg aggtggcgcg ctggcacgcg gggcttgagc cccgcatcga
     2221 ggggtacgag gatcacatct ccgtgacctc ccgcgccgtg gcctgcatcg aggaacacgc
     2281 gaccgacgga gccgtggtcg tcttcgtcgg gcacgggagc acgtcgcgcc tggccgtcag
     2341 gaccctgatc cgcctccccc tcgacgggtg gaacatcggg ccgctgggca acgcctcgtg
     2401 ggccgaactt gagcgtgcct ccgacggcga gtggcgcctg ctcggctaca acgtcggcgc
     2461 atagccgctt cgcggcgggc ggttgcccgg cgacgcgctg tcgtccgatg gcccgccgca
     2521 ggcccgcgac gcgcgagtcg cgcggggtcc gccacactgg tgggatgaac gtccgcggct
     2581 ggcgcacgag ggggatacag gcggcactcg cctccgccct tctcttcggc gcgggtactc
     2641 cactcgccaa gatcctcctt gccgacgtga gcccgtggat gctcgctggc ctgctctacg
     2701 cgggctccgg aattgggctc gggctctatc ggctcgcccg gcgcgcgcca cgcgtgcgcc
     2761 ctcgcgctgg cgagtggttg ccgctcgctg gcgcgatcct ctgcgggggc gtcgcggccc
     2821 cggtgctcct cttcgtcggc ctttctcacc tcccggcctc gggcgcgtcg ctcctgctca
     2881 acgccgagag cgtgttcacc gccctgattg cgtggtgggt gttcaaggag aaccgcggac
     2941 cgcgcgtcgt gtttgggttc gccgccatcg tcgcgggcgc cgcggtgctc gccgcgtcgt
     3001 cgggagtcgt ggtcggcggc gcgtggcctt ccatcgccgt gcttgcggcg tgcctcctgt
     3061 ggggggtcga caacaacctg acccgcgcgg tctcacgcct cgacgcggtc tggcttgccg
     3121 cgctgaaggg ctcggtcgcg ggcggcgtga atctctcgct tgccttcgcg ctgggctcgc
     3181 gactgccggg ggctgcgcag gtactcgcgt cgatggcgct cggcctggcg gcgtacggcg
     3241 tgagcctggt gctcttcatc gttgcgctcc gaaaggtcgg gaccgcgcga gccggggcgt
     3301 acttcgcggt cgcgcccttc ttcggagccc tggtcgcctt cgcccttggg gacgccgtga
     3361 cgttccctct cgccgcggcc gggctcctga tggccaccgg agtctggctc cacgtgaccg
     3421 agcggcacga ccaccttcac tggcatcccc ccacggcgcc ctacgaggcc catacccacg
     3481 acgagttcga cgagcgcagg gacgaggcgc agggcgaggc gcgatcggac gcggccgacg
     3541 gggcggagga gcaccggcac gcggccgccc acgccgcgct cagccaccgc cacggccact
     3601 atcccgacac cgcgcaccgg catacgcacg agcgctagtt cccgggcgag cgtgatcgcg
     3661 cgaaacgggt ggaagcatgg agcgccctac gtcttcttct cctcgtgccc gccgaagccc
     3721 ttgcgcatgg cggagagcac cttgtcggca aagtcgttgg cgtcgcgcga ggcgaatcgc
     3781 gtgaagagcg cggaggtgag gacgggcgtc gggacgccct cgtcgatcgc ggcgatcgag
     3841 gtccagcgac cctccccgga gtccgagacg tgcccggaga actcctcgag gtctcgcgac
     3901 gcgagcaggg ccgtcgcggt caggtcgagg agccacgatc cgacgacgct cccgcgcctc
     3961 cagacctcgg cgacggcggt cgtgtcgatg tcgaactggt agtactcggg gttggcgagc
     4021 ggggcggtct ccgcatcacc cgacaccgcg ccgcccgaaa gcgcacagcc cgaaaccgcg
     4081 gcgcgcccga tgttggcgct tcgcaggacg ttgaagccct cggcgtacgc ggccatgagc
     4141 ccatcctcga tcccgttgtg caccatcttg acgaagtggc cggcgccgct cggcccgcag
     4201 tgcaggtacc cgagttcctc ggaggtggtc gggcccgtcc ggcccggggt ccgctccacg
     4261 tctcccgctc cgggagcgat cgtccggaag atcgggtcga ggcgcgcgac ggcgggggag
     4321 tcccctccga tcatgaggca gtagccgcga tcgaggcccc atacgccccc gctcgttccg
     4381 cagtccagca gggtgatgcc cttcgcggcg agctcggcgg agcggtcgat gtcgtcgcgg
     4441 tagtacgagt tgccgccgtc gatgatcgcg tctcccggct cgaggagcgc ggcgagttcg
     4501 gcgatcacgc gccccgtgat cccaccggcg gggaccatca cccagacgct tcgcggggcg
     4561 tcgagcttcg ctacgagagc cgcgaccgag tccgggttga ggtcgtagac gacgcacgcg
     4621 tggccgtccc gcatgagcct gcgcaccatg ttggagccca tgcgtccgag cccgatcatt
     4681 ccgagttgca tcggcgcgtc cgtcgccatt ccgcgtccct cctggagccc gatccgggcg
     4741 tcccacccgc ttagtgtgcg gggtgcgccc acccgccgca agcgcccacg cgccacgact
     4801 agtatctaga tagggctatg ttagcgccac tttggcggat aacataaaga a
//
LOCUS       DASVKP010000084         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_605369, whole genome shotgun sequence.
ACCESSION   DASVKP010000084 DASVKP010000000
VERSION     DASVKP010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605369"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..973
                     /gene="tsf"
                     /locus_tag="VF362_01870"
     CDS             134..973
                     /gene="tsf"
                     /locus_tag="VF362_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX7589706.1"
                     /translation="MANYTVADIQTLRDRTGAGMMDVKKALDEADGDLDKAVEILRVK
                     GLKGVTKREGRSASEGLVAAEVTDGASGQVGTMIEINSETDFVAKSPKFIDLATAVLA
                     AVVAADAETVDAALDADAGGKSVRVLIDETAGVLGEKIVLRRVARIEAPNVAVYLHKT
                     NRDLPAQVGVMVGSDDAAKDIARDVAMHIAAYSPFYLTREHVPADAIATERRVAEEVS
                     RAEGKPEAALPKIIEGKMNGYFKENVLVDQAFAKDPKLTVGAVVDQTGGAVTAFARFR
                     VGA"
     gene            1007..1756
                     /gene="pyrH"
                     /locus_tag="VF362_01875"
     CDS             1007..1756
                     /gene="pyrH"
                     /locus_tag="VF362_01875"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX7589707.1"
                     /translation="MPETSDRVAGIDIPPTARRVLLKVSGETFGAGVVGLDPDTVQRI
                     AREIAAAVAEHIQVAIVVGGGNFFRGVELSERGMERARADYMGMLGTVMNALALQDFL
                     EQAGVDTRVQTAITMGQVAEPYIPRRAMRHMEKGRVVIFAAGAGMPYFSTDTVAVQRA
                     LETRCSEVIFGKNGVDAVYTADPRTDPTAKRLERLTYGEALQRRLEVLDASAFALCSE
                     NSLPMVVFGLEQPGNVTRALLGERIGTLVTA"
     gene            1794..2351
                     /gene="frr"
                     /locus_tag="VF362_01880"
     CDS             1794..2351
                     /gene="frr"
                     /locus_tag="VF362_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX7589708.1"
                     /translation="MIDDTLLDVEDKMDKAVDVAKDEFSSIRTGQASTAMFAKITVDY
                     YGAPTPLQQLASISTPEPRTVLIAPFDKGAMKNIEAALRAGEMGFNPSNDGNVLRINM
                     PQLTEERRREYVKLARHKAEEARVTIRNLRRKAKDEIDALVKDGAVGEDEGARAEKEL
                     DSLTKRHVDAIDAVLAHKEDELLEV"
     gene            2351..>2555
                     /locus_tag="VF362_01885"
     CDS             2351..>2555
                     /locus_tag="VF362_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589709.1"
                     /translation="MSTWNPAELFGTREVTEERPGGRNLWLATLVGVVLFVGTVGAAR
                     YSELALTVFVYLLCIAAYPEWKRA"
BASE COUNT          476 a          831 c          888 g          360 t
ORIGIN      
        1 ccgtcgaggc gcccgtcgag gctgaggcgc ccgtcgtggc cgaggcgccc gctgcggcga
       61 agcctgccgc caagaagccc tcccccaaga agccctcccc caagaagacc gcagacaagg
      121 agtaaggatc gacatggcga actacaccgt tgccgacatc cagaccctcc gcgaccgtac
      181 gggcgcggga atgatggacg tgaagaaggc gctcgacgag gccgacggcg atctcgacaa
      241 ggccgtcgag atcctgcggg tgaagggcct caagggcgtc accaagcgag agggacgctc
      301 ggcgtccgag ggactcgtcg ccgcagaggt gaccgacggc gcctccggcc aggtcggcac
      361 catgatcgag atcaactcgg agacggactt cgtcgcgaag agccccaagt tcatcgatct
      421 cgcgaccgcc gtgctcgcgg ccgtcgtcgc cgccgacgcc gagaccgtgg atgccgcgct
      481 cgacgccgac gcgggcggca agagtgtgcg cgtgctcatc gacgagacgg cgggcgtgct
      541 tggagagaag atcgtgcttc gcagggtcgc ccgcatcgag gcgccgaacg tcgccgtcta
      601 ccttcacaag acgaaccgcg accttcccgc ccaggtgggc gtcatggtcg gctcggacga
      661 cgcggcgaag gacatcgccc gcgacgtggc gatgcacatc gcggcgtact cgcccttcta
      721 cctgacgcgc gagcacgtgc ccgccgacgc gatcgcgacg gagcggcgcg tcgccgaaga
      781 ggtctccagg gcggaaggca agcccgaggc cgcgctgccc aagatcatcg aggggaagat
      841 gaacggctac ttcaaggaga acgtgcttgt cgaccaggcg ttcgccaagg accccaaact
      901 gaccgtgggc gccgtcgtgg atcagacggg cggcgcggtt accgcgttcg ctcgcttccg
      961 cgtgggagcc tagagaccag cgaggaccgc gtgcggagga ggggcgatgc ccgagacgag
     1021 tgacagggtc gcgggcatcg acatccctcc gaccgcgcgg cgagtccttc tcaaggtctc
     1081 cggagagacg ttcggcgcgg gggtggtcgg gctcgacccc gacaccgtcc agcggatcgc
     1141 gcgagagatc gcggccgccg tcgccgagca catccaggtg gcgattgtcg tcgggggcgg
     1201 gaacttcttc cgcggcgtcg aactgtccga acgcggcatg gagcgcgcgc gcgcggacta
     1261 catgggcatg ctcggcaccg tgatgaatgc gctcgccctt caggacttcc ttgagcaggc
     1321 gggcgtagac actcgcgttc agaccgcgat caccatgggt caggtcgccg agccgtacat
     1381 tccccgcagg gcgatgcgcc acatggagaa gggtcgtgtg gtgatcttcg ccgcgggggc
     1441 gggaatgccc tacttctcca ccgatacggt cgcggtccag cgggccctcg agacgcggtg
     1501 ctccgaggtg atcttcggca agaacggcgt cgacgccgtc tacacggcgg acccgcggac
     1561 cgatccgacc gcgaagcgcc tggagcgcct gacctatgga gaggcgcttc agcggcggct
     1621 cgaggtgctc gacgcctctg ccttcgccct gtgcagcgag aacagtctcc cgatggtcgt
     1681 cttcggcctc gaacagccgg gaaacgtgac ccgcgccctt ctcggtgaga gaatcgggac
     1741 gctggttacc gcgtgacgcc gcttgacgtg aatacgcaac gcaaggagcc tctgtgattg
     1801 acgacaccct cctcgatgtc gaggacaaga tggacaaggc cgtcgacgtg gcgaaggacg
     1861 agttctcctc catccgcacg ggacaggcct ccacggccat gtttgcgaag atcaccgtgg
     1921 actactacgg cgccccgacg ccgctgcaac aactcgcctc catcagcacg ccggagccgc
     1981 ggacggtcct catcgcgccc ttcgacaagg gcgccatgaa gaacatcgag gccgcgctgc
     2041 gcgccggcga gatggggttc aacccgagca acgacggaaa cgtcctcagg atcaacatgc
     2101 ctcaactgac cgaggagcgc cggcgagagt acgtcaagct cgcacggcac aaggcggagg
     2161 aggcgcgggt gacgatccgc aaccttcgcc gcaaggcgaa ggacgagatc gacgcactgg
     2221 tgaaggatgg cgcggttggc gaggacgagg gcgcgcgcgc cgagaaggaa ctcgactccc
     2281 tcaccaagag gcatgtggac gccatcgacg ccgtcctggc gcacaaggaa gacgagttgc
     2341 tcgaggtcta atgtcgacgt ggaatccggc cgaactcttc ggcacgcgag aggtgaccga
     2401 ggagcgacca ggaggccgca acctctggct cgcgaccctc gtgggcgtgg tcctgttcgt
     2461 ggggaccgtg ggtgccgcgc gatacagcga gttggccctg acggtgttcg tctacctgct
     2521 gtgcatcgcg gcgtatccgg agtggaagcg cgcgc
//
LOCUS       DASVKP010000085         1857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049 k141_27473,
            whole genome shotgun sequence.
ACCESSION   DASVKP010000085 DASVKP010000000
VERSION     DASVKP010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27473"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /gene="rph"
                     /locus_tag="VF362_01890"
     CDS             complement(<1..523)
                     /gene="rph"
                     /locus_tag="VF362_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX7589710.1"
                     /translation="MTDTIRADGRTPSQLRPVSFERGWQASAEGSCLVTFGGTRVLCS
                     ASFTQGVPRWKQNSGQGWVTAEYAMLPRATGTRNDRESVKGRIGGRTHEISRLIGRSL
                     RAIIDVKALGENTIVLDCDVLQADGGTRTAAITGAYLALADAIAWGKVHGAIAPGAKA
                     LIDSVSAVSVGIIG"
     gene            complement(590..1396)
                     /gene="murI"
                     /locus_tag="VF362_01895"
     CDS             complement(590..1396)
                     /gene="murI"
                     /locus_tag="VF362_01895"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX7589711.1"
                     /translation="MNDAPIGVFDSGVGGLTVARAIMDVLPNEAIHYVGDTAHGPYGP
                     LPIAEVRSRALAIMDSLVDDGVKMLVIACNTASAAVLRDARERFSKEKGVPVVEVIVP
                     AVRRAVTTTRTGRIGVIGTVATITSGAYEDALAAAAGIAVTSQACPRFVEFVEAGVTS
                     GPELTELAREYLAPLREAGVDTVILGCTHYPMLEGVIGYVMGEDVALIDSATETALDV
                     YRGLVAGGIERTSSELPEHRFSATGETARFRALAQRFLGPVVGAVEGIAR"
     gene            complement(1389..>1857)
                     /locus_tag="VF362_01900"
     CDS             complement(1389..>1857)
                     /locus_tag="VF362_01900"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HEX7589712.1"
                     /translation="DLLDYLTNLEAHLAEPDDPAILRLLPNAAPTDREVADEFRRYTE
                     PDLRALKVARLRRLWSQLSEDGPRWEVAEDEAMETAAALTDVRLVLASRLGIETDADA
                     ERLHADVGVAQQAAVGGIVQDRERIWLGMLYEALTWLQDSLVAFVMGEEGDDE"
BASE COUNT          271 a          673 c          639 g          274 t
ORIGIN      
        1 cgccgatgat gccgacgctc accgcgctga cggagtcgat gagcgccttc gcgcccggag
       61 cgatcgcccc gtgaaccttg ccccatgcga tggcgtcggc cagggcgagg tatgcccccg
      121 tgatcgccgc cgtccgcgtg ccgccatccg cctggagcac gtcacagtcg aggacaatcg
      181 tgttctcccc gagggccttg acgtcgatga tggcgcgcag cgaccggccg atgaggcgcg
      241 agatctcgtg ggtgcgcccg ccgatgcggc ccttgacgct ctcgcgatcg ttgcgcgtgc
      301 cggtggccct gggcaacatg gcgtactcgg cggtcaccca accctggccc gagttctgct
      361 tccagcgcgg gacgccctgg gtgaaggagg ccgagcacag gacgcgggta ccgccgaagg
      421 tgacgaggca cgagccctcg gcgctcgcct gccacccgcg ctcgaaggag acggggcgga
      481 gctgggaggg agtgcggcca tcggcgcgga tcgtatcggt catgccccga gcctaaccgg
      541 gagccgcgac cccgcgggcg cgccccagca tttcctactg cacggtcccc tatcgcgcga
      601 tgccctcgac cgcgccgacg acgggcccga ggaagcgctg ggcgagggcc cggaatcgcg
      661 cggtctcccc cgtcgcgctg aaccgatgct cgggaagttc ggaactcgtt ctctctatcc
      721 cgcctgccac gaggccccgg tacacgtcga gggccgtctc ggtcgcgctg tcgatcagcg
      781 cgacgtcctc ccccatgacg tatccgatca cgccctcgag catggggtag tgggtgcagc
      841 ccaagatgac ggtgtcgacg cccgcctcgc gtagcggcgc gaggtactcg cgggcgagtt
      901 ccgtcagttc ggggccgctc gtgacgccgg cctcgacgaa ctccacgaag cgcgggcacg
      961 cctgggacgt gaccgcgatc cccgccgcgg cggcgagggc gtcctcgtag gcgcccgacg
     1021 tgatcgtcgc gaccgtgccg atgacgccga tcctgcccgt ccgcgttgtg gtgacggccc
     1081 ggcgcacggc ggggacgatc acctcgacga cgggcacgcc cttctccttg ctgaagcgct
     1141 cgcgcgcgtc cctcaggacc gcggcggacg ccgtgttgca ggcgatgacg agcatcttga
     1201 ccccgtcgtc gacgagggag tccatgatcg cgagcgcgcg ggacctcacc tccgcgatgg
     1261 gaaggggacc gtacggcccg tgggcggtgt cgcccacgta gtggatggcc tcgttcggca
     1321 ggacgtccat gatcgccctg gccacggtga gccccccgac cccagagtca aagactccaa
     1381 tcggcgcgtc attcatcgtc accctcctcg cccatgacga acgctaccaa ggagtcctgg
     1441 agccaggtga gggcctcgta caacatgccc agccagatcc gctcccgatc ctggacgatc
     1501 ccgccgacgg cggcctgctg cgcgacccca acgtccgcgt ggagccgctc ggcatccgcg
     1561 tccgtctcga tccccagtcg cgacgccagc accaggcgga cgtccgtcag agccgcagcc
     1621 gtctccatgg cctcgtcctc ggcgacctcc caccgcgggc cgtcctcgct cagttgcgac
     1681 cagaggcggc gaagcctcgc gaccttcagc gcgcgcaggt ccggctcggt gtagcgacga
     1741 aactcgtcgg cgacctcgcg atccgtcggg gcggcgttgg gcaggagccg caggatcgcg
     1801 gggtcgtcgg gctccgccag gtgggcctca aggttggtga ggtagtcgag gagatcc
//
LOCUS       DASVKP010000086         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_522429, whole genome shotgun sequence.
ACCESSION   DASVKP010000086 DASVKP010000000
VERSION     DASVKP010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522429"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VF362_01905"
     CDS             <1..214
                     /locus_tag="VF362_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7589713.1"
                     /translation="MSSVVASFVGVAPADQPRIVVSVMLYNPKTSQWGGTVAGPIFSD
                     VMGMALQSLGIPPSGTAAQLYPTTWG"
     gene            226..1758
                     /locus_tag="VF362_01910"
     CDS             226..1758
                     /locus_tag="VF362_01910"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX7589714.1"
                     /translation="MAVRPTHVDPVPASKVASRAGARLDPPDANVGLTGATVDSRGVL
                     PGDLFGAIPGFSTHGATFAAAAVAAGAAAVLTDTQGAALASPTGVPVIVSDDPRRALG
                     LASAAIYGDPSRALTLIGVTGTNGKTTTAYFIDAALRRVHAKTGLLGTVEMRIGGESL
                     PAVRTTIEAPAFQAILARMLEVGVGAATMEVSSHALELGRVAGSHFAVAAFSNLQWDH
                     LDFHATMEDYFRAKARLFTPELAERGVVCVDDEWGVRLAKEATVPVVRVATRPEAGAA
                     EWTVADTRLAADGVGTDFALVGPAGERIEASSPLPAHINVSNAALAIIVAIEAGVPVA
                     DAVAGVASTPGVPGRMQRVLERDAETPLAIVDYAHTPDALERALEGARTVTPGRLISV
                     FGAGGDRDTGKRPQFGKVAGALADVVIVTDDNPRSEEPGAIREGILAGLRRERPSMRD
                     VHEVAPREEAIRFALALAAPGDTILLSGKGHEDYQEVAGVKHHFSDQEELLKALAESG
                     RS"
BASE COUNT          267 a          676 c          679 g          261 t
ORIGIN      
        1 gatgtccagc gtcgtcgcct cgttcgtggg ggtagcgccg gccgaccagc cgcgtatcgt
       61 ggtctccgtg atgctctaca acccgaagac gtcgcagtgg ggcggaacgg tcgcaggccc
      121 catcttctcg gatgtcatgg gcatggcgct gcaatccctc gggattccgc caagcgggac
      181 cgcggcccag ttgtacccga cgacgtgggg gtgagcgtgg agggtatggc ggtgcgtccg
      241 actcacgtcg accccgtgcc cgcttccaag gtcgcctccc gggcgggcgc gcgcctcgac
      301 ccgccggacg cgaatgtggg cctcaccgga gcgaccgtcg atagcagggg agtcctaccc
      361 ggcgatctct tcggcgccat accaggattc tcgacgcacg gcgcaacctt cgccgccgcg
      421 gcggtggcag ccggggcggc cgcggtgctc accgacaccc agggcgccgc gctcgccagc
      481 ccgaccggcg tccccgtgat cgtctccgac gatcctcggc gcgcgcttgg gctcgcgtcc
      541 gccgcgatct acggcgaccc ctcgcgggcg ctgaccctga tcggggtcac cgggacgaac
      601 ggcaagacca cgaccgcgta cttcatcgac gccgcgctgc gcagggtcca cgcgaagacg
      661 gggctgctgg ggaccgtgga gatgcggatc ggcggagagt cgctgcccgc cgtgcggacc
      721 acaatcgagg cccccgcctt ccaggcgatt ctcgcgagga tgctcgaggt cggcgtgggc
      781 gccgccacga tggaggtctc gtcccacgcg ctcgaactcg gccgcgtggc gggctcccac
      841 tttgcggtcg cggccttctc caacctccag tgggaccacc tggactttca cgcgaccatg
      901 gaggactact tccgcgcgaa ggcgcgcctc ttcacgcccg aattggccga gcgtggggtc
      961 gtctgcgtgg acgacgagtg gggggtgcgg ctcgcgaagg aggccacggt ccccgttgtc
     1021 cgcgtcgcaa cgagacccga ggcgggcgcg gcggaatgga cggtcgcgga cacgcgcctc
     1081 gccgccgacg gcgtcgggac ggacttcgct ctcgtggggc ccgcagggga gcggatagag
     1141 gcgtcgagcc ccttgcccgc ccacatcaac gtctccaacg cggcgctggc gatcatcgtc
     1201 gcgatcgagg cgggagtccc cgtcgcggac gccgtcgccg gggtagcgag cacgcccggc
     1261 gttccaggcc gaatgcagcg cgtgctcgag cgggacgccg agacgccgct ggcgatcgtg
     1321 gactacgccc acacgccgga tgcgctcgag cgcgcgctcg agggcgcccg caccgtgacc
     1381 cccgggcgcc tgatctccgt cttcggcgcg ggcggcgacc gcgacacggg caagcgcccc
     1441 caattcggca aggtcgccgg agcgctcgcc gacgtcgtca tcgtcaccga cgacaacccc
     1501 cgttcggagg agccgggcgc gatccgggag gggattctcg cggggctccg tcgggagcgg
     1561 ccctccatga gggatgttca cgaggtcgcc ccgcgcgagg aggcaattcg cttcgcgctc
     1621 gcgctcgccg cgcccggcga cacgatcctc ctgagcggca agggccacga ggactaccag
     1681 gaggtcgcgg gggtcaagca ccacttcagc gaccaggagg agctgctgaa ggcgctcgcc
     1741 gaatcgggga gatcctgatg cgccccgtca ccgcccggtg gatcgcggag gtcacggggg
     1801 gcgcgctcgc cgcgggaagc gacaccgagg tcacctccgt gtcgcaggac tcccgctcct
     1861 gccagccggg ctcgctctac gtc
//
LOCUS       DASVKP010000087         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_358753, whole genome shotgun sequence.
ACCESSION   DASVKP010000087 DASVKP010000000
VERSION     DASVKP010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358753"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /gene="sufC"
                     /locus_tag="VF362_01915"
     CDS             <1..729
                     /gene="sufC"
                     /locus_tag="VF362_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX7589715.1"
                     /translation="KLSVDTKEGTKPILRGVDLTIRSGETHAIMGPNGSGKSTLAYSL
                     AGHPKYKVTDGSVTLDGRDVLAMKVDERARAGLFLAMQYPVEVPGVSVSNFLRTAKTA
                     VAGEAPKLRTWIKEVKEAMDNLKMDPAFAERNVNEGFSGGEKKRHEILQMELLAPKFA
                     ILDETDSGLDIDALRVVSEGVNRAKAATGAGVLLITHYTRILRYIDADFVHVFVDGQI
                     AEQGGPELADSLEAEGYDRFLAKA"
     gene            741..>1575
                     /locus_tag="VF362_01920"
     CDS             741..>1575
                     /locus_tag="VF362_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7589716.1"
                     /translation="MFPDLRSDFPALGREGRNGRLLVYLDSAATSHKPGQVLQAEADF
                     YRFSNGAVGRGAHLLAEEATEAFEGARAAVAAFVGAGPEQIVWAENATAALNLVASGI
                     GNASQGVGGDAGKRFAIGEGDEIVVTESEHHANLIPWQVLARRTGATLRWIAVDDAGR
                     LDLGSLADVVGERTRVVAFQHASNVTGIIHPVEAIIARAREVGALTVMDACQTAPHLP
                     LDLTALGVDFAAFSGHKMLGPTGIGALYGRTELLNALPPSVFGGSTVKSVTMTDTTWL
                     DA"
BASE COUNT          257 a          577 c          518 g          223 t
ORIGIN      
        1 aagctcagcg tcgacaccaa ggaaggcacc aagccgatcc ttcgcggcgt cgacctcacc
       61 atcaggtccg gcgagaccca cgccatcatg ggcccgaacg gctcgggcaa gtccacgctc
      121 gcgtactcgc tcgcgggcca cccgaagtac aaggtcaccg acggctccgt cacgctcgac
      181 ggtcgggacg tcctcgcgat gaaggtggac gagcgcgccc gcgcgggcct gttcctcgcc
      241 atgcagtatc ccgtcgaggt ccccggcgtt tccgtctcga acttcctccg cacggccaag
      301 acggccgtcg cgggcgaggc ccccaagctc cgcacgtgga tcaaggaggt caaggaggcg
      361 atggacaacc tcaagatgga tccggccttc gccgagcgca acgtcaacga gggcttcagc
      421 ggcggcgaaa agaagcgcca cgagatcctt cagatggaac tcctcgcccc caagttcgcg
      481 atcctcgacg agacggactc cggcctcgac atcgacgccc tccgcgtggt gtccgagggc
      541 gtgaaccgcg ccaaggccgc gacgggagcc ggcgtcctgc tcatcacgca ctacacgcgc
      601 atccttcgat acatcgacgc cgacttcgtc cacgtgttcg tggacggcca gatcgccgag
      661 cagggcgggc cagagctcgc cgacagcctc gaggccgagg gctacgaccg cttcctcgcg
      721 aaggcgtagc cggtcccgtc atgtttcccg atctccgctc cgacttcccc gccctcgggc
      781 gcgaggggag gaacgggcga ctcctcgtgt acctcgactc ggccgcgacc tcgcacaagc
      841 ccggccaggt tctccaggcc gaggcggact tctatcggtt cagcaacggg gcggtcggtc
      901 gcggggcgca cctgcttgcc gaagaggcga cggaggcctt cgagggcgcc cgcgccgcgg
      961 tggctgcctt cgtcggcgcc ggtcccgagc agatcgtgtg ggcggagaac gcgaccgcgg
     1021 cgctcaacct cgtggcctcg ggaatcggca acgcctccca gggcgtcggg ggcgacgccg
     1081 gcaagcgctt cgccatcggc gagggcgacg agatcgtcgt cacggaatcc gaacaccacg
     1141 cgaacctcat cccgtggcag gtcctggcgc gccgcacggg cgcgacgctc cgctggatcg
     1201 ccgtcgacga cgcgggccga ctcgacctcg ggagtctcgc cgacgtcgta ggggagcgga
     1261 cgcgcgtcgt cgccttccag cacgcgagca acgtcacggg catcattcac cccgtcgagg
     1321 cgatcatcgc gcgggcccgc gaggtgggcg cgctcacggt gatggacgcg tgccagaccg
     1381 cgccgcacct gccgcttgac ctcaccgccc tcggcgtcga ctttgccgcc ttctcgggtc
     1441 acaagatgct cgggcccacc ggcatcgggg ccctctacgg ccgcaccgaa ctgctcaacg
     1501 cccttccgcc gtccgtgttc ggcggctcga cggtcaagag tgtgacgatg acggacacga
     1561 cctggctcga cgcgc
//
LOCUS       DASVKP010000088         6056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_359712, whole genome shotgun sequence.
ACCESSION   DASVKP010000088 DASVKP010000000
VERSION     DASVKP010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359712"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..296)
                     /locus_tag="VF362_01925"
     tRNA            complement(223..296)
                     /locus_tag="VF362_01925"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Pro,seq:cgg)
     gene            complement(391..1308)
                     /locus_tag="VF362_01930"
     CDS             complement(391..1308)
                     /locus_tag="VF362_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7589717.1"
                     /translation="MTLLGSAALGVAGIAAGGLAWGAVEARAFTTRHVRVPALAEGSG
                     PIRVLHISDIHLMPSQRAKLRWLGTLADLKPDLVVNTGDNLAHPRAAGPLLDALGPLL
                     ALPGAFVHGSNDYFRAVPKNPFTYFRGPTSLRTGPAALPWEALTDAFVAAGWRDLRNA
                     RATMEVGGARVDLVGLDDPHIDRDRMPAASPGAGGARLRLGLVHAPYARALAALRDDG
                     ARVILAGHTHGGQVCIPGLGALVTNCDLDRRRARGLSGWPGARPDRPGGEGSVWLNVS
                     AGIGTSPYARVRFACRPEASLLTLVAAGT"
     gene            complement(1325..3769)
                     /locus_tag="VF362_01935"
     CDS             complement(1325..3769)
                     /locus_tag="VF362_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX7589718.1"
                     /translation="MSRLRSSRAGRLTLMELLGSFVAFLMLSVVAGVLLSGFAIPAAT
                     IAGSAAKGGIGLFDEMPADLQFGDLPQQSNIYAADGTLIAQFYDQNRVVVPLQDISPW
                     LQQATVAVEDKRFWQHNGVDGEGLLRAVYINLTSSNSPGASTLTQQLVKNTLVQAAVA
                     SGDTKAIKDATEVSVARKLREARYALNLEKYFGDVYGRVCSADPKVDCGKESILERYL
                     NIAQFGGSVYGVETASQLYFGHSAKEDTALEAATIVGITKNPFAYDPLLKPKASQDRR
                     DTVLLTMRQQGMISQQEYDEYVATPIADTLHINKPKMSCSAAVDAPFFCDYVTKILAN
                     DPVFAGQGMTWLKQGVNIYTTLDVTKQQIANNVLRSTIPQGDASGFADAMVSLDPSTG
                     AILVMAQDTGYDPSATPDPGMTSINYSTDREYGGSRGFSPGSTFKPIVLATWLADGHG
                     LDEVIGADHRKWDPASWKASCLGPHPFAGSKPWDPANSDGQGKGQMTALSATQGSVNT
                     AFAAMTNRLDLCHIRDMALKLGFKRADGADFEIVPSASLGTQNASPLTMASVAQVFAN
                     DGLKCDPSAILRVETIDGKYLGGQASTCKQVITQAVARGVKYGMQQVMLGGTGSSSRL
                     AGGRPSAGKTGTSQENMHGWFMGFTAQLVTVTWEGNPTQDVPQHNIWIGGVHYRSVFG
                     ATISGQNWKHFMDAALAGQPNIALPGRPAGLPSSWKGTVPDVRGKTALDAEHAINDEG
                     FTWSINPVPTYSPIVPEGSVVSQWPAAGSPASSSATVEITLATSTPPPPPPPAPDPKP
                     SPKPSPKPSPTPAPTP"
     gene            3842..3997
                     /locus_tag="VF362_01940"
     CDS             3842..3997
                     /locus_tag="VF362_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007926759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEX7589719.1"
                     /translation="MTTWEYATVPLIDHATKQILDQWGRDGWELVQVLVMPSGGLVAY
                     LKRPLAA"
     gene            3999..4466
                     /locus_tag="VF362_01945"
     CDS             3999..4466
                     /locus_tag="VF362_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7589720.1"
                     /translation="MTASQRLAELGIVLPVVATPVGSYVPAVAYGTVVRTSGQLPLEG
                     GALRAKGLVGEGPGCVSLEDAAACARIAALGALAALAEVAGGVDAIGRIVRVVGYVAS
                     EPGFTGQAAVLNGASALMTEVFGEAGRHVRSAVGVVSLPLGAPVEIEVEALLA"
     gene            complement(4463..5158)
                     /locus_tag="VF362_01950"
     CDS             complement(4463..5158)
                     /locus_tag="VF362_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7589721.1"
                     /translation="MAQDRAQEENALRSSLLLSAMDPETARSLISVMTRREFSRGAVM
                     FFEGAPGDALYVIVKGKVKLARTARDGRENLLSLLGVGDMVGELSVFDPGPRTSRAHV
                     PEETVAYELTKQALDAWLKDHMEASRYLLRALAKRIRRTSITMADLVFTDVPGRVAKA
                     ILDLSHRFGGADRGQVIVRHGLTQEELAQLVGASRETVNKALADFASRGWIDVHMGSV
                     VVYEPDRLRARAR"
     gene            complement(5172..5543)
                     /locus_tag="VF362_01955"
     CDS             complement(5172..5543)
                     /locus_tag="VF362_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589722.1"
                     /translation="MAHAVAQTVADLRQQRRELRRELARVRWWRRLIASRRELSIAQM
                     AGATDPDANVEAAWEALASGAPTSSELAEVIWPGAESATADLVQRLEEIDGRLDSYER
                     RLSANLEGVTADMIDAMGAAR"
     gene            5638..>6056
                     /locus_tag="VF362_01960"
     CDS             5638..>6056
                     /locus_tag="VF362_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX7589723.1"
                     /translation="MASRPPLTVAIAGDEPPDAALVRRARRVNRILAGEYPDARCELD
                     YQNPFELIVATVLSAQCTDVQVNLVTPALFAAFPDAEALAAAPRERLEALIRSTGFFH
                     AKAQSLLELSRALVERFGAEVPPHLDDLVTLRGVGRKT"
BASE COUNT          932 a         2062 c         2089 g          973 t
ORIGIN      
        1 ctgaatgcgg ttcagatagc gcaactgctc gtgagccaca tcaagaagta ggggccagtt
       61 ggtcggggtg tccggcgcct tcggcggcta caaacaccga acgcagaggc gggacggcaa
      121 cgccgtcccg ctatctcagt ccgggtgttc gccgcttcgc ggcttcgacc ccttcctcgt
      181 tcgcgtgaag cgagagcaag ctctcgcttc acgctctctc agtcggggtg acaggatttg
      241 aacctgcggc ctcttcgtcc cgaacgaagc gcgctaccaa actgcgccac accccgtgaa
      301 ccccgtcgag cggatgcgcg accgagccag cctaggatag ccgaccgcgg ggcggcgcac
      361 gaaacgacaa gcccgcgaag ccccgctcgc ctacgtgcct gcggctacca gggtgaggag
      421 tgacgcctcg gggcggcacg caaagcgcac ccgcgcgtat ggcgacgtcc cgattcccgc
      481 ggagacgttg agccagaccg agccttcgcc acccggccgg tccggccgcg cgccgggcca
      541 accgctcagc cctcgggcgc ggcggcggtc gaggtcgcag ttcgtcacga gtgctccgag
      601 gccggggatg cacacctggc ccccgtgggt gtgccccgcc aggatgaccc tcgctccgtc
      661 gtcgcggagc gcggcgaggg ccctcgcgta cggcgcgtgg accagcccca ggcgcaggcg
      721 cgcgccgcca gctcccggcg aggccgcggg catccggtcg cggtcgatgt gcgggtcgtc
      781 gaggccgaca aggtcaaccc tcgccccgcc gacctccatc gtcgccctcg cgttcctcag
      841 gtcccgccat cccgcggcga cgaaggcgtc cgtcagggcc tcccaaggca acgcggccgg
      901 gccggtccga agcgacgtgg ggccgcgaaa gtaggtgaag ggattcttgg ggaccgcgcg
      961 gaagtagtcg ttcgacccat gaacgaacgc gccgggaagg gccagcagtg ggcccagggc
     1021 atcaagaagc ggccccgccg ctcggggatg cgccaggttg tcgcccgtgt tgacgaccag
     1081 gtccggcttc aggtcggcca gcgtgccaag ccagcgaagc ttggcccgct ggctcggcat
     1141 caggtgaatg tccgatatgt ggaggactcg gatcggcccg gagccctcgg ctagcgcggg
     1201 cacgcggaca tgccgcgtcg tgaacgccct ggcctcgacg gccccccacg ccaatccgcc
     1261 cgccgcgatc cccgcgacgc cgagcgccgc ggaaccgagg agcgtcacgc ggcccgggcc
     1321 ccactcacgg cgtcggggcc ggcgtcgggg acggcttagg ggacggctta ggggacggct
     1381 tagggtccgg cgcggggggc ggcggcggcg ggggcgtcga ggtggcgagg gtgatctcga
     1441 ccgtcgcact cgaactcgcc ggcgagcccg ccgctggcca ctgagagacg accgagccct
     1501 cgggcacgat gggcgagtag gtcgggaccg ggttgatcga ccacgtgaag ccctcgtcgt
     1561 tgatcgcgtg ctcggcgtca agggcggttt tgccccgaac gtcggggacg gtgcccttcc
     1621 acgacgatgg aagcccggct ggcctgccgg gaagcgcaat gttcggctgg cccgcgagag
     1681 ccgcgtccat gaagtgcttc cagttctggc cggagatcgt ggcgccgaag acgctcctgt
     1741 agtgaacgcc gccgatccag atgttgtgct ggggaacgtc ctgggtcggg ttgccctccc
     1801 acgtgaccgt cacgagttgc gccgtgaagc ccatgaacca tccgtgcatg ttctcctggg
     1861 aggttcccgt cttgcctgcc gaggggcgcc cgcctgcgag cctcgaggac gagcccgttc
     1921 cgccgagcat gacctgctgc atgccgtact tgaccccgcg ggcgaccgcc tgggtgatga
     1981 cctgcttgca cgtgctggcc tggcccccga ggtacttccc gtcgatcgtc tccacgcgga
     2041 ggatcgcgct cgggtcgcat ttgaggccgt cgttcgcgaa gacctgggcg accgacgcca
     2101 tcgtgagggg cgaggcattc tgcgttccaa ggctcgcgga ggggacaatc tcgaagtccg
     2161 cgccgtccgc ccgcttgaac ccaagcttga gggccatgtc gcggatgtgg cacaggtcga
     2221 ggcggttggt catggcggca aacgccgtgt tgaccgaacc ctgggtagcg gaaagcgccg
     2281 tcatctggcc ttttccctgg ccgtcggagt tcgccggatc ccagggcttc gaaccggcga
     2341 aggggtgcgg cccgaggcac gaggccttcc aggacgccgg gtcccatttc cggtggtcgg
     2401 cgccaatgac ctcgtccagg ccgtggccgt ccgcgagcca ggtggcaagg acgatcggct
     2461 tgaaggtgga accgggggag aatccgcggg agccgccgta ctcgcgatcg gtcgagtagt
     2521 tgatggacgt catgcccgga tccggcgtcg cggacgggtc gtaccccgtg tcttgggcca
     2581 tcaccagaat cgcgccggtc gacggatcga gggacaccat ggcgtccgcg aaccccgacg
     2641 catccccctg agggatcgtg cttcggagca cgttgttcgc gatctgttgc ttggtgacgt
     2701 cgagggtcgt gtagatgttc acgccctgct tgagccacgt catgccctgg ccggcgaaga
     2761 ccgggtcgtt ggcgaggatc ttcgtcacgt agtcgcagaa gaagggagca tcgacggcgg
     2821 cgctacaact catcttgggc ttgttgatgt gcagcgtgtc ggcgatcggc gtggcgacgt
     2881 actcgtcgta ctcctgctgc gagatcatgc cctgctgccg catggtgagg agcaccgtgt
     2941 cgcgccggtc ctgggaggcc ttcggcttga ggagggggtc gtaggcgaac gggttcttgg
     3001 tgattcccac gatcgtcgcg gcctcgagcg ccgtgtcctc cttggcggag tgcccaaagt
     3061 acaactgcga ggcggtctcg actccataca cggacccgcc gaactgtgcg atgttcaggt
     3121 agcgctcgag gatgctctcc ttgccgcagt cgaccttggg atccgcgctg cacacgcggc
     3181 cgtagacgtc gccgaagtac ttctcgaggt tgagcgcgta gcgcgcctcc cgaagcttcc
     3241 tcgcgaccga gacctcggtg gcatccttga tcgccttggt gtcgcccgac gcgacggccg
     3301 cctgcaccag ggtgttcttc acaagttgct gggtgagcgt cgacgcaccg gggctatttg
     3361 atgaggtgag gttgatgtag acggcgcgaa gcaggccctc gccgtcgacg ccgttgtgtt
     3421 gccagaagcg cttgtcctcg acagcgacgg tcgcctgttg gagccacggc gagatgtcct
     3481 gaaggggcac gaccacgcgg ttctggtcgt agaactgggc gatcagggtg ccgtcggccg
     3541 cgtagatgtt ggactgctgc ggcaggtccc caaactgaag atccgccggc atctcatcga
     3601 agaggccgat cccgcccttg gccgctgaac cggcgatggt cgcggcgggg atggcgaagc
     3661 ctgagaggag taccccggcc acgacggaca gcatgaggaa ggcgacgaac gagcccagaa
     3721 gttccatcag ggtcaaccgt ccggcgcgcg aagaacggag tcgcgacatg gcaccacctt
     3781 acgtcggatg cccaacgaat gaggactctc ccgggttgca cttcgggggt accctcgcga
     3841 tatgaccacc tgggagtacg ccaccgtgcc gctcatcgac cacgccacca agcagatcct
     3901 cgatcaatgg gggcgggacg gatgggaact cgtccaagtt cttgtcatgc cgagcggcgg
     3961 cctcgtcgcg tacctgaagc gacccctggc ggcctagcgt gacggcatcg cagaggctcg
     4021 cggaactcgg catcgtgctc ccggtcgtcg ccacccccgt gggctcctat gtccccgccg
     4081 tcgcctatgg aacggtggtc cgcacatcgg ggcagcttcc gctcgagggc ggggcgttgc
     4141 gggcgaaggg actcgtgggc gaggggcccg ggtgcgtctc gctcgaagac gccgccgcat
     4201 gcgcgaggat cgccgcgctc ggcgcgctcg ccgcccttgc cgaggtcgct ggcggcgtcg
     4261 acgcgattgg tcgcatcgtc cgcgtggtcg gctacgtggc ctcggagccc ggcttcactg
     4321 ggcaggcagc cgtgctgaac ggcgcgagcg cgctgatgac cgaggtcttc ggcgaggcgg
     4381 gaaggcacgt gaggtcggcg gtcggcgtgg tcagtctccc gctcggggcc ccggtcgaga
     4441 tcgaggtgga ggcgctcctc gcctagcggg cgcgagcgcg aagccggtcg ggctcgtaga
     4501 cgacgaccga gcccatatgc acgtcgatcc agccgcggct cgcgaagtcc gccagggcct
     4561 tgttgaccgt ctcccgcgac gcgccgacga gttgagccaa ctcctcctgg gtgagtccat
     4621 ggcgaacgat cacctgtccg cgatcggctc cgccgaatcg gtggctgagg tcgaggatcg
     4681 ccttcgccac tcgcccggga acgtcggtga acacgaggtc ggccatcgtg atgctcgtgc
     4741 gccgaatgcg cttcgcgagc gcccgcagca ggtaccgcga ggcctccatg tggtccttga
     4801 gccaggcgtc gagcgcctgc ttggtgagct cgtaggcgac cgtctcctcg gggacatgcg
     4861 cgcgagacgt gcggggcccc gggtcgaaca cgctcaactc gccgaccatg tccccgacgc
     4921 cgaggagcga caacaggttc tcgcgtccgt cgcgcgcggt gcgcgcgagc ttgaccttgc
     4981 ccttgacgat gacgtagagg gcgtcgccgg gggcgccctc gaagaacatc accgcgccgc
     5041 gggagaactc gcgccgcgtc atgaccgaga tcagcgaacg agccgtctcc gggtccatgg
     5101 cgctcagcaa cagggaagaa cgcaaagcgt tctcttcctg cgcccggtcc tgtgccacgt
     5161 ccgcctccga cctaccgtgc cgcgcccatt gcgtcgatca tgtccgccgt tacaccctca
     5221 aggttggcag aaagtcggcg ttcgtacgag tccaatcgcc cgtcgatctc ttccaggcgt
     5281 tgaaccaagt ctgccgtggc gctctctgcg ccgggccaaa tgacctcggc gagttcgctc
     5341 gacgtcggcg ccccagaggc gagggcctcc cacgcggctt ccacgttcgc gtccgggtcg
     5401 gtcgcgccag ccatctgggc aatcgacaac tcgcgacgcg acgcgatgag ccgacgccac
     5461 cagcggaccc tggcgagttc gcgcctcagt tcgcgccgtt gctgccgaag atcggcaacc
     5521 gtctgcgcga ccgcatgcgc catgcccgct ccctccccac cgcgatccac gggaccgcgc
     5581 tcctaggact tatcggcccg agcgagccaa ggcttgaggg tcacctaggc tggccgcatg
     5641 gcctcgcgcc ccccgcttac ggtcgccatt gccggcgacg aacctcccga cgcggcactg
     5701 gtgcgacgcg ctcggcgcgt caatcggatc ctcgcgggcg agtacccgga cgctcggtgc
     5761 gaactggact accagaaccc cttcgaactc atcgtggcca ccgtcctttc cgcgcagtgc
     5821 acggatgtcc aggtcaacct cgtcacgccc gcgctcttcg ccgcgttccc cgacgccgag
     5881 gcgctcgccg ccgccccgcg cgaacggctc gaggccctca tccgctcgac gggcttcttc
     5941 cacgccaagg cccagtccct gctcgaactc agccgcgcgc tggtggagcg cttcggggcg
     6001 gaagtcccgc cccacctcga cgacctcgtc accctgaggg gcgtcggccg aaagac
//
LOCUS       DASVKP010000089         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1018618, whole genome shotgun sequence.
ACCESSION   DASVKP010000089 DASVKP010000000
VERSION     DASVKP010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018618"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VF362_01965"
     CDS             <1..607
                     /locus_tag="VF362_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEX7589724.1"
                     /translation="GLDWVARLLRARGTVLPLAAEPLEISASVDVGGETVTVRGQVAV
                     ATAPGRISRLWLRPEAPHVPPETLAAIRSADLVALGPGSWYSSVLPHFLVAQVANELV
                     VAGPRCVLSLNIAHLDGETEGTDRVDDVRALQTVAPDFVPAVVIVDEGHADDPGLAAL
                     IEDWGSILIVERLLQEGTLDRHDADRFAGAYGRAAAILGRG"
     gene            640..1620
                     /gene="whiA"
                     /locus_tag="VF362_01970"
     CDS             640..1620
                     /gene="whiA"
                     /locus_tag="VF362_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEX7589725.1"
                     /translation="MALTAQVKEELARVVVEKPSCRKAEIATMLRFAGGLHIVSGRIV
                     IEAELDTSAGARRLRQFLSEVYGHSSEIIVVSGGALKKGNRYVVRVVKDGESLARQTG
                     LLDHRGRPVRGLPPQVVGGSIEDTISAWRGAFLAHGSLTEPGRSSALEVTSPGPEAAL
                     ALVGAARRLGISAKSREVRGVDRVVIRDGDAIAAMLTRLGAHDSVLEWEEKRTRREVR
                     GTANRLANFDDANLRRSARAAVAAGARVARAFEILGDAVPGHLREAGELRLQHKDASL
                     EELGKMAEPALTKDAVAGRIRRLLATADKHAEELGIPDTEASLSPEMLDL"
     gene            1778..>2359
                     /locus_tag="VF362_01975"
     CDS             1778..>2359
                     /locus_tag="VF362_01975"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX7589726.1"
                     /translation="MTVKVGINGFGRIGRNFFRAVLEQGADIEIVGVNDLTDNKTLAQ
                     LLKFDSVLGRLPQDVTYTDTTITVGGKTFAASAEKDPANLDWGKLGADVVIESTGFFV
                     DAEKAKAHIAAGAKKVIISAPGKNEDATFVMGVNHTDYDPAKHNIISNASCTTNCLAP
                     MAKALNDSVGIEKGLMTTIHAYTADQNLMDGPHR"
BASE COUNT          406 a          795 c          807 g          351 t
ORIGIN      
        1 ggggctcgat tgggtcgccc ggctccttcg cgctcgcgga acggtgcttc ccctcgcggc
       61 ggagcctttg gagatcagcg cgtcggtcga cgtcggcggc gagaccgtca ccgttcgcgg
      121 ccaggtcgcc gtcgcaacgg cgccgggacg catctccagg ctgtggctcc ggccagaggc
      181 gccgcacgtc cccccagaga cccttgccgc gatccgcagc gccgatctgg tggccctcgg
      241 cccaggaagc tggtactcgt ccgtcctgcc ccatttcctg gtcgcacagg tcgcgaacga
      301 actcgtcgtc gccgggcccc gatgcgtcct ctccctgaac atcgctcacc tcgacgggga
      361 gacggagggg accgacaggg tcgacgacgt cagggcgctc cagacggtgg cgcccgactt
      421 cgtgcccgcg gtagtcatcg tcgacgaggg tcatgccgac gatcccgggc tcgcagcctt
      481 gatcgaggac tggggatcga ttctcatcgt cgagcgcctg ctccaagagg ggacacttga
      541 tcgccacgac gctgatcgat tcgccggcgc gtatgggagg gccgcggcga tcctcggacg
      601 gggatgaggc ccggccgcca aaggtggcag gatacgttca tggcattgac ggcgcaggtg
      661 aaggaggaac tcgcccgcgt cgtcgtggag aagccatcgt gccgcaaggc ggagatcgcg
      721 accatgctgc gcttcgctgg cggactccac atcgtttccg gccgcatcgt gatcgaggcg
      781 gaactcgaca cgtcggccgg tgcccgccgc ctccgccagt ttctcagcga ggtctatggc
      841 cactcctccg agattatcgt ggtctccggc ggagccctca agaagggcaa tcgctacgtc
      901 gtcagggtcg tcaaggacgg cgagtcgctc gcgaggcaaa ccggccttct tgaccatagg
      961 gggcgtcccg tgcggggcct gcctccccag gtcgtgggcg gttccatcga ggacacgatc
     1021 tcggcgtggc ggggggcctt cctcgcgcac ggttcgctga ccgagccggg gcgctcatcg
     1081 gcgctcgagg tgacctcacc cgggccggaa gcagcgctcg cgctcgtggg ggccgcgcgg
     1141 cgcctgggaa tttccgcgaa gtcgcgggag gtccgcggcg tcgaccgcgt cgtgattagg
     1201 gatggcgacg cgatcgcggc catgctcacg cgcctcggcg cgcacgactc cgtcctcgag
     1261 tgggaagaga agcggacgcg gcgcgaggtc aggggcacgg cgaaccggct tgccaacttc
     1321 gacgacgcca acttgcggcg gagcgcacgg gccgcggtgg cggccggggc tcgcgtcgcg
     1381 agggcgttcg agatcctggg cgacgcggtg cccggtcact tgcgagaagc gggggagttg
     1441 cgtcttcagc acaaggacgc gtccctcgag gagctgggga agatggcgga gccggccctg
     1501 acgaaggacg cggtggcggg gcggatccgg cgcctcctcg cgaccgcaga caagcacgcg
     1561 gaggaacttg ggattcccga cacggaggcg tccctcagcc cggagatgct cgacctctag
     1621 ggctccccgg ggaccttcgt caccttcccg tcaaggccaa catagtaggc tgaacacagt
     1681 ttcccgccca gtgcggggac gcgcacgtcg ccgtgcgaca cccttagcgg aatcccacgt
     1741 cggtgcgtgc cgacgcgagc caaggagaga gagaaacatg accgtcaagg tcggcatcaa
     1801 cggcttcggc cggattggac gcaacttctt ccgggccgtg ctcgagcagg gcgcggacat
     1861 cgagatcgtg ggggtgaatg acctcaccga caacaagacg ctcgcacaac tgctcaagtt
     1921 cgactccgtc ctcgggcgcc tgccgcagga cgttacctac accgacacca ccatcaccgt
     1981 gggcggaaag accttcgcgg cctcggccga gaaggacccc gccaacctgg actggggcaa
     2041 gctcggcgcg gacgtcgtca tcgagtccac cggcttcttc gtcgacgcag agaaggccaa
     2101 ggcgcacatc gcggccggag ccaagaaggt catcatctcc gctcccggca agaacgagga
     2161 cgcgacgttc gtgatgggcg tgaaccacac ggactacgat cccgccaagc acaacatcat
     2221 ctcgaacgcg tcgtgcacca cgaactgcct cgcccccatg gcgaaggcgc tcaacgactc
     2281 ggtgggcatc gaaaagggtc tcatgaccac gatccacgcc tacaccgcag accagaacct
     2341 catggacggc ccgcaccgc
//
LOCUS       DASVKP010000090         3211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_609246, whole genome shotgun sequence.
ACCESSION   DASVKP010000090 DASVKP010000000
VERSION     DASVKP010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609246"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1104
                     /locus_tag="VF362_01980"
     CDS             157..1104
                     /locus_tag="VF362_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX7589727.1"
                     /translation="MAWTTANIPSLTGRVALVTGANSGLGLETARALAGAGAHVILAA
                     RTAEKARAAEPNIRASFPEASLEILAMDLASQASIKAAASAVAKKHPTLDILVNNAGV
                     MALPEGRTEDGYETQLGVDHLGHWTLTALLLPSLLKADAARVVTVTSMARHQGKALDE
                     KDPYHEGAYDPWRAYGDAKLANYHFAIGLQREFERHGARASSLTAHPGLSRTNLYDVT
                     DREGGAGSSGTLLHKVTERRGMTSARGALPQLRAATDPRARGGQLYAPRFVATGAAVR
                     RPILRRLGLGAAIEALWAVSERETGVALPFEQGTGSAAR"
     gene            1121..2767
                     /locus_tag="VF362_01985"
     CDS             1121..2767
                     /locus_tag="VF362_01985"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX7589728.1"
                     /translation="MPSLRLALAQVNPCVGDLAGNARIVLEHCRAAHGAGADVVAFPE
                     MVITGYPIEDLALRKSFQRAAERIVREIAASLAADGMGAMTVILGTLGVAGDGRPANQ
                     AVVIERGEVVARYTKHHLPNYGVFDERRIFAPGDETCVVEARGRRLGIVICEDIWQDG
                     GPVSTMVAADVQALVVINGSPFEEGKGHVRHELAARRAREVRAPVGYVNLWGGQDDLV
                     FDGQSFVVGADGALLASAPAFADALLLWDVPGDGEAPSPSAIFPPLASDAEVYAACVT
                     GLRDYVRKNGMSKVVLGLSGGIDSALAATIAADALGGENVVGVSLPSRYSSDHSKDDA
                     AVLAATLGAEYRVIAIEPFVSGFEATLGLQGVAAENLQARVRAIILMGISNSEGPLLL
                     ATGNKSELATGYTTIYGDAAGGFAPLKDLDKSRVWDLARWRNADAAARGKTPPIPVNS
                     IEKPPSAELRPNQVDQDSLPPYDLLDEVLDAYVEHAEGRAELIARGFDEAVVDRVLSL
                     TDRAEWKRRQYPPGPKVTALAFGRDRRLPITSRWREKVEP"
     gene            2764..>3211
                     /locus_tag="VF362_01990"
     CDS             2764..>3211
                     /locus_tag="VF362_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX7589729.1"
                     /translation="MSVTPERKKIRIHHFAQMKAAGEKITMLTAYDFTTAKIFDAAGV
                     DVLLVGDSLGDNVLGHGNTSAVTLDEMIPHVRAVTRGTERAFVVADLPFGSYEISPEQ
                     AVASSIRLVKEGGAHAVKFEGGRRVAAQAKAVVDAGIPFVGHLGFTP"
BASE COUNT          492 a         1185 c         1092 g          442 t
ORIGIN      
        1 ctcgacactc cggagcacgt actcctgctg cttgtccatg gctctatcct gccccacgga
       61 tgttacggat ggatgacacg gcgcgggccg cttccccttg gccccaaggc gacaaaccca
      121 ccacactggg gggaggcgtc aacacgagga gcaaccatgg cctggaccac cgccaacatc
      181 ccgagcctca ccggtcgcgt agcgctcgtc accggggcga acagcgggct cggactcgag
      241 accgcgcgcg ctctcgcggg ggccggggcg cacgtcatcc tggccgcgcg gaccgccgaa
      301 aaggcccgcg ccgccgagcc aaacatccgt gcgtccttcc ccgaagcctc cctcgagatc
      361 ctcgcgatgg accttgcttc gcaggcctcg atcaaggccg ccgcgagcgc cgtcgcgaag
      421 aagcacccca cgctcgacat cctcgtgaac aacgcgggcg tgatggcgct cccggagggc
      481 cggaccgagg acggatacga gacccaactc ggcgtcgacc acctgggcca ctggaccctc
      541 acggcgctcc tcctgccctc cctcctcaag gccgacgccg cgcgcgtcgt caccgtcacg
      601 tccatggcgc gccaccaggg caaggcgctc gatgagaagg acccgtacca cgagggcgcc
      661 tacgacccgt ggcgcgcgta cggcgacgcc aagctcgcca actaccactt cgcgatcggg
      721 cttcagcgcg agttcgagcg ccacggcgcc cgggcctcga gcctcaccgc tcacccaggc
      781 ttgtcacgca cgaacctcta tgacgtcacc gaccgggagg gcggggcggg ctcgagcggc
      841 accttgttgc acaaagtcac cgagcgtcgg gggatgacgt cggcgcgagg cgccctgcct
      901 cagctccggg cggcgaccga cccccgggcg aggggcggac agttgtacgc gccgcgcttc
      961 gtcgcgacgg gggcggcggt tcggcgaccg atccttcggc gccttggcct gggagcggcg
     1021 atcgaggccc tgtgggccgt ctccgagagg gaaaccggcg tcgcgctccc gttcgagcag
     1081 ggcacggggt ccgctgcgcg ctaacgcgta ggctcatccc atgccttcgc ttcgcctcgc
     1141 cctggcccag gtcaacccgt gcgtcgggga cctcgcgggc aacgcccgca tcgtcctgga
     1201 acactgccgc gcggcccacg gcgccggggc ggacgtcgtc gcgtttcccg agatggtcat
     1261 cacgggctac ccgatcgagg acctcgccct caggaaatcc ttccagcggg cagccgagcg
     1321 catcgtcagg gagatcgccg cgagcctcgc ggccgacggc atgggcgcga tgaccgtgat
     1381 cctcgggacg ctcggcgtcg cgggggacgg gcgcccggcg aaccaggccg tcgtcatcga
     1441 gcgcggcgag gtcgtcgccc ggtacaccaa gcaccacctg ccgaactacg gggtcttcga
     1501 cgagcggcgc atcttcgcgc cgggcgacga gacgtgcgtc gtggaggccc gtggccgacg
     1561 cctcggaatc gtcatctgcg aggacatctg gcaggacggc ggtccggtat ccacgatggt
     1621 cgccgccgac gtgcaggctc tcgtcgtcat caacggctcg cccttcgagg agggcaaggg
     1681 ccacgtccgg cacgagctcg cggcgcgccg cgcgcgcgag gtccgggcgc cggtcggcta
     1741 tgtgaacctg tggggcggcc aggacgacct cgtcttcgac ggccagtcct tcgtcgtggg
     1801 cgcggacggc gcgctcctcg cctccgcccc cgccttcgcg gacgccctgc tcctgtggga
     1861 cgtgcccggg gacggggagg cgccctcacc gtcggcgatc ttccccccgc tggcgagcga
     1921 cgccgaggtg tacgccgcct gcgtcacggg ccttcgcgac tacgtgcgca agaacggaat
     1981 gagcaaggtc gtgctggggc tctcgggagg catcgactcc gcgctcgcgg cgacgatcgc
     2041 ggcggacgcc ctgggtgggg agaacgtcgt cggcgtctcg ctgccgtccc ggtactcctc
     2101 ggaccactcc aaggacgacg ccgcggtgct cgccgcgacc ctcggcgccg agtaccgggt
     2161 catcgcgatc gagccgttcg tctccgggtt cgaggcgacg ctgggactgc agggggtcgc
     2221 ggcggagaac ctccaggccc gcgtccgggc catcatcctc atgggcatct ccaactccga
     2281 ggggccgctg ctgctcgcca cgggaaacaa gagcgagctc gccaccggat acaccacgat
     2341 ctacggagac gcggcgggcg gcttcgcgcc gctcaaggac ctcgacaagt cgcgcgtgtg
     2401 ggacctcgcc cgatggcgca acgccgacgc ggccgcgcgc ggcaagacgc cgcccatccc
     2461 cgtcaactcc atcgagaagc ccccgagcgc cgaactgcgt ccgaaccagg ttgaccagga
     2521 ctcgctgccc ccgtacgacc tcctcgacga agtcctcgac gcctacgtcg aacacgccga
     2581 gggccgcgcg gaactcatcg ctcgcgggtt cgacgaggcc gtcgtcgacc gcgtcctgtc
     2641 gctgaccgac cgcgccgagt ggaagcgccg gcagtatcct ccgggcccca aggtgacggc
     2701 gctggcgttc gggcgcgacc ggcgtcttcc catcaccagc aggtggcgtg agaaggtgga
     2761 gccatgagcg tcaccccgga gcgcaagaag attcgcatcc accacttcgc ccagatgaag
     2821 gcggcgggcg agaagatcac catgctcacc gcgtacgact tcacgaccgc gaagatcttc
     2881 gacgccgcgg gcgtggacgt gctactcgtc ggcgactcgc tcggcgacaa cgtccttggc
     2941 cacggcaaca cgtcggccgt cacgctagac gagatgatcc cccacgtccg cgcggttacc
     3001 cgcgggaccg agcgcgcctt cgtcgtcgcc gacctgccgt tcgggagcta cgagatcagc
     3061 cccgagcagg ccgtcgcgtc ctccatccgg ctcgtcaagg agggtggcgc ccacgcggtg
     3121 aagttcgagg gtgggcgccg ggttgccgcc caggcgaagg cggtcgtcga cgcggggatt
     3181 cccttcgtgg gccacctcgg cttcaccccg c
//
LOCUS       DASVKP010000091         1976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_113275, whole genome shotgun sequence.
ACCESSION   DASVKP010000091 DASVKP010000000
VERSION     DASVKP010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113275"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="VF362_01995"
     CDS             <1..1183
                     /locus_tag="VF362_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589730.1"
                     /translation="LSARVSVAWTSRVLQSRRAREAALAAILLGALVATPVAYAVVSE
                     GLDTVLSIDVPALLEALRWTPVGLPVAAVSAAATGAWWSVAWRLGASLAWVLLLHGVW
                     RANVAYTLVHPTYRGGGGRARDDAVLAAAKRSEASRLPRPVRAQAAVASRAASAQRAV
                     ASRALRYWFTDPRYLSGLVSVMVFPALFFTLVYPIFGSPIAVVMSIPVLVAGTIGWAR
                     HNDVAFDSTALWLDVVSGRLGREVMRGRVAATLTWAVPVVTVAGLVALGLAGRWDLAP
                     AEFGAIAGVLGTSLGVSAISAVALPYRAPAPGENPFSAEVGSVGAGFLAQVVSSISAW
                     IVAVPVTLPLVAAIRWDARIGWLGLVLGLATGVGVLVVSTRWAGRLYDRKSGRLVTAV
                     A"
     gene            1229..1510
                     /locus_tag="VF362_02000"
     CDS             1229..1510
                     /locus_tag="VF362_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HEX7589731.1"
                     /translation="MTEQETRPSEQTRASTTTIARPDTQVAEPGDSERFSHYVRKEKI
                     VEAAVAGTPVIALCGKVWVPNRDPQRFPVCPACQDILDKAFPPDDAPGE"
     gene            1564..1812
                     /gene="clpS"
                     /locus_tag="VF362_02005"
     CDS             1564..1812
                     /gene="clpS"
                     /locus_tag="VF362_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX7589732.1"
                     /translation="MGRLDDAWVTIVWNDPVNLMSYVTHVFQSYFGWTRVKAERKMRE
                     VHEKGRSVVSTGGREQMEVDVQAMHGYGLWATMQRSGE"
BASE COUNT          239 a          661 c          743 g          333 t
ORIGIN      
        1 cctttcggcg cgagtatcgg tggcatggac gtcgagggtg cttcagtccc ggcgggcgcg
       61 ggaggcggcc cttgcggcga ttctccttgg cgctctcgtg gcgacgcccg tcgcgtacgc
      121 cgtcgtctcc gagggtctcg ataccgtcct gagtatcgac gtccccgccc tcctcgaggc
      181 gctgcggtgg acccccgtcg ggctacccgt ggcggcggtg agcgcggcgg cgacgggcgc
      241 gtggtggtcg gtggcgtgga ggctcggggc ctccctcgca tgggtcctgc tccttcacgg
      301 ggtgtggcga gccaacgtgg cgtatacgct cgtccatccg acctaccgcg ggggcggggg
      361 gcgcgcgcgc gacgacgccg tcctcgcggc ggccaagcgt tccgaggcta gtcgccttcc
      421 gcgaccggtg cgggctcagg ccgccgtcgc gtcgcgggcg gctagcgccc agcgcgccgt
      481 cgcgtcgcgg gcgcttcggt actggttcac ggatccccgc tacctcagcg ggctcgtctc
      541 ggtgatggtc ttcccggcgc tgttcttcac gctcgtctat ccgatcttcg gctctcccat
      601 cgcggtcgtc atgtccatac cggtgctggt cgcgggaacg atcgggtggg ctcgccacaa
      661 cgacgtcgcg ttcgattcga cggccctgtg gctcgacgtc gtctccggca ggctcggacg
      721 ggaggtcatg cgcgggcgtg tcgctgcgac gctgacgtgg gcggttcccg tcgtgaccgt
      781 ggccggcctt gtggcgcttg gcctcgcggg aaggtgggac ctcgcgcctg cggaattcgg
      841 ggcgatcgcc ggagtcctcg gcaccagcct cggcgtatcg gcgatcagcg cggttgccct
      901 cccgtatcgg gcccccgcgc ccggggagaa cccgttctcg gccgaggtgg gatcggtggg
      961 ggcgggcttc ctcgcccagg tggtctcgtc catcagtgcg tggatcgtgg cggttcctgt
     1021 gacgcttccc ctggttgccg cgatccgctg ggacgcccgc atcggctggc tcggcctcgt
     1081 gcttgggctc gcgaccgggg tcggggtgct ggtggtgtcg acccgatggg ccgggcgact
     1141 gtacgaccgc aagagcgggc gcctcgtgac cgcggtggcc taggaccagc cgcccggggg
     1201 gcgagtggcc ccggcgtagc atgggcccat gaccgagcag gagactcgcc ctagcgagca
     1261 gacgcgcgcc tcgaccacca ccatcgcccg tccggacacc caggtcgcgg agccggggga
     1321 ttccgagcgt ttctcgcact acgtccgcaa ggagaagatc gtcgaggcgg ccgtcgcggg
     1381 cacgcccgtc atcgcgctgt gcggcaaggt ctgggtcccg aaccgcgatc cgcagcgatt
     1441 ccccgtctgc cccgcgtgcc aggacatact cgacaaggcg ttcccgcccg acgacgcgcc
     1501 cggcgagtag ggcggggggt gggcgtgagc gcagacccgg caacgggtag gtccacgagt
     1561 gtcttgggtc gcctcgacga cgcgtgggtc acgatcgtct ggaacgatcc ggtcaacctc
     1621 atgagctacg tgacgcacgt gttccagtcc tactttggct ggacgcgcgt caaggccgag
     1681 cggaagatgc gcgaggtcca cgagaagggc cggtcggtcg tttcgaccgg tggacgcgaa
     1741 cagatggagg tggacgtgca ggcgatgcac ggctacggcc tgtgggcgac gatgcaacgg
     1801 agcggcgagt gaaggcgttc agggcgcgcc ggggcgtcgc ggtcgccgaa ctcgatcacg
     1861 acgagcgcgc cgtcgtggcg cggatcgttg ccgacgtcgg gcttctcctc ggcggcgagg
     1921 tcttcggcat ggagagcacc ccctggacgg ccgagactcc ccgggatctc ctcgaa
//
LOCUS       DASVKP010000092         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1186272, whole genome shotgun sequence.
ACCESSION   DASVKP010000092 DASVKP010000000
VERSION     DASVKP010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186272"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VF362_02010"
     CDS             <1..139
                     /locus_tag="VF362_02010"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HEX7589733.1"
                     /translation="IIATQVDRAEDAGDGTFYYAQDYHQAYLHKNPAGYCNHGFCQVA
                     Y"
     gene            complement(136..1107)
                     /locus_tag="VF362_02015"
     CDS             complement(136..1107)
                     /locus_tag="VF362_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEX7589734.1"
                     /translation="MAHYDLPLDELREYRPVIEEPADLDAFWADTVAEARAFGGDPIL
                     AKVDPPMPNVEVWDVTFPGFGGAPIKAWYSRPAGDRSDLPVIVQYQGYNGGRGLAWEH
                     TFWPSAGYAHLMMDSRGQGSGWGAGGETPDPGGTGAAGAGYMTRGILDPADYYYRRLF
                     TDAVRAIDAVRKLPGIDQGRIFAAGISQGGGVAIAVAALVPGLAAALVDVPFLCHYRR
                     ALAVIDTDPYAEITRYLSVHRDHTERAFETLSYFDGATLGKRATAPGLFSVALMDDTC
                     PPSTVFAAFNAWGGHPKEILVYPYNRHEGGGAYQIKHQWQYVRRMTA"
     gene            complement(1175..1248)
                     /locus_tag="VF362_02020"
     tRNA            complement(1175..1248)
                     /locus_tag="VF362_02020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1212..1214),aa:Leu,seq:taa)
     gene            complement(1278..2228)
                     /locus_tag="VF362_02025"
     CDS             complement(1278..2228)
                     /locus_tag="VF362_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX7589735.1"
                     /translation="MRVAAIDCGTNSLRLLVADVDPATGTLRDVTREMVVVRLGEGVD
                     RTGLFAPAALERTFAAVDAYAETCRALGAEAIRFVATSASRDAANSDLLVAGVERRLG
                     VVPEVISGEEEARLSFAGATRALAPGLEAPFLVVDLGGGSTELVLGGREPEYAHSMNV
                     GCIRLAERHLSADPARASEIELAVRDIREALDAAARDVPLARAGTLVGVSGTVTTITA
                     RVLGLDAYRPERIDGSILRIDDVVAAADDLLGMTRGARAALPFMHPGRVDSIGAGALV
                     WREVVLRVRREVEDAGGSLETVATSEHDILDGIALALVPA"
     gene            complement(2259..>2731)
                     /locus_tag="VF362_02030"
     CDS             complement(2259..>2731)
                     /locus_tag="VF362_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEX7589736.1"
                     /translation="REQLGREPRGVVAIPARCVCGNPIVVRTLPRLDDGTPFPTSYYL
                     SHPGAIAALSTLEAEGAMRAMNERLDEDSQLGFRYLAAHESYLADRAELGEVPEIDDI
                     SAGGMPTRVKCLHALAAHALAKGPGVNPFGDEALEAIRDTWRRDRCTCATPRSY"
BASE COUNT          418 a          978 c          953 g          382 t
ORIGIN      
        1 gatcatcgcc acccaggtgg accgggcgga ggatgccggc gacgggacgt tctactacgc
       61 ccaggactac caccaggcgt acctccacaa gaaccctgcg ggctactgca accacgggtt
      121 ctgtcaggtc gcctactagg cggtcatccg gcgaacgtac tgccactggt gcttgatctg
      181 gtatgcgccc ccgccctcat gccggttgta cggatagacc agaatctcct tggggtgccc
      241 accccacgcg ttgaaggcag cgaacaccgt ggatggcggg cacgtgtcgt ccatcagcgc
      301 cacggaaaac aggccggggg ccgtggcccg cttgcccagg gtggcgccgt cgaagtacga
      361 aagggtctcg aaggcgcgct ccgtgtggtc gcggtggacc gagaggtagc gcgtgatctc
      421 ggcgtaggga tcggtatcga tgacggcgag cgcccgccgg tagtggcaga ggaatggcac
      481 gtcgacgagg gccgccgcga ggccgggaac gagggctgcc accgcgatcg cgacgccccc
      541 gccttgggag atccccgcgg cgaagatgcg gccctggtca atccccggca acttccgcac
      601 ggcgtcgatc gcccgcacgg cgtccgtgaa gagccgccgg tagtagtagt cggccgggtc
      661 gaggatgccg cgcgtcatgt acccggcgcc cgccgctccc gtaccgccgg gatccggcgt
      721 ctcgcccccc gcgccccagc cggagccctg cccgcgcgag tccatcatca ggtgggcgta
      781 gcccgcagag ggccagaagg tgtgctccca ggcgagcccg cggccgccgt tgtagccctg
      841 gtattgaacg atgacgggaa ggtcgctccg gtcgcccgcg gggcgcgagt accacgcctt
      901 gatcggggcg cccccgaagc cggggaacgt cacgtcccag acctcgacgt tcggcatcgg
      961 cgggtccacc ttcgcgagaa tcggatcgcc gccgaaggct cgggcctccg cgacggtgtc
     1021 cgcccagaac gcgtccagat ccgcgggttc ctcgatgacg ggccggtact cgcgaagctc
     1081 gtccagcggg aggtcgtaat gcgccacgtc cgtctcctcg cctcgccggg accccgctcc
     1141 atcggatcgt cgtcatgcta caacgcgacg gatgtgcccc cggcagggct cgaacctgcg
     1201 cttggccgat tttaagtcga ctgcctctac cagctgggct acgggggccc gagtcgattg
     1261 tcccaggccg agcccactta cgcgggcacc agcgcgagcg cgatcccgtc gagtatgtcg
     1321 tgctcgctcg tcgcgacggt ctcgagcgag ccgcccgcgt cctcgacctc tcgcctgacc
     1381 cgcagcacaa cctcgcgcca cacgagcgcc cccgccccga tcgagtcgac gcggccggga
     1441 tgcatgaacg gaagggcggc cctggcgccc ctggtcatgc caagcagatc gtccgccgcc
     1501 gcgacgacgt catcgatccg caggatcgag ccgtcgatgc gctccggccg gtacgcgtcg
     1561 agccccagga cgcgcgcggt gatggtcgtg acggttccgg agacgccgac gagggtgccc
     1621 gcgcgcgcga gcggcacatc cctcgccgcc gcgtcgagcg cttctcggat gtcgcgaacg
     1681 gccagttcga tctcactcgc gcgtgccgga tccgcggaga ggtggcgctc cgcgagccgt
     1741 atgcatccca cgttcatcga gtgggcgtac tcgggctcgc ggccgccgag gacgagttcg
     1801 gtcgagccgc ccccaaggtc caccacaagg aagggcgcct ccaggcccgg cgcgagcgcg
     1861 cgggtcgcac ccgcgaagga caggcgtgcc tcctcctccc ccgagatgac ctcgggcacg
     1921 acgccgaggc gccgctcgac ccccgcgacg aggagatcgc tgttggccgc atcccgcgac
     1981 gccgaggtcg cgacgaatcg gatggcctcc gccccgagcg cccggcacgt ctcggcgtaa
     2041 gcgtccaccg cggcgaaggt ccgctccagc gccgcgggtg cgaagaggcc cgttcgatcg
     2101 acgccctcgc cgaggcgcac cacgaccatc tcgcgggtga cgtcgcgaag ggtccccgtg
     2161 gcagggtcga cgtcggcgac aagcaggcgc agggagttgg tcccgcaatc gatggccgcg
     2221 actcgcatgg cgacagcgta acgggggcgg cggacccgtc agtacgagcg aggcgtcgcg
     2281 caggtacacc ggtcgcggcg ccacgtgtcg cggatcgcct cgagcgcctc gtcgccgaag
     2341 ggattgactc cgggaccctt ggccagcgcg tgcgccgcga gcgcgtggag gcacttcacc
     2401 cgcgtgggca tgccgcccgc gctgatgtcg tcgatctcgg gaacctcacc caactccgcc
     2461 cggtcggcga ggtaggactc gtgcgcggcg aggtatcgga atcccaactg cgagtcctcg
     2521 tcgagccgct cgttcatcgc ccgcatcgct ccctcggcct cgagggtgga cagggccgcg
     2581 atggcacccg gatggctgag gtagtaactc gtggggaacg gcgtgccgtc gtcgaggcgc
     2641 ggaagggtgc ggacgacgat cgggttgccg cagacgcacc tcgcggggat cgcgacgacg
     2701 ccccggggct ccctgcccaa ctgctcgcgc a
//
LOCUS       DASVKP010000093         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1594487, whole genome shotgun sequence.
ACCESSION   DASVKP010000093 DASVKP010000000
VERSION     DASVKP010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594487"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VF362_02035"
     CDS             <1..185
                     /locus_tag="VF362_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589737.1"
                     /translation="EKVTSLEATFDTVNPADLTRRIVAIQTRLITLAAAKTAAQTLTV
                     SRAKPDEAREPLSRAS"
     gene            complement(326..1591)
                     /locus_tag="VF362_02040"
     CDS             complement(326..1591)
                     /locus_tag="VF362_02040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7589738.1"
                     /translation="MPDANPGRAHRRRAVPDFPPLGTAPPLATPTGRAPNVSLARANA
                     VLVLLAIAAFAFVTSEVLPFGLITLISTDLHRSNSEVGLLVTAYAAVVVATSIPLAHL
                     TRSIPRRQLLSATLLVYVAGMLLASLGGGFASLLAGRMLTAFAQAQFWAVVTATAAGL
                     FPAHVRGKIVSRLLLGPAVAGLLGLPIATWLGQHFGWRAPFYVLACMGVVLAILIALL
                     VPRYKPADGAAARGVTPSVRRFVFILAITVTAILGTNTIGPYVTPYLRDVVGFSSSSF
                     PVLFTLSGIAGLVAMIVVGRYLDRLPMGMIAIGLGMLLIGWGGVAVAGTSKPAAVILY
                     CLAVAGVSFIVGGLANRALQTSPGSTDIGIATQGSFYNTGTALGSLVGSGVLAAHDPQ
                     SLPVLAAGFLSLALVLVVLERRFGGPRTR"
     gene            complement(1717..>2157)
                     /locus_tag="VF362_02045"
     CDS             complement(1717..>2157)
                     /locus_tag="VF362_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7589739.1"
                     /translation="PTPTTTTPPPPVPTPTTTTPPPAAPTAAVTYTVVAGDTLYKIAT
                     KFHTTVSAIASANGITNVNLIRVGQKLKIPSSGTTATTPTTTTPPPAAPVAATTVTYT
                     VVAGDTLGKIAAKYHTTVMAIAVANKIKNVNLIVVGQKLTITTG"
BASE COUNT          365 a          671 c          835 g          286 t
ORIGIN      
        1 ccgagaaggt aacctcactg gaagccacgt tcgataccgt caacccagcc gacctgaccc
       61 gcaggatcgt cgccatccaa acccgcctca tcaccctcgc cgcagcaaaa accgccgccc
      121 aaaccctcac tgtttcgcga gcaaaaccag atgaggcacg cgagcccctt tcgcgagcat
      181 cttgacatga ggcactacgc ggtacggccc ggatgggccg taccgttcgc atgtgtgggc
      241 tagatcgggg cgaggatgtg ggctccttcg tcaggtaggc gcccggcggg aagccgggcg
      301 ccgggcccct cacccagtag tgcccctaac gcgtccgggg gcccccgaag cgtcgttcga
      361 gcaccacgag aacgagcgcg agcgagagga agcccgcggc gaggacgggg agggactgcg
      421 ggtcgtgggc ggccagcacg ccggaaccaa cgagcgaacc cagcgccgtc ccggtgttgt
      481 agaacgaacc ctgggtggcg atcccgatgt cggtggagcc gggggaggtc tggagcgccc
      541 ggttcgcgag gccccccacg atgaagctca cgcccgcgac cgcgaggcag tagaggatga
      601 cggcggcggg cttcgacgtc cccgcgaccg caacgccccc ccagccgatg aggagcatcc
      661 cgagcccgat cgcgatcatg cccatcggaa ggcgatcgag gtaccgaccg acgacgatca
      721 tcgcgacgag ccccgcgatc cccgacaggg tgaagagcac ggggaacgac gatgaggaga
      781 agccgacgac gtcgcgcaga tagggcgtga cgtagggtcc gatcgtgttg gtcccgagga
      841 tggccgtcac ggtgatggcg aggatgaaga cgaagcgtcg cacgctcggg gtgacccccc
      901 gggctgcggc gccgtcggcg ggcttgtacc gcggaacgag gagcgcgatc aggatcgcga
      961 gaaccacgcc catgcacgcg aggacgtaga agggcgcccg ccagccgaag tgctggccga
     1021 gccaggtcgc aataggcagg ccgagcaggc ccgcgaccgc ggggccgagg aggagccgcg
     1081 agacgatctt gccgcgcacg tgcgcgggga agaggccagc ggcggtcgcg gtcacgaccg
     1141 cccagaactg cgcctgggcg aaggccgtga gcatccggcc cgcgaggagg ctggcgaagc
     1201 ccccgccgag gctcgcgagc agcattccgg cgacgtagac gagcagcgtt gcggagagca
     1261 gttgccggcg cgggatgctt cgcgtgaggt gcgcgagggg aatggaggtg gcgacgacga
     1321 cggcggcgta cgccgtcacg aggaggccga cctcggagtt actgcggtga aggtccgtgg
     1381 agatgagcgt gatgagtccg aacgggagga cctcgctcgt gacgaaggcg aacgccgcga
     1441 tggcgaggag caccagcacc gcgttggcgc gggcgagcga gacgttcggg gcgcggccgg
     1501 tgggcgtcgc gagcgggggt gcggttccga gcggcgggaa gtccgggacc gcgcgccgcc
     1561 ggtgggcgcg gccggggttc gcgtcaggca tgggtgcggg actcctggat ggggatggcc
     1621 caagcgtctc acgccgcgca atgcgggcgc gacacgaagc gggccgcgcg agcggcggag
     1681 gcgcgcaggg gggaggggtg cgcccccccg ggactcctag cccgtcgtga tggtcagttt
     1741 ctggccgaca acgatcaggt tgacgttttt gatcttgttc gcgaccgcga tcgccatgac
     1801 cgtggtgtgg tacttcgccg cgatcttgcc tagcgtgtca cccgccacga cggtgtaggt
     1861 gaccgtcgtg gccgcgacgg gtgccgccgg gggcggcgtg gtggtggtcg gggtggtcgc
     1921 cgtggttccg gaactcggga tcttcaattt ctgcccgacc cggatcaggt tgacgttggt
     1981 gatgccgttc gccgacgcga tcgccgagac cgtcgtatgg aacttcgtcg cgatcttgta
     2041 cagggtgtcg cccgcgacga ccgtgtacgt caccgctgcg gtcggcgccg cgggaggcgg
     2101 agtcgtggtc gtcggcgtcg ggaccggggg aggcggagtc gtggtcgtcg gcgtcgg
//
LOCUS       DASVKP010000094         4318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_280865, whole genome shotgun sequence.
ACCESSION   DASVKP010000094 DASVKP010000000
VERSION     DASVKP010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280865"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VF362_02050"
     CDS             <1..906
                     /locus_tag="VF362_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7589740.1"
                     /translation="EVAAAGGHYMLMVGPPGAGKTMLAARLPGILPDLAPEDAVLATS
                     VHSIAGTLDPAAGLLTRPPFEAPHHSASVAAIVGGGSHLARPGAISRAHAGVLFLDEA
                     PEFPSSVLQSLRQPLESGEVVLHRAAGAARYPARFQLVMAANPCPCGRFAGTGEGCSC
                     TPMARRRYFGRLSGPLLDRVDLHVDVRAVRRGTDDQGESSRVVAARVAAAREAASVRF
                     AGLRWRSNAAASPGWLREHTPRPVVAVGLRSLDSGALTARGLDRSFRVAWTLADLAGL
                     PAPGVEQFHQAMVLRTRASVMGAAA"
     gene            903..1769
                     /locus_tag="VF362_02055"
     CDS             903..1769
                     /locus_tag="VF362_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX7589741.1"
                     /translation="MTDLDERGLALVTWSAIAEPGDPLAHAIVELLGAAEALDWVRGA
                     ARDPVAATLALAPHAEPTLIDRAVDAVARWVPRLRDGEAERHLDRAARVGARAVPRGA
                     PEWPDVLDDLGVGAPYCLWVRGPRSVREAFARAIAIVGSRSSTAYGEYVAAALSSGIV
                     DEGWTVVSGGAYGIDARVHRAALAAGGVTVAVMAGGVDRLYPQGNADLLARVLETGAV
                     IAEQPPGFPPHRSRFLTRNRIIAASAATVVVEAAHRSGALSTAHHVADPGRPVGAVPG
                     PVTSPLHLVATA"
     gene            1817..2071
                     /locus_tag="VF362_02060"
     CDS             1817..2071
                     /locus_tag="VF362_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589742.1"
                     /translation="MVLPLDASRSESEGPQPRVRARDFATAAERQAIDAVAARGSTIG
                     EVGRAAGMTPAEVRSALGALELAGALRREGDLWMRAPGGR"
     gene            2202..3143
                     /locus_tag="VF362_02065"
     CDS             2202..3143
                     /locus_tag="VF362_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX7589743.1"
                     /translation="MATTVTGRGTTFAAFEDSLRFERGYSGNTVRAYLSDLAHLVAFL
                     RRGDAAAAPEPDALLAGVTLETLRAWLASMSAEGLSRTTLARRGAAARTFFSWAADAE
                     RIPTNPALRLASARPAQSLPLVLSPADMVVMLGVAEAACDDGDPIALRDWAAAEMLYA
                     TGMRVGELAGIDVDDVDLAGRLVRVMGKGAKERVIPFGVPAARAAREWLERGRPALSV
                     SVPGGAAFLVGRKGKRVDQRQIREAVHRLSALSGVTDVAPHALRHTAATHLVSGGSDL
                     RSVQEVLGHASLSTTQRYTHVTAERLRASYKQAHPRA"
     gene            3153..3989
                     /locus_tag="VF362_02070"
     CDS             3153..3989
                     /locus_tag="VF362_02070"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HEX7589744.1"
                     /translation="MSNLNTLLKDHGLKGESMTSASTIPAATTPWWDALAGTDGARAA
                     EARRNLITYYAPLVTQVALRMIVRLPDTVELGDLVSYGMFGLIDAIEKFDPERGYKFE
                     TYAATRVRGAIIDELRAADWVPRSVRSKARQVEAATRALEQQEQCRVTNAAVADRLGW
                     EVGDVRAVRAQVALSYVAPLGGLPRDGSEIEREVSARSLRVPSASQAMDVRETLQLLG
                     QAIRGVREREQEVLRLYYFEDLTLGQIGKILGVTESRVSQIHSCAVAKLRETLVRTGA
                     FD"
     gene            complement(3986..>4318)
                     /locus_tag="VF362_02075"
     CDS             complement(3986..>4318)
                     /locus_tag="VF362_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX7589745.1"
                     /translation="AGHRGIDLRAIEGDAIVSPGEGVVTFVGTVVDRGVVTIDHGGGL
                     VSSIEPITATVAAGDHVDAGDPLGVVSGEPGHCAPRTCVHWGVRFQGEYVNPLDVLAG
                     YGRIVLLP"
BASE COUNT          581 a         1541 c         1561 g          635 t
ORIGIN      
        1 gaggtggccg cagcgggcgg tcactacatg ctcatggtcg gaccgccggg agcgggcaag
       61 accatgctcg cggcccggct tcccgggata ctcccggacc tggcgccgga ggacgccgtg
      121 ctcgcgacct ccgtgcactc catcgcggga acgctcgatc ccgcggcagg gttgctcacg
      181 cgtcctccct tcgaggctcc gcaccactcc gcgtccgtcg ctgcgatcgt cggcggggga
      241 agccatctcg cgaggccggg ggccatctcg cgcgcgcacg ccggggtcct cttccttgac
      301 gaggccccgg agttcccctc gagcgtcctc cagtcacttc gccagccgct tgagtccggc
      361 gaggtcgtgc tccaccgggc cgccggggcc gcgcggtacc ccgcccgatt ccaactcgtg
      421 atggccgcga acccgtgccc gtgcgggcgc ttcgcgggca cgggggaggg gtgctcgtgc
      481 accccgatgg cccgtcgcag gtacttcggg cgcctctccg gcccactgct cgatcgcgtc
      541 gatcttcacg tggacgtgcg ggcggtgcgc cgagggacgg acgaccaggg ggagtcgagc
      601 cgggtcgtcg cggctcgcgt cgccgcggcg cgcgaggcgg cgagcgtgcg cttcgcgggg
      661 ctcaggtggc ggtcgaacgc cgcggcctcg ccggggtggc ttcgcgagca caccccgcgc
      721 cccgtcgtcg cggtggggct tcgatcgctt gacagtggcg cgctcacggc cagggggctc
      781 gatcgatcgt tccgcgttgc gtggaccctc gcggatctcg cggggcttcc cgcgcccggc
      841 gtggagcagt ttcatcaggc gatggtgctt cgcaccaggg cgtcggtcat gggggcggcg
      901 gcgtgacgga tctcgatgaa cgaggtttgg cgctcgtcac ctggagtgcg atcgccgagc
      961 cgggcgaccc attggctcac gccatcgtcg agttgctcgg ggcggcggag gccctcgact
     1021 gggtgcgggg cgccgcgcgc gacccggtcg cggccacact cgccctcgcc ccgcacgccg
     1081 aaccgacgct gattgaccgc gccgtcgacg cggtggcccg atgggttcct cgccttcgcg
     1141 acggcgaagc ggagcggcac ctcgaccgcg cggcgagggt cggggcgcgc gccgtcccga
     1201 ggggggcgcc cgagtggccc gacgtcctcg acgacctcgg cgtcggcgca ccctactgcc
     1261 tgtgggtgcg cggaccccgc tcggtgcgcg aggcgtttgc ccgcgcgatc gccatcgtcg
     1321 ggtcacgctc aagtaccgcc tacggggagt atgttgccgc cgcgctctcc tcgggaatcg
     1381 tggacgaggg atggacggtt gtctctgggg gtgcctatgg gatcgacgcg agggtccacc
     1441 gcgcggccct tgccgcggga ggggtcaccg tggccgtgat ggccgggggc gtggaccggc
     1501 tctacccgca gggcaacgcg gacttgctcg cccgcgtcct ggagaccggc gcggtcatcg
     1561 ccgagcaacc ccccggcttc cctccgcacc gctcgcgctt cctcaccaga aaccggatca
     1621 tcgcagcctc cgccgccacc gtggtggtgg aggccgcgca ccggtcgggc gcgctctcca
     1681 cggcgcacca cgtggcggac ccgggaaggc ccgtcggagc cgtccccgga ccggtgacat
     1741 cgcctcttca tctggttgcc accgcctgat ccgcaacggg gcgatctgcg tcacgtcggc
     1801 cgccgaggtc ctcgagatgg tcctaccgct cgacgcctcc cggtccgaat cggagggtcc
     1861 gcagccccgc gtccgcgcgc gggacttcgc caccgcagcg gagcgccagg ccatcgacgc
     1921 cgtggccgcg aggggctcaa cgatcggcga agtgggccgg gcggcgggca tgaccccagc
     1981 cgaggtgcgg tccgccctcg gcgccctcga gctcgcgggt gcgctccgcc gcgagggcga
     2041 tctgtggatg agagcccccg gcggccgcta atctccgcgg cgcgattatc ggcgcaataa
     2101 cggtagatac caggacaaag agcccttcac cgatgtcatg cgagtgactg atgtgacaaa
     2161 aggggttgat ttgacacctg ggacggtgcc agagtggcgc catggcgacc accgtcacgg
     2221 gtcgcggcac cacgttcgcc gcgttcgagg actccttgcg cttcgagcgc gggtactcgg
     2281 ggaacaccgt gcgcgcctac ctttccgatc tcgcgcacct cgtcgccttc ctgcggcgcg
     2341 gcgacgcggc ggctgccccc gaacccgatg cgctcctcgc gggcgtcacc ctcgagacgc
     2401 tgagggcctg gctcgcctcg atgtccgccg aggggctcag caggaccacg ctcgctcggc
     2461 gcggggccgc ggcgcggacg ttcttctcgt gggccgcaga tgcggagcga atcccgacga
     2521 acccggccct tcgcctcgcg tccgcacgcc ccgcgcagag cctgccgctc gtcctgtcgc
     2581 ccgccgacat ggtcgtcatg ctcggcgtcg cggaggccgc gtgcgacgac ggtgacccca
     2641 ttgccctgcg ggactgggcc gccgccgaga tgctttacgc gacggggatg cgcgtcggcg
     2701 aactcgcggg catcgacgtg gatgacgtag acctggcggg acggctcgtc cgcgtcatgg
     2761 ggaagggcgc caaggagcgg gtcattccct tcggggtccc ggcggcccgc gccgcgcgcg
     2821 aatggcttga gcgggggcgt cccgcgctgt ccgtctcggt cccgggaggc gcggccttcc
     2881 tggtggggcg caagggcaag cgcgtcgacc agcgtcagat ccgcgaggcg gtgcaccggc
     2941 tctcggcgct gtccggggtc accgatgtag cgccccacgc gctgaggcac acggccgcga
     3001 cccacctcgt gtcgggaggg tcggacctcc ggtccgtgca ggaggttctc ggtcacgcga
     3061 gtctcagcac cacccagcgg tacacgcatg tcacggcgga acgccttcgg gcgtcctaca
     3121 agcaagcgca tccgcgcgcc tgagggagca tcatgtccaa cctgaacacc ctgctcaagg
     3181 atcacggact caagggcgag tccatgacct ccgcgagcac gattcccgcg gcaacgacgc
     3241 cgtggtggga cgcactggcg ggcacggacg gcgcgcgggc cgccgaggca cgccggaacc
     3301 tcatcacgta ctacgccccg ctcgtcaccc aggtcgcgct gcgcatgatc gtcaggctgc
     3361 ccgacactgt cgagctcggg gacctggtct cgtacgggat gttcgggctg atcgacgcca
     3421 tcgagaagtt cgacccggag cgcggctaca agttcgagac ctacgcggcg acgcgcgtga
     3481 ggggtgcgat catcgacgag cttcgagccg cggactgggt cccgaggtcg gtgcggagca
     3541 aggcccgtca ggtcgaggcc gcgacgcgcg ccctcgagca gcaggagcag tgccgcgtca
     3601 cgaacgccgc cgtcgccgac aggctcgggt gggaggtcgg cgacgtccgg gcggtgcgcg
     3661 cacaggtggc gctgtcctac gtcgcgccgc tcggcggcct tccgcgggac ggaagcgaga
     3721 tcgagcgtga ggtctccgcg cggtcgttgc gcgtcccgag cgcgagccag gcgatggacg
     3781 tgcgcgagac gttgcagctc cttggccagg caatccgggg ggtcagagaa cgggagcagg
     3841 aggtcctgcg cctgtactac ttcgaggact tgaccctcgg ccagatcggc aagatcctcg
     3901 gcgtcaccga gtcgcgcgta tcccagatcc attcgtgcgc ggtcgccaaa ctgcgcgaga
     3961 cgctcgtgcg cacgggagcc ttcgactagg gcaggaggac gatcctcccg tagccggcga
     4021 ggacgtcgag ggggttgacg tactcgccct ggaaacgcac tccccagtgc acgcacgtcc
     4081 ggggcgcgca gtgcccgggc tctccggaga cgactccgag tgggtctccc gcatccacat
     4141 ggtcgccggc ggccaccgtc gcggtgatcg gctcgatgct cgacacgaga ccgccgccgt
     4201 gatcgatggt caccactccg cgatcgacga ccgtgcccac gaacgtgacg acgccttcgc
     4261 ccggcgacac aatcgcgtct ccctcgatgg cgcgaaggtc gatcccgcga tgccctgc
//
LOCUS       DASVKP010000095         3534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1271366, whole genome shotgun sequence.
ACCESSION   DASVKP010000095 DASVKP010000000
VERSION     DASVKP010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271366"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VF362_02080"
     CDS             <1..513
                     /locus_tag="VF362_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEX7589746.1"
                     /translation="MEDYTYHLEPGKELILGAHMLEVNAALSSKKARLVIAPLGIGGK
                     EDPVRLVFTADAGPAIVVAMSDQRERFRLVANVVDNVPLPAPMPKLPVGHAVWKPRPD
                     FRTSATCWLMSGAAHHTVMSNQIGLDVIREFARMSRIELLAIDESTEVTAFEQELRAN
                     AAYYRLSQGL"
     gene            760..>3534
                     /gene="mfd"
                     /locus_tag="VF362_02085"
     CDS             760..>3534
                     /gene="mfd"
                     /locus_tag="VF362_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012802177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX7589747.1"
                     /translation="MTEDRPLAGVLDVLRDAISPLQSGAASRDIASRIIASRGAFPAL
                     VALASEGARTTLAITATGREAEELATALGAYLDPDSIALFPSWETLPHERLSPRADTV
                     ARRLTTLRRLATPGAEPRIVVAPLRAAIQPMAPGLGAIEPVELRVGERRDLTEVTAAL
                     TRAAYARVDMVERRGEFAVRGGIVDVFAPTDPHPTRVEFFGDEVESIRQFSVADQRSL
                     GESDRLVAPACREMLLTDEVRARAASFAKTAVLAGTTASARDLLARVAEGIAVEGMES
                     LLPVLVDGLVTVVDELPRGSRVVTLEPERLRRRAEDLTKTADEFLHAAWSAATAGGPA
                     PVGVDAGSFRDLDDVRDSAVARGISWASLGPFGGAEALATGIVEPFEQATSPEGAIEA
                     MRARLGEGWKVVIAAAGHGSAERLGEKCGEAELAARVQGWYEPVPGVVSIVPGVMHEG
                     FSAPGASLLVLGERDLTGRAGVAPVRGKTPARRRASVDPIQLRPGDYVVHERHGIGRF
                     VELVTRTVRGIEREYIVIEYSPSRKGMPGDRLSVPTDHLDRVTKYVGGEAPSLSKMGG
                     ADWAKTKGKARRAVREIAAELIRLYSARMATKGHAFGPDTPWQRELEEAFPYVETPDQ
                     LSSIDEVKEDMEREVPMDRLICGDVGFGKTEIAVRAAFKAIQGGTQVALLVPTTLLVR
                     QHADTFAERFAPFPVKVAALSRFQTDKEARLVEEGLADGTIDLVIGTHRLITGQVRFK
                     NLGLVIIDEEQRFGVEHKETLKAMRTSVDVLSMSATPIPRTLEMAVTGIRELSTLATP
                     PEERLPILTYVGPHEDAQIIAAIRRELLRDGQVFYIHNTVRSIDSAAARLRDLVPEAR
                     IGIAHGQMSEHQLESVIVDYYEKRFDVLVCTTIVETGLDIPNANTLVVEKADAFGLSQ
                     LHQLRGRVGRG"
BASE COUNT          530 a         1258 c         1232 g          514 t
ORIGIN      
        1 atggaggact acacgtacca cctcgagccg ggcaaggaac tgatcctcgg cgcccacatg
       61 ctcgaggtca acgcggcgtt gtcgtcgaag aaggcgcgcc tcgtgattgc cccgctcggc
      121 atcggcggaa aggaagatcc cgtgcgcttg gtcttcaccg ccgatgccgg gccggcgatc
      181 gtcgtcgcca tgagcgatca gcgcgagcgc ttccgcctcg tcgccaacgt ggtcgacaac
      241 gtgcccctgc cggcgccgat gccgaagctt cccgtcggcc acgccgtctg gaagccgcgt
      301 cccgacttcc gcacgtcggc gacctgctgg ctgatgtcag gagccgccca ccacacggtc
      361 atgtcgaatc agatcggcct ggacgtgatc cgcgaattcg cgcgcatgtc gcgcatcgaa
      421 ctgctcgcga tcgatgagtc gacggaggta accgccttcg agcaggaact ccgcgcgaac
      481 gcggcgtact accgactctc gcaaggtctt taaccgcccc gccgggcaga tcgacgcgcc
      541 ccgagattgc cagttcgggg cgcgtcggtt cggagcatgg cccgcggatc ggcgccccgc
      601 gtctgtggtg atcgcgggac gccgatccgg gtcccgacgg accccgcgcg tcgtggcgcg
      661 cactagcctg gaggtgccgg caaacgccgg agtcgttcgc gcgcccggag catgtcgcgg
      721 gacgaacctg ggccgcgccg cggggaggag ggtcgggcat tgacggagga tcgaccgctc
      781 gctggcgtgc tcgatgtgct gcgcgacgcg atctcgcctt tgcagagcgg cgccgcctcc
      841 cgggacatcg cctcccggat catcgcctcg cgcggcgcgt tccccgcgct ggtcgcgctc
      901 gcctccgagg gggcccgaac gacgctcgcg atcaccgcga cgggccgaga ggcggaggaa
      961 ctcgcgacag cgctgggcgc gtacctcgac cccgactcga tcgcgctctt cccctcgtgg
     1021 gagacgctcc cgcacgagcg cctctcgccc cgcgccgaca cggtcgcgcg gcgcctcacg
     1081 acccttcgcc gcctcgcgac gccaggggcc gagccgcgga tcgtcgtcgc gccgctccgc
     1141 gcggcgattc agcccatggc gccgggcctc ggcgcgatcg aacccgtgga gctcagggtg
     1201 ggggagcggc gcgacctgac tgaggtgacg gccgccctga cgcgcgccgc ctacgcccgc
     1261 gtcgacatgg tcgagaggcg cggcgagttc gcggttcgcg gcggcatcgt cgacgtcttc
     1321 gcgcccacgg atccccaccc gacccgcgtc gagttcttcg gcgacgaggt cgagtcgatc
     1381 cggcagttct cggtcgcgga ccagcggtcg cttggcgaat ccgatcgact cgtggcgccc
     1441 gcgtgtcgcg agatgcttct cacggacgag gtgcgcgcca gggccgcatc tttcgccaag
     1501 accgcagtcc tcgccgggac caccgcgagc gcccgcgacc tgctcgcgcg ggtcgccgag
     1561 gggatcgcgg ttgaagggat ggagagcctc cttcccgtcc ttgtcgacgg cctggtgacc
     1621 gtcgtcgacg aactgccacg tgggtcgcgg gtcgtcaccc tcgagcccga gcgtcttcgg
     1681 cggcgtgccg aggatctgac gaagactgcg gacgaattcc tccacgccgc atggtcggcc
     1741 gcgacggcgg gaggacccgc gcccgttggc gtcgacgcgg gctccttcag ggacctcgat
     1801 gacgtccggg actctgcggt cgcccggggg atctcctggg cctccctggg gccttttggg
     1861 ggcgcggaag ccctcgccac cggcatcgtc gagcccttcg agcaggccac gtcgcccgag
     1921 ggcgcgatcg aggcgatgcg ggcgcgactc ggcgagggat ggaaggtcgt gatcgccgcg
     1981 gcgggacacg ggagcgccga gcgccttggc gaaaagtgcg gggaggcgga gctcgccgcc
     2041 cgggttcagg gctggtacga acccgtcccg ggggtcgtga gcatcgtgcc aggcgtgatg
     2101 cacgagggct tctccgcccc gggcgcctcc ctcctggtcc tgggcgagcg tgacctgacg
     2161 ggccgcgcag gcgtcgcgcc cgtccgaggc aagacgccgg cccgccgccg cgcctccgtc
     2221 gaccccatcc aactgcgccc gggggactac gtcgtccacg agcgtcacgg catcggccgg
     2281 ttcgtggagc tcgtgacgcg gacggtgcgc gggatcgagc gcgagtacat cgtcatcgag
     2341 tactccccgt cgcggaaggg gatgccgggc gaccgcctct ccgtgccgac cgaccacctc
     2401 gaccgggtca ccaagtacgt cgggggcgag gccccctctc tcagcaagat gggcggcgcg
     2461 gactgggcga agaccaaggg caaggcgcgg cgcgcggttc gcgagatcgc cgccgaactc
     2521 atccgcctgt acagcgcgcg gatggcgacc aagggccacg ccttcgggcc ggacaccccg
     2581 tggcagcgcg agctggagga ggcctttccc tacgtcgaga cgcccgatca actctccagc
     2641 atcgacgagg tcaaggagga catggagcgc gaggtcccca tggaccgcct gatctgcggc
     2701 gacgtcggct tcgggaagac cgagatcgcc gtgcgcgccg cgttcaaggc gattcagggc
     2761 gggacgcagg tcgcgctcct ggtgcccacc acgctcctgg tccgccagca cgccgacacg
     2821 ttcgccgagc gcttcgcccc tttccccgtt aaggtcgccg ccctgtcgcg cttccagacg
     2881 gacaaggagg cgcggctcgt cgaggagggg ctcgccgatg gcacgatcga cctcgtgatc
     2941 gggacccacc ggctgatcac agggcaggtc cgattcaaga acctgggcct cgtgatcatc
     3001 gacgaggaac agcgcttcgg cgtggagcac aaggagaccc tcaaggcgat gcgcacgtcg
     3061 gtggacgtcc tgtcgatgtc ggccacaccc atccctcgca cgctcgaaat ggcggtcacg
     3121 ggaatccgcg aactgtccac cctcgcgacc ccacccgagg aacggcttcc gatcctcacg
     3181 tacgtcggtc cgcacgagga cgcccagatc atcgccgcga tccgccgcga gctcctgcgc
     3241 gacgggcagg tcttctacat tcacaacacc gtccgctcca tcgacagcgc ggccgcgcgc
     3301 ctgagggacc tcgtcccgga ggcgcgcatc gggatcgccc acgggcagat gtcggagcac
     3361 cagctcgaga gcgtgatcgt cgactactac gagaagcgct tcgacgtcct ggtctgcacg
     3421 accatcgtgg agacggggct ggacattccc aacgcgaaca cgctcgtcgt ggagaaggcg
     3481 gacgccttcg ggctgagcca acttcaccaa ttgcgtggtc gcgtcgggcg tggg
//
LOCUS       DASVKP010000096         1741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1435214, whole genome shotgun sequence.
ACCESSION   DASVKP010000096 DASVKP010000000
VERSION     DASVKP010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435214"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VF362_02090"
     CDS             <1..1219
                     /locus_tag="VF362_02090"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX7589748.1"
                     /translation="LVASASRAGRRVGARSSDCDALEAAARNLAAAFDQVRIGRLPFG
                     LPDGLALAVAATRDAARVVLRGVEAAKEGSGAAAKLATARLTEVIDISDALLGDTSPL
                     VVWLAPADGDYGGMVAERILAAPLDVAGLVRSKLLEETAAVFTSATLALGGSFASMAR
                     QLGVDEPVTLDAGSPFDHSSQGILYVASHLDRPGSGGIAADALDELADLISAAGGRTL
                     GLFSSHRAAVEATEAMRSRLDVPIMYQKDDQVATLLARFRADPRACLFGSTTLWQGVD
                     LPGDTCTLVVIDRVPFPRPDDPIAQARSEAVDAAGGSGFMAVSATHAALLLAQGAGRL
                     IRTLSDRGVVAILDSRVATARYGGFLARSLPPLWPTTDRVLVLDALRRLDTERRLDTE
                     RRLDAERTGGVAT"
     gene            complement(1232..>1741)
                     /gene="lexA"
                     /locus_tag="VF362_02095"
     CDS             complement(1232..>1741)
                     /gene="lexA"
                     /locus_tag="VF362_02095"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX7589749.1"
                     /translation="TKGYLRRDANLPRAIDVSMPGDNDADVIDAAASRTRASGNTELP
                     PGVRAVPLVGRIAAGGPVLAEQAVEDVFALPTQLTGEGDLFMLKVAGDSMIDAAICDG
                     DWVVVRRQNVAQDGDVVAALLEDEATIKTFRRRDGHVWLMPHNPAYTPIDGDHAAVMG
                     KVVSVLRRL"
BASE COUNT          218 a          620 c          634 g          269 t
ORIGIN      
        1 gctcgtcgcg tccgcgtcgc gcgccgggcg ccgggtcggc gcgcggagtt cggactgcga
       61 cgcgctcgag gcggccgcgc ggaaccttgc ggcggccttc gaccaggtgc gcatcggcag
      121 gctcccgttc ggcctgcccg acggcctcgc gctcgccgtc gccgcgacgc gcgacgcggc
      181 gagggtcgtt ctccggggcg tcgaggctgc caaggaggga agcggggcgg ctgccaaact
      241 ggcaacggct cggctgacgg aggtgatcga catctccgac gccctgctgg gcgacacgtc
      301 ccccctcgtg gtgtggctgg cccccgccga cggcgactac ggcggcatgg tggccgagcg
      361 aatcctcgcc gcgcccctcg acgtggccgg actcgtgcgc agcaaactcc tcgaggagac
      421 ggcggccgtg ttcacgtcgg ccaccctcgc gctcggcgga tcgttcgctt ccatggcgag
      481 gcagctcggc gtcgacgagc ccgtgacgct cgatgccggg agcccgttcg accactcgtc
      541 gcagggcatc ctctacgtcg cgtcccacct cgatcggccg ggctccggcg gtatcgccgc
      601 ggacgccctc gacgaactcg cggacctgat atcggcggcg ggagggcgga cgctcgggtt
      661 gttctcgtct caccgcgccg cggtcgaggc gacggaggcg atgcgctccc gccttgacgt
      721 gcccatcatg tatcagaagg acgatcaggt cgcgacgctc ctcgcgcgat tccgcgccga
      781 tccgcgcgcg tgcctgttcg ggtcgacgac gctctggcag ggcgtcgacc ttcccggcga
      841 tacctgcacg ctcgtcgtca tcgaccgggt gccgttcccg cggcccgacg acccgatcgc
      901 ccaggcgcgc tccgaggccg tcgacgctgc gggggggagc gggttcatgg ccgtgtccgc
      961 gacccacgct gcgctgctcc tcgcgcaggg cgccggccga ctgatccgga cgctgtcgga
     1021 caggggcgtg gtcgcgatcc tcgactctcg cgtcgcgacg gcgcgctacg ggggattcct
     1081 cgcgcggtcg ttgcccccgc tgtggcccac gacggaccgg gtgctcgtgc tcgacgctct
     1141 gcgtcgtttg gacaccgagc gtcgtttgga caccgagcgg cggctcgacg ccgagcggac
     1201 cggcggggtc gcaacctaga ggcttcgcaa cctagaggcg gcgtagcacc gagacgacct
     1261 tgcccatcac ggcggcgtgg tccccatcga tgggggtgta ggcggggttg tggggcatga
     1321 gccacacatg cccgtccctt cgacggaacg tcttgatcgt cgcctcgtcc tcgagaaggg
     1381 ccgcgacgac gtcgccgtcc tgcgcgacgt tctgtcggcg gacgacgacc cagtcgccgt
     1441 cgcagatcgc ggcgtcgatc atggagtcgc cggcgacctt cagcatgaac agatcgccct
     1501 cgccggtcaa ctgggttggg agcgcgaaga cgtcctcgac ggcctgttcc gcgagcacgg
     1561 ggccaccggc ggcgatgcgc ccgacgaggg gcacggcgcg aaccccagga ggcagttcgg
     1621 tgttgcccga cgccctggtc cgcgaggccg ccgcatcgat cacgtcggca tcgttgtcgc
     1681 cgggcatgct cacgtcgatg gcgcggggga ggttggcgtc ccgccggagg tatcccttgg
     1741 t
//
LOCUS       DASVKP010000097         3118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1354584, whole genome shotgun sequence.
ACCESSION   DASVKP010000097 DASVKP010000000
VERSION     DASVKP010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354584"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VF362_02100"
     CDS             complement(<1..380)
                     /locus_tag="VF362_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589750.1"
                     /translation="MVDDYPAPSRAASDDGAQSSPQVGAGPGESREDRYAMPASPETE
                     LGSTSEVVQDRYVIPEPEEIVPTYVRHEAPWVGFGPSVAGPPPPSEESEFTRILRRRR
                     HGRESVAWVITGAFIVTAAWFLPVL"
     gene            440..1387
                     /gene="dapD"
                     /locus_tag="VF362_02105"
     CDS             440..1387
                     /gene="dapD"
                     /locus_tag="VF362_02105"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX7589751.1"
                     /translation="MTRTAYAFGLATAAEDGTILDTWYPRPMLGEPPASDEVPADLRA
                     GERTDALRGVSLSAIMTRIDCDAPPADVPDAYLRLHLLSHRLVLPHGINLSGIFGVLA
                     NVAWTSLGPCAIASIEQVRTRCAAEGIHLAVHSVDKFPRMVDYVVPSGVRIADADRVR
                     LGAYLAEGTTVMHEGFVNYNAGTLGTSMIEGRVSSGVVIGDGSDVGGGASIMGTLSGG
                     GKEVVSLGRRSLLGANSGLGIPLGDDCVVEAGLYLTAGAKVLLVGQAGDDGAPVVAKA
                     RDLAGQDGLLFRRNSMTGAIEAVRREGSGITLNSALHRN"
     gene            1450..2274
                     /locus_tag="VF362_02110"
     CDS             1450..2274
                     /locus_tag="VF362_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589752.1"
                     /translation="MATFAVIEGPKLFHRYGDGLFASSRCTVTIDGASSTLTAEQADN
                     AALIAATALRRGLPAHAVTVALAAAIQESDLRNLDYGDRDSVGLFQQRPSQGWGTVEQ
                     IRDPRFATNAFYDHLQEVAGWQQIPVMEAAQAVQRSGHPLGYADHEADARLWARALTG
                     ETPFGAVTCSLRSPKADPAAAASFAGRISGDFGSRVSVTIEPESDGATAVRVSSGEQS
                     VLDAAATWSIAVASLWPVADVTLCDLRWDRAGGTLQASEPAASACTPSVVVRFATG"
     gene            complement(2291..2773)
                     /locus_tag="VF362_02115"
     CDS             complement(2291..2773)
                     /locus_tag="VF362_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589753.1"
                     /translation="MSIDFRREYQVAWARCEATRLRTAFGSSIVVVEHAHLEMARGDR
                     LLTISDPGGKGASCAVCVTEHSPEGSSWRIELRPILGSDDADVPATRAIQHLRLQMRY
                     NTCVHATWDETVGARSGEDGSPDLAKGVTTLLRGDFKVHRRGSIGGTRSAQYASVLAA
                     "
     gene            complement(2894..>3118)
                     /locus_tag="VF362_02120"
     CDS             complement(2894..>3118)
                     /locus_tag="VF362_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX7589754.1"
                     /translation="RKLYPNVDFYTGLIYKAMGFPTGMFTPLFALGRVPGWIAHWREM
                     VRDPQTKIGRPRQIYIGETERAYVPMEERP"
BASE COUNT          485 a         1086 c         1057 g          490 t
ORIGIN      
        1 aggacgggaa ggaaccaagc ggccgtgacg atgaacgccc cggtgatcac ccacgcgacc
       61 gactcgcgcc cgtgtctgcg gcggcggaga atgcgcgtga actcggactc ctcgctcggg
      121 gggggcgggc cggcgaccga cggcccgaat cccacccacg gcgcctcgtg gcgaacgtac
      181 gttggcacga tctcctcggg ttcggggatc acgtagcggt cctggacgac ctcgctcgtc
      241 gaacccaact cggtctcagg cgacgcgggc atggcgtatc gatcctcgcg ggactccccc
      301 ggaccggcgc caacctgcgg ggaactctgc gcgccgtcgt cgctagcggc gcgtgagggg
      361 gcggggtagt catcgaccat gggaccaggc tacgccgggc ctgccacgcc gcggcaaggc
      421 gtcgccgtag gctagggcca tgacacggac ggcgtatgca ttcggcctgg ctacggcggc
      481 ggaggacggg actatcctcg acacctggta tccccggccc atgcttggcg agccccccgc
      541 cagcgatgaa gtccctgccg atctccgggc cggtgaacgg accgatgccc tccggggtgt
      601 gagcctcagt gcgatcatga cccgcatcga ctgcgacgcc ccgcccgccg acgttcccga
      661 cgcctacctt cgccttcacc ttctctcgca ccggctggtg ctcccccacg gcatcaacct
      721 gtcgggcatc ttcggagtcc tcgccaacgt ggcatggacg tcgctcggcc cctgcgcgat
      781 cgcgtcgatc gaacaggtcc ggacgcgctg cgccgcagaa ggtatccacc tcgcggtgca
      841 ctccgtcgac aagttccccc gcatggtgga ctacgtcgtc ccgtcgggcg tgagaattgc
      901 ggacgccgac cgggtgcgcc tgggcgcgta ccttgccgag ggcacgacgg tcatgcacga
      961 aggcttcgtc aactacaacg cgggcaccct cgggacgagc atgatcgaag ggcgtgtgtc
     1021 atccggcgtc gtcatcggcg acggctccga cgtcggcggt ggcgcctcga tcatgggcac
     1081 cctctcgggc ggcggcaagg aggtcgtctc gctcgggcgg cgctcgctgc tcggggcgaa
     1141 ctccggcctc gggattccgc tcggggatga ctgcgtcgtc gaggcgggcc tctacctcac
     1201 ggcgggagcc aaggtcctcc ttgttggtca ggcgggggac gacggcgctc ccgtcgttgc
     1261 gaaggcgcgc gacctcgcgg gccaagatgg gctcctcttc cgtcgcaact ccatgaccgg
     1321 cgccatcgag gcggttcggc gcgagggctc ggggatcacc ctcaattccg ccctgcaccg
     1381 caactgatgg cccgccggag gcgcaggaga cccctcgctg gctgtgcggg cctactggtc
     1441 ttcgcagcgg tggcgacctt cgcggtcatc gaggggccga agttgttcca ccgctacgga
     1501 gacggactct tcgcctcgtc gaggtgcacg gttacgatcg atggcgcatc gtcgacgctc
     1561 acggccgagc aggccgacaa cgcggcgctt atcgccgcga cggcccttcg gcgagggctc
     1621 cccgcccacg cggtgaccgt ggccctcgcg gcggcgatac aggagtcgga cctcaggaac
     1681 ctcgactacg gcgaccgcga ctcggtcggg ctcttccagc agcggccgtc acagggttgg
     1741 ggaacggtcg agcaaatccg cgacccgcgc ttcgccacga acgctttcta cgaccatctg
     1801 caagaggtgg ccgggtggca gcagatcccg gtgatggagg ccgcccaggc ggtccagcgg
     1861 tcgggacatc ccctcgggta cgccgaccac gaggccgacg cgcggctgtg ggcgcgcgcg
     1921 ctcacgggcg agaccccctt cggcgccgtc acctgctcgc tccgctcacc gaaggccgat
     1981 cccgcggcgg ccgcatcatt cgcgggccgc atttccggag acttcggctc ccgcgtatcc
     2041 gtaacgatcg aaccggagtc ggacggcgcc accgcagtgc gggtctcctc tggcgagcag
     2101 tcggtgctgg acgccgcggc aacatggtcc atcgcggtgg cgtccctatg gcctgtcgct
     2161 gacgtcacgc tatgcgacct ccggtgggat cgcgccggcg ggacattgca ggcctccgaa
     2221 ccggccgcgt cggcgtgcac cccttccgtc gtcgtccgat ttgcgacggg gtgattgggt
     2281 tgccggggaa ctacgcggcg agaaccgacg cgtattgggc ggagcgtgtc ccgccgatgg
     2341 atcctcgccg atggaccttg aagtcgccgc gcaggagcgt ggtcacgccc tttgcaaggt
     2401 ccggtgaacc atcttccccg ctccgcgccc ccaccgtttc atcccacgtg gcatgaacgc
     2461 aggtgttgta gcgcatctga agccgaaggt gctggatcgc ccgcgtcgcc ggaacgtcgg
     2521 cgtcgtcgga cccgaggatg ggccggagct caatgcgcca ggaactaccc tcgggcgaat
     2581 gctcggtaac gcagaccgcg cacgatgcgc ccttgcctcc ggggtcggag atggtcagga
     2641 ggcggtcgcc ccgtgccatc tcgagatggg catgctccac gaccacgatg gaacttccga
     2701 aggcggtccg taggcgggtc gcctcacacc gcgcccacgc cacttgatac tctcgacgga
     2761 aatcgatgga catcgccgct ccccgaaata ccccctgccc gccagattag gccccagaat
     2821 cgggacgcgc gaggcgaggg gctgagagat gcctcacacc ccgaagcggg tcgacgagta
     2881 gggacccctc caactatggg cgctcctcca tcggaacgta ggcgcgctcg gtctctccga
     2941 tgtagatctg gcggggacga ccaatcttcg tctgcggatc gcgaaccatc tcgcgccagt
     3001 gagcgatcca gcccggcacg cgaccaaggg cgaagagcgg cgtgaacatg cccgtcggga
     3061 accccatggc cttgtagatc aggcccgtgt agaagtccac gttcggatag agcttgcg
//
LOCUS       DASVKP010000098         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_778089, whole genome shotgun sequence.
ACCESSION   DASVKP010000098 DASVKP010000000
VERSION     DASVKP010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778089"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VF362_02125"
     CDS             complement(<1..494)
                     /locus_tag="VF362_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX7589755.1"
                     /translation="MSAMRLSPRRHAARGRGHPGLRLLAYAAAAVLGFGSVGSYVYVQ
                     SALNQVHTVDVTALLGDQLPVVPVAPTDGAKGEWINILLMGSDSRSGANAAIGGEVGG
                     MRNDTTIIMHISADRSRIEFVSIPRDSWVRISDCKLFDGSTVKGWTGKFNVAFANGAK
                     HHNSA"
     gene            complement(618..1817)
                     /locus_tag="VF362_02130"
     CDS             complement(618..1817)
                     /locus_tag="VF362_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX7589756.1"
                     /translation="MRNVLRVRPRRHAARGPGHPFVRGLAYLAAAIVGFAGVGTYEYI
                     QTAFDRVHTVDVTALLGDQRPVAPVAPADASKGLPINILLMGSDSRAGVNASIGGAND
                     GMRSDTTIILHVSADRTRIEFVSIPRDSWVKISDCTLFDGTVVKGRTTKFNAAFANGA
                     KHNDPAEAAACAMKTTEDLTGLFIDYYAVVDFAGFAAMVDSLGGVPMCVPGRIVSAES
                     GLNVQAGPQLFAGSTAIAWARTRKVEIGKQYYDGTDIKRMKAQQVLLSHMVETALSKN
                     LLWNGGQIRDFVTSAADSLTVSPTLANPEFVIGLAFSLRHISKDSIVFTTIPFTWTPD
                     GNNVTWNADGYALLDNIRNDRPIAGTSVTDASFAAPVAVPSPSSSVGPGATPQPSMDP
                     LGACPPG"
BASE COUNT          338 a          646 c          745 g          365 t
ORIGIN      
        1 gccgagttgt gatgcttcgc gccgttggcg aacgctacgt tgaacttccc cgtccacccc
       61 ttcaccgtcg acccatcgaa taacttgcag tcggagatgc gcacccagga gtctcgcggg
      121 atcgagacga attcgatgcg cgatcggtcc gcggagatgt gcatgatgat cgtcgtgtcg
      181 ttgcgcatgc cgccgacctc gccaccgatg gccgcgttgg ccccggagcg agagtcggag
      241 cccatcaaca ggatattgat ccactcgccc ttggcgccgt ccgtcggagc gaccggaacg
      301 accgggagct gatcgccgag gagcgcggtc acgtcgacgg tgtgcacctg attcagggcg
      361 ctctgcacgt agacgtagct tcccaccgag ccgaagccga gcacggcggc tgcggcgtag
      421 gccaagagcc gaagtcccgg gtgacccctt ccccgggccg cgtggcgacg cggggaaagg
      481 cgcatggcgc tcatgagcgt cctttcctcc cggtccgtcg gggctcgtcg cttcctggta
      541 gcaccaacgc ttcccgcgaa ggattcgttg cccgcgcagt atcgtacggc cccgtgcgag
      601 ccttcccgca cgccgcccta accgggcggg caggccccga gggggtccat gctcggctga
      661 ggggtcgctc cggggccgac ggaggacgac ggggacggga ccgcgaccgg ggcggcgaag
      721 ctggcatccg tgacggaggt gcccgcgatc gggcggtcgt tacggatgtt gtcgaggagg
      781 gcgtacccgt ctgcgttcca ggtcacgttg ttcccgtcag gggtccaggt gaaggggatc
      841 gtggtgaaga cgatgctgtc cttcgatatg tgccgaaggc tgaaggcgag cccaatgacg
      901 aactccgggt tcgcgagggt cgggctcacg gtgagcgaat ccgccgccga cgtgacgaag
      961 tcgcgtatct ggccgccgtt ccacagcagg ttcttggaca gagccgtctc caccatgtgg
     1021 gacagcagca cctgctgtgc cttcatgcgc ttgatgtcgg ttccgtcgta gtactgcttg
     1081 ccgatctcga ccttcctggt cctcgcccag gcgatcgccg tcgacccggc gaagagttgc
     1141 gggccggcct ggacattgag cccggactcg gccgacacga tcctcccggg gacgcacatg
     1201 ggcacgccgc cgagcgagtc gaccatggcc gcgaatccag cgaagtcgac gacggcgtag
     1261 tagtcgatga agaggccggt gagatcctcg gtggtcttca tggcgcacgc ggcggcctca
     1321 gccggatcgt tgtgcttcgc gccgttggcg aaggcggcgt tgaacttagt ggtgcgtccc
     1381 ttgacaacgg tcccgtcgaa gagggtgcaa tccgagatct tgacccacga gtcgcggggg
     1441 atggagacga actcgatcct ggtgcggtct gcggagacgt gcaggatgat cgtcgtgtcg
     1501 gaccgcatgc cgtcgttcgc gccgccgatg gaggcattga cgccggcccg cgaatcggaa
     1561 cccatgagga ggatgttgat cggtagcccc ttggaggcgt cggccggggc gaccggggcg
     1621 acggggcgct ggtcaccgag gagcgcggtc acgtcgaccg tgtgaacgcg atcgaacgcg
     1681 gtctggatgt actcataggt tccgacgccc gcgaacccga cgattgcggc ggcgagatac
     1741 gcgaggccgc gcacgaacgg gtggccgggt ccccgcgccg cgtggcggcg cgggcggact
     1801 cggagcacgt tcctcacggt cgtccttccc ggtggcgggg gagagggtgg atggagtctt
     1861 caacgttcag gcgacaccta ttgtcgcaga gcgtcggttc ggtcgcggcg aaacacgggg
     1921 cgtcctcgcg gtgttttctc gctttgggtc taccctctgc gctcgcggtg gggtaccgtc
     1981 gacttgacgg gcggggatta caccgatgta attccgaata gcggtgagaa ctccgcgcaa
     2041 accggaggag ggccacatgt ggcacgtcta cgacggacac attccgttgg acgc
//
LOCUS       DASVKP010000099         3620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1025199, whole genome shotgun sequence.
ACCESSION   DASVKP010000099 DASVKP010000000
VERSION     DASVKP010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025199"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /gene="folK"
                     /locus_tag="VF362_02135"
     CDS             <1..352
                     /gene="folK"
                     /locus_tag="VF362_02135"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX7589757.1"
                     /translation="VHGRERAVVNGPRTLDIDIITYDDVVAAAEDLVVPHPRAHERSF
                     VLSPWGALEPDAVLLAGPGRNPTRGGRVVELAVLAPDAAGMMVVASPWDPIAAIRSRA
                     AGSPPPPPPPPPTG"
     gene            355..840
                     /locus_tag="VF362_02140"
     CDS             355..840
                     /locus_tag="VF362_02140"
                     /inference="COORDINATES: protein motif:HMM:NF022819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3180 domain-containing protein"
                     /protein_id="HEX7589758.1"
                     /translation="MARLTWRRVTLVAAAVSVIGWLVATLILRSGASPAPVPWTVPPV
                     LLVAGVLALRLGWRVKKFVDGKRPGLDPLQAARTAMFAQASAYSGAALAGAYLGYAVA
                     LLPDWSHQPRREVIIAAFVAMAAAALLCAAGWLAEHWCAAGGGDKPPRPSGSTAGVAP
                     G"
     gene            853..1332
                     /locus_tag="VF362_02145"
     CDS             853..1332
                     /locus_tag="VF362_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX7589759.1"
                     /translation="MSWFEPAGVTWTYASRRLIAARLIVGIVPVALLGAAVGAMASLL
                     GAHPAASVGCGLAVVAWWAWVVVRQVTAHGWVERQDDLMVKRGRIFRRVTVVPYGRMQ
                     YVEVNAGPVARAFSLARVQLHTASPGTDAHLSGVPAEEAARLRDRLTVRGEARLAGL"
     gene            1329..2924
                     /locus_tag="VF362_02150"
     CDS             1329..2924
                     /locus_tag="VF362_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX7589760.1"
                     /translation="MTSQSFAPPEEWTRLHIVTPVLLAGRAAGVFVVVALAQGGQGGL
                     LAAKNAPAWVPLAGIVIVIAVASAIAFGEWWMNQFRVDDDAVHQRKGILFRQYRQARL
                     DRLQAVDVVQPLVARVFGFAEVKIDVAGGKASSVRLQYLRLEDAQALRNEIVALAAGI
                     GRAPVGASVATAPPREKAGPALSGEPILAPVSHTPALPAPASQAPVLHASARREPVGA
                     APQREVYAVPPGRLVGSIVASWWTLFIVGFALVTIIVVVVMGAVAVRKFAGDAISSGG
                     GIEGFLSGGAYGVLSGLFAFFAVGWARFSSGFGFTAAISADGIRLQHGLLDTRHQTVP
                     PGRVQAVRLRQGLPWRRFDWWRITINVAGYQDEEQAETTLLPVGPRRDALYALWLVLP
                     DLGDPDPAGTVSAALSGVGADNGFTATPRRAWLVDPLQWRRRGVLATGRALLIRQGLF
                     ARDLVVVPHEKTQSLSIRQGPLQRLLSLASVEVHSTKGSISPVAHHLDVADAIALVDA
                     QADRAREARGRQSPEQWMATVQR"
     gene            2938..>3620
                     /locus_tag="VF362_02155"
     CDS             2938..>3620
                     /locus_tag="VF362_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX7589761.1"
                     /translation="MRPARLDVGVVGGGRVGAVLGAALRLAEHRVVGASGISEASATR
                     IDALLPGTPRLDPEAVVRAADLILLTVPDDALAAVVAGLASVGAWRPGQIAVHASGLH
                     GLAVLEPAARLGVTTLAIHPAMTFTGTSIDLARIREAVFAVTAAAPVLPIALALVVEL
                     GGEPVVVADEDRALYHAGVVHGANHVVALVTQAMDALRAAGVEDPGRLIGPLVRASID
                     GALSEAPGTA"
BASE COUNT          421 a         1291 c         1320 g          588 t
ORIGIN      
        1 ggtccacggg cgcgagcgcg cggtcgtcaa cgggccgcgc accctcgaca tcgacatcat
       61 cacgtacgac gacgtcgtcg ccgccgcgga ggacctggtc gttccgcacc cgcgcgccca
      121 cgagcgttcg ttcgtgctct ccccgtgggg agcgctcgag ccggatgccg tgcttctcgc
      181 gggccccgga cgcaatccca ctcgaggagg aagggtcgtc gaactcgcgg tcctggcgcc
      241 ggatgccgcg ggaatgatgg tcgtcgcgag tccgtgggat ccgatagcgg cgatccggtc
      301 gcgcgccgct ggctcaccgc cgccgcctcc gccccccccg ccaacgggct agcggtggcg
      361 cgcctcacct ggcggcgcgt gacgctcgtt gccgcggccg tctcggtcat cgggtggctc
      421 gtggcgacgc tgattcttcg atcgggcgct tcccccgcgc ccgtgccgtg gaccgtgccg
      481 ccggtgcttc tcgtggcggg cgtcctcgcg cttcggctcg gctggagggt caagaagttc
      541 gtcgatggca agcgtcccgg cctcgacccc cttcaggccg cgcgcacggc gatgttcgcc
      601 caggcctccg cctactccgg ggcggccctc gcaggggcct acctcggcta cgccgttgcg
      661 ctcctgccgg actggtccca tcagccccgg cgagaggtga tcatcgcggc gttcgtcgcg
      721 atggcggccg cggccctcct gtgcgccgcg gggtggctcg cggagcattg gtgcgccgca
      781 ggcggcggcg acaagccccc gaggccgtcc ggttcgacgg ccggagttgc tccggggtga
      841 cagactagcg acatgtcgtg gtttgaacct gcgggtgtca cgtggaccta cgcgtcgcga
      901 cgtttgatcg ccgcgcgcct catcgtcgga atcgttcccg ttgcccttct tggagcggcc
      961 gtcggcgcca tggcgtcgct cctcggcgcg cacccggcgg cctcggttgg ttgcgggctc
     1021 gccgtcgtcg cgtggtgggc gtgggtggtg gttcgccagg taacggcgca cgggtgggtc
     1081 gagcgtcagg atgacttgat ggtcaagcgc ggccgcatct tccggcgcgt gaccgtcgtt
     1141 ccctacgggc gcatgcagta cgtcgaggtg aacgccggac ccgtggcgcg ggccttctct
     1201 ctcgcgaggg ttcaactcca cacggcctct ccggggaccg acgcccacct ctcgggggtc
     1261 cccgcagagg aggccgcgcg cttacgggat cggctgacgg tccgcggcga ggcgcgtttg
     1321 gcgggcctgt gacgtcccag tcgttcgcgc ctccggagga gtggacacgc ctgcacatcg
     1381 tgacgcccgt gctgcttgcc ggacgcgcgg cgggggtctt cgttgtcgtc gccctggcgc
     1441 agggcggcca gggcggcctg ctcgcggcga agaacgcgcc ggcctgggtg cccctcgccg
     1501 ggatcgtgat cgtgatcgcg gtggcatcgg cgattgcctt cggcgagtgg tggatgaacc
     1561 aattccgtgt tgacgacgac gcagttcacc agcgcaaggg aatcctcttc cggcagtacc
     1621 ggcaggcgag gctcgaccgc cttcaggcgg tggacgtggt tcagccgctc gtggcaaggg
     1681 tgttcgggtt cgccgaggtg aagatcgacg ttgcgggagg caaggcgtcg agcgtccgcc
     1741 ttcagtacct gcgcctcgaa gacgcgcagg ccctgcgcaa cgagattgtc gccctggcgg
     1801 cgggaatcgg gcgcgcgccc gtcggcgctt cggtcgccac ggcgccccca cgtgagaagg
     1861 cgggtcccgc gctctccggc gagccgatcc tcgcacccgt atcgcacacc cccgcgctcc
     1921 ccgcacccgc gtcgcaagcg cccgtgctac acgcatcggc gcggcgagaa cccgtcggcg
     1981 ccgcgcctca gcgcgaggtc tacgcggtgc cgcccgggcg actcgtcggg tcgatcgtgg
     2041 cctcgtggtg gacgctcttc atcgtcggct tcgccctggt caccatcatc gtcgtcgtcg
     2101 tgatgggcgc ggtcgcggtg aggaagttcg cgggggatgc catctcctcg ggcgggggaa
     2161 tcgagggctt cctgagcggt ggggcgtacg gcgtgctctc gggcctcttc gcgttcttcg
     2221 cggtcgggtg ggcccgcttc tcctccgggt tcgggttcac ggcggcgatc tccgccgacg
     2281 gcattcggct tcaacacggc cttctcgaca cgcggcacca gaccgtgcct cccggcaggg
     2341 ttcaggcggt ccggctgcgt caagggctcc catggcggcg cttcgactgg tggcgcatca
     2401 cgatcaacgt cgcgggctac caggacgagg agcaggccga aacgacgctc ctgcccgtcg
     2461 gcccgcggcg cgacgcgctc tacgccctgt ggctcgtgct ccccgacctt ggcgatcccg
     2521 acccggcagg gaccgtctcc gcggccctct cgggggtggg ggccgacaac gggttcaccg
     2581 cgacgccccg gcgcgcctgg cttgtcgatc ccctgcagtg gcgccgacgc ggcgtgctcg
     2641 cgacgggccg ggcactgctg atccggcagg gactgttcgc tcgggacctg gtcgtcgtgc
     2701 cccacgagaa gacccagtcg ctatcgatcc gccagggccc cctccagcgc ctcttgtccc
     2761 ttgccagcgt cgaggtgcat tcgaccaagg gctccatcag ccccgtcgcg catcacctcg
     2821 acgttgcgga cgcgatcgcg ctggtggacg cccaggcgga tagggcgcgc gaggcgcggg
     2881 gcaggcagag cccggagcaa tggatggcga cggtgcagcg atgaaaccag ggcagcggtg
     2941 aggccggcgc gcctcgacgt aggggtcgtg ggcgggggcc gggtcggcgc cgtcctcggg
     3001 gcggcgttgc ggctcgcgga gcacagggtc gtcggcgcct cgggcatctc ggaggcgagc
     3061 gccacccgga tcgacgcgct cctgccgggg actccgcgcc tcgaccccga ggcggtggtc
     3121 cgcgccgccg acctgatcct gctcaccgtt cccgacgatg ccctcgccgc cgtggtcgcg
     3181 ggtctcgcgt cggtgggggc atggcggcca ggccagatcg cggtccatgc gtcgggactg
     3241 cacggcctcg cggtgctcga accggccgcg cgcctcggcg tcacgacgct cgcgattcac
     3301 cccgcgatga ccttcacggg aacgtcgatc gatcttgccc ggatccgcga ggccgtcttc
     3361 gccgtcaccg ccgccgcccc tgtactcccg attgccttgg ccctggtcgt ggaattgggg
     3421 ggcgaacccg tcgtcgtcgc cgacgaggat cgcgcgctgt atcacgcggg ggtcgtccac
     3481 ggcgcgaacc acgtggtcgc cctcgtcaca caagcgatgg acgcgctacg cgcggcgggc
     3541 gtggaggatc cgggaaggct catcggcccc ctcgtccgcg cgtcgatcga cggggcgctc
     3601 tcggaggcgc ccggcaccgc
//
LOCUS       DASVKP010000100         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_201375, whole genome shotgun sequence.
ACCESSION   DASVKP010000100 DASVKP010000000
VERSION     DASVKP010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201375"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VF362_02160"
     CDS             <1..837
                     /locus_tag="VF362_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7589762.1"
                     /translation="ALTGSSAMLAESIHSVADSGNQVLLLVGGRRARREATEEHPFGF
                     SRARYYYAFLVAVVLFSVGGLYALYEAWQKFGQPEAIKAWRWVPLVVLGVAMVLESLS
                     LRTAVRESNRVRGTLGWAGFIRGSRSPELPVILLEDVAALLGLVFAFVGVSLTLATGD
                     GRWDAGGTAAIGVLLVTVALVLGRETKSMLLGESATPEDVRRIRAALETPDLGGVIHL
                     RTMHLGPDDVLVAAKVAFPGETSVDTVAALVDAAEARIRAAVPAARLIYIETGVQRRD
                     SP"
     gene            834..>1680
                     /locus_tag="VF362_02165"
     CDS             834..>1680
                     /locus_tag="VF362_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX7589763.1"
                     /translation="MTKPNFVVADLTLASAGRHQIDLAEHEMPGLMAIREEFAATRPL
                     TGARIAGSLHMTVQTAVLIETLVALGADVRWASCNVFSTQDEAAAAVALGSGRLDAPA
                     GVPVFAVKGESLEEYWDYTDRILTWPGEDGPTLILDDGGDATVLVHEGVKAEAAGAVA
                     PRLEPEDRGYHRETEAMRAVLRRSLAADPTRFTRMAAGIVGVSEETTTGVHRLARLHE
                     RGELLFAGINVNDSVTKSKFDNRYGIRHSLPDGLNRGTDVLIGGKVAFVAGYGDVGKG
                     AAEALR"
BASE COUNT          229 a          591 c          593 g          267 t
ORIGIN      
        1 gcgttgacgg gttcgagcgc catgctcgcg gagtccattc actcggtcgc ggattccggg
       61 aatcaggttc tcctgctcgt cggcggacgg cgcgcgcggc gggaggcgac ggaggagcac
      121 cccttcggct tctcgcgggc caggtactac tacgccttcc tcgtcgccgt cgtcctcttc
      181 agcgtcggcg gcctgtacgc gctctacgag gcctggcaga agttcggcca acccgaggcg
      241 atcaaggctt ggcgctgggt gccgctcgtg gttctcggcg tggccatggt cctcgagtca
      301 ctctcgctca ggactgcggt tcgggaatcg aaccgcgtca ggggcaccct cgggtgggcg
      361 gggttcatcc ggggatcccg cagccccgag ttgccggtca tccttctgga ggacgtcgcg
      421 gcgctcctgg gactcgtctt cgccttcgtg ggagtgagcc tcacgctggc aaccggcgac
      481 gggcgctggg acgcgggcgg gacggccgcg atcggcgtcc tgctcgtgac ggtcgccctt
      541 gtcctcggcc gcgagacaaa gtcgatgctt ctcggtgagt cggcgacgcc cgaggacgtc
      601 cggcgcatcc gagctgccct cgagactcct gacctcgggg gcgtcatcca cctccggacc
      661 atgcacctgg ggcccgacga cgtgctcgtc gcggccaagg tcgcgttccc tggcgagacg
      721 tcggtggaca cggtcgcggc gctcgtcgac gcggcggaag cgcgcatccg tgcggcggtt
      781 cccgccgcgc ggctcatcta catcgagacc ggtgtacaac ggagggactc cccgtgacca
      841 agcccaactt cgtcgtcgcc gacctcaccc tcgcctccgc ggggcgacac cagatcgacc
      901 tggccgagca cgagatgccc ggcctcatgg cgatccgtga ggagttcgcc gcgacgcgcc
      961 ccctgacggg tgcgcgcatc gcgggttcgc tccacatgac ggtccagacg gccgtcctga
     1021 tcgagacgct cgtcgcgctg ggtgcggacg tccgctgggc atcgtgcaac gtgttctcca
     1081 cccaggacga ggctgcggcg gccgtcgcgc tcggctccgg gcgcctcgac gcccccgcgg
     1141 gcgttccggt cttcgcggtc aagggcgaga gcctcgagga gtactgggac tacacggacc
     1201 ggatcctcac ctggcccggc gaggacggtc cgacgctcat cctcgacgac gggggcgacg
     1261 cgaccgtact ggtgcacgag ggcgtcaagg ccgaggccgc gggtgccgtc gccccccggc
     1321 tcgagcccga ggatcgcggc taccaccgtg agacggaggc catgcgcgcg gtcctccgtc
     1381 gctccctggc cgcggacccg acgcgcttca ctcgcatggc cgccggaatc gtcggcgtga
     1441 gcgaagagac gacgacgggc gttcaccgcc tcgcgcggct tcacgagcgc ggcgaactcc
     1501 tcttcgccgg gatcaacgtc aacgactccg tcaccaagtc caagttcgac aatcggtacg
     1561 gaattcggca ctcgttgccc gacgggctca accgcggcac cgacgtcctg atcggcggca
     1621 aggtcgcgtt cgtggcgggc tatggggacg tcgggaaggg cgcggccgag gcgctgcgcg
//
LOCUS       DASVKP010000101         3837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_284160, whole genome shotgun sequence.
ACCESSION   DASVKP010000101 DASVKP010000000
VERSION     DASVKP010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284160"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1041
                     /locus_tag="VF362_02170"
     CDS             85..1041
                     /locus_tag="VF362_02170"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX7589764.1"
                     /translation="MSAQDPPRVIAVRAPAKVNLQLAVGPRGDDGYHPLATVFQAVAL
                     WETVVATPRGDGEFTVTMDVQPTIRVPEGSVPLDETNLAVRAARAVANRYGVTGGVDL
                     RIIKGVPVAGGMAGGSADAAAALVACSEALGVGATRGELDELAADLGSDVPFALHGHT
                     AVGQGRGDRLTPAMARGEYHWVFAVQAEGLSTPAVYAEFDRQVAEGERLAMSPVLDEE
                     LMMGLRAGDAATVGARLSNDLEGAALRLAPHLAEVLAAAREAGALGAMVSGSGPTVAA
                     LARSRQHGLAIAAAIAAVDVADGVVVASGPAAGAAVVSAEVL"
     gene            complement(1076..1684)
                     /locus_tag="VF362_02175"
     CDS             complement(1076..1684)
                     /locus_tag="VF362_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7589765.1"
                     /translation="MTAHERREQLLVVGRSLFAERGFDGTSVEEIAARADVSKPVVYE
                     HFGGKEGLYAVIVDREVRALLGRLTEALSRRGHPKQHVQRAALALLGYIEDSPDGFRI
                     LVRDSPVAQATGTFSSLIGDVATQVEGLLAAQFAKRGLDASVAPIYSQMLVGMVALTG
                     QHWSDVREPSKQVVAAHLVNLAWNGLSDLERRPVLDSSGGAA"
     gene            1778..1849
                     /locus_tag="VF362_02180"
     tRNA            1778..1849
                     /locus_tag="VF362_02180"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1810..1812,aa:Gln,seq:ttg)
     gene            1932..3452
                     /gene="glmU"
                     /locus_tag="VF362_02185"
     CDS             1932..3452
                     /gene="glmU"
                     /locus_tag="VF362_02185"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX7589766.1"
                     /translation="MSDERPAAVIVLAAGAGTRMKSAIPKVLHRIGGRTLLHHVLAAA
                     ADTQPGRIVVVVRHEAELVSAHVREVAPAALIADQDEIPGTGRAVWGALVALDAAAKV
                     TGTLLVTSGDVPLMDGGTLSALAASHRADGSAVTVLTTIVTDPTGYGRIIRDPETGDV
                     ARIVEEGDATEGERQITEINTGTYAFDAGALRSALAGIGRANTQGEMYLTDVIAAARA
                     SGGVVRAIMIEDSAAAEGINDRVQLSRAGAELNARILERWMLDGVTIVDPATTWIDAG
                     VTLAADVTLLPGTHLHGTTAVAKLATIGPDTTLTDVIVGEGATIVRSHGTGSVIGAGA
                     SVGPFSYLRPGTNLGRDGKIGAFVETKNAQIGRGSKVPHLSYVGDATVGEESNIGAAT
                     IFVNYDGVTKHHTTVGSHVRIGSDNTLIAPVTIGDGAYTGAGAIIRHDVPAGALAMNS
                     APQQTTEGWVERRRPGTPSARAAEAARDAASKPEALTPEAHNERAAARRSGAKGES"
     gene            3449..>3837
                     /gene="prs"
                     /locus_tag="VF362_02190"
     CDS             3449..>3837
                     /gene="prs"
                     /locus_tag="VF362_02190"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX7589767.1"
                     /translation="MSAGISNANERRLVLVSGRAHPELALQVAEGLGTELLPTDAYSF
                     ANGELYIRFGESVRGADAFVLQSHAAPINKAIMEQLIMVDALKRASAARITVVAPCYG
                     YARQDKKHRGREPISARLMADLFKTAGA"
BASE COUNT          586 a         1320 c         1385 g          546 t
ORIGIN      
        1 accctcgaca tcgctgcgtt cgcgcggctc acggaggcgc tccacgactt ccgtgtgggc
       61 gcctgatctg gcacggtggt gggcatgagc gcccaggacc cgccccgagt catcgccgtg
      121 agggcgcccg cgaaggtgaa ccttcagctc gcggtgggtc cccgcggcga tgacggctac
      181 caccccctgg cgacggtctt ccaggcggtg gcgctgtggg agaccgtggt cgccaccccg
      241 aggggcgacg gcgagtttac ggtcacgatg gacgtccagc cgaccatccg cgtgcccgag
      301 ggctccgttc ccctcgacga aacgaacctc gcggtgcgcg cggcgcgcgc tgtcgcgaac
      361 cgctacggcg tcacgggcgg agtggactta cggatcatca agggcgtccc cgtcgcgggg
      421 ggcatggccg gggggagcgc ggacgccgcg gcggccctcg tcgcgtgctc cgaggcgctt
      481 ggggtcggcg cgacccgagg cgaactcgac gaactcgcgg cggaccttgg ttcggacgtg
      541 ccgttcgcgc tccatggcca cacggccgtc ggccaaggcc ggggcgatcg cctcaccccc
      601 gccatggcgc gaggggagta ccactgggtg ttcgccgtgc aggcggaagg cctctcgacg
      661 ccggcggtct atgcggagtt cgatcgccag gtggccgagg gcgagcgcct cgcgatgtcg
      721 ccggtgcttg acgaggaact gatgatgggc ctcagggcgg gcgatgcggc caccgtcggt
      781 gcgcgcctct cgaatgacct cgagggcgcg gccctccgcc tcgcgcctca cctcgccgag
      841 gtcctcgccg ccgcgcgcga ggcgggggct ctcggggcga tggtctctgg ctccggccct
      901 acggtcgcgg cgctggcccg ctcgcgtcag cacgggctcg ccatcgccgc ggccatcgcg
      961 gcagtggatg tcgccgacgg agtggtggtc gcgtcgggcc ccgcggccgg ggcggccgtg
     1021 gtctcggccg aggtcctcta gggaactcag ggccgccgga cgtctggaag ggtacttagg
     1081 cggccccgcc ggaggaatcg aggaccgggc ggcgctcaag atccgatagg ccgttccacg
     1141 cgaggttcac caggtgcgcc gcgacgacct gcttcgacgg ctcccgaacg tcggaccagt
     1201 gttggccggt cagcgcgacc atgccgacga gcatctgcga gtagatcggg gcgacggagg
     1261 cgtcgagccc gcgcttggcg aactgcgccg cgaggagacc ctccacctgc gtggcgacgt
     1321 cgccgatgag gctcgagaag gtccccgtgg cctgggccac gggcgaatcg cgtacaagga
     1381 tgcggaaccc gtcgggggaa tcctcgatgt agccgagaag ggccagcgcg gcccgctgga
     1441 cgtgctgctt ggggtgtccc cgtcgggaca gggcttccgt cagcctgccg agcagcgccc
     1501 gcacctcgcg gtcgacgatg accgcgtaca gaccctcctt gccgccgaag tgctcataca
     1561 ccacaggctt cgagacatcc gcgcgcgcgg cgatctcctc gaccgaggtg ccatcgaacc
     1621 ctcgttcggc gaagagggag cgtccgacca cgagaagctg ctctcggcgc tcgtgggcgg
     1681 tcatgcgcga ccttggggga cgcttgggtt cggcgggcac gctctcagtg tgccctgaat
     1741 cctggcagac tgctctcctg gaccctcgcg gcgacggtcc gccctggtgt aatggcagca
     1801 tgcaggcctt tggagccttg cggttcaggt tcgaatcctg agggcggagc ccacggtcgg
     1861 cccctcgtgg gcccggtaga gtagtggcac acgacgttgg cgccgaagtc gggcatgagc
     1921 aagggggccc cttgagcgac gagcgacctg ccgcggtgat tgtcctcgcc gcaggagccg
     1981 ggacgcgcat gaagtccgcc atacccaagg tgcttcaccg catcggtggc cgcaccctcc
     2041 tccaccatgt gctcgccgcg gcggcggaca cccagccagg tcgcatcgtc gttgtcgtcc
     2101 gccacgaggc cgaactggtc tccgcgcacg tgcgggaggt cgcgcccgcc gcgctcatcg
     2161 ccgatcagga cgagattccg ggaactggcc gcgccgtgtg gggcgcgctc gtggcgctcg
     2221 acgccgccgc gaaggtcacg gggaccctgc tcgttacctc cggcgacgtt cccctcatgg
     2281 acggcgggac gctctcggca ctcgccgcct cgcaccgagc ggacggaagc gccgtcacgg
     2341 ttctcacgac gatcgtgacg gacccgacgg ggtacggacg catcatccgc gacccggaga
     2401 cgggcgacgt ggcccgcatc gtggaggagg gcgacgcgac cgagggcgag cgccagatca
     2461 cggagatcaa taccggtacc tacgcgttcg acgccggagc gttgcgatcc gcccttgccg
     2521 gaattggccg agcgaacacc cagggcgaga tgtacctcac ggacgtgatc gccgccgcga
     2581 gggcgtcggg tggcgtggtg cgcgcgatca tgatcgagga ctccgcggcg gcggagggca
     2641 tcaacgatcg ggtgcaactc tcgcgcgctg gcgcggaact caacgcccgg atcctggagc
     2701 gctggatgct ggacggcgtg acgatcgtcg atccggctac cacgtggatt gacgcgggcg
     2761 tcacgctcgc cgcggacgtg acccttctgc cgggaaccca tctgcacggg accaccgccg
     2821 tcgcgaagct cgcaacgatc gggccggaca ccacccttac cgatgtcatc gtgggcgagg
     2881 gcgccacgat cgtgcgctcc cacgggacgg gctccgtcat cggcgcgggc gcgagcgtcg
     2941 gccccttctc ctaccttcgt ccgggaacca acctcggcag ggacgggaag atcggcgcgt
     3001 tcgtcgagac caagaacgcg cagatcggcc gcggctccaa ggttccccat ctcagctacg
     3061 tcggggatgc gacggtggga gaggaatcca acatcggcgc ggcgacgatc ttcgtcaact
     3121 acgacggcgt gaccaagcat cacacgacgg tgggcagcca cgtgcggatt ggctcggaca
     3181 acacgctcat cgcgcccgtg acgatcggcg acggggcata cacgggcgcc ggcgcgatca
     3241 tccgccacga cgtccccgcg ggcgcgctcg cgatgaactc cgcgccccag cagaccaccg
     3301 aggggtgggt ggagcggcgg aggccgggca cgccctcggc aagggcggcg gaggccgcgc
     3361 gggatgccgc atccaagccc gaggcgctca cgccggaggc acacaacgaa agggccgccg
     3421 cgaggcggtc cggggccaag ggagagtcat gagtgccggg atctcgaacg cgaacgagcg
     3481 acggctcgtg ctggtctctg ggagggcgca cccggaactc gcgcttcagg tggcggaggg
     3541 tctcggcacg gagctcttgc ccaccgacgc ctatagcttc gcgaatggcg aactgtacat
     3601 ccgtttcggg gagagcgttc gcggcgcgga cgccttcgtc cttcagtcgc acgcggcgcc
     3661 catcaacaag gcgatcatgg agcaactgat catggtcgac gcgctcaagc gcgcctccgc
     3721 cgcgcgaatc acggtcgtgg cgccctgcta cggatacgcc cgtcaggaca agaagcaccg
     3781 tggccgcgag cccatctccg cccgcctcat ggccgacctc ttcaagacgg ctggcgc
//
LOCUS       DASVKP010000102         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1438255, whole genome shotgun sequence.
ACCESSION   DASVKP010000102 DASVKP010000000
VERSION     DASVKP010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438255"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1913
                     /locus_tag="VF362_02195"
     CDS             <1..>1913
                     /locus_tag="VF362_02195"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX7589768.1"
                     /translation="YSRALPDARDGLKPVQRRIVYMMEQMGLRPERSYVKSARVVGEV
                     MGKLHPHGDQAIYDALVRMAQSFSLRVPLVDGHGNFGSLDDGPAAARYTEARLAPAAM
                     ALTADIGEDTVEFVPNYDNKLTQPSVLPAAIPNLLVNGASGIAVGMATNMAPHNLVEV
                     ISAARHLLAHPDATTADLMKFVPGPDLPGGGKIVGLDGIVQAYETGKGAFRTRATTRV
                     EKVSARREGIVVTELPYLVGPEKVIEKIKEAVAGRRLEGIAAVTDLTDRHHGLRLVIE
                     IKSGFNPDAVLEQLFRLTPLEDTFSINNVALVDGEPLQLGLRELLRVWVEHRIEVVRR
                     RSSYRLQARLDRVHLVNGLLVAILDIDEVIQIIRAADDAAVARTRLMTAFDLSEAQAE
                     YILELRLRRLTKFSRVELETEKAKLTAEILDLEEILGSDASLRAVVGREMDAAAAAHG
                     TPRRTILLSDGGSLAVASGAKAGAKGAKGAAGASLEIADSACSALLSVTGLVARTADD
                     HPVSRDGRRSAHDALRSIIPASARSDVGVVTSLGRIVRLSLLDVPALPPTDEPPSLGG
                     GVPIGEVISLERGEEALALVPLGGSVPVALGTAQGVVKRLAPEEAPQRDAWGVIALRP
                     GDSVIGAAVAPDD"
BASE COUNT          273 a          717 c          657 g          266 t
ORIGIN      
        1 ctactcgcgc gcgctgcccg acgcgcgcga cggcctcaag cccgtccagc ggcgcatcgt
       61 ctacatgatg gaacagatgg ggctccgccc cgagcgctcg tacgtgaaga gcgcgcgtgt
      121 cgtcggcgag gtcatgggga aactccaccc ccacggcgac caggccatct acgacgccct
      181 ggtgcgcatg gcgcagtcgt tctcgctgcg cgtgcccctg gtggacgggc acggcaactt
      241 cgggtccctc gacgacggcc ctgccgcggc ccgctacacc gaggctcggc tcgcgcccgc
      301 ggccatggcg ctcaccgcgg acatcggcga ggacaccgtc gagttcgtcc ccaactacga
      361 caacaagctc acgcaaccat ccgtccttcc cgccgcgatc cccaacctcc ttgtcaacgg
      421 ggcctccggc atcgccgtcg gcatggcgac caacatggcg ccccacaacc tcgtcgaggt
      481 catctcggcc gcgcgccacc tcctcgccca cccggatgcc accaccgctg acctgatgaa
      541 gttcgtcccc ggccccgacc tgcccggcgg cggcaagatc gtcgggctcg acggcatcgt
      601 ccaggcgtat gagacgggca agggcgcgtt ccgcaccagg gcgacgacgc gggtggagaa
      661 ggtctccgcg cggcgcgagg gcatcgtcgt caccgagctt ccgtacctcg tggggccgga
      721 gaaggtgatc gagaagatca aggaggcggt cgcggggcgg cggctcgagg ggatcgccgc
      781 cgtcacggac ctgaccgacc ggcaccacgg gctgaggctc gtcatcgaga tcaagagcgg
      841 gttcaacccg gacgccgtcc tggagcaact cttccgcctc accccgctgg aggacacgtt
      901 ctccatcaac aacgtcgcgc tcgtcgacgg cgagccgctc cagttggggc tgcgcgaact
      961 cttgcgcgtg tgggtggagc accggatcga ggtggtgcgg cggcgcagct cctaccgcct
     1021 ccaggcgcgg cttgaccgcg tgcacctggt gaacgggctg ctggtcgcga tcctggacat
     1081 cgacgaggtc atccagatca tccgggcggc ggacgacgcc gcggtggcgc ggacccggct
     1141 catgacggcc ttcgacctca gcgaggccca ggcggagtac atcctcgagt tgcgcctgcg
     1201 gaggctgacc aagttctccc gggtggaact cgagacggag aaggccaaac tgacggcgga
     1261 gatcctcgac ctggaggaga tcctcgggag cgatgcgtcg ttgcgcgccg tcgtggggcg
     1321 cgagatggac gccgccgccg cggcccacgg gacgccgcgc cgaaccatcc tgctgtcgga
     1381 cggcggatct ctcgcggttg cttccggcgc caaggccggc gcgaagggcg cgaagggcgc
     1441 ggcgggagcg tcgctcgaga tcgcggactc cgcgtgctcc gcgctcctgt ccgtcactgg
     1501 gctcgtcgca cggaccgccg acgaccaccc cgtctcccgc gacgggcggc gctccgccca
     1561 cgacgcgctc cggtccatca tcccggcttc cgcccgctcc gacgtcggcg tcgtgacgag
     1621 ccttgggcgc atcgtgcgcc tgtccctcct cgacgtcccg gccctgcccc ccacggacga
     1681 gccgccctcg ctcggcggcg gcgtcccgat cggcgaggtc atctccctcg agcgcggcga
     1741 ggaggccctc gcgctcgtcc cgctcggcgg atccgttccc gtcgccctcg ggaccgcgca
     1801 gggcgtcgtc aagcgcctcg cgccggagga ggcgccgcag cgcgacgcct ggggggtcat
     1861 cgcgctgagg cccggcgact ccgtgatcgg cgcggccgtc gcccccgacg acg
//
LOCUS       DASVKP010000103         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_284468, whole genome shotgun sequence.
ACCESSION   DASVKP010000103 DASVKP010000000
VERSION     DASVKP010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284468"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VF362_02200"
     CDS             <1..263
                     /locus_tag="VF362_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HEX7589769.1"
                     /translation="WMLKVADKKKALFYVVPLLEMFRVSMTVRETERDELLGRRALAP
                     LHPAMREARKSSEGYALYFAVDGSDECAQVELLVSELIGLRR"
     gene            319..1560
                     /locus_tag="VF362_02205"
     CDS             319..1560
                     /locus_tag="VF362_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7589770.1"
                     /translation="MTSLSPRHTRLAILALALGGFSIGATEFIAMGLLPEIARDLLPD
                     LYATDSAAAIAKAGWLITAYAVGVVVGAPTIAAAAARFPRRTLLIALTIGFTLGSVAS
                     AVAPTFGLVVAARFAAGLGHGAFFGVAALVAARLMGPEKRGQAVASVLVGLTIASVVG
                     VPSITFLGQHAGWRWAYVVVAVLFALTTLAIAAFIPRTEGDPHATIRRELRAFRRPAV
                     WLALLTGSIGFGGFFAVYSYVAPLSTEVAGTSTGFVPIVLMVLGLGTTVGILGGGRLA
                     DRGAVRAIFLLFGALAVSLTVLGLSAHSVPGLLVGVFLVGGSAQALAPALQTRLMDVA
                     GESQTLAAAVNHSALNIGNSLGAALGGAVIAAGLGYLAPTWLGLGLCVPGVLFALGGW
                     AVTRHKQQVTSPVAAATEGAR"
BASE COUNT          201 a          613 c          605 g          290 t
ORIGIN      
        1 ggtggatgct caaggtcgcc gacaagaaga aggcgctctt ctacgtggtc ccgctcctgg
       61 aaatgttcag ggtcagcatg actgttcgcg agaccgagag ggacgaacta ctgggtcggc
      121 gggccctggc gccgcttcac cccgcgatgc gcgaggcgcg caagtcctcg gagggctacg
      181 cgttgtactt cgcggtggac gggtccgacg agtgcgcaca ggtggagttg ctggtgtcgg
      241 aactcatcgg gttacggcgg tgacgcgcga cggccgtgcg gtggggcgcg cagggtcggc
      301 acctaggatc gaagcatcat gaccagcctc agcccccgcc atacgcgcct cgcgatcctg
      361 gctctcgccc taggcggctt ctccatcggc gccacggagt tcatcgcgat ggggctcctg
      421 ccggagatcg cccgcgacct cctcccggac ctctacgcca cggactccgc cgcggcgatc
      481 gcgaaggcag gctggctcat caccgcgtac gcggtcggcg tcgtcgtggg cgccccgacc
      541 atcgcagcgg cggcggcgcg cttcccgcgt cggaccctcc tgatcgccct caccatcggc
      601 ttcacgctcg gctccgtcgc ctccgccgtc gccccgacct tcgggctcgt cgtcgccgcc
      661 cggttcgccg cggggctcgg ccacggcgcg ttcttcggcg tcgccgcgct cgtcgccgcg
      721 cgcctcatgg ggccggaaaa gcgcggccag gccgtcgcct cggtgctcgt cggcctcacc
      781 atcgcgagcg tcgtcggcgt cccgagtatc acgttcctcg gccagcacgc cggctggcgc
      841 tgggcgtacg tggtcgtggc ggtgctcttc gccctgacga cgctcgccat cgcggcgttc
      901 attccgcgca ccgagggcga cccccacgcg accatccgcc gcgaacttcg cgccttccgg
      961 cgccccgccg tctggctcgc gctcctgacc ggctcgatcg gatttggcgg cttcttcgcc
     1021 gtctacagct acgtcgcgcc gctctccacg gaggtcgcgg gcacgtcgac cggattcgtg
     1081 ccgatcgtgc tcatggtcct tggcctgggg acgacggtcg ggatcctggg cggagggcgc
     1141 ctcgccgacc gcggggccgt ccgggcgatc ttcctcctct ttggggcgct ggcggtctcg
     1201 ctgacggtcc tcgggctttc cgcgcactcc gttccggggt tgctcgtcgg ggtcttcctc
     1261 gtgggcgggt ccgcgcaggc gctcgcgcca gcgctccaga cccggctcat ggacgtcgcg
     1321 ggggagagcc agacgctcgc cgcggcggtg aaccactcgg cactcaacat cggcaactcg
     1381 ctgggcgcgg cgctcggcgg ggccgtcatc gcggccggac tcgggtacct ggcgccgacg
     1441 tggctcggac tagggctatg cgttccgggc gtgctgttcg cgctcggcgg gtgggccgtc
     1501 acgcggcaca agcaacaggt gacctcgccg gtcgccgcgg cgacggaggg tgcgagatga
     1561 gggagatcgc ggcaaggttc tgccacgccg agtactccga cgagcggctc gccgagagca
     1621 tcggcgacgt gctcacgaac agcgagttgg cggcgttggc gtccatcgat ggcgcggagt
     1681 cgcatgtcgc gacggtctcc ttcgcattc
//
LOCUS       DASVKP010000104         1853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049 k141_37176,
            whole genome shotgun sequence.
ACCESSION   DASVKP010000104 DASVKP010000000
VERSION     DASVKP010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37176"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VF362_02210"
     CDS             <1..886
                     /locus_tag="VF362_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7589771.1"
                     /translation="ADRDTGVLLIRDAGSATDTAWMHHRADLPQLIRAGRFIARPKRY
                     LRGFARELERVEDLPLAASEEALRGDGWVKIIADWIDRDLGSDGDLRPLWPDDVLADA
                     VAAAHAAGARVTAHTFATEAVGGLLDAGIDCIEHGTGMKEAHIAEAARRGIAVVPTLL
                     QVGQFASIADQGSAKFPAFAARMQAMYQARYAQVRRFHEAGVPLLVGTDAGGTIGHGS
                     FAQECAEMVAAGLPARDVVAAATWVARRFLGAPGIEEGASADVVVFRGDPREDIGILA
                     EPSAVVLRGAVFDPRRGA"
     gene            896..1750
                     /locus_tag="VF362_02215"
     CDS             896..1750
                     /locus_tag="VF362_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX7589772.1"
                     /translation="MTNPGSAVWGTTIHSMAMPFAIEIVRGDPSLVGDPGPSEGECAA
                     AVAAFHRDLSWADDVFSLWRDDTPLARLARGEITVDDCPPAVGEVIAECERYREITAG
                     AFDARRPDGVLDPSGIVKTWAVDRAAWRLESLGAAGWLVGASGDVLVSGLAPGMVPWT
                     VGIADPRGADDPQGGPTLDVVALGGGMDAPFRALATSGTAQHGEHIWNPATGLRESAF
                     VQASVIGTDLVECDAWATAIVAGGADVAIAAQRRGLEVLCVPRPGVGADPAEAVPTLA
                     SPGWPGVA"
BASE COUNT          233 a          656 c          706 g          258 t
ORIGIN      
        1 ggccgatcgc gacaccgggg tgctcctcat ccgcgacgcc gggagcgcga ccgacaccgc
       61 gtggatgcac caccgcgccg acctgcccca actgatccgc gccgggcgct tcatcgcccg
      121 cccgaagcgc tacctccggg gcttcgcgcg cgaactggag cgcgtcgagg acctcccgct
      181 cgcggcgtcc gaggaggccc tgcgcggcga cggctgggtg aagatcatcg ccgactggat
      241 cgaccgcgac ctgggctccg acggcgacct ccgcccgctc tggcccgacg acgtccttgc
      301 ggacgccgtc gcggcggcgc acgccgccgg cgcccgcgtg accgcgcaca ccttcgcgac
      361 ggaggccgtg gggggtctcc tcgacgcggg gatcgactgc atcgaacacg ggaccggcat
      421 gaaagaggcc cacatcgccg aggcggcgcg gcgcggcatt gccgtggtcc cgacgcttct
      481 gcaggtgggg cagttcgcct cgatcgccga ccagggcagc gcgaagttcc cggcgttcgc
      541 cgcgcggatg caggccatgt accaggcacg atacgcgcag gtgcgtcgat tccacgaggc
      601 gggcgttccc cttctggtcg ggacggacgc cggcggaacc atcggccacg gctcgtttgc
      661 ccaggagtgc gccgagatgg ttgcggcggg gttgcccgcc cgcgacgtcg tcgcggccgc
      721 gacgtgggtg gcgcggcggt tcctcggcgc cccggggatc gaggagggcg cgagcgccga
      781 cgtcgtcgtg ttccggggcg atcctcgcga ggacatcggg atcctcgccg agccctcggc
      841 ggttgtgttg cggggcgccg tgttcgatcc gcggcgcgga gcctaggctg atgccgtgac
      901 gaacccgggg tccgccgtgt ggggaacgac catccactcc atggcgatgc cgttcgcgat
      961 agagatcgtt cgcggcgatc cctccctggt gggtgacccc ggcccgagcg aaggggagtg
     1021 cgcggccgcc gtcgccgcat tccaccgaga cctcagctgg gcggacgacg tcttctccct
     1081 ttggcgggac gacacgcccc tcgcgcggct cgcgcgcggg gagatcaccg tcgacgactg
     1141 cccgcccgcc gtcggcgagg tgatcgcgga gtgcgagcgg tacagggaga tcaccgccgg
     1201 cgcgttcgat gcgcggcggc ccgacggtgt cctcgatcct tcgggaatcg tcaagacctg
     1261 ggccgtcgac cgtgccgcgt ggcgcctcga gtcgctgggc gccgcggggt ggctcgtcgg
     1321 cgcctcgggc gacgtgctcg tctccggcct cgcgcccggg atggtcccgt ggacggtggg
     1381 aatcgcggat ccccgcggcg cggatgaccc gcagggtggg ccgaccctcg acgtcgtcgc
     1441 tctcggaggc ggcatggacg cgccgttccg cgcgctggcg acatccggca cggcgcagca
     1501 cggcgagcac atctggaatc cggcgacggg gctgagggag tcggcgttcg tgcaggcgag
     1561 cgtgatcggg acggacctcg tggagtgcga cgcgtgggct acggcgatcg tcgcgggcgg
     1621 cgccgacgtc gcgatcgcgg cgcagcggcg cgggctggag gttctctgcg ttccgcgccc
     1681 cggggtgggc gccgaccccg cggaggccgt tcccaccctc gcgagccccg ggtggccagg
     1741 cgtcgcctga gaaggctctc agaattgccc cggaacactg ggaccatgac gacgacttct
     1801 ttgggggccc gcgagatgga acgccgcctc ggcttcgccg agcacgccga acg
//
LOCUS       DASVKP010000105         3732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1108585, whole genome shotgun sequence.
ACCESSION   DASVKP010000105 DASVKP010000000
VERSION     DASVKP010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108585"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VF362_02220"
     CDS             <1..648
                     /locus_tag="VF362_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7589773.1"
                     /translation="DEPLPHPASEAAAPSLAGRTLLASGLGVETPDGSALAPASLTFV
                     ASPGEVVALVGANGEGKSTALMAMAGLIPPSRGAVGLAAAGGTPAMTLATAEGGVDAE
                     GWSAQVAWVPQRPDLGPSALTLSLGQRQRLALERAFASGRPVLLLDEPTAHLDPPARA
                     EVARRARAAADGGAVVIIATHDDAIRATADHVVAVSSRGTVSSGAPSRPATEVAP"
     gene            645..2351
                     /gene="cydC"
                     /locus_tag="VF362_02225"
     CDS             645..2351
                     /gene="cydC"
                     /locus_tag="VF362_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEX7589774.1"
                     /translation="MMRGARALLGELEISWRTIALAIAAGSAALGSAVLLGATSAWLI
                     ARAAQMPSPADLAIAAVIVRTLGITRGAFRYAERLVSHDAALRGMTNLRARAYERLER
                     RGTARVLSLPRGDIVARTGPDVDAMGDAVSRSIVPAGIAVVVSVLSVVVVGSQDARAG
                     LALAAALAAAAIVPAALTVRSLRVTQERGARADAAATIAALSLVEAGPEHRVWGTTSQ
                     AHAALVAADREVERARDAAARPAALAAALQVAFAGLGLVAAVAIGIAAARAGGLDGPA
                     AAVVALTPLGAFEAVSALPAAAAQWFRTRAAAERVFHLVDPSSPVASEPRPGKPRPGD
                     VGAAPSPRAASRNAANHGAAPTLRLTGLRVAWPGATPTPPVSAVIPPGGVLGVIGASG
                     VGKTTLLLTLAGALAPAEGTVTIDGRPVDGGDTGGIVAMTAEDAHVFGTTILENLRVA
                     RGDVTAGEAREALSAVGLGPWLAAQAAGLDTELGSGGLTVSGGERRRLLLARVALHPA
                     PIHLIDEGAEHLDPEGADALRALVRRRSAARHSTILVTHDPSLLDVVDTVIDLGPRTE
                     EP"
     gene            2348..>3732
                     /locus_tag="VF362_02230"
     CDS             2348..>3732
                     /locus_tag="VF362_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7589775.1"
                     /translation="MTDSLSSAIIALNAELELPAVLDRFLEVSLAHTGAKYAAINVLN
                     DHGESVDFYYQGMDRSIWERIGRGPGSVGVLGRIPSLGTLVVDEVTAHPAFEGLPAGH
                     PPLGSFLGTALRVRDHVFGYLYLASKEGGFTSDDEPIVQALAAAASVAIDNSQLYASA
                     LERERWLTASQEITTALLADPGDETALARIVETAQELARASAATLVLPGVDDKWVMEF
                     TAGHRAGDLLGLALPEGGQALKTIRAGEGTIAQEPPGEVILEPVRDFGPALYAPLRTE
                     GRTVGLLMLWRDKGQTTFDQSDLATAQRFADQAALALSVAELRHVKDVTALLEDRQRI
                     ADDLHDLVSQELFAASIQLESLGERVPAEFRAGLSGCLEHVKRAQREVRGVVSTLDNE
                     RAAEPLSGRLRREIILAQDSLGFAPVVKVDWSAIPPEVEADAALGDDLVAVLRESLSN
                     VAQHARASQAVV"
BASE COUNT          461 a         1435 c         1323 g          513 t
ORIGIN      
        1 gacgagccgc tcccccaccc ggcatccgaa gccgccgcgc cgtcgctcgc ggggcgaacg
       61 ctcctcgcga gcggcctcgg cgtcgagacg ccggacggct ccgccctcgc gccggcctcg
      121 ctcacgttcg ttgcgtcccc cggcgaggtg gtcgcgctcg tcggcgcgaa cggcgagggg
      181 aagtccaccg cgctcatggc catggcgggc ctcatcccgc cgagtcgcgg ggccgtcggg
      241 ctggcggctg cgggaggcac gccagcgatg accctcgcca ccgccgaggg cggcgtggat
      301 gccgaggggt ggtcggcgca ggtggcgtgg gttccccagc ggcccgatct cggcccgtcg
      361 gccctcaccc tgagcctcgg ccagcgccag cgcctcgccc ttgagcgcgc gttcgcctcc
      421 ggccgccccg tgctgcttct cgatgagccc accgcccacc ttgatccgcc cgctcgggcc
      481 gaggtcgcgc gccgcgcaag ggccgcggcg gacggcggcg ccgtcgtcat catcgccacc
      541 catgacgacg cgatccgggc gaccgccgat cacgtcgtcg cggtttcgtc gaggggcacc
      601 gtctcgtcgg gtgccccctc ccggcccgcg acggaggtgg cgccatgatg agaggggcgc
      661 gcgccctcct cggggagctc gagatctcgt ggcgcaccat cgccctggcg atcgccgcgg
      721 gaagcgccgc gctgggctcc gcggtcctgc tcggggcgac ctcggcgtgg ctgatcgccc
      781 gcgcggcgca aatgccgtcc cccgccgacc tggccatcgc cgccgtgatc gtccggaccc
      841 tcggcatcac caggggcgcg ttccggtacg ccgagcgcct cgtctctcac gacgccgccc
      901 tgcggggaat gacgaacctc agggcgcgcg cgtacgagcg cctcgagcgg cgcggcacgg
      961 cgcgcgtcct gtcgctccca cgcggcgaca tcgtcgcgag gacggggccc gacgtcgacg
     1021 ccatgggcga cgcggtctcg cggtcgatcg ttcccgcggg aatcgcggtg gtggtgtcag
     1081 tcctctccgt tgtcgtcgtc ggctcgcagg acgcccgcgc gggcctcgcc ctcgcggcgg
     1141 ccctcgcggc cgcggcgatc gttcccgcgg cgctgaccgt gcgctccctc cgcgtgaccc
     1201 aggagcgggg ggctcgcgcc gacgcggccg cgacgatcgc ggccctctcg ctcgtcgagg
     1261 cgggtccgga gcatcgggta tggggcacga cgagccaggc gcacgccgcg ctcgtggcgg
     1321 ccgacaggga ggtggagcgg gcgagggacg cggccgctcg gcccgcggcg ctcgccgcgg
     1381 cgctccaggt ggcattcgcc ggcctcggct tggtcgccgc ggtggccatc ggaatcgcgg
     1441 cggcccgtgc gggcgggctc gacggccccg ccgccgccgt cgtcgcgctc accccgctcg
     1501 gggcgttcga ggccgtgtcg gccctccccg ccgccgccgc gcagtggttc aggacccgcg
     1561 cggccgccga acgggtcttc cacctcgtgg atccctcctc cccggtcgcg tccgagccgc
     1621 gtcccggcaa gcctcgcccc ggcgacgtcg gcgcggcgcc gtcgccccgc gcggccagcc
     1681 gaaatgccgc gaaccatggc gcggcgccca ccctgcgcct caccgggctc agggtggcct
     1741 ggccgggcgc gacgccgacg ccacccgttt cggccgtcat tcccccgggc ggggtcctgg
     1801 gcgtcatcgg tgcgtccggc gtcgggaaga cgaccctgct tctgacgctc gcgggcgcgc
     1861 ttgccccggc cgagggcacg gtcaccatcg acggacgacc cgtggacggc ggcgacacgg
     1921 gaggcatcgt cgcgatgacc gcggaggatg cccacgtgtt cggcacaacg atcctggaga
     1981 acctcagggt ggcgcgcggc gacgtgacgg cgggcgaggc ccgcgaggcc ctttcggccg
     2041 ttggcttggg gccgtggctc gccgcgcagg ccgccgggct cgacaccgaa ctcggtagcg
     2101 gggggctcac ggtgtcggga ggcgagcgac gccggctgct cctcgccaga gtcgcgctcc
     2161 atcccgcgcc cattcacctg atcgacgagg gtgccgagca cctcgacccc gagggtgccg
     2221 acgcactccg ggccctcgtc aggcgacgct cggcggcgcg ccacagcacc atactggtga
     2281 cgcacgaccc gagccttctc gacgtggtag acacggtcat agacctcgga cccagaacgg
     2341 aggagccatg accgactcgc tcagcagcgc gatcatcgcg ctcaacgcgg aactggaact
     2401 gcccgcggtg ctcgaccggt tcctcgaggt ctcgctcgcc cacacgggcg ccaaatacgc
     2461 ggcgatcaac gtcctcaacg atcacgggga gtccgtggac ttctactacc aggggatgga
     2521 tcgctccatc tgggagcgga tcggccgcgg ccccgggtcc gtcggcgtcc tcggtcgcat
     2581 cccctcgctc gggacgctcg tggtggacga ggtcaccgcc catccggcct tcgagggcct
     2641 ccccgccggg catccgccgc tcggctcgtt cctcgggacc gcgcttcgcg tgcgcgacca
     2701 cgtcttcggc tacctctacc tcgcgagcaa ggagggcgga ttcacctccg acgacgagcc
     2761 catcgtccag gccctcgcgg cggccgcctc ggtcgcgatc gacaactcgc aactgtatgc
     2821 aagcgcgctt gagcgcgaac gatggcttac ggcctctcag gagatcacaa cggccctgct
     2881 cgccgatccc ggcgacgaga ccgccctggc gcgaatcgtc gagacggcgc aggaacttgc
     2941 gcgcgcttcg gccgcgacgc tcgtgcttcc cggagtcgat gacaaatggg tcatggagtt
     3001 caccgcgggc caccgcgcgg gcgacctcct cggcctcgcc cttcccgagg gtgggcaggc
     3061 gctcaagacc atccgggccg gcgagggcac gatcgcccag gagccgccgg gcgaggtcat
     3121 ccttgagccc gtccgcgact tcggacccgc cctctacgcg ccgcttcgca ccgagggtcg
     3181 gaccgtcggg ctgctcatgc tctggcgcga caagggccag accaccttcg accagtccga
     3241 cctcgccacc gcgcagcgat tcgcggacca ggcggccctc gcgctgtccg tcgccgaact
     3301 gcgccatgtc aaggatgtga ccgccctcct ggaggatcgg caacgcatcg ccgatgacct
     3361 ccacgacctg gtctcacagg aactgttcgc ggcctcgatc caactggaga gcctcggcga
     3421 gcgggttccc gccgaattcc gcgcgggact gagcggttgc ctcgagcacg tgaagcgggc
     3481 ccagcgcgag gtgcggggag tggtgtcgac cctcgacaac gagcgggcgg cggagccgct
     3541 ctcgggcagg ctccggcgcg agatcatcct cgcccaggac tcgctcggct tcgcgcccgt
     3601 cgtcaaggtg gattggtccg cgatcccccc ggaggtcgag gccgacgccg cgctcggcga
     3661 cgacctcgtt gccgtgctcc gcgagagcct ctccaatgtt gcccagcacg cccgcgcctc
     3721 gcaggcggtg gt
//
LOCUS       DASVKP010000106         6105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_203478, whole genome shotgun sequence.
ACCESSION   DASVKP010000106 DASVKP010000000
VERSION     DASVKP010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203478"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="VF362_02235"
     CDS             <1..1258
                     /locus_tag="VF362_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEX7589776.1"
                     /translation="RSDSLDPTVHRTTPPPRFTQGSMIKELEERKFGRPSTYAATVDL
                     IVARQYVRIDRQALIPTWIAFSIVGLMEKHFDWLIDYDFTADMELGLDRIAAGELDRR
                     DWLSNFYLGTGSSEHGRQEGLKHLVDGLGAIDARAINTFELGDGIAVRVGKFGPYVER
                     TVDGEAQRASVPEDLAPDELTVEKAVELLSNQGGEDRVLGEHPETGHTIVAKSGRYGP
                     YVTEVLPEGDKAKPKTGSLFASMSVDGVTLEQAVTLLALPRVVGVDPATDEEITAQNG
                     RYGPYLRKGTDSRTLESEDQILTMTLEGALAIYAEPKRGRGRPVTPPLKELGTDPTTE
                     KPIVIKEGRFGAYITDGETNITVPRGEHIEELTHERAVELLAEKRAKGPTAKKRRAPA
                     KKAPAKKATVKKVAVKKPAAKKPPAR"
     gene            1341..2360
                     /locus_tag="VF362_02240"
     CDS             1341..2360
                     /locus_tag="VF362_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7589777.1"
                     /translation="MTNPSPVVALDPKSAPSIRWGIVGVGWMADQFANGVNAHTHSTV
                     HAVSSRSLEKARAFAASHSVAKAYGSVAELVADPDIDVIHIASPHSEHLANALEAIAA
                     GKNVLVEKPICRNSSEARVLIDAARAANVFVMEAMWTRYLPHMIELKRVIAAGEIGEI
                     VSLSADHGQAFPFVPGSRWWEPSLAGGALLDLGIYPVAFAHDILGVPDSIIAAGQMTE
                     TKVDGQVSMILGYGDKTQASLQTTLWSQTPTVAAISGTKGRIEISENFYREASFRVIR
                     PDGVREFPYTGVANGYAYEAAEVARRITAGEKQSPAHTPEMTLEVMATLDEVRRQIGL
                     TYPGE"
     gene            2386..2847
                     /locus_tag="VF362_02245"
     CDS             2386..2847
                     /locus_tag="VF362_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEX7589778.1"
                     /translation="MALARAPLPRAHLDGAHVRLAAVIAAGGAAGALLRWGIGREWPV
                     LPGQFPWSTLWINVTGCLAIGVLMALIEEVLAGRVYVRPLLGVGLLGGFTTFSTFAVE
                     TRALLGAHPALALVYYLGTPAAAVLATIVGAVLVTSALKARTWFASRRSIA"
     gene            2844..3194
                     /locus_tag="VF362_02250"
     CDS             2844..3194
                     /locus_tag="VF362_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX7589779.1"
                     /translation="MTTIASPALRLTVIVDEAVQWHHHPLYTEIVHRAHAAGLAGATV
                     IRGVEGYGASGHIHTTRILALSEALPVLILIIDEEKKIRAFLPELDELVVRGIVVLDG
                     VEVVRYVGEAREAG"
     gene            3191..3553
                     /locus_tag="VF362_02255"
     CDS             3191..3553
                     /locus_tag="VF362_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEX7589780.1"
                     /translation="MIPVAVALGAAVGAPARYFTDRYVQSRTASVFPWGTLTVNAAGS
                     LVLGLLVGSGASGTLAALAGVGFCGALTTYSTLGYETHRLIRDRSMLLAALNVLGSLV
                     VGLAAASLGYWVATAVAA"
     gene            3550..4821
                     /locus_tag="VF362_02260"
     CDS             3550..4821
                     /locus_tag="VF362_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7589781.1"
                     /translation="MNGMRSDDAQSGGRKASVPRPGRAPYSPWRFVVAMGFVSMLADV
                     VYEGSRAITGPFLESLGVSAVAVGAITGVGEAIGFAGRLGSGPLADKTRAYWPLLIVG
                     YALTVVAVPFLGVTSVVWIVATLIFIERAGKAVRRPAKDVILSHATSGIGRGRGFAVH
                     EALDQFGAVLGPLAVAGAFHLTHNYAPTFAMLALPGAIVMVLLLRLRKRVPDPSAYEE
                     ALAAELAISPPSAGRRAAAHPPLGRPYWWYLSFATVTTIGYSTFGVLGYHLVHEGLAS
                     TATVPVIYAGAMIVDALAALAVGALFDRVGRRVLLVVPMLAAAIPPLAFARSLPLAIA
                     GMLVWGAVMGIQESVLRAAVADLIPSARRGTAYGIFAAAIGAAALVGGILTGGLYETH
                     LPLLVAVIAGIQVAALVLYVAWSRGLRSEVP"
     gene            4826..5515
                     /gene="tmk"
                     /locus_tag="VF362_02265"
     CDS             4826..5515
                     /gene="tmk"
                     /locus_tag="VF362_02265"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX7589782.1"
                     /translation="MAYPGFFLVFEGGDGVGKTTQATMIARWLASPEGGTRYVTITRE
                     PGGTPLGLQLREAVMHGGDVSPRAEALLYAADRAQNVDEVVFPALEQGDIVVQDRFID
                     SSIAYQAVARGLGEEIGRISAWAADGLIPDLTIVLDMEPDASRLGEDLDRVEQDTWAR
                     AGEIRQAFLDLAEREPGRYLVLDASRPLEVVHSDICSALQAVLPEPSRGFRAPKPVPR
                     VGNWTQWDLSE"
     gene            5512..>6105
                     /locus_tag="VF362_02270"
     CDS             5512..>6105
                     /locus_tag="VF362_02270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX7589783.1"
                     /translation="MSVWETVVGQGDALDPLIAAVEGDRAMTHAWLITGPPGSGRSIA
                     ARAFAAALQCPSGGCGECHACRTVLAGAHPDVRIVATEKVTITVDEVRSLVSNAQSTP
                     SQGRWRVIIIEDADRMTERTSNVLLKAVEEPPPRTAWILCAPSLHDVATTIRSRCRVV
                     TLRIPSVEVVTALLVERDGIQPELARECALAAQCHIGV"
BASE COUNT          879 a         2197 c         2070 g          959 t
ORIGIN      
        1 gcgctcggac tcgctcgatc cgaccgtgca ccgcacgacg ccgccgccgc gattcaccca
       61 gggctcgatg atcaaggagc tcgaggagcg caagttcggc cgcccctcca cctacgccgc
      121 gacggtggac ctgatcgtcg cgcgccagta cgtgcgcatc gaccgccagg cgctcatccc
      181 cacgtggatc gcgttctcca tcgtcggcct catggagaag cacttcgact ggctcatcga
      241 ctacgacttc accgccgaca tggagctcgg gctcgaccgc atcgcggcgg gcgagttgga
      301 ccgccgcgac tggctttcga acttctacct cggcaccggc tccagcgagc acggccgcca
      361 ggagggcctc aagcacctgg tcgacggcct cggggcgatc gacgcccgcg ccatcaacac
      421 gttcgagctg ggcgacggca tcgcggtgcg cgtgggcaag ttcggcccct acgtggagcg
      481 gacggtggac ggcgaggccc agcgggcatc cgtcccagag gacctcgcgc ccgacgaact
      541 gaccgtggag aaggccgtcg agctactcag caatcagggc ggcgaggatc gcgtgctcgg
      601 cgagcacccg gagaccggac acaccatcgt cgcgaagtca ggccgctacg gcccctacgt
      661 gaccgaggtg cttccggagg gcgacaaggc gaagccgaag accggctcgc tcttcgcttc
      721 gatgtcggtg gacggcgtga cgctcgagca ggccgtgacg ctcctggctc tgccgcgcgt
      781 cgtcggggtg gaccccgcaa cggacgagga gatcaccgcc cagaacggca ggtacggtcc
      841 gtacctccgc aagggcaccg actcgcgcac gctcgagagc gaggaccaga tcctcacgat
      901 gacgctcgag ggcgcgctcg cgatttacgc cgagcccaag cgcggccgcg gccgcccggt
      961 gactccgccc ctcaaggaac tgggcaccga tcccaccacc gagaagccca tcgtcatcaa
     1021 ggagggccgc ttcggcgcgt acatcaccga cggcgaaacc aacatcacgg tgccgcgcgg
     1081 cgagcacatc gaggaattga cccacgagcg ggcggtggag ctgctcgccg agaagcgcgc
     1141 caagggcccg acggccaaga agcgccgcgc gccggcgaag aaggctcccg cgaagaaggc
     1201 cacggtcaag aaggttgcag tgaagaaacc agccgcgaag aagccaccag ctaggtagcc
     1261 cgcggtgaag aagactccgc cagacgcgtc ctagcccggc ggctgtgtcg cgacgaggct
     1321 cgacgggtta tcgtaacgtt atgaccaacc cctcgcccgt agtagccctc gaccccaagt
     1381 ccgcgccctc gatccgctgg ggcatcgtcg gcgtcggatg gatggcggac cagttcgcca
     1441 acggggtcaa cgcgcacacg cactccaccg tccacgccgt ctcgtcccgc tcccttgaga
     1501 aggcgcgggc cttcgccgcg agccactccg tcgccaaggc gtacggttcc gtcgccgaac
     1561 tcgtcgcgga ccccgacatc gacgtcatcc acatcgcgag ccctcacagc gagcaccttg
     1621 ccaacgcgct cgaggcgatc gccgcgggca agaacgtcct ggtggagaag cccatctgtc
     1681 ggaactcctc cgaggcccgc gtgctgatcg acgccgctcg cgcggccaac gtcttcgtca
     1741 tggaggccat gtggacgcgc tacctgcccc acatgatcga actcaagcgc gtcatcgccg
     1801 ccggcgagat cggcgagatc gtctcgcttt cagccgacca cggccaggcc ttcccgttcg
     1861 tccctggtag ccggtggtgg gagccctcgc tcgcgggagg cgcgctgctg gacctcggca
     1921 tctaccccgt cgcgttcgcc cacgacatcc tcggcgttcc agactccatc atcgcggcgg
     1981 gccagatgac cgaaacgaaa gtcgacggcc aggtcagcat gatcctcggc tacggcgaca
     2041 agacccaggc gtcgctccag acgacgctgt ggtcgcagac accgacggtc gcggccatct
     2101 cgggcacgaa ggggcggatt gagatctcgg agaatttcta ccgcgaggcg tccttccgcg
     2161 tgatccgccc cgacggtgta cgcgagttcc cgtacacggg agtggccaat ggctacgctt
     2221 acgaggccgc ggaggtcgcg cgacgcatca ccgcggggga gaagcagagc cccgcgcaca
     2281 ccccggagat gacgctcgag gtgatggcga ccctcgacga ggtgcgccgc cagatcggcc
     2341 tgacgtaccc gggagagtag tcggcgcggc ggtagcctag gcgtcatggc cctcgcacgc
     2401 gcgcccctcc ctcgcgcaca cctcgatggc gcgcacgtgc ggctcgccgc ggttatcgcg
     2461 gcgggcggcg ccgcgggagc gctcctccgc tggggcatcg gtcgcgagtg gccggtgcta
     2521 ccgggacagt tcccgtggag caccctgtgg atcaatgtga cggggtgcct tgcgatcggg
     2581 gtcctgatgg cgctcatcga ggaggtcctc gcgggccgcg tctacgtccg gcccctgctc
     2641 ggggtggggc tcctcggggg cttcaccacg ttctccacct tcgccgtgga gacgcgcgcg
     2701 ctcctgggcg cgcaccccgc gctcgcgctc gtgtactacc tcggcacccc cgccgccgcc
     2761 gtcctggcga cgatcgtcgg cgccgtcctc gtgacgtccg ccctcaaggc ccggacatgg
     2821 ttcgcctccc gaaggagcat cgcatgacca ccatcgcaag ccccgccctg cggctcaccg
     2881 tcatcgtcga tgaggccgtc caatggcatc accaccccct ctacaccgag atcgtccacc
     2941 gcgcgcatgc ggctggcctg gccggtgcga cggtcattcg cggcgtggag gggtacggcg
     3001 cgtccgggca catccacacc acgcgcatcc tcgcgctctc ggaggccctg ccggtgctga
     3061 tcctgatcat cgacgaggag aagaagatca gggccttcct tcccgaactg gacgagttgg
     3121 ttgtcagggg aatcgtcgtc ctcgacggcg tcgaggtcgt ccggtacgtg ggcgaagcgc
     3181 gggaggcagg gtgatccccg tcgccgtcgc gctcggcgcc gccgttggcg cccctgcccg
     3241 ctacttcacc gatcggtacg tgcaatcgcg caccgcgtcc gtcttcccgt ggggaacgct
     3301 gacggtgaac gccgccgggt cgctcgtcct tggcctgctc gtcggctccg gggcctccgg
     3361 cacgctcgcg gcgctcgccg gggtcggctt ctgcggcgcc ctcaccacct actcgactct
     3421 cggctacgag acgcatcggc tcatcaggga ccgctcgatg ctgctcgcgg cgctcaacgt
     3481 gcttggctcg ctcgtggtgg ggctcgcggc cgcgagcctc gggtactggg tggcgacggc
     3541 ggtggcggca tgaacgggat gcgatcggac gacgcgcaga gcgggggccg gaaggcgagc
     3601 gttccccgcc cgggccgcgc gccgtactcg ccgtggcgct tcgtcgtggc gatgggcttc
     3661 gtcagcatgc tcgcggacgt ggtgtacgag ggctcgcggg cgatcacggg acccttcctc
     3721 gagtcgctcg gggtatccgc agttgcggtc ggcgcgatca cgggggtggg tgaggcgatc
     3781 ggtttcgctg gacgccttgg ctccggtccc ctcgccgaca agacgcgcgc ctattggccc
     3841 ctcttgatcg tcggctacgc gctgaccgtg gttgcggtcc ccttcctcgg cgtgacgtcg
     3901 gtggtgtgga tcgtcgcgac gctcatcttc attgagcggg cgggcaaggc ggtgcggcgg
     3961 cccgccaagg acgtcatcct ctcccacgcg acctcgggga tcgggcgggg tcgtgggttc
     4021 gcggtccacg aggcgctcga tcagttcggc gccgtcctcg ggccgctcgc cgtggcgggc
     4081 gccttccacc tcacgcacaa ctacgcgccg acgttcgcca tgctcgcgct cccaggggcc
     4141 atcgtgatgg tgctacttct gcgcctgcgc aagcgcgtcc cggatccctc ggcctacgag
     4201 gaggccttgg ccgccgagct ggcgatctcc cccccaagcg cgggtcgtcg cgcggcggcc
     4261 cacccgcccc tcgggcgccc ctactggtgg tacctctcct tcgcgaccgt caccacgatc
     4321 gggtactcga cgttcggcgt cctgggttat cacttggtgc acgagggcct agcctcgacc
     4381 gcgaccgttc ccgtcatcta tgcgggagcc atgatcgtgg acgcgctcgc cgcgctggcc
     4441 gtgggggcgc tcttcgaccg cgtcgggagg cgcgtccttc tcgtcgtccc catgctcgcc
     4501 gccgccatcc cgccgctcgc gttcgcgcgg agtctcccgt tggccatcgc gggcatgctc
     4561 gtctggggcg ccgtgatggg gattcaggag tcggttcttc gcgccgcggt cgccgacctc
     4621 attccgtcgg cgcgcagggg gacggcctac ggcatcttcg ccgcggcgat cggcgccgcc
     4681 gccctcgtcg gcggcatcct caccggcggg ctgtacgaga cccaccttcc cctcctcgtg
     4741 gccgtgatcg ccgggatcca ggtggccgcg ctcgtcctct atgtggcctg gtccaggggt
     4801 ctgcggtcgg aggtaccgta ggggcatggc ataccccggc ttcttcttgg tgttcgaggg
     4861 tggcgacggc gtcggcaaga cgacgcaggc aaccatgatt gctcggtggc tcgcctcccc
     4921 cgagggcggc acccgctacg tcacgatcac ccgcgagccg gggggcacgc cactgggcct
     4981 gcagcttcgc gaggccgtca tgcacggggg cgacgtctct ccgcgcgccg aggcgctcct
     5041 ctatgctgcc gaccgcgccc agaacgtcga cgaggtggtc tttcccgcgc ttgagcaggg
     5101 tgacatcgtc gttcaggacc gcttcatcga ctcctcgatc gcctaccagg cggtcgcgcg
     5161 cgggctcggc gaggaaatcg ggcgtatctc ggcgtgggcc gccgacgggc tcatccccga
     5221 cctcacgatc gtcttggaca tggaacccga cgcctcgcgc cttggggagg accttgaccg
     5281 ggtggagcag gacacgtggg cccgggcggg cgagatccgc caggcgttcc tcgacctcgc
     5341 ggagcgcgaa cccgggcgct acctcgtcct cgatgcctcc aggccgctcg aggtggtcca
     5401 ctccgacatt tgttcggctc ttcaagccgt cctcccggag ccgtcgcgcg gcttccgcgc
     5461 ccccaagccg gtcccgcggg tgggcaactg gacgcagtgg gacctctcgg agtgagcgtc
     5521 tgggaaacgg tcgtcgggca gggggacgcg ctcgatcccc tgatcgccgc ggtggagggc
     5581 gatcgcgcga tgacccacgc ctggcttatc acggggccgc ccggctcggg tcgatccatc
     5641 gccgcgcgtg ctttcgccgc ggcgcttcag tgccccagcg ggggctgcgg tgagtgccac
     5701 gcgtgccgaa cggtcctcgc gggcgcgcac cccgacgttc ggatcgtcgc caccgagaag
     5761 gtaaccatca cggtggacga ggtgcgctcg ctcgtcagca acgcgcagag cacgccctcg
     5821 cagggtcgat ggagggtcat catcatcgag gacgccgacc gcatgaccga gcgaacctcc
     5881 aacgtgctcc tcaaggccgt cgaggagccg cccccgagga ccgcgtggat cctgtgcgcg
     5941 ccgagccttc acgacgtggc caccaccatc cgctcgcggt gcagggtggt gacgctccgc
     6001 atcccctccg tcgaggtcgt caccgcgctg ctcgtcgagc gtgacgggat tcagcccgaa
     6061 ctcgcgcgcg agtgcgcgct cgcggcccag tgccacattg gcgtc
//
LOCUS       DASVKP010000107         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1108947, whole genome shotgun sequence.
ACCESSION   DASVKP010000107 DASVKP010000000
VERSION     DASVKP010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108947"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VF362_02275"
     CDS             complement(<1..430)
                     /locus_tag="VF362_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HEX7589784.1"
                     /translation="MTGTDAAVLLLEDGRKFEGRAWGARGETTGEIVFSTGMTGYQET
                     LTDPSYYRQIVVMTAPHIGNTGVNDEDGESSRIWVAGYVVRDPARRASNWRAVRSLDE
                     DLVAQGIVGIAGIDTRELVRILRSAGVMRAGIFSGDALGPE"
     gene            complement(462..>1628)
                     /locus_tag="VF362_02280"
     CDS             complement(462..>1628)
                     /locus_tag="VF362_02280"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX7589785.1"
                     /translation="GAGLVALPGLVDLHTHLREPGREDAETIETGSRAAAAGGYVAVH
                     AMANTTPVADTAGVVEQVWHRGREIGLVDVFPVGAVTVGLAGEHLAELGAMALSAARV
                     RVFSDDGKCVHDPVVMRRALEYVKAFDGVIAQHAQDPRLTEGSQMHEGIVSGELGLQG
                     WPAVAEEAIVARDCLLAEHVGGRVHILHLSTAGSVEIVRWAKARGINVTAEATPHHLF
                     LTDERARGYDPRFKVNPPLRPEADVEAVRAGLADGTIDVVGTDHAPHPDEDKDCEWAT
                     AAFGMIGLETALGIVQKALVEPGLMTWRDVARVMSERPAEIGRVEGHGRPLAVGEPAN
                     VTLVNPKLAWTVNPPAMRSRSHNSPFSGIELPGSVVATILRGTVTYAHDSLGGL"
BASE COUNT          225 a          601 c          547 g          255 t
ORIGIN      
        1 cctcgggtcc cagcgcgtct ccggagaaga tgcccgcgcg catgacccct gcgctacgca
       61 gaatgcgcac caactcacga gtgtcgattc ccgcgatgcc gacgatgccc tgggcgacga
      121 ggtcctcgtc gaggctcctg accgcgcgcc agttggaggc tcgccgcgcg ggatcccgca
      181 ccacatagcc cgccacccaa atccgcgacg actcgccgtc ctcgtcgttc acccccgtgt
      241 tgccgatgtg cggcgcggtc atgacgacga tctggcggta gtacgagggg tcggtcaggg
      301 tttcctggta cccggtcatg ccggtgctga agacgatctc tccggtggtc tcgccgcgcg
      361 cgccccaggc gcgcccctcg aactttcggc cgtcctcaag gaggaggacc gccgcgtcgg
      421 tgcccgtcac gtcggcgtcg gtcaagtcgg cgttggtcaa gtcacagacc cccgagcgag
      481 tcgtgggcgt aggtcacggt tccgcgcagg atcgtcgcca cgacggagcc ggggagttcg
      541 atccccgaaa acggcgagtt gtggctgcgg ctacgcatcg cgggggggtt gaccgtccat
      601 gcgagtttcg gattgacgag cgtgacgttc gcgggctcgc cgaccgccag ggggcggccg
      661 tggccctcca cccttccgat ctcggcgggc cgttcggaca tcacccgcgc gacgtcgcgc
      721 caggtcatca ggccgggctc cacgagcgcc ttctggacga tcccgagggc cgtctcaagc
      781 ccgatcatcc cgaaggccgc ggtcgcccat tcgcagtcct tgtcctcgtc cgggtggggc
      841 gcgtggtccg tccccacgac gtcgatcgtt ccgtccgcca ggccggcccg caccgcctcc
      901 acgtcggcct ccgggcgaag cggcgggttg accttgaatc gcgggtcgta gccgcgcgcc
      961 cgctcatccg tgaggaagag gtggtgcggc gtcgcctcgg ccgtgacgtt gattccccta
     1021 gccttcgccc accggacgat ctcgaccgaa cccgccgtcg agaggtggag gatatggacc
     1081 ctcccgccca cgtgctcggc gagcaggcag tcccgcgcga cgatcgcctc ctccgcaacg
     1141 gccggccacc cctgcaggcc cagctcgccg gacacgattc cctcatgcat ctgcgagccc
     1201 tcggtcaggc gagggtcctg cgcgtgctgc gcgatcacgc cgtcgaaggc cttgacgtac
     1261 tccagggcgc gtcgcatgac gaccgggtcg tggacgcact tgccgtcgtc gctgaagacc
     1321 cgcacgcggg ccgcagagag cgccatcgct ccaagctcgg cgaggtgctc ccccgcgagg
     1381 cccaccgtga cggcgccgac agggaagacg tcgacgaggc cgatttcgcg gcccctgtgc
     1441 cacacctgct cgacgacgcc cgccgtgtca gctaccggag tcgtgttcgc catggcgtgg
     1501 accgcgacgt agccgcctgc cgctgccgcg cgcgaccccg tctcgatcgt ctcggcgtcc
     1561 tcgcggcccg gttcgcgtag gtgcgtgtga aggtccacca ggccgggaag ggcgaccagg
     1621 cccgcgcc
//
LOCUS       DASVKP010000108         4428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_780480, whole genome shotgun sequence.
ACCESSION   DASVKP010000108 DASVKP010000000
VERSION     DASVKP010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780480"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /gene="gndA"
                     /locus_tag="VF362_02285"
     CDS             complement(<1..1059)
                     /gene="gndA"
                     /locus_tag="VF362_02285"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEX7589786.1"
                     /translation="MPKAAIGVTGLGVMGRNLARNFARHGYTVAVHNRHIESMRSLIA
                     DHGDEGTFVGCVSDADFVAALAKPRTIVIMVPAGGPTDSVIDSLVPLLETGDIIVDAG
                     NSHFTDTRRREAALAARGLHFVGMGVSGGEEGALTGPSIMPGGSKASYATLGPILETI
                     AAQVDGVPCCVHVGPDGAGHFVKMVHNGIEYADMQLIAEAYHLLRSGLGLEPAAIGDV
                     FAEWNKGDLESYLIEITAEVLRQKDATTGAPLVDVIVDEAGQKGTGRWTVQAALDLGV
                     TVTAIAEATFARVLSGQRAQRTAARATLTSGASPVQIADRDAFVEDVRPALYASKVVA
                     YAQGFDQIQVASDAYGWAI"
     gene            1162..1725
                     /locus_tag="VF362_02290"
     CDS             1162..1725
                     /locus_tag="VF362_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3000 domain-containing protein"
                     /protein_id="HEX7589787.1"
                     /translation="MSATFSDAPQDFQQVLRSIQGARVRADVALVEAPAPGRIAPYAV
                     AINGEVIGHDGVEGSGRFVVLHDPGGQDSWQGNLRIVALVKASADPEVGSDDMWAEVA
                     WSWISDALTGLPHRALGGTVTKVTDRSFGDLAQTDDAVRVEVRVSWTPLDSDIAPHLR
                     AWADLMAACSGVPPLPEGVTMLPGKSA"
     gene            1722..3026
                     /locus_tag="VF362_02295"
     CDS             1722..3026
                     /locus_tag="VF362_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEX7589788.1"
                     /translation="MTEPKDAAPDASEEAPKPTPLTVPEDGVPPIIDTRDALRAAEKR
                     MAAGEGPVAVDAERASGFRYGQKAYLAQFKRRGAGIALVDTAALTDLSGLSDSFGAAE
                     WVIHAASQDLPGLRELGMEPAAVFDTELASRLLGWPKVGLAAVVERELGLTLAKEHSA
                     QDWSERPLPGAWLNYAALDVEVLLEVRDGLERHLAEAGKLEWAHQEFEAVRTAAPTNA
                     RIDPWRRTASSHLVKDRRGQAIVKALWEAREEAAIQQDTAPGRVLRDQAIVAAAQAKP
                     ESLDALLAIREWQSRGNKRRAPQWYAAIAAAMALPEKQLPDLRGPRADGPPQARMWAD
                     KRPEAAARLDAAKKVVAALMAAHDIPAENLLQPDALRRVCWDYSGGGEAEIVAVLEER
                     SARPWQISLIAAPLAEAFAAAAASATASASATASATADGAPV"
     gene            complement(3023..>4428)
                     /locus_tag="VF362_02300"
     CDS             complement(3023..>4428)
                     /locus_tag="VF362_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX7589789.1"
                     /translation="VSRAGQLGVVSGTGLDVVLARRLQDGDRGGAIRRALAAFPIPAM
                     AARIKDRYFLPEGRVEGAEYAPIPRMAIHQSELAQELSIVGNFVEVWLAKEGHDGLVG
                     VNYLEKLQMATPTAAYGAMLAGVDYVLMGAGIPRNIPSLLNDLAEHKDVAFPIDVVGA
                     APGAFTVKLNPRELVGSAFAMLKRPKFLAIVSAHILATYLARDEHARPDGFVVEGPTA
                     GGHNAPPRGKLVLDETGQPVFGPRDDADISEVAACGLPFWLAGGRGTPEGLLDSEAQG
                     AAGVQVGTLFALSEDSGLDPEIRDTLVAEAREGTLSVRTDRLASPTGFPFKVAVVEGS
                     LSDPEILAARPRLCDIGFLVTAYQVREGKAGYRCPAEPVKIFESKGGNPEETVGRVCL
                     CNALTANVGLPQTREDGYTEKPLVTLGTDLDGCHRLAKLYPGGWSARQALDWILGALP
                     SAPVLPVAFAARAVSLA"
BASE COUNT          684 a         1580 c         1541 g          623 t
ORIGIN      
        1 gatcgcccag ccgtacgcgt cggacgccac ctggatctga tcgaagccct gggcgtacgc
       61 cacgaccttc gaggcgtaga gcgccgggcg cacgtcctcg acgaaggcgt ctcgatcggc
      121 gatctggacg ggcgaggcgc ccgaggtcag ggtcgcgcgc gccgccgtcc gctgcgcgcg
      181 ttgaccggac aggacgcggg caaaggttgc ctccgcgatc gccgtcacgg tgacgccaag
      241 gtcgagggcc gcctgaacgg tccagcgacc ggtccccttc tggcccgcct cgtccacgat
      301 gacgtccacc aggggcgcgc ccgtggtcgc atccttctgg cgcagaacct cggcggtgat
      361 ctcgatgagg tacgactcga ggtcgccctt gttccactcg gcgaacacgt cgccgatcgc
      421 ggccggttcg agcccgagcc ccgaacgaag caggtggtac gcctccgcga tgagttgcat
      481 gtccgcgtat tcgatgccgt tatggaccat cttgacgaag tgccccgcgc cgtccgggcc
      541 cacgtgcacg caacacggaa ccccgtcaac ttgcgcggca atggtctcga ggatcgggcc
      601 gagcgtcgcg tacgacgcct tcgagcctcc tggcatgatc gacggcccgg tgagggcccc
      661 ctcctcgcca cccgagacgc ccatgcccac gaagtggagg ccgcgcgcgg ccagggcggc
      721 ctcgcggcgc cgagtatccg tgaagtggga attgcccgca tcaacgatga tgtcgccggt
      781 ctccagcagg ggcacgagtg agtcgatgac ggaatccgtt ggcccgcccg cagggaccat
      841 gatgacgatg gtccgcggct tcgcgagcgc ggcgacgaag tccgcgtcag acacgcatcc
      901 gacgaacgtc ccctcgtccc catgatcggc gatcagggag cgcatggatt cgatgtgccg
      961 gttgtggacg gcgaccgtgt acccgtggcg cgcgaagttc cgcgccaggt ttcgacccat
     1021 gactccgagt ccggtcactc caatggctgc tttcggcatg cgagaaccct tcggcgctag
     1081 gtaccgagac cagcctacgg cgtccgcgac aaccgcgtgg cgaacctccg agagcccgcg
     1141 gcgcggccct acgctcaagt cgtgagcgcc acgttctccg atgcacctca ggacttccag
     1201 caggtcctgc ggagcattca gggcgcccgc gtccgagcgg acgtcgcgct ggtcgaggcg
     1261 cccgcgcccg gcaggatcgc cccctacgcg gtcgccatca acggcgaagt catcggtcac
     1321 gacggggtcg agggctccgg gcgcttcgtc gtcttgcacg accccggcgg ccaggattcg
     1381 tggcagggga acctccgaat cgtcgccctc gtcaaggcgt ccgccgaccc cgaggtgggt
     1441 tcggacgaca tgtgggccga ggtcgcgtgg tcgtggatct ccgacgcgct gacgggcctg
     1501 ccccatcgcg ccctcggcgg gacggtcacc aaggtgacgg atcggtcgtt cggcgacctc
     1561 gctcagaccg atgacgccgt gcgcgtcgag gtccgggtct cgtggacccc gctcgattcc
     1621 gacatcgccc cgcaccttcg cgcctgggcc gacctgatgg ccgcctgctc gggcgtgcca
     1681 cccctccccg agggcgtcac catgctccca gggaaatccg cgtgacggag ccgaaagacg
     1741 ccgcacccga cgcgagcgaa gaagccccaa aaccgacccc cctgaccgtc cccgaggatg
     1801 gggtcccacc catcatcgac acgcgcgacg cgctacgggc ggccgagaag cgcatggccg
     1861 cgggcgaggg gcctgtcgcc gtggacgccg agcgggcctc gggcttccgg tatgggcaga
     1921 aggcctacct cgcgcagttc aagcgccgcg gagccgggat cgccctcgtt gacaccgccg
     1981 cgctcacgga tctctcgggc ctctcggaca gcttcggcgc cgcggagtgg gtcatccacg
     2041 ccgccagcca ggacctgccg ggtctgaggg aactcggaat ggagcccgcc gcggtgttcg
     2101 acacggagtt ggcctcgcgc ctgctcggct ggcccaaggt cggcctcgcc gccgtggtgg
     2161 aacgcgagct cggcctcacc ctggccaagg agcactcggc tcaggactgg tccgagcgcc
     2221 ccctgcccgg cgcgtggctc aactacgccg cgctcgacgt cgaggtgctc ctcgaggtcc
     2281 gcgacgggct ggagcgccac ctcgccgagg cgggcaaact cgagtgggcc caccaggagt
     2341 tcgaggcggt acgcaccgcc gccccaacga acgctcgcat cgatccttgg cgacggaccg
     2401 cgagctcgca cctggtgaag gatcgccgcg gccaggcgat cgtcaaggcg ctgtgggaag
     2461 cgcgcgaaga ggccgccatc caacaagaca ccgcccccgg gcgggtgctt cgcgaccagg
     2521 ccatcgtcgc cgccgcccag gcgaagccgg agtcgctcga cgcgctcctg gccatccggg
     2581 agtggcagtc gcgcggcaac aagcggcgcg cgccgcagtg gtacgccgcc atcgcggccg
     2641 ccatggccct gccggagaag caacttcccg atcttcgcgg cccgcgcgcc gacggccccc
     2701 ctcaggcgcg gatgtgggca gacaagaggc cggaagcggc ggcccggctc gacgcggcca
     2761 agaaggtcgt cgcggcgctg atggccgcgc acgacattcc cgcggagaac ctcctccagc
     2821 ccgacgcgct gaggcgcgtg tgttgggact actcgggcgg aggcgaggcg gagatcgtcg
     2881 ccgtcctcga ggaacgctcg gcgcgtccgt ggcagatctc tctgatcgcc gcacccctcg
     2941 cggaggcgtt cgccgcggct gcggcatcgg caacggcatc ggcatcggca acggcctcgg
     3001 caacggccga cggcgcgccg gtctaagcga gcgaaacggc ccgcgcggcg aacgcgacgg
     3061 gcaggacagg tgcgctcggc agtgcgccga ggatccaatc gagcgcctgg cgcgccgacc
     3121 aaccgccggg gtacaacttc gcgaggcggt ggcacccgtc gaggtccgtg ccgagcgtga
     3181 cgagcggctt ctcggtgtag ccgtcttcgc gagtctgggg caggccgacg ttcgcggtga
     3241 gcgcgttgca caggcagacg cgccctacgg tctcctcggg gttgccgccc ttgctttcga
     3301 agatcttcac gggctccgcg gggcagcgat accccgcctt cccctcccgc acctggtacg
     3361 cggtgacgag aaagccaatg tcgcacagcc gtggccgcgc ggcgaggatc tcggggtcgg
     3421 acaagctacc ctcgaccacc gccaccttga aggggaagcc cgtcggcgaa gcaagccggt
     3481 ccgtcctcac cgaaagcgtg ccctcgcggg cctcggcgac gagggtgtca cgaatctccg
     3541 ggtcgaggcc ggagtcctcg gacagggcga acagcgttcc cacctggacg ccggcggcgc
     3601 cctgagcctc ggagtcgagc agaccttccg gcgtgccgcg gccacccgca agccagaacg
     3661 gcaacccgca cgccgcaacc tcggagatgt cggcgtcgtc gcgggggccg aagaccggct
     3721 gaccggtctc gtcaagcacg agtttgccgc gcggcggcgc gttgtggccg cccgcggtgg
     3781 ggccctcgac gacgaaaccg tcgggccgcg cgtgctcgtc gcgcgcgagg tacgtcgcga
     3841 ggatgtgggc ggagacgatc gcgaggaact tggggcgctt gagcatggcg aacgcggagc
     3901 cgacgagctc tctggggttg agcttcacgg tgaaggcccc cggtgcggct cccacgacgt
     3961 cgatggggaa cgccacgtcc ttgtgctcgg ccaggtcgtt tagcaggctc gggatgttgc
     4021 gcggaatgcc cgcccccatc agcacgtagt ccactcccgc gagcatcgct ccgtacgcgg
     4081 cggtgggggt cgccatctgc aacttctcca ggtagttgac gccgactagg ccgtcgtggc
     4141 cctccttggc gagccacacc tcgacgaagt tgccgacgat ggacagttcc tgggccaact
     4201 ccgactggtg gatggccatc ctgggaatcg gcgcgtactc cgcgccctcg acccggccct
     4261 ccggaaggaa gtagcggtcc ttgattcgcg ccgccatcgc gggaatcggg aaggcggcga
     4321 gcgcccgtcg aatggcgccg ccgcggtcac catcctggag cctgcgcgcg aggacgacgt
     4381 cgaggccggt cccggatacc accccgagtt gtcccgcgcg cgagacct
//
LOCUS       DASVKP010000109         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_533995, whole genome shotgun sequence.
ACCESSION   DASVKP010000109 DASVKP010000000
VERSION     DASVKP010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533995"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..660
                     /locus_tag="VF362_02305"
     CDS             91..660
                     /locus_tag="VF362_02305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7589790.1"
                     /translation="MRVILATEPAHLEAALALRFAVFVGEQGVSPEGERDALDQAPDT
                     VHFLALSGGGPCFGGPGDVVGVGRLLGPRADGHPHIGRVAIARDRRRSGIGRALMDAI
                     EREALARFGEGGAVTVALSAQVQAIPFYEAIGYVIAGEPYLDEGIWHRDGTKVVRDPE
                     ADGSGPSGTAPRDFARRAPGQRRRRTPQA"
     gene            671..>2057
                     /gene="dnaE"
                     /locus_tag="VF362_02310"
     CDS             671..>2057
                     /gene="dnaE"
                     /locus_tag="VF362_02310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX7589791.1"
                     /translation="MPPSEFVHLHVHTDYSMLDGASRVDDLFAEAERLGQTAMGITDH
                     GFLFGAYDFWAKAIKAGIKPIIGLEAYMTPGTARADRTRVKWGDQTQSADDVSATGAY
                     THTTLWARDNTGLHNLFRMGSLASLEGQFYKPRIDRDLLNRYSGGLIGATGCPSGEVQ
                     TRMRLGQYDAALKAAGEMQDIFGKENYYVEVMDHGAEIERRVVADLIRLKKDLGAPYV
                     ATNDSHYTKAGDREAQEALLCIQSGTTLADEGRFKFDGHSYHLRDSEDMRRTFADFEG
                     ACDNALVIAERCEVSFDTSADYMPRFDCPPGEDEHSWFVKEVERGLRERYPAGVPTPV
                     RERAEFEIDVITQKGYAGYYLVVADFVRWARANGIRVGPGRGSGAGSMAAYAMRITDL
                     DPLVHGLFFERFLNPERETKPDFDIDFDERRRGEVIRYVSDKYGEDKVAQIVTYGTIK
                     AKQALKDAARVL"
BASE COUNT          340 a          690 c          722 g          305 t
ORIGIN      
        1 cgcccacccg gtctctggaa cgcggatcga gttcgagagt ccgtaccccc tggatctcgc
       61 ccgcgcgctt gaggccctga ggggatagcg atgcgcgtca tcctcgcgac ggagcccgcc
      121 caccttgagg cggcgctcgc gctgaggttc gccgtcttcg ttggcgagca gggcgtcagc
      181 cccgaggggg agcgcgatgc cctcgatcag gcgcccgaca ccgtgcactt cctggccctt
      241 tccggcggag ggccgtgctt cggcggaccc ggagacgtcg tcggagttgg ccggctgctg
      301 ggtccgaggg ccgacggcca cccccacatc gggcgcgtcg cgatcgcgcg cgatcggcgc
      361 cgaagcggca tcggccgcgc gctgatggac gcgattgagc gcgaggccct cgcgcgcttc
      421 ggcgagggcg gcgcggtcac ggtcgcgctc agcgcccagg tgcaggcgat cccgttctac
      481 gaggcgatcg gctacgtcat cgcgggggag ccgtacctcg acgaggggat ctggcatcgc
      541 gacggaacca aggtcgtgcg cgacccggag gccgatggga gcggcccgtc gggaaccgcg
      601 ccgcgcgact ttgctcggcg cgcgccgggc cagcgtcgtc gccgcacacc ccaagcctag
      661 aatcgggtcc atgcccccga gcgagttcgt ccacctgcat gtgcacacgg actactcgat
      721 gctcgacggc gcgtctcgcg tggacgacct ctttgccgag gccgagcgcc tgggtcaaac
      781 cgccatgggg atcacggacc acggattcct gttcggcgcc tacgacttct gggcgaaggc
      841 catcaaggcg ggcatcaagc ccatcatcgg gctcgaggcc tacatgaccc cggggacggc
      901 acgtgcggac cgcacgaggg tcaaatgggg cgaccagacg cagtcggccg acgacgtctc
      961 cgcgaccggc gcgtacacgc acacgacgct ttgggcacgc gacaacaccg ggctccacaa
     1021 cctgttccgc atgggatcgc tagcgtcgct tgagggccag ttctacaagc cccgaatcga
     1081 tcgggacctt ctcaatcgat actccggggg cctgatcggc gcgacggggt gcccctcggg
     1141 cgaggttcag actcggatgc gcctcgggca gtacgacgcc gcgctcaagg ctgcgggcga
     1201 gatgcaggac atcttcggca aggagaacta ctacgtcgag gtgatggacc acggggccga
     1261 gatcgagcgt cgcgtcgtcg cggacctcat tcggctcaag aaggacctcg gagcccccta
     1321 cgtggccacg aacgattcgc actacacgaa ggcgggggac agggaggcgc aagaggctct
     1381 cctgtgcatc cagtcgggga cgacgctcgc cgacgaggga cgcttcaagt tcgacgggca
     1441 ctcgtaccac ctccgcgact cggaggacat gcgacggacc ttcgcggact ttgagggggc
     1501 gtgcgacaac gcgctggtga tcgccgagcg gtgcgaggtc tcgttcgaca cgagcgcgga
     1561 ctacatgcct cgcttcgact gcccgcccgg cgaggacgag cactcctggt tcgtcaagga
     1621 ggtcgagcgg ggactccgcg agcgataccc cgcgggggtc ccgacgcccg tgcgcgagcg
     1681 cgcggagttc gagatcgacg tcatcaccca gaaggggtac gcgggctact acctcgtcgt
     1741 tgcggacttc gtgcgctggg cgagggcgaa cggcatccgc gtcggcccgg gccgcgggtc
     1801 gggcgctggc tccatggcgg cctacgcgat gcggatcacg gacctcgatc ccctcgtcca
     1861 cggcctgttc ttcgagcggt tcctcaaccc cgagcgggag accaagccgg acttcgatat
     1921 cgacttcgac gagcgtcggc gcggggaggt cattcgttac gtctcggaca agtacggcga
     1981 ggacaaggtc gcccagatcg tcacctacgg aacgatcaag gccaagcagg cgctcaagga
     2041 tgcggcacgg gttctcg
//
LOCUS       DASVKP010000110         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_700463, whole genome shotgun sequence.
ACCESSION   DASVKP010000110 DASVKP010000000
VERSION     DASVKP010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700463"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VF362_02315"
     CDS             <1..652
                     /locus_tag="VF362_02315"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX7589792.1"
                     /translation="LVESTGGLRLLWAVAKSNHESPRMLARAMVAIHQIDADPIATTL
                     AFPKVVGPVLDWLFSRASAHSDRDLVDRAQRLLATRPAYRGAVLCHGDLHPLNILRSP
                     GRDTIVDWTHAQYDDPLYDVAFASTAMTLIPVPAPAFLRPLIAAVGARQARRFTREYE
                     RASKTQVDRERLDWFVRVIALRIRVEAAEWRRLHPFDDPDGNSPQRYVNLFPRYGL"
     gene            701..1360
                     /gene="rsmD"
                     /locus_tag="VF362_02320"
     CDS             701..1360
                     /gene="rsmD"
                     /locus_tag="VF362_02320"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX7589793.1"
                     /translation="MTRIIAGTLGGRLLAVPPKGTRPTTDRVREAIFSRLDHEKSLRG
                     AWVLDAFAGSGALGIEALSRGAKHAVFVESNSAAVAVIRANAESLGVTKATEVARDKV
                     LPWLGRSTRPVDLATFDLALLDPPYDLPPEELAATLAALVPRLTEGAVVVVEWSSRSE
                     APAWPKGLVPTGAKRYGETTVHYAVASGAALSGAALSGDDASGEDASAEETPPSDSLD
                     T"
     gene            1357..1842
                     /gene="coaD"
                     /locus_tag="VF362_02325"
     CDS             1357..1842
                     /gene="coaD"
                     /locus_tag="VF362_02325"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX7589794.1"
                     /translation="MRIAVFPGTFDPITVGHVDIAIRARTLFDHVVIAVAHNARKNPV
                     LDTHTRVALARDALANLEGVEVKPSEGLLVDFCKAEGAIAIVKGLRGGADYDFERSMA
                     LMNRALTGIETVFISGDPALQHVASSLVRDVARHGGSIAGLVPEGVEPALLAALERER
                     D"
     gene            1957..>2312
                     /locus_tag="VF362_02330"
     CDS             1957..>2312
                     /locus_tag="VF362_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX7589795.1"
                     /translation="MATSRGPAASPFVVSVRDLARRPGSQLSISRSIPAPAVFGTDVI
                     GVPEGSDLTLELSMESISEGIWVSGTVAARAVGECSRCLDEVSEDVLAPVQGLFAHPG
                     AESAGEGDDVDVRDFDG"
BASE COUNT          322 a          822 c          795 g          373 t
ORIGIN      
        1 gctcgtcgag tcgaccgggg ggctccggct cctctgggcg gtcgccaaga gcaaccacga
       61 gagcccgagg atgctcgcgc gcgccatggt cgcgatccac cagatcgatg ccgatcccat
      121 cgcgacgacg ctcgcattcc ccaaggtcgt cggtccggtt ctcgattggc tcttctcacg
      181 ggcatccgcc cactcggatc gtgacctggt tgaccgggcg cagcgactcc tcgccaccag
      241 gcccgcgtat cggggtgcgg tgttgtgtca cggggacctt caccccctca acattttgcg
      301 cagccccggt cgcgacacga tcgtcgactg gacccacgcg cagtacgacg acccgctcta
      361 cgacgtggcg ttcgcgtcca ccgcgatgac cctcatcccg gtgccggccc ccgccttcct
      421 gcgcccgctg atcgcggccg tcggggcgcg ccaggcccgg aggttcaccc gcgaatacga
      481 gcgcgcatcg aagacccagg ttgaccgtga gcggctcgac tggttcgtgc gggtcatcgc
      541 gctgcggatt cgcgtcgagg ccgcggagtg gagacgtctc cacccgttcg acgacccgga
      601 cggcaatagc ccgcagaggt acgtcaactt gttcccgcgc tacgggctgt agcgcacggg
      661 cggggcttgc cgtcgcgcgg cagtcccgta gcctgggcgc gtgacgcgca tcatcgcggg
      721 aaccctgggc ggccgccttt tggcggtgcc gcccaaggga acgcggccaa ccacggaccg
      781 ggtccgcgag gccatcttca gccgcctcga ccacgagaaa tcgcttcggg gcgcatgggt
      841 gctcgacgcg ttcgcgggct cgggggcgct cggcatcgag gcgctctcgc gcggggcgaa
      901 gcacgccgtc ttcgtcgagt cgaactccgc tgcggtcgcg gtgattcgcg cgaacgcgga
      961 gtcccttggc gtcacgaagg cgaccgaggt cgcccgcgac aaggtccttc cgtggcttgg
     1021 gcgctccacg cgccccgtgg acctcgcgac gttcgacctc gccctcctcg acccgccgta
     1081 cgaccttccg cccgaggaac tcgccgcgac gctcgccgcg ctcgtgccgc gcctgacgga
     1141 aggggccgtc gtcgtcgtcg aatggtcctc ccggtcggaa gcgcccgcgt ggcccaaggg
     1201 cctcgtgccg acgggggcca agcgctacgg cgagacgacg gtccactacg ccgtcgcgtc
     1261 gggcgccgcc ttgtcgggcg ccgccttgtc gggcgacgat gcgtcgggtg aggatgcgtc
     1321 tgccgaggag accccaccga gcgatagtct cgacacatga gaatcgcggt cttcccagga
     1381 acgttcgatc cgatcacggt cgggcatgtg gacatcgcca tccgggcccg gaccttgttt
     1441 gatcacgtgg tcatcgccgt ggcgcacaac gcccgcaaga acccggtgct cgacacccac
     1501 acgcgcgtcg ccctcgcccg cgatgcgctc gcgaatctcg agggcgtcga ggtgaagccc
     1561 tctgagggtc tcctcgtgga cttctgcaag gcggagggcg cgatcgcgat cgtgaagggc
     1621 ctccgcggcg gcgcggacta cgacttcgag cgttccatgg ccctgatgaa ccgcgcgctg
     1681 accggcatcg agacggtgtt catctccggc gaccccgcgc tccagcacgt cgcctcgtcc
     1741 ctcgtgaggg acgtcgcgcg ccacgggggc tcgatcgctg gcctcgttcc cgagggcgtc
     1801 gaacccgcgc tccttgcggc gctcgagaga gagcgcgact agatcgagcc tcgccggttg
     1861 agtcggatgc cgcccatcac gtagactcat ccgctggtcc agccgctcgt ggcgcgggcc
     1921 aaggtccccg aggaccggag atagcaggag gatgcgatgg cgacgtcgcg tggccctgcg
     1981 gcctccccgt tcgtcgtctc cgttcgagac ctcgcgcggc gaccaggctc gcagttgtcg
     2041 atctcccgca gcatcccggc acccgcggtc tttggcaccg acgtgatcgg cgtgcccgag
     2101 ggttccgacc tcacgctcga gttgagcatg gagtcgattt ccgagggcat ttgggtctct
     2161 ggcacggtcg cggctcgcgc cgtgggggag tgctcgcggt gcctcgacga ggtgagcgag
     2221 gacgtcctcg cccccgtcca gggactgttc gcccatcccg gcgcggaaag cgcgggggag
     2281 ggcgacgacg tggacgtgcg ggacttcgac gg
//
LOCUS       DASVKP010000111         4515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1028590, whole genome shotgun sequence.
ACCESSION   DASVKP010000111 DASVKP010000000
VERSION     DASVKP010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028590"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VF362_02335"
     CDS             complement(<1..613)
                     /locus_tag="VF362_02335"
                     /inference="COORDINATES: protein motif:HMM:NF022794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HEX7589796.1"
                     /translation="MRLLRRGGPILTGRRIPGRIVIVAIVLLAFGVGVGAGWGSAVMA
                     GFLVSAIPGPEPSTSPSASTSGQPNTDIPVNLYPALTRFMNSDDRDAGLTSLDIPVRG
                     DGNLTVVPGKSDPVGTGPTRWVRIEIEDGVPIDPDIVTSYVMSILGDAQGWTAHGRVS
                     FGRTDGVADVRIVFASPKTAESLCPRPHDPATVDIAPPDVPGAV"
     gene            663..881
                     /locus_tag="VF362_02340"
     CDS             663..881
                     /locus_tag="VF362_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HEX7589797.1"
                     /translation="MEIKIGVQNISREITLETDETADAVAKAIADGMAGATIEFHDAK
                     GRRVMVPSAALGYVEMGSEEKRRVGFGG"
     gene            1037..2653
                     /locus_tag="VF362_02345"
     CDS             1037..2653
                     /locus_tag="VF362_02345"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7589798.1"
                     /translation="MSETETTTLAPTRTFADFGVGAPIVEALAAVGIVYPFPIQEMTL
                     PVALNGHDIIGQAKTGTGKTLGFGIPLLEHLSGPGEPGWDVLTAPGKPQALVVAPTRE
                     LAIQVAQDLDVAAKFRSARVLQVYGGRAYEPQIEALKRGVEVVVGTPGRMIDLMKQGH
                     LDLRYVRTVVLDEADEMLDLGFLPDVEKLLAGTPASRHTMLFSATMPGAVVALARRYM
                     IQPTHIRANDPEDSGATVKAIKQFVYRAHAMDKIEVLARILQAKDRGLTIVFARTKRT
                     TAKVADELAARGFASGAIHGDLGQGAREQALRAFKSGKIDVLVATDVAARGIDVDDVT
                     HVINYQCPEDEKIYLHRTGRTGRAGKTGVAVTFVDWDDMPRWGLIDKGLDLGYPEPEE
                     TYSNSPHLYEEMEIPEGTTGTLPRANRTREGLGAETLEDLGETGKKPGGGKSGRSGGS
                     DRGGSDRGGSDRGGHGRSGGSGGPGSGGSARRSGGGFGGGSGSGGGRSQSRPPRSEPS
                     GSKAESPSDRPSGARGSRGRKRTGGGTPAA"
     gene            complement(2657..3481)
                     /locus_tag="VF362_02350"
     CDS             complement(2657..3481)
                     /locus_tag="VF362_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX7589799.1"
                     /translation="MRIDLHTHSTMSDGTEPPAQVMRAAAAAGLDVVALTDHDTLAGW
                     DEAEAEAGALGLHFVPGVEISSRHRGVSVHLLAYWPRVDDADLLAMMARTRGARVDRA
                     REIVSRVARDYPITWEAVSAHSESAETVGRPHIADALVAAGLVSDRDAAFSEILHDGS
                     PYYVPHYAPDVLDVVRAIRAAGGVSAFAHPGADGRGRVVPTSVIEAMARAGLVGVEVE
                     HRDHSEGQRERLARIAERLGLVPLGASDYHGAGKPNRLGENMTSPEALAALEAARA"
     gene            3586..>4515
                     /locus_tag="VF362_02355"
     CDS             3586..>4515
                     /locus_tag="VF362_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEX7589800.1"
                     /translation="MTEEAKGTMSASRAQRPDSDAFKRFITEQWGGDERGRATRAAGA
                     AFAAERRARLSSQFPGTRIVAPAGDLSPRSNDTDYRFRPDSDFAYLTGLGVDHEPGAV
                     LVLEPTAAGHLATLYLNPPAGRDGEGFYSDPRLGEFWIGKRPGLAEFAAMTGIPTLPL
                     AELPQGIRLASQPSRAVARALAMMRIVKDAYEVDQVRAAVAATIAGFERVVGEFPRAM
                     EHKRGERVIEATFDGHAREEGNAVGYETIAASGPHATTLHWIRNDGQVREGDLLLLDA
                     GVEVESLYTADITRTLPVGGRFTDVQRRVYDVVL"
BASE COUNT          716 a         1592 c         1598 g          609 t
ORIGIN      
        1 gtactgcgcc gggaacgtcc gggggcgcga tgtccacggt cgccgggtcg tgcgggcggg
       61 gacacaggct ctccgccgtc ttgggggacg cgaagacgat gcggacgtcg gcgacgccgt
      121 ccgtcctgcc aaacgacacc ctgccgtgag cggtccatcc ctgcgcgtcg ccaaggatcg
      181 acatcacgta cgacgtgacg atgtcgggat cgatcgggac gccgtcttcg atctcgatcc
      241 ggacccaccg cgtcgggccc gtgccgacgg ggtcgctctt tcccggtacg accgtgagat
      301 tcccgtcgcc gcgcacgggg atgtcgaggc tcgtcagccc cgcgtcgcgg tcgtcggagt
      361 tcatgaagcg cgtcagcgcg gggtagaggt tgaccgggat gtcggtgttg ggctgcccgg
      421 aggtggacgc cgaggggctc gtcgacggtt cggggccggg gatcgcgctc acgaggaagc
      481 ccgccatgac ggcgctcccc caacccgcgc cgaccccgac cccgaacgcg agaagcacga
      541 ttgcgacgat cacgatcctg ccggggatgc gcctgcccgt gaggatcggc ccgccacgtc
      601 gcagcaatcg catggctcta cgatagttcg taggacaccg ttacccgatt ccggaggcga
      661 gcatggagat caagatcggc gtgcagaaca tttcgcgcga gatcacgctc gaaacggacg
      721 agacggccga cgccgtggcg aaggccatcg cggatggcat ggcaggcgcg acgatcgagt
      781 tccacgacgc caagggccgc cgggtgatgg tgccgtccgc ggccctcgga tacgtcgaaa
      841 tggggagcga agagaagcgc agggtgggct tcgggggctg agaagggggc ccggcgcccc
      901 cggaaggtac actggaggcg actcgaggtt gcccggtcgt cacgtgaagc gcggcaaagg
      961 ccgcatggag attcgcgatc ggctacccgc cgcaaggcgc cgcgcccggc gccaccgaat
     1021 ctaggaacct tcacccatga gtgagacaga gaccaccacg ctcgcgccta cccgcacgtt
     1081 tgcggacttt ggcgtcggcg cacccatcgt cgaggcgctc gccgccgtcg gcatcgtcta
     1141 cccctttccg atccaggaga tgaccctccc ggtcgccctc aacggtcacg acatcatcgg
     1201 ccaggccaag accgggacgg ggaagacgct cggcttcggg atcccgttgc tcgagcacct
     1261 ctccggaccc ggggagcccg ggtgggacgt cctcaccgct ccgggcaagc cccaggccct
     1321 cgtcgtcgcc ccgacgcggg agctcgccat ccaggttgcg caggacctcg acgtcgcggc
     1381 gaagttccga tccgcacgcg tcctgcaggt ctacggaggc cgcgcgtacg agccgcagat
     1441 cgaagcgctc aagcggggcg tcgaggtcgt cgtcggaacg ccggggcgca tgatcgacct
     1501 gatgaagcag gggcacctcg acctgcgcta cgtccggacc gtcgtgctcg acgaggcgga
     1561 cgagatgctc gaccttggct tccttcccga cgtcgagaaa ctcctcgcgg gcacgcccgc
     1621 gtcgcgccat acgatgctct tctcggcgac catgccgggc gcggtcgtcg cgctcgcgcg
     1681 ccggtacatg atccagccga cccacatccg ggcgaacgac cccgaggact ccggggccac
     1741 cgtcaaggcg atcaagcagt tcgtgtaccg cgcgcacgcg atggacaaga tcgaggtgct
     1801 ggcccggatc ctccaggcga aggaccgggg cctcaccatc gtgttcgcgc gcaccaagcg
     1861 caccaccgcg aaggtcgccg acgaactcgc cgcgcgcggc ttcgcgtccg gcgccatcca
     1921 cggcgacctg ggccagggcg cgcgcgagca ggcgctccgc gccttcaagt cgggcaagat
     1981 cgacgtgctc gtcgccacgg acgtcgccgc ccgcggaatc gacgtcgacg acgtgacgca
     2041 tgtgatcaac taccagtgcc ccgaggacga gaagatctac ctccaccgca cgggccgcac
     2101 gggccgcgct ggcaagaccg gcgtcgccgt caccttcgtg gactgggacg acatgccgag
     2161 gtgggggctg atcgacaagg ggctcgacct cggctacccc gagccggaag agacctactc
     2221 gaactcgccg cacctctacg aggagatgga gatccccgag ggcaccaccg gaacgttgcc
     2281 ccgggccaac cgcacccgcg agggtctggg cgcggagacg cttgaggacc tcggagagac
     2341 gggcaagaag cccggcggcg gcaagtccgg caggtcgggc ggttccgaca ggggcggttc
     2401 cgaccggggc ggttccgacc ggggcggcca cgggcgttcc ggcggatccg gcggccccgg
     2461 ctcagggggc tcggctcgtc gctcgggtgg tggtttcggc ggaggctctg gttccggcgg
     2521 aggcaggtcg cagtctcgcc ctccgcggtc ggaaccctcg ggctcgaagg ctgagtcacc
     2581 gtccgacagg ccgagcggcg ctcgcgggag tcgcggcagg aagcgcacgg gcggcgggac
     2641 tcccgccgcc taacacctag gcgcgcgcgg cctccagggc cgcgagcgcc tccggggagg
     2701 tcatgttctc gccgaggcgg ttcggtttcc ccgcgccatg atagtcgctc gcgccgaggg
     2761 ggacgaggcc gaggcgctcg gcgatgcggg cgagtcgctc gcgctggccc tcggagtgat
     2821 cacggtgctc cacctcgacg cccacgagtc ccgcgcgcgc cattgcctcg atcacggacg
     2881 tcggaaccac ccggccgcga ccgtcggcgc cggggtgggc gaacgccgag accccgcccg
     2941 ccgcgcggat cgcccggacc acgtcgagca cgtccggtgc gtagtgggga acgtagtacg
     3001 gcgaaccgtc gtgcaggatc tccgagaacg ccgcatcccg atcgctcacg agaccggccg
     3061 cgacaagggc gtcggcgatg tgggggcggc cgaccgtctc cgccgactcc gagtgcgccg
     3121 agaccgcctc ccacgtgatc gggtagtcgc gagcgactct gctgacgatc tcccgcgcgc
     3181 ggtcgacgcg agcgccccgc gtgcgggcca tcatcgcgag gaggtcggca tcgtcgacgc
     3241 gcggccaata cgcgagaagg tgcacgctca cgccccgatg cctgctggag atctcgacgc
     3301 ccggcacgaa gtgaaggccc agcgcgccag cctcggcctc cgcctcgtcc caccccgcca
     3361 acgtgtcgtg gtccgtgagc gcgacaacat cgagccccgc cgcagcagca gctcgcatca
     3421 cctgggcggg cggttccgtc ccgtcggaca tcgtggagtg ggtgtggagg tcgatgcgca
     3481 cgacgccagg ctacgccgcg gcccgcgccc gaacggaacc ggccagaggg accccgtcgc
     3541 ggcgtcctcg acgacgacac cgcggtcgcg tgggagcatg gtcgggtgac agaagaagcg
     3601 aagggcacca tgagcgcatc ccgcgcgcag cggcccgatt ccgacgcgtt caagcggttc
     3661 atcaccgagc agtggggcgg ggacgagcgc ggtcgcgcca cccgggcggc gggcgccgcc
     3721 ttcgccgccg aacgacgcgc gcgcctctcc tcgcagttcc ccggcacccg catcgtcgca
     3781 ccggcgggcg acctctcgcc ccggtcgaat gacacggact accgcttccg accggattcg
     3841 gatttcgcct acctcacggg cctcggagtg gatcacgagc cgggagccgt cctggtcttg
     3901 gagccgacgg cggccgggca tttggcgacc ctgtacctca atccccccgc gggccgcgac
     3961 ggcgagggct tctactccga ccctcgcctc ggcgagttct ggatcggaaa gcgccccggc
     4021 ctcgccgagt tcgccgcgat gacggggatc ccgacccttc ccctcgcgga actgccgcag
     4081 gggattcggc ttgcctccca gccgtctcgc gccgtcgcgc gcgccctcgc gatgatgcgc
     4141 atagtcaagg acgcgtacga ggtggaccag gtgcgcgccg ccgtcgccgc gacgatcgcg
     4201 gggttcgagc gcgtggtggg cgagtttccc agggcgatgg aacacaagcg cggcgagcgc
     4261 gtgatcgagg cgacgttcga cgggcacgcg cgcgaagagg gcaacgcggt gggatatgag
     4321 acgatcgccg cctcgggacc ccatgcgacg acgctgcact ggatccgcaa cgacggacaa
     4381 gtgcgtgagg gcgacctcct tctcctcgac gcgggcgtcg aggtcgagag cctctacacc
     4441 gcagacatca cgcggacgct gcccgtcggg ggccgattca cggacgttca gcgccgcgtc
     4501 tatgacgtcg tcctt
//
LOCUS       DASVKP010000112         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_863889, whole genome shotgun sequence.
ACCESSION   DASVKP010000112 DASVKP010000000
VERSION     DASVKP010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863889"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..1829
                     /gene="lepA"
                     /locus_tag="VF362_02360"
                     /pseudo
     CDS             21..1829
                     /gene="lepA"
                     /locus_tag="VF362_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142589.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
     gene            1832..>2310
                     /locus_tag="VF362_02365"
     CDS             1832..>2310
                     /locus_tag="VF362_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7589801.1"
                     /translation="MASVVARVAPASGRDFGVYVHVPFCSVRCGYCDFNTYAPGELAG
                     ASREGYVEAALGEMMLARRVMGADPRHASTVFFGGGTPTMLEPAALARLLDGVRGTWG
                     LAEGAEVTTEANPDSVDEAALAALADAGFTRVSLGMQSAVPHVLQTLDRTHRPENVM"
BASE COUNT          379 a          774 c          792 g          365 t
ORIGIN      
        1 cgcgaaccgc gcagggtctc atccggaatt tctgcatcat cgcgcacatt gaccatggca
       61 agtcgacgct ggccgaccgg atgctccagt tgacgggagt ggtggaactt cgggacatgc
      121 gtgcccagta cctcgatcgg atggacatcg agcgcgagcg gggtatcacc atcaagtccc
      181 aggccgtgcg cctgccgtgg atggtggacg gcgagccgtt cgccctgaac atgatcgaca
      241 ctccgggcca cgtggacttc acctaagagg tctcccgaag cctcgcggcg tgcgaggggg
      301 ccgtcctgct cgtcgatgcc gcgcagggga tcgaggcgca gacgctcgcc aacctgtacc
      361 tcgcgatgga gaacgacctc acgatcgtgc ccgtcctgaa caagatcgac ctgcccgcgg
      421 cagagcccga ccggcacgcg gccgaactcg ccggcctcgt tggttgcgac ccgaatgacg
      481 tcatgcgggt gagcgggaag accggcgacg gggtcacgga gttgctcgac cgcatcgtcc
      541 gcgacgtccc cgcgcccgtc ggcgatccca aggcgcccac gcgtgccatc atcttcgact
      601 ccgtttacga cacctatcgc ggcgtcgtga cctacgtgcg cgtcgtcgac gggcacctga
      661 gcccgcgcga gaagatcgtc atgatgtcta ctcgggcgac gcacgagctc ctcgagattg
      721 gcgtcatctc gcccgagccc gtcgcgacca aggggcttgg cgtcggcgag gtcgggtatc
      781 tcatcacggg ggtgaaggac gtccgccagt cgcgcgtcgg cgataccgtg accaacgcgt
      841 cgcacccggc gaaagcgccg atcggcgcgg ttcgcgaccc acgcccgatg gtgttctcgg
      901 gcctgttccc cctcgacggc agcgacttcc ccgtcttgcg cgacgcgctc gatcggctca
      961 agctcaatga cgcggcgctc gtgtacgagc ccgagaattc cgtcgcgctc ggcttcggct
     1021 tccggtgcgg gtatctcggg ctgttgcacc tcgagatcgt cagggagcgg ctcgagcggg
     1081 agttcggcct cgacctgatc tccacggccc cgaacgtcgt ctaccgcgtg acgatggacg
     1141 atggcaagga gatgatcgtc acgaacccga gcgagttccc cggtgggaag atcgccgagg
     1201 ttcgcgagcc cgtcgttcgc gcgacgatcc tgtcgccgag cgagttcatt gggacggtca
     1261 tggagttgtg ccagcagcgg cgcggcacga tgggctcgat gaactacctg acctccgatc
     1321 gcgtcgagct tcactacacg cttccgctcg cggagatcgt cttcgacttc ttcgatcagc
     1381 tcaagtcgcg cacgcgcggc tacgcctccc tcgactacga gccgagcggc gaggaggctg
     1441 ccgcgctggt gaaggtggac atcctgctcc agggggaggc ggtcgacgcc ttcagcgcgg
     1501 tggtccacaa ggacgcggcg tacgcctacg gcctgaggat gaccgcgaag ctccgggagc
     1561 tgatcccgcg ccagcagttc gaggtgccca tccaggcggc gatcggcgcg cgcatcatcg
     1621 cgcgcgagac gattcgcgcc ctgcgcaagg acgtgctcgc caagtgctac ggcggcgaca
     1681 tctcccgcaa gcgcaagctc ctggagaagc agaaggaggg caagaagcgg atgaagacca
     1741 tcggccgcgt cgaggtcccc caggaggcgt tcatcgccgc gctctcgacg ggcgacgagg
     1801 gctcggacaa gggcgagaag aagaagtagc gatggcctcc gtggtggctc gggtggcgcc
     1861 ggcaagcggg cgcgacttcg gcgtctacgt ccacgttccc ttctgctccg tgaggtgcgg
     1921 gtactgcgac ttcaacacct acgcccccgg cgagctcgcg ggtgcgtctc gcgagggtta
     1981 cgtcgaggcc gcgctggggg agatgatgct cgcccgccgc gtgatgggcg cggacccccg
     2041 gcacgcctcc acggtcttct tcggcggcgg cacgccgacg atgctcgaac ccgccgcgct
     2101 cgcgcggctg ctcgacggtg tgagggggac ctggggtctg gcggagggcg ccgaggtgac
     2161 gaccgaggcc aaccccgact ccgtcgacga ggccgcgctc gccgcgctcg cggacgccgg
     2221 cttcacccgc gtaagcctgg ggatgcagtc cgcggtcccg cacgtgctgc aaaccctgga
     2281 tagaacccac cggccagaga acgtgatgag
//
LOCUS       DASVKP010000113         3952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_781967, whole genome shotgun sequence.
ACCESSION   DASVKP010000113 DASVKP010000000
VERSION     DASVKP010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781967"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VF362_02370"
     CDS             complement(<1..237)
                     /locus_tag="VF362_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589802.1"
                     /translation="MSFFARLARFVTRHPVAVIVAWVIAAAAMFVFSEVGWGDYGNLQ
                     HRTTTGEPGVRGTDSAFALNATSASVPFEAGTRYS"
     gene            292..1629
                     /locus_tag="VF362_02375"
     CDS             292..1629
                     /locus_tag="VF362_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX7589803.1"
                     /translation="MDLFDAVRQDGSGLPSTSAASPLAVRMRPRTLDEVVGQSHLLER
                     GSPLRRLIEDKNAASSIMLWGPPGTGKTTLAYLVAGNRRFVELSAVSAGVKDVRAIID
                     DSKRRVATGEPETVVFIDEVHRFTKTQQDVLLPAVENRWITLVAATTENPSFSVVGPL
                     LSRSLLLTLRPLDEDAIRTLLARAVADERGLGGRVTLADDAREHIVRVAGADARKALT
                     LLENLAAAAGSGGEVTATLAEQAMDAALVAYDRQGDQHYDVISAFIKSVRGSDVDAAL
                     HYLARMIVAGEDPRFIARRLVVLAAEDIGMADPQGLVVAQAAADAVSFIGMPEGRIVL
                     AEATAYLATAPKSNRSYLAIDAAIADVKAGRIGTVPTHLRNATFPAARELGYGQGYAY
                     AHDAPHAVAPQQYLPDALADARYYEPSERGYERDVSARLAKIRDLLGRGEGGQ"
     gene            1728..2354
                     /gene="rpsD"
                     /locus_tag="VF362_02380"
     CDS             1728..2354
                     /gene="rpsD"
                     /locus_tag="VF362_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX7589804.1"
                     /translation="MTSVQRARRQVRLSRALGLALTPKAVKHFEKRPYPPGEHGRTAR
                     RKESDYAVRLREKQRLRAQYMVREKQMARVFEEAHKASGRTGDEMVELLEMRLDAVVL
                     RSGFARTILQARQTVLHRHILVDGHIVDRPSQRIMAGQTIQVKPKAATLDPYMAAAAG
                     AHRDVLPEVPDYLDVQLEKLTAKLIRRPKRAEVPITCDVQLVVEYYAR"
     gene            2406..>3952
                     /gene="alaS"
                     /locus_tag="VF362_02385"
     CDS             2406..>3952
                     /gene="alaS"
                     /locus_tag="VF362_02385"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX7589805.1"
                     /translation="MRTADIRQRWLDYFAGHGHEVVPSASLISPDPSLLFTVAGMVPF
                     IPYMLGQQQAPWARATSVQKCIRTLDIDEVGKTTRHGTFFQMNGNFSFGDYFKEGAIT
                     YAWDLITGSEGDGKYGFKPEDVWVTVFEDDDEAAALWEEIAGLPKERIQRRGMKDNFW
                     STGQPGPAGPCSEISIDRGREYGKDGGPVADEDRFLEIWNLVFMQYERGAGGGKDGFP
                     ILGELKQKNIDTGLGLERVAFLKQGVDNMYEIDEVVPVIHAAEALSGRVYGASNDDDV
                     RMRVVADHVRSSLMLMSDGVIPGNEGRGYVLRRLLRRSVRAMRLMGVEDVALPHLLPA
                     SKDAMKLSYPELEKQFAKISQIAYAEEDAFRRTLVSGTTILDTAVQRTKAEGGHLIDA
                     ADAFALHDTYGFPIDLTLEMASEHGLHVDEEGFRRLMQEQKERARADAKSKKGGHADI
                     KVYQGLAAEGLTEFTGYAELQTASEVRAIITDGKLAKRAAAGDIVEIVLPKTPFYAES
                     GGQEADAG"
BASE COUNT          664 a         1398 c         1304 g          586 t
ORIGIN      
        1 gctgtagcgc gtgcccgcct caaagggaac cgaagcgctc gtcgcgttga gagcgaacgc
       61 gctgtcggtg ccgcgcaccc ccggctcgcc cgtcgtcgtc cggtgttgca ggttcccgta
      121 gtcgccccag cccacctcgg aaaacacgaa catggcggcg gcggcgataa cccacgcgac
      181 aatgaccgcg accgggtggc gggtgacgaa acgggcgagg cgcgcgaaga agctcatggc
      241 caaaaaccta cacgcggcgc ccgcgtcgtg agtgcccaat agagtgaggt cgtggacctc
      301 ttcgacgcgg tgaggcagga cggatccggc ctcccgtcga cgtccgccgc gagccccctc
      361 gccgtgcgca tgcggccccg caccctcgac gaggttgtgg gccaatcgca cctcctcgaa
      421 cgcggtagcc ccctgcgacg actgatcgag gacaagaacg cggcctcgtc catcatgctg
      481 tggggccccc cgggaacggg gaagacgacg ctcgcctacc tcgtcgcggg caaccggcgc
      541 ttcgtcgaac tctccgcggt gagcgcgggg gtcaaggacg tccgcgcgat catcgacgac
      601 tcgaagcggc gcgtcgcgac gggcgagccg gagaccgtcg tgttcatcga cgaggtccat
      661 cgcttcacca agacccagca ggacgtgttg cttcctgcgg tggagaaccg gtggatcacc
      721 ctcgtcgccg cgacgaccga gaacccgtcc ttttccgtcg ttggcccgct cctttcccgc
      781 tccttgctcc tcacgcttcg ccccctggac gaggacgcga tccgcaccct cctcgcgagg
      841 gcggtcgccg acgagcgcgg gctcggcggc cgcgtcacgc tcgcggacga cgcacgggag
      901 cacatcgtgc gtgtggcggg cgccgacgcg cgcaaggccc tcacgttgct cgagaacctc
      961 gcggcagcgg cggggagcgg gggagaggtg acggcgacgc tcgccgaaca ggcgatggac
     1021 gccgcgctcg tcgcctacga ccggcagggc gaccagcact acgacgtcat cagcgccttc
     1081 atcaagtcgg tgcggggcag cgacgtcgat gccgcgctcc actacctggc ccgcatgatc
     1141 gtcgcggggg aggatccccg gttcatcgcc cgcaggctcg tcgtcctggc ggccgaggac
     1201 atcgggatgg ccgatccgca gggcctcgtc gtcgcgcagg ccgccgccga cgcggtgagt
     1261 ttcatcggca tgccggaggg cagaatcgtc ctggccgagg cgaccgcgta cctcgcgacc
     1321 gcgcccaagt ccaatcgctc ctatctcgcg atcgatgcgg ccatcgccga tgtcaaggcg
     1381 ggcaggatcg gcaccgtgcc cactcatctt cggaatgcca cgttccccgc cgcgcgggaa
     1441 ctgggctacg ggcagggcta cgcgtacgct cacgacgccc cccacgccgt cgcgcctcag
     1501 cagtacctcc cggacgcgct cgcggacgcc cgctactatg agccgagcga gcgcggatac
     1561 gagcgcgacg tctcggcgcg gcttgccaag atacgcgacc tgctcgggcg tggcgaaggc
     1621 ggccaatagt ccgctatcct tgtccgagcc ctcgcggcga gctggctgct cttgccgcgt
     1681 ccctgtagga cggcgcattc ccgcgccacg gtggttaaag gaagactgtg acttcagttc
     1741 agcgtgcgcg ccgccaggtg cgcctttccc gtgccctggg gcttgccctt acccctaagg
     1801 ccgtcaagca cttcgagaag cggccctacc cgcccggcga gcacggtcgt accgcgcgcc
     1861 gcaaggaatc cgactacgcc gtccgccttc gcgagaagca gcgcctgcgc gcccagtaca
     1921 tggtgcgcga gaagcagatg gcgcgcgtct tcgaagaggc ccacaaggcc tccggccgca
     1981 ccggcgacga gatggtcgag cttctggaga tgcgcctcga cgccgtcgtg cttcgttcgg
     2041 gcttcgcccg cacgatcctt caggcgcgcc agacggtcct ccaccgccac atcctcgtgg
     2101 acggccacat cgtcgaccgt ccgtcgcagc ggatcatggc gggccagact attcaggtca
     2161 agcccaaggc agcgaccctc gacccgtaca tggccgccgc agccggcgcc caccgcgacg
     2221 ttctccccga ggtccctgac taccttgacg ttcagctcga gaagttgacg gccaagctca
     2281 ttcggcgtcc caagcgcgcc gaggtgccga tcacctgcga cgtgcaactc gtcgtcgagt
     2341 actacgcccg atagcgtccc gcccctgacc cttcccccga ggagccgacc cgccgaggag
     2401 tgaccatgcg caccgctgac attcgccagc gttggctcga ttacttcgcc gggcacggcc
     2461 acgaggtcgt gccctcggcg tctctcatct cgcccgaccc gtcgctgttg ttcacggtgg
     2521 cggggatggt tccgttcatc ccgtacatgc tggggcagca gcaggccccg tgggcgcgcg
     2581 ccacctcggt ccagaagtgc atccgcaccc tggacatcga cgaggtcggc aagaccacgc
     2641 gccacgggac gttcttccag atgaacggca acttctcctt cggcgactac ttcaaagagg
     2701 gcgcgatcac ctacgcctgg gatctcatca ccggctccga gggggacggc aagtacggtt
     2761 tcaagcccga ggacgtctgg gtcaccgtgt tcgaggacga cgacgaggcg gccgcactct
     2821 gggaagagat cgctggcctg cccaaggagc gcattcagcg ccgcgggatg aaggacaatt
     2881 tctggtccac gggccagccg gggccggcgg gaccgtgctc cgagatctcg atcgaccgtg
     2941 gacgcgagta cggcaaggac ggcggcccgg tcgcggacga ggaccggttc ctcgagatct
     3001 ggaacctcgt cttcatgcag tacgagcggg gcgccggggg cggcaaggac gggttcccca
     3061 tcctgggcga gctcaagcag aagaacatcg atacagggct cgggctcgag cgcgtcgcgt
     3121 tcctcaaaca gggcgtcgac aacatgtacg agatcgacga ggtcgttccc gttatccacg
     3181 ccgccgaggc gctatccggc cgggtgtacg gcgcgagcaa cgacgacgac gtccgcatgc
     3241 gcgtcgtcgc cgaccacgtg cgctcgtcgc tcatgctgat gtccgacggc gtcatccccg
     3301 gcaacgaggg gcgcggctac gtcctgaggc gtctgcttcg ccgctccgtg cgcgcgatgc
     3361 gcctcatggg cgtcgaggac gtcgccctgc cgcacctcct ccccgcgtcg aaggacgcga
     3421 tgaagctttc ctacccggaa cttgagaagc agttcgccaa gatctcccag atcgcctatg
     3481 ccgaggagga cgcgttccgg cgcaccctcg tgagtggcac gaccatcctc gacacggccg
     3541 tccagcgaac gaaggcggag ggcgggcacc tcatcgacgc cgccgacgcc ttcgccctcc
     3601 acgacacgta tggcttcccc atcgacctca cgctggagat ggcctcggag cacggcctgc
     3661 acgtggatga ggagggattc cggcggctca tgcaggagca gaaggagcgc gcccgggccg
     3721 acgcgaagtc caagaagggc ggccacgccg acatcaaggt gtaccagggc ctcgccgccg
     3781 agggcctgac ggagttcacg ggctacgccg agctccagac ggcgagcgag gtccgcgcga
     3841 tcatcaccga cgggaagctc gccaaacgcg cagccgcggg agacatcgtc gagatcgtcc
     3901 tgccgaagac tccgttctac gccgagtcag gaggccagga ggccgatgcg gg
//
LOCUS       DASVKP010000114         6295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1520653, whole genome shotgun sequence.
ACCESSION   DASVKP010000114 DASVKP010000000
VERSION     DASVKP010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520653"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="VF362_02390"
     CDS             complement(<1..1368)
                     /locus_tag="VF362_02390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7589806.1"
                     /translation="MAIRPSVTRVTSGARRGFVVAVTLVAVILAVQLASSILSAVLAS
                     RDAQAATRQLFGVVGDVTIERVGRYSQAAEEAVSESVVSLERANPVTDVDAIASDLYG
                     RIHANPQVGAIKVGWSNGDYVELRREGAGYALHVVTVQPTRVTVVRSYDASFGLVSEG
                     PDPENATYDPRVRPWYTEGAAATGVAWTDPYLLFTTHTPAVSAVRAARVGNQVVAVVA
                     ADLLLDQLSTVLDGLPMGSGGEAFIFDADRNVVAAPGAYTSQIDGIGRNQGRVATAAD
                     LGATELPQGDAAQPGGAFGQDGGYVLLERDFSGATGPNWELHLRATRSGLAPALGAWE
                     GTVLWATLVSTLLVLIAVGLLVRVWGPLMGMRTRATTDALTGLPNRHELRSLGPRMVA
                     SAAADGAAICVALVDLDAFKEVNDSLGHKAGDDALVIAGRSLLDSTRARDLVARIGGD
                     EFVVIP"
     gene            complement(1378..2229)
                     /locus_tag="VF362_02395"
     CDS             complement(1378..2229)
                     /locus_tag="VF362_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX7589807.1"
                     /translation="MTGRPAGPDLAITLSNVGADRVKQVRALAGRSARLRAHRFVVEG
                     PQCVREAVRFAAGSVLDVYLTSAAHARSPEIAAEAAAAGLRVYGCSPEVAEAMSGDAQ
                     GAIAVVGLERAEGAEFPAAPRLVAILADARDPGNAGTVIRAADAAGADAVMLAGESVE
                     ATNPKVLRASAGSFFHLPVVEGRSLAEAVTVARAAGLRILATDGAGRVTLGDGAASAA
                     SLAVPTAWIFGNEARGLSPADLALADATVRIPMYGRAESLNLGAAATLCLFASAAAHR
                     TAADTAP"
     gene            complement(2235..2615)
                     /gene="rplT"
                     /locus_tag="VF362_02400"
     CDS             complement(2235..2615)
                     /gene="rplT"
                     /locus_tag="VF362_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX7589808.1"
                     /translation="MARVKRAVNAHKKRRTVLDRASGYRGQRSRLYRKAKEQVQHSLM
                     YAYRDRRARKGDFRKLWIQRINAAARANDLTYNRFIQGLKAANVDVDRRMLAEIAVSD
                     PAAFTLLVGIAKAALPKDVNAPAA"
     gene            complement(2643..2837)
                     /gene="rpmI"
                     /locus_tag="VF362_02405"
     CDS             complement(2643..2837)
                     /gene="rpmI"
                     /locus_tag="VF362_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX7589809.1"
                     /translation="MPKNKTHSGAKKRFTLTGTGKVKYGHAMNVHKFEEKSGSRKRRV
                     SVDGILAAPDAKRVKKLLGK"
     gene            complement(2936..3802)
                     /gene="infC"
                     /locus_tag="VF362_02410"
     CDS             complement(2936..3802)
                     /gene="infC"
                     /locus_tag="VF362_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX7589810.1"
                     /translation="MKGADINEPRINDKIRVPEVRLVGPEGEQVGIVRLEDALRLAQE
                     ADLDLVEVAPESRPPVAKLMDYGKFKYEAAVKAREARRNQSNTVVKEIRFRLKIDPHD
                     YETKKGHVVRFLKAGDKVKVTIMFRGREQSRPDAGRRLLQRLAEDVADLSIIEHAPVQ
                     EGRNMSLVLGPVRKKAEARDEARREREAKVAAETAEREAKKAPKTDAAKVAAAKAANP
                     KPSATKADAPKADAPKADAPVANVASPADDAPAAAKATVAKPAVAKPAVAKVPISPPT
                     TAKAAPKKTSGA"
     gene            3986..4312
                     /locus_tag="VF362_02415"
     CDS             3986..4312
                     /locus_tag="VF362_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEX7589811.1"
                     /translation="MADQQARDLADVSSVELITTVGVHLLSAAALRCGLAEDGETYQD
                     LDEARAIINALAGLVTAAAADLGDTHARALRDGLRSVQLAFREASPYPDPPGEGPGEK
                     YTGAVS"
     gene            4309..5043
                     /locus_tag="VF362_02420"
     CDS             4309..5043
                     /locus_tag="VF362_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589812.1"
                     /translation="MTNPPTPPTGGTQPTGAILPTGPVPRPGTDTNAIYRPDYGTGER
                     PRPGINPANEQTTVLTPVPERTAALNRIPLGERRRWQVLAAALGVAILVLGGLTAYLW
                     TTSDRWAARSASLEGQSYDLGQRLSTEQEYVVKQTEQIDILTQQLSTAQQRITDLADQ
                     SAQAGDDVAYAQQEITYLKELVSLGGSVSLALNRCTNEQKTLVGYLQNPSAYTAEGIA
                     QFKSDVDALCAAAQSANAELQKQLAQ"
     gene            5046..5759
                     /locus_tag="VF362_02425"
     CDS             5046..5759
                     /locus_tag="VF362_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX7589813.1"
                     /translation="MRKLLVATVALLLAGCSSLPSLPPPLPTDFVPSPSPTASDYGPL
                     LSPDGFDVAQRMTVRVRNIGCGFLATGTGFMIDEHTLVTNRHVIEDSSQLEVSTYDGH
                     AVDATAARSTTAADIAIIRVNETLPVAAHLAEKDPQTGDAITVIGYPGGGRLTTTSGI
                     VLGSVQDPLNGSVGTVLGTTAKVEPGSSGSPVLNAAGEVVGVIYAKNDVEQSFMIPVS
                     VLRQLLDQESLLIPQQNSC"
     gene            5867..>6295
                     /locus_tag="VF362_02430"
     CDS             5867..>6295
                     /locus_tag="VF362_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589814.1"
                     /translation="MTEEAAGQFSPGPPPLSMPPPLPESAVHGDFPPRPQSSGLEAPA
                     TMDAPAPRSGLLRRIFRPAVVWTLGALVVVAGNAWMYHVIDGWRAEDAALRHRATVAG
                     NSVGESNASLAGGAELLGLLKSQSDATSARAAKVEADGAAQ"
BASE COUNT          947 a         2246 c         2157 g          945 t
ORIGIN      
        1 cgggatgacg acgaattcgt cgccgccgat ccgtgcgaca aggtcacggg cccgcgtcga
       61 atcaaggagg gagcggccgg caatcacgag cgcgtcgtcc ccggccttgt ggcccaggga
      121 gtcgttgacc tccttgaacg cgtcgagatc gacgagcgcg acgcagatcg ccgcaccatc
      181 agccgcggcg gaggcaacca tgcgcggtcc caggcttcgg agttcgtggc ggttcggcag
      241 ccccgtgagc gcgtccgttg tcgctcgggt ccgcatgccc atgagtggcc cccacacgcg
      301 aaccaggagg ccgacggcga tgaggacgag cagcgtcgac acgagcgtcg cccacagcac
      361 cgtcccctcc caggcgccca gggcgggcgc gaggccggat cgggtggccc gaaggtgaag
      421 ttcccagttc ggccccgtcg cgcccgagaa gtcccgctcg aggaggacgt agccgccatc
      481 ctggccgaac gcaccccccg gctgggcggc gtcgccctgc ggcagttcgg tggcgcccag
      541 gtcggccgcg gtggcgacgc gaccctggtt ccggccgatg ccgtcgattt gggacgtgta
      601 ggcccccggc gcggccacga cgttgcggtc ggcgtcgaag atgaaggcct ctcccccgct
      661 ccccatcggg aggccgtcga ggaccgtgga gagttggtcg aggagcaggt ccgccgcgac
      721 caccgcgacg acctggttcc cgacgcgcgc cgcgcgcacc gcggagacgg cgggggtgtg
      781 cgtggtgaac agcagatatg gatccgtcca cgcgaccccg gttgcggcgg cgccctcggt
      841 gtaccagggc cggactcgag gatcgtaagt cgcgttctcg gggtcggggc cctcgctcac
      901 aagcccgaag ctcgcgtcgt acgagcgaac caccgtcacc cgcgtcggct gcacggtgac
      961 gacgtgaagg gcgtacccgg cgccctcgcg gcggagttcc acgtagtcgc cgttcgacca
     1021 ccccaccttg atcgccccca cctgcgggtt cgcgtggatc cgcccgtaga ggtcagacgc
     1081 gatggcgtcg acatcggtca cgggattggc gcgctcaagc gagacgacgg actcgctgac
     1141 cgcctcctcc gccgcctgcg agtaccgccc gacgcgctcg atcgtcacgt caccgacgac
     1201 gccgaagagt tgccgcgtgg cggcctgcgc gtcccgggag gccagaaccg ccgagaggat
     1261 cgaagacgcg agttgaacag cgaggatgac ggcgacgagc gtgacggcga cgacgaagcc
     1321 gcgtcgcgcg ccggaggtca cgcgggtaac gctgggacgg atcgccaccc cagtagccta
     1381 cggcgcggtg tccgcggcgg tgcggtgcgc cgccgcgctc gcgaagaggc agagcgtggc
     1441 cgcggccccg aggttgaggc tctccgcgcg gccgtacatc ggaatgcgga cggtcgcgtc
     1501 ggcgagcgcg aggtctgcgg gcgagaggcc ccgcgcctcg ttcccgaaga tccacgccgt
     1561 gggcaccgcg agggacgccg cgctcgcggc gccgtcgccg agcgtcaccc tccccgcgcc
     1621 gtccgtcgca aggatgcgaa gccccgcggc gcgcgcgacc gtgaccgcct cggcgagcga
     1681 gcggccctcg acgacgggaa ggtggaagaa gctaccagcc gaggcgcgca ggaccttcgg
     1741 gttcgtggcc tcgacgctct cccccgcgag catgacggcg tccgcgcccg cggcgtcggc
     1801 ggcgcgaatg acggtgccgg cgttgccggg gtcgcgcgcg tccgcgagga tcgccaccag
     1861 ccgcggtgcg gcggggaact ccgccccctc cgcgcgctca agcccgacga ccgcgattgc
     1921 gccctgcgcg tcgccgctca tcgcctcggc aacctcaggg ctgcacccgt agacccgaag
     1981 tcccgcggcg gccgcctccg cggcgatctc ggggctacgg gcatgagcgg cactcgtcag
     2041 gtacacgtcg agcaccgagc ccgcggcgaa gcggacggcc tcgcggacac actgcggccc
     2101 ctcgacgacg aaccgatgcg cccggagacg cgccgaacgc cccgcgaggg ccctgacctg
     2161 cttgacgcga tccgcgccca cattggacag cgtgattgcc aggtcggggc ccgcggggcg
     2221 tccggtcacg agactcaggc cgcgggggcg ttgacgtcct tcggcagcgc cgccttcgcg
     2281 atgccgacga gcagggtgaa cgcggcgggg tcgctgacgg cgatctcggc gagcatgcgc
     2341 cggtcgacat ccacgttggc ggccttgagg ccctggatga accggttgta ggtcaggtcg
     2401 ttcgcgcggg ccgcggcgtt gatcctttgg atccacagct tccggaagtc acccttgcgc
     2461 gcgcgacggt cgcggtacgc gtacatgagc gagtgctgga cctgctcctt ggccttgcgg
     2521 tacaggcgcg accgctgtcc ccggtagccg cttgcgcggt cgaggacagt ccggcgcttc
     2581 ttgtgggcgt tgaccgcccg cttgacgcgt gccacttcag tactccttgc gtggttgggg
     2641 ggctacttgc ccagaagctt cttgacgcgc ttggcgtcgg gagcggcgag gatgccgtcg
     2701 acggagacgc ggcgcttgcg cgaacccgac ttctcctcga acttgtgaac gttcatggcg
     2761 tggccgtact tcaccttgcc ggtgcccgtc agcgtgaatc gcttcttcgc acccgagtgg
     2821 gtcttgttct tcggcatggc gccgtctctc ctcgtgtctg gccccacccc gaccggggtg
     2881 aggcgtcttc ttgttcggac ccgcgtccct ggagggaccc gcgtccctct tcggactacg
     2941 cgcccgacgt cttcttcggg gccgccttcg cggtagttgg tggggaaatc gggaccttcg
     3001 ccacggcggg cttggccacg gcgggcttgg ccacggtggc cttggccgcc gcaggggcat
     3061 cgtcggcggg ggaagccaca ttggcgaccg gggcgtcagc cttcggggcg tcagccttcg
     3121 gggcgtcagc cttcgtcgca gagggcttcg ggttcgcggc ctttgcggca gcaaccttgg
     3181 ccgcgtcggt cttcggagcc ttcttggcct cgcgctccgc cgtctcggcg gcgaccttgg
     3241 cctcgcgctc gcggcgtgcc tcgtccctgg cctcggcctt cttgcggacg gggccgagaa
     3301 cgagcgacat gttgcggccc tcctggaccg gcgcgtgctc gatgatggac aagtcggcga
     3361 cgtcctcggc caggcgctgc aggaggcgac gaccggcgtc ggggcgcgac tgctcgcggc
     3421 cgcggaacat gatggtcacc ttgaccttgt cgcccgcctt gaggaagcgc acaacatggc
     3481 ccttcttggt ctcgtagtcg tgcggatcga tcttgaggcg gaagcggatc tccttgacga
     3541 ccgtgttcga ctgattcctg cgcgcttcgc gcgccttgac cgcggcctcg tacttgaact
     3601 tgccatagtc catgagtttg gcgacggggg gacgcgactc gggggcaacc tcgaccaagt
     3661 cgagatccgc ctcctgggca agcctcagcg cgtcctcgag ccgtacgatt ccgacctgtt
     3721 cgccctcggg gccaacgagt cggacttccg gcactcggat cttgtcgttg atacgcggtt
     3781 cgttgatgtc cgctcctttc acgggtgccg cccggcaaag gaagaggccc ctgccaaaac
     3841 gcacgcagag gccacaaaac cccgtcgggc gctaccgccc atcgggaccg attaccctgg
     3901 cccatgaatg gcccgagggt gggagtggac tccacttgcg cgcgacgaga attgccgtcg
     3961 ccggtcgata ggcaagaata gcaccatggc cgatcagcag gcgcgggacc tcgcggacgt
     4021 ctcgtccgtc gagctgatca cgaccgttgg ggtccatctg ctcagcgccg cagcactccg
     4081 ctgcggactc gccgaggatg gcgagacgta ccaggatctt gacgaggcgc gcgcgatcat
     4141 caacgcgctc gcgggcctgg tgacggcggc ggctgcggac ctcggcgaca cccacgcgcg
     4201 cgccctgcgc gacggcctcc ggagcgtaca gttggctttc cgggaggcgt cgccgtatcc
     4261 ggatccaccg ggcgaggggc ccggcgaaaa gtacacggga gcagtgtcgt gaccaacccc
     4321 cccaccccgc ctaccggagg aacccagccc acgggcgcga tcttgccgac cggcccagtc
     4381 ccgcgccccg gcacggacac caacgccatc taccggcccg actacggcac cggcgagcgg
     4441 ccccggccgg ggatcaaccc cgccaacgaa cagaccaccg tcctcacccc cgtccccgag
     4501 cgcacggccg ccctcaaccg catcccgctc ggcgagcgtc gtcgctggca ggtgctcgcc
     4561 gccgctctcg gcgtggccat cctggtgctc ggtggtctca cggcctatct ctggaccacg
     4621 agcgacaggt gggcggcgcg ctccgcatcc cttgagggtc agtcgtacga ccttgggcaa
     4681 cgcctgtcga cggagcagga gtacgtcgtc aagcagacgg agcagatcga catcctcacc
     4741 cagcaactct cgacggcgca gcagcgcatc accgacctgg cggaccagtc ggcccaggcg
     4801 ggagacgacg tcgcctacgc gcagcaggag atcacgtacc tcaaggagct cgtgtccctc
     4861 ggcggctcgg tctcgcttgc gcttaaccgc tgcacgaacg aacagaagac cctcgtcggg
     4921 tacctgcaga acccgagcgc ctataccgcc gagggcatcg cccagttcaa gtccgacgtc
     4981 gacgcgctgt gcgccgcggc ccaaagcgcg aacgcggaac tccagaagca gctggcgcag
     5041 tagccatgcg caaactgctc gtggcgaccg tcgccctgct ccttgccggg tgctccagcc
     5101 tcccgagcct ccccccgccc ctcccgaccg acttcgtgcc ctccccgtcc ccgacggcgt
     5161 cggactacgg ccccctgctc tcccccgacg ggttcgacgt tgcccaacgc atgaccgtgc
     5221 gggtccgaaa catcgggtgc ggattcctgg cgacgggcac gggcttcatg atcgacgagc
     5281 acaccctcgt gacgaaccgt cacgtgatcg aggactcaag ccaacttgag gtttcgacct
     5341 acgacggcca cgccgtcgac gcgaccgcag cgaggagcac caccgccgca gacatcgcca
     5401 tcatccgcgt caacgagacg cttccggtcg cagcgcacct tgccgagaag gaccctcaga
     5461 ccggagacgc gatcacggtg atcgggtatc cgggcggggg acggctcacg acgacctcgg
     5521 gaatcgtgct cgggagcgtc caggatcccc tcaacggctc ggtcgggacc gtcctcggaa
     5581 ccaccgccaa ggtggaaccg ggatcgtccg gctccccggt gctgaacgcc gccggggaag
     5641 tcgtgggcgt catctatgcg aagaacgacg tggagcagag cttcatgatc ccggtgtcgg
     5701 tgctccggca actgctcgac caggagtccc tcctgattcc tcagcaaaac tcctgctgac
     5761 gcgcgcggtc gccaagcgtg cgcgaggcgt ccgggcaggg tagtttggcg ggaacgccgt
     5821 cgttgttgcc gcacggctcg cgaggatccg atcgaacggt gcgcgcatga ccgaggaagc
     5881 cgcagggcag ttctcccccg ggcccccgcc actgtccatg cccccgccac tgcccgagtc
     5941 cgcggtgcac ggggatttcc ccccgcgccc gcaatcctcc ggccttgagg cacccgccac
     6001 catggatgcc ccggcaccaa ggagcggtct tctccggcgg atcttccgac ccgcggtcgt
     6061 gtggacgctc ggcgccctcg tcgtcgtcgc gggcaacgcg tggatgtatc acgtcatcga
     6121 tggatggcgc gccgaggatg cagcccttcg ccaccgtgcg accgtcgcgg ggaactcggt
     6181 cggggaatcc aatgcttcgc tcgcgggcgg ggcggaactg ctcgggcttc tgaagtccca
     6241 atcggacgca acgtcggccc gggcggccaa agtcgaggcg gacggcgccg cgcaa
//
LOCUS       DASVKP010000115         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1029437, whole genome shotgun sequence.
ACCESSION   DASVKP010000115 DASVKP010000000
VERSION     DASVKP010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029437"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="VF362_02435"
     CDS             <1..168
                     /locus_tag="VF362_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX7589815.1"
                     /translation="GISPHLKPAVRDVLTVAASLAARSQVGGTAPERVAEQGRAARLR
                     VKFFRPWTGGV"
     gene            complement(192..653)
                     /locus_tag="VF362_02440"
     CDS             complement(192..653)
                     /locus_tag="VF362_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589816.1"
                     /translation="MNEKKVLGSIRKYTLWAVLGSAALLLVLEILKAWDVVKTTSGGG
                     MANLADAALSALMGGSLDALGGLNFSAPTVNAWGHIEDTLGIIVGIGALVLLVLLVSS
                     RTKVGKIKDGKGKAGKVAAAKVAVVTVTAPAAKAPVAKAPAAKAAAAEPKK"
     gene            951..1094
                     /locus_tag="VF362_02445"
     CDS             951..1094
                     /locus_tag="VF362_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589817.1"
                     /translation="MVLKGHMLDVLLAAVATLGLAITLSVQNAAAMDVPPPMAAQNHV
                     LAP"
     gene            1203..1796
                     /locus_tag="VF362_02450"
     CDS             1203..1796
                     /locus_tag="VF362_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7589818.1"
                     /translation="MSSQRGADLPSIEGLRLLVLAAPARLGRTRLVLVDGPAGSGKTS
                     LAGRLAAALGCDILHGDDMYEGWGGLPTLWETLGEQVLEPLARGEAARFRRWDWARGE
                     RAEWIEVEAVDGRGADALVVEGVGVAGRRARPYASLGIYVEAPRDVRLARGVERDGEA
                     LRDEWLRWEAAEAPVLLAEGTREASDLIIDGTIPVPD"
     gene            complement(1839..>3133)
                     /locus_tag="VF362_02455"
     CDS             complement(1839..>3133)
                     /locus_tag="VF362_02455"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010205055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX7589819.1"
                     /translation="HSVGAPLAPGQADVTWPCNDTKYISHFPETREIWSYGSAYGGNA
                     ILAKKSFALRIASVMARDEGWLAEHMLLLKVTNPRGRVFHVTAAFPSACGKTNFAMLR
                     PSIPDWKVETLGDDIAWIAPGADGRLRAINPEAGFFGVAPGTGEGTNPTAIETLWGNV
                     IFTNVALTDDGDVWWEGLTDEPPAHLIDWRGNDWTPESETTAAHPNSRFAVAAQQCPS
                     IADTWDDPAGVPLDAIIFGGRRASNVPLVAQSYDWAHGVFMGATLASERTAAAEGTQG
                     ELRRDPFAMLPFCGYHMADYWAHWLAMGNKLEQSGLKQPPIFMVNWFLKGEDGRFLWP
                     GYGENSRALAWILDRVDNAATAEAGPTGLVPASGALDAAAAGVSAEDSRRLFAVDHGA
                     WSSEADDIASYFATFADRLPPALWDELARLRGRLEPPA"
BASE COUNT          467 a         1036 c         1136 g          494 t
ORIGIN      
        1 gggatttcgc cgcacctcaa gcctgccgtg cgggacgtgc tgaccgtggc ggcgtcgctc
       61 gcggcgcggt cccaggttgg cggaaccgcg ccggagcgcg tcgcggagca ggggagggcg
      121 gccaggctgc gggtcaagtt cttccggccc tggacgggcg gagtttagaa ccgcgagccg
      181 taccccccgc cctacttctt gggctcggcg gctgcggcct tcgcagcagg ggccttcgct
      241 actggagcct tggcggcggg cgccgtaacg gtgacgaccg cgaccttcgc cgcggccacc
      301 ttgccagcct tgcccttgcc atccttgatc ttgccgacct tcgtccggct gctcaccagg
      361 agcacgagca ggacgagcgc gccgatcccg acgatgatgc cgagcgtgtc ctcgatgtga
      421 ccccacgcgt tcacggtcgg cgcggagaag ttgagtccgc cgagcgcgtc gagcgaaccg
      481 cccatgaggg cgctgagcgc ggcgtcggca agattcgcca tgccgccgcc gctcgtcgtc
      541 ttgacgacgt cccacgcctt gaggatctca aggacgagga gcagcgccgc cgaaccgagc
      601 acggcccaca gggtgtactt cctgatgctg ccaagcacct tcttttcgtt cattctgcag
      661 tctccttagc ggggcgaata tgcccgactt cgaccctagc gcgaaggcgt cgttcgcgcc
      721 tgcccctgtg gccctcggga cgccctgggc gagaaatggt tttcggaggg tggcgaatgt
      781 ggcgtcccac atagccggtt tgtggtgcct gccacagtgt gaggcacccc accgaaaccg
      841 ccgcgcggag ggcgcgggga gtcgtaccgt ggaggaagaa ggacaacgaa gaagttgtcc
      901 gacactctcg gccacaagcc gcagacagag gaagagggaa ggtgagtgtg atggtactca
      961 agggccacat gcttgacgta ctactcgccg ccgtagccac gctcgggctc gcgatcacgc
     1021 tgagcgtgca gaacgcagcc gccatggatg tcccgccgcc gatggcggcg cagaatcacg
     1081 tcctcgcacc ctagccaccc agggctaggc gcggcgccat cggctccgag cctgtcgacg
     1141 gcgccgccta cttttggaat gggcgccgcc cactggaaat gggcgccgcc tattcttggc
     1201 agatgagttc acagcggggc gccgatctcc cgtccattga ggggctccgc ttgctcgtct
     1261 tggcggcccc cgcccggctc ggccggaccc gcctcgttct cgtcgacggg cctgcgggct
     1321 cgggtaagac cagcctcgcc ggacgcctcg ccgccgccct ggggtgcgac atccttcacg
     1381 gcgacgacat gtacgaggga tggggcgggc tcccgacgct gtgggagacg ctgggggagc
     1441 aggttctgga gccgctcgcc cggggcgagg cggcccgatt ccgccggtgg gattgggcgc
     1501 gcggggagcg cgcggagtgg atcgaggtgg aggcggtcga cgggcggggg gccgacgccc
     1561 tagtggtgga gggcgtcggg gtcgccgggc ggcgagcgcg gccgtacgcg agcttgggta
     1621 tctacgtcga ggcgccgcgg gacgtgcggc tcgcgcgggg cgtcgagagg gatggggagg
     1681 cgctacggga cgagtggctc cggtgggagg ccgccgaggc gccagtactg cttgccgagg
     1741 gcacgcgcga ggcttccgac ctcatcatcg acgggaccat tccggttccc gactaggcgt
     1801 ctccggggcg cgcgacgcgt ggaggcgcgc gcgggccgct aggcgggcgg ctcgagccgg
     1861 ccgcgaagcc gcgcgagctc gtcccacagc gcgggtggga gtctgtccgc gaacgtcgcg
     1921 aagtacgagg cgatatcgtc cgcctcggac gaccaggcgc cgtggtcgac cgcgaagagt
     1981 ctccgcgagt cttcggcact cactccggct gcggcggcgt cgagcgcgcc cgaggcagga
     2041 accaggccgg tcggccccgc ctccgcggtc gcggcgttat ccacccggtc gaggatccag
     2101 gcgagcgcgc gcgagttctc gccgtagccg ggccagagga accgaccgtc ctcgcccttg
     2161 aggaaccaat tgaccatgaa gatcggcggc tgcttcaacc ccgactgctc gagtttgttg
     2221 cccatcgcga gccaatgcgc ccagtagtcg gccatgtggt acccgcagaa cggcagcatc
     2281 gcgaacgggt cgcggcgcaa ctcgccctgg gtgccctcgg ccgcggcggt gcgctccgag
     2341 gcgagagtgg cgcccatgaa gactccgtgc gcccagtcgt acgattgcgc gacgagcggc
     2401 acattcgaag cgcggcgacc accaaagatg atggcgtcga gcggcacccc tgcggggtcg
     2461 tcccacgtgt ccgcgatgga cggacactgc tgcgcggcaa cggcgaatcg cgagttgggg
     2521 tgcgcggcgg tcgtttcact ctcgggcgtc cagtcgttgc ccctccaatc gatgaggtgc
     2581 gccggcggct cgtcggtcag cccctcccac cagacatcgc cgtcgtccgt gagcgcgacg
     2641 ttggtgaaga tgacgtttcc ccagagcgtc tcgatcgccg tgggattcgt gccctcgcct
     2701 gtgcccggcg cgactccgaa gaatcccgcc tcaggattga tggcgcgtag ccgcccgtcg
     2761 gcgcccggtg cgatccaggc gatgtcgtcg ccgagcgtct ccaccttcca gtcggggatg
     2821 gacgggcgga gcatcgcgaa attggtcttg ccgcacgccg aggggaacgc tgcggtgacg
     2881 tggaagaccc tgccgcgcgg gttcgtgacc ttgaggagca gcatgtgttc ggccaaccat
     2941 ccctcgtcgc gcgccatcac ggacgcgatg cgcagcgcga aggacttctt cgcgaggatc
     3001 gcgttgccgc cgtatgcgct gccgtaggac cagatttccc gcgtttcggg gaagtgggag
     3061 atgtacttgg tgtcgttgca cggccacgtg acgtcggcct gaccaggggc gagcggtgcg
     3121 ccgacagagt gaa
//
LOCUS       DASVKP010000116         3982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_618903, whole genome shotgun sequence.
ACCESSION   DASVKP010000116 DASVKP010000000
VERSION     DASVKP010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618903"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VF362_02460"
     CDS             complement(<1..470)
                     /locus_tag="VF362_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589820.1"
                     /translation="MRGLARTILALVVVSATLALSACGGGPSTDEVAAWAAFSAYRQG
                     QHHDTPVQGPRSVKEYVLGATGAYKAKYSGADSILTGKFTGAETRLDPATGTTDVWAT
                     FSIHAPVMGQVTGTAVIDIGDAPPGVDVDKAVRGAGNAVVFLVDDGGTWHLSDGL"
     gene            complement(499..3036)
                     /gene="glgP"
                     /locus_tag="VF362_02465"
     CDS             complement(499..3036)
                     /gene="glgP"
                     /locus_tag="VF362_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX7589821.1"
                     /translation="MKPRERVTVRTVLPDTLGGLEILARNLRWSWHAPTRELFASLDS
                     EAWARARGNPVVFLHSLSGSRLASIAADPALIARIDGAVANLAEYLAAPSWYGTEVTG
                     GPEVIAYFSPEYGIAAALPQYSGGLGILAGDHLKSASDLGVPIIAVGLLYGAGYFRQG
                     LDATGRQTESYPHLDPAAMPLALLREDDGTPTIVAIELPGDRMLHAQVWRADVGRVRM
                     LLLDSNTHLNTPDLRGLTERLYGGREEERLLQELLLGVGGVRALRAFSRLTGAPAPGV
                     FHMNEGHAGFLGVERIRELVAQGWTFAQALEAVRGGTVFTTHTPVPAGIDRFPRLMIE
                     HHFSLASWGVGVEDVLALGAEANGGANPDVFNMACMGLRLGGRANGVSLLHGKVSREM
                     FGSLWPGFDESEVPIGSITNGVHGPTWVDPRLAALAAARIPTDDAWVSDAIPDGELWA
                     LKREMRSTLVEEARIRLRASGLQRAASAASLRWVEMALDPDILTIGFARRVPTYKRLT
                     LMLRDPDRLKALLLHPERPIQILVAGKAHPHDEGGKALIQELVAFADHADVRHRIVFL
                     PNYDIAMAEKLYPGCDVWLNNPLRPLEACGTSGMKAALNGALNLSVLDGWWDEWHDES
                     NGWAIPTADGVVDEARRDDIEAAALYDLIEREVAPLYYHRDANGIPVGWLKRVRHTLA
                     TLGPKVQATRMVRDYTLLEYLPAAASATALALDDGAPAKELAAWKAKVRAAWPTLAIA
                     HVYSEGLDAPVLVGREATVTAEVALNGLVTDDVRVQLLSGPLDSRGEAPQYSVADMTA
                     EGDVGGRARFVAHILLDESGPWGYTVRVVPSHPLLASVADLGLVAQA"
     gene            3137..>3982
                     /locus_tag="VF362_02470"
     CDS             3137..>3982
                     /locus_tag="VF362_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEX7589822.1"
                     /translation="MSSAPRPSVGRIPVLGVGPSLEEGRWPARAVVGEAVPVWATIMR
                     EGHDAVAATLVVTAPDGATTSAPMPCEDFGMSLYRASTIPSTPGDYVFAVEAWSDPVT
                     TWRTVADVKLHAGQDIQLVLAEGVAVLERAAEQPGRTAADAKLLYGAIAALRDSRLDP
                     EARVAAAMSARVRALLERAPLREWVTRSAEYPLRVDRERALVSAWYEMFPRSEGARYD
                     APTKAWVSGTFATAARRLPVIARMGFDIVYLTPIHPIGTSFRKGRNNSLDAAPGDPGS
                     PYAIGS"
BASE COUNT          598 a         1388 c         1420 g          576 t
ORIGIN      
        1 agtccgtcgg acaggtgcca ggttccccca tcgtcgacga ggaacacgac ggcgttgccc
       61 gcgccccgca cggccttgtc gacgtccacg cccgggggcg cgtcgccgat gtcgatgacc
      121 gccgtgcccg tcacctgccc catgacgggc gcgtgaatcg agaaggtcgc ccagacgtcg
      181 gtcgtcccgg tcgcgggatc caggcgcgtc tccgctccgg tgaacttccc cgtcaggatc
      241 gaatcggctc cggagtactt cgccttgtac gcccccgtcg cgccaagaac atactccttg
      301 acgctccgcg gcccctgcac cggggtgtcg tggtgttgcc cctggcgata ggcggagaac
      361 gcggcccacg cggcgacctc gtcggtgctc ggccctccgc cgcacgcgga aagcgcgaga
      421 gtcgccgaga cgacgacgag ggcgaggatg gtacgcgcga gacctctcat gccgccaggg
      481 taccgcccgg caggcctcct acgcctgtgc aaccagcccg aggtccgcaa ccgaggcgag
      541 caggggatgc gagggaacca cccgcaccgt gtagccccat ggccccgatt cgtcgaggag
      601 gatgtgggcc acgaagcgag cccgtccgcc gacgtctccc tcggccgtca tgtcggcgac
      661 gctgtactgt ggcgcctcgc cccgcgagtc gagtggacca gacaggagct gaaccctcac
      721 gtcgtctgtg acgaggccgt tcagcgcgac ctccgcggtt accgtggctt cgcgcccgac
      781 gagaacggga gcgtcgagcc cctccgagta gacgtgcgcg atcgcgaggg tgggccacgc
      841 ggccctgact ttggccttcc acgccgcaag ttcctttgct ggcgcaccat cgtccaaggc
      901 gagcgccgtt gccgacgccg ctgcgggaag gtactcgagc aacgtgtagt cgcgcaccat
      961 gcgggtcgcc tggaccttgg ggccgagcgt cgcgagcgtg tggcgcaccc gcttcagcca
     1021 cccgacgggg atgccgttcg cgtcgcggtg gtagtacagg ggcgctacct cgcgttcgat
     1081 gaggtcatac agcgcggccg cctcgatgtc atcgcggcgc gcctcgtcca ccacgccgtc
     1141 ggcggtggga atcgcccagc cgttggactc gtcgtgccac tcgtcccacc agccgtcgag
     1201 cacggagagg ttgagggcac cattgagggc ggccttcatg cccgacgttc cgcacgcctc
     1261 gagcggccgg agcgggttgt tgagccagac gtcgcaaccg gggtagagct tctccgccat
     1321 cgcgatgtcg tagttcggca ggaagacgat gcggtggcga acgtccgcgt ggtccgcgaa
     1381 ggcgacgagc tcctggatga gggccttgcc gccctcgtcg tgggggtgcg ccttgcccgc
     1441 gacgaggatc tggatgggcc gctcggggtg aagcaggagc gccttgagtc ggtcgggatc
     1501 gcgcagcatc agggtgaggc gcttgtacgt cgggacgcgt cgcgcgaagc cgatggtgag
     1561 gatgtcaggg tccagggcca tctcgaccca cctcagggac gccgcggagg cggcccgctg
     1621 gagcccggat gcccgaagcc ggatgcgcgc ctcttcgacg agggtcgagc gcatctcgcg
     1681 cttgagtgcc cacaactcgc cgtcggggat cgcgtcgctc acccaggcgt cgtccgtcgg
     1741 aatccgcgcc gccgcgagcg cggcgaggcg ggggtccacc cacgtgggcc cgtggacccc
     1801 gttcgtgatg gagccgatcg gcacctccga ctcgtcgaag cccggccaca ggctcccgaa
     1861 catctcgcgg gaaaccttgc cgtggagcag ggagacgccg ttggcccggc cgccgaggcg
     1921 gagccccata cacgccatgt tgaagacgtc ggggttcgcc ccgccgttcg cctccgctcc
     1981 gagcgcgagg acgtcctcca cgcccactcc ccacgacgcg agggagaagt ggtgctcgat
     2041 catcagccgc gggaagcgat cgatccccgc gggcacgggg gtgtgggtgg tgaagaccgt
     2101 gccgccgcgc accgcctcga gggcctgcgc gaaggtccac ccctgcgcga cgagttcgcg
     2161 gattcgctcg acgccgagga agccagcgtg gccctcgttc atgtggaaca ccccgggcgc
     2221 cggggcgccg gtaagccggg agaacgcgcg taaggcgcgg acgccgccga ctccgaggag
     2281 caactcctgg agtagccgct cctcctcgcg accgccgtag agacgctcgg tgaggccacg
     2341 gagatcgggg gtgttgaggt gggtgttgga gtcgagtagg agcatccgca cgcggccgac
     2401 gtcggccctc cacacctggg cgtgaagcat ccggtcgccg gggagttcga tcgcgacgat
     2461 cgtcggcgtc ccgtcgtcct cgcggagcag cgcgagcggc attgccgcgg gatcgaggtg
     2521 ggggtaggac tccgtctggc ggcccgtggc gtcgaggccc tggcggaagt atccggcacc
     2581 gtacaggagg ccaacggcga tgatcggcac gccgaggtcg gaggcgctct tcaggtggtc
     2641 gcccgccaga atcccgaggc cccccgagta ctgcggaagc gccgccgcga ttccgtactc
     2701 cggcgagaag tacgcgatca cctcggggcc gccggtgacc tccgtgccgt accacgacgg
     2761 cgccgcgagg tactcggcga ggttcgcgac cgcgccgtca atccgcgcga tgagggcggg
     2821 gtcggccgcg atcgacgcca gcctgctccc gctgagggag tgcaggaaga cgacggggtt
     2881 gccccgcgcg cgcgcccacg cctcagaatc gaggctcgca aacagttcgc gagtgggggc
     2941 gtgccacgac caacggaggt tgcgggcgag gatctccagc ccgccaaggg tgtcgggaag
     3001 gacggttctg acggtgacgc gttcgcgagg tttcaccttg gaaggctacc cgcgtcgtgt
     3061 atcccgcaag taacgcgcgt gtgagccacg caacgacggt tacccatcag acgccgcggg
     3121 ccggtagttt cgaaccatgt cctccgcccc tcgcccctcc gttgggcgaa tcccggtcct
     3181 cggcgtcggg ccttccctcg aggaggggcg gtggccggcc agggccgtcg tcggcgaggc
     3241 cgtgcccgta tgggcgacga tcatgcgcga gggccacgac gccgtcgcag caacgctcgt
     3301 cgtgacggcc cctgacggcg cgacgacgtc ggcgccgatg ccgtgcgagg acttcggcat
     3361 gagcctgtat cgcgcctcga cgatcccgtc gacgccgggc gactacgttt tcgcggtcga
     3421 ggcgtggtcg gacccggtga cgacgtggcg caccgtcgcc gacgtcaaac ttcacgcggg
     3481 ccaagacatc cagctcgtcc tggccgaggg cgtcgcggtg ctcgaacgtg ccgccgaaca
     3541 gccggggcgc acagccgccg acgcgaagct cctctatggc gcgatcgcgg ccctccgcga
     3601 cagtcggctc gaccccgagg cgcgggttgc cgcggccatg agcgcaaggg tgcgcgccct
     3661 actggaacgg gcgccgctca gggagtgggt cacgcgctcc gcggagtacc cgctgcgcgt
     3721 cgaccgcgag cgcgcactcg tgagcgcctg gtacgagatg ttcccgcgct ccgagggcgc
     3781 gaggtacgac gcccccacca aggcctgggt ctcgggcacg ttcgcgacgg cggcgaggcg
     3841 gcttcccgtg atcgcgcgca tgggcttcga catcgtctac ctcaccccga tccaccccat
     3901 cggcacctcc ttcaggaagg gccgcaacaa ttcgctcgac gccgccccgg gagacccggg
     3961 aagcccctac gccatcggct cg
//
LOCUS       DASVKP010000117         2879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_865362, whole genome shotgun sequence.
ACCESSION   DASVKP010000117 DASVKP010000000
VERSION     DASVKP010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865362"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1829)
                     /locus_tag="VF362_02475"
     CDS             complement(<1..1829)
                     /locus_tag="VF362_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HEX7589823.1"
                     /translation="MRQIAVSGGEVKVLDVPAPVVRPGGVLVRTHFSVISVGTESASM
                     GGGGRESLLMKAIRNPALVRKVIDRVSSHGIQSTAELVRSKLSNDQATGYSCAGIVLE
                     VGAGVTGFSAGDRVACAGAGYANHAEINFVPVNLIAKVPEGLALDEAAFATLGAIALQ
                     GVRRCAPTLGERVVVLGLGLLGQLTVQLLRANGCVVLGVDVRRDRVEKARSLGMTDGF
                     AVAERDFVAGVLERTEGRGADAVIVTAAGGDSALLNRSFEACRRKGRVILVGDVPIRI
                     QRDRIYKKELDFLISTSYGPGRYDAAYEEKGHDYPFGYVRWTEGRNMGEVLRLMASGA
                     LSVRPLIDAVHDVEKAPDAYASLAVEPRSVGVVLRYGSSDSGTGSLGRTHLSTRATQR
                     RPGSLRVGVIGYGSYFRSALLPILRAQPGVELASVCARNGLSVRGAVEKDGFLRGTTD
                     YREMLADPNVDVVYVATRHDQHYPIACAAVEAGKAVFVEKPMTMRAAEGRDLVERVAR
                     RNTLLTVGFNRRFSPHVVRLREALAPIAGPKTMLYRVNAGPLPPDHWLRDPVEGGGRL
                     LGEGVHFFDLLRHLAGSEIRDVMAAPGASARDEATVSLSFADGS"
     gene            complement(1861..>2879)
                     /locus_tag="VF362_02480"
     CDS             complement(1861..>2879)
                     /locus_tag="VF362_02480"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX7589824.1"
                     /translation="WVEERGRRFREIRSSAHVNSEVVGVADLDQTGRWENTPDYGNAW
                     TPSNVEKDWQPYRNGRWLWQDPWGWTWVSEESWGWAPYHHGRWTAYPAGWFWVPVGPT
                     AKYVSYSPALVAFLGGGADGSAAQGFRGGGDVGWFPLALQDPFYPWWIPRLDTHLTKV
                     TEFVYRNRKNITVVPSKAFASGELVRANLVRDAAVLRDALASPVLRVPLPLLPTQDSL
                     HMAATSKAGRNEEPSAAILTQPIVARATPPPGPPTFGAKLEAIRQNRGAPVPPSVAIR
                     IRQADGRKAVAGMAVVPVVRPDGRMTLAPRHPDASAPRPQPIKPAAGLPPTRVPSPGR
                     VQAG"
BASE COUNT          501 a          990 c          970 g          418 t
ORIGIN      
        1 gaaccatccg cgaacgacag gctcaccgtg gcctcgtcgc gcgccgacgc tcccggagcg
       61 gccatcacgt cccggatctc gcttcccgcg aggtgtcgca ggaggtcgaa gaagtgcacg
      121 ccctcgccaa ggagacgtcc tcctccttcg acggggtcgc gaagccagtg gtccggcggg
      181 agaggcccgg cgttcacgcg atagagcatc gtcttcgggc ccgcgatcgg cgccagcgcc
      241 tcccgcaggc gcacgacgtg cggagagaac cgccggttga atccgacggt gagaagagtg
      301 ttgcggcgcg cgacacgctc gacgaggtcg cggccttcgg ccgccctcat cgtcatcggc
      361 ttttcgacga acacggcctt acccgcctcg acggccgcac acgcgatcgg gtaatgctga
      421 tcgtgacgcg tcgcgacgta tacgacgtcg acgttcggat cggcgagcat ctcgcggtag
      481 tcggtcgtgc cgcggaggaa gccgtccttc tcgaccgccc cgcgcacgga cagtccgttg
      541 cgcgcgcaca cagacgcaag ctcaacgccc ggctgggcgc ggaggatcgg aagaagcgcg
      601 gaccggaagt agcttccgta tccgatgacc cccacgcgaa gggaaccggg ccgacgctgg
      661 gttgcccggg tcgacaggtg cgtgcgcccg agggaaccgg tcccggaatc tgaagatccg
      721 taccggagga cgacgcccac agaccggggc tccacggcga gagaggcgta ggcgtccggc
      781 gccttctcca cgtcgtgcac ggcatcgatg aggggccgca cggacagggc accggacgcc
      841 atgaggcgca gcacctcgcc catgttgcgc ccctcggtcc agcgcacgta tccaaatggg
      901 tagtcgtggc ccttctcttc gtatgcggcg tcgtaacggc ccggcccgta ggacgtcgag
      961 atgaggaaat ccagctcctt cttgtagatc cggtcgcgct ggatcctgat gggaacgtca
     1021 ccgacgagga tgacgcggcc ctttcggcgg cacgcttcga acgagcggtt caagagggcc
     1081 gaatcgccgc ccgccgcggt cacgatcacc gcgtccgcgc cacgcccctc ggtccgttcg
     1141 aggactccag ccacgaaatc gcgctcggca acggcgaatc cgtccgtcat cccgagtgaa
     1201 cgcgccttct cgacacggtc gcgccggacg tcgacgccga ggacgacgca gccgttcgcg
     1261 cgcaggagct gcaccgtgag ctgccccagg aggccgagcc cgagaacgac gacgcgttcg
     1321 ccgagcgtgg gggcacaacg cctcacgccc tgcagcgcga tcgcgccaag cgtcgcgaag
     1381 gccgcctcgt cgagcgcaag gccctcgggg accttcgcga tgagattcac gggaacgaaa
     1441 ttgatctcgg catgattcgc gtatcccgcg ccggcacatg cgacccggtc tcccgcggag
     1501 aagccggtca cccccgcgcc gacttcgagg acgatgcccg cgcacgaata gccggtggcc
     1561 tgatcgttcg agagcttcga acgcacgagc tccgccgtgc tctggattcc gtgcgaggac
     1621 acgcggtcga ttaccttccg gacgagggcc ggattgcgga tcgccttcat gaggaggctc
     1681 tcgcgccccc cgccgcccat ggacgcggac tcggtgccga cgctgatgac cgagaaatgc
     1741 gtccgaacga gcacaccgcc cggacgtacg acgggcgccg ggacgtcgag gaccttgact
     1801 tccccgcccg agaccgcaat ttgtcgcacg agaaatccta acaccagcga agagtggggg
     1861 tcagcccgcc tggactcgcc ctggactggg gactcgagtc ggtggcagac ctgccgcggg
     1921 cttgatcggc tgggggcgtg gcgcagaggc gtccggatgg cgcggcgcca gcgtcatgcg
     1981 gccgtcgggc ctgacgacgg ggacgacggc cataccggcg acggctttac gcccatcggc
     2041 ctgtcggatt ctgatcgcga cggacggagg aaccggagcg cctcgattct gccggatggc
     2101 ttcgagcttt gcgccaaacg tcggcggtcc cggaggaggt gtggcacgag ccacgatcgg
     2161 ctgcgtcagg atcgccgccg agggctcctc gttgcggccc gctttcgacg tggccgccat
     2221 gtgaagcgaa tcctgcgtcg ggagaagcgg cagagggacc cgcaagacgg gggaagccag
     2281 ggcatcccga agaaccgccg catcgcgcac gaggttggct cgtaccagct cgccggacgc
     2341 gaacgccttc gatgggacga cggtgatgtt ctttcgattc cgatagacga attccgtgac
     2401 tttcgtcaga tgcgtgtcga ggcgcgggat ccaccacgga tagaagggat cctggagcgc
     2461 cagtggaaac cacccgacat cgcccccacc tcggaagccc tgcgccgcgg acccgtcggc
     2521 gcctccgcca aggaaggcga cgagcgcggg cgaatacgac acgtacttgg ccgtcgggcc
     2581 caccggcacc cagaaccagc cggcaggata ggccgtccat cgcccgtggt gatagggagc
     2641 ccatccccac gactcctccg acacccacgt ccagccccag gggtcctgcc agagccagcg
     2701 gccgttgcgg tacggctgcc agtccttctc gacgttcgaa ggcgtccatg cgttgccgta
     2761 gtcgggagtg ttctcccagc gcccggtctg gtcgagatcc gcgacaccga cgacctcgga
     2821 gttgacgtga gccgaggacc ggatctcgcg gaatcggcgc ccccgttcct cgacccagc
//
LOCUS       DASVKP010000118         2624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1360289, whole genome shotgun sequence.
ACCESSION   DASVKP010000118 DASVKP010000000
VERSION     DASVKP010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360289"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VF362_02485"
     CDS             complement(<1..925)
                     /locus_tag="VF362_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589825.1"
                     /translation="MRIFRGFLAVVCFVLGLVSLAVWAASTFMVRAVEDGTMVTAVAQ
                     KVVQQPWVTNLITEQAQSVVKAKLAANGIDLASVGLQGTLDDAIAAAIDSDAFRAMLR
                     DSVDGARADLARQLTDPAAEGQPLVLQIDVGATINRTIAEVPLVGSFMPTVAFDPVPV
                     PVADAREFDQLRRDYRWIHRGATWAGWVALLFIAAGIALTPRKRWLLPLALLFAGLGG
                     GALWAALRLFSVDRIVGALPADGLGGHLRDAIVQYAPQHTIERFGNRVLLASAACLIA
                     ALVAFIIVKVASRPARRRADAVEAHHPHRQHE"
     gene            complement(928..>2624)
                     /locus_tag="VF362_02490"
     CDS             complement(928..>2624)
                     /locus_tag="VF362_02490"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEX7589826.1"
                     /translation="HRVQMARMRRTHLLPDSEEGLRALARASGFPTAEALLEAWHAIR
                     RDVRSMHLEMFYRPILPVVARLSAGEASLDTDAARERLAAIGFADPESAQRHLAALTE
                     GVSRKAAIQRQLLPAMIGWFAEGPDPDAGLLAFRTLSEQLKDASWYLKMLRDSGTAAE
                     RLARLLSTSAYVTEALLASAEDVTWLDKDEDLVPRDEARLVHEADAILARSQDVDQCI
                     TALRGLRRRELARTAAADVLGLLDTLEAATTITHVADVVLGGALRIAMSEVGGGKAPF
                     DMAIIAMGRYGGMEMSYPSDADVMFVYEPHEGPDPAEADRIAQHVATEVRRLLQTANP
                     QPALDVDVDLRPEGKNGPLARSLASTAEYYGRWSEPWEAQALLRARPAAGDPGVRARF
                     VEAIDAIRFPSELGPEALKQMRTLKARMESERLPRGIDPRRHVKLGPGGLADVEWTIQ
                     LLQLLHAHEHPELRTTLTLPAIEAAVTAGVLGRDDAEVLAVAWRLATRLRAAMALRGY
                     ARQRTNVIPSDARELKVLAGILGDGWTGAQLDEYYARAARRARVVMERVFYGWEKE"
BASE COUNT          399 a          922 c          924 g          379 t
ORIGIN      
        1 cttcgtgctg cctgtgcgga tggtgggcct cgaccgcgtc ggcccgccgc cttgcgggac
       61 gcgaagcgac cttgacgatg atgaaggcga ccagcgccgc gatcaggcag gcggcggacg
      121 cgagcaggac gcgattgccg aagcgctcga tggtgtgctg cggcgcgtat tgcacgatgg
      181 cgtcgcgtag gtggccgccg agcccgtccg cgggtagcgc gccgacgatg cgatcgacgc
      241 tgaacagacg cagcgccgcc cagagggcgc ccccgccaag ccccgcaaac agcagcgcga
      301 gcggaaggag ccagcgcttc cgtggcgtca gcgcgatccc tgccgcgatg aagaggagcg
      361 ctacccaacc ggcccacgtc gcgccgcggt gtatccaccg gtagtcgcgg cggagttgat
      421 cgaactccct cgcgtccgcg acgggcacgg gaaccggatc gaacgcgacc gttggcatga
      481 aggagccgac cagcggcacc tccgcgatcg tccggttgat ggtggcgccg acgtcgattt
      541 gcaggacgag gggctggccc tccgcggcgg ggtccgtcaa ctgccgggca agatccgccc
      601 gcgcgccatc gacggagtcc ctcagcatgg cgcggaacgc gtcggaatcg atcgccgcgg
      661 cgatcgcgtc gtcgagcgta ccttggagcc ccaccgaggc gaggtcgatg ccgttcgccg
      721 cgagtttggc cttgacgacg gactgggctt gctccgtgat cagattcgtc acccagggtt
      781 gctgcacgac cttctgcgcg accgctgtga ccatcgtgcc gtcctcaacg gccctgacca
      841 tgaacgtcga cgcggcccac accgcgagcg acaccagccc gagcacgaag cagaccacgg
      901 cgagaaagcc tcgaaagatc ctcatcgcta ctccttctcc caaccgtaga agacccgctc
      961 catgaccacg cgcgccctcc gcgccgccct ggcgtagtac tcgtccagct gcgctcccgt
     1021 ccaaccgtcg ccaaggattc ccgcgagcac cttgagttcg cgcgcgtcgc tcgggatgac
     1081 gttggtccgc tggcgcgcgt agccccgcag cgccatggcc gcccgaagcc tcgttgcgag
     1141 ccgccacgcc acggccagaa cctcggcgtc gtcgcggccg agaaccccag ccgtgacggc
     1201 ggcctcgatc gcgggaagcg tcagggtggt gcgcaactcg ggatgctcgt gggcgtggag
     1261 gagttggagc aactggatgg tccactcgac atcggcgagt ccccctggcc ccagtttgac
     1321 gtggcgtcgt gggtcgatcc ctcgcgggag gcgttcggac tccatcctcg ccttgagcgt
     1381 ccgcatctgc ttcagagcct cgggccccag ctccgagggg aagcggatgg cgtcgatcgc
     1441 ctcgacgaac ctggcgcgca cgcccggatc gcccgccgct ggacgcgcgc ggagcagcgc
     1501 ctgagcctcc cacggctccg accaccgccc gtagtactcg gcggtcgagg cgagagagcg
     1561 cgcgagcggc ccattcttgc cctccggtcg aagatcgacg tcgacgtcga gggcgggttg
     1621 ggggttcgcc gtctgcagga gccgccggac ctcggtcgcg acgtgttggg cgatccggtc
     1681 ggcctccgcg gggtccggcc cctcgtgggg ctcgtagacg aacatgacgt cggcgtcgga
     1741 cgggtagctc atctccatgc cgccgtagcg acccatagcg atgatcgcca tgtcgaaggg
     1801 agccttccct cccccgacct cgctcatcgc gatgcggagc gccccgccca acacgacgtc
     1861 cgcgacatgg gtgatcgtcg tcgcggcctc gagggtgtcg aggagcccga ggacgtccgc
     1921 ggccgccgtg cgcgcaagtt cgcgcctgcg gaggcccctc agggccgtga tgcactggtc
     1981 cacgtcctgc gagcgggcca ggatcgcgtc ggcctcgtgg acgaggcgcg cctcgtcccg
     2041 gggcacgagg tcctcgtcct tgtctagcca cgtgacgtcc tccgccgagg cgagcaacgc
     2101 ctccgtgacg taggccgagg tcgacaacag gcgcgcgagc cgctccgccg ccgttccgga
     2161 gtcgcggagc atcttgaggt accaggacgc gtccttcagt tgctcggaga gggtccgaaa
     2221 cgcgaggagg ccagcgtcgg gatcgggccc ctccgcgaac cacccgatca tcgcgggaag
     2281 gagctggcgc tggatcgccg ccttgcggga gacgccttcg gtgagggccg cgaggtggcg
     2341 ctgggccgac tcggggtcgg cgaatccgat ggcggcgagc cgctcgcggg ccgcatcggt
     2401 atcgagcgag gcctcgcccg cgctcagccg cgccacgacg gggaggatgg gacggtagaa
     2461 catctccagg tgcatggatc ggacgtcgcg acgaatcgcg tgccacgcct cgaggagcgc
     2521 ctccgccgtg gggaagcccg aggctcgcgc gagggcccga agcccctcct cggagtccgg
     2581 gagcagatgg gtcctgcgca tccgggccat ctggaccctg tgct
//
LOCUS       DASVKP010000119         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_866236, whole genome shotgun sequence.
ACCESSION   DASVKP010000119 DASVKP010000000
VERSION     DASVKP010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866236"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..101
                     /locus_tag="VF362_02495"
     CDS             <1..101
                     /locus_tag="VF362_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HEX7589827.1"
                     /translation="RVTSKSRDDLQAAMALLKGADVEAALQFVNYR"
     gene            complement(137..1000)
                     /gene="htpX"
                     /locus_tag="VF362_02500"
     CDS             complement(137..1000)
                     /gene="htpX"
                     /locus_tag="VF362_02500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX7589828.1"
                     /translation="MSRRYFNGVKTFGLLIGMWVLMLGLGAVIGKGQYIWLFGAGALL
                     MNVYAYWNSGKMSIRAMQARPVTEVEQPAMYRIVRDLSREANQPMPQLFVSPTDAPNA
                     FASGRNPRNAVVCCTEGILQILNERELKGVLGHELMHVYNRDILVGSVAAASAGMITM
                     IAQMLFFFGGGRRNSNPLVLIAMLVLAPIAATLIRLAISRTREYDADEDGSKLTGDPM
                     ALASALKKIEAGVAARPLAQTSELENVAHFMIANPFKGAGLARMFATHPPIEDRVSRL
                     EDMAANRGFIR"
     gene            complement(1097..2293)
                     /locus_tag="VF362_02505"
                     /pseudo
     CDS             complement(1097..2293)
                     /locus_tag="VF362_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837792.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
BASE COUNT          357 a          760 c          798 g          378 t
ORIGIN      
        1 ttcgggtgac gagcaagagc cgcgacgacc ttcaggccgc gatggctctg ctcaagggcg
       61 ccgacgtcga ggccgcgctc cagttcgtca actaccgcta ggacgccccc gggcgatagc
      121 tggccagaag ccgcgcctag cggatgaagc cgcggttcgc ggccatgtcc tcgagccgcg
      181 acacgcggtc ttcgatcggg gggtgggtcg cgaacatccg tgcgaggcct gcgcccttga
      241 acgggttcgc gatcatgaag tgcgcgacgt tctccaactc gctcgtttgg gcgagcggcc
      301 gcgccgcgac cccggcctcg atcttcttca gcgcggacgc gagcgccatc gggtcgcccg
      361 tgagcttcga gccgtcctcg tccgcgtcgt actcgcgggt gcgggagatc gcgaggcgaa
      421 tgagggtggc ggcgatgggt gcgaggacga gcatcgcgat gaggacgagg gggttggagt
      481 tgcgccggcc gcccccgaag aagaagagca tctgggcgat catcgtgatc atcccggcgg
      541 aggcggccgc gacggagccg acgaggatgt cgcggttgta gacgtgcatg agttcgtggc
      601 cgagcacgcc cttgagctcg cgctcgttga ggatctggag gatcccctcc gtgcagcaga
      661 cgacggcgtt gcgcgggttg cggccggacg cgaaggcgtt gggggcgtcg gtgggcgaga
      721 cgaagagttg gggcatgggc tggttcgcct cgcgcgagag atcgcggacg atgcggtaca
      781 tcgccggctg ctccacctcg gtaaccggcc gcgcctgcat ggcgcggatc gacatcttgc
      841 ccgagttcca gtacgcgtag acattcatga ggagcgcgcc cgcgccaaag agccagatgt
      901 actggccttt gccgatgacg gccccgagcc cgagcatgag aacccacatg ccgatgagca
      961 gaccgaacgt cttcactccg ttgaaatacc tgcgactcac gatttcctcc cccacaagaa
     1021 cggccggtcg ggccgccgtg ttccagtatc gcgcggctct cgcctcaggg cgaacagggc
     1081 tcgcggacct cgggcgctag ccgcatccca gtgccacgtc cgtcatctcc tcgcgcggaa
     1141 cgaccttgat tcgttcgcgt ccctgggatt cccctagggc ctgctcgtgg gcgtccagca
     1201 cttcccagtc ctcccactcc acgacgcgca cgccgcggtc cttcagtagc gtgaggaggt
     1261 tgtctgggct cagggacacc ggctcggcgc ggctcgcggc gatcaggctc tcggcgtcgt
     1321 cgacgacgtg cccgatggtc tcctgggcgt cggacttggt gtggccgatc aggccgaccg
     1381 ggccccgctt gatccagcct gtcgtgtaga agccggcgac cgcgaccccg gcgtcgtcaa
     1441 cgacgcgacc ctcgacgttg cgaatcacgc cgcgcgacgc gtcgaacggc acgccaggaa
     1501 tctccgtgcc ccagtacccc accgcgcgat agacggcctg gcacgggtac tcaaggaatt
     1561 cccccgtgcc cttggccgtg ccgtcgccct ggagcgccat gcgttccatg cgcacgcccg
     1621 cgacgcgacc cgcgccgtcg tccagcacgg ccaccggctg ctggagcaag tggaggtgga
     1681 tgcggcgcga tgcccccgtg ggttcgcgga gtctccagtc catcagcgtc ttgaccgtgt
     1741 gcttggtctg atggtggctt tcgatcgccg cccgcgatgc ctcgtcgaag tcgtagtcct
     1801 cggggtagac gaccacgtcg acgtcggaca cctccccaag ttcgcggagc tccagcgggg
     1861 tgaacttcac ctgggcgggg ccgcgccgcc cgaacaggtg cacgtcggtc acggcggact
     1921 tctccagccc ctgaagaacg ttcgcgggga cgtccgtctc gagcagatcc ttcgggtgct
     1981 tggcgaggat gcgggcgacg tcgagcgcga cgttgccgac gccgaggatg gcgacctcgc
     2041 gggcctcgag cggccacgag cggggcacgt cgggatggcc gtcgtaccag gagacgaagt
     2101 ccgcggcgcc gaaggagccg tccatgtcga tcccgggtat gtcgaggcgg gcatcctcga
     2161 aggaccccgt cgcaaagacg accgcatcgt agtgatcgcg caggtcctag aggctgaggt
     2221 ccgtcccgac gttcacgttg gccaggagcc gaatgtctcc gcgttccagc acgttggtga
     2281 gcgcggtgat gat
//
LOCUS       DASVKP010000120         2682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_866336, whole genome shotgun sequence.
ACCESSION   DASVKP010000120 DASVKP010000000
VERSION     DASVKP010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866336"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /gene="sucD"
                     /locus_tag="VF362_02510"
     CDS             complement(<1..810)
                     /gene="sucD"
                     /locus_tag="VF362_02510"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX7589829.1"
                     /translation="MTIFLNASSRVIVQGMTGSEGQKHTARMLASGTQIVGGVNPRKA
                     GESVSFDLAGETRTIPVFGTVADAMAATSADVSVVFVPPPFAKAAVMEAIDAAMPLVV
                     IITEGVPVADTAVFTAHALERGTRTLGPNCPGVISPGQSNAGITPATITGPGPVGLVS
                     KSGTLTYQMMHELRDLGFTTCVGIGGDPVIGTTHIDCLAAFEADPDTKAIVMIGEIGG
                     DAEERAAAYIKEHVTKPVVGYVAGFTAPEGKTMGHAGAIVSGSSGTAQAKKE"
     gene            complement(820..1980)
                     /gene="sucC"
                     /locus_tag="VF362_02515"
     CDS             complement(820..1980)
                     /gene="sucC"
                     /locus_tag="VF362_02515"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX7589830.1"
                     /translation="MDLYEYQARDLFERHGVPVLEGIVATTPADARAAAAKIGGTVVV
                     KAQVKVGGRGKAGGVKLAHNPDEAEAAAAAILGMDIKGHTVRRVMIAAGARISEEYYF
                     SLVLDRATRRYLAMCSVEGGVEIEVLAVERPESLAKVDVDPRVGIDAAKAAEIVGAAG
                     FAPDVAGAVAEVLVKLGEVYVGEDATLVEVNPLVLTAEGAVIALDGKVTLDDNARFRH
                     PDHEEFVDIDATDPLERLAGEKGLNYVKLDGEVGIIGNGAGLVMSTLDVVALAGKDLG
                     VKPANFLDIGGGASAQVMADGLEIILGDAQVKSVFVNVFGGITSCVEVARGIVAALEI
                     LGDRATKPIVVRLDGNSVVEGREILSAAKHPLVTVVDTMDAAAAKAAELAAA"
     gene            complement(2121..2357)
                     /locus_tag="VF362_02520"
     CDS             complement(2121..2357)
                     /locus_tag="VF362_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589831.1"
                     /translation="MSTTIIMYSVFLIMFVGALLPKRRQWVAMVGAILVAGLGIFADT
                     PAFPWEAKVAAWLVVVALLVWRLLRDRKRRVDLT"
BASE COUNT          399 a          947 c          905 g          431 t
ORIGIN      
        1 ctccttcttg gcctgcgccg tgccggacga accggagacg atcgcgccgg catgtcccat
       61 cgtcttgccc tccggggcgg tgaaacccgc cacgtagccg acgacgggct tggtcacgtg
      121 ctccttgata tacgcggcag cgcgctcctc ggcgtcgccg ccgatctcgc cgatcatgac
      181 gatcgccttg gtgtccgggt ccgcctcgaa cgccgcgagg caatcgatgt gcgtggtgcc
      241 gatcacgggg tcgccgccga tgccgacgca cgtggtgaac ccgaggtccc gtagttcgtg
      301 catcatctgg taggtcaggg ttccggactt ggagacgagc ccgacggggc cgggcccggt
      361 gatggtcgcg ggcgtgatgc ccgcgttgga ctggccgggc gagatgacgc cggggcagtt
      421 cgggccgagg gtgcgtgtgc ccctctcgag cgcgtgcgcc gtgaagacgg ccgtgtcggc
      481 cacggggacg ccctcagtga tgatgacgac gagcggcatc gccgcgtcga tcgcctccat
      541 gacggcggct ttcgcgaacg gcggcggcac gaagacgacg gagacgtcgg cgctcgtggc
      601 ggccatcgcg tcggcgaccg tcccgaagac cggaatggtg cgcgtctctc ccgcgaggtc
      661 gaagctcacc gactcgcccg ccttgcgcgg gttgaccccg ccgacgatct gggtgccgga
      721 cgcgagcatg cgggccgtgt gcttctgacc ctcggagccc gtcatgccct ggacgatgac
      781 ccgcgaggag gcgttcagga agatggtcat gggtctctcc taggctgccg cgagttcggc
      841 ggcctttgcg gccgcggcgt ccatggtgtc caccacggtg acgagggggt gcttcgcagc
      901 gctcaggatc tcgcgaccct cgaccacgct gttgccgtcg agccgcacga cgatcggctt
      961 ggtcgcgcgg tctcctagga tctcgagcgc cgcgacgatg ccgcgcgcca cctccacgca
     1021 cgaggtgatc cctccaaaga cgttgacgaa gacgctcttc acctgagcgt cgccaaggat
     1081 gatctcgagg ccgtccgcca tgacctgcgc cgacgcgccc ccgccgatgt cgaggaagtt
     1141 cgcgggcttg acgcccaggt ccttgcccgc gagcgcgacg acgtcgaggg tggacatcac
     1201 gagccccgcg ccgttgccga tgatgccgac ctcgccgtcg agcttgacgt agttgagacc
     1261 cttctcgccc gcgaggcgct cgagggggtc cgtcgcgtcg atgtccacga actcctcgtg
     1321 atcggggtgg cggaagcgcg cgttgtcgtc gagcgtcacc ttcccgtcga gcgcgatgac
     1381 cgcgccctcc gcggtgagca caagcgggtt gacctccacc agcgtcgcgt cctcgcccac
     1441 gtagacctct ccgagcttga cgagcacctc ggcgaccgcg ccggcgacgt cgggcgcgaa
     1501 gccagcggcc ccgacgatct cggccgcctt cgccgcgtcg atgcccaccc gcggatccac
     1561 gtccaccttc gcgagggact cgggccgctc gaccgcgagc acctcgatct cgacgccgcc
     1621 ctcgacggag cacatcgcga ggtagcggcg cgtcgcccgg tcgagcacga gcgagaagta
     1681 gtactcctcg gagatcctgg cgccggcggc gatcatgacg cggcggacgg tatggccctt
     1741 gatgtccatg ccgaggatcg cggcggccgc ggcctccgcc tcgtccggat tgtgcgcgag
     1801 cttgacgccg cccgccttgc cgcgcccgcc caccttcacc tgggccttga cgacgacggt
     1861 gccgccgatc ttcgcggccg cggcgcgggc gtcggccggg gtcgtggcga cgatgccctc
     1921 gagcaccggg acgccgtggc gctcgaacaa gtcgcgcgcc tggtactcgt acaggtccat
     1981 gagggtcctt ctcgcgtgtc gggacgcccg tgcaagcgcc gcgcttccgg gttccagagt
     2041 atccgccgga tctccccgcg acgtaaccgc gacgaacccg ccaccggagg ggcgcgccct
     2101 ggaagagcgc cccggccgtc ctaggtgagg tcgactcgcc gcttgcgatc tcggagcaat
     2161 cgccacacaa gtagggcgac aaccaccagc cacgccgcga ccttcgcctc ccatgggaaa
     2221 gcgggggtat ccgcgaagat tcccaagccg gccaccaaga tggcgccaac catcgccacc
     2281 cactgtcgcc tcttgggtaa taaagccccg acgaacatga tgaggaatac cgagtacatg
     2341 atgatcgtgg tgctcatggc gtttctcccg agcgacggtt ttaggtgtag cgagtgaccg
     2401 ccgtgaacca tccgagcaga gggtttgcag ccgactatcg gtagccgtag cactttccct
     2461 gtgcagagta ccccgccgcc ttgttcgcgc ggtcgaacct cccccctccg gggaaaccct
     2521 gagatcgcct tcctcgtcgg ggccaccggg aagccccttg catcgcctcg caggcttgcg
     2581 cgcctgcggg cgatggccga gtggtcccgt ctgcggcgtc ggaacgagtc ccgcggccca
     2641 ccccatgggc gattgggacc tcggcaacac cagggtgcag at
//
LOCUS       DASVKP010000121         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_207020, whole genome shotgun sequence.
ACCESSION   DASVKP010000121 DASVKP010000000
VERSION     DASVKP010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207020"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VF362_02525"
     CDS             <1..460
                     /locus_tag="VF362_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589832.1"
                     /translation="LEDARSLFWIQIPMTATLIAITFIGWKVLDVHLWVVAAGAGMAI
                     SYAVSTALRMVGLRKRLGGLEGRRVLSTHLRAIAAVLPAAGLGWLLLRVIPDSSGMSR
                     KADVAASAGWCVVIGSAMALVYGVGLVILRVDEWRSVIAGPLRRLGFRVP"
     gene            512..1750
                     /locus_tag="VF362_02530"
     CDS             512..1750
                     /locus_tag="VF362_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589833.1"
                     /translation="MTIAHRFALAAPGGWQIPGATAWDARDTRLDVPVTVLVADSGDP
                     ESILAVLTRARGVRDPRLARIVDVGIADVPAGGEGAEGDDAATERRAYVAVANPPSPT
                     ALAILESRILPASLARLLIRGAADALDAARAGGLDHGALSPGAIGVTSRGRVIVAGAG
                     LLSAFGGDARGATAADSRALATLFARAVLGLGDTADEDAREQDGAATLPDDLTKTERR
                     LVKHALRGQFPSTVDELLAALGAADGATLSAIRTQLRKCAPVLVPEVVELEPEGLERA
                     ETREGAAYGAEAPPAGATREEIEEWELEHLLEEEEAEEIPTLAEAILDLLHRRFPRSI
                     RITRYLEAAHARALRGPKVNGTAWTLVALLLLLTILVFIAVDVYQRPFVPTFDLHNQP
                     AHSYPSFTFGPSPTAVTPAP"
     gene            1892..>2136
                     /locus_tag="VF362_02535"
     CDS             1892..>2136
                     /locus_tag="VF362_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7589834.1"
                     /translation="MSDTRSLIIVGSGPAGYTAAIYAARAGLSPLVVAGSVTAGGALM
                     NTTEVENFPGFVTGIQGPELMETLQAQAEKFGAEVLF"
BASE COUNT          313 a          795 c          711 g          317 t
ORIGIN      
        1 gctcgaggac gcccggtcgc tgttctggat ccagataccc atgaccgcga cgctcatcgc
       61 aatcaccttc atcggctgga aggtcctcga cgtccacctg tgggtcgtcg cagccggggc
      121 aggcatggcg atctcctacg ccgtctcgac ggcactccgg atggtggggc tcaggaagcg
      181 cttgggcggc ctcgagggcc gccgcgtact gtcgacgcac ctccgcgcga tcgccgccgt
      241 cctgcccgcc gccggcctgg gatggctcct cctgcgcgtc atccccgact cgagcggaat
      301 gtcgcggaag gccgacgtgg cggcctcggc cgggtggtgc gtcgtcatcg gaagtgcgat
      361 ggcgctcgtc tacggcgtgg gcctcgtgat ccttcgcgtc gacgaatggc gaagcgtcat
      421 cgcgggccca cttcgcaggc tcgggttccg cgtaccatag cggtcaacaa cattcaggaa
      481 tagtaggggg agcgccagcg tgctttctgg catgacgatc gcccaccggt tcgcgctcgc
      541 cgcgcccggt gggtggcaga ttcccggcgc cacggcgtgg gacgcgcgcg acactcggct
      601 cgacgtgccg gtcacggttc tcgtcgcgga ctccggcgac cccgaatcca tcctcgccgt
      661 cctcacccga gcccgagggg ttagggatcc gcgcctcgcc cgcatcgtcg acgtcggcat
      721 cgccgatgtc cccgccgggg gagagggcgc cgagggcgat gacgctgcga ccgaacggcg
      781 cgcctacgtt gcggtcgcga atccgccgtc gcccactgcc ctggcgatcc tcgagtcgcg
      841 tatccttccc gcttccctcg cgcggctgct gatccgtggc gccgcggacg cactcgacgc
      901 cgctcgggcc gggggcctcg accacggggc cctctctccc ggcgcgatcg gcgtcacgag
      961 tcgcgggcgc gtcatcgtcg ccggggcggg cctgctctct gccttcgggg gggatgcgcg
     1021 gggggccacg gccgcggatt cccgcgcgct cgccaccctc ttcgcacgcg cggtcctggg
     1081 cctcggtgac actgcagacg aggacgcgag ggaacaagac ggggcggcca cccttcccga
     1141 cgacctcacg aagaccgaac gccggctcgt caagcacgcc ctccggggcc aattcccgtc
     1201 cacggtcgac gaactgctcg cggcgctcgg cgcggccgac ggcgcgacgc tctcggccat
     1261 ccgcacccag ctccgcaagt gtgccccggt gctggtgccc gaggtggtcg agctcgagcc
     1321 cgagggcctc gagcgcgcgg agacacgcga gggcgccgcc tacggcgcgg aggctccgcc
     1381 agcgggggcg acaagggagg aaatcgagga gtgggaactc gagcacctcc tcgaggagga
     1441 ggaagccgag gagataccga ccctcgcgga agccatcctc gaccttctcc atcgacgatt
     1501 cccccgctcg atccgcatta cccgctacct cgaggcggcg cacgcccggg cactccgcgg
     1561 gccgaaggtc aacggcaccg cgtggacgct cgtcgcactt ctcctcctgc tgaccatcct
     1621 ggtgttcatc gcagtcgacg tataccagcg gcccttcgtc ccgaccttcg acctgcacaa
     1681 ccagccggcc cactcctacc cgagcttcac cttcggtccc tcccccaccg cggtcacgcc
     1741 ggcgccgtag cccagtacac ctagcgccgc cgacgccggg gaacagaagg ccactaacat
     1801 ctgttaggga cgtgtgcgac gtcaccagcg ccccgggccc ccggcatatc ggcacttcag
     1861 aacgcagtga ccagaacgcg aggactgcag cgtgagcgac accagaagcc tcatcatcgt
     1921 gggatccggc cccgccgggt acacggcggc catctacgcc gcgcgggcgg gcctctcccc
     1981 gctcgtcgtg gcgggctccg tcaccgctgg cggcgcgctc atgaacacga ccgaggtgga
     2041 gaacttcccg ggtttcgtca cgggcatcca gggccccgaa ctcatggaga cgctccaggc
     2101 gcaggccgag aagttcggcg ccgaggtcct cttcga
//
LOCUS       DASVKP010000122         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_703371, whole genome shotgun sequence.
ACCESSION   DASVKP010000122 DASVKP010000000
VERSION     DASVKP010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703371"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VF362_02540"
     CDS             complement(<1..487)
                     /locus_tag="VF362_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7589835.1"
                     /translation="MTSVYDVAVAGSSGFLGSAIAAGMEARGATVARYTLENPMLQGG
                     VVDDGAARARTLVWAASRVNPVLAAERPDLVRAETADLADALRALAALPSPPRVVFLS
                     SGGTVYGPPDRAPFSESCATHPVNAYGSLKRGLERELLDSGLDVVAARISNAYGPGQI
                     PA"
     gene            520..1707
                     /locus_tag="VF362_02545"
     CDS             520..1707
                     /locus_tag="VF362_02545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7589836.1"
                     /translation="MGKPRLLVLASTYPARPGDGTPSFVRDLALGMAEAFDIEVLVPA
                     VPGGARHESDGALTVARFRYFWKRWEDLADGAILENVRARRSRLLQVPFLLVAEYVAV
                     RRAVRRRRPDAIHAHWIIPQGLVARLATRGIPTVVTTLGGDLYALRSGAARRLKAGVV
                     RHASAVTVMNDEMRAMVEALGANPAALSVMPMGFDPSSLATGMPRPPRDTVSVLFVGR
                     LVEKKGLAVLLAALRERPPAASWRLVVVGDGPLRADLEMVAASMPVSFVGQLGRTDLS
                     NAYIDADIAIFPSVRAATGDQDGLPVALLEAMGSGCAVIASDLPGLTEAVERGRSGVL
                     VPPGDSDALADALGLLADDPDRRRRLGERAAARARDFDVSTVARGYRDLLLRVLSDTT
                     PRR"
     gene            complement(1704..>2330)
                     /locus_tag="VF362_02550"
     CDS             complement(1704..>2330)
                     /locus_tag="VF362_02550"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7589837.1"
                     /translation="YGDLELDDPAKFTPDTPYNPSSPYSATKAGSDLLVRAWVRSFGV
                     EATISNCSNNYGPLQHVEKFIPRQITNILDGVRPKLYGAGLNVRDWIHVDDHNSAVWT
                     IIDDGKVGETYLIGANGELDNKTVIEMILAGMGEPADAYDHVSDRPGHDMRYAIDASK
                     LRTELGWEPRFRDFAAGLADTIAWYRVNEAWWRAQKAAAEAKYAKLGR"
BASE COUNT          320 a          824 c          797 g          389 t
ORIGIN      
        1 gcgccggaat ctggcccggt ccgtacgcat tcgagattct cgccgcgacc acgtcgagtc
       61 cactgtcgag gagctcccgc tcgagtcctc gcttgagcga gccgtacgcg ttgacggggt
      121 gcgtggcgca cgactccgaa aacggcgctc ggtccggcgg gccgtagacg gtgcccccgg
      181 aggaaaggaa cacgactcgg ggcgggctgg gtagtgcggc gagcgcacga agggcgtcgg
      241 cgaggtcggc cgtctcggca cggacgagat cgggccgctc ggccgcgagc accgggttga
      301 cacgggaggc cgcccagaca agcgtccgcg cgcgcgccgc gccatcgtcc acgacgccgc
      361 cctggagcat cggattctcc agcgtgtagc gcgccacggt ggctccccgg gcctccatgc
      421 ctgcggcaat cgcggagcca aggaagccag aggagcccgc gacggccacg tcatagactg
      481 aagtcacgca ccatcctccg cacctttgga cctcatccta tgggcaagcc ccgattgctc
      541 gtccttgcct caacgtatcc ggcaagaccc ggcgacggga ccccctcctt cgtgcgggac
      601 ctcgccctgg gcatggcgga ggccttcgac atcgaggtgc tggtacccgc cgttcccggc
      661 ggcgcgaggc atgagagcga cggcgcgctt accgtcgcgc gcttccgcta cttctggaag
      721 cgttgggaag acctcgcgga cggggcgatc ctcgagaacg tccgcgcgcg ccgctcgcgc
      781 ctcctccagg tcccgttcct cctcgtcgcc gagtacgtgg ccgtgcggcg cgcggtccgt
      841 cgacgtcgac ccgacgccat ccacgctcac tggatcattc cgcagggact cgtcgcgcgc
      901 ctcgcgacgc ggggtatccc caccgtggtg acgaccctgg gcggagatct ctacgccctt
      961 cgttccggag ccgcacgacg actgaaggcg ggggtggtcc ggcacgcctc cgccgtcacg
     1021 gtgatgaacg acgagatgcg ggcgatggtg gaggctctcg gagccaaccc cgccgccctg
     1081 tcggtgatgc ccatgggctt cgacccgtca tcgctcgcca cggggatgcc gcgacccccc
     1141 cgcgacaccg tttccgtcct cttcgtcgga aggctcgtgg aaaagaaggg gctggccgtc
     1201 cttcttgcgg cgcttcgcga acgccctccc gcggcatcct ggcggctcgt cgtcgtcggc
     1261 gacgggccgc tgcgggccga ccttgagatg gtggcggcct ccatgccggt ctcgttcgtc
     1321 ggccagcttg gacgcacgga tctatcgaac gcctacatcg atgccgacat cgccatcttc
     1381 ccctccgtcc gagccgcgac cggcgaccag gatgggctcc ccgtggcgct gcttgaggcc
     1441 atgggttccg gctgcgcggt gatcgcgagc gaccttcccg gcctgaccga ggccgtcgag
     1501 cgaggccgta gcggcgttct cgtcccgccg ggggactccg acgcgctcgc cgacgccctt
     1561 ggcctccttg ccgacgatcc cgatcggcgc cgtcggctcg gggagcgggc cgccgcccgg
     1621 gcgcgcgact tcgacgtttc gaccgtcgcg aggggctacc gggatctgct ccttcgggtc
     1681 ctcagcgaca cgactccgcg ccgctagcga cccagcttcg cgtacttcgc ctccgcggcg
     1741 gccttctgcg cgcgccacca cgcttcgttc acgcggtacc aggcgatcgt gtcggcgagg
     1801 cccgcggcga agtcgcggaa ccgcggctcc caacccagtt ccgtgcggag cttgctcgcg
     1861 tcgatcgcgt agcgcatgtc gtggccgggg cgatccgaga cgtggtcgta cgcgtcggct
     1921 ggctcgccca tccccgcgag gatcatctcg atcacggtct tgttgtcgag ctcgccattc
     1981 gcgccgatga ggtaggtctc gccgaccttg ccgtcgtcga tgatggtcca cactgcggag
     2041 ttgtggtcgt caacgtggat ccagtcgcgc acgttgagtc ccgcgccata gagcttgggc
     2101 cgaaccccgt cgaggatgtt cgtgatctgg cgggggatga acttctccac gtgctggagc
     2161 ggcccgtagt tgttcgagca gttcgagatc gtggcctcga cgccgaatga ccgcacccac
     2221 gcgcgcacga gaaggtcgga gcccgccttc gtcgccgagt aggggctgga agggttgtag
     2281 ggcgtgtcgg gggtgaactt ggccggatcg tcgagctcca ggtcgccgta
//
LOCUS       DASVKP010000123         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1280888, whole genome shotgun sequence.
ACCESSION   DASVKP010000123 DASVKP010000000
VERSION     DASVKP010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280888"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /locus_tag="VF362_02555"
     CDS             complement(<1..123)
                     /locus_tag="VF362_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEX7589838.1"
                     /translation="MTTTRPLVGIIMGSDSDWPVMEAAARALDEFGIPHEARVLS"
     gene            192..641
                     /locus_tag="VF362_02560"
     CDS             192..641
                     /locus_tag="VF362_02560"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX7589839.1"
                     /translation="MHLATWLRERRGELLRFGAVGGLAFVVDLGVLNLVYFVWRTPLA
                     HDQKVVTANVLSSIVATLVSWVGNRLWAFSHRRARHPVSELVLFAAVNGVALAERAAV
                     AAIGAYVLHFTSQTGINVAAIIGIGIGTITRYFGYRRFVFTGGRADA"
     gene            complement(616..>1783)
                     /locus_tag="VF362_02565"
     CDS             complement(616..>1783)
                     /locus_tag="VF362_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7589840.1"
                     /translation="RALEARYINGETISDAILESYVGGKVSPAAYILVTLPKGETFGA
                     GSRPARAIATQYVSNSGLKVEMEISWWDVFWSAARALLLVLGAGLVAIAAGVAVAVRQ
                     AGRLSEPLVLLAASAEQLGSGHSRPRIEKSGVEEIDLVAAELVRSGDRMAARLANERR
                     FSSDASHQLRTPLTSLSMRLEEISSTTTQEGVRAEAEAALVQVERLVGVVDDLLGRTR
                     KALGGSTEAVVLGKVLRQQREEWAPAFGSAGRTLTVGRTDATVFATPGGLAQVLATLI
                     ENSLAHGAGTTSVTVRESGATNAIVIDVSDEGAGVSAEIAPRIFERDVTSGRGTGLGL
                     ALARDLVTADGGRLELSQRAPAVFSVFLQAVPRMVDMEKVVPHSATLRRRRGRR"
BASE COUNT          276 a          659 c          569 g          279 t
ORIGIN      
        1 cgacagaact ctggcctcgt gaggtatccc gaactcatcg agcgcgcgcg cggccgcctc
       61 catcaccggc cagtccgagt ccgaacccat gatgatgccc accaggggcc gagtcgtcgt
      121 catgtccacc tccggctccc acgatagtgg caacgtcgtc gcgcgccgcc gacgcgggct
      181 aggctctcgc catgcacctc gcgacatggc tccgtgagcg acgcggcgag ttgctccgat
      241 tcggagcggt cggcggcctg gcgttcgtgg tcgatctcgg cgtcctcaac ctcgtgtact
      301 tcgtctggcg gacgcccctc gcccacgacc agaaggtcgt cacggcgaat gtcctgtcgt
      361 cgatcgtcgc caccctcgtc tcctgggtcg gcaatcggct ctgggccttc tcccaccggc
      421 gcgcgcgcca tcccgtgagc gaactcgtcc tcttcgcggc ggtgaacggc gtcgcgctcg
      481 ccgagagggc cgcggtcgcg gcgatcggcg cctacgtcct ccatttcacg agccagaccg
      541 gcatcaacgt cgcggcgatt atcggaatcg gaatagggac gatcacccgg tacttcggct
      601 accggcgctt cgtcttcacc ggcggccgcg ccgacgcctg agtgtcgccg agtgagggac
      661 gaccttctcc atgtccacca tccgcggaac ggcctgaagg aagaccgaga acaccgccgg
      721 cgcgcgctgc gagagttcga gcctcccgcc atctgcggtg acgaggtctc gtgcgagcgc
      781 caggccgagt ccggttccgc ggcccgacgt cacgtccctt tcgaatatcc gcggcgcgat
      841 ttccgcggaa acgccggcgc cctcgtccga gacgtcaatg acgatcgcgt tggtggcccc
      901 ggactcgcgc accgtcacgg acgtcgtgcc cgcgccatgc gcgagcgagt tctcgatgag
      961 ggtcgcgagg acctgagcga ggccgcccgg cgtcgcgaag accgtcgcgt cggtccgccc
     1021 cacggtcagg gtccttcccg cggacccgaa ggccggagcc cactcctcac gctgctggcg
     1081 aagaaccttg ccgagaacca ccgcttccgt cgagccgccg agcgccttcc tcgtcctgcc
     1141 gagcaggtcg tcgaccaccc cgacgaggcg ctccacctgc accagcgccg cctcggcctc
     1201 ggcgcgaacc ccctcctggg tcgtcgtcga cgagatctcc tcgaggcgca tgctgagcga
     1261 agtcagtggg gtgcggagtt gatgcgacgc gtccgacgag aaccgcctct cgttcgcgag
     1321 acgcgccgcc atgcggtccc cgctgcgcac gagctctgcc gcaacaagat cgatctcctc
     1381 gacccccgac ttctcgatac gcggtcgcga gtgacccgat cccagctgtt ccgcgctcgc
     1441 ggcgaggagc acaagcggct cggagagcct gcccgcctgg cggacagcga cggcgactcc
     1501 ggcggcgatg gcgacgagtc cggcgccaag gacgaggaga agcgcgcgag ccgccgacca
     1561 gaagacgtcc caccaggaga tctccatctc gaccttcaag ccggaattgg acacgtactg
     1621 tgtcgcaatc gcccgggccg gccgcgaacc cgccccgaac gtctcgccct tggggagcgt
     1681 gacgaggatg tatgccgccg ggctgacctt tccgccgacg tacgactcga gaatggcgtc
     1741 gctgatcgtc tcgccgttga tgtagcgcgc ctcaagcgcc cgc
//
LOCUS       DASVKP010000124         2689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1362066, whole genome shotgun sequence.
ACCESSION   DASVKP010000124 DASVKP010000000
VERSION     DASVKP010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362066"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /gene="hflX"
                     /locus_tag="VF362_02570"
     CDS             complement(<1..1374)
                     /gene="hflX"
                     /locus_tag="VF362_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX7589841.1"
                     /translation="MTSPSDDEMADDSPEAARSRDMVDRILSRAGTAVAAGGSTSTDY
                     DGDQFEREERAGMRRVAGISTELEDVSEVEYRALRLERVVLVGQWSRGSAEEAEVSMR
                     ELAALAETAGSEVLDAMLQRRAHPDPATYLGSGKAKELAGVVAAVGADTVVVNGDLSP
                     SQRRALEDVVRVKVVDRTAVILDIFAQHAKSKEGKAQVELAQLEYLLPRLRGWGDSMS
                     RQAGGRVAAGTGIGSRGPGETKIELDRRRIRTRMALLRRQIAHMAPAREVRRDRRTNL
                     PNVAIVGYTNAGKSSLLNRLTGAGVLVENALFATLDPTVRRAQTPEGREFTLSDTVGF
                     VRDLPHQLVAAFESTLEEVAHADLVLHVVDAAHPDPEGQILAVRTVLAGVPGAGSVPE
                     ILVLNKCDVASADTIAGLRARRDITVEVSALTGQGMGDLLRIIAENIPEPAVRVDVVV
                     PYTRGDLV"
     gene            1423..2088
                     /locus_tag="VF362_02575"
     CDS             1423..2088
                     /locus_tag="VF362_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7589842.1"
                     /translation="MKDDRGGVGDDGAAGDHYFTARPASPDQRREISTTLAGIGVAVE
                     TAPGVFSSSRVDLGTSVLLRTVPSPPSGTLLDLGCGWGPIALTMALLQPRARVWAVDV
                     NERALDLVRRNAVRVAQIAPASEVIAATPDEVPDDLAFDAIWSNPPVRIGKDALHDLL
                     ARWLPRLAPGAEAYLVVQRNLGADSLARWLADQRDDADLAWGAVEKVASAKGFRVLRL
                     RRA"
     gene            complement(2085..>2689)
                     /locus_tag="VF362_02580"
     CDS             complement(2085..>2689)
                     /locus_tag="VF362_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX7589843.1"
                     /translation="GNGARVFVAYLEREAGEDAAEGIVIATRGGPHTVTALGGSRYAV
                     DMGAWRLDAEGGPDVAVRGWNARPAVSVSMTNPHAVIAALTVEELAAADLSVAPSVEP
                     APPAGVNVELVVPLNLDGARGRLRMRVHERGVGETRSCGTGACAAALAARWWSGDSAP
                     DVWDVEVPGGAVVVRIEEGRTVLEGPAVLVADGVLDLRSI"
BASE COUNT          356 a         1009 c          939 g          385 t
ORIGIN      
        1 caccaagtcc ccgcgcgtgt atggaacgac gacgtcgacg cgcaccgcgg gctcggggat
       61 gttctcggcg atgatccgga gcaggtcgcc catcccctgc cccgtgagcg ccgagacctc
      121 gacggtgatg tcgcggcgcg cccgaaggcc cgcgatcgtg tccgcgcttg ccacgtcgca
      181 cttgttgagc acgaggatct cggggacgct ccccgccccc ggcaccccgg caaggacggt
      241 cctgaccgca aggatctggc cctcgggatc ggggtgcgcg gcgtcgacca cgtggaggac
      301 gaggtcggcg tgcgcgacct cctcgagcgt cgattcgaac gccgcgacga gttgatgggg
      361 aaggtcgcgg acgaacccga cggtgtcgga gagggtgaac tcgcgccctt ccggcgtctg
      421 ggcacgccgg acggtcgggt cgagggtcgc gaagagcgcg ttctccacga gcacgcccgc
      481 gcccgtcagg cggttgagga gcgacgactt cccggcgttg gtgtagccga cgatcgcgac
      541 gttcggcagg ttcgtccgcc ggtcgcgtcg gacctcgcgc gcaggcgcca tgtgggcgat
      601 ctggcgcctg aggagcgcca tgcgggtgcg gatgcgccgg cggtcgagtt cgatcttggt
      661 ctcgccgggg cctcgcgagc cgatccccgt gcccgccgcg acccgtccgc ccgcctggcg
      721 ggacatcgag tcgccccacc cgcggagcct cggaagcagg tattcgagct gggcgagttc
      781 gacctgcgcc ttgccctcct tggacttggc gtgctgcgcg aagatgtcaa ggatcactgc
      841 ggtgcggtcc acgaccttga ccctcacaac gtcctcgagc gctcggcgct ggctcgggga
      901 gaggtccccg ttcacgacga cggtgtccgc cccgacggcg gcgacgacgc cagctagttc
      961 cttcgccttc ccggaaccca ggtacgtcgc gggatcgggg tgggcgcgcc gctggagcat
     1021 cgcgtcgagg acctcggagc ccgccgtctc tgcgagcgcc gcgagttccc tcatcgagac
     1081 ctcggcctcc tccgccgatc cccgcgacca ctggccgacc agcaccaccc gctcgagccg
     1141 gagggccctg tattcgacct cggaaacgtc ctcgagctcc gtggagatgc cggcgacgcg
     1201 gcgcatcccg gcgcgctcct cgcgctcgaa ctgatccccg tcgtagtccg tgctcgtcga
     1261 gccccccgcc gcgaccgccg tccccgcccg cgagaggatg cggtcgacca tgtcgcgcga
     1321 gcgcgcagcc tcgggcgaat cgtcggccat ctcgtcgtcg gaaggggagg tcatggttgg
     1381 cccattgtct caccctgccc cgacgcgggt acgctcgctc ccgtgaagga tgaccggggt
     1441 ggggttggtg acgacggcgc ggcgggagat cactacttca ccgcgaggcc tgcgtcgccc
     1501 gaccagcgca gggagatctc cacgacgctc gcgggcatcg gcgtcgcggt cgagaccgcg
     1561 ccgggcgtct tcagctcgag ccgcgtcgac ctggggacat cggtgctcct tcgcacggtt
     1621 ccctcgcccc cctcggggac gctcctcgac ctgggttgcg gctggggccc catcgccctg
     1681 acgatggccc tgcttcaacc ccgggcgcgg gtgtgggcgg tggacgtcaa cgagcgagcg
     1741 ctcgacctgg tccgcaggaa cgccgtgcgc gtcgcccaga tcgcccccgc cagcgaggtg
     1801 atcgcggcca cgcccgacga ggttcccgac gatctggcgt tcgacgcgat ctggtcgaac
     1861 cctcccgtac ggatcggcaa ggacgctctc cacgacctcc tcgcgcggtg gcttccccgg
     1921 ctggcccccg gcgccgaggc ctacctcgtc gtccagcgca acctcggcgc cgattcgctc
     1981 gcgcgatggc tcgccgacca gcgcgacgac gcggacctgg catggggagc ggtcgagaag
     2041 gtggcgagcg cgaagggatt ccgggtcctg cggctccgcc gcgcctagat ggaccttagg
     2101 tccaggaccc cgtcggccac caggacggcc ggaccctcga ggacggtcct cccctcctcg
     2161 atgcggacga cgacggcgcc gccgggcacc tcgacgtccc acacgtccgg ggcggagtcc
     2221 cccgaccacc accgcgcggc gagggccgcg gcacacgccc cggttccgca cgagcgcgtc
     2281 tcgccgaccc cgcgctcgtg gacgcgcatc ctcaggcgcc cgcgggcccc gtcgaggttc
     2341 agcggaacca cgagctcgac gttgacgccc gcggggggcg ccggctccac cgacggggcg
     2401 acagagaggt cggccgccgc cagttcctcc accgtcagcg ccgcgatcac cgcgtgcgga
     2461 ttggtcatgc tgaccgagac cgctggcctg gcgttccagc cgcggaccgc cacgtccggg
     2521 ccgccctccg catcgagtcg ccatgcgccc atgtcaacgg cgtagcgcga gcctccgagc
     2581 gcggtcacgg tgtgcgggcc gccgcgcgtc gcgatgacga tgccctcggc cgcgtcttcg
     2641 ccagcctcgc gctcgaggta cgcgacgaac acgcgcgcgc cgttcccgc
//
LOCUS       DASVKP010000125         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049 k141_42410,
            whole genome shotgun sequence.
ACCESSION   DASVKP010000125 DASVKP010000000
VERSION     DASVKP010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42410"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1221)
                     /locus_tag="VF362_02585"
     CDS             complement(115..1221)
                     /locus_tag="VF362_02585"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX7589844.1"
                     /translation="MGRPTVAVLFGGRSSEHGVSCVTASGVLRAIDRVRWDVLAVGIA
                     RDGQWTLANDDPDDWRIREGELPSVVGGGATVLPPFRAGDAMWRVLRDGGALEGGAVE
                     DLAAVDVVFPLLHGPWGEDGTVQGALDLVDVRYVGSGVLASALGMDKAFAKMAFRAAG
                     LPVANAVVIDGDGDVALRRSEIEALRLPVFVKPARAGSSQGVSRVASWDALDAALASA
                     LKHDPKVLIEEGIAGREIECAVLETPDGVRASQPGEIVTRGDHEFYDYEAKYFDEAAV
                     TLACPAELPPGVSEVVRDYALRAFAAIDGEGIARADMFVLSDGSVIVNEINTMPGFTP
                     FSMYPLMWEVEGLDYRALVETLLTTALERPVGLR"
     gene            complement(1225..>2052)
                     /locus_tag="VF362_02590"
     CDS             complement(1225..>2052)
                     /locus_tag="VF362_02590"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX7589845.1"
                     /translation="GRATGDAGEALAGADLVFLALPAQSARAIVAEHASLVPRGAVVA
                     SLMKGIEVGSHLRMSEVLAEVWGIDEERLAVVSGPNLATEIALRQPTATVVASTSAET
                     AKRVADAVASAYFRPYTNPDVVGVELCGAYKNVIAVSVGIADGRGFGNNTTATIITRG
                     LAEMARLGLALGASAETFPGLAGMGDLIATCASPLSRNHTLGSHIGQGYTLREAIAAT
                     KGTAEGVETSRSIQQLADEHGVDIPICNAVVKMLHGGADPETIMSALLSRPRKAEGA"
BASE COUNT          318 a          736 c          713 g          285 t
ORIGIN      
        1 aaggtcagcg ccgacggcac ggagcagatg ctccgatccg cgtacgccaa ggtcaagtag
       61 gcggagcgcc gcggtcgccc gcaagggcgg ccgcggccgt tccctcccgt cgatctagcg
      121 aagtccgacg gggcgctcga gggccgtggt gaggagcgtc tccacgaggg cgcggtagtc
      181 gagcccctcc acctcccaca tgagcgggta catcgagaag ggcgtgaagc ccggcatcgt
      241 gttgatctcg ttgacgatga cggatccatc cgacaggacg aacatgtccg cccgcgcgat
      301 tccctcgccg tcgatcgccg cgaaggcacg cagggcgtag tccctgacca cctcgctgac
      361 gcccggtggt agctccgccg ggcacgcgag cgtcacggcg gcctcgtcga agtacttggc
      421 ctcgtagtcg tagaactcgt ggtctccccg cgtgacgatc tcacccggct ggctcgcgcg
      481 aacgccgtcg ggcgtctcga gcacagcaca ctcgatctcg cggcccgcga tcccctcctc
      541 gatgagcacc ttgggatcgt gcttgagggc cgatgctagc gccgcgtcga gcgcgtccca
      601 cgaggccacc cggctgacgc cctgggaaga ccccgcgcgg gcgggcttga cgaagacggg
      661 gaggcgcaga gcctcgattt cggaacgcct gagcgcgacg tcgccgtcgc cgtcgatcac
      721 cacggcgttg gcgacgggca gccccgcggc gcgaaacgcc atcttggcga acgccttgtc
      781 catcccgagg gccgacgcca ggaccccgga ccccacatac cgcacgtcca cgaggtcgag
      841 cgcgccctga accgtgccgt cctcgcccca cgggccgtga aggaggggga acacgacgtc
      901 gacggcggct aggtcttcga cggcaccgcc ctcgagggcg cctccgtcgc gaagcacgcg
      961 ccacatggcg tcgccagcgc ggaacggcgg aaggacggtg gcgccgccgc ccacgaccga
     1021 aggcagttcg ccctcgcgga tgcgccagtc gtcgggatcg tcgttcgcga gggtccactg
     1081 gccgtcgcgc gcgatgccga cggccagcac gtcccaccgg acccgatcga tcgcgcgaag
     1141 gaccccggag gcggtcacgc acgagacgcc gtgctccgag gatctccccc caaagaggac
     1201 ggcaaccgtt gggcgaccca tggcctacgc cccctcggcc ttgcggggcc gcgaaagaag
     1261 cgcggacatg atcgtctcag ggtccgcgcc gccgtgaagc atcttgacca cggcgttgca
     1321 gatcgggatg tcgacgccgt gctcgtctgc gagttgctgg atcgagcgcg acgtctccac
     1381 accctcggcg gtacccttcg tcgccgcgat cgcctcgcgg agagtgtagc cctggccgat
     1441 gtgggacccg agcgtgtggt tcctcgacag gggcgaggcg cacgtcgcga tgaggtcgcc
     1501 catgcccgcg aggccgggga aggtctcggc ggaggcccca agcgcgaggc caaggcgagc
     1561 catctccgcg aggccgcggg taatgatcgt ggccgtggtg ttgttgccga agccgcgtcc
     1621 gtcggcgatc cccacgctca cggctatgac gttcttgtat gcgccgcaca gttccacgcc
     1681 gacgacatcc gggttcgtgt acgggcggaa gtacgcggac gcgacagcgt cggcgacgcg
     1741 cttcgccgtc tcggcggagg tcgacgccac gaccgtggcg gtgggctgcc ggagcgcgat
     1801 ctcggtcgcc aggtttggcc ccgagaccac ggcgagccgt tcctcgtcga tcccccacac
     1861 ctcggcgagc acctcgctca tcctcaggtg cgagccgacc tcgatgccct tcatcaggga
     1921 tgcgacgacg gcgcctcgcg gaacgaggga ggcgtgctcg gcgacgatcg ccctagcgct
     1981 ctgcgcgggc agggcgagga acaccaggtc ggcgcccgcg agcgcctcgc ccgcgtcgcc
     2041 cgtcgcgcgc cc
//
LOCUS       DASVKP010000126         6413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_705184, whole genome shotgun sequence.
ACCESSION   DASVKP010000126 DASVKP010000000
VERSION     DASVKP010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705184"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="VF362_02595"
     CDS             complement(<1..1318)
                     /locus_tag="VF362_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX7589846.1"
                     /translation="MTDPAHLAWGETWPTAEGFVELGRTHRVVPVVRKVLGDSVTPVA
                     AYRALAAERPGTFILESADPSGTRAQFSFVGVRSRAVLTATGSDARWTGDVPLGLGDG
                     APLEVLREVLAEMRSETIDGLPPLTGGLVGLLGWDIIRQWEPTLPANAPREIDVPDVL
                     LLFATDLAAIDHHDGSVWFIANAVNADAQETGIEAAYADAVARIDAMEAALRDAPTGD
                     MSVMDESGVDPRITDRSAPGEYERVVAVCKEAIRDGEVFQVVPSQRFDVECSASPLDV
                     YRVLRTLNPSPYMYYFQLRDGEGRDFAVVGSSPESLVAVKGDEVMTYPIAGSRPRGAT
                     PEEDALLQKDLLADPKELAEHVMLVDLSRNDLVKVCAPTSVEVVEFMAVNRYSHIMHI
                     CSTVVGRLEAPHTALDAFVATFPAGTLSGAPKPRAIALIDEVEPARR"
     gene            complement(1315..1683)
                     /gene="hisI"
                     /locus_tag="VF362_02600"
     CDS             complement(1315..1683)
                     /gene="hisI"
                     /locus_tag="VF362_02600"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX7589847.1"
                     /translation="MALDPGIVGRLKRDAHGLVCAVVQQHDTGEVLMVGWMNDEALEQ
                     TLTTGRAVFWSRSRHEIWRKGDTSGNIQRIRAVSLDCDGDALLLSVDQVGPACHTGER
                     SCFDAGGDLGAVDVVTGAGE"
     gene            complement(1713..1871)
                     /locus_tag="VF362_02605"
     CDS             complement(1713..1871)
                     /locus_tag="VF362_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589848.1"
                     /translation="MIAHHLSTAERADIVGVMESGNLVELGMHRELAKAGGTYSQLYD
                     AWVTQTRD"
     gene            complement(1868..2638)
                     /gene="hisF"
                     /locus_tag="VF362_02610"
     CDS             complement(1868..2638)
                     /gene="hisF"
                     /locus_tag="VF362_02610"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX7589849.1"
                     /translation="MGVAVRVIPCLDVDAGRVVKGVNFENLRDAGDPVELARHYGDSG
                     ADEVTFLDVSASSSGRETMLDVVRRTADQVFVPLTVGGGVRSVEDVDRLLRAGADKVG
                     VNTAAIARPELIAEIAARFGNQVLVLSVDARRCGNGAATPSGYEVTTHGGRRGTGIDA
                     VQWAARGAELGAGEILLNSMDADGTTGGFDLAMLRDVRAVVTVPLIASGGAGKAEDFV
                     TAALSGADAVLAASVFHFGIISIGEVKRAMRDAGIEIR"
     gene            complement(2644..3510)
                     /locus_tag="VF362_02615"
     CDS             complement(2644..3510)
                     /locus_tag="VF362_02615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7589850.1"
                     /translation="MPRIVAVLVGALFALGGCAGAPGADSSAASIDNIVVGHDATGSP
                     TIEYPAGGNFTQAQSRVEWEGTGERLTEGDPVLLDLYSVSLTTGAILTDTYLDLPQAF
                     LVAPELLGNDLYGAIVGHKVGTRVLLVVPESPTHPGQGTVAVVADVLPERAIGVTQPA
                     RADLPIVIDGKYGEPTVTFRDDVEVPTDLVAYTLIQGGGPQIKTGSRILLNYQETSAT
                     TREVVQSTWPVEIAPWAVTIGQGEVPTGLEQSLIDVNEGSQIIVIVPPALAYGDDTLI
                     FVVDVLAVRNPG"
     gene            complement(3539..4963)
                     /gene="pafA"
                     /locus_tag="VF362_02620"
     CDS             complement(3539..4963)
                     /gene="pafA"
                     /locus_tag="VF362_02620"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HEX7589851.1"
                     /translation="MTATEVPPPLEVPASRRIFGLETEFGLHFRTEAERKVTSEEVAG
                     ALFHSVVAWGRSSNVFLTNGSRLYIDVGAHPEYATAECDRLEDLIALDKAGERIVGAL
                     VADGERRLAEAGVVGDIHLYKNNSDSAGNSYGCHENYLVRRRSDFTSFAESLLPFLVT
                     RQILTGSGCVTGTGATARYCFSPRADHMWEAMSSATTRSRPMINTRDEPHAHADLYRR
                     LHVIVGDSSMAEPTALLKVGATDLILRLLEARVVMPALALAREMQAIRDVSHDMSGAA
                     TVELADGRRLTAVEIQRQYLDLVRSHVGGIIEPDTTTERILDLWARGIEAVRTGDSSA
                     VWTELDWAIKLRLVERLRGRTGAPLDDPRVARLDLAYHDVAPGRGLGSSLVAQGLMAT
                     VVPEEAIVRAVERPPETTRAKLRGDFVRAGIGAHRDFTADWVRLKVVGDEGRTVLLKD
                     PFRSEDARVDALIASIAPLESRVD"
     gene            complement(4972..5157)
                     /locus_tag="VF362_02625"
     CDS             complement(4972..5157)
                     /locus_tag="VF362_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEX7589852.1"
                     /translation="MPQVTSGEQPYDDVPEPEPPTPGGAPQAQTDAVDALLDEIDVTL
                     QANAEQFVRSFVQKGGE"
     gene            complement(5167..>6413)
                     /gene="dop"
                     /locus_tag="VF362_02630"
     CDS             complement(5167..>6413)
                     /gene="dop"
                     /locus_tag="VF362_02630"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HEX7589853.1"
                     /translation="GSWEDPSHLNAVLSNGARLYVDHAHPEYSGPEVTTARDAVLWDR
                     AGDEVARRAAIAASFDGFSVALYKNNVDGKGASYGTHENFLVERSVPFDELARRITAH
                     LVTRQVFTGAGRVGLGQRGEEPGFQISQRADYMEAEVGLETTLRRPIVNTRDEPHADP
                     ARWRRLHVIIGDANCMDVPTMLKVGTTALVLSAIEAGDARLDVLELGDPVEAVYAVSR
                     DLSLGRRLPLASGASASALEIQRALLEIARDYATDAGDAEVLARWEGVLERLARDPFA
                     CAADVEWVAKLQFLGRLRARHGSAEEPLPWDDPRLQAADVQWSDLDPARGLCSRLAVA
                     GAVERLVTDAQIAQAVTTPPSTTRAWLRGRMVAARPEIVDAAGWSTIVLDRETSHGEL
                     EVVTLNDPLATTHPLLDSLLAP"
BASE COUNT          988 a         2244 c         2192 g          989 t
ORIGIN      
        1 ccctgcgtgc gggctccacc tcgtcgatca acgcgatggc gcggggcttc ggcgctccgg
       61 acagggttcc cgccggaaag gtcgcgacga acgcgtcgag tgcggtgtgg ggcgcctcga
      121 gccgccccac cacggtggaa caaatgtgca tgatgtggct gtagcggttc acggccatga
      181 actcgacgac ctcgaccgat gtcggggcgc agaccttcac caggtcattg cgtgacaggt
      241 ccacgagcat gacgtgctcg gcaagctcct tggggtccgc cagaaggtcc ttctggagga
      301 gggcgtcctc ctcgggcgtc gcgccgcggg gccgcgatcc cgcgatggga tacgtcatca
      361 cctcgtcgcc cttcacggcc accagcgact ccgggcttga cccgaccacg gcgaagtccc
      421 tgccttcgcc gtcgcgaagt tggaagtagt acatgtacgg gctcgggttc agggtgcgca
      481 ggacgcggta gacgtccagc gggctcgcag agcactccac gtcgaacctc tgcgacggga
      541 ccacctggaa gacctcgccg tcccggatag cctccttgca gacggcgacg accctctcgt
      601 actcccccgg cgcggagcgg tccgtgatcc gcggatcgac tcccgattcg tccatcaccg
      661 acatgtcacc cgtcggggcg tcacgcagcg cggcctccat ggcgtcgatc cgagcgacgg
      721 catcagcgta cgcggcctcg atccccgtct cctgggcgtc cgcgttgacc gcgttggcga
      781 tgaaccagac gcttccgtca tggtgatcga tcgcggcgag atcggtcgcg aagagcagga
      841 ggacgtccgg gacgtcgatc tcgcgcgggg cgttcgcggg aagcgtgggc tcccattggc
      901 ggatgatgtc ccatccgaga agcccgacga gtccgccggt aagcggcggg aggccgtcga
      961 tggtctccga gcgcatctcc gcgagcacct ctcgcagaac ctcgaggggg gcgccgtcgc
     1021 ccaggccaag gggaacgtcg ccggtccagc gtgcgtccga accggtcgcg gtaagcacgg
     1081 cgcgggaccg caccccgacg aacgagaact gggcgcgagt gccgcttggg tccgcggact
     1141 cgaggatgaa cgtgccgggg cgttcggctg cgagcgcgcg gtacgcggcg acgggggtga
     1201 ccgagtctcc cagtaccttc cgcaccaccg gcacaacgcg gtgggttcgg ccgagttcaa
     1261 cgaagccctc tgcggtgggc cacgtctctc cccaggcgag gtgcgccggg tccgtcactc
     1321 cccggccccc gtgacgacgt cgacggcgcc gaggtcgcct ccagcgtcga agcaggacct
     1381 ctcccccgtg tggcacgcgg gcccgacctg atcaaccgag aggagaaggg cgtctccgtc
     1441 gcagtcgagg gacaccgcgc ggatccgctg gatgttgccc gacgtgtcgc ccttgcgcca
     1501 gatctcgtgg cggcttcggc tccagaacac ggcgcggccc gtcgtgagcg tctgctcgag
     1561 cgcctcgtcg ttcatccatc ccaccatcag cacctcgccc gtgtcgtgct gctggacgac
     1621 ggcgcacacg aggccgtgcg cgtcgcgctt gaggcggccg acgatgcctg ggtcgagagc
     1681 catggggcca tcattccacg gttcaccccg cgtcagtccc tggtctgagt cacccatgcg
     1741 tcgtacaact gggagtaggt cccccccgcc ttcgccagct ccctgtgcat cccgagttcc
     1801 accagattgc ccgattccat caccccgacg atgtcggcgc gctccgcggt cgagaggtgg
     1861 tgggcgatca tcgaatctcg attcccgcgt ctcgcatggc gcgcttgacc tcgccgatag
     1921 agatgatgcc gaagtggaac acgctcgccg caagaaccgc atcggccccc gagagtgccg
     1981 ccgtcacgaa gtcttcggcc ttgcccgctc ctcccgacgc gatgagcggg accgtgacga
     2041 ccgccctcac gtcccgaagc atggcgaggt cgaagccccc cgtcgtgccg tcggcgtcca
     2101 tcgaattcag gagaatctcc cccgcgccaa gttcggcgcc gcgcgcggcc cattggacgg
     2161 cgtcgattcc cgtgccccgt cgcccgccgt gggtcgtcac ctcgtagccc gatggagtcg
     2221 cggcgccgtt cccgcacctg cgcgcgtcga cggacaagac gagcacctgg ttgccgaagc
     2281 gcgccgcgat ctcggcgatc agttcggggc gcgcgattgc ggccgtgtta acgccgacct
     2341 tgtcggcgcc ggcgcggagc agtcggtcca cgtcctcgac cgaccgaacc ccacccccca
     2401 ccgtcagggg gacgaagacc tggtccgccg tgcgcctgac gacgtccagc atggtctcgc
     2461 gccccgagga ggacgccgag acgtcgagga acgtcacctc gtccgccccg gagtcgccgt
     2521 agtggcgcgc gagctcgacg ggatctcccg cgtcgcggag gttctcgaag ttcacgccct
     2581 tcacgaccct gcccgcgtcg acatccaggc aaggaatcac ccgcaccgcg acgcccattg
     2641 ccgctacccc ggattccgca cggcgaggac gtccacgacg aatatcaacg tgtcgtcgcc
     2701 ataggccagg gcggggggca cgatcacgat gatctgggag ccctcgttta cgtcaatcag
     2761 gctctgctcg agtcccgtgg gcacctcgcc ttggccgatc gtcacggccc agggcgcgat
     2821 ctccacgggc cacgtcgact gcacgacctc gcgggtcgtg gccgaggtct cctggtagtt
     2881 cagcaagatg cgggagcccg tcttgatctg gggcccgccc ccctgaatca gcgtgtacgc
     2941 caccaggtcg gtcggcacct cgacgtcgtc gcggaacgtg accgtcggtt cgccgtactt
     3001 cccgtcgatc acgatcggga gatccgcgcg agcgggctgg gtcactccga tcgcacgctc
     3061 cggcaacacg tccgcgacca cggcgaccgt tccctgccca gggtgcgtcg gcgactccgg
     3121 aacgacgagg agcacccgcg tgcccacctt gtgcccgacg atcgcgccat acaggtcgtt
     3181 gccgagcagc tcgggcgcca cgagaaacgc ctgtggaaga tcgaggtagg tgtctgtgag
     3241 gatggcccca gtcgtgaggg agaccgagta gagatcgagc agaacggggt cgccctcggt
     3301 cagccgctcg cccgtgccct cccactccac ccgcgactgc gcctgcgtga agttgcctcc
     3361 cgccgggtac tcaatcgtgg gcgaacccgt agcgtcgtgc ccgacaacga tgttgtcgat
     3421 cgaggccgcc gaggagtcgg cgcccggggc gcccgcacat ccaccgagcg cgaagagcgc
     3481 accaacgagc acggcgacaa tccgcggcat ggcctgtcct cctgaggggt tcgggcgctc
     3541 agtctacgcg ggactcgagg ggcgcgatcg aggcgatcag ggcgtcgacg cgcgcgtctt
     3601 cggagcgaaa cggatccttg agcaggacgg tgcgcccctc gtcgccgacg accttgagcc
     3661 gcacccagtc ggcggtgaag tcccggtgcg cgccgatgcc cgcgcgaacg aagtccccgc
     3721 gcaacttcgc tcgcgtcgtc tccggcgggc gctccacggc gcgaacgatg gcctcctccg
     3781 gcacgacggt cgccatcagg ccctgggcca cgaggctgga tccgagcccc cgcccgggcg
     3841 cgacgtcgtg ataggcaaga tctaggcgcg cgaccctcgg atcgtcgagc ggcgcccccg
     3901 tccgacctcg caagcgttca acgagccgca acttgatcgc ccaatcgagc tccgtccaga
     3961 ccgccgacga gtcgcccgtg cgcacggctt cgatgccccg cgcccagagg tcgaggatcc
     4021 gctccgtcgt tgtgtcgggc tcgatgattc ccccgacgtg cgaccgaacg aggtcgaggt
     4081 actgccgctg gatctcaacg gcggtcaaac gccgaccgtc cgcaagttcg acggttgccg
     4141 cacccgacat gtcgtgagag acgtcccgga tcgcctgcat ctctcgcgcg agcgccaggg
     4201 cgggcatcac gacccgggcc tcgagcaggc gaaggatcag atcggtggcg cccaccttga
     4261 ggagcgcggt cggttcggcc atcgacgagt cgccgacgat gacgtgaagc ctcctgtaga
     4321 ggtccgcgtg ggcgtgaggc tcgtcgcggg tgttgatcat gggccgcgag cgcgtcgtcg
     4381 ccgaggacat ggcctcccac atgtgatcgg cgcgcggcga gaagcagtag cgcgcggtgg
     4441 ctccggttcc cgtcacgcag ccggacccgg tgaggatctg gcgcgtgacc aggaagggaa
     4501 gcagggattc cgcgaaagac gtgaaatccg atcggcggcg cacgaggtag ttctcgtggc
     4561 atccgtagga gttcccggcc gagtcggagt tgttcttgta gaggtggatg tcgcccacga
     4621 cgccagcctc ggcaaggcga cgctcgccat cggcgaccag cgccccgacg atcctctccc
     4681 ccgccttgtc gagcgcgatg aggtcttcga gccgatcgca ctccgcggtt gcatactccg
     4741 ggtgcgcgcc gacgtcgatg tacaggcgcg agccgttggt gaggaagacg tttgaggagc
     4801 gaccccacgc gacgaccgag tggaacagcg cccccgcgac ctcctcggag gtgaccttgc
     4861 gctcggcctc tgtgcggaag tggagcccga attccgtctc cagcccgaat atccggcgcg
     4921 aggcaggaac ctcaaggggc ggcggaacct cggtggctgt cacgggatcg gctactcgcc
     4981 gcccttctgg acgaagctcc tgacgaactg ctccgcgttg gcctggagcg tcacgtcgat
     5041 ctcatcgagc agagcgtcga cggcgtcggt ctgcgcctgc ggggcgcctc ccggcgtcgg
     5101 tggttccggc tcggggacgt cgtcgtaggg ctgctcgccg ctcgtgacct ggggcatgcg
     5161 cccagtctat ggggcgagca gggagtcgag gagcggatga gtggtcgcga gcgggtcatt
     5221 gagggtgacg acctcgagtt ccccgtggct cgtctcgcga tcgaggacga tggtgctcca
     5281 ccccgcggcg tcgacgatct ccggccgcgc cgcaaccatg cgcccgcgga gccacgcgcg
     5341 cgtggttgat ggcggagtgg tcaccgcctg ggcaatctgc gcgtccgtca cgaggcgttc
     5401 cactgcgccc gcaactgcga gccgcgagca cagcccgcgc gcggggtcga ggtcgctcca
     5461 ctggacgtcg gcggcctgca ggcgcgggtc gtcccatggg aggggctcct cggcgcttcc
     5521 gtgccgggcg cgcaggcgac cgaggaattg gagcttcgcg acccactcaa cgtctgccgc
     5581 gcaggcgaag ggatcccgag cgagccgctc gaggacgccc tcccaccgcg cgaggacctc
     5641 cgcgtccccc gcgtccgtcg cgtaatcgcg cgcgatctcg aggagcgcgc gctggatttc
     5701 gagcgcactc gcggatgccc cggaggcgag cggcaggcga cgcccgagcg agaggtccct
     5761 ggagaccgcg tagacggcct cgaccggatc gccaagttcc agcacgtcga gtcgcgcgtc
     5821 ccccgcctcg atcgcggaga ggacgagggc agtcgtgccg accttgagca tcgtcggaac
     5881 gtccatgcag ttggcgtcgc cgatgatgac gtggaggcgc ctccagcgag cgggatcggc
     5941 gtgcggctcg tccctcgtgt tgacgatcgg ccgcctcagg gtcgtctcga ggccaacctc
     6001 ggcctccatg tagtccgcgc gctgggagat ctggaagccc ggctcctcgc cgcgctgacc
     6061 gagtcctacc cggccggcgc cggtgaagac ttggcgcgtc acgaggtgag cggtgatgcg
     6121 ccgcgcgagt tcgtcgaagg gcacggaccg ctcgacgagg aagttctcgt gcgttccgta
     6181 cgaggcgccc ttgccgtcca cgttgttctt gtagagggcg accgagaacc cgtcgaagga
     6241 ggccgcaatc gcagcccggc gcgccacctc gtcccccgcc ctgtcccaca gcaccgcgtc
     6301 ccgcgcggtg gtcacctcgg ggccggagta ctccgggtgg gcgtggtcaa cgtagaggcg
     6361 agccccgttg ctcagcacgg cgttgaggtg ggagggatcc tcccatgagc cac
//
LOCUS       DASVKP010000127         1724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1282347, whole genome shotgun sequence.
ACCESSION   DASVKP010000127 DASVKP010000000
VERSION     DASVKP010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282347"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VF362_02635"
     CDS             <1..1063
                     /locus_tag="VF362_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7589854.1"
                     /translation="NTAGALMIGVLLLALLTTVSTSITSLAKKSLEGSSSATFIVGGD
                     FTTDPLAPISDKDIAAMRAVKGVTDVHFYGSDTALIDGDPQMIVAIDAQSAEGAYEYK
                     VEPSFSQLADGHVYVSPGVLAAGKKLGDTVTIEGVDATRDFVISGTYLQEGDPSYWIT
                     FDDARILHKGLAATNSWVAISDTADVAKVQTALKEALAGNNRIQVSTIDDYTKKINQL
                     FNQVLAMMSAMLSMALFIAVFGVANTLFLSVTERTREIGLIRAVGVKRSGIWAMITLE
                     SVFVSLLGAILGLVLGVVFGVALVQSFDIFREFGITIPWIQLIVYTILAVIAGIIAAI
                     WPSWKASRLNILDAIATGE"
     gene            complement(1177..>1724)
                     /locus_tag="VF362_02640"
     CDS             complement(1177..>1724)
                     /locus_tag="VF362_02640"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX7589855.1"
                     /translation="RPTPRFVVLDVSFQMRRGTLSAPLQYGQLADVLGIEVGARAPLA
                     VVRDAVLALRRSKGMVLDPDDHDTWSAGSFFTNPILAAGAAAQLPQDAPRYPGGDAAP
                     TDAVKTSAAWLIEHAGFGRGFALPGSRAALSTKHTLALTNTGGASGDEVLALARHIRD
                     GVRRAFGVALEAEPVLVGVEI"
BASE COUNT          290 a          579 c          567 g          288 t
ORIGIN      
        1 aaacaccgcc ggcgcgttga tgatcggagt tctcctcctc gccctgctga cgacggtgtc
       61 gacgtcgatc acgagcctcg cgaagaagtc ccttgagggg tcctcgtcgg cgacgttcat
      121 cgtgggcggc gacttcacga cggatccgct tgcgccgatt tccgacaagg acatcgccgc
      181 gatgcgggcc gtcaagggcg tcaccgacgt tcacttctac ggttcggaca cggcactgat
      241 cgacggcgac ccgcagatga tcgtcgcgat cgacgcccag tcggcggagg gagcgtacga
      301 gtacaaggtc gagccgtcct tctctcaact cgccgacggg cacgtgtacg tgagtcccgg
      361 agtgctcgcc gccggaaaga agttaggcga caccgtcacc atcgagggcg tcgacgcaac
      421 ccgggacttc gtcatctcgg gcacgtacct ccaggagggc gatccgagtt actggatcac
      481 cttcgacgac gcgcggatcc ttcacaaggg cctcgcggcg acgaactcct gggtagcgat
      541 ctctgacacg gcggatgtcg ccaaggtcca gaccgcactc aaggaggcgc tcgcgggcaa
      601 caaccgcatc caggtgtcga cgatcgacga ctacaccaag aagatcaacc agctcttcaa
      661 ccaggtcctc gcgatgatgt ccgccatgct ttccatggcc ctctttatcg cggtgttcgg
      721 ggtggccaac acgctcttcc tgagcgtcac cgagcgcacc cgagagatcg gcctgatccg
      781 ggcggtcggc gtcaagcggt cggggatctg ggcgatgatc acgctcgaat cggtgttcgt
      841 gtcgcttctc ggcgcaatcc tggggctcgt ccttggggtc gtgttcggag tggcgctcgt
      901 tcagtcgttc gacatcttcc gggagttcgg aatcacgatt ccgtggatcc agttgatcgt
      961 ctatacgatc ctcgccgtga tcgctggcat catcgcagcc atctggccgt cgtggaaggc
     1021 gtctcgcctc aacatcctcg acgccatcgc gacgggcgag tagcggccgg agcgcggcga
     1081 ggggccccct gccgagaggc acggggcctc tcgccgttcc cgggtcctgg cgccgctcct
     1141 cgaactcgaa cccgctccta gaactcgacg acgaccctag atctcgacgc cgacgaggac
     1201 gggctccgct tcgagcgcga ctccgaaggc gcggcgcacg ccatcgcgga tgtgtcgcgc
     1261 gagcgcgaga acctcgtcgc ccgacgcgcc ccccgtgttc gtgagagcga gggtgtgctt
     1321 cgtggagagc gcggcgcggg agcccggcag ggcgaaaccc cgtccgaagc ccgcatgctc
     1381 gatgagccac gcggcggagg tcttcactgc atcggtgggg gctgcatcgc cccccggata
     1441 gcgcggggca tcctgcggga gctgcgcggc cgcacccgcg gcgaggatcg gattcgtgaa
     1501 gaacgacccc gcgctccacg tgtcgtggtc gtccggatcc aggaccatcc ccttggatcg
     1561 ccggagggcg aggacggcat cgcggaccac cgcgagcggc gcgcgcgccc ccacctcgat
     1621 tcctagcacg tccgcgagtt gcccatactg gagcggcgcg gagagggtcc ccctccgcat
     1681 ctgaaatgag acgtcgagga ccacgaaccg gggcgtcggg cgcc
//
LOCUS       DASVKP010000128         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_374662, whole genome shotgun sequence.
ACCESSION   DASVKP010000128 DASVKP010000000
VERSION     DASVKP010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374662"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="VF362_02645"
     CDS             complement(<1..1528)
                     /locus_tag="VF362_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7589856.1"
                     /translation="MDAREKATLSQNISTMMDEAWTTYAERKLFSHTKPESPTTWIHI
                     TGPEMRDQVDAVAKGLIARGIKPGDCVGIMARTRVEWSILDFAIWAAGAVSVPVYDTS
                     SEEQVDWITSDSDTRVLVVETSEHLQLAKTVAKGESPVRDILCIDHGALAELTKAGVG
                     VPDAELVRRKSLAKQDDLATIVYTSGTTGRPKGVRLTHFNYICHVQGLQKQIPEVLFQ
                     EGASTVLFLTLAHSLARLVEVVLAASGVALGYCPDTTQLLPMLESYRPTLILGVPRVF
                     EKVYNGAEQKASAGGKGGIFRWAAAQSVAYSTALDDPSGPSKSLAFKHGIANRLVLSK
                     IYAALGGNASWAVSGSAPLGARMGHFFRGLGIVVLEGYGLTETNAASHVNRATLVKMG
                     SVGPPIPGIEVKIADDGEILMRGDSLFEGYHHNPEATAEAIQNGWFHSGDIGTEDKDG
                     YLTITGRKKELIVTAGGKNVAPAVLEDRLRAHPLVSQCVAVGDGKPFIGALVTLDEDA
                     L"
     gene            1611..>2157
                     /locus_tag="VF362_02650"
     CDS             1611..>2157
                     /locus_tag="VF362_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX7589857.1"
                     /translation="MYAVGVDIGGTKIAAGVVDDGGRIVAKARRDTSAGDADGINHAI
                     AEVVAEFAKEYEFDAIGLAAAGFASSDREHIVFGPNIAAWRDYPLAARVRELVERDVS
                     VVVENDGNAAGWGEYVFGAARGRSTMIMVTLGTGLGAGMVVDGHLVRGAYGFAAELGH
                     MRVVPDGHLCGCGHRGCWEQYA"
BASE COUNT          323 a          665 c          787 g          382 t
ORIGIN      
        1 gcagcgcgtc ctcatcgagg gtgacgaggg caccgatgaa cggcttcccg tcgcccacgg
       61 cgacgcactg gctgacgagc gggtgcgcgc ggaggcgatc ctcgagcacc gcgggggcga
      121 cgttcttgcc ccccgcggtc acgatcagtt ccttcttgcg cccggtgatg gtgaggtagc
      181 cgtccttgtc ctcggtgccg atgtcgcccg agtggaacca cccgttctgg atcgcctcgg
      241 cggtggcctc ggggttgtgg tggtagccct cgaagagcga gtctccccgc ataaggatct
      301 cgccgtcgtc ggcgatcttg acctcgatgc cgggaatcgg cggtccgacg gagcccatct
      361 tcaccagggt ggcgcggttc acatgcgagg cggcgttcgt ctcggtcagg ccgtagccct
      421 caagcacgac gatgccgagc ccccggaaga agtggcccat gcgcgcgccg agcggcgccg
      481 atccgctgac cgcccaggag gcgtttcccc cgagcgcggc gtagatcttg ctcaggacga
      541 gtctgttggc gatgccgtgc ttgaacgcca gggacttgga gggtccgctg gggtcgtcga
      601 gcgccgtcga gtaggcgacg gattgggccg ccgcccagcg gaagatcccg cccttgcccc
      661 cggccgaggc cttctgctcg gcgccgttgt acaccttctc gaacacgcgc gggacgccga
      721 ggatgagcgt gggcctgtag ctctccagca tgggaagcag ttgcgtggtg tcggggcagt
      781 agccgagggc gacgcccgag gcggcgagga ccacctcgac gagccgggcg agcgagtgcg
      841 cgagggtaag gaagagcacg gtgctcgcgc cctcctggaa gagcacctcg gggatctgct
      901 tctggagccc ctgaacgtgg cagatgtagt tgaagtgggt aaggcgcact cccttggggc
      961 gacccgtcgt tcccgaggtg tagacgatgg tcgcgaggtc gtcctgcttg gcgagcgact
     1021 tccgccggac aagttcggcg tccgggacgc cgactccagc cttggtgagc tcggcgaggg
     1081 cgccgtgatc gatacacagg atgtccctca caggggattc gcccttggcg accgtcttgg
     1141 cgagctggag gtgttcgctc gtctcgacga cgaggacccg ggtgtcggag tctgaggtga
     1201 tccaatcgac ctgctcctca gaggaggtgt cgtagaccgg cacggacacg gctccggcgg
     1261 cccagatcgc gaagtcgagg atggaccact cgacccgcgt ccgcgccatg attcccacgc
     1321 aatcaccggg cttgatgccg cgggcgatga ggcccttggc gacggcgtct acctggtctc
     1381 gcatttccgg gcccgtgatg tggatccacg tggtggggct ctctggcttg gtgtgggaga
     1441 agagcttgcg ctccgcgtag gtcgtccaag cctcgtccat cattgtgctg atgttctgcg
     1501 agagtgttgc cttctcacgc gcgtccatgc tgcctccatt ggcatccgcc gggttggacg
     1561 ccacgatact ctgtgggggc tcgttacggt ctcgatggaa gggccaagaa atgtacgcgg
     1621 tcggtgtcga tatcggcggt accaagatcg ccgctggcgt cgtcgacgac ggcggtcgca
     1681 tcgtcgcgaa ggcgcggcgg gacacgtccg caggtgacgc ggacggcatc aaccacgcca
     1741 tcgcggaggt cgttgcggag ttcgccaagg agtacgagtt tgatgcgatt ggcctcgccg
     1801 cggcgggctt cgcgagctcc gatcgcgagc acatcgtgtt cgggcccaac atcgccgcgt
     1861 ggcgggacta cccgctcgcc gcgagggttc gcgaactcgt cgaacgcgac gtgagcgtcg
     1921 tcgtcgagaa cgacggcaac gccgcgggat ggggcgagta cgtcttcggc gccgcgcgcg
     1981 ggaggtcgac gatgatcatg gtcacgctcg gcaccgggct cggggcgggg atggtggtgg
     2041 acggccacct tgtgcgcggg gcgtacgggt tcgccgccga gttgggccac atgagggtcg
     2101 tacccgatgg gcacctctgc gggtgcggac atcgcggttg ctgggagcaa tacgcgt
//
LOCUS       DASVKP010000129         1515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_952762, whole genome shotgun sequence.
ACCESSION   DASVKP010000129 DASVKP010000000
VERSION     DASVKP010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952762"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VF362_02655"
     CDS             complement(<1..388)
                     /locus_tag="VF362_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX7589858.1"
                     /translation="MNSAIRRLSVIVLVMFLALMGSATWVQFFDANSLNADPRNRHTL
                     YREYGTFRGPIVVDGQAIASSSPVNDPFGYQRTYSDGWLYAPVTGFYSVVYGRTGIEQ
                     AENTVLNGSDDSLFWARLGDLLAGKEQ"
     gene            complement(385..>1515)
                     /locus_tag="VF362_02660"
     CDS             complement(385..>1515)
                     /locus_tag="VF362_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX7589859.1"
                     /translation="PVLFPAAFALNGVGLAMIYRLDLERAGTSTASHYATRQLAWSVI
                     GVTLAVLVIVALRDHRMLRRFTYTAMVAGLVFLLLPLLPVLGQERRGARLWIEVFGMS
                     FQPAEIAKILLAIFFAGYLVTHRDNLALAGPKVLGVRLPRLRDLGPIVLAWVASIAVL
                     VYEKDLGTSMLFFGLFVAMLYLATERLSWVLLGVGLFGAGAVAAGAIFPHVAARYDVW
                     LHALDPAVYNRSPGGSEQLVRGLFGLANGGLFGTGFGEGLPGQVPFAESDFIVSSLGE
                     ELGLTGLMALLALYLLIVERAFRTAIGMRDGFGKLLAAGLGFAIALQLFVVVGGVTRL
                     IPLTGLTTPFLAYGGSSLLANWIVIGLLLRMSHDSRREEALP"
BASE COUNT          309 a          472 c          536 g          198 t
ORIGIN      
        1 gctgctcctt gccggcgagc agatccccga gccgagccca gaagagcgag tcgtcggacc
       61 cgttgaggac cgtgttctcc gcctgttcga ttccggtgcg tccgtagacg acggagtaga
      121 agcccgtgac cggcgcgtac agccacccgt ccgagtaggt gcgttggtag ccgaacgggt
      181 cgttgacggg ggacgacgac gcgatggcct ggccatccac cacgatcggg cctcgaaacg
      241 tcccgtactc gcgatagagg gtgtggcggt tgcgtggatc ggcgttcagc gagttggcgt
      301 cgaagaactg cacccacgtc gcggagccca tgagggcgag gaacatcacc aggacgatga
      361 cgctgaggcg gcggatcgcg ctgttcacgg caacgcctct tcccgccggg agtcatggga
      421 catgcgcagg agcaacccaa tgacgatcca gttggccaag agagagctcc ccccgtaggc
      481 gaggaagggc gtcgtcagtc ccgtgagcgg gatcagacgg gtgacgcccc cgacgacgac
      541 gaacagttgc agggcgatgg cgaaccccag ccccgccgcg agaagtttgc cgaacccgtc
      601 gcgcatcccg atcgcggtgc ggaacgcgcg ttcgacgatg aggagataga gggcgagtag
      661 cgccatcagg ccggtgagcc ccaactcctc gccgagggag gagacgatga agtcggactc
      721 ggcaaacggc acctgccctg gaagcccctc gccaaagccg gttccgaaga gcccgccgtt
      781 ggcgaggccg aatagccccc tgaccagttg ctccgagccg ccggggctcc tgttgtacac
      841 ggcgggatcg agcgcgtgga gccacacgtc gtatcgcgcg gcgacatggg ggaagatcgc
      901 cccggcggca acggcgccag cgccgaacag gcctacgccg agaagcaccc agctcagccg
      961 ctcggtcgcg aggtagagca tggcgacgaa gagcccgaag aagagcatcg acgttccgag
     1021 gtccttctca tagacgagca ccgcgatcga cgcgacccag gcgaggacga taggccccag
     1081 atcgcgcagg cggggaaggc ggactccgag caccttgggc cccgcgagcg cgaggttgtc
     1141 ccgatgggtg acgaggtagc cggcaaagaa gatcgcgagg aggatcttcg cgatctcagc
     1201 gggctggaac gacatcccga acacctcgat ccagagccga gcgccgcggc gctcttgccc
     1261 gagaaccggc agcaggggaa gcaagaggaa gacgagtccg gcgaccatgg ccgtgtaggt
     1321 gaagcgtcgg agcatgcgat ggtcgcgcag ggcgacgatc acgagtaccg cgagggtgac
     1381 tccgatgacg ctccaggcga gttgtcgcgt cgcatagtgc gacgcagtcg aggttcccgc
     1441 ccgctcaaga tcgaggcggt agatcatggc caaccccacg ccgttgaggg cgaatgccgc
     1501 ggggaacaga accgg
//
LOCUS       DASVKP010000130         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_540188, whole genome shotgun sequence.
ACCESSION   DASVKP010000130 DASVKP010000000
VERSION     DASVKP010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540188"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VF362_02665"
     CDS             complement(<1..122)
                     /locus_tag="VF362_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013399112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX7589860.1"
                     /translation="MTRLVVLAGPTAVGKGTIVRRLVECYGDVWVSVSATTREPR"
     gene            complement(119..436)
                     /gene="mihF"
                     /locus_tag="VF362_02670"
     CDS             complement(119..436)
                     /gene="mihF"
                     /locus_tag="VF362_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX7589861.1"
                     /translation="MGTAPNLTSDERAAALRKATAVRTARREFKDAVAKRAFTLAGAI
                     EAAKADEGLTGIRVHDLLQCLPGVGPRRASARMGAAGIAASRRIRGLGQHQVDALVKL
                     DRS"
     gene            complement(441..1289)
                     /gene="pyrF"
                     /locus_tag="VF362_02675"
     CDS             complement(441..1289)
                     /gene="pyrF"
                     /locus_tag="VF362_02675"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX7589862.1"
                     /translation="MAFGAQLAAAMDKFGPLSVGIDPSPQMLAHWGLPDTAEGLLTFG
                     QAVVEASERSVAAVKIQIAFFERHGSRGIAALEQVLAFARHHSLITIMDAKRGDIDST
                     MAGYADAYLRPGSPLEVDAMTLSPYLGFDSLAPALDIAAEHGKGVFVLALTSNPEGAS
                     VQHSRNEDGVAVAAMIAAAAGARNRGESPMGSVGLVVGATVGDAIRALAVPLSSLNGP
                     ILAPGVGAQGAGSEQLAQVFQGVTRNVLSHQSRAVLMAGPFVEKMRGAIQAAAGAAQF
                     ALRGDE"
     gene            complement(1337..>1921)
                     /gene="carB"
                     /locus_tag="VF362_02680"
     CDS             complement(1337..>1921)
                     /gene="carB"
                     /locus_tag="VF362_02680"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882533.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEX7589863.1"
                     /translation="PFKRFRTAGGTVVDTVLGPEMRSTGEVMGIDVNFPTAFAKSQSA
                     AFGGLPTGGTVFISVADRDKRAMVLPVARLAQLGFEVLATEGTAQVLARNGIPARVVR
                     KYSEGPGPEGEGTIVDLIAAGGVDMVVNTPSGQGARADGYEIRAATTAADKAIVTTVQ
                     QLAAAVQGIEAVRAGPFTVTSLQDYHLQAQHLRD"
BASE COUNT          277 a          699 c          648 g          297 t
ORIGIN      
        1 cgcggctccc gtgtcgtcgc cgagacggac acccagacgt ccccgtagca ctccacgagg
       61 cgacggacga tcgttccctt gccgaccgcc gtcggacccg caaggaccac aagtcgcgtc
      121 atgagcgatc caacttgacg agcgcgtcga cctggtgctg ccccaggccc ctgatgcgcc
      181 gggaggccgc gatgcccgcc gcccccatcc tcgccgaggc gcgcctgggc ccgactccgg
      241 gaaggcactg gagcaggtcg tggacacgga ttccggtgag gccctcgtcc gccttggccg
      301 cctcgatggc acccgcgagc gtgaaggcgc gcttcgctac ggcgtccttg aactcgcgcc
      361 gggccgtccg caccgcggtc gccttcctca gcgccgccgc acgctcgtcg ctcgtcaggt
      421 tcggcgcggt acccattcgt ctactcgtcg cccctgagcg cgaactgggc cgccccggcg
      481 gccgcctgga tcgcgccacg catcttctcg acgaacgggc ccgccatcag gaccgcgcgg
      541 ctctggtggg acaggacgtt gcgcgtcacg ccctggaaca cttgcgcgag ctgctccgac
      601 cccgccccct gggctccgac gcccggggcg aggatgggcc cattcagcga gctcaggggc
      661 acggccaggg cgcggatcgc gtcgccgacc gtcgcgccaa cgacgaggcc cacgctcccc
      721 atggggcttt ccccccggtt gcgcgcaccg gccgcggcgg cgatcatggc cgcgacggcc
      781 accccgtcct cgttccggga gtgctgaacg ctcgcgccct cggggttcga ggtgagcgcg
      841 aggacgaaga cgcccttgcc gtgctcggcc gcgatgtcca gcgccggcgc gagggaatcg
      901 aaccccagat agggcgagag cgtcatggcg tcgacctcga ggggcgaacc cggacggagg
      961 taggcgtccg cgtaccccgc catcgtggag tcgatgtcgc cgcgcttcgc gtccatgatc
     1021 gtgatgagac tgtggtgccg cgcgaaggcc agcacctgct ccagcgcggc aatgccgcgg
     1081 gagccgtggc gctcgaagaa ggcgatctgg atcttgaccg cggcgaccga gcgctcggag
     1141 gcctcgacga cggcctgacc gaacgtcagc agtccctccg ccgtgtccgg caacccccag
     1201 tgagccagca tctgaggact cgggtcgatc cccaccgaga gcggcccgaa cttatccatc
     1261 gcggcggcca gttgagcccc aaacgccatg ttcgtctcct ctatcggtgg tcctgagggt
     1321 ggtggtcctg aatgatctag tcccgcaggt gctgcgcctg aaggtggtag tcctgcaggc
     1381 tcgtcacggt gaacgggcct gctcggaccg cctcaatgcc ttgcacggcg gcggcaagtt
     1441 gctggacggt ggtgacgatg gccttgtcgg ccgccgtcgt cgccgccctg atctcgtatc
     1501 cgtccgcgcg ggcgccctgt cccgaagggg tgttcaccac catgtcgacg ccgccagcgg
     1561 cgatcaggtc gacgatggtt ccctctccct cggggccggg cccctcagag tattttcgca
     1621 ccactctggc tgggatgcca tttcgcgcca gaacctgggc cgtcccctcg gtcgcgagca
     1681 cctcgaatcc gagctgcgcg agtcgcgcga cgggcaggac catcgcccgc ttgtcgcggt
     1741 cggcgacgga gatgaagacc gtgcctcccg tgggtagccc gccgaaggcc gccgactgag
     1801 acttcgcgaa ggcggtcggg aagttgacgt cgatgcccat tacctcgccc gtcgagcgca
     1861 tctccggtcc aaggaccgtg tcaacgaccg tgccccccgc ggtgcggaag cgtttgaagg
     1921 g
//
LOCUS       DASVKP010000131         1974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049 k141_44648,
            whole genome shotgun sequence.
ACCESSION   DASVKP010000131 DASVKP010000000
VERSION     DASVKP010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44648"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VF362_02685"
     CDS             complement(<1..350)
                     /locus_tag="VF362_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589864.1"
                     /translation="MSIPTSAPKPAPPKVSSTRPPSPKVLAGKTHHPVKVPVVSDLDA
                     AELAAAASHGVVDGESVVVVDGDVRHVVGPAEGDEPLLAYARRYFEHIGALERFHGRL
                     ETSELSIRDIDGTLT"
     gene            complement(347..1132)
                     /locus_tag="VF362_02690"
     CDS             complement(347..1132)
                     /locus_tag="VF362_02690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7589865.1"
                     /translation="MATNKQARAQQKRRYEQFQAKIAARQARRQRRQRIIAIVAIVVV
                     LAAFGAFGMVSLQTNDSAAPTVNVTTSPSPTPTATPTATTASGLAPAPSLAEHRTWTA
                     TITTNKGVITATLDGAAAPQAVSSFIYLAQKGFFNNSSCHRVVTAGIYVVQCGSPDGT
                     GTGGPGFTFGPVENAPTDDQYPAGTLAMARSGNNAYSMGSQFFFVYRDSQIPSDTAGG
                     YTVFGTITSGLAILEAIGAAGTSDGTTDGAPVEPVLINEVSVE"
     gene            1194..>1974
                     /locus_tag="VF362_02695"
     CDS             1194..>1974
                     /locus_tag="VF362_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX7589866.1"
                     /translation="MSRVVPVSGFPEWAPEERVVERHVIETLRQVFALHGFAEIETRA
                     IEPLARLRGDSDAAKEIYALRRVLADADDADSGMGLHFDLTVPFARYVEEFSGKLAFP
                     FRRYQIQKVWRGERPQEGRFREFYQADIDIVARETLPAHLEVEVAAVVARALGRLPIP
                     PVRIHVNDRRLVEAFYRGVGIADVAGALRSVDKLAKIGRDGVAAELRGAGVPDTAVGA
                     CLDLAAIASPDASFADGVRALGARHGVASAALEEGIASLAAL"
BASE COUNT          280 a          645 c          728 g          321 t
ORIGIN      
        1 gtgagcgtcc cgtcaatgtc gcggatcgac agttcggacg tctcaaggcg cccgtggaag
       61 cgctcgagcg cccctatgtg ctcgaagtag cggcgcgcgt aggcgagcaa gggttcgtcg
      121 ccttcggcgg ggcccacgac gtgccgcacg tcgccgtcga ccaccacgac ggactcgccg
      181 tcgaccactc cgtgcgacgc cgcggccgcg agttcagcgg cgtcgagatc cgagaccacc
      241 ggcaccttca cggggtgatg ggtcttgccc gcgagcacct tcggcgaggg aggccgcgtc
      301 gacgaaacct tgggcggcgc gggcttcgga gcggacgtcg gaatgctcat tccacagaca
      361 cctcattgat aaggacgggc tcgacggggg ccccgtcggt ggtgccatca gacgtccctg
      421 cggcgccgat tgcttccagg atagccaggc cgctggtgat cgtgccaaag accgtgtacc
      481 cgccggcggt gtccgagggg atctgcgagt cccgatagac gaagaagaac tggctcccca
      541 tggagtacgc gttgttgccg ctccgcgcca tagcgagcgt tccggcgggg tactggtcgt
      601 ccgtcggggc gttctcgacg ggtccgaagg tgaagcccgg tccgccggtg cccgtgccgt
      661 cgggcgagcc gcactggaca acatagatgc ccgcggtgac cacgcggtgg caggagctgt
      721 tgttgaagaa ccccttctgc gcgaggtaga tgaaggacga gaccgcctgc ggggccgccg
      781 cgccgtcgag ggtcgcggtg atgacgccct tgttcgtcgt gatcgtcgcg gtccacgtgc
      841 ggtgctcggc cagtgacggg gcgggggcga ggcccgaggc cgtcgtggcc gtgggcgtcg
      901 ccgtgggcgt gggcgagggc gaggtcgtga cgttgacggt gggtgccgcg gaatcgttcg
      961 tctggaggga caccatgccg aaggcgccga aggccgcaag gacgaccacg atcgccacga
     1021 tggcgatgat gcgctgtcgg cgttgacgac gcgcctggcg ggccgcgatc ttcgcctgga
     1081 actgctcgta gcgccgcttc tgttgggcgc gggcctgctt gttcgtcgcc atggtccctc
     1141 cgcgggggtg tcgagtcgcc agtctagggg cttggagatg gaacaatggc cgcatgagcc
     1201 gcgtcgtgcc cgtctccggg ttcccagaat gggcgcccga agaacgggtc gtcgagcgcc
     1261 acgtcatcga aacgctgagg caggtcttcg cgctccatgg attcgcggag atcgagacgc
     1321 gtgcgatcga accgctcgcc cggttgcgcg gcgactcgga cgccgcgaag gagatctacg
     1381 cgcttcgccg agtcctcgcg gatgcggatg acgcggattc cggcatggga ctccacttcg
     1441 atctcacggt tccgttcgcg cgctacgtcg aggagttctc aggcaagctc gcgttcccgt
     1501 tccgccgcta ccagatccaa aaggtgtggc ggggcgagcg cccgcaggag ggacgcttcc
     1561 gcgagttcta ccaggccgac atagacatcg tcgcgcgcga gacccttccc gcccaccttg
     1621 aggtcgaggt cgcggcggtc gtggcccgcg ccctgggacg gctcccgatc cctcccgtgc
     1681 gcatccacgt caatgaccgt cgcctggtcg aggccttcta ccgcggcgtg gggatcgccg
     1741 acgtcgccgg cgcgctgagg tccgtggaca agctcgcgaa gatcgggagg gacggcgtcg
     1801 cggccgagtt gcgcggggcg ggggtgcccg acaccgccgt gggcgcgtgc ctcgacctcg
     1861 ccgccatcgc ctcgcccgat gcgtcgttcg ccgatggggt tcgcgcactc ggggcgcggc
     1921 acggcgtcgc gtcggccgcg ctcgaggagg gcatcgcgtc cctcgccgcg ctcg
//
LOCUS       DASVKP010000132         4007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_624153, whole genome shotgun sequence.
ACCESSION   DASVKP010000132 DASVKP010000000
VERSION     DASVKP010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624153"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /gene="recA"
                     /locus_tag="VF362_02700"
     CDS             <1..1166
                     /gene="recA"
                     /locus_tag="VF362_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX7589867.1"
                     /translation="LGEGQRVAVEIIPSGSIALDVALGIGGLPRGRVVEIYGPESSGK
                     TTLALHAVANVQKAGGVAAFIDAEHALDPEYASKLGVDVEQLLISQPDTGEQALEIAD
                     ILIRSGGIDVLVIDSVAALVPKAEIEGEMGDSHVGLQARLMSQALRKITGGLSNSGTT
                     AIFINQLREKIGVFFGSPETTTGGKALKFYASIRLDIRRIETLKEGTDAVGNRTRVKV
                     VKNKMAPPFKQAEFDILYGHGISREGEIIDLGVEHGFIRKSGAWYTCEGDQLGQGKEN
                     ARAFLMDNPRLSDELEIKIKQKLGVGPGAGAAGEESQVAGMSAEDAMSAEDVVAAEDV
                     VAIEAIGVEAIGEVDEASRMLAAADGDVAGAFVNSSVVNSPVIENSVLDSAVQ"
     gene            1170..1718
                     /locus_tag="VF362_02705"
     CDS             1170..1718
                     /locus_tag="VF362_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX7589868.1"
                     /translation="MTGRSPGLSGVPAPDEAERARQVALRILDAAPRSAGQLREALLA
                     REVAEAVADGVIERYREVGLIDDGGLARMIARTRHGERGQSRRAIAQELRRKGFVADD
                     IATALEEISDEDESAAAEALAAKRWEALNDIPREARVRRVVALLGRKGYPPGVAFDLV
                     RRLERADIWEDREVSPAEEEVT"
     gene            1715..3184
                     /gene="rny"
                     /locus_tag="VF362_02710"
     CDS             1715..3184
                     /gene="rny"
                     /locus_tag="VF362_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX7589869.1"
                     /translation="MITVDQIVQIVLVLGLTLVALYVVILGRRDAEKERESAAREASA
                     LRDEAKSLHAEAQRREERIARREHEVAEDQRNLGTYQRTLDDRAATLAVTERQLEAER
                     AALRERQDAALAAVAGLDVAEARKRVTERLTAEAEADAAGAIRRIEKRVEAEAATRAR
                     EILVTAMQRQAVESSAQQSVTRVELPSEEMKGRLIGREGRNIRTFESVTGVSMILEDG
                     VDSVLLSSFDVERREIAEVTLRALVSDGRVQPQRIEAAHAAAVESAPARASTAGVDAA
                     ESAKVGGLSPIMIETLGRLRLRTSYGQNVLAHVVECAGLAAAIAEEVGGDPETARRAA
                     FLHDIGKAFVGEREGTHAALGAALALENGESAAVANAIAAHHDEVALESLEAVVVQIA
                     DALSASRPGARREDFDGYVERMSSLETMVREMPGVCDVVAMHAGREVRVVVAPELVGD
                     AALPELARTIARRINASPKYPGEVKVTVIRELRAEAVAG"
     gene            3189..>4007
                     /locus_tag="VF362_02715"
     CDS             3189..>4007
                     /locus_tag="VF362_02715"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HEX7589870.1"
                     /translation="MTDTSTARTYLVRTLGCQMNVHDSEHMAGMLERAGYERAPEGAA
                     GADVVVLNTCAVRENAATRLYGNLGALASEKARRPGMQIAVGGCLAQKDRGAILDAAP
                     WVDVVFGTHNMDVLPVLLARARHNNEAQVEIEESLTVFPSTLPSRRESLASAWVSISV
                     GCNNTCTFCIVPSLRGRERDRRPDEILAEVEALVSEGAIEVTLLGQNVNTYGVEFGDK
                     GAFASLLRACGEIQGLERVRFTSPHPAAFTEDVILAMAETENVMPSLHMPLQSGS"
BASE COUNT          677 a         1286 c         1448 g          596 t
ORIGIN      
        1 gcctgggcga ggggcagcgg gtcgccgtcg agatcatccc gagcggatcg atcgccctcg
       61 acgtcgcgct cggcatcggg ggactcccgc gcggcagggt cgtggagatc tacggtccgg
      121 agtcgagcgg aaagacgacg ctcgcgctcc acgcggtcgc gaacgtccag aaggcgggcg
      181 gcgtcgcggc cttcattgac gcggaacacg cgctcgatcc ggagtatgcc agcaaactcg
      241 gcgtcgatgt cgagcagttg ctgatctctc agccggacac gggcgagcag gcgctcgaga
      301 tcgcggacat cctgatccgc tcgggcggca tcgatgtgct cgtgatcgac tcggtcgcgg
      361 ccctggtccc caaggcggaa atcgagggag agatgggcga cagtcacgtc gggctccagg
      421 cccggctcat gtcccaagcg ctgcgcaaga tcacgggcgg gctgagcaac tccggcacga
      481 ccgcgatctt catcaaccaa ctccgcgaga agatcggcgt gttcttcggg tcgcccgaaa
      541 ccactaccgg cggcaaggcc ctcaagttct acgcctcgat tcgcctcgac atccgccgca
      601 tcgagaccct caaggagggc accgatgcag tgggaaaccg aacccgcgtc aaggtcgtca
      661 agaacaagat ggcacccccg ttcaagcagg cggagtttga catcctctac ggccacggga
      721 tttcccgtga gggcgagatc atcgacctcg gcgtggagca cggcttcatc cgcaagtcgg
      781 gcgcctggta cacatgcgag ggcgaccaac tcggtcaggg caaggagaac gctcgggcgt
      841 tccttatgga taatccgcgt ctgagcgacg agctcgagat caagatcaag cagaagctcg
      901 gcgtgggtcc cggtgccggc gccgcgggtg aggagagcca ggtcgccggg atgtccgcgg
      961 aggacgcgat gtccgcggaa gacgtggttg ccgcggagga tgtggtcgcg atcgaggcca
     1021 tcggcgttga ggcaatcggc gaggtcgacg aggcgtcgcg gatgctcgcg gcggccgatg
     1081 gcgacgtcgc gggcgccttc gtcaatagtt ccgtcgtcaa cagccccgtc atcgagaact
     1141 ccgttctcga cagcgccgtt cagtaggccg tgacggggag gagcccgggc ctctccggcg
     1201 tcccggcgcc ggatgaggcc gagcgcgcca ggcaggtcgc gctcaggatt ctcgacgctg
     1261 cgcccagaag cgcgggccaa ctccgcgagg cgcttctcgc gagggaagtc gccgaggccg
     1321 ttgcggacgg ggtcatcgag cgctaccgcg aggtgggcct catcgacgat gggggcctcg
     1381 cgcggatgat tgcgcgcacg cgccacggtg aacgcggcca gtcccggcgc gcgatcgccc
     1441 aggagctccg gcgaaaggga ttcgtcgccg acgacattgc gaccgcgctt gaggagatct
     1501 cggacgagga cgagtcagcg gccgcggagg cgcttgcggc gaagcgctgg gaggccctga
     1561 acgatatccc gcgcgaggcc cgggtgcgga gggtcgtggc gctgctcggg cgcaagggct
     1621 acccgcctgg ggtcgccttt gaccttgtga ggcggctcga acgtgccgat atctgggagg
     1681 atcgggaggt gagccccgcc gaggaggaag ttacgtgatc accgtcgacc aaatcgtcca
     1741 gatcgttctg gtgcttggtt tgacgttggt tgccctttac gtcgtgatcc tcggaaggcg
     1801 cgacgcggag aaggagcggg agtcggcggc gcgagaggcg tcggcgctcc gcgatgaagc
     1861 gaagtccctt cacgcggaag cgcagcggcg cgaggagaga atcgctcgcc gggaacacga
     1921 ggttgccgag gatcagcgaa acctcggaac ctaccagcgg accctcgatg accgtgcagc
     1981 gacgctcgcc gtgacggagc gccaactcga ggccgagcgc gcggcgcttc gcgagaggca
     2041 agacgccgcg ctcgctgcgg tcgcgggcct ggacgtcgcg gaggcgagga agagagtcac
     2101 cgaacgactc acggccgagg cggaggccga cgccgcggga gccatccggc gcatcgagaa
     2161 gcgagtggag gccgaggccg cgacccgcgc ccgggagatc ctggtgaccg ccatgcagcg
     2221 acaggccgtc gaatcctcgg cgcagcagag cgtcacgcgc gtcgagcttc cgtcggagga
     2281 gatgaagggc aggctcatcg gtcgcgaggg caggaacatc cgcaccttcg agtcggtgac
     2341 cggggtgtcg atgattcttg aggacggcgt cgattccgtg ctcctctctt ccttcgacgt
     2401 cgaacgcagg gagattgccg aggtgaccct ccgcgccctc gtctccgacg gtcgggttca
     2461 gccccagaga attgaagcgg cccacgcggc cgccgtagag tccgcgcccg cgcgggcctc
     2521 gaccgccggg gttgacgccg cagagtcggc caaggtcggc ggtctctcgc cgatcatgat
     2581 cgagaccctc ggcaggcttc gcctgcgcac gtcgtacggc cagaacgtgc tcgcccacgt
     2641 tgtcgagtgc gccggactcg cggcggcgat tgccgaggaa gtcggtggcg atcccgagac
     2701 ggcgcgccgc gccgcgttcc tgcacgacat tggaaaggcc ttcgtgggcg aacgagaggg
     2761 cacgcacgcc gccctcggcg cggccctcgc cctggagaat ggggagtccg ccgcggtcgc
     2821 gaacgcgatc gcggcgcacc atgacgaggt cgccctggag tcgctcgagg cggtagtcgt
     2881 tcagatcgct gacgcgcttt cggcgtcgcg gcccggtgcc cgccgcgagg acttcgacgg
     2941 ctacgtcgaa cggatgtcgt cgctcgagac catggtgcgc gagatgccgg gggtatgcga
     3001 cgtcgtcgcg atgcacgccg ggcgcgaggt acgcgtcgtc gtcgcaccgg aactcgtggg
     3061 ggacgccgcg ctgcccgaac tggcacgtac gatagccaga cgcatcaacg caagcccgaa
     3121 gtaccccggg gaggtcaagg tcaccgtcat ccgcgagttg cgtgcggagg ctgtagccgg
     3181 atagtgcgat gaccgacaca tctacggctc ggacgtatct cgtgcggacc ctcgggtgcc
     3241 agatgaatgt tcacgactcc gaacacatgg ctggcatgct cgagcgcgcg ggctacgagc
     3301 gcgcgccaga gggcgcggcc ggggccgacg tcgtcgtcct caacacgtgc gcggtgcgcg
     3361 aaaacgccgc gacccgtctc tacgggaacc tcggcgcgct ggcctcggag aaggcccgta
     3421 ggccggggat gcagatcgcg gtggggggtt gcctcgccca gaaggatcgc ggcgcgattc
     3481 tcgacgccgc gccgtgggtc gacgtggtgt tcgggacgca caacatggac gtgctccccg
     3541 ttctcctcgc gcgggctcgc cacaacaatg aggctcaggt ggagatcgag gagagcctca
     3601 ccgtcttccc gtcgaccctg ccgtcgcgcc gggagtccct tgcgtccgcc tgggtgtcga
     3661 tctccgtggg ctgcaacaac acgtgcacgt tctgcatcgt gccgtcgctc cggggcaggg
     3721 agcgcgatcg ccgccccgac gagatcctcg ctgaggtcga ggccctcgtt tctgagggag
     3781 ccatcgaggt cacgctgctc gggcagaacg tgaacacgta cggggtcgag ttcggcgaca
     3841 agggggcttt cgcgagcctc ctgcgcgcct gcggagagat ccaggggctg gagcgcgtcc
     3901 ggttcacgtc gccccacccc gcggcattca ccgaggacgt catcctcgct atggccgaga
     3961 ccgaaaacgt catgccgagc ctgcacatgc ccctgcagtc gggctcg
//
LOCUS       DASVKP010000133         1976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_211217, whole genome shotgun sequence.
ACCESSION   DASVKP010000133 DASVKP010000000
VERSION     DASVKP010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211217"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VF362_02720"
     CDS             <1..530
                     /locus_tag="VF362_02720"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX7589871.1"
                     /translation="VVAMAETMNVELVLNPDNPLVLLDDEEVSVLLRSEAVTGAVSAV
                     STNLEARAALGRWQRELIAREVKGGFSEGRGVVAEGRDITTVIAPDADVRVLMTAHPE
                     VRVARRAAEGGHEAGVHASIAQIRAGVLGRDAKDSTVVNFTTAADGVVTLDTTDLTID
                     EAVWAIVELAREARP"
     gene            527..1252
                     /locus_tag="VF362_02725"
     CDS             527..1252
                     /locus_tag="VF362_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7589872.1"
                     /translation="MTREAMPPTHPKPLGRLKAPSRLGPVWGRPIAWLLTHAFWRTDV
                     RGKQHVPRKGPVVVIANHVGFIDGPVATGVTPRYMHVLVKGEMFTNVLGPILRGSGQI
                     EVWHGGREALARGRAVLQRGGVLGVFPEGTRGMGDAASVGGGAAWLAIHGGAPVVPLA
                     LLGTRLTGESVNVWPRPGRRLIAEFGEPIRLDLPEAAHGRERQQMAEQIIADALRAHV
                     RRVAAGAPIGLPQDDPLREGPLA"
     gene            1249..>1976
                     /locus_tag="VF362_02730"
     CDS             1249..>1976
                     /locus_tag="VF362_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX7589873.1"
                     /translation="MTNSTGDDILGPGELPLAAPHDSDAPEDADAVREAALRAGLEAY
                     DLTDEDLTELSEEPQREQAILPILAVVGRPNVGKSTLVNRIIGQRAAVVEDTPGITRD
                     RVTYPGEWAGHEFILMDTGGWETKVAGLDLSVARQAEVAIDMADAIIFVVDATVGPTA
                     TDEQVVKLLRRANKPVVLCANKVDGPRGESMATELWALGLGEPYAVSALHGRGMGDFL
                     DATVALLPTELAREAVAKDAPRRVA"
BASE COUNT          291 a          691 c          732 g          262 t
ORIGIN      
        1 atgtggtcgc gatggccgag acgatgaacg tggagttggt gctcaatccc gacaacccgc
       61 tcgtcctgct cgatgacgag gaggtctcgg tgctcctgcg ctccgaggcc gtgacgggcg
      121 cggtgtcggc ggtctccacc aacctcgagg cgcgggccgc gctcggccgc tggcagcgcg
      181 agctgatcgc gagggaggtc aagggcggct tctccgaggg caggggcgtc gtcgccgagg
      241 ggcgcgacat caccacagtc atcgcgcccg acgccgacgt gcgggtgctg atgacggctc
      301 atccggaagt gcgggttgcc cgccgggccg ccgagggcgg gcacgaggcc ggggtccatg
      361 cgtccatcgc gcagattcgc gcgggcgttc tgggccgcga cgccaaggat tcgacggtgg
      421 tcaacttcac cacggcggcc gacggcgtcg tcaccctgga cacgacggac ctgaccatcg
      481 acgaggccgt ctgggccatc gtcgagctgg cgcgggaggc gcggccgtga cccgcgaggc
      541 catgccgccg acccacccca agccgctcgg tcgcctgaag gccccgagcc gcctcggccc
      601 cgtgtgggga cgccccatcg cgtggctact gacgcacgcc ttctggcgca cggacgttcg
      661 gggcaagcag cacgtgcccc gcaaggggcc cgtcgtggtg attgcgaacc acgtcgggtt
      721 catcgacggg cccgtcgcaa cgggcgtgac gccccgctac atgcacgtcc tggtgaaggg
      781 ggagatgttc accaacgtgc tcggccccat cctgcgaggc tccgggcaga tcgaggtgtg
      841 gcacgggggc cgcgaggccc tcgcccgcgg ccgcgcggtc cttcagcgcg gcggggtcct
      901 gggagtattc cccgagggca cgcgcggcat gggcgatgcc gcgagcgtcg gcgggggagc
      961 agcgtggctc gccatccacg gcggcgcgcc cgtcgtcccg cttgccctgc tcgggacccg
     1021 cctgacggga gagagcgtca acgtctggcc gcggcccggc aggcggctca tcgccgagtt
     1081 cggcgagccg attcggctcg atctgcccga ggcggcgcac ggccgcgaga ggcagcagat
     1141 ggcggagcag atcattgccg acgcgcttcg cgcccacgtt cgccgtgtcg cggcgggcgc
     1201 acccatcggg cttccgcagg acgacccgct ccgtgaaggg cccctcgcat gacgaacagc
     1261 accggcgacg acatcctcgg cccgggcgaa cttccgctcg ccgcccccca cgactcggac
     1321 gccccggagg acgccgacgc agtgcgcgag gccgccctgc gggcggggct cgaggcgtac
     1381 gacctcacgg acgaggacct gaccgaactc agcgaggagc ctcagcgcga gcaggcgatc
     1441 cttccgattc tggccgtggt tgggcgcccg aacgtcggca agtccacgct cgtcaaccgc
     1501 atcatcggcc agcgcgccgc cgtagtggag gacaccccgg ggatcactcg cgaccgcgtc
     1561 acgtacccgg gcgagtgggc cggccacgag ttcatcctga tggacacggg cggatgggag
     1621 accaaggtcg cggggctcga cctcagcgtc gcgcgccagg ccgaggtcgc gatcgacatg
     1681 gccgacgcga tcatcttcgt cgtcgacgct acggtagggc cgacggcgac ggacgagcag
     1741 gtggtcaagt tgctccgacg cgcgaacaag cccgtcgtcc tgtgcgcgaa caaggtggat
     1801 ggaccgcgcg gcgagtccat ggcgaccgaa ctgtgggcgc tcgggctcgg cgagccgtac
     1861 gccgtgagcg cgctgcatgg acgaggcatg ggcgacttcc tcgatgcgac ggtcgctctc
     1921 ctgcccacgg agttggccag ggaggccgtc gccaaggacg ccccgaggcg cgtcgc
//
LOCUS       DASVKP010000134         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1605928, whole genome shotgun sequence.
ACCESSION   DASVKP010000134 DASVKP010000000
VERSION     DASVKP010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605928"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1950)
                     /locus_tag="VF362_02735"
     CDS             complement(<1..1950)
                     /locus_tag="VF362_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589874.1"
                     /translation="MGTDREGWIAPDGSVFLNVDWTRTNSFGGPQAPPIFPSARSVNI
                     WKDGSFREIVTSTEFGWRLLAVPKRDGSGGWVVRQDENGTALLSASNDGAVTGAWNDP
                     AFPHVEAIYASNSGTSLLLHTSIRHEMSNALGLGIWTVGDARPPSYDEIVLNEQTDPS
                     LLHVDVDRAADGAEILFDSRTSFEEPPGPSCCWRQPVFSRPTLLRTSLRQRLVVPAAG
                     RGPGANGSEWRTDLVLRNEGAAVSSVALRLLGNASTTPAVADASVDIGPGRILVIDDA
                     LMTLFGLERGSGALLLTPGAGTSLRATSRTYTRTSAGTFGMAVDAEDIVTAGRPGNLD
                     TFAAAFVGNGFRTNLISTDLSGGGSTLSASPTVNGEAFGPYLVSSPPGAQTQIDDVAR
                     FVPSPAGETGSIATTCVSGAPVTGFTVIDDMTNDPVWWGPDLPAGDLMIPAAVHAGGA
                     HGASFRTDLYVYNPGAEERGVSLAAQPWNDKSGLVWKNVTLAPGEARRIPDVLASLFG
                     LSGVATLSTTTALFDGTISMTSRTYAAMPDGSTYGMSVPPLQRSDAGDPFNAVEILGP
                     VGGPSLRTNLACITAFGVSASVRISIFDESGTKLDTFDQLLPDNGGVQINDLFRARGL
                     GDGPRAALIRVEGIPPDDPYASMSFY"
BASE COUNT          337 a          654 c          707 g          308 t
ORIGIN      
        1 gtagaaagac atcgaggcgt aggggtcgtc cggagggatg ccttcgaccc ggatgagcgc
       61 ggccctcggt ccgtctccga gcccgcgcgc gcggaagagg tcgttgatct ggacgccgcc
      121 gttgtccggc aggagctggt cgaacgtgtc gagcttcgtg ccgctttcgt cgaagatgga
      181 gatgcgcacc gaggcggaaa cgccgaatgc cgtgatgcac gcgagattcg ttcgaagcga
      241 cgggccgccc acgggtccga ggatctccac cgcgttgaaa ggatctcctg catcggaccg
      301 ctggagcggc ggcacgctca tgccgtacgt ggatccgtcg ggcatggcgg cgtatgtgcg
      361 cgatgtcatg gaaatcgttc cgtcgaagag ggccgtcgtc gtcgaaagag tcgcgacgcc
      421 cgagagaccg aacaaactcg ccagaacgtc ggggattcgc cgggcctcgc ccggcgcaag
      481 ggtcacgttc ttccagacca ggccggactt gtcgttccag ggctgtgcgg cgagggagac
      541 gccgcgctcc tcggcgcccg ggttgtacac gtagagatcg gtccggaacg aagcgccgtg
      601 cgcgccaccc gcgtggacgg cggccggaat catgaggtcg cccgcaggga gatcggggcc
      661 ccaccagacg gggtcgttcg tcatgtcgtc gatgaccgtg aatccggtga cgggggcccc
      721 ggacacgcac gtggtcgcga tggaaccggt ttctcccgcg ggcgacggta cgaaccgggc
      781 gacgtcgtcg atttgcgttt gcgctcccgg gggcgaggaa acgagatacg ggccgaacgc
      841 ctccccgttc accgttggac tcgccgacag cgtggagccc ccgccggaca gatccgtcga
      901 gatcaaattc gttcgaaagc cgtttccgac gaaagccgcg gcgaaggtat cgagattccc
      961 tggacggccc gcggtgacga tgtcctccgc atccaccgcc atgccgaagg tccccgcgct
     1021 ggtgcgcgtg tacgtgcggc tcgttgcacg gagcgacgtt cccgcccccg gcgtcaggag
     1081 cagggcgcct gagccgcgct ccaggccgaa aagcgtcatg agcgcgtcgt cgatgacgag
     1141 gatccggcct gggccgatgt cgaccgaggc atccgccacg gccggcgtcg tcgaggcgtt
     1201 cccgagaagt cgaagcgcca ccgacgacac tgctgcgcct tcattgcgca ggacgaggtc
     1261 cgttcgccac tccgagccgt tggcgccggg gccgcgacct gcggcgggga cgaccaggcg
     1321 ctggcggagc gaggtccgga gcagggtcgg cctggagaag accggctgcc gccaacaaca
     1381 agagggcccc ggcggctctt cgaaagatgt tcgcgagtcg aagaggatct cggcaccgtc
     1441 ggctgcgcga tcgacatcga cgtggaggag ggaggggtct gtctgctcgt tcagaacgat
     1501 ctcgtcgtag gagggaggcc gcgcgtcccc cacggtccag atgccgaggc ccagcgcgtt
     1561 cgacatctcg tgtcgaatcg atgtgtgcag aagcagcgac gtcccggaat tcgaggcgta
     1621 gatggcctcg acgtggggga aggcgggatc gttccacgcg cccgtcacgg cgccgtcatt
     1681 cgacgccgag aggagggccg ttccgttctc gtcctgcctc acgacccagc cgcccgaacc
     1741 gtcccgtttc ggaaccgcca gaagccgcca cccgaactcg gtcgaggtga cgatctcgcg
     1801 gaagcttcca tctttccaga tgttcacgga gcgcgccgac gggaagatcg ggggagcctg
     1861 cggtcccccg aacgagttgg tccgcgtcca gtcgacgttc aggaagacgg agccgtccgg
     1921 cgcgatccag ccctcgcggt ctgtccccac tccggcggga tcaccgcgag cgcctgtccg
     1981 cccgcgcctc tcagcgcgcg tagccc
//
LOCUS       DASVKP010000135         6919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_871601, whole genome shotgun sequence.
ACCESSION   DASVKP010000135 DASVKP010000000
VERSION     DASVKP010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6919
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871601"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VF362_02740"
     CDS             complement(<1..308)
                     /locus_tag="VF362_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HEX7589875.1"
                     /translation="MDDATAAVLAQIPPGTLIQRLGIEVTEVSAARAVGTMPIDGNTQ
                     PYGLLHGGATAALAETLGSLAAMVHAGPDGIAVGVDLNITHHRAARAGAVTGVATQIY
                     A"
     gene            328..2976
                     /gene="polA"
                     /locus_tag="VF362_02745"
     CDS             328..2976
                     /gene="polA"
                     /locus_tag="VF362_02745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX7589876.1"
                     /translation="MSESRQGSLLLIDGFSMAFRAFFALPADNFQTSTGVPVNAVYGF
                     ASMIVSLIADHKPTHMAVAFDLPGGTFRTVRYPPYKGTRGETPPEFVPQIPLLREVLA
                     ALRIPAIDKVNYEADDILATYARQGAEQGFEVFVVSGDRDTLQLVTDRVTVLYPRKGV
                     SDLAVMTPEAVREKYGVAPAKYPDLAALVGETSDNIPGVPGVGPKTAAKWVETYGGLD
                     QILAAAGDIAGKVGESLRENLEQVALNREINHLLTDLDLPLSIDDLVRVGGDAATIHA
                     LCDALQFRGLRPRLLALAADASDPAAPVDGSGDPAVVTEGAIEAIASLPGPVAMALVG
                     GKDPRALGVASPGGGVWAVDLTSLTPVEEQAIGAWLSSASVPKVLHGAKAAWHGLAAR
                     GYELAGIAGDTEIAAYLVQPDQRGFDLEDVMQRYLGVAVAGSKSDELDLGLGSSEAHV
                     AGLRAWTVSRLAEELEGALDSRKMTSLYRELEIPLIGVLAAMEAAGIAVDGAHLRMLA
                     GECSARAESAQREAWRAIGGFEVNLGSPKQLQEVLFDRLDMPKTKKIKTGYTTDAAAL
                     AELYEKRPHPFLEALLAYRAAAKLGQIIETLASAIGPDGRIHTTFSQVVASTGRLSSL
                     DPNLQNIPIRTDDGHRIREAFRVGEGYETLLTADYSQIEMRIMAHLSGDDQLIEAFRS
                     GEDLHRFVGGRVFGVQPENVTPEMRSKVKAMSYGLAYGLSSFGLARQLGTSTGEAQAL
                     MDDYFARFGEVRDYLASVVREARVTGYTETIFGRRRYLPDLNSTDRQRRDMAERMALN
                     APIQGSAADLIKRAMIGVSRRLEAESLASRQILQVHDELVVEVARGEKSAVEAILREE
                     MAGAADLSVPLDVHLGSGENWRIAGH"
     gene            3015..4037
                     /locus_tag="VF362_02750"
     CDS             3015..4037
                     /locus_tag="VF362_02750"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX7589877.1"
                     /translation="MRIGVLTGGGDCPGLNAAIRAIVKRGSSEHGHSIVGFRDGWRGF
                     LDGNSIPLDRNDVRGVLVHGGTMLGTARCHPDKEPGGLERVREVVEAERLDAIICIGG
                     DGTLKAASRVADTGIPVIGIPKTIDNDVVGTDVSIGFDTAMMIATEAIDRLHSTAESH
                     NRVMVVELMGRHSGWIAVAAGIAGGADIILAPEEPFDMEKVAKRITYRHRSQAGFSIV
                     AVAEGARPSEESDWSLDTTTDEAGNPIAGEIGQQVTREIQARTGYEARLTVLGYVQRG
                     GTPTAADRLLATRMGVAAIDAATLGVTKKFTAVRGPEIVLEDFSVMVGKTRFVPKELL
                     DAARGL"
     gene            4177..5658
                     /gene="rpsA"
                     /locus_tag="VF362_02755"
     CDS             4177..5658
                     /gene="rpsA"
                     /locus_tag="VF362_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX7589878.1"
                     /translation="MSPSPSKTPTVAVNDIGSAEDFLAAVDATIKNFDDGDLVTGVIV
                     KVDRDEVLLDIGYKTEGVIPSRELSIRHDADPDDLVKVGDTIEALVLTKEDKEGRLIL
                     SKKRAQYERAWGTIEKIKEEDGIVTGTVIEVVKGGLIMDIGLRGFLPASLVEMRRVRD
                     LGPYIGQELEAKIIELDKNRNNVVLSRRAFLEQTQSEVRSTFLTALAPGQVRKGVVSS
                     IVNFGAFVDLGGVDGLVHVSELSWKHIDHPGEVVTVGQEVDVEVLEIDMDRERVSLSL
                     KATQEDPWAVYARTHAIGQIVPGKVTKLVPFGAFVRVFEGIEGLVHISELSTRHVELP
                     EQVVSPEDAVFVKIIDIDLERRRISLSIKQANEGVDPEGEDFDPALYGMTAEYDAEGS
                     YKYPEGFDSATNEWRDGFDEQRELWEADYGRAHDRWVAHKAFVAKHEAVTAELEESSP
                     TPKKRRPAAGPSSYSSSEEGEEDAGTLASDERLAALREKLTGE"
     gene            5658..6260
                     /gene="coaE"
                     /locus_tag="VF362_02760"
     CDS             5658..6260
                     /gene="coaE"
                     /locus_tag="VF362_02760"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832859.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX7589879.1"
                     /translation="MLRIGLTGGIASGKSVASERFAQLGARVIDHDDLARQAVQPESA
                     ALADIAREFGDRLITRGKLDRAALADIVFGDPEARAKVNAIVHPYIFALAKAADSQAR
                     LDGVKVIVHAIPLLVETGLRGSWDTVVTIAAPAGVRTARLVKERGLTAAQAESRLSAQ
                     VTDDERARWADIVLDGGGTVAELRKQVDRFWKDHVPVSAR"
     gene            6275..>6919
                     /locus_tag="VF362_02765"
     CDS             6275..>6919
                     /locus_tag="VF362_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018135193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX7589880.1"
                     /translation="MTAPDLRRSEAPFRVISDYTPSGDQPTAIDELEARVRAGERDIV
                     LLGATGTGKSATTAWLIERLQRPTLVLAHNKTLAAQLANEFRELLPNNAVEYFVSYYD
                     YYQPEAYVPQTDTFIEKDSSINEEVERLRYSATNSLLTRRDVVVVSSVSCIYGLGTPE
                     EYIRGMATLEVGQQIERDTLLRKFVRMQYTRNDLAFTRGTFRVRGDTVEIIPVYE"
BASE COUNT         1147 a         2318 c         2362 g         1092 t
ORIGIN      
        1 gcgtagatct gcgtcgcgac gcccgtcacc gcccccgcac gcgcggcgcg gtggtgcgtg
       61 atgttcagat caacgcccac ggcgatcccg tccgggcccg cgtgtaccat cgccgcgagg
      121 ctccccaggg tctcggcgag cgcggccgtg gcgcccccgt ggaggagccc gtagggctgg
      181 gtgttgccgt cgatgggcat ggtcccgacc gccctcgcgg ccgaaacctc ggtgacctca
      241 atgccgagcc gctggatcaa ggtgccgggg ggaatctgcg cgagaaccgc tgccgtggcg
      301 tcgtccatgg gacctagagt ggcatgtgtg agcgaatcgc gacagggcag cctcctcctc
      361 atcgacggct tctcgatggc cttccgcgcg ttcttcgcgc ttcctgcgga caacttccaa
      421 acctccacgg gcgtgcccgt caacgccgtg tacggattcg cgtcgatgat cgtgtccctc
      481 atcgcggacc acaagccgac gcacatggcc gtggcgttcg acctgccggg gggcacgttt
      541 cggaccgtcc gctatccgcc ctacaagggg acgaggggcg agaccccccc ggagttcgtc
      601 ccacagatcc cgctcctgcg agaggtcctc gccgcgctcc ggattcccgc gatcgacaag
      661 gtgaactacg aggccgacga catcctcgcg acctacgccc gccagggcgc ggagcaaggg
      721 ttcgaggtct tcgtggtctc cggggaccgc gacacgcttc agttggtcac ggaccgggtc
      781 acggttctct acccccgcaa gggggtctcc gacctcgcgg tgatgacgcc ggaggccgtc
      841 cgcgagaagt atggcgtcgc gcccgccaag taccccgacc tcgcggccct ggtgggcgag
      901 acgagcgaca acatcccggg cgtcccgggc gtgggaccca agaccgcggc gaagtgggtg
      961 gagacctacg gcgggctcga ccagatcctt gcggccgctg gcgacatcgc gggcaaggtg
     1021 ggggagagcc ttcgggagaa ccttgagcag gtggcgctca acagggagat caaccacctg
     1081 ctcaccgatc ttgacctgcc gctgtcgatc gatgatctag tgagggtggg cggcgacgcc
     1141 gccaccatcc acgccctgtg cgacgccctc cagttccggg gccttcggcc ccgtcttctc
     1201 gccttggccg cggacgcctc cgaccccgcc gcgccggtgg acgggtcggg ggatccggcc
     1261 gtggtgaccg agggcgccat cgaggccatc gcctctctcc cgggccccgt ggccatggcg
     1321 ctcgttggcg gcaaggaccc ccgcgcgctc ggcgtcgctt caccgggggg tggagtttgg
     1381 gcggtcgacc tgacctccct tacccccgtc gaggagcagg cgatcggcgc gtggctctcg
     1441 tccgcatcgg tccccaaggt gcttcacggg gcgaaggccg cttggcacgg gctcgccgcg
     1501 cgcggatacg aactcgcggg tatcgcggga gacacggaga tcgccgcgta cctcgtccag
     1561 cccgaccagc gcgggttcga cctcgaggac gtgatgcaga ggtacctcgg cgtcgccgtc
     1621 gccgggtcga agagcgacga gctcgacctg gggctcggct cttcggaggc gcacgtcgcc
     1681 gggcttcggg cgtggacggt ctcgcgcctc gccgaggaac tcgagggtgc gcttgactct
     1741 cgcaagatga cctcgctcta tcgcgaactg gagattcccc tgatcggcgt gctcgcggcg
     1801 atggaggccg cggggatcgc cgtggacggc gcgcacctga ggatgctggc gggggagtgt
     1861 tccgcccggg ccgagagtgc ccagcgcgag gcgtggcgcg cgatcggcgg cttcgaggtc
     1921 aacctgggtt cacccaagca acttcaggag gtcctcttcg accgcctcga catgccgaag
     1981 accaagaaga tcaagacggg gtacaccacg gacgccgcgg cccttgcgga gctctacgag
     2041 aagcgcccgc acccgttcct cgaggcgctg ctcgcgtatc gtgccgccgc gaagctcggg
     2101 cagatcatcg agacgctcgc gtcggccatc ggcccggacg gtcgcattca caccacgttc
     2161 tcccaggtcg tcgcgtccac cgggcgcctg agctcgctgg accccaacct tcagaacatc
     2221 cccatccgca ccgatgacgg ccaccgcata cgtgaggcat tccgggtagg ggagggctac
     2281 gagacgctcc tgacggcgga ctacagccag atcgagatgc gaatcatggc gcacctgtcg
     2341 ggcgacgacc aactgatcga ggcgttccga tcgggcgagg acctccaccg tttcgtgggc
     2401 ggcagggttt tcggcgtcca gccggagaac gtcacgccgg agatgcgctc gaaggtcaag
     2461 gcgatgtcct acggcctcgc gtacgggctc tcgtccttcg ggctcgcccg ccagctggga
     2521 acgtccacgg gcgaggcgca ggcgctgatg gacgactatt tcgcgcgatt cggcgaggtg
     2581 cgggactacc tcgcttctgt tgtccgcgag gctcgggtca cgggctacac ggagacgatc
     2641 ttcgggcggc ggcgctacct tcccgacctg aactcgacgg atcgtcagcg ccgcgacatg
     2701 gcggagcgga tggcgctcaa cgcgcccatc cagggcagcg cggccgacct gatcaaacgc
     2761 gccatgatcg gcgtctcgcg ccggttggag gcagagagtc tcgcgtcgcg acagatcctg
     2821 caggttcacg acgaactcgt ggtcgaggtc gcgaggggcg agaagagcgc ggtcgaggcg
     2881 atcctgcgcg aggagatggc tggcgcggcc gacctgagcg ttcccctcga cgtccacctc
     2941 gggtccggcg agaactggcg gattgcggga cattagtcgc cggtggggtc gtttcacggc
     3001 actaggctgg gcccatgcgc atcggagtcc ttacaggcgg aggggactgc cccggcctca
     3061 acgcggccat cagggccatc gtcaagcgtg gctcatccga gcacggccac tcgatcgtcg
     3121 ggttcaggga cggatggcgc gggttcctcg acggcaactc cattcccctc gatcgcaacg
     3181 acgttcgcgg ggtcctcgtc cacggcggaa cgatgctggg tacggcgcgc tgccatccgg
     3241 acaaggagcc ggggggcctt gagcgcgtgc gggaggtcgt cgaggcggag aggctcgacg
     3301 ccatcatctg catcggaggc gacggcaccc tcaaggccgc ctccagggtc gcggacacgg
     3361 gcatccccgt tatcggaatc ccgaagacga tcgacaacga cgtagtcgga acggacgtct
     3421 cgatcggctt cgataccgcg atgatgatcg cgacggaggc gatcgaccgg ctgcactcca
     3481 ctgccgagtc gcacaaccgg gtcatggtcg tcgagctcat gggacgccac tcaggctgga
     3541 tcgcggtcgc tgctggcatc gctggcggcg ccgacatcat cctcgcgccg gaggagccgt
     3601 tcgacatgga gaaggtcgcc aagcgcatca cctaccggca ccgctctcag gcgggatttt
     3661 cgattgtcgc ggtggccgag ggcgcgcgcc cctccgagga atcggactgg tcgctcgata
     3721 cgacgacgga cgaggccggg aaccccatcg cgggcgagat cggacagcag gtgacgcgcg
     3781 agattcaggc gcggaccggc tacgaggctc ggctgaccgt gctgggatac gtccagcggg
     3841 gcgggacacc cacggcggcc gaccgcctgc ttgcgacgcg catgggcgtc gcggccatcg
     3901 acgccgcgac cctgggcgtc accaagaagt tcaccgccgt gcgcgggccc gagatcgtcc
     3961 tcgaggactt cagcgtgatg gtgggcaaga ccaggttcgt tccgaaggaa ctgctggacg
     4021 ccgcgcgggg gctgtagtcg gccgacacgc gtccaccggc ctttggcggg cagcgccttc
     4081 caggggtacg attgcgggcg gcgtagcgtg cctaggcccg ctgatccctg tccctgtcca
     4141 tgcgattcac ttctgcccat cggagttttc tccctaatgt ccccctctcc ctccaagacc
     4201 cccacagttg ccgtcaatga cattggttcg gccgaggatt ttctcgccgc cgttgacgcc
     4261 accatcaaga acttcgacga cggagacctg gtcaccggcg tcatcgtcaa ggttgaccgc
     4321 gacgaggttc tgctcgacat cggctacaag accgagggcg tcatcccctc gcgcgagttg
     4381 tcgattcggc acgacgccga cccggatgac ctggtcaagg tgggggacac gatcgaggcg
     4441 ctcgttctca ccaaggagga caaggaaggg cgactcatcc tctccaagaa gcgcgcccag
     4501 tacgagcgcg catggggcac gatcgagaag atcaaggaag aggacgggat cgttaccggt
     4561 accgtcatcg aggtcgtcaa gggcggcctg atcatggaca tcggccttcg cggcttcctg
     4621 ccggcctcgc tcgtcgagat gcgccgcgtt cgcgacctcg gcccgtacat cggccaggag
     4681 ctcgaggcga agatcatcga actcgacaag aaccgcaaca acgtggtcct gtcgcgccgc
     4741 gccttcctcg agcagacgca gtccgaggtg cgctccacct tcctcaccgc gctcgctccc
     4801 ggacaggtgc gcaagggcgt cgtgtcctcc atcgtcaact tcggcgcgtt cgtcgacctc
     4861 ggcggagtgg acggcctcgt gcacgtctcg gagctctcgt ggaagcacat cgatcacccg
     4921 ggcgaggttg tcacggttgg ccaggaggtg gacgtcgagg tcctcgagat cgacatggac
     4981 cgcgaacgcg tctcgctgtc gctcaaggct acgcaggagg acccgtgggc ggtctacgcg
     5041 cgcacccacg cgatcgggca gatcgttccg ggcaaggtca ccaagctcgt tcccttcggc
     5101 gccttcgtgc gcgtcttcga gggcatcgaa ggcctggtgc acatctccga actctcaacc
     5161 cgtcacgtcg agcttcctga gcaggtcgtt tcgcccgagg atgcggtgtt cgtcaagatc
     5221 atcgacatcg acctcgagcg ccgtcgcatc tcgctcagca tcaagcaggc gaacgagggc
     5281 gtcgaccccg agggcgagga cttcgacccc gcgctctacg ggatgacggc cgagtacgac
     5341 gccgaaggaa gttacaagta ccccgagggc ttcgattcgg cgacgaacga gtggcgcgac
     5401 ggattcgatg agcagcgcga actctgggag gccgactacg gccgcgcgca cgaccgctgg
     5461 gtcgcgcaca aggccttcgt cgccaagcac gaggcggtca cggccgaact cgaggaatcc
     5521 tcacccacac ccaagaagcg ccgccctgcc gctggcccga gttcgtactc gtcgtccgaa
     5581 gagggcgagg aggacgccgg taccttggcg tcggacgagc gtctggccgc gctgcgggag
     5641 aagctcaccg gagagtaatg ctccgtatcg gcctcacggg cggaatcgct tcgggcaagt
     5701 ccgttgcgtc cgagcggttc gcccaactgg gagcgcgggt catcgaccac gatgacctcg
     5761 cgcgccaggc tgtccaaccc gagtctgccg cgctggccga cattgcgcgg gagttcggcg
     5821 atcgcctcat cacgcggggg aagctcgacc gcgcggcgct ggcggacatc gtattcggcg
     5881 acccggaggc gcgcgcgaag gtgaacgcca tcgtgcatcc gtacatcttc gcgctggcga
     5941 aggccgcgga cagccaggcg cggctcgacg gcgtgaaggt gatcgttcat gcgattccgc
     6001 ttctggtcga gaccgggctg cgggggtcat gggacacggt cgtcacgatc gccgctccgg
     6061 ccggggtgag gaccgcccgg ctcgtgaagg agcgggggct caccgccgcg caggccgagt
     6121 cgcgcctctc ggcccaggtg acggacgacg agcgggcgcg atgggccgac atcgtccttg
     6181 acggcggggg aacggtcgcg gaattgcgca agcaggtcga tcgcttctgg aaggaccacg
     6241 ttcccgtgtc ggcgcgctga cgtagcctgg gacgatgacc gcgcccgacc tccgacgctc
     6301 cgaggcgccc ttccgcgtca tctccgacta cacgccctct ggcgaccagc ccacggcgat
     6361 cgacgagctc gaggcgcgag tccgggcggg cgagcgggac attgtgctgc tgggcgcgac
     6421 gggcacgggc aagtccgcaa cgacggcgtg gctgatcgag cggcttcagc gcccgactct
     6481 cgtgctcgcc cacaacaaga ccctcgcggc gcaacttgcg aacgagtttc gcgaactcct
     6541 ccccaacaac gccgtcgagt acttcgtctc gtactacgac tactaccaac ccgaggccta
     6601 cgtgccccag acggacacgt tcatcgagaa ggacagttcg atcaacgagg aggtcgagcg
     6661 actgaggtac tcggccacca actcgctcct gacacggcgc gatgtcgtgg tcgtgtcgtc
     6721 cgtgtcgtgc atctacgggc tgggaacgcc agaggagtac atccgcggga tggcgacgct
     6781 cgaggtcggg cagcagatcg agcgggacac gctgctacgg aagttcgtcc gcatgcaata
     6841 cacgcgcaat gacctcgcct tcactcgcgg gaccttccgg gtccgcggcg acacggtcga
     6901 gatcatcccc gtgtacgag
//
LOCUS       DASVKP010000136         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1284937, whole genome shotgun sequence.
ACCESSION   DASVKP010000136 DASVKP010000000
VERSION     DASVKP010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284937"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2023)
                     /locus_tag="VF362_02770"
     CDS             complement(<1..2023)
                     /locus_tag="VF362_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589881.1"
                     /translation="MRWRNPVLLGLAPAGAAIAAAAIVVAVQLLVLLGGGGHVATWLP
                     FSPFSGLAVIIGGIALAAPGLWRPLPRWLEPVAAVGSVFVVVGVTEAAYSVAIHTWPW
                     QVWAVSACFTVGSIACVLSALLWRAVAARWVAGIAATGLATIAGIDGAWAFGGAEIPA
                     PQGLACALIPLALLVAFGRRWPRVTLAPAALVASAVGAGTAYVAVGTRPAAITGAILV
                     VAVILWVGAWLPSGWRSPILVGIAPAFAVALAVGLGSIIPAIAGLSGNNVSDARLAYD
                     VWTSGTTVLAGLALAAPGRWKPRPRWLRYVGVVGSVLVVMGITEAAYWVAIHAWPGEV
                     WAVSACFTVGAVVCVVSALLWQSVAARWVAGIAATILATIAGIDGAWAFGVAEIPAPP
                     ALACALVPLALLVAFGRRWPRVTLGPAALIASALGAGTAYLTVGTRPAAMTGAILVVA
                     VIVWAGVRLPADWKAPVLLGATPALALALAVGVGSVIPVLAGLAANQVPDARWAYDPW
                     TSATTALAGIALGALPRWHIGERLAGAMSLVGSLVVTAAAASAAVSITDSWGGDAHQG
                     LAVNVAIFSLASAALTMLWATRAARLAHGIGSTVLLTIAGIQGSVAFSEPSASLWLGL
                     AVVVAPIVVLVVFARWWPAITLGAASFLFTLAIFAVNRHAEMPDASLFGM"
BASE COUNT          395 a          794 c          755 g          189 t
ORIGIN      
        1 ccatgccgaa gagcgaggcg tccggcatct ccgcgtgacg gttcaccgcg aagatcgcga
       61 gggtgaagag gaacgacgcg gctccaaggg tgatggcggg ccaccagcgg gcgaacacga
      121 cgaggaccac gatgggcgcg acgacgacgg cgagcccgag ccagaggctc gccgagggct
      181 cggagaaggc cacggagccc tgaatgcccg cgatggtgag aagcacagtc gagccgatcc
      241 cgtgcgcgag ccgcgccgcc ctcgtcgccc acagcatggt gagggcggcc gaggcgagcg
      301 agaagatggc gacgttgacg gcgagaccct gatgggcgtc cccgccccag ctgtcggtga
      361 tcgagaccgc cgcagaggcc gcggccgccg taacgacgag ggatcccacg agggacatcg
      421 caccggcgag gcgttcgccg atgtgccatc gcgggagcgc cccgagggcg atgcccgcca
      481 gggcggtggt cgcgctcgtc cacgggtcgt aggcccagcg ggcgtcgggc acctggttgg
      541 cggcgagccc cgcgaggacg gggatcacgc tccccacgcc gacggcaagc gcgagggcca
      601 gagcgggagt cgccccgagg aggaccggag ccttccaatc cgcgggaagg cgcaccccag
      661 cccaaacgat gaccgccacg accaggatgg cgcccgtcat cgccgccggc cgcgttccca
      721 ccgtgagata cgcggttccc gcgcccaggg cggacgcgat cagcgccgca ggccccaggg
      781 tcacccgcgg ccacctgcgg ccgaacgcga cgaggagcgc aagcggaact agggcgcacg
      841 cgagggccgg gggcgccgga atctcggcga cgccgaaggc ccacgccccg tcgattcccg
      901 cgatcgtcgc gaggatcgtc gcggcgattc ccgcaaccca ccgcgccgcg acggactgcc
      961 acaggagcgc agagacgacg cacacgacgg cgccgacggt gaaacacgcc gataccgccc
     1021 acacctcgcc tggccaggcg tggatcgcga cccaataggc ggcctccgtg atgcccatga
     1081 caacgaggac cgacccgacg acgccgacgt accgcaacca ccgcggtcgg ggcttccatc
     1141 gccccggggc ggcgagcgca agcccggcaa gaacggtggt cccgctcgtc cacacgtcgt
     1201 aggccagccg ggcatcggac acgttgtttc ccgacagccc cgcgatggcg gggatgatgc
     1261 tgcccaaccc gaccgcgagc gcaaccgcga acgctggcgc aatcccgacc aggatcggac
     1321 tcctccaccc ggagggcagc cacgccccca cccagaggat gaccgccacg acgaggatcg
     1381 cacccgtgat cgccgcgggc cgcgttccca ccgcgacata cgcggttccc gcgcccacgg
     1441 cggacgcgac gagcgccgcg ggagccaggg tgaccctcgg ccacctgcgg ccgaaggcga
     1501 caaggagcgc gaggggaatc agggcacatg cgagaccctg cggcgccgga atctccgcgc
     1561 cgccgaaggc ccacgccccg tcgatacccg cgatcgtcgc gaggccggtc gcggcgattc
     1621 ccgcgaccca ccgcgcggcg actgcgcgcc acagtagcgc cgagagaacg cacgcgatcg
     1681 agcccacggt gaaacacgcg gacaccgccc acacctgcca cggccacgtg tggatcgcga
     1741 cggaatacgc ggcctcggta acgcccacca cgacaaacac cgaacccacg gcggcgacgg
     1801 gttccaacca gcgaggcagt ggcctccaga ggccgggggc cgcgagcgcg atgccgccga
     1861 tgatgaccgc gagcccgctg aacggactga acggaagcca tgtggcgaca tgacctccgc
     1921 cgccgagcag gaccagaagc tgaacggcga cgacgatcgc ggcggcggcg atcgccgcgc
     1981 ccgccggggc gaggccgagg agcacggggt tgcgccatct caccggagca atcgcgcacg
     2041 ccgcggcgat caccgccgtc gccccagcga cggccgcgac cgtctgcgcc tgggttgctc
     2101 ccccgcccca gaagagcgac gcgacgacgg tcg
//
LOCUS       DASVKP010000137         6872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1036007, whole genome shotgun sequence.
ACCESSION   DASVKP010000137 DASVKP010000000
VERSION     DASVKP010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036007"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..956
                     /locus_tag="VF362_02775"
     CDS             171..956
                     /locus_tag="VF362_02775"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX7589882.1"
                     /translation="MTSRAAFADGVRDVAGLVPGVAVFGLSYGALIRSRGIDPIAGAV
                     SSLIVGAGAGQTAAVEVVVAGGTIAIAVLSALVVNARFALYSAALAPMFGRFSRPWRW
                     GLAYLVSDQTVGLYGRGQERWTTPALQQYYLLGVTAPMRTAWVGGTAAGILLGPVVPD
                     AWQIGVIVPLMFIALAIPSIRGLPELVAGVTGVVAVVALKDLPLGLNIVAAALLGMTA
                     GLLVRPGARSRGARGLGQSGPPSAPTPSPAPAPAHPQDSADST"
     gene            953..1267
                     /locus_tag="VF362_02780"
     CDS             953..1267
                     /locus_tag="VF362_02780"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX7589883.1"
                     /translation="MKIWLIFILAAIGTYAIRVSGLALFRDEDRMSPLVRRALRMIGP
                     AAMGAIVGNSLLLEGGALRPFGAWHIAAIVAVGFAAWKRNLALTMLAGAAVFAVLLLV
                     GF"
     gene            1464..2138
                     /locus_tag="VF362_02785"
     CDS             1464..2138
                     /locus_tag="VF362_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX7589884.1"
                     /translation="MDVGEDQPGAQVSNILGHILSAPSGVVYALVGLVVFAEDALFIG
                     FVLPGETAAILGGVAASQHHVGLVAILAVVVAAAIVGDTVGYEIGEHLGARILNIPML
                     HRHRHRLSDAQLLLARRGGWAVFLGRFIAFFRAMMPALAGTARMPYPRFLAFNAAGGV
                     VWGTGVVLVGYLAGNSYQTVEKTVGRGFAIAVAVIVVSALIVWRVRRQRAEAALEALP
                     DKTADA"
     gene            2323..2559
                     /locus_tag="VF362_02790"
     CDS             2323..2559
                     /locus_tag="VF362_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589885.1"
                     /translation="MEIRPSALKHGVRADDIRHAYQNAIRYVEFEYRGETRLLVIGAD
                     SAGRLLELVVVPSPETSRIIHADLLRPKFRAYVR"
     gene            2564..2812
                     /locus_tag="VF362_02795"
     CDS             2564..2812
                     /locus_tag="VF362_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589886.1"
                     /translation="MTKLSERVQADAGLHAVDPARAGARDARHFRRIIAARRGIADAE
                     AELRAAVAAARAAGDSWTVIGAALDTSRQAAFQRFGRN"
     gene            2865..3242
                     /locus_tag="VF362_02800"
     CDS             2865..3242
                     /locus_tag="VF362_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7589887.1"
                     /translation="MYIAKDLVAASAAPMVLGILADAESYGYAILARVRELSGGHIEW
                     TDGLLYPLLHRLEEQGLVEATWGAAENGRKRKYYRITPQGRDSLAEHRRQWRTVSDAL
                     QETWSNVKEAAAWAARPAEGMAT"
     gene            3239..4618
                     /locus_tag="VF362_02805"
     CDS             3239..4618
                     /locus_tag="VF362_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEX7589888.1"
                     /translation="MKTSDAEAGFEAQIAQWREFILKRRGVVTADADELESHLRDQAA
                     DLMASGLSPDEAFLVAVKRLGAQDEISREFARVHSGRLWKQLVLAGADAPGPAAGANE
                     SRTELRWVIGLAVLAAASFKVPALFGVSQMDNPDFFARNAALFFLPSLAAYLAWKRRA
                     SGRTIGVLVGAFIAGGIFANAYPFHSGGQTELLSGLHLPMALWLVTGIAYVGGDWRSH
                     SRRMDFIRFTGEWVIYMSLIALGGGVLAGITAGAFGLFGVDTTAFIVEWLVPCGAMGA
                     AIVAAWLVEAKQGVIENLAPVLTKIFTPLVAALLIALVVTIPITWTGKPIDRNALIVL
                     DLLLALVLGLVLYAISARDASEAPGLFDRLQFVLVLAALVVDAFVLAGLLGRIGEYGF
                     TANRVAALGENLILLVNLAWATWLALGFLRHRRTLGATERWQTSYVPVYLAWAVAVVA
                     LFPPIFSFA"
     gene            4709..>6872
                     /locus_tag="VF362_02810"
     CDS             4709..>6872
                     /locus_tag="VF362_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEX7589889.1"
                     /translation="MRTTAKKARDIVAALRPEDKIRLVSGHDFWTTEAIPEAGIRAMT
                     LTDGPHGLRKQRDAGNVADVTDSVPATCFPTAAALASTWDPALLEEIGAALGSECRAE
                     DVPVLLGPALNLKRHPAAGRNFEYLSEDPVVAGRMAAGLVRGIQSQGVAACLKHFAAN
                     NQESWRMSIDAIVDERTLRELYLTGFEIAVRESDPWTVMCAYNQINGTHCGENHGLLT
                     EILRDEWGFDGLAMTDWLATFDRVAAIRAVLDLEMPGSAATWDLEIAAALDAGTLAEA
                     DLDLACARIVDLMLRTAPASATGGTDAPGAAFAAPDFAAHHALARRAAAAGTVLLTND
                     GLLPLGAATTIAVVGAFAERPRYQGAGSSLVTPTRLDTFLGALREQAGESRVTYAPGY
                     DPITGETTDALLAEAARVAAGADAVVVLAGLPSRMESEGFDRDHLRLPEGHVRLIDAV
                     IDANPRTAVALQNGAPVEMPWVARPAAILEAYLGGQAGGGALADVVLGAVEPGGRLAE
                     SFPVRASDLPSSANFPGTATQVQYREGLYVGYRFHDASGVAPLFPFGHGLGYTDFAYS
                     GLTVTEVGARWDVTVTVKNVGTRAGSDVVQVYVQDVVSTAYRPVKELKAFAKVHLAPG
                     ATQNVTVSLDRRAFAVWDVASHGWRVEAGDFDILVGASSTDIRAVRRIAVASPDAVAA
                     SSGPTAPTYLVGDAEFARMLGHPIPAAAPLLPFHRDSTV"
BASE COUNT          917 a         2523 c         2309 g         1123 t
ORIGIN      
        1 acgacgcggg cgtgtccatt gtcgaactgg atgcagtcgg agtacccgtg gaagtccacg
       61 cgggatgtgc tcacggccat gcacccattc tcccgcgagc cgcgagcggg ggtgggcgcc
      121 tcgggagggc cgtgcctacc cgcgtcggcc ggggcaatac gatcgacgac atgacctccc
      181 gcgccgcctt cgcagacggc gtccgcgacg tcgcgggcct ggtgcccggc gtcgccgtgt
      241 tcgggctctc gtacggcgcg ctcatccgct cgcgcggcat cgacccgatc gccggggcgg
      301 tcagttcgct catcgtcggg gcgggagccg ggcagaccgc ggccgtcgag gtcgtcgtcg
      361 ctggcgggac catcgcgatc gcggtgctct ccgccctcgt cgtcaatgcg cggttcgcgc
      421 tgtactcggc ggcgctcgcg cccatgttcg gccgcttcag ccgcccctgg cgctggggcc
      481 tcgcgtacct cgtctccgac cagaccgtcg gcctctacgg gcgcgggcag gagcgctgga
      541 cgacgccggc gctccagcag tactacctgc tcggggtcac ggcgccgatg cgaaccgcgt
      601 gggtgggcgg gacggcggcg ggaatcctgc tcggaccggt ggttccggac gcgtggcaga
      661 tcggcgtcat cgtcccgctg atgttcatcg cgctcgcgat cccgagcatt cgcggccttc
      721 ccgaactcgt cgccggcgtc accggcgtgg tggcggtcgt cgcgctcaag gacctgccgc
      781 tcgggctcaa catcgtcgcg gcggcgctgc tcggcatgac ggccgggctg ctcgtgcgcc
      841 cgggagcgag gagtcgagga gcgaggggcc tggggcagag cggtccgcct tccgcgccaa
      901 cgccctcccc cgcgcccgca cccgcccacc cgcaagactc ggcggactcc acatgaagat
      961 ctggctcatc ttcatcctcg ccgcgatcgg cacctacgcc atccgcgtct ccggcctcgc
     1021 gctgtttcgc gacgaagatc gcatgtctcc cctcgtccgc cgcgcgcttc gcatgatcgg
     1081 cccagccgcg atgggcgcca tcgtcggcaa ctcgctcctg ctcgagggag gcgcattgcg
     1141 ccccttcggc gcgtggcaca tcgccgcaat cgtcgccgtc ggcttcgcgg cgtggaagcg
     1201 gaacctcgcg ctgacgatgc tcgccggtgc cgcggtgttc gcggtgctcc tgctcgtcgg
     1261 gttctaggcg gcgcgttcgc ggcgggtccg caacgggtcc gcaacgggtc cgcggagact
     1321 cgctccgaga cgcggcccgc ccccgtctat cgagcggagt gatgtagcgt ttccaccaca
     1381 cccgattccc ttccgagggt aggcagtcgt cttccccgga tcggccgccg acccgtgagg
     1441 aacaccgtgc ccggtttggt cttgtggacg ttggcgagga tcagccgggg gcgcaggtga
     1501 gcaacatcct cggccacatt ctgtctgcgc ccagcggcgt cgtctacgca ctcgtcgggc
     1561 tcgtggtctt cgctgaggac gcgctgttca tcgggttcgt tctgcccggc gagaccgcag
     1621 cgatcctggg cggggtcgcc gccagccaac accatgtggg ccttgtcgcc atcctcgcgg
     1681 tcgtcgtcgc ggccgccatc gtcggcgaca cggtcggcta tgagatcggt gagcacctcg
     1741 gtgctcgaat ccttaacatc ccgatgctcc accgacaccg acaccggctg tccgacgccc
     1801 aactcctcct cgcgcgccgt ggcggctggg ccgtgttcct cggccggttc atcgcgttct
     1861 tccgcgcgat gatgcccgcg ctcgctggca ccgcccgaat gccctacccg cgcttcctcg
     1921 ccttcaacgc cgccggtggc gtggtctggg gcaccggcgt ggtgctggtc ggctacctcg
     1981 ccggcaactc ctaccagacg gtcgagaaga ccgtcggtcg cggcttcgcc atcgcggtcg
     2041 cggtgatcgt ggtcagcgcc ctgatcgtct ggcgagtccg tcgccagcgc gccgaagcgg
     2101 ctttggaggc cttacccgac aagaccgccg acgcctgagt cacgtccaca ccccccatct
     2161 tcctgagcca ccgcccccgg acgacgcgcc gtgatccaca gatcccggat tcggcctccg
     2221 ttgctcaccc tggcgccgta ggttggcccg cgtcggtgtc ccgccgacag gacgtaaaga
     2281 tgctctttac gctcacgcgc ccgtcaaggt agggttgaca cgttggagat ccggccgtcg
     2341 gctctcaaac acggcgtccg cgccgacgac atccggcacg cgtaccagaa tgcgatccgg
     2401 tatgtcgaat tcgagtatcg aggggagaca aggttgctgg tgatcggtgc cgattcggcg
     2461 gggcgactcc tagaactcgt cgtcgtcccg tctcctgaga ccagtcgcat cattcatgcg
     2521 gaccttctac gaccgaagtt ccgcgcgtac gtgaggtgag gggatgacga agctcagcga
     2581 acgggttcag gcggacgcag ggctccacgc cgtcgatccg gcgcgtgccg gcgcccggga
     2641 cgctcggcac ttccggcgca tcatcgccgc gcgtcggggc atcgcggatg ccgaggccga
     2701 gttgcgcgcg gccgtcgcgg cggcgcgcgc ggcgggagac tcgtggacag tcatcggggc
     2761 ggcgctcgac acatcgcgcc aggccgcctt ccagcgcttc ggccggaact agccacctcc
     2821 gatgcgcgct tgccatacct agtagttcta tgtacggtag ttctatgtac atcgccaagg
     2881 acctcgtcgc cgcgtcggca gcccccatgg tcctgggcat cctcgccgac gcggagagtt
     2941 acgggtacgc catactcgcc cgggtgcgcg agctgagcgg cggccacatc gagtggaccg
     3001 acggccttct gtacccgctc ctgcatcgac tcgaagagca gggcctggtc gaggcgacgt
     3061 ggggcgcggc cgagaacggc aggaagcgca agtactaccg gatcacgccg cagggccgcg
     3121 attcgctcgc ggagcaccgg cgacagtggc gcaccgtcag cgacgccctg caggagacct
     3181 ggagcaacgt caaggaggcc gcggcatggg ccgcgcgccc cgcggaaggg atggcaacgt
     3241 gaagacgtcc gacgccgaag ctggcttcga ggctcagatc gcgcagtggc gcgagttcat
     3301 tctcaagcga cggggcgtgg tgacggccga cgccgacgag ctggagtccc acctgaggga
     3361 ccaggcggcc gacctgatgg cttcggggct ctcccctgac gaggcgttcc tcgtcgcggt
     3421 caagcgcctg ggcgcccagg acgagatctc gcgcgagttc gctcgcgtgc attccggaag
     3481 gctctggaag cagctcgtgc tcgcgggggc ggacgcgccg ggtccggccg ctggcgccaa
     3541 cgagtcccgc accgagttgc ggtgggtcat cggcctcgcg gtgctcgccg cggcatcctt
     3601 caaggtgccc gcgctcttcg gggtgagtca gatggacaac cccgacttct tcgcccgcaa
     3661 tgccgccctt ttcttcctgc cctccctcgc cgcctacctc gcgtggaagc ggcgcgcgtc
     3721 gggccgcacc atcggcgttc tcgtcggggc gttcatcgcg ggcggcatct tcgcgaacgc
     3781 atacccgttc cactcgggcg gccaaaccga actcctgagc ggcctccacc tgcccatggc
     3841 gctgtggctc gtcacgggca tcgcctacgt cgggggcgac tggcgctcgc actcgcggcg
     3901 gatggacttc atccgcttca ccggcgagtg ggtcatctac atgagcctca tcgccctggg
     3961 cggaggcgta ctcgccggaa tcacggcggg ggccttcggc ctcttcggcg tcgataccac
     4021 cgcgttcatc gtggagtggt tggtcccgtg cggcgcgatg ggggccgcga tcgtcgcggc
     4081 gtggctcgtc gaggcgaagc agggcgtgat cgagaatctc gcccccgtac tcaccaagat
     4141 cttcaccccc ctcgtggccg cgctcctcat cgcgctcgtc gtcaccattc cgatcacgtg
     4201 gacgggcaag ccgatcgacc ggaacgcgct catcgtgctc gacctgctcc tcgcgctcgt
     4261 cctcggcctc gtgctctacg cgatctcggc gcgcgacgcc tcggaagctc cggggctctt
     4321 cgatcgcctc cagttcgtcc tcgtgctcgc cgccctcgtc gtggacgcct tcgtcctggc
     4381 cggactcctt gggcggatcg gggagtacgg cttcacggca aatcgcgtcg ccgcgctcgg
     4441 cgagaatctc atcctgctcg tgaacctcgc ctgggcaacg tggctcgcgc tcgggttcct
     4501 ccggcatcgg cgcaccctcg gcgcgacgga gcggtggcag acctcctacg ttcccgtcta
     4561 cctcgcctgg gccgtcgcgg tcgtcgccct cttcccgccg atcttctcct tcgcctagcc
     4621 gcctcgcggg tcgagtggag gcgaaacgga cgacgccgtc gtccggaccg gcacgttcgg
     4681 ggccgtagcc ggtcgctagc atggggcaat gcgcaccacc gccaagaagg cgcgggacat
     4741 cgtcgccgcg ttgcgccccg aggacaagat tcggctggtc tccggccacg acttctggac
     4801 gacggaggca atccccgagg ccgggattcg ggcgatgacg ctcacggacg gccctcacgg
     4861 cctccggaag cagcgagatg ctggcaacgt cgcggacgtc accgactcgg ttcccgccac
     4921 ctgctttccc accgccgcgg ccctggcgtc gacgtgggat cccgcgctcc tggaggagat
     4981 cggagccgcc ctcggtagcg aatgccgcgc cgaggacgtc ccggtcctcc tcggccccgc
     5041 gctcaacctc aagcgtcacc cggcggccgg gcgcaacttc gagtacttgt cggaggaccc
     5101 ggtggtcgcc gggcgcatgg ccgccggcct cgttcgcggc atccaatccc agggcgtcgc
     5161 cgcgtgcctc aagcacttcg ccgcgaacaa ccaggaatcg tggcggatgt ccatcgacgc
     5221 gatcgtggac gaacggaccc tccgcgagct ctacctcacc gggttcgaga tcgccgtccg
     5281 ggagtccgac ccgtggaccg tcatgtgcgc ctacaaccag atcaacggaa cgcactgcgg
     5341 cgagaaccac gggctcctca ccgagattct gcgcgatgag tggggcttcg acgggctcgc
     5401 gatgacggac tggctcgcta cgttcgatcg ggtcgcggca atccgcgcgg tcctcgacct
     5461 cgagatgccc ggcagcgccg cgacgtggga tctcgagatc gccgcggcgc tcgacgccgg
     5521 gacgctcgcc gaggccgatc tcgacctggc gtgcgcgcgc atcgtcgacc ttatgctccg
     5581 cacggctccc gctagcgcca cgggcgggac ggacgctccc ggggccgcct tcgccgcccc
     5641 cgacttcgcc gcacatcacg ccctcgctcg ccgcgccgcc gcggcgggaa cggttctcct
     5701 cacgaacgac ggcctgctcc ccctcggcgc cgcgaccacg atcgcggtcg tcggcgcgtt
     5761 cgcggaacgt cctcgctatc agggcgcagg aagttccctc gtcaccccga cgcgcctcga
     5821 caccttcctc ggcgccctcc gcgagcaggc gggcgagtcc cgcgtcacct acgcgcccgg
     5881 atacgacccc atcacgggcg agaccaccga cgcgctcctc gccgaggcgg cgagagtggc
     5941 ggccggagcc gatgccgtgg tcgtcctcgc cggccttccc agccgcatgg agtcggaggg
     6001 cttcgaccgc gaccacctgc gactccccga gggccacgtc cggctcatcg atgcggtgat
     6061 cgacgccaac ccccgcaccg cggttgcgct gcagaacggc gctcccgtcg agatgccgtg
     6121 ggtcgcgcga cccgcggcga tcctcgaggc gtacctggga ggccaggcgg gcggcggcgc
     6181 cctcgcggac gtcgtcctgg gcgcggtgga acccggtggg cgcctcgctg agagcttccc
     6241 cgtccgcgcg tcggaccttc cctcgtccgc caacttcccc gggaccgcca cccaggtgca
     6301 gtaccgagag gggctgtacg tcggctaccg attccacgac gcgtccggcg tcgcgcccct
     6361 cttccccttc ggtcacgggc tcggctatac cgacttcgcg tactccggcc tcacggtcac
     6421 cgaggttggt gcgcggtggg acgtgacggt caccgtgaag aacgtgggca cgcgggccgg
     6481 ttcggacgtg gtccaggtgt acgtgcagga cgtcgtgtcg acggcgtacc ggccggtgaa
     6541 ggaactcaag gccttcgcga aggtgcacct cgcgcccggc gcgacgcaga acgtcaccgt
     6601 ctcgctcgat cggcgcgcgt tcgcggtctg ggacgtcgcc tcgcacgggt ggcgcgtcga
     6661 ggcgggcgac ttcgacatcc tcgtcggcgc ctcatcgacg gacattcgcg ccgtgcggcg
     6721 gatcgctgtc gcctcccccg acgccgtggc ggcgtcgagc ggccccaccg ctcccaccta
     6781 cctcgtgggc gacgcggagt tcgcgcggat gctggggcac cccatccccg cggccgcccc
     6841 cctcctgccc ttccaccgcg actccaccgt cg
//
LOCUS       DASVKP010000138         3577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1526729, whole genome shotgun sequence.
ACCESSION   DASVKP010000138 DASVKP010000000
VERSION     DASVKP010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526729"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VF362_02815"
     CDS             <1..457
                     /locus_tag="VF362_02815"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HEX7589890.1"
                     /translation="NTLPRFASRTGLDLHPIDVDDPDQVAWLKALVWPEHTDRFALLE
                     AALDVRRTTDITMIAGDAVETLVALEESLPPGPLLAWHTVALYQFSDDQRRRLDSAFA
                     MIAERRAVARVGLEGIPNLPGHWVRVGVEQEGAPIVAHAQGHGRWVDLP"
     gene            complement(536..2260)
                     /locus_tag="VF362_02820"
     CDS             complement(536..2260)
                     /locus_tag="VF362_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX7589891.1"
                     /translation="MTKAFVAPTRRQINLIVFALMVGMFLAALDQMVVATAIRTIGDD
                     LHGLTLQAWVTTAYMIAATISTPLYGKLSDMFGRKPLYITALGLFILGSALSATATSM
                     YQLAAYRGVQGAGAGGLMALAIMILGDLLSPRERTKYQAAFFGVWGISSVLGPVIGGF
                     FAGATSILGVAGWRWIFLINVPLGAVALVLIIRVLHVHHMGRGQQRVDWWGVAALTLG
                     IIPLLTVLERGNGWGWTSQTSLALFTASVVGFALFYLAERVAGDDALLPLRMFRNRTV
                     AVSTTVNFIMGFGMFGGLAGIPLYLQISRGMSPTHAGLAMLPFTVGIGIVATASTIVI
                     RKTGHYKWIPVTGVFLMLVGVALLSRIQYDSPLWKVFGAAVIFGGGLGALMQPLMLAV
                     QNALSPRDNGAGTASVMFFRQIGGSLGTAVFLSLLFSNVQTKIANAFKAAAQTPAFLQ
                     AIRDPAVNVPGSTNAAVIAAVTSGDPTQLQSSGISLNDTSFIHNLAPQLAAPFQIGFA
                     QAIAGVMLVAGAFIVVGFVAIFFLPSLPLKEKSGLQAMADEAEEIAGVSHKGADAEAG
                     EQMAVPLG"
     gene            complement(3148..>3577)
                     /locus_tag="VF362_02825"
     CDS             complement(3148..>3577)
                     /locus_tag="VF362_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589892.1"
                     /translation="GGASGDTGSGASGDTGSGASGATGSGATSGDASETRTHGDGGGT
                     AEVDGPVGAGGGGGASGTTSVGGGKGLAPADGNPGRPEAPPALPRGAGMGSAGMGGAG
                     MGGAGMDAPAGTGAKIEYGTATGGEAPVSGPKGASDSVGA"
BASE COUNT          604 a         1187 c         1246 g          540 t
ORIGIN      
        1 gaacactctt ccgcggttcg cttcgcgaac ggggctcgac ctacacccga tcgacgtcga
       61 tgaccccgat caggtcgcgt ggctcaaggc actggtctgg ccggagcata ccgaccgatt
      121 cgcgctgctc gaggccgcac tggacgtgcg ccggacaacg gacatcacga tgatcgctgg
      181 cgatgcggtc gagacgcttg tggcgctcga agagtccctc ccgccgggtc cgctcctcgc
      241 ctggcacacg gtggccctct accagttctc ggacgaccag cgacgccgac tggattcggc
      301 gttcgcgatg attgccgagc gacgggcggt cgcgcgagtt ggtctcgagg gcatccccaa
      361 ccttccgggc cactgggtgc gggtcggagt ggagcaggag ggcgcgccga tcgtcgctca
      421 cgctcagggc cacggccggt gggtcgacct gccctgaccg cgacggagag cgaacaacgg
      481 cgtgggccga ggcccacgcc gtgtccgtca cctttgaggc cgaacctcgg gccaactagc
      541 cgagcgggac ggccatctgc tcgcccgctt ccgcgtccgc tcccttgtgc gagacgccag
      601 caatctcctc tgcctcgtcg gccatcgcct ggagtcctga cttctccttg aggggaaggg
      661 agggcaggaa gaagatcgcc acgaatccga cgacaatgaa cgctccggcg acgagcatga
      721 cgccggcgat cgcctgcgcg aacccgatct ggaacggcgc cgcgagttgc ggggccaggt
      781 tgtggatgaa ggacgtgtcg ttgagcgaga tgcccgacga ctggagttgc gtcgggtccc
      841 cggaggtgac cgccgcgatg acggcggcgt tcgtcgaacc cgggacgttc acggccggat
      901 cccggattgc ctgcaggaag gcaggcgtct gtgccgcggc cttgaacgcg tttgcgatct
      961 ttgtctggac gttgctgaac aggagcgaga ggaagacggc cgttccgagg gaaccgccga
     1021 tttggcggaa gaacatgacc gaggccgtgc ccgcgccgtt gtcgcggggg gagagggcgt
     1081 tctgaaccgc gagcataagc ggttgcatta gggcgccgag tccaccgccg aagatcacgg
     1141 cggcgccgaa gaccttccag aggggactgt cgtactggat gcgagacagg agggcgaccc
     1201 cgacgagcat gaggaagaca cccgtaaccg gaatccactt gtagtgcccg gtcttgcgaa
     1261 tcacgatcgt tgatgccgtc gcgacgattc cgatgccgac ggtgaagggc agcatcgcga
     1321 ggcccgcgtg agtcgggctc atcccgcggg agatctgcag gtagagcgga atcccggcga
     1381 ggccgccgaa catcccgaac cccatgatga agttgaccgt ggtcgagacg gcgacggtgc
     1441 ggttcctgaa catccgaagc ggcaatagcg cgtcgtcccc tgccacacgc tcggcgaggt
     1501 agaacagcgc gaagccaacc accgaggcgg tgaatagggc gagcgacgtc tggctggtcc
     1561 aaccccaccc gttgccgcgc tccagcacgg tcaggagcgg gatgatgccg agcgtgagtg
     1621 cggcaacgcc ccaccagtcg acacgctgct gaccacggcc catgtggtgg acgtggagga
     1681 cgcggatgat caggacgagc gcaacggcgc cgagtgggac gttgatgagg aagatccagc
     1741 gccagccagc aacgccaagg atcgacgtcg caccggcgaa gaagccgcca atgaccgggc
     1801 cgaggaccga cgagatgccc cagacgccga agaaggcggc ctggtacttg gtgcgttcgc
     1861 gtggggagag gaggtcgccg aggatcatga tggcgagggc catgaggccg cccgcgcccg
     1921 cgccctggac gcctcggtag gccgcgagct ggtacatcga cgtcgccgtc gcgctgaggg
     1981 ccgatccgag gatgaagagg cccagcgccg ttatgtagag cggcttgcgg ccgaacatgt
     2041 cggagagctt gccgtacagc ggcgtcgaga ttgtggcggc gatcatgtat gccgtcgtga
     2101 cccacgcctg gagggtaagg ccgtgaaggt cgtcgccgat ggtccggatg gccgtggcca
     2161 ccaccatctg gtcgagcgcc gctaggaaca tgcccaccat cagcgcgaac acgatgaggt
     2221 tgatttggcg ccgggtgggc gccacaaatg ccttggtcat gaggatcctt gctgggtcta
     2281 cgcggaccct gatcgcgggc ggttcacaag cctcaaccgc gcaagcccgg tcggtattcc
     2341 ctagtgtaga actgatcggc gacacgcccg gctctggcga ttctctccac agattttgtc
     2401 cacaggggtg tggagaacag cgggcccaac gggggcgacg gtggagaaac ctgtggggaa
     2461 agagtggaca aaaatacctt cttgaatcgg cttgcggtgg cacgcggaga ggactagacc
     2521 tgtccctatc gggatcgacc agcaccatcg agggaccggg caactgggcc ttcaggtcga
     2581 gggagatctc gggttatccg gacaacggcg atccacgtcg cggctccgcc cctcggggtg
     2641 aggccaagaa ctgtgggtga cggcacaggg gttgacgggc ccggatgatg gaccgagagg
     2701 ttgctccgca aggggcagcg ccgcatcgtc aatcgcggca gtcgttccgc cgggcaactg
     2761 gcgtgtcgag aactcgaggt catggcaagg cccctcggac acttactccg aggggccttc
     2821 gccatgtcac cgcgactcgc gcctcgggac actcctacgg taggtgggga tgggacctcc
     2881 ggtccggagc gacacgcggc cggtgagggc atccgctcgg cgaagtcgca cccgcgtcgc
     2941 gcgccgcctc gccccccgct tcgcgccgcg gagttcaacc tccggagcac gcgcgttggg
     3001 cggcgcgctc gcgggatcta ggccgacggg ccgacgccgc gcgcgcccgc gcgggtagca
     3061 tcgtgccaag tgcgaagagg aggcagggat gacgactccg ccgccagggc acggcgagga
     3121 gagccagaac cccccgccgc cgttgcctca ggcgccgacc gagtccgacg cgccctttgg
     3181 tccggagacc ggcgcctccc cgcccgtagc tgtgccgtac tcgatcttcg ctccggtacc
     3241 ggcgggcgca tccatgcccg cgcctcccat gccagcgcct cccatgcccg cgcttcccat
     3301 gcccgcgccc ctcggcaagg cgggcggtgc ctcgggtcgc cccggattcc cgtcggcagg
     3361 agcgaggccc ttcccgcccc cgaccgacgt cgtgccgctt gccccaccgc cacctcccgc
     3421 gccgaccggc ccgtcgactt ccgccgtccc cccgccgtcc ccatgggttc gagtctccga
     3481 cgcgtcgccg ctcgttgcgc cgctgcccgt ggcgccgctt gcgccgctgc ccgtgtcgcc
     3541 gcttgcgccg cttcccgtgt cgccgcttgc gccgccg
//
LOCUS       DASVKP010000139         5086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_708169, whole genome shotgun sequence.
ACCESSION   DASVKP010000139 DASVKP010000000
VERSION     DASVKP010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708169"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1229)
                     /locus_tag="VF362_02830"
     CDS             complement(105..1229)
                     /locus_tag="VF362_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEX7589893.1"
                     /translation="MSTDIEIGRGKRGRRAYAFDDIAVVPSRRTRDPEQVSLRWQIDA
                     FQFEIPIIAAPMDSVMSPATAIALGKLGGLGVLDLEGLWTRYEDPTPYLIEIASLGSD
                     VATRRMQEIYRTPVDADLIKLRLGEIRAAGVTVAGALSPHRTQEFYKTVLAAGVDVFV
                     IRGTTVSAEHVSGEAESLNLKEFIYDLDVPVIVGGASTYQAALHLMRTGAAGVLVGFG
                     GGAAHTTRTTVGIHAPMATAVADVAAARRDYLDESGGRYVHVIADGGLGRSGDMVKAI
                     ACGADAIMVGAALARAAEAPGGGYHWGPEAHHAELPRGERVYVGTVGPLAEIVFGPGS
                     RADGTTNLMGALRRSLATTGYSDLKEFQRVDVVISPYEPH"
     gene            1290..2009
                     /locus_tag="VF362_02835"
     CDS             1290..2009
                     /locus_tag="VF362_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016663901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX7589894.1"
                     /translation="MSWLERTMVGFDTETTGVDSATDRIVTAAVIFRDGADGAVSAHT
                     WLIDPGVEIPERATAVHGITTEMARANGADPVVALGEIAALLAAALANGAPIVAFNAG
                     YDLGILDAELARHGLTSLARRIGAAHTLRPVVDPLVLDRHLDRFRRGKRKLIDLCEHY
                     GVTVDAQALHAADADVLATLDLVQAMARAYPTLATADLSDLHDQQVSAHRTWAVEFRA
                     WLVSRGRTEDLPDLDWPLPLR"
     gene            complement(2038..3549)
                     /gene="guaB"
                     /locus_tag="VF362_02840"
     CDS             complement(2038..3549)
                     /gene="guaB"
                     /locus_tag="VF362_02840"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX7589895.1"
                     /translation="MAPDSEHDPFGLTGLTYDDVLLLPGESDVIPSEVDTSTRLTRGV
                     RLAVPLLSAAMDTVTESRMAIAMARHGGIGVLHRNLSVADHAHQVDLVKRSESGMITD
                     PVTVSPDATLAELDALCGQYRVSGLPVVDADRTLLGIITNRDLRFISPSDFAGRTVRE
                     TMTPMPLVTAPVGISNADAAALLGRHKVEKLPLIDANGRLAGLITVKDFVKTEKYPKA
                     TKDSEGRLRVAAAIGFYGDAWERATALVESGVDVLVVDTAHGHARAMIDMILRLKKDP
                     ATRGIEVIGGNVATRAGAQALVDAGVDAVKVGVGPGSICTTRVVAGVGVPQVTAVYEA
                     SRACGPAGVPVIADGGLQYSGDIAKALVAGAESVMIGSLLAGCEETPGDLVLVNGKQF
                     KTYRGMGSMGAMASRGRVSYSKDRYFQADAPSDDRIIPEGIEGQVPYKGPLSTVAYQL
                     VGGLGQSMFYVGAHTIPELRERGRFVRITPAGLRESHPHDVQITVEAPNYSPT"
     gene            3765..4058
                     /locus_tag="VF362_02845"
     CDS             3765..4058
                     /locus_tag="VF362_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX7589896.1"
                     /translation="MTEIVVLLPPPTQDEWEWQYGGACRDADPNLFFHPEGERGSARR
                     RRAESAKRFCGRCAVIEMCKERSLVAREPFGVWGGLSEDERNALLAGRVLQRN"
     gene            complement(4128..4421)
                     /gene="groES"
                     /locus_tag="VF362_02850"
     CDS             complement(4128..4421)
                     /gene="groES"
                     /locus_tag="VF362_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX7589897.1"
                     /translation="MSVSIKPLEDKVVVKPAAAEQTTASGLVIPDTVKEKPQEGEVMA
                     VGPGRVDDNGNRVPLDVAVGDKVIYSKYGGTEIKYAGEDYLIISSRDLLAVLA"
     gene            4614..>5086
                     /locus_tag="VF362_02855"
     CDS             4614..>5086
                     /locus_tag="VF362_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX7589898.1"
                     /translation="MDTDEARAATSPEGLALLSDLPPYTADGALNRGASLRAAGVAPS
                     LAAAVMTQARLRERARAKFGDFADGMLFTPEGLEQATRLTVAALHAERFRSAGIGRVA
                     DLTAGIGADAMAMSAIGIAVMAFETNEATALIADHNLRHWKQSVVVHADSLATVR"
BASE COUNT          764 a         1760 c         1765 g          797 t
ORIGIN      
        1 ggtggctaga gatcgccgac gcacggggcg gtggctagag atcgccgacg cacggggcgg
       61 tggctagaga tcgccgacgc acggggcgcc aaggcgccgc accgctagtg gggctcgtac
      121 ggcgagatga cgacgtcgac gcgctggaac tccttgaggt ccgaatagcc ggtggtcgcc
      181 aacgagcgcc ggagggcccc cataaggttc gtggtgccgt cggcgcgcga gcccggaccg
      241 aagacgatct cggccaaggg gccgaccgtg ccgacgtaca cgcgttcgcc ccggggaagt
      301 tcggcgtggt gagcctccgg gccccagtga tagccgcccc ctggcgcctc ggccgcccga
      361 gcgagcgcgg cgcccaccat gatggcgtcc gccccgcacg cgatggcctt gaccatgtcg
      421 cccgaccgac cgagcccgcc gtcggcgatc acgtggacgt accgtccgcc cgattcgtcg
      481 aggtagtcgc gccgcgccgc ggcgacatcc gcgacggccg tggccatcgg cgcgtggatc
      541 cctaccgtcg tccgcgtcgt gtgggcggct ccgccgccga atccgaccag cacgcctgcg
      601 gcgccggtcc tcatgaggtg gagcgcggcc tggtaggtcg aggctcctcc gacgatgacg
      661 ggaacgtcga ggtcgtagat gaactccttg aggttgagcg actccgcctc gcccgacaca
      721 tgctcggcgg acacggtcgt gcctcgaatg acgaagacgt cgaccccggc ggcgagaacg
      781 gtcttgtaga actcctgggt gcggtgcggg ctgagggccc cggccaccgt cacccccgcg
      841 gcgcgaatct cgccgagcct gagcttgatg aggtcggcgt cgaccggcgt gcggtagatc
      901 tcctgcatac gcctggtggc cacgtccgac cctagggagg cgatctcgat gaggtagggc
      961 gtcgggtcct cgtagcgtgt ccagaggccc tcgaggtcga ggacgccgag cccgccgagc
     1021 ttcccgagcg cgattgcggt cgcgggcgac atgacggagt ccatcggcgc cgcgatgatc
     1081 gggatctcga actggaaggc gtcgatttgc caccgaaggc tgacctgctc ggggtcgcgg
     1141 gttcgcctcg agggaaccac cgcgatgtcg tcgaaggcgt aggctctgcg gcctcgcttg
     1201 ccgcgtccga tctcgatgtc cgtgctcacg gccccagcct agcggcatgc ctctcggccg
     1261 ctgtctgcgg aggatgggag aatggggtca tgagttggct cgagcggacg atggtgggct
     1321 tcgacacgga gacaaccggc gtcgattccg ccaccgatcg gatcgtgacg gcggcagtca
     1381 tcttccggga tggcgccgac ggcgcggttt ccgcccacac atggctcatc gacccggggg
     1441 tcgagatccc cgagcgggct actgcggtgc acggaattac cacggagatg gcccgggcga
     1501 atggcgccga tccggtggtt gcgctcgggg agatcgcggc tcttctggcc gccgcactcg
     1561 ccaacggggc gccgatcgtc gccttcaacg cggggtacga cctcgggatc ctcgacgcgg
     1621 aactcgcgcg ccacggcctc acctcgctcg caaggcgtat cggcgcggca cacaccctcc
     1681 gcccggtggt cgatccgctg gtccttgacc gccacctcga ccggttccgc cgcggcaagc
     1741 gcaagctcat tgacctgtgc gagcactacg gggtcaccgt cgacgcgcag gcgctccacg
     1801 ccgccgacgc cgacgtgctc gccactctcg acctcgtcca ggccatggcg cgcgcctacc
     1861 ccacgctcgc gacggcggac ctctccgatc tccacgatca gcaggtgagc gcccaccgca
     1921 cctgggcggt ggagttcagg gcatggctcg tgtccagggg ccggaccgag gatcttcccg
     1981 acctcgactg gccgcttccg ctgcgctgag ggcgtcgacg cttgggtccc cggcgggcta
     2041 ggtcggggag tagttgggag cctcgacggt aatctgaacg tcgtgggggt gggactccct
     2101 gaggcccgcc ggcgtgatcc tcacgaagcg acccctctcc ctcagttccg ggatcgtgtg
     2161 cgccccgacg tagaacatgc tctggccgag gccccccacg agctggtacg ccacggtaga
     2221 gagcgggccc ttgtacggaa cctgcccctc gatgccctcg gggatgatgc gatcgtcgct
     2281 cggagcgtcc gcctggaagt agcggtcctt ggagtacgag acgcgcccgc gcgacgccat
     2341 ggcgcccatc gagcccatgc cgcgatacgt cttgaactgc ttgccgttca cgaggacgag
     2401 gtcgccgggg gtctcctcgc agccggcgag cagcgagccg atcatgaccg actccgcccc
     2461 cgcgacgagt gccttcgcga tgtcccccga gtactggagg cccccgtcgg cgatcacggg
     2521 cacgcccgcc gggccgcacg ccctcgacgc ctcgtagacc gccgtgacct gcgggacgcc
     2581 cacgcccgcg accaccctcg tggtgcagat cgatccggga cccacgccga ccttcacggc
     2641 gtccaccccg gcgtccacga gggcctgggc tcccgcacgg gtggcgacgt ttccgccgat
     2701 gacctcgatg cccctcgttg cgggatcctt cttgagcctc aggatcatgt cgatcatggc
     2761 gcgcgcgtgc ccgtgggcgg tgtcgaccac caggacgtcg acccccgact cgacgagcgc
     2821 ggtcgcgcgc tcccacgcgt cgccgtagaa cccgatcgct gccgcgaccc tgaggcgacc
     2881 ctcggagtcc ttggtcgcct tgggatactt ctccgtcttg acgaagtcct tgacggtgat
     2941 gagccccgcg agacgcccgt tggcgtcgat cagcggcagt ttctcgacct tgtgcctgcc
     3001 gagaagcgcc gcggcgtcgg cattggagat ccccacgggg gcggtgacga gcggcatcgg
     3061 cgtcatggtc tcgcggacgg tgcggcccgc gaagtccgag ggactgatga agcgaaggtc
     3121 gcggttcgtg atgatgccga ggagggtgcg gtcggcgtcc acgacgggca accccgagac
     3181 gcggtactga ccacacagcg cgtcgagctc cgcgagggtg gcgtcgggac tcacggtcac
     3241 ggggtcggtg atcatcccgg attccgaacg cttcaccagg tcaacctggt gggcatgatc
     3301 cgccacggag aggttccggt ggaggacgcc gatcccgccg tggcgcgcca tcgcgatcgc
     3361 catccgagac tccgtgaccg tgtccatcgc ggcgctgagc agcggcaccg cgagccgtac
     3421 cccgcgggtc agcctcgtcg aggtgtccac ctcggagggg atgacgtcgc tctcgcctgg
     3481 aaggaggagc acgtcgtcat aggtcaagcc ggtgagaccg aacggatcat gttccgaatc
     3541 tggcgccatt tcccgattct agtggacgcg ctcgtcgccc gaggccgggc ggaatgcccg
     3601 ggttgtctgc ccccacaagt gggggctacc ggcgcggggc ccagggggct acaaagggag
     3661 tcaggtaccc gccgtcggac gggaaggctc ctcacggagt cggggggcgg cggggaaccg
     3721 atcttccggg ccaccactga ggggcctcta cggctggggt tcacgtgaca gaaatcgttg
     3781 tgctgttgcc ccctcccact caggacgagt gggagtggca gtacggcggc gcgtgccgcg
     3841 acgcggaccc caacctcttc ttccaccccg agggcgagcg cggctctgcg cgccgtcggc
     3901 gggccgagtc ggccaagcga ttctgcgggc gctgcgcggt tattgagatg tgcaaggaac
     3961 gctcgctcgt cgcccgcgag cccttcggtg tctggggcgg gctcagcgag gatgagcgca
     4021 acgccctcct cgccggacga gtgcttcaac gaaactagcc acggggtacg aagagggacc
     4081 ccggacaatc tccggggccc ctcttcgtgc gtatgacgcg atgtcgccta ggcgagcacc
     4141 gcaagcaggt cgcgcgacga gatgatcagg tagtcctcgc cggcgtactt gatctcggtt
     4201 ccgccgtact tgctgtagat gaccttgtca cccaccgcaa cgtcgagcgg aacacggttg
     4261 ccgttgtcgt cgacccgacc ggggccgacc gccatgacct cgccctcttg gggcttctcc
     4321 ttgacggtat ccgggataac gaggccggag gccgtcgtct gctcggcagc ggccggcttc
     4381 acgacgacct tgtcttccag gggcttgatt gagaccgaca ctgcggacct caccttcctc
     4441 tacacgttcc gaacgatgag cggcggcgtc ggggcgaagc ctccgtcgcg gggtttgggc
     4501 ccgccgccga tggccgcacg cgctccttgt cggagcagta caaacttagc actctccctg
     4561 ggcgagtgct aactctccgc cgaggtgagg cccgcgcgtg gaatgatcgg cgcgtggaca
     4621 ccgacgaggc gcgcgcggcg acaagccccg aggggctcgc ccttctctcc gacctgcccc
     4681 cgtacacggc ggacggcgcg ctcaaccgag gggcgtcctt gcgcgcggcg ggcgttgcgc
     4741 cgagcctcgc cgctgcggtg atgacccagg cccggctccg cgagcgagcc cgcgccaagt
     4801 tcggcgactt cgccgacggg atgctcttca cccccgaggg gctcgagcag gcgacccgcc
     4861 tcacggttgc cgcgcttcat gccgaacggt tccgttccgc ggggataggg cgcgtcgccg
     4921 atctcaccgc aggaatcggc gccgacgcga tggcgatgtc ggcaatcggc atcgcggtca
     4981 tggccttcga gaccaacgag gcgaccgccc tgatcgccga ccacaacctg cgccactgga
     5041 aacaaagcgt cgtggtgcat gccgactcgc tcgcgaccgt gcgcga
//
LOCUS       DASVKP010000140         6160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_790277, whole genome shotgun sequence.
ACCESSION   DASVKP010000140 DASVKP010000000
VERSION     DASVKP010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790277"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VF362_02860"
     CDS             <1..141
                     /locus_tag="VF362_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX7589899.1"
                     /translation="MGHAGAIVSGSSGTAEAKRVALEAAGVRVGTTPSETARLVREIL
                     LG"
     gene            211..1497
                     /locus_tag="VF362_02865"
     CDS             211..1497
                     /locus_tag="VF362_02865"
                     /inference="COORDINATES: protein motif:HMM:NF040070.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6350 family protein"
                     /protein_id="HEX7589900.1"
                     /translation="MARTFRSDLRALAARAGTTVPGWVGGAMAGLQSALLSVGAIVAV
                     ALAVAAASAPADGTAGIDWEAAAAAGVRVWLLAHGVAAGTATASVSLIPLGLTAVFAS
                     LAASLARRFAVPTTGSWFAFTATYATCAALIASASDAAATAPHAVVRAALVGALVGGG
                     GAGAGLRRAGWLRGAAAGRFPVWLAAGLRLGVATALGLWAAGAIAFCFWTIAGWAQIA
                     QVAGTLDADPVGTLALSLGEAAYAPTLAVWGVAWLAGPGFSVGAGTEYAPAHLTTVPL
                     PSLPLLGALPHGAGGLLVLAPLVVIAIAAGTRALARRLAEQPSARAQVVAVPTVVVLA
                     AMGAMASRGSLGGGLLASVGPSPVATAIWVGVLVALGFAGGGLAARLTAPSSPKPTSP
                     RSPKARETGRRKPRNDAPARSPRPETPGRTRSSPPP"
     gene            1866..2507
                     /gene="purN"
                     /locus_tag="VF362_02870"
     CDS             1866..2507
                     /gene="purN"
                     /locus_tag="VF362_02870"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX7589901.1"
                     /translation="MTSDSPPASIVVLISGAGTTMSAIIEAAKEPGYGARVAAVISDR
                     AAAPGLALARGANVPTAVVALAEFPSREEWNRGITRAIDVFSPDLVVLAGFMKILSPA
                     TVAHFSGRVINTHPALLPSFPGAHGVRDALAHGVTVTGCTVILVDDGVDTGPIVAQAT
                     VDVLPGDSEETLHERIKTIERALVVDTLHALVTRGWTVEGRRVLLGGSGKDES"
     gene            2504..4063
                     /gene="purH"
                     /locus_tag="VF362_02875"
     CDS             2504..4063
                     /gene="purH"
                     /locus_tag="VF362_02875"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX7589902.1"
                     /translation="MNDRQRVRRALVSVYDKTGLEELARGLAEAGVELVSTGSTAARI
                     AAAGVPVTKVEDVTEFPECLDGRVKTLHPRIHAGILADRRLESHVSQLEAMGIAPFDL
                     VVSNLYPFAETVRSGAAPDEVIEQIDIGGPSMVRAAAKNHASVAVIVNPAEYGPALAA
                     IAAGGFTLAERQALAVEAFRHTATYDIHVASWMGTVISADDEGTGFPGWVGMSAKRRT
                     ALRYGENPHQAAALYVDQASEGGIANAEQLHGKEMSYNNYVDADAAWRAAHDHDGITV
                     AIIKHANPCGIAVGSSVADAHAKAHACDPVSAYGGVIATNREVTADAARQIAEIFTEV
                     VAAPSYEPEALEILTAKKNLRLLVVSGGRGPGEFRPISGGLLFQQADRIDAPGDDPAT
                     WTLATGPKASPEVLADLAFAWRACRSTKSNAILLAKGGASVGIGMGQVNRVDSCRLAV
                     ARAGAERASGSAAASDAYFPFADGFQVLADAGVIAVVEPGGSIRDAEVIAAAEAAGVS
                     LYLTGTRHFFH"
     gene            4073..4327
                     /locus_tag="VF362_02880"
     CDS             4073..4327
                     /locus_tag="VF362_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589903.1"
                     /translation="MTPRSRVPVWLPLLAVLLGVAVMMYATVVVGMWILAGLAMAEAT
                     ARGLGWEGATVRVRSRGIDIAVLASFAILLALLASSGVLD"
     gene            complement(4398..5345)
                     /locus_tag="VF362_02885"
     CDS             complement(4398..5345)
                     /locus_tag="VF362_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX7589904.1"
                     /translation="MAKNAPQSNPAEIMPIIEIDTWVVDESAGPTFVGRLFAEFLGTF
                     LFILIGLGVAVFGFVSSASTTLRGNTDITSLTGSFGGLNYAASLGNALAWAVALLALI
                     VAVGRISGAHFNPAVTLGMWIGGRMPGRDVAPYVVVQVLGALLAGTVVRTLVAGFPLF
                     TTGSDLIAASGGAAPTVGQGMSLITIGGGVHSSTGVSIAYAAIVEFIATAILVAVVLA
                     ATSRRAPKGQAPFTIGLAFAVLFLWAAPFSGGGLNPARITATALFASDAAGGHWAIGQ
                     LWIWWVVALLAGAFVGLLFRGFGPVEDLRALEATEAPEL"
     gene            5505..>6160
                     /locus_tag="VF362_02890"
     CDS             5505..>6160
                     /locus_tag="VF362_02890"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX7589905.1"
                     /translation="MGKIKVAGPVIELDGDEMTRIIWQLIRNRLILPHLDVDLHYYDL
                     SIENRDATDDQVTIDAAHAIREHGVGVKCATITPDEARVEEFGLKKMWKSPNGTIRNI
                     LGGVVFREPIVISNIPRLVPGWTKPIVIGRHAHGDQYKATDFKVPGAGSVTLTYTPAD
                     GSAPVEMEVVNMPEGGGVVMGMYNFNESIADFARASLRYGLDRGFPVYLSTKNTVLKA
                     "
BASE COUNT          942 a         2141 c         2156 g          921 t
ORIGIN      
        1 atgggccacg cgggtgccat cgtttccggc tcctccggca ccgccgaggc taagcgcgtc
       61 gcgctcgagg ccgcgggcgt ccgggtcggc acgactccgt ccgagaccgc ccgcctcgtg
      121 cgagagatcc tgctcggctg aggcggacga cgtcgaaccg ctggcggttc ggtcccctcc
      181 gagcggtgtc cggcgagcga cgatagggcc gtggcccgca cgtttcgctc cgacctacgc
      241 gcgctggcgg cgcgagccgg gaccaccgtg cccgggtggg tcggcggcgc catggccggc
      301 ctccagtcgg ccctcctctc ggtgggcgct atcgtcgccg tcgcgctcgc cgtcgccgcg
      361 gcctcggccc cggccgacgg aacggctggc atcgactggg aggccgccgc ggccgcaggc
      421 gttcgcgtct ggctccttgc ccacggcgtc gcggcgggca ccgcgaccgc atcggtcagc
      481 ctgatccccc tcggtctgac ggcggtcttc gcctccctgg ccgcgtccct tgcgcgccga
      541 ttcgcggtcc cgacgacggg gagttggttc gccttcaccg cgacatacgc gacgtgcgcc
      601 gcgttgatcg cctcggcgtc cgacgccgcc gcgaccgcgc cgcatgccgt ggttcgggcg
      661 gccctcgtcg gggcgctcgt ggggggcggg ggagcgggtg ctggactgcg gcgggcggga
      721 tggttgcgcg gcgcggccgc gggccgcttt ccagtgtggc tcgccgccgg cctgaggctc
      781 ggcgtggcga ccgccctcgg cctctgggcc gcgggtgcga tcgccttctg cttctggacg
      841 attgcgggat gggcccagat cgctcaggtc gccgggacgc tcgacgcgga tcccgtcggc
      901 acgctcgcgc tgtcgctcgg cgaggcggcc tacgctccga cgctcgccgt gtggggcgtc
      961 gcgtggctcg cggggcccgg attctccgtc ggcgcaggga ccgagtacgc gcccgcgcac
     1021 ctgacgacgg tgccgctgcc gtccctcccg cttcttggtg cgctccccca cggtgcgggc
     1081 gggctgctgg tactcgcacc gctggtggtc atcgcgatcg cggcggggac ccgcgcgctc
     1141 gcccggcggc tcgccgagca gccgagcgcc cgcgcacagg tggtagcggt cccgacggtg
     1201 gtggttctcg ccgcgatggg ggccatggcc tcgcgtggct cgctgggagg cgggctactc
     1261 gcgagcgtcg gcccgtctcc ggtcgcgacg gcgatctggg tgggcgttct cgtggcgctg
     1321 ggattcgccg ggggaggtct cgcggcgagg ctgacggccc ctagttcgcc gaagccgacg
     1381 tcgcctcgct cgccgaaggc gagggagacg ggtagacgga aaccccgaaa cgatgcgccc
     1441 gcgcgttcac cgcgtcccga aactcccggt cgcacgcggt cgtcgccccc accgtgatcg
     1501 cgccgctccg gcactcctgg taggcctcga actcccgata gaacacggcc tcggctgcgt
     1561 aggtcagcag gagccccccg cacaggagga ggcccgccgc catcactacc cacagcatgg
     1621 tgctcgcggt gcgccgcatc ttgaccatcg cgacgatcgc ggtaaccgag gccgccacgg
     1681 ccgcagggat gccgacaagg ctccacggca gtgggagcat cgtcgtgacg acggcgatcg
     1741 cgaggaagta ggcgaaccat cgtacgaagc gcagcgcggg gagggaaagg cgcggcgggg
     1801 ttggggcggg gttcacgggg atccttgcgt aggtgagatg accagcatgc ctatagggtc
     1861 gggatgtgac aagcgactcg ccccctgcct cgatcgtggt cttgatctcg ggcgcgggaa
     1921 ccacgatgtc ggcgatcatc gaagccgcga aggaaccagg ctacggcgcg cgggttgccg
     1981 cggtcatttc ggatcgagcg gcggcgccgg ggctcgccct cgcccgcggc gcgaacgtgc
     2041 cgaccgccgt tgtggcgctc gcggagtttc cgagtcggga ggagtggaat cgcggcatca
     2101 ctagggccat cgacgtcttc tcccccgacc tggtcgtcct cgcgggcttc atgaagattc
     2161 tctctccggc gaccgtggcc cacttctcag gtcgcgtcat caacactcat ccggcgctcc
     2221 tgccatcctt ccccggtgcc cacggggtcc gcgacgcgct agctcacggg gtcacggtga
     2281 cggggtgcac ggtgatcctc gtcgacgacg gcgtggacac cggaccgatt gtcgcccagg
     2341 cgacggtaga cgtcctcccg ggcgactcgg aggagacgct tcacgaacgg atcaagacca
     2401 tcgagcgtgc gctcgtcgtc gacaccctcc acgcgctcgt gacgcgcgga tggacggtcg
     2461 agggacgccg cgtcttgctc ggaggctcag gaaaggacga atcatgaacg accgtcaacg
     2521 ggtccgcagg gccctcgtat ccgtgtacga caagaccggc ctcgaggaac tggcgcgggg
     2581 gctcgcggaa gctggcgtcg agctcgtctc gacgggaagc actgcggcgc gcatcgcagc
     2641 cgctggcgtt ccggtcacca aggtggagga cgtcacggag tttcccgagt gcctggatgg
     2701 cagggtcaag acccttcacc cgaggatcca tgcgggaatc ctcgcggacc gcaggctcga
     2761 gtcccacgtg agccaactcg aggcgatggg catcgccccg ttcgacctgg tggtgagcaa
     2821 cctctacccg tttgccgaga ccgttcgctc gggcgccgcg ccagacgagg tcatcgagca
     2881 gatcgacatc ggcggtccgt cgatggttcg cgccgcggcg aagaatcacg cgagcgtcgc
     2941 ggtcatcgtc aacccggccg agtacggccc cgcgctcgcc gcgattgccg cgggaggctt
     3001 cacgctcgcc gagcgacagg ccctcgcggt tgaggcgttc cggcacacgg ccacctacga
     3061 catccacgtg gcctcgtgga tgggaacggt gatctccgcg gacgacgagg ggaccggctt
     3121 ccccggctgg gtggggatga gcgcgaagcg ccgaaccgcg cttcgctacg gagagaaccc
     3181 ccatcaggcc gcggccctgt acgtcgacca ggcgtccgag gggggcattg cgaacgccga
     3241 gcagcttcac ggcaaggaga tgtcgtacaa caactacgtc gacgccgacg cagcatggcg
     3301 cgcggcgcac gatcacgacg ggatcaccgt cgcgatcatc aaacacgcga acccgtgcgg
     3361 catcgccgtg ggctcaagcg tcgcggacgc ccacgccaag gcccacgcgt gcgacccggt
     3421 ctcggcctat ggaggcgtga tcgccaccaa ccgcgaggtg acggccgacg ccgcgcgcca
     3481 gatcgccgag atattcaccg aggtcgtcgc ggctccaagt tacgagcccg aggccctcga
     3541 gatactcacc gccaagaaga accttcggct actcgtcgtc tccggcggtc gagggccggg
     3601 tgagttccgc cccatctcgg ggggcctcct cttccagcag gccgatcgga tcgatgcacc
     3661 cggcgacgac cccgccacgt ggacgctcgc gacggggccg aaggcctcac cggaggtgct
     3721 cgccgacctc gcgttcgcct ggcgcgcgtg ccgctccacc aagtccaacg cgatcctgct
     3781 cgccaagggt ggcgcgtcgg tggggattgg catggggcag gtaaaccgcg tcgactcgtg
     3841 ccgcctggcg gtcgcccgcg cgggcgccga gcgcgcctcg ggttcggccg cggcctccga
     3901 cgcatacttt ccgttcgccg acggcttcca ggttctggcc gatgccggcg tgatcgccgt
     3961 cgtcgagccg ggcggctcga tccgggacgc cgaggttatc gccgccgcgg aggccgcagg
     4021 tgtctccctg tacctcaccg gcacccggca cttcttccac taggtccacc cggtgacccc
     4081 ccgctcgcgc gtccccgtgt ggttgccact cctcgccgtc ctcctcggcg ttgcggtgat
     4141 gatgtacgcc acggtggtcg tgggtatgtg gattctcgcg ggcctcgcca tggcggaagc
     4201 gaccgcccga gggctgggct gggaaggggc aaccgtgcgc gttcggagcc ggggtatcga
     4261 catcgcggtc ctcgcgtcct tcgccatcct ccttgccctc cttgcctcct ccggggtact
     4321 cgactagccg cagccccaga gcgcgagagg ggcccggtcg gcttgcgccg accgggcccc
     4381 tctcggtgaa ggtacggcta cagctccgga gcctcggtcg cttcgagcgc gcgcagatcc
     4441 tcgaccggac caaagccgcg gaagagcagg cccacgaagg cgccagcgag gagggcgacg
     4501 acccaccaga tccacaactg accgatcgcc cagtggccgc ccgccgcgtc ggacgcgaag
     4561 agcgccgtgg cggtgatgcg cgcggggttg aggcccccgc ccgagaacgg cgccgcccac
     4621 aagaacagga ccgcgaacgc aagcccgatc gtgaatggag cctggccctt cggggccctc
     4681 cgcgacgttg cggcgagcac cactgcaacg agaatcgccg tcgctatgaa ctcaacgatc
     4741 gcggcgtacg cgatgctgac gcccgtggag gagtggacgc cacctccgat agtgatgagc
     4801 gacatgccct gccccaccgt cggggcggct ccgcccgacg cggcgatgag gtccgagccg
     4861 gtcgtgaaca gcgggaatcc cgcaacgagg gtgcggacaa ccgttcccgc gagcagcgcg
     4921 cccaggacct ggacgacgac gtatggagcg acatcgcggc cgggcatgcg cccgccgatc
     4981 cacataccga gggtgacggc ggggttgaag tgcgctccgg agatgcggcc gacggcgacg
     5041 atgagcgcga gcagcgcaac cgcccaagcg agcgcatttc ccagggacgc ggcgtagttg
     5101 agtccgccga aggagcccgt gagcgaggtg atatcggtgt tgccgcgaag ggtcgtcgaa
     5161 gccgaggaaa cgaacccgaa cacggcgacg ccgaggccga tgagtatgaa caggaaggtc
     5221 ccgaggaatt ccgcgaagag gcgtccgacg aaggtggggc cagccgactc gtcaacaacc
     5281 cacgtgtcaa tctcgatgat gggcatgatc tccgcagggt tcgactgcgg cgcattcttg
     5341 gccatggtgg tccgatctgt gatggggaag cggtcatcgc ttctccgcac atcctgccac
     5401 gccgtacgtg ggcgcggtgt agacggatcc gcgcgctcac ggcccctcgc ctccgtgagt
     5461 tagcctgggc agtgcacccg tcgcgtagaa cgaggagaag ttgcatgggc aagatcaagg
     5521 tcgccggtcc cgtcatcgaa ctcgacggag acgagatgac gcgcatcatc tggcaactga
     5581 tccggaacag gctgatcctt ccccatctcg acgttgacct gcactactac gacctctcga
     5641 tcgagaaccg cgatgctacc gacgaccagg tgacgattga cgccgcccac gcgatcaggg
     5701 agcacggagt cggcgtcaag tgcgcgacga tcaccccgga cgaggctcgc gtcgaggagt
     5761 tcggcctcaa gaagatgtgg aagtcgccga acgggacgat ccgcaacatc ctcggcggcg
     5821 tcgtcttccg cgaacccatc gtcatctcga acatcccgcg cctcgtgccc ggctggacca
     5881 agccgatcgt gatcgggcgg cacgcccacg gcgaccagta caaggcgacg gacttcaagg
     5941 ttcccggcgc cggaagcgtg accctgacgt acaccccggc cgacggctcc gcgccggtgg
     6001 agatggaggt ggtgaatatg cccgagggtg gcggcgttgt catgggcatg tacaacttca
     6061 acgaatccat tgccgacttc gcccgcgcct cgctccgcta cgggcttgac cgcggcttcc
     6121 cggtctacct ctccacgaag aacaccgtcc tcaaggccta
//
LOCUS       DASVKP010000141         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_377684, whole genome shotgun sequence.
ACCESSION   DASVKP010000141 DASVKP010000000
VERSION     DASVKP010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377684"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VF362_02895"
     CDS             complement(<1..535)
                     /locus_tag="VF362_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589906.1"
                     /translation="MAGCEIVDVGPIEAWGEHFGGFSPATSLHGRRLLDKELPLEFFG
                     ITVNSRLPGEGADYRHAHSVLEELYLFLAGDGVMLLGDKVYPVGPGTAIRVGQGVMRS
                     WYCLPESPAPLAWLCVRAGGAPLSAIPSDAEPLRDVRLPWAPGQPPLTIQPADAPLPE
                     GCQRYEAGARAAWGGAYG"
     gene            complement(739..1665)
                     /locus_tag="VF362_02900"
     CDS             complement(739..1665)
                     /locus_tag="VF362_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589907.1"
                     /translation="MRAKVATPDSHDFPTRPYDLVKEGAIAFGVILVLALALAAVFSS
                     PDEKAISFKAWASATPNDVVVTAVSELAGTSGSAGYGAPYNTNAEGQSFGPLALQRWG
                     GVTVPIDSANDLVIGPLGSVQGDTALSGALAQWKAATADQQSAWATAYADALDAAGGD
                     PAAVADGAYGPVPELGSAFLTLAQSGGLEQLLAGGFYGGDQTKALLLLSDGTYLEDQA
                     VARSLGGDQWGMINSTGNNPGQPWLAPVSFWYQIEPFKSSENADAIIFGMMGLVVLVV
                     ILLPFIPGLRAIPERIPVYRLVWRDFYRNQKK"
     gene            complement(1662..>2352)
                     /locus_tag="VF362_02905"
     CDS             complement(1662..>2352)
                     /locus_tag="VF362_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX7589908.1"
                     /translation="PQGARRRRGRGQEVTATTIKAPSSPQETEGSWTVILRKRIVGLV
                     PPGQALPDRQPAYMQSWIYVFGMATLASLGVVILSGIGLSMGGTVWWHTSALGHYVNS
                     VHLWSVEIFFVVLVVHLWGKFWMAAWRGKRALTWMTGMFAFLVAVGTAFTGYLTTTNF
                     ESQWIAAEAKDGLNSIGVGGFVNALNPARMELWHVVLLPLVVGLIVVGHLILVRRHGI
                     VPPIGAKEPKK"
BASE COUNT          386 a          806 c          813 g          347 t
ORIGIN      
        1 ccccgtaggc gcctccccac gcggcgcgag cccccgcctc gtaccgctgg caaccctccg
       61 ggaggggtgc gtcggcgggc tgaatcgtca gcgggggctg gcccggcgcc caggggagcc
      121 gtacgtcgcg gagcggttcg gcgtccgacg ggatggccga gagcggcgct ccgcccgcgc
      181 ggacgcacaa ccaggcaagc ggcgcggggc tctcgggcag gcagtaccaa ctccgcatga
      241 cgccctggcc gacgcgaatg gccgtgcccg gcccgaccgg gtagaccttg tcgccgagca
      301 gcatgacgcc gtctcccgcg aggaacaggt acaactcctc gaggacggag tgcgcgtggc
      361 ggtagtccgc gccctcgccg gggaggcgcg agttgacggt aatcccgaag aactcgagcg
      421 gcagttcctt gtcgagcagt cgtctgccgt gcagcgacgt cgcggggctg aatccgccaa
      481 agtgttcgcc ccacgcctca atggggccaa cgtccacgat ctcgcagccg gccatcgtcc
      541 cgcctctccg ctcgacgcac gtcactccgg agtcagccta gtccgccccc cgctggcgtc
      601 gcacgtcgaa gcgtcgcacg tcgaagcgtc gcgcatcgac ccgggtcacg cgctggcctc
      661 gggcactccc cgaacacaaa agcgcgggcg cccggccgaa gtggccgagc gcccgcacca
      721 gtgtgttcta ggaggaactc acttcttctg gttgcggtag aagtcccgcc acaccaaccg
      781 gtataccggg atcctctccg gaatggccct gagccccggg atgaagggca gcaggatcac
      841 gaccaagacg acgagcccca tcattccgaa gatgatggcg tccgcattct cggaggactt
      901 gaagggttcg atctggtacc agaacgacac aggggccagc cagggctggc cggggttgtt
      961 gcccgtcgag ttgatcattc cccactggtc gccgccgaga ctcctagcaa cggcctggtc
     1021 ctcgaggtag gttccgtccg acaggagcag gagcgccttg gtctggtcgc cgccgtagaa
     1081 gccgccagcc aggagttgct caagcccgcc cgattgcgcg agcgtgagga atgccgagcc
     1141 gagttccggt accggtccgt aggcgccgtc cgccaccgcg gcagggtcgc cgcccgcggc
     1201 gtcgagtgcg tcggcgtagg cggtcgccca tgcggactgc tggtccgcgg tcgccgcctt
     1261 ccattgcgcg agcgcgccgg agagggcggt atcgccttgg acgctcccga gcggcccgat
     1321 caccaggtcg ttcgcggagt cgatcggcac cgtcacgccg ccccatcgct ggagcgcgag
     1381 cggcccgaag gactggccct cggcgttcgt gttgtacggc gcgccgtacc ccgcgcttcc
     1441 cgacgttccg gcgagttccg agacggccgt cacgacgacg tcgttcgggg tcgccgaggc
     1501 ccaggccttg aaggagatcg ccttctcgtc cggcgaggag aagaccgccg cgagcgcgag
     1561 cgcgaggacg aggatcacgc caaacgcgat ggcgccctcc ttgacgaggt catacggccg
     1621 cgtcgggaag tcgtgcgaat ccggggttgc cactttcgct ctcatttctt gggctccttc
     1681 gcgccgatgg ggggcacgat gccgtggcgg cgaaccagga tgaggtgccc aacgacgatc
     1741 aggccgacga ctaggggcag aaggaccacg tgccagagct ccatgcgcgc ggggttaagc
     1801 gcgttgacga atccgccgac gccgatggag ttgagcccat ccttcgcctc ggcggcgatc
     1861 cactgggact cgaagttcgt cgtcgtcagg taccccgtga aggcggtgcc gacggcgacc
     1921 aggaatgcga acatgcccgt catccacgtg agcgcgcgct tgccgcgcca tgccgccatc
     1981 cagaacttgc cccacaggtg gacgacgagc acgacgaaga agatctcgac gctccacagg
     2041 tggacggagt tgacgtagtg gccgagggcc gaggtgtgcc accacaccgt tccgcccatc
     2101 gagaggccga tccccgagag gatgaccacg ccgagcgacg cgagggtcgc catgccgaag
     2161 acgtagatcc acgactgcat gtaggcgggc tggcggtccg ggagggcctg gcccggggga
     2221 acaagcccga cgatgcgctt gcggaggatg acggtccaac taccctcggt ctcctgaggc
     2281 gacgagggcg ccttgatggt cgtggcggtc acttcttgcc cccgccctcg gcgccgtcgt
     2341 gccccttggg ga
//
LOCUS       DASVKP010000142         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1367299, whole genome shotgun sequence.
ACCESSION   DASVKP010000142 DASVKP010000000
VERSION     DASVKP010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367299"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /gene="alr"
                     /locus_tag="VF362_02910"
     CDS             complement(<1..1020)
                     /gene="alr"
                     /locus_tag="VF362_02910"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX7589909.1"
                     /translation="MSGLTVAVDYGAITRNTRVLLGRAGGAGLMAVVKADGYGHGLVE
                     TARAARAGGASWLGVAQPEEALALREAGDTGRILAWLYGPDLPAAALIAADVDLSVSS
                     LSNLSAVLAGAKEAGSRARIHVCVDTGLGREGATPDDLPALLDAVERERAAGAIEAVG
                     IWSHLAWADVPGHPTVDDQAEVFRFALDLARERGLGFEVRHLANSAAALTRPDLRFDL
                     VRPGIALYGLSPLVEADGAGFDLTPAMSVTSVVALVKRVSAGHGVSYGHDYVTERETY
                     LGLVPAGYADGVFRSAGGRAEVSIRGERYRIAGRVCMDQFVVDLGPSTSVAEGDVVTL
                     IGPDGP"
     gene            complement(1017..1373)
                     /locus_tag="VF362_02915"
     CDS             complement(1017..1373)
                     /locus_tag="VF362_02915"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX7589910.1"
                     /translation="MIVGLGIDVVALDRFRETIEKTPGLVERVFTPAERTMGHASLAA
                     RWAAKEALAKALGAPVGLRWQDAEVVREESGAPTLVVTGTVAARAAELGVTRFHLSMS
                     HDGGMASAVVVAEADA"
     gene            complement(1376..>2550)
                     /gene="glmS"
                     /locus_tag="VF362_02920"
     CDS             complement(1376..>2550)
                     /gene="glmS"
                     /locus_tag="VF362_02920"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX7589911.1"
                     /translation="DASGAVVARAPYTIDWDATAADKGGFSTFMEKEIHDQPAAVADT
                     LRDRFDEFGKLKLDGGLIAESVLSSIDKIIVIACGTSSYAGLVAKYAIEHWCRIPVEV
                     EYSHEFRYRDPVVSVKTLIVAISQSGETMDTIMAMRYAQQQGASVVAIVNVQGSTIAR
                     EADAVLYTRAGPEIAVASTKALVAQITATYLLGMFLAELRGNKFPGEVRAILEQLGEM
                     PAKIQRVLDTAEPIRALARSMVGERTILFLGRHVGYPVALEGALKLKELAYIHAEGFA
                     AGELKHGPIALVEKDLPIFVVLPSPNGPDSLHAKVLSNIQEVRARGARTIVLAEEGDE
                     VAAKYASEIFYLPEAPVLLTPLLALIPLQIFACELAGAKGNDVDQPRNLAKSVTVE"
BASE COUNT          391 a          912 c          870 g          377 t
ORIGIN      
        1 cgggccgtcg gggccgatca gcgtcacgac gtcgccctcg gcgacgctcg tcgagggacc
       61 gaggtcgacg acgaattggt ccatgcagac ccgaccggcg atgcggtagc gctccccccg
      121 aatcgagacc tcggcgcgtc cgccggccga acggaagact ccgtccgcat atccggcggg
      181 aacgaggccg aggtaggtct cgcgctccgt gacgtagtcg tgcccgtagc tgaccccgtg
      241 gcccgcgctg accctcttca cgagggcaac gacgctcgtg accgacatcg cgggtgtcaa
      301 atcgaaaccc gcgccgtcgg cctcgacgag gggcgagaga ccatagagcg cgatcccggg
      361 ccggacgagg tcgaagcgca ggtcggggcg ggtgagcgcc gcagctgagt tcgccaggtg
      421 gcgcacctcg aagccgaggc cacgctcgcg ggcaaggtcc agggcgaatc ggaatacctc
      481 cgcttggtcg tcgaccgtcg ggtggcccgg cacgtccgcc cacgcgaggt gcgaccagat
      541 gccgaccgcc tcgatggcgc cggcggcgcg ctcgcgctca accgcatcga gaagcgccgg
      601 aaggtcatcg ggcgtcgcgc cctccctgcc gaggccggtg tcgacgcaca cgtggatcct
      661 cgcccgcgag ccagcctcct tggcccccgc gagcaccgcg ctcaggttgg acagcgacga
      721 cacggacagg tcgacgtcgg cggcgatcag ggccgccgcg ggcaggtccg gcccgtagag
      781 ccacgccagg atccgcccgg tgtcgccggc ctctcgcagc gcgagggcct cctccggttg
      841 cgccacgccg agccaggagg cgccacccgc cctcgccgcg cgagccgtct cgacgagccc
      901 gtgcccgtag ccgtccgcct tgaccaccgc catgagcccc gctcccccgg cacgcccgag
      961 caggacccgg gtgtttcgcg tgatcgcacc gtagtcgacg gcgaccgtca gcccgctcac
     1021 gcgtccgcct ctgcgacgac cacggccgag gccatgccac cgtcgtgcga catcgagagg
     1081 tggaagcgcg tgacgccgag ctccgcggcg cgcgcggcaa cggttccggt cacgaccagg
     1141 gtcggggcgc ccgactcctc gcgcactacc tcggcgtctt gccacctcag tcccacgggg
     1201 gcgccaagcg ccttcgcgag ggcctccttg gccgcccagc gcgccgcgag gcttgcatgc
     1261 cccatcgtcc gctccgcggg agtgaagacg cgctcgacga ggccgggggt cttctcgatc
     1321 gtctcccgga agcgatcgag cgcgaccacg tcgatgccaa ggccgacgat catcgctact
     1381 ccaccgtgac ggacttcgcg aggttgcgcg gctggtccac gtcgttgccc ttcgcgccag
     1441 caagctcgca cgcgaagatc tggagcggga tgagggccag gagcggcgtc aggaggacgg
     1501 gcgcctcggg caggtagaag atctcgctcg cgtacttcgc cgcgacctcg tctccctctt
     1561 ccgcgaggac gatggtgcgg gcgccccgcg cacgtacctc ctggatgttc gacagcacct
     1621 tggcgtgaag ggagtcgggc ccgttgggcg acggaagcac cacgaagatg gggaggtcct
     1681 tctcgaccag cgcgatcggc ccgtgcttga gttcgcccgc cgcgaatccc tcggcgtgga
     1741 tgtacgcgag ctccttgagc ttcagcgcgc cctcgagcgc gacggggtac ccgacgtggc
     1801 ggccgaggaa gaggatggtt cgctcgccca ccatcgagcg agcaagagcg cggatgggtt
     1861 ccgccgtgtc cagcacgcgc tggatcttgg cgggcatctc gccgagttgc tcgaggatcg
     1921 cgcgcacctc gccggggaac ttgttccccc gcagttcggc gaggaacatc ccgaggaggt
     1981 acgtcgccgt gatctgcgcg acgagggcct tggtagacgc caccgcgatt tccggccccg
     2041 cgcgcgtgta caagaccgcg tcggcctcgc gggcgatggt gctgccctgg acgttgacga
     2101 tggcgacgac gcttgccccc tgctgctgcg cgtaccgcat cgccatgatc gtgtccatcg
     2161 tttcgccgga ctgcgagatc gcgacgatga gggtcttcac tgagacaacc gggtcccgat
     2221 accggaactc gtgcgagtac tccacctcca ccgggatgcg gcaccagtgc tcgatcgcgt
     2281 acttggccac caggcccgcg tagctcgagg tcccgcaggc gatgacgatg atcttgtcga
     2341 tggaactcag aacgctctcg gcgatcagcc ccccatcaag tttgagtttc ccgaactcgt
     2401 cgaatcgatc gcgaagggtg tccgcgacgg ccgcaggctg gtcgtgaatc tccttctcca
     2461 tgaacgtgga gaagccgccc ttatccgcgg cggtcgcgtc ccaatcgatc gtgtagggcg
     2521 cgcgcgccac gacggcgccg ctcgcgtccg
//
LOCUS       DASVKP010000143         4263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_627342, whole genome shotgun sequence.
ACCESSION   DASVKP010000143 DASVKP010000000
VERSION     DASVKP010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627342"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..705
                     /locus_tag="VF362_02925"
     CDS             142..705
                     /locus_tag="VF362_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX7589912.1"
                     /translation="MNKTFPLVPKDWVPTDLVAMPGIPEGSDQKMRADAAAALTTLSE
                     AANAAGAGFKVSTAYRSYSFQKALYDPRAKSRGVAVADRTTARPGFSEHQTGLAVDIY
                     TTDACHLDPCFAATAAGQYVAEHAWEYGFILRYPEGKEGVTGYVSEPWHLRYVGKDLS
                     TAMHDSGVLTLEEFFGLPAAPDYAASP"
     gene            737..1576
                     /gene="rsmI"
                     /locus_tag="VF362_02930"
     CDS             737..1576
                     /gene="rsmI"
                     /locus_tag="VF362_02930"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX7589913.1"
                     /translation="MAGSIVLAATPIGNVGDASARLIDALATADVVAAEDTRRLRALA
                     ARLGVAVKGEVLSLNDHNERERAGDLVSRAAAGAMILVVSDAGMPTVSDPGFRVVVAA
                     AEAGVAVTVLPGPSAALAALAVSGLATDRFCFEGFLPRKAGERATALAALGAEPRTMV
                     FFEAPHRLAATLAAMAEAFGSARPATVSRELTKTYEETRRGGLAELAVWAHAAAPKGE
                     FVVVVAGAPKAGASFDALVSEALARAASGERLKEAVRDVSAVAGVSSRELYAAVLEAR
                     AAP"
     gene            1634..3427
                     /gene="metG"
                     /locus_tag="VF362_02935"
     CDS             1634..3427
                     /gene="metG"
                     /locus_tag="VF362_02935"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX7589914.1"
                     /translation="MSRILSAVAWPYANGPRHIGHVAGFGVPSDVFSRYMRMAGNDVL
                     MVSGTDEHGTPILVQAEQEGVPPLELADRYNRVIAEDLTALGLSYDLFTRTTTGNHFA
                     VAQAMFRAVHANGYMIEQTTRGAISPSTGRTLPDRYIEGTCPICGYDGARGDQCDNCG
                     NQLDAIDLKNPVSRINGETPKFVETEHFFLDLPALATQIGDWLGSRQGWRPNVLNFSL
                     NLLDDVRPRAMTRDIDWGIPVPLPGWEDNPAKRLYVWFDAVIGYLSASIEWARRTGDP
                     EAWRKWWNDPQALSYYFMGKDNITFHSQIWPGELLAANGRGAKGGHLSEFGDLQLPTE
                     VVASEFLTMESNKFSSSRGHVIYVRDMLSRYQPDALRYFIAVAGPETSDSDFTWAEFK
                     RRTNDELVAAWGNLVNRTVSLLHRNVGTIPPLTDLSDADRAALAQSAIGFETVGRLIG
                     RQRQRQAIGEAMRVVGDANKYLAEMEPWKLKATDPARMATVLGVAAQLVSDANTLLAP
                     FLPHSAQRIHEALGGQGTFAPMPRMEEVEDLDLPGRSYPIITGDYTGVRDTWARHDLV
                     AGTPIPAPVPVFTKLDDSIVDDELARMRGEE"
     gene            3482..>4263
                     /locus_tag="VF362_02940"
     CDS             3482..>4263
                     /locus_tag="VF362_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7589915.1"
                     /translation="MADNHAHLEWPAGDEPKTVAERLADAAAVGVDRVVQIGCEVASA
                     RWTALAVVQYPTLVGGVAIHPNEAPLHEAGTHESGLGYDEAFAIIAELARGPRMRVIG
                     ETGLDFYRTGPEGRDAQVGAFRDHIALAKELGLVLQIHDRDAHAEVVEVLDRDGAPDR
                     TVMHCFSGDAGLARLCVDRGWYLSFAGTLTFKNSHELRGALAVTPLDRVLVETDAPFL
                     APHPHRGAPNAPYLVPWTMREIAGVHAVALEEACAAVSATSDA"
BASE COUNT          645 a         1514 c         1476 g          628 t
ORIGIN      
        1 cctgcgggaa cgacgacccc gaccgccacc gcggcgccgt cggcctcggt gacggtcgcc
       61 gtgagcccaa ccccaagccc gtcccagacg ttcgacaagc tcgcgtttcc gctcgatgac
      121 ccgacctcga tttggctcat cgtgaacaag acgttcccgc tcgtgcccaa ggactgggtc
      181 ccgacggacc tcgtggcgat gccgggcatt cccgagggga gcgaccagaa gatgcgggcg
      241 gacgcggccg cggcgctgac gactctgtcc gaggcggcga acgcggcggg cgcgggtttc
      301 aaggtgtcaa ccgcttaccg gagctactcc ttccagaagg ccctctacga ccctcgggcc
      361 aagtcgcgcg gcgtcgctgt cgcggaccgg acgacggcgc ggccgggctt ctccgagcac
      421 cagaccggac tcgccgtcga catctacacc acggacgcct gccacctcga tccatgcttc
      481 gcagcgacgg ccgccgggca atacgttgcg gagcatgcct gggagtacgg gttcatcctt
      541 cgctaccccg agggcaagga gggcgtcacg ggctacgtct ctgaaccctg gcacctccgg
      601 tacgtcggga aggacctgtc gacggcgatg cacgactccg gagtgttgac cctcgaggaa
      661 ttcttcgggc tccccgccgc gccggactac gccgcctcgc cgtgaaccgc ccaccggggt
      721 gtgcgacgct agggccatgg caggaagcat cgtccttgcg gccactccca tcggcaacgt
      781 aggggatgcg tcggcgcgcc tgatcgacgc gcttgcgacg gccgacgtcg tggccgccga
      841 ggacacgagg cgccttcggg cccttgcggc acgcctcggc gtggcggtca agggtgaggt
      901 gctctcgctg aacgaccaca acgaacgcga gcgggcgggt gatctcgtgt cgcgcgccgc
      961 cgcgggggcc atgatcctgg tcgtgtccga cgcggggatg ccgacggtgt ccgatcccgg
     1021 cttcagggtc gtcgtcgcgg cggccgaggc gggcgtcgcc gtcaccgtcc ttcccggtcc
     1081 gagcgcggcg ctcgccgcgc tcgcggtgtc ggggctcgcc acggatcggt tctgcttcga
     1141 gggcttcctt ccgcggaagg cgggggagag ggccaccgcg ctcgcggcgc tgggcgccga
     1201 gccacgcacc atggtgttct tcgaggctcc ccaccgcctc gccgcgacgc tcgccgcgat
     1261 ggcggaggcc ttcggttcgg cgcggcccgc caccgtgagc agggagctca ccaagacgta
     1321 cgaggagacg aggcgcgggg gcctcgcgga gctcgcggtc tgggctcatg cggcggcgcc
     1381 caagggcgag ttcgtcgtgg tggtggccgg ggcgccgaag gcgggagcct ccttcgacgc
     1441 gctcgtttcc gaggcgctgg cgagggcagc atcgggggag cggctcaagg aggcggttcg
     1501 ggacgtctcg gcggttgctg gcgtttcgtc gcgcgagctg tacgccgcgg tgctcgaggc
     1561 gcgggccgcg ccctaggcgt gctcgaacgg gcgtggccct cgggtcccgc atcgtcgtca
     1621 gtaggatgtg accatgtccc gcatcctctc tgccgtcgcg tggccctacg ccaacggccc
     1681 ccgccacatc ggtcacgtcg cgggcttcgg agtgccgtcg gacgtgttca gtcgctacat
     1741 gcgcatggcg ggcaacgacg tcctgatggt ctcggggacc gacgagcacg ggacgccgat
     1801 cctggtgcag gccgagcagg agggcgtgcc gccgctcgaa ctcgcggacc gctacaacag
     1861 ggtcatcgcg gaggacctca cggcgctcgg cctgagttac gacctcttta cgcgcaccac
     1921 gacgggtaac cacttcgccg tcgcccaggc gatgttcagg gccgtccacg cgaacggcta
     1981 catgatcgag cagacgacgc ggggcgcgat cagcccctcc accggccgga cgctgcccga
     2041 ccgctacatc gagggcacgt gccccatctg cgggtatgac ggcgcgcgcg gcgaccagtg
     2101 cgacaactgc ggcaaccagc tcgacgcgat cgacctgaag aaccccgtga gccggatcaa
     2161 cggggagacg cccaagtttg tcgagaccga gcacttcttc ctggacctcc ccgcgctcgc
     2221 gacccagatc ggcgactggc tcggctcgcg ccagggatgg cgccccaacg tcctcaactt
     2281 ctcgctcaac ctcctggacg acgtgcggcc cagggcgatg acgcgcgaca tcgattgggg
     2341 catcccggtg ccgctgcccg gctgggagga caacccggcg aagcgcctct acgtgtggtt
     2401 cgacgccgtc atcggctacc tctcggcctc gatcgaatgg gcgcggcgga cgggcgaccc
     2461 cgaggcatgg cgcaagtggt ggaacgaccc gcaggccctc tcgtactact tcatggggaa
     2521 ggacaacatc accttccact cgcagatctg gccgggtgag ttgctcgcgg cgaacggtcg
     2581 gggcgcgaag ggtgggcatc tcagcgaatt cggcgacctg caacttccga ccgaggtcgt
     2641 ggcgagcgag ttcctcacca tggagtccaa caagttctcg tcctcgcggg gacacgtgat
     2701 ctacgtgcgc gacatgctct cgcgttacca gcccgacgcg cttcggtact tcatcgccgt
     2761 ggcgggaccg gagacgtcgg actccgactt cacgtgggcg gaattcaaac gccgcacgaa
     2821 cgacgaattg gtggccgcgt gggggaatct cgtcaatcgc acggtgtccc tcctccaccg
     2881 caacgtcggc acgattcccc ccctcacgga tctgtccgac gccgaccgcg cggcgctcgc
     2941 ccagtccgcg atcggattcg agacggtggg gcgcctcatc gggcggcagc gtcaacgcca
     3001 ggcgatcggc gaggccatgc gcgtcgttgg ggacgcgaac aagtacctcg ccgagatgga
     3061 gccgtggaag ctcaaggcga cggatccggc caggatggcc accgtcctcg gcgtcgccgc
     3121 gcaactcgtg tccgacgcca atacgctcct cgcgccgttc cttccgcact ccgcccagcg
     3181 cattcacgag gcactgggtg gccaggggac cttcgcgccg atgccgagga tggaggaggt
     3241 cgaggacctc gaccttccgg gccgcagtta cccgatcatc accggggact acacgggcgt
     3301 gcgcgacacc tgggccaggc acgacctcgt cgcgggcacc ccgattccgg cgcccgtgcc
     3361 cgtcttcacc aagctcgacg actcgatcgt cgacgacgaa ctcgcccgca tgcgcgggga
     3421 ggagtaggcg atcgctcgac cggggcggtg gcccccgagc cccgagccgc tgccccttcc
     3481 ggtggcggac aaccacgcgc acctcgagtg gcccgcgggc gacgagccca agaccgtcgc
     3541 cgagcgcttg gccgacgccg cggccgtcgg ggtcgatcgc gtcgttcaga tcgggtgcga
     3601 ggtggccagc gcacgctgga cggcgctggc cgtggtccag tacccgacgc tggtgggcgg
     3661 cgtggcgatt caccccaacg aggccccgct ccacgaggcc ggcacgcacg agagcggact
     3721 cggctacgac gaggcgttcg cgatcatcgc ggaactggcg cggggcccgc gcatgcgggt
     3781 gatcggcgag acgggcctcg atttctaccg cacgggccca gagggccgcg acgcgcaggt
     3841 cggcgcattc agggatcaca tcgcgctcgc caaggaactc ggcctcgtcc tccagatcca
     3901 cgaccgagac gcgcacgcag aggtggtcga ggtcctcgat cgcgacggcg cgcccgaccg
     3961 cacggtgatg cactgcttct ccggagacgc gggactcgcg agactctgcg tcgatcgcgg
     4021 ctggtacctc agcttcgccg ggacgctgac gttcaagaac tcgcacgaac tccgcggggc
     4081 cctcgccgtc acacccctgg accgcgtcct cgtcgagacg gacgccccct tcctcgcgcc
     4141 gcacccgcat cgcggcgccc ccaacgcccc ctacctcgtc ccgtggacga tgcgggagat
     4201 cgcgggcgtc cacgccgtgg cgcttgagga ggcgtgcgcc gcggtttcgg cgacctccga
     4261 cgc
//
LOCUS       DASVKP010000144         3964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_130607, whole genome shotgun sequence.
ACCESSION   DASVKP010000144 DASVKP010000000
VERSION     DASVKP010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130607"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1052
                     /gene="holA"
                     /locus_tag="VF362_02945"
     CDS             54..1052
                     /gene="holA"
                     /locus_tag="VF362_02945"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX7589916.1"
                     /translation="MEQRDGRRMGPQGWDRASSAPLVLVAGPEPVLAERAIVAIIADV
                     RSTDGTVSVTRLSAANYASGALRAATSPSLFAEPGVVVVDEGEAMNDAFADDALAYAR
                     APDPDVVVVLVHGGAVRGKKVLDAFRAAGAREFLCPAIKKDSDALAFVEGEFSRANRT
                     ARPQAIRALVDAIGADAPELAAACHQLMSDVKGPISPDEVTRYYGSRVNATGFAVADA
                     AIAGKVGEALALVRHALDTGTDPVPLIAAVAAKIRTLAKVGASRGRGLDPARDLGLAP
                     WQVERARRDLRLWTAETIAQAIEALAEADAQVKGAGRDPRFAVERAVRTIAGLAAN"
     gene            complement(1049..1747)
                     /locus_tag="VF362_02950"
     CDS             complement(1049..1747)
                     /locus_tag="VF362_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7589917.1"
                     /translation="MTELPVAQPRVWHLPQAGSGVESTVPRVLLGRQGIFSDARELVG
                     FELLFRAPGREGLRVDLWNARQQDRATEHVLAAAFFGGADVSEGLPLFVNFTRSYLVN
                     RESYLRAPHEIVLEVVESAHADASLFDRLAELKVAGYRVAIDDFLGTDSQRRLLEFAD
                     FVKIDYRDLASRGPRLVAAARAGGATLIAERIETEATFEECLDLGFEQFQGHLLEKAV
                     VLERAEFAIEGVGP"
     gene            complement(1814..2074)
                     /gene="rpsT"
                     /locus_tag="VF362_02955"
     CDS             complement(1814..2074)
                     /gene="rpsT"
                     /locus_tag="VF362_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX7589918.1"
                     /translation="MANIKSNIKRIGTNEKARIRNVAVKSELKTHVRRVREAIAAGDK
                     KTAESAFKLASVKLDKAASKGVIHENQAANRKSALAKQVAAL"
     gene            complement(2145..2711)
                     /locus_tag="VF362_02960"
     CDS             complement(2145..2711)
                     /locus_tag="VF362_02960"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX7589919.1"
                     /translation="MSDLTNALIAAAALAALTALRAATASKNRASKDRASKTTLRGRP
                     TTKPSRPGAAYPGDFAGSVPVSYTPKINRIPDPGEIVWAWVPYEEDFAQGKDRPVLLI
                     GRDGDWLLGLMLTSKDHNRDAADEARWGRHWMDIGAGSWDSHGRPSEIRLDRVVRLDP
                     RQVRREGSILDEPRFDAIISAARTVNHW"
     gene            complement(2708..>3964)
                     /locus_tag="VF362_02965"
     CDS             complement(2708..>3964)
                     /locus_tag="VF362_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7589920.1"
                     /translation="RLAHALAAHHRRDTTVAVLFFDVDHFNLVNDSLGHESGDALLAV
                     IGQRVQGAVRAGDTVARLGGDEFVVVLESISTQAAAEAVARTISSAVQCPISLAGHEV
                     VPTISVGLAIADGDVTAESLVRDADTAMYAAKQLGRARIEIFTPDLRETALNKLSIEA
                     ELRVAVRDGELAVHYQPVVELETRAVIAYEALVRWQHPTRGLLMPAEFIEISEEANLV
                     VPLGSFVLHEACGFLARHPEFTGRMFINVSTRQIGSADLSRAVRAALDSSGVDARRIS
                     LEITESGMLMTTKVAQADLEKIAEMGVDLVLDDFGTGYSALASIIQKPFAGLKPAQEF
                     TQHLGDNGSGDRISSAVASLAVSLGMYGVVEGVETEDQHACATRDGWPYGQGYLYGRP
                     MPENMVSFMDAGRARGTVGDRVGSEP"
BASE COUNT          593 a         1323 c         1408 g          640 t
ORIGIN      
        1 gtccgcggcc tgtgcgtccg cctcaggtac gtccgccgtg gcaggctggg ggcatggagc
       61 aacgggacgg acgacggatg ggtccgcagg gatgggaccg ggcctcctcg gcgcccctcg
      121 ttctggtggc tggccccgag ccggtgctcg cagagcgggc gatcgtcgcg atcatcgccg
      181 acgtccgatc gaccgacggg accgtctccg tgaccaggct ctcggccgcg aactacgcct
      241 caggggccct ccgtgccgcg acgagcccga gcctcttcgc cgaaccgggg gtggtggtgg
      301 tggacgaggg cgaggcgatg aatgacgcct tcgcggacga cgcgctcgcc tacgcccgcg
      361 cgcccgatcc cgacgtggtc gtcgtgctcg tccacggggg ggcggtacgc ggcaagaagg
      421 tgctcgacgc cttccgcgcc gcgggcgccc gcgagtttct gtgcccggcg atcaagaagg
      481 actccgacgc gctcgcgttc gtcgaggggg agttctcgcg ggcgaaccgg accgctcgcc
      541 cccaggccat tcgcgcgctc gtggacgcaa tcggggcgga cgcgcccgag ctcgcggccg
      601 cgtgtcacca actcatgagc gacgtcaagg gtcccatctc ccctgacgag gtcacgcgct
      661 actacggaag cagggtcaac gccacgggct tcgccgtggc cgacgccgcg atcgcgggga
      721 aggtgggcga ggcgctcgcg ctcgttcgcc atgccctaga cacgggcacc gatcccgttc
      781 ccctgatcgc tgcggtcgcg gccaagatcc ggacgctcgc gaaggtgggc gcatcgcgag
      841 ggaggggact cgatcccgcg agggatctcg gcctcgcccc gtggcaggtc gagcgcgcgc
      901 ggcgcgacct gcgcctgtgg acggccgaga cgatcgccca ggcgatcgag gcgctcgcgg
      961 aggccgatgc ccaggtcaag ggcgcgggtc gggatccgcg attcgcggtc gaacgcgcgg
     1021 tgcgcacgat cgcgggccta gctgcgaact agggccccac gccctcgatt gcgaattccg
     1081 cccgctcaag gacgacggcc ttctccaaga ggtgcccctg gaactgctcg aaccccaggt
     1141 cgaggcactc ctcgaaagtt gcctccgttt cgatccgctc ggcgatcagc gtcgcccctc
     1201 ccgctcgcgc cgcggcgacc aggcgcggtc cccgagacgc gaggtcgcga tagtcgatct
     1261 tcacgaagtc ggcgaactcg agtagccgcc tctggctgtc ggttccgagg aagtcatcga
     1321 tggcgacccg gtagccggcc accttcaact cggcgagccg gtcgaagagg gaggcgtcgg
     1381 cgtgcgcgga ctcgacgact tcgagaacga tttcgtgggg cgcacggagg tagctctcgc
     1441 ggttgacgag gtaggaccgc gtgaagttga cgaagagggg gagcccttct gacacatcag
     1501 cgccgccgaa gaacgccgcg gcgaggacgt gctcggtggc gcgatcctgt tgccttgcat
     1561 tccacaggtc gacgcggagg ccttcgcgac ccggagccct gaagaggagt tcgaagccaa
     1621 cgagttcgcg agcgtcggag aagattcctt gccgtcccag gaggacccgg ggaacggtcg
     1681 actccacacc ggagcccgcc tgcggaaggt gccacaccct gggctgggcc acggggagtt
     1741 cggtcacact ccagacccta gcgatgcatt gcgcggctgg atgacacatc cgtctcccag
     1801 cgcggcgccg ggcctagagc gcggcgacct gcttggccag cgccgacttg cggttggccg
     1861 cctggttctc gtggatgacg cccttgctgg ccgccttgtc cagcttgacc gacgccaact
     1921 tgaacgcgga ctccgcggtc ttcttgtcgc ccgcagctat cgcctcgcgg acgcggcgca
     1981 cgtgggtctt gagttccgac ttcacggcga cgttgcggat gcgggccttt tcgttggtgc
     2041 cgatccgctt gatgttcgac ttgatgtttg ccacgagggt ccttgcgctc tgggagggaa
     2101 ctgaaagatg tgcggccgtc ccggcgcaca tgcgaccacc catgctacca gtggttcacc
     2161 gtgcgggccg cgctgatgat ggcgtcgaat cggggctcgt cgaggataga tccctcgcgc
     2221 cggacctgcc gtggatcgag cctcaccacg cggtcgaggc ggatctcgct cgggcggcca
     2281 tgggaatccc acgatccggc gccgatgtcc atccagtgcc gcccccagcg cgcctcatcc
     2341 gcggcgtcgc ggttgtggtc cttgctggtg agcatgaggc cgaggagcca atcgccgtcg
     2401 cggccgatga gcaggacggg gcgatccttg ccctgcgcga agtcctcctc gtagggcacc
     2461 caggcccaca cgatctctcc cgggtccgga atccggttga tcttcggggt gtacgagacg
     2521 gggacgctcc ccgcgaagtc gccggggtac gcggcgcctg ggcgggaggg tttcgtcgtc
     2581 gggcgccccc gcagcgtcgt cttggacgcc ctgtccttgg acgctctgtt cttcgaggca
     2641 gtggcggccc ggagcgccgt gagcgccgcc agggcggcgg ccgcgataag ggcgttggtg
     2701 aggtcgctca tggttcgctg ccgacgcggt cgccgaccgt cccccgcgcc ctccccgcgt
     2761 ccatgaacga gaccatgttc tctggcatcg gacgaccgta caggtatccc tggccgtagg
     2821 gccacccgtc tcgcgtggcg cacgcatgtt gatcctccgt ctcgaccccc tcgacgacgc
     2881 cgtacatgcc gaggctgacc gcgagggagg caacggcgga actgatccgg tcgccggatc
     2941 cgttgtcgcc gaggtgctgg gtgaactcct gcgcgggctt gagcccggcg aagggcttct
     3001 ggatgatcga ggcgagcgcc gagtagccgg tcccgaagtc gtcgagaacg aggtcgacgc
     3061 ccatctcagc gatcttctcc aggtcggctt gcgcgacctt ggtggtcatg agcatccccg
     3121 actcggtgat ctccagggag atgcggcgag catcgacgcc cgatgagtcg agtgccgccc
     3181 tgacggcgcg cgagaggtcg gccgagccga tctgccgggt cgagacgttg atgaacatgc
     3241 gccccgtgaa ctcggggtgg cgcgccagga atccgcaggc ctcgtgaagc acgaaggagc
     3301 caagcgggac gacaaggttt gcctcctcgg agatctcgat gaactccgcg ggcatgagca
     3361 gccccctcgt ggggtgctgc catcgcacga gcgcctcgta cgcgatgacg gcgcgggtct
     3421 cgagttcgac gaccggctgg tagtggaccg cgagctcgcc atccctcacg gccacgcgaa
     3481 gttccgcctc gatcgacagc ttgttgagcg cggtctccct gaggtccggg gtgaagatct
     3541 cgattctcgc tcgtccgagt tgcttggccg cgtacatcgc cgtgtccgcg tcccggacga
     3601 gggactccgc cgtcacgtcc ccgtcggcga tggcgagacc gacgctgatg gtcgggacga
     3661 cctcgtggcc cgcgagagag atggggcatt gcacggcact gctgatggtc cgtgcgacgg
     3721 cttccgcggc ggcctgggtc gagatgcttt cgagcacgac gacgaactcg tcgcccccca
     3781 atcgcgcgac ggtgtctccc gcacggacgg cgccctggac gcgctgacct atgacggcaa
     3841 ggagagcgtc ccccgactca tgcccgaggg agtcgttgac gaggttgaag tggtcgacgt
     3901 cgaagaagag aacggcgacg gtggtgtcgc ggcgatggtg cgccgcgagg gcatgcgcga
     3961 ggcg
//
LOCUS       DASVKP010000145         6992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_628388, whole genome shotgun sequence.
ACCESSION   DASVKP010000145 DASVKP010000000
VERSION     DASVKP010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628388"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VF362_02970"
     CDS             <1..509
                     /locus_tag="VF362_02970"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX7589921.1"
                     /translation="WGRAVRDDVGLFERIALEGFQSGLSWATVLRKRENFRTAFADWD
                     APAIAGFGDDDVARLMDDVGIIRNRVKIDSAITNARALVALWDSGETLVDLVWAHAPA
                     APRPGPLHSWADIPTSTEESRALAKALKARGFVFVGPITMYALMQACGLVDVHLDACP
                     ARSRFGRP"
     gene            complement(584..1072)
                     /locus_tag="VF362_02975"
     CDS             complement(584..1072)
                     /locus_tag="VF362_02975"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEX7589922.1"
                     /translation="MTSRLVVAAALVDDLDLPRALLAARRSSPPDMAGLWEFPGGKVE
                     RGETPEDALRRELREELGVDVELGAELAGPEDGGIGEHGGAAGRVWPLGVVAASGHPL
                     AMRLWLARVTSGIPRPLEDHDEVRWLGPGQWRDVPWIPADLGIVDALLAEVVRRHRAT
                     WC"
     gene            complement(1110..1493)
                     /gene="gcvH"
                     /locus_tag="VF362_02980"
     CDS             complement(1110..1493)
                     /gene="gcvH"
                     /locus_tag="VF362_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX7589923.1"
                     /translation="MANIPDELMYSEEHEWVAGDISPGSEVAVGITEYAAAALGDIVY
                     VEAPEVGDDVVAGEVCGELESTKAVSEFFAPVSGVVTAVNDAVIDAPETLNTEAYGNG
                     WIFKIKVKTPNKALLSSEQYAAITE"
     gene            complement(1518..2705)
                     /gene="gcvT"
                     /locus_tag="VF362_02985"
     CDS             complement(1518..2705)
                     /gene="gcvT"
                     /locus_tag="VF362_02985"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX7589924.1"
                     /translation="MSAASVDALHASPLASRHAELGATMTDFAGWSMPVRYSSDLGEH
                     RAVRTAAGLFDVSHMGEIWLRGRGAGAALDVAVVSWISRVAVGRAKYTMICAADGGVI
                     DDLIVYRTDEETFLVVANAGNAAVVSAELSSRCAGDACEVEDRSDATSLIAVQGPASR
                     GIVDAMTGGLPRALKYYAATRATVDGEVAALVGRTGYTGEDGYEFFVATDDAGRLWDA
                     ALAAGAGDGLVPCGLAARDTLRLEAGMPLYGHELGLGYTPFAAGLGRVVQFGTSDDPR
                     GDFVGRDALESAERDAVLWDAEPRLAPPEARVLVGLTGEGKRAARAGYRILDGFGAEA
                     GEVTSGAPSPTLERPIAMAYVHPRFSALGTRVVADVRGRGEQMRVAALPFYQRPVAVT
                     PGA"
     gene            complement(2702..5647)
                     /gene="gcvP"
                     /locus_tag="VF362_02990"
     CDS             complement(2702..5647)
                     /gene="gcvP"
                     /locus_tag="VF362_02990"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019482074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEX7589925.1"
                     /translation="MVTLFSSRHIGPRARDARAMLEVLGRDSVGDLVEAAIPAEIFED
                     VPLDLPQALTEHEVLERLSRFAARNKAQVQMIGQGYSDTITPRVLRLNLWESPAWYTA
                     YTPYQAEISQGRLEALLVFQTMVADLTALPTAGASLLDEATAVAEAVLLMRRAVRGKE
                     GGVIVLDDECLPQTIAVVAAQAEALGIPLRVENLGPTWVAPKDLVGVVFQQPGVSGVL
                     RELGHIVESAHGVGALVTIAADILALCIITPPGEEGADVAVGSTQRFGIPLFFGGPHA
                     AYISVREGLERQLPGRLVGVSVDAEGRPAYRLALQTREQHIRRERATSNICTAQALLA
                     IAAGMYAVHHGPEGLRAIAARVHASAVSLADALRDAGVAVEHEAFFDTIMALVPGGAE
                     SVVARASAAGINLRRVGDDRVAIACDEITTPEIISRVVGAFVAREPRPIAASPSVAIP
                     RQLRRRSEYLFHSTFHRYRSETELMRYLRRLSDKDLALDRTMIPLGSCTMKLNAAVEM
                     APLTWPGFATIHPFAPSDQTAGYRDMISELERWLASITGYAAVSVQPNAGSRGEYAGL
                     LAIRSYHRANGQADRDVCLIPTSAHGTNAASAALAGMRVVPVETTSGGSIDVKDLKAK
                     LTAHKGAVACIMITYPSTFGVYEEGVPEVCALVHKAGGQVYIDGANLNALVGLAAPGH
                     FGGDVSHLNLHKTFAIPHGGGGPGVGPVAVAEHLVPYLPGLRQTIKRPEELARAGAPV
                     SGAPYGSAGILPVSWAYIALMGSDGLKRATEMAVLAANYVAARLDEHFPVLYKGPNGL
                     VAHECILDVRPVTERTGVTAEDIAKRLIDFGFHAPTMSFPIPGTLMVEPTESESREEL
                     DRFIEAMIVIREEIADIEEGRIAVEASPLRNAPHTASMVATDAWNQAYSRDQAAFPVV
                     SLRASKYWPPVRRIDNARGDRNLVTVLPLLDYVDTFRESRPGRDSEAEGAAEGEDPYR
                     ETPTDRAGE"
     gene            complement(5708..5893)
                     /locus_tag="VF362_02995"
     CDS             complement(5708..5893)
                     /locus_tag="VF362_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEX7589926.1"
                     /translation="MKFTIHKSAKNGQFWFRVVASNGQIMATSEMYTAKASVVNAIES
                     IQKSAGAAPIVDETAAK"
     gene            6023..6439
                     /locus_tag="VF362_03000"
     CDS             6023..6439
                     /locus_tag="VF362_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589927.1"
                     /translation="MQLDFAAIALGVSGRYGIGDDRCGAWPPDVLEPPPNERLTVDVL
                     LQSIIAGVAGGVGSSGTAATKPTPNKQGWLNTIAGVVGGIGGNFALGGTITDMLNGAG
                     GTTSTVQTGTGSGIGGLIGGIVLSLIVGWLQKRMTK"
     gene            6495..>6992
                     /locus_tag="VF362_03005"
     CDS             6495..>6992
                     /locus_tag="VF362_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019619601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7589928.1"
                     /translation="MKLVIQVPCLNEEATLALVLGSMPTKVPGIETIEIVVIDDGSTD
                     GTAEVARSHGVTHLVHHARTMGLARSFRDGVEYALGLGADIVVNTDGDNQYPQERIGD
                     LVQPILRGEAEIVIGDRQTSTIKHFSPFKRVMQRLGSWAVNRAAGTTLPDAASGFRAY
                     SRRSLY"
BASE COUNT         1100 a         2463 c         2365 g         1064 t
ORIGIN      
        1 aatggggacg cgcggtcagg gacgacgtgg gcttgttcga gcgcatcgcg cttgagggct
       61 tccagtcggg gctcagctgg gcgacggtgc ttcgcaagcg ggagaacttc cggacggcct
      121 tcgccgactg ggacgcgccg gcgatcgcgg ggtttgggga cgacgacgtt gcccggctca
      181 tggacgacgt cggcatcatc cgcaatcgcg tcaagatcga ctcggccatc accaatgccc
      241 gggcgctcgt ggcgctgtgg gattccggtg agacgctcgt ggatctggtg tgggcgcacg
      301 caccggccgc tccccgtcct gggcccctcc actcctgggc ggacatcccg acctccacgg
      361 aggagtcgag ggcgctcgcc aaggctctca aggcacgcgg cttcgtcttc gtcggaccca
      421 tcacgatgta cgccctcatg caggcctgcg gcctggtcga cgttcacctc gacgcgtgcc
      481 ccgcgcgatc gaggttcggt cggccctagc gtcgcccgcg ctcgtctgcc gaacttcgcc
      541 ccgtcccaac gtccggactc gttccgatcc cccggcgcgg gcctcagcac caggtcgcgc
      601 gatgcctcct gacgacctcg gcaagcaggg cgtcgacgat gccgaggtcc gcaggaatcc
      661 acggaacgtc gcgccactgg ccggggccga gccaccggac ctcgtcatgg tcctcgagcg
      721 gacgcggtat ccccgaggtc acccgcgcca gccagagccg catggcgagc ggatgacccg
      781 acgcggcgac caccccgagg ggccacaccc gcccggcggc tcctccgtgc tcgccgattc
      841 cgccgtcttc cggtcccgcg agttcggcac cgagttccac gtcgacgccg agctcctcgc
      901 gcagttcgcg gcgaagggcg tcctcgggcg tctccccccg ctccaccttc ccgccgggga
      961 actcccacag tcccgccatg tcgggagggg aggatcggcg cgcggcgaga agggcgcgcg
     1021 gtaggtcaag gtcgtcgacg agcgcggccg ccaccacgag gcggctcgtc acggcggtca
     1081 cggaacagcc acggaccggc cggggcgcgt cactccgtga tcgcggcgta ttgctcggag
     1141 ctcaggagcg ccttgttcgg cgtcttgacc ttgatcttga agatccagcc gttgccgtag
     1201 gcctcggtgt tgagcgtctc cggtgcgtcg atcacggcgt cgttcacggc ggtcaccacg
     1261 ccggagacgg gcgcgaagaa ctcggacacg gccttggtcg actcgagttc gccgcacacc
     1321 tcgcccgcga ccacgtcgtc ccccacctcg ggagcctcga cgtacacaat gtcgcccagc
     1381 gccgctgccg cgtactccgt gattcccacg gcgacctcgg agccggggga gatgtctcca
     1441 gcgacccact cgtgctcctc cgagtacatg agttcgtcgg ggatgttggc catgtcggtc
     1501 acctctctac gaaggtccta ggcgccgggc gttaccgcaa cgggccgctg atagaacggt
     1561 agcgcggcaa cgcgcatctg ctcacccctg ccgcgaacat ccgcgacaac tcgcgtgccc
     1621 aaggctgaga atcgcggatg gacgtaggcc atcgcgatgg gacgttccaa cgtgggcgat
     1681 ggggcgcccg acgtcacctc gcccgcctcc gcgccgaagc catcgaggat gcggtagccc
     1741 gccctggcgg cccgcttccc ctccccggta aggccaacga gcacccgcgc ctccggtggc
     1801 gcaaggcgcg gctccgcgtc ccacaggacc gcgtcccgct ccgcggactc cagcgcgtcg
     1861 cgccccacga agtccccacg agggtcatcc gacgtcccga actgaacgac tcgcccgagc
     1921 ccggcggcaa agggcgtgta cccgaggccg agctcgtggc cgtagagcgg catccccgcc
     1981 tcgaggcgga gcgtgtcgcg cgcggccaac ccgcacggaa cgagaccgtc gcccgccccg
     2041 gccgcgagag cggcgtccca caggcgcccg gcgtcgtccg tcgcgacgaa gaactcgtac
     2101 ccatcctcgc cggtgtagcc cgtccgcccc acgagtgccg ccacctcgcc gtcgaccgtc
     2161 gcccgtgtcg ccgcgtagta cttgagcgcg cgcggaaggc ctcccgtcat cgcgtcgacg
     2221 attcctcgcg acgcagggcc ctgaaccgcg atgagagagg tcgcgtcgga tcggtcttct
     2281 acctcgcatg cgtcgcccgc gcaccgggac gagagttcgg cggacaccac ggcggcgttg
     2341 cccgcgttcg cgaccaccag gaaggtctcc tcgtcggtgc gatagacgat gaggtcgtca
     2401 atgacgcccc cgtctgcggc acagatcatc gtgtacttgg cgcgaccgac ggcgactcgg
     2461 ctgatccaac tcacaaccgc gacgtccagg gccgcgcccg cacctcggcc ccgcagccag
     2521 atctccccca tgtggctcac gtcgaacagc ccggccgccg tgcgcacggc gcgatgctcg
     2581 ccgaggtccg aggagtagcg gacgggcatg gaccagccgg cgaagtcggt cattgtggcg
     2641 ccaagctccg catgccgact cgcgagtggg gacgcgtgaa gcgcgtcgac gctggcggcg
     2701 ctcactcccc ggccctatcg gtgggagtct cccggtaggg atcctcgccc tcggccgcgc
     2761 cctccgcctc ggagtcacgg ccgggccgcg actcgcggaa cgtgtccaca tagtcaagga
     2821 ggggaaggac ggtcaccagg ttcctgtctc cccgggcgtt gtcgatgcga cgcacgggtg
     2881 gccagtactt ggacgcgcgg agcgagacga cggggaaggc ggcctggtcg cgcgagtacg
     2941 cctggttcca cgcatccgtg gcgaccatcg atgcagtgtg cggcgcgttg cgcagcggcg
     3001 aggcctccac ggcgatgcga ccctcctcga tatccgcgat ctcctctcgg atcacgatca
     3061 tggcctcgat gaaccggtcg agctcctccc ggctctcgct ctccgttggt tcgaccatga
     3121 gggtgccggg aatggggaag gacatcgtgg gggcatggaa cccaaagtcg atgaggcgct
     3181 tcgcgatgtc ctccgccgtc acgcccgtcc tttcggtcac cggccggacg tcgagaatgc
     3241 actcgtgggc gacgagcccg ttcgggccct tgtagagaac cgggaagtgc tcgtccagcc
     3301 gagcggcaac atagttggcg gcgagcacgg ccatctccgt cgcgcgcttg aggccgtccg
     3361 agcccatcag ggcgatgtag gcccacgaca ccggaaggat cccggccgat ccgtagggtg
     3421 ccccgctcac gggcgcgcca gcgcgcgcaa gctcctcggg acgcttgatg gtctggcgca
     3481 atcccggcag gtacggcacc aggtgctccg ccacggcgac ggggccgacg ccggggccgc
     3541 ctcccccatg ggggatggcg aacgtcttgt ggaggttgag gtgggacaca tccccgccaa
     3601 agtggccggg ggcagcgagc ccaacgagcg cgttgaggtt cgcgccgtcg atgtacacct
     3661 ggccgcccgc cttgtgcacg agcgcacaca cctcgggcac gccctcctcg tagacgccga
     3721 acgtcgaggg ataggtgatc atgatgcacg cgacggcgcc cttgtgcgcc gtgagcttcg
     3781 ccttcaggtc cttgacgtcg atgctgccgc cggacgtcgt ctcgaccgga acgacacgca
     3841 tcccggcgag cgcagccgat gcggcgttcg ttccgtgcgc cgacgtcggg atgaggcaca
     3901 cgtcccgatc ggcctgacca ttcgcgcggt ggtacgagcg gatcgcgagc aatcccgcat
     3961 actcgccgcg ggagcccgca ttcggctgaa cggacacggc cgcgtacccc gtgatcgacg
     4021 cgagccaacg ctcgagttcg ctgatcatgt cgcggtatcc ggcggtctgg tcgctcggcg
     4081 cgaaggggtg gatcgtcgcg aatccgggcc acgtgagggg cgccatctcg accgcagcgt
     4141 tgagcttcat cgtgcacgat ccgaggggga tcatcgtccg gtcgagggcg aggtccttgt
     4201 cgctcagcct gcgcaggtac cgcatcaact ccgtctcgga gcggtagcgg tggaaggtgg
     4261 agtggaacag gtattccgac ctgcgacgaa gttggcgggg aattgcgacg ctcggactcg
     4321 cagcgatcgg tcgcggctcg cgcgccacga aggctccgac gacccgactg atgatctcgg
     4381 gcgtcgtgat ctcatcgcat gcgatcgcta cccggtcgtc tccgacgcgc ctcaggttga
     4441 ttcctgcggc cgacgcgcgg gcgacgacgg actcggcgcc gccgggcacg agcgccatga
     4501 tcgtgtcgaa gaaggcctcg tgttcgacgg cgacgcccgc gtcgcgcagc gcgtccgcga
     4561 gcgagaccgc gctcgcgtgc acccgagcgg cgattgctcg cagcccttcg gggccgtgat
     4621 gcaccgcgta catgcccgcg gcgatggcga ggagcgcctg cgccgtgcag atgttcgacg
     4681 tcgccctctc ccggcgaatg tgctgttctc tcgtttgaag cgcaagccgg taggccggtc
     4741 gtccttcggc gtcgaccgaa acccccacga gccgacccgg cagttgccgc tcgagcccct
     4801 cgcgtaccga gatataggcc gcgtgcgggc caccgaagaa gaggggaatc ccgaaccgct
     4861 gcgttgagcc gacggcgacg tcagccccct cctccccagg aggggtaatg atgcacagcg
     4921 ccaggatgtc cgccgcgatg gtgacgaggg cgcccacgcc gtgcgcggac tcgacgatgt
     4981 ggcccaactc gcgcaggacg ccagagacgc cgggctgctg gaatacgacg ccaacgaggt
     5041 ccttgggcgc gacccacgtg gggccgaggt tctcgaccct gagcggaatg cccaatgcct
     5101 ccgcctgcgc ggcgacgacg gcgatggtct ggggcaggca ctcgtcgtcg aggacgatga
     5161 cgccgccctc cttgccacgg accgcgcgcc gcatcagcag gaccgcctcg gcgaccgcgg
     5221 tggcctcgtc cagaagcgag gcgccagccg tgggaagagc ggtgaggtcc gccaccatgg
     5281 tctggaagac caagagggcc tcgaggcgcc cctgagagat ctccgcctgg tacggcgtgt
     5341 acgcggtgta ccaggccgga ctctcccaga ggttgaggcg caacacccgc ggagtgatcg
     5401 tgtccgagta cccctggccg atcatctgga cctgcgcctt gttgcgcgcc gcgaaacggc
     5461 tcaggcgttc gagcacctcg tgctcggtca gggcctgggg caggtcaagc gggacgtctt
     5521 cgaagatctc ggccgggatg gccgcctcga ccaagtcccc cacgctgtct cggccaagga
     5581 cttcgagcat cgcgcgggca tcgcgggctc gcgggccgat atgccgacta gagaacaagg
     5641 tcaccatccc atggtggcgc acggcgcgga cgcgcgaggc ggtcgaccca ccccgcgtgg
     5701 tccacggcta cttcgcggcg gtctcgtcga cgatgggggc cgcgcccgcc gacttctgga
     5761 tcgactcgat ggcgttcacc acagacgcct tcgccgtgta catctcactg gtcgccatga
     5821 tctgaccatt cgacgcgaca acgcggaacc agaactggcc gttcttcgcg ctcttgtgaa
     5881 ttgtgaactt cacgggacgt cctctcgttt gcaggtccac cgtcgagtct agggagtggg
     5941 cccctgcgaa gcgaggtccg ggcccgggtt tcttgcccga ccgcgctggc accggggcgc
     6001 gctgccgccc aaggcccctg gcatgcaact tgacttcgcg gccatcgccc tgggggtctc
     6061 cgggcgatac ggtatcggtg acgaccgctg cggggcgtgg ccaccagatg tgctcgaacc
     6121 gccgcccaac gagaggttga ccgtggacgt tctactgcag tcgatcatcg ccggagtcgc
     6181 tggcggggtg gggagttcgg gaaccgcggc gaccaagccg acgccgaaca agcaggggtg
     6241 gctcaacacc atcgcgggcg tcgtcggcgg catcggcggc aacttcgcgc tcggcggaac
     6301 catcacggac atgctcaacg gcgcgggagg cacgacctcg acggtccaga ccggcaccgg
     6361 gtccggcatc ggcggcctca taggcgggat cgtcctgtcg ctgatcgtcg ggtggctcca
     6421 gaagcggatg acgaagtagg cgccgcgcgc acgcccgata ggctggcacg acacaccacg
     6481 accgaagggt gccgatgaag ctcgtcattc aggtgccgtg cctcaacgag gaagccacgc
     6541 ttgcgctggt cctcggctcc atgccgacca aggtccccgg catcgagacg atagagatcg
     6601 tcgtcattga cgacggctcg acggacggca ccgccgaggt ggctcgaagc catggggtca
     6661 cccacctcgt gcaccacgcc aggacgatgg ggctcgcccg atcgttccgc gacggggtcg
     6721 agtacgcgct gggccttggc gccgacatcg tcgtcaacac cgatggcgac aaccagtacc
     6781 cgcaggaacg gatcggcgac ctcgttcagc ctatccttcg cggcgaggcc gagatcgtca
     6841 tcggcgaccg ccagacgagc accatcaagc acttctcacc cttcaagagg gtgatgcaac
     6901 gcctcggctc atgggccgtc aatcgggccg cagggaccac cctccccgac gcggcgtccg
     6961 gcttccgcgc ctattcgcgt cgatccctgt ac
//
LOCUS       DASVKP010000146         5444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1039541, whole genome shotgun sequence.
ACCESSION   DASVKP010000146 DASVKP010000000
VERSION     DASVKP010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039541"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VF362_03010"
     CDS             <1..381
                     /locus_tag="VF362_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771422.1"
                     /note="FabF; beta-ketoacyl-ACP synthase II, KASII;
                     catalyzes a condensation reaction in fatty acid
                     biosynthesis: addition of an acyl acceptor of two carbons
                     from malonyl-ACP; required for the elongation of
                     short-chain unsaturated acyl-ACP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEX7589929.1"
                     /translation="EAGGLSTQNVAHINAHATSTVVGDMIEARAIRTLLGAHADRTLL
                     SATKSMTGHLLGGAGAVESILTIKAIQDRKAPPTINVTQPDPELEVDLVCDIPRAMPS
                     GDVVGINNSFGFGGHNCALAFTNA"
     gene            complement(378..686)
                     /locus_tag="VF362_03015"
     CDS             complement(378..686)
                     /locus_tag="VF362_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589930.1"
                     /translation="MLADIQPGLATPIAGIRWLTEVQWAEPYTGPIPVLAETGALPLV
                     GAPQSEVFESAGFESAVLDVPQGPVGSIWDALDETAGIPILSRAALWRRDAVERRASA
                     "
     gene            complement(689..1186)
                     /locus_tag="VF362_03020"
     CDS             complement(689..1186)
                     /locus_tag="VF362_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HEX7589931.1"
                     /translation="MAAIACGVLFVHSATRAMCPHIEWAATNVVGSRASFDWTEQPAG
                     PNLLRCEVSWQGVQGTGARLASALRGWEHVRYEVTEEPSYGCDGGRWSHTPSLGIFHA
                     MTDVVGNIAVPEDRIRAAIDAGGNAADLKRAMDLALGSAWDEELEPFRYAGHGAPVRW
                     LHRVG"
     gene            complement(1328..1828)
                     /locus_tag="VF362_03025"
     CDS             complement(1328..1828)
                     /locus_tag="VF362_03025"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016664480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018206 - peptidyl-methionine modification
                     [Evidence IEA];
                     GO_process: GO:0031365 - N-terminal protein amino acid
                     modification [Evidence IEA];
                     GO_process: GO:0043686 - co-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX7589932.1"
                     /translation="MAMREIRVTGDPVLRTPCEPITDFSDAVRLLVADLLETVDTEGR
                     AGLSANQIGVGLRAFSWNIDGNVGYMLNPVVTVTSEEKQELAEEGCLSVPGLWFECDR
                     PAFARCEGTDLAGEPVAIEGEGIWARLILHETDHLDGKLFIDRLAKDVRKRALRQIRE
                     EIEHFG"
     gene            1862..2395
                     /locus_tag="VF362_03030"
     CDS             1862..2395
                     /locus_tag="VF362_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7589933.1"
                     /translation="MILETERLLLRPYRVSDAAAVHRYGSDPEVCRYTDFGPNTWDDT
                     LAFLQTAVHPIPPKIELAITLAGDDSVVGGVGAWPVAEGRWEMGWVLHRDVWGKGYAT
                     EAARAVFDAVAERGDVHTVFARCRPENPASARVMEKLGMRFVELIPRETEVRGEWVDS
                     QIYEAAIANAAIATAAP"
     gene            2392..2916
                     /locus_tag="VF362_03035"
     CDS             2392..2916
                     /locus_tag="VF362_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7589934.1"
                     /translation="MIVETERLLLRPYRLADATAIHRYGSDPEVSRYTEYGPNTWEDT
                     LRFLETAVRPIPPRIQLGITLRGDDAVVGGVGAWPVAEGRWEMGWGLRRDLWGHGYAT
                     EAMRAVFDLVAGRGDVHTIVARCRPENAASARVMEKLGMRLVEILPRDKQVGGEWMDT
                     EVYEVTLPGADVQA"
     gene            2921..4534
                     /locus_tag="VF362_03040"
     CDS             2921..4534
                     /locus_tag="VF362_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX7589935.1"
                     /translation="MSTPEVRWVATAAEIRAAEAEPLAREGEAALMDRAAAAVADAAA
                     GLLAERNIDAAGTRAVLLVGTGNNGGDALLAGALLAAAGATVTALQVGDTAHPRGLAA
                     LREAGGAVISRVAATGVGASSAAALLADANLVIDGIVGLGATAGLREPAASLVAAIPA
                     SVTVLAVDLPSGLDADSGLADSGATGSVRSRSADLGSVDSGSPAHVTAHATVSFTAPT
                     ACVLLPPAALAAGRVTFADVGVPIPPRKPGAPARLTDAGIAARWPVPEAGDNKYSRGV
                     VGIVAGSEAYPGAAILACTAAIRSGAGLVRFVGPRRAQDLVLAARPEVVAADPSLPLP
                     RVDAWVLGPGVADDPDQEAAILAAVASGVPCVVDAGGIMPVVLARALEGPGASGLLLT
                     PHAGELARALAGIGSTISADEIARDPAAAARTLAEATRATVLLKGAVTLVASPSGTLW
                     SQADGPAWLATAGAGDVLAGIAGALLAAGLPADEAGAVAASVHGRAARLVSGGGPIAA
                     LDIASALAAIVASLGSSIGDLLREGGRAT"
     gene            complement(4545..5333)
                     /locus_tag="VF362_03045"
     CDS             complement(4545..5333)
                     /locus_tag="VF362_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018778951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX7589936.1"
                     /translation="MVVARLRAAGCVFAEDEAWLIAEAATTHEECERLVRRRVAGEPL
                     EYVLGWAEFCGLRISVTPGVFVPRRRTEALVREAVRLLAPVAGEDNVVVDLCCGSGAI
                     AVAIRALAPSAEVYATDVDPAAVECARRNVEPTGGRVFQGDLFDALPAGLRGRVFVVV
                     ANAPYVPTEAIAFMPLEARDHEHRIALDGGSDGTELHRRIAAEAPAWLARGGHLLIES
                     SRAQGPTTARHMADAGLAVRVVEDDECDATVVIGDANSGARPKP"
BASE COUNT          766 a         1993 c         1915 g          770 t
ORIGIN      
        1 gaagccggag gcctctccac ccagaacgtc gcccacatca acgcgcacgc gacgtcaacg
       61 gtggtgggcg acatgatcga ggcgcgcgcc atccggacgc ttcttggcgc gcacgcggac
      121 cgcacgctcc tcagcgccac caagtccatg accggccacc tcctgggcgg cgccggggcg
      181 gtcgagtcga tcctcaccat caaggcgatt caggatcgca aggctccccc gacgatcaac
      241 gtcacgcagc ccgacccgga actcgaggtg gacctcgtgt gcgacatccc gcgcgccatg
      301 ccctcggggg acgtcgtggg gatcaacaac tcgttcggat tcggcgggca caactgcgcg
      361 ttggcgttca cgaacgctta ggcggaggcg cgccgctcca ccgcatctct gcgccacagc
      421 gcggccctgc tgaggatcgg aatccccgcc gtctcgtcga gcgcgtccca gatcgagcca
      481 acgggcccct ggggaacgtc gagcaccgcg gactcgaacc ccgcagactc gaacacctca
      541 gactgcggcg cgccgaccag cggcagggct cccgtctcgg cgagcacggg aatcgggccg
      601 gtgtagggct cggcccactg cacctcggtc agccagcgga tgcccgcgat cggcgtcgcg
      661 agccctggct ggatgtccgc gagcacgcct agccgaccct gtgcagccag cgaacgggcg
      721 cgccgtgtcc cgcgtagcgg aacggctcca actcctcgtc ccacgccgat ccgagcgcca
      781 ggtccatggc ccgcttgagg tccgcggcgt ttccgcccgc atcgattgcg gccctgatgc
      841 gatcctcggg gacggcgatg ttgcccacga cgtccgtcat ggcgtggaag atcccgaggc
      901 tcggcgtgtg gctccatcgg ccaccatcgc agccgtacga cggctcctcg gtcacctcgt
      961 agcgcacgtg ctcccagcct cgcaacgcgg aggccaggcg ggcgcccgtt ccctggacgc
     1021 cctgccacga gacctcgcag cggagcaggt tcggcccggc gggctgttcc gtccagtcaa
     1081 acgacgcgcg agatccgacg acgttggttg cggcccactc gatgtgtggg cacatggcac
     1141 gggtggcgga gtgtacgaaa agtacaccgc aggcaatggc agccatgacc cttccttcct
     1201 gtctaggtac gtcttcccca acgacctcaa caggaacggc agagccgtgc acggtgtgtg
     1261 cacttctatg gtgccctagg tgggcgaatt acgccagtgt gatacgcctt cggcgcgtcc
     1321 gagacgccta gccgaagtgc tcgatctcct cgcgaatctg tctgagcgcg cgcttgcgca
     1381 cgtccttggc gaggcgatcg atgaagagct tgccgtccag gtgatccgtc tcgtgaagga
     1441 tgagccgggc ccagataccc tcgccctcga tcgcgaccgg ctccccggcg aggtccgtgc
     1501 cctcgcagcg ggcgaacgcc gggcggtcgc actcgaacca cagcccggga acgctgaggc
     1561 agccctcctc cgcgagttcc tgcttctcct cgctcgtcac cgtcacgacg ggattgagca
     1621 tgtagccgac gttgccgtcg atgttccatg agaaggcgcg gaggcccacc ccgatctggt
     1681 tcgccgagag ccccgcgcgc ccctccgtgt cgacggtctc tagcaggtcg gcaacgagga
     1741 gccgcacggc gtcgctgaag tccgtgatgg gctcgcacgg cgtcctcaaa acggggtcgc
     1801 cggtcacgcg gatctctctc atcgccatgc gcgtattctt ccatgaccct acgatggacc
     1861 ggtgatcctc gagacagagc gactcctcct gaggccctat cgggtctcgg acgccgcggc
     1921 ggtccaccgc tacggctcgg atcccgaggt ctgccgctac acggacttcg ggcccaacac
     1981 gtgggacgac accctggcgt tcctccagac ggcggtccac cccatcccgc ccaagatcga
     2041 gctcgccatc acgctcgcgg gcgacgactc ggtggtcggg ggcgtggggg catggcccgt
     2101 cgcggagggc cgctgggaga tgggatgggt gctgcaccgc gacgtgtggg gaaagggata
     2161 cgcgacggag gcggcgcgcg cggtcttcga cgccgtcgcc gagcgcggcg acgtccacac
     2221 ggtcttcgcg cggtgccgtc cggagaaccc ggcgtcggcg cgggtcatgg agaagctggg
     2281 gatgcgcttc gtggaactca tcccacggga gacggaagtg cgcggagagt gggtggactc
     2341 gcagatctac gaggctgcca tcgcgaatgc cgccatcgcg actgccgccc cgtgatcgtc
     2401 gagaccgagc gactcctcct caggccctac cggctcgcgg acgcgacggc gatccatcgc
     2461 tacggctcgg accccgaggt atcccgctac acggagtacg ggccaaacac gtgggaggac
     2521 accctgcgct tcctcgagac ggccgttcgt ccgatcccgc ccaggatcca actgggcatc
     2581 accctcaggg gcgacgacgc cgtcgtcggc ggcgtcgggg cgtggccggt cgccgagggc
     2641 cgttgggaga tgggttgggg gctacgacgc gacctgtggg gacacgggta cgcgacggag
     2701 gcgatgcgcg ccgtcttcga cctcgtcgca gggcgcggcg acgttcacac gatcgtcgcg
     2761 cgatgccgcc cggagaacgc cgcctctgcg cgggttatgg agaaactggg gatgcgcctc
     2821 gtggaaatcc tcccccgcga caagcaggtt ggcggggaat ggatggacac cgaagtctac
     2881 gaggtgaccc ttccgggggc ggatgtgcaa gcctaggggg atgagcactc ccgaggttcg
     2941 ctgggtcgcc acggccgccg agatccgcgc cgcggaggcc gagccgctgg cccgcgaggg
     3001 cgaggcggcg ctgatggatc gcgcggcggc ggccgtggcg gacgcggccg caggcctgct
     3061 cgccgaacgc aacatcgacg ccgccggaac ccgggccgtc ctcctcgtcg gcacgggcaa
     3121 caacggcggc gacgcgctac tcgcgggcgc cctgctcgcc gcggcggggg cgaccgtcac
     3181 ggcgctccag gtcggcgaca ctgcgcaccc ccgagggctc gcggcgctgc gtgaggcggg
     3241 cggcgcggtg atttcccgcg tcgcggctac cggcgtcggc gcctcgtccg cggccgcgct
     3301 cctcgctgac gcgaacctcg tcatcgacgg gatcgtgggc ctcggcgcga ccgccggact
     3361 tcgggagccc gccgcctcac ttgtcgccgc gatccccgcg tccgtgaccg tgctcgccgt
     3421 tgaccttccg agcgggctgg acgccgactc gggcttggcc gactcgggcg ctacgggctc
     3481 ggtccgttca cgctcggccg acttgggctc ggtcgactcg ggctcgccgg cgcacgtgac
     3541 ggcccacgcg accgtctcct tcaccgcgcc gaccgcgtgc gtcctccttc ccccggcggc
     3601 cctcgccgcg ggccgggtca ccttcgccga cgtcggggtt ccgatcccgc cacgcaaacc
     3661 cggggcgccc gcgcggctga cggacgccgg gattgccgcg cgctggcccg ttcccgaggc
     3721 cggggacaac aagtactcgc gcggcgtcgt gggcatcgtc gcagggtcgg aggcctaccc
     3781 cggcgccgcg atcctcgcat gcacggccgc gatccgatcg ggagcggggc tcgtacgatt
     3841 cgtcggcccc cgtcgcgccc aggacctggt cctcgccgcc cgacccgagg tcgttgccgc
     3901 cgacccctcc ctgcccctgc cgcgcgtcga cgcgtgggta ctggggcccg gcgtggcgga
     3961 tgaccccgat caggaggcgg cgatcctggc cgccgttgcg agcggggttc cgtgcgtcgt
     4021 ggatgcaggg gggatcatgc cggtggtcct ggcgcgggcg ctggagggac ccggtgccag
     4081 cggcctcctc ctcacgcccc acgccggcga gctcgcgcgg gccctcgcgg gaatcgggag
     4141 caccatttcc gcggacgaga tcgcccgcga tcccgcggcc gccgctcgca ccctcgcgga
     4201 ggcgacgcgc gccacggtgc tcctcaaggg ggccgtgacg ctcgtggcga gcccctcggg
     4261 gacgctctgg agccaggcgg acggtcccgc gtggctagcg acggcgggcg caggagacgt
     4321 gctggcaggc atcgcagggg cgctccttgc ggctggcctc cccgccgacg aggcgggagc
     4381 cgtggcggcc tccgtgcacg ggcgcgcggc gaggctcgtc tctgggggcg ggccgatcgc
     4441 agcgctcgac atcgcctcgg ccctcgccgc catcgtcgct tccctcggct cctcgatcgg
     4501 cgacctcctg cgcgagggcg ggcgcgcgac gtagccgagc gcacctaggg cttcggacgc
     4561 gcgccggaat tcgcgtcgcc gatcaccacc gtggcgtcgc actcgtcgtc ttcgacgacg
     4621 cggacggcca acccggcgtc ggccatgtgg cgcgcggtcg tcgggccctg agccctgctc
     4681 gattcgatga gcaggtgccc gccgcgcgcg agccacgcgg gcgcctccgc cgcgattcgc
     4741 cgatggagtt cggtgccatc ggaaccgccg tcgagcgcga tccggtgctc gtggtcgcgg
     4801 gcctcgaggg gcatgaaggc gatcgcctcg gtgggaacgt agggagcgtt cgcgacgacg
     4861 acgaagacgc gcccccggag tccggcggga agcgcatcga agaggtctcc ctggaagacc
     4921 cgcccgcccg tcggctcgac gttccgccgg gcgcactcca ccgcggcagg gtcgacgtcg
     4981 gtggcgtaga cctccgcgga cggcgccagg gcccggatcg cgacggcgat cgcgcccgag
     5041 ccgcaacaga ggtcgacgac gacgttgtcc tcgcccgcga ccggcgcgag tagccgcacg
     5101 gcctcccgca cgagcgcctc cgtccggcga cgcggcacga acaccccggg ggtcacggaa
     5161 atccgaaggc cgcagaattc ggcccacccg aggacgtact ccaggggctc ccctgcgacg
     5221 cgacgtctca cgaggcgctc gcactcctca tgcgtcgtcg ccgcctcggc gatgagccac
     5281 gcctcgtcct cggcgaagac gcaccccgcg gcgcgcaacc gcgccacgac catgtcgccg
     5341 ggaccggcgc ttccgaccac gtcactcccc cgctcggcga gtcctgcgat tccggccatg
     5401 ctagtcgagc gcgcggtccg cgcggggcac ctacctgagg tcgg
//
LOCUS       DASVKP010000147         5543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1205942, whole genome shotgun sequence.
ACCESSION   DASVKP010000147 DASVKP010000000
VERSION     DASVKP010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205942"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /gene="ispG"
                     /locus_tag="VF362_03050"
                     /gene_synonym="gcpE"
     CDS             complement(<1..1095)
                     /gene="ispG"
                     /locus_tag="VF362_03050"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX7589937.1"
                     /translation="MPPLPPVPLAPRRVSRKIRVGSVAVGGDAPVSVQSMTTTLTANV
                     DATLQQIAELTAAGCDIVRVAVPSQDDADALKAIAKKSQLPVIADIHFQPRYVFDAID
                     AGCAAVRVNPGNIIKFDDKIAEIAKAAKDAGVSIRIGVNAGSLDPRLMEKYGRATPEA
                     LVESAMWEASLFEEHDFHDFKISVKHHDPVVMIQAYRLLALRGDWPLHLGVTEAGPTF
                     QGTIKSATAFGALLAEGIGDTIRVSLSAPPVEEVKVGIQILQSLGLKERKLEIVSCPS
                     CGRAQVDVYELAEKVSAGLEGMEVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIF
                     VRGEVVTTVPERDIVETLIFEAMKLAATMEP"
     gene            1306..1539
                     /locus_tag="VF362_03055"
     CDS             1306..1539
                     /locus_tag="VF362_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589938.1"
                     /translation="MTTQITVRLPDDAVAFIDAQVALGAAASRAALVGRAVERERRAV
                     VAERDAAIYATSTDDADLAAFTATAASATPDLG"
     gene            1545..1847
                     /locus_tag="VF362_03060"
     CDS             1545..1847
                     /locus_tag="VF362_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017825004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX7589939.1"
                     /translation="MRPIHTARLDKVRPVVLLTREAVVPYLARVTVAPITSTARGLST
                     EVPVGARNGLADASVISLDNIATIDRSALGRVIGYLTPAQELELARGIVTAFDLRE"
     gene            complement(1899..3221)
                     /locus_tag="VF362_03065"
     CDS             complement(1899..3221)
                     /locus_tag="VF362_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7589940.1"
                     /translation="MNHALLTTAGVLILALGVMLSIGLHEIGHMVPAKLFGVRVSQYM
                     VGFGPTLWSRTRGETEYGIKAIPAGGYVRLTGMIPPADEVRPVKGTGWAARAIEDARL
                     GSVEEILPGQEHRAFYRLSWGRKVIVMAGGPVVNLVIALVIFTASASIHGVFVAQPVV
                     GTVVDCVPAVDAAGVCAAGDPPSAASQAGLRAGDVIVSSDGVAVKDWASLSADIRAHG
                     GVPMTLGIDRGGQSLTLTVTPTAADVAAYGSNGLVLHNADGSIATKHVGFLGMSPTQV
                     IVRQNLAYGTTEMFSYLGSTAGMMVRLPALVYHAGRTALGLEARDPGGLVSVVGVGRI
                     GGEIASSDVTGYTFGDRVADMFGLVAALNLALFAFNMIPLLPLDGGHIASALWQGVKN
                     GWARVRALPMPRPVDVARMMPIAYAVFVLLMGMGLLLMYVDFVDPVKI"
     gene            complement(3231..4385)
                     /gene="dxr"
                     /locus_tag="VF362_03070"
     CDS             complement(3231..4385)
                     /gene="dxr"
                     /locus_tag="VF362_03070"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX7589941.1"
                     /translation="MTVRTVAVLGSTGSIGTQACDVIRRNRDRFDVVALAAGGANPEA
                     VIAQAREFGVSFVAVAAAGAADAVAAALPGVTVKAGERAIAEAAAIGADVVLNGMTGS
                     VGLGPTLAALGAGSTLALANKESLVVGGALVLAAKKRPDQIVPVDSEHSALAQALGAG
                     RREDVRRLVLTASGGPFRGRSRSDLAGVTPAEALRHPTWDMGPVVTINSATLVNKALE
                     VIEAHLLFGVPMDRIDVVVHPQSQIHSMVEFIDGSTVAQASPPDMRLPIALGLTWPER
                     LADVAPAYDWSEPRAWTFEPLDEDAFPAIRLARHAVAASALHPAVFNAANEVAVAAFL
                     AGTLPFLGIVDTIAATLDAFAAPGEMTLDAVLEAERWARQAAGHHIANWT"
     gene            complement(4388..4945)
                     /locus_tag="VF362_03075"
     CDS             complement(4388..4945)
                     /locus_tag="VF362_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX7589942.1"
                     /translation="MSKLFPRAGLLRSGYSVKQVDAFFDRVRQIYEQPLVDPGGLSPL
                     TIRQATFDLRYRGYRTDEVDTALDRLEDAFAKRLRDQFVQANGNAAWQQDLAARAQAL
                     YDNLRRDPGQRFSRPDGMGKGYDTKQVDLLLDRLTSFFDTGAPIAPEDIRTAAFARRL
                     KPRAYDEDEVDAYLAQAVDILQGVS"
     gene            complement(4942..>5543)
                     /gene="rlmN"
                     /locus_tag="VF362_03080"
     CDS             complement(4942..>5543)
                     /gene="rlmN"
                     /locus_tag="VF362_03080"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX7589943.1"
                     /translation="RLSNVVFMGMGEPLANYNAVLTAVRTLIAPEPDGLGLAARHVTV
                     STVGLVPAIDRLAGEGLQVTLALSLHAPDDELRSELVPINTQWSVDEALDAAKRYFDA
                     TGRRVSIEYALIRDVNDQAWRADLLGQKLAKRGRAWVHVNPIPLNPTPGSKWTASEPA
                     VEREFVRRLTEHAIPATIRDTRGSDIDGACGQLAIKESL"
BASE COUNT          855 a         1924 c         1952 g          812 t
ORIGIN      
        1 gggctccatc gtcgcggcga gtttcatcgc ctcgaagatc agggtctcga cgatgtcgcg
       61 ctctggcacg gtcgtgacga cctcgccgcg gacgaagatc tggcccttgc cgttgcccga
      121 cgcgacgccg aggtcggcct cgcgcgcctc gcccgggccg ttgacgacgc accccatgac
      181 ggcgacgcgg agcggcacct ccatgccctc gagcccggcg ctcaccttct ccgcgagttc
      241 atagacgtcc acctgggcgc gcccgcaact ggggcacgag acgatctcga gtttgcgctc
      301 cttgaggccg agggactgga ggatctggat gccgaccttg acctcctcca ccggcggcgc
      361 ggagagggag acacgaatgg tgtcgccgat gccctcggca agcagggcgc cgaacgccgt
      421 cgcggacttg atggtcccct ggaacgtcgg ccccgcctcg gtgacgccga ggtggagcgg
      481 ccagtcgccg cgcagcgcga gcaggcggta cgcctggatc atgaccacgg ggtcgtggtg
      541 cttgacggaa atcttgaagt cgtggaagtc gtgctcctcg aagagcgacg cctcccacat
      601 ggcggactcg acgagcgcct ccggcgtggc cctgccgtac ttctccatca ggcgcgggtc
      661 gagcgagccc gcgttgacgc cgatgcggat ggagaccccg gcgtccttgg cggccttggc
      721 gatctcggcg atcttgtcgt cgaacttgat gatgttgccc gggttgacgc ggacggcggc
      781 gcagccggca tcgatcgcgt cgaagacgta gcgcggctgg aagtggatgt ccgcgatcac
      841 cgggagctgg gacttcttcg cgatggcctt gagggcgtcg gcgtcgtcct ggctcgggac
      901 cgcgacgcgc acgatgtcgc atcccgccgc ggtgagctcc gcgatctgct gcagcgtcgc
      961 gtcgacgttc gccgtgaggg tggtcgtcat ggactggacg ctcacggggg cgtcgccacc
     1021 caccgccacc gagccgacgc gaatcttgcg cgagacgcgt cgtggggcga gagggacggg
     1081 gggcagcggg ggcatgccga gggatacgga agtcactccc ccagtatcgc gcacgttccc
     1141 tgaaccaggg tgcggggctc gaggcccggg tatggcgggc ctgtggaaac ggctccaccg
     1201 tcgcgcatcg cgggtacggt ggccgacgtg aaccgcgcac ccgctacccg ccccgcccgt
     1261 cccgaacgac ttgcccggtg ggattcacgg ccataccatt atgggatgac gacccagatc
     1321 accgtgcgac tcccggacga cgccgtggct ttcatcgacg cgcaggtcgc gctaggcgcg
     1381 gcggcgagcc gggcggctct ggtggggcgc gcggtcgagc gcgagcgccg ggccgtggtc
     1441 gccgagcgcg acgcggccat ctacgccaca tccaccgacg acgccgacct cgcggccttc
     1501 accgcgacgg ccgcatccgc gaccccggac ctcggctagc ccccgtgcgt ccgatccaca
     1561 ccgcgcgact ggacaaggtc cgccccgtcg tcctcctgac ccgagaggcg gtcgtcccgt
     1621 atctcgcccg cgtgacggtc gcgccgatca cctcgaccgc acgcggcctc tccaccgagg
     1681 tccccgttgg cgcgaggaat ggcctcgcgg atgcttccgt gatctccctc gacaacatcg
     1741 cgaccatcga tcgctcggca ctcggccgcg tgatcggcta cctcacgccg gcccaagaac
     1801 tggaactcgc gcgggggatc gtcacggcct tcgacctgcg cgagtgagag gaagcgtctc
     1861 cggcccgttg gggtcgcctc acgtccggtg ggacgccgct agatcttcac ggggtcgacg
     1921 aagtcgacgt acatcagcaa cagccccatg cccatgagca ggacgaacac cgcgtaggcg
     1981 atcggcatca tgcgggcgac gtccacgggg cggggcatgg gcaaggctcg gaccctcgcc
     2041 cagccattct tgactccctg ccatagggcg ctcgcgatgt gccccccgtc gagcggaagg
     2101 agcgggatca tgttgaaggc gaagagcgcg agattgaggg cggcgaccag cccgaacatg
     2161 tccgcgacgc ggtccccgaa cgtgtagccc gtgacgtccg acgaggcgat ctcgcccccg
     2221 atcctcccga cgccgacgac gctcacgagg ccgccggggt cgcgcgcctc gagcccgaga
     2281 gcggttcggc ccgcgtgata gaccaacgcg ggaaggcgca ccatcatgcc cgccgtggag
     2341 ccgaggtagg agaacatctc ggtcgtgccg taggcgagat tctggcggac gatgacctgg
     2401 gtgggcgaca tcccgaggaa cccgacgtgc ttggtcgcga tcgagccgtc cgcgttgtgc
     2461 aggacgaggc cgtttgagcc gtacgcggcg acgtcggccg ccgtcggggt gacggtgagg
     2521 gtcaaggact ggccgccgcg gtcgatgccg agcgtcatcg gcactccccc gtgcgctcgg
     2581 atgtccgcgc tgaggctcgc ccagtccttc accgcgacgc catcggacga gacgatgacg
     2641 tccccggcac ggaggccggc ctgggaggcc gcgctcggcg gatcgcccgc ggcgcacacg
     2701 ccggccgcgt caacggccgg aacgcagtcg acgacggtcc cgacgacggg ctgggcgacg
     2761 aagacgccat gaatcgacgc acttgccgtg aagatcacga gggcgatgac gaggttcacg
     2821 accggtccgc ccgccatgac gatgaccttc ctcccccacg agaggcggta gaaggcgcgg
     2881 tgctcctggc cgggaaggat ctcctcgacg gaacccagcc gcgcatcctc gatcgcccgc
     2941 gcggcccagc cggtgccttt caccgggcga acctcgtccg ccggcggaat catcccggtg
     3001 agccgcacgt acccgcccgc gggaatcgcc ttgatcccgt actccgtctc gccgcgcgtg
     3061 cgggaccaca gcgtcggccc gaagccaacc atgtattggc tcacacggac gccgaagagc
     3121 ttggccggga ccatgtggcc gatctcgtgg agcccaatcg acagcatgac cccgagcgcg
     3181 aggatgagga cgccggcggt ggtgagaagg gcgtgattca cgatgccacc ttacgtccag
     3241 ttcgcgatgt ggtgcccggc cgcctggcgg gcccaacgct cggcctcaag gaccgcgtcg
     3301 agcgtcatct cgccgggcgc ggcgaacgcg tcgagcgtag ccgctatggt gtcaacgatt
     3361 ccgaggaacg gaagtgttcc cgcgaggaac gccgccacgg cgacctcatt cgcggcgttg
     3421 aagaccgctg ggtgaagcgc cgaggccgcg acggcgtggc gggcgagccg gatcgcgggg
     3481 aaggcgtcct cgtcgagcgg ttcgaacgtc cacgcgcgcg gctcggacca gtcgtaggcg
     3541 ggcgcgacgt cggcgaggcg ctccggccag gtcaggccga gcgcgatggg aaggcgcatg
     3601 tccggggggc tcgcctgagc cacggtgctc ccgtcgatga actcgaccat cgagtggatc
     3661 tgcgactggg ggtggaccac cacgtcgatc cggtccatcg gaacgccgaa caggaggtgc
     3721 gcctcgatca cctcgagcgc cttgttgacg agcgtcgccg agttgatcgt caccacaggc
     3781 cccatgtccc acgtggggtg gcggagcgcc tccgctggcg tgacccccgc gaggtccgac
     3841 ctcgacctgc cccggaaggg cccccccgac gccgtgagca cgagccggcg gacgtcctcc
     3901 cggcgccccg cgcccagcgc ctgcgcgagc gcgctgtgct ccgagtcgac gggcacgatc
     3961 tggtccggcc gcttcttcgc cgcgaggacg agcgcgccgc cgacgacgag cgactccttg
     4021 ttggcgaggg cgagggtcga accagctccg agcgcggcga gcgttgggcc gagccccacg
     4081 gatcccgtca tgccgttgag cacaacgtcg gcgccgatgg ccgccgcctc ggcgatggcg
     4141 cgctcccccg ccttgaccgt gaccccgggc agtgcggccg cgacggcgtc cgcggcgccc
     4201 gcggccgcta ccgccacgaa agagacgccg aactcgcgcg cctgcgcgat gaccgcctcg
     4261 gggttcgctc cccccgccgc gagcgcgacg acgtcgaacc ggtcgcggtt gcgtcgaatg
     4321 acatcacacg cctgggtccc gatcgacccg gtcgagccca gcaccgcgac ggtgcggacg
     4381 gtcatgccta ggagacgccc tggaggatgt ccaccgcctg ggcgagatag gcgtccacct
     4441 cgtcctcgtc gtacgcgcgc ggcttgagcc tgcgggcgaa cgcagcggtg cgaatatctt
     4501 cgggcgcgat cggcgcgccg gtgtcgaaga atgacgtcag acggtcgagc aggagatcca
     4561 cctgcttggt gtcatacccc ttgcccatgc cgtccggccg ggagaatcgc tgcccaggat
     4621 cgcgacgcag gttgtcatac agcgcctgcg cacgggccgc gaggtcctgc tgccatgcag
     4681 cgttgccatt cgcctggaca aactggtcgc ggagccgctt ggcgaacgcg tcctccaggc
     4741 ggtcaagagc ggtgtcgacc tcgtccgtgc ggtacccgcg gtaccgcagg tcgaaggtgg
     4801 cctggcgaat ggtcagcggc gacaggccgc ccggatcgac gaggggctgc tcataaatct
     4861 gccgcacccg gtcgaagaac gcgtcgacct gcttgacgct gtagcccgag cgcaacaggc
     4921 ccgcgcgggg gaacagcttg ctcatagtga ctccttgatc gcgagttggc cacaggcccc
     4981 gtcaatgtcg ctaccccgag tatcccggat tgttgccgga atcgcatgtt ccgtgagccg
     5041 tcgaacgaat tcgcgctcca ccgcgggctc gctcgcggtc cacttgctac cgggcgtcgg
     5101 gttgaggggg atggggttga cgtgcaccca tgcgcgtccg cgcttcgcca gtttctgccc
     5161 gaggaggtcc gcccgccacg cctggtcgtt gacgtcgcgg atgagggcgt actcgatgct
     5221 gacgcggcgg cccgtcgcgt cgaagtagcg cttcgccgcg tcgagcgcct cgtcgacgga
     5281 ccactgggtg ttgatcggca cgagctcgct cctgagctcg tcgtcgggag cgtggagcga
     5341 aagcgcgagc gtcacctgga gaccctcgcc agcgaggcga tcgatggcgg ggacgaggcc
     5401 gaccgtggac accgtcacgt ggcgcgcggc gaggccgagg ccatccggct cgggagcgat
     5461 gagcgtgcgc accgccgtca ggacggcgtt gtagttggcg aggggttccc ccatccccat
     5521 gaagacgacg ttcgacaggc gcc
//
LOCUS       DASVKP010000148         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049 k141_49299,
            whole genome shotgun sequence.
ACCESSION   DASVKP010000148 DASVKP010000000
VERSION     DASVKP010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49299"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..505
                     /locus_tag="VF362_03085"
     CDS             332..505
                     /locus_tag="VF362_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589944.1"
                     /translation="MCSPATCRACGKPSYTGCGQHVERVLGHVPVHQRCKCTAEQKQA
                     ALPPSLLATLFRR"
     gene            complement(825..1388)
                     /locus_tag="VF362_03090"
     CDS             complement(825..1388)
                     /locus_tag="VF362_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589945.1"
                     /translation="MIQRIVLGILGGALIVLSVFTVGNSVAFDKGVPDSGAAWVVLSA
                     GALVALFAIVGVRTLIGFGAGAAAAVAATDIIDAVRAGDFTVTARLVVLVVGVLAALA
                     ATTGRRSKRVVVEPAPAEVAPVDPNPVAGKSVTTVVPAQPAKATVTTVSAEHATTSEK
                     TIPKSARKVRATPQVHRPDGDAVGTSE"
BASE COUNT          323 a          726 c          682 g          323 t
ORIGIN      
        1 cctacacgac gccggacgat cgggctggta cttctggatg gcccttatcc cgttcgccgg
       61 ggtgttcatc gttctctact acctggtcca agagggcacg aacggcgcca acacctacgg
      121 ccccgacccg aaggcgtagc tctagcccct ccccctcggt cacccggatc gactcgcgcc
      181 ccgtccccct cgtgggggcg gggcgcgagt cgtgcaagcg ccgccggttg cctcggtgcc
      241 cgctatggcg cgtccgcttg cgtcataccc cctggggtat gctaaggtca tcccgaaagc
      301 gtcacaaaga ccctcgagcg gagatcttca gatgtgtagc cccgccacct gccgcgcctg
      361 cggaaagccc tcgtacaccg gatgcggcca gcacgtcgag cgcgtgcttg ggcacgtccc
      421 cgtgcaccag cgctgcaagt gcacggccga gcagaagcag gccgcgcttc cgccgtcgct
      481 cctcgcgacg ctcttccgcc gctaggcaac gcgttcggcg tcgtcccggg acaccggggc
      541 gcgcggatgc ggcagggcgg ctaggaggcg ctcagcgcct ggcgccgtcg cgctcggctg
      601 tgacgacgac ggatgtggtc gccacggcga ccaggccaac cgtcgcgagg gcggcgagca
      661 gggcgatgat ggttgccttc atggcggtcc tttcggtggt acaagtacaa cgcccgaagg
      721 agccgattcc atgcaagcca aggctcatgt gcgcgaatcg ttatgttggc gatacgaatc
      781 cgtaatctac cggggcgtac gcgccgcagc tatccctctc tcccctattc cgatgtcccg
      841 acggcgtccc cgtccgggcg gtgaacttgg ggggtcgcgc gcaccttccg agcgctcttg
      901 gggatggtct tctccgaagt cgtcgcgtgc tccgctgaca cggtggtcac cgtggcctta
      961 gccggttgcg ccgggaccac cgtcgtgacc gatttgccgg cgaccgggtt ggggtccacg
     1021 ggcgcgacct ccgcgggcgc gggctcgacg accactctct tgcttcggcg accggtcgtc
     1081 gcagcgagcg cggcgaggac gcccacgacg agcacgacta ggcgcgcagt caccgtgaag
     1141 tccccggcgc gaacggcgtc gatgatgtcc gtcgcggcga cggccgcggc cgcgccagcg
     1201 ccaaagccga tgagggtacg cacgcccacg atcgcgaaga gtgccacgag tgcacccgca
     1261 ctgagcacga cccaggctgc gccgctgtcg gggacaccct tgtcgaacgc gaccgagtta
     1321 cccaccgtga agacggacag gacgatgagc gccccgccga ggattcccag cactattcgt
     1381 tgaatcatga cgtgctccct ttgtcgaggc gatcatttga ttggtggcga tcccttggtc
     1441 acgaggctac ccgcggtgcc catgtcaccg aggctaccct tcacctggtc tcgcctccaa
     1501 gactgaagcc aagacccgag cccggccgca gggccgtctt gtgggtcgcg agcggcgact
     1561 ggcggcgcgt tcccgcattt cgcgcgtggg gccccgtccg atgcccggaa gacatcgggc
     1621 gcgcgacgcg ttagaacttg tgacggaggg aggcggagtg ggctcacccc gacgcctgtg
     1681 cgcggcacca gtggccgacg cgggcgagca gccctacgac gcgcgggagg cgaccgaagc
     1741 cacccccctg gcaacggatc gtcgcgcggc gcccgcctcc ctcccgtcca cggcctcccg
     1801 cacgtgaggc gccccacgac gtgacacaca acactgcgct caccgagcga aattcccccg
     1861 gcgggtttag ccttgtggga gcgcgatgcc acaagatcgc gcggcctctg ggtagaagtg
     1921 gatggggcca catcagatag cacgccgtcg ttgtgcggag accctagccc ctccgcgccg
     1981 acgggcgaag tgtccctcgc aatcgcgggg tgacaggcaa ggccccggtc tgaccctcag
     2041 ccgaccgggg cttt
//
LOCUS       DASVKP010000149         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_296797, whole genome shotgun sequence.
ACCESSION   DASVKP010000149 DASVKP010000000
VERSION     DASVKP010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296797"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VF362_03095"
     CDS             complement(<1..394)
                     /locus_tag="VF362_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7589946.1"
                     /translation="MGLNPGALPDFPWDSLEPQRARASSHPDGIVDLSVGTPVDPTPE
                     LIRAALSSASNAPGYPTTQGRLDLREAIAAWFERRRGVPGLDPGAIFPTIGSKEFVAL
                     LPALLGLGAGDTVVIPEIAYPTYDVGARL"
     gene            complement(428..751)
                     /gene="fdxA"
                     /locus_tag="VF362_03100"
     CDS             complement(428..751)
                     /gene="fdxA"
                     /locus_tag="VF362_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX7589947.1"
                     /translation="MTYVIALPCVDVKDKACIDECPVDCIYEGERMLYIHPDECVDCG
                     ACEPVCPVEAIFYEDDVPDKWSEYYAANVEFFSVIGSPGGAAKMGLIKHDHDIIKALP
                     PQNAG"
     gene            complement(821..1186)
                     /locus_tag="VF362_03105"
     CDS             complement(821..1186)
                     /locus_tag="VF362_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589948.1"
                     /translation="MIRSTLAFAGLTFVGFLTGVVGAAEHRYHPYWGSILVIAVVLAS
                     ATFARASRSWFGLVVYASAWFLTAFLLFALHGPGDSVVILDDALGRTLFVGGALAIVA
                     VAFLPSAWMRDPSEWSPAE"
     gene            complement(1248..>2172)
                     /gene="ychF"
                     /locus_tag="VF362_03110"
     CDS             complement(1248..>2172)
                     /gene="ychF"
                     /locus_tag="VF362_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX7589949.1"
                     /translation="SELFHSERIMPATVSFVDIAGIVKGASEGEGLGNAFLSHIREAD
                     AICQVTRAFADPDVVHVEGRVDAASDLETINTELILADLQTLDKAIPRIEKEVRGKKA
                     SPDYLAACETARTILDGGTTLSAGAKAAGLELAAIRELTLLTAKPVIYVFNTDDAGLV
                     DDAQRAALAASVAPAEAIFLDAKFEAELVDLTPEESAEMLAESGQSESGLDQLARVGF
                     STLGLQTFLTAGPKESRAWTIRRGWTAPQAAGVIHTDFERGFIKAEVVGYRDLIAAGS
                     MAEAKAHGKVRIEGKDYVMADGDVVEFRFNV"
BASE COUNT          374 a          718 c          742 g          338 t
ORIGIN      
        1 cgaggcgcgc gccgacgtcg tacgtcgggt aggcgatctc ggggatgacg accgtgtccc
       61 ccgcgccgag gccgagaagc gcggggagga gcgcgacgaa ctccttcgac ccgatggtgg
      121 ggaagatcgc gcccggatca aggccgggaa cgccgcgccg acgctcgaac cacgcggcga
      181 tcgcttcgcg caggtcgagg cgtccctggg tcgtggggta ccccggcgcg tttgacgcgg
      241 aggagagtgc cgcccggatc agttcgggcg tgggatcgac gggggtcccg acagagagat
      301 caacgatccc gtccggatgc gacgacgccc tcgcgcgctg cggctcgagg gagtcccatg
      361 ggaagtccgg aagcgcgccc ggattcaagc ccacattcgc ctcaggctgc cgccggtcac
      421 cgtggctcta cccggcgttc tgcggaggga gcgccttgat gatgtcgtga tcgtgcttga
      481 tcagccccat cttcgccgcg ccccccggcg atccgatcac ggagaagaac tcgacgttcg
      541 ccgcgtagta ctccgaccac ttgtccggga cgtcgtcctc gtagaagatc gcctccacgg
      601 gacagacggg ctcgcaggct ccgcaatcga cgcactcgtc gggatggatg tagagcatcc
      661 gttcgccctc gtagatgcag tcgacggggc attcgtcgat gcacgccttg tccttgacgt
      721 cgacgcatgg cagggcgatg acgtaggtca cggcaactcc ttcggcgatc gttcgatcgg
      781 ggcttcatca tatcgccagg gatgcctgct ccccggcggc ctactccgct ggactccact
      841 cgctaggatc gcgcatccag gcggagggca ggaaggctac ggcgacgatc gcgagcgcgc
      901 cacccacgaa gagggtgcgg cctagcgcat cgtcgaggat gacgaccgag tcgcctgggc
      961 cgtggagcgc gaagaggaga aaggcggtga ggaaccacgc ggacgcgtac accaccagcc
     1021 cgaaccatga gcgtgacgcc cgcgcgaacg tcgccgaggc caggacgacg gcgatgacga
     1081 ggatcgatcc ccagtacggg tggtagcggt gttcggccgc gccgacgacc ccggtgagga
     1141 agccaacgaa agtgaggccg gcgaacgcga gggtcgaacg aatcatgcgg tccaccctac
     1201 gtcgcgggca agacttcctg gatcacaccg cggtacgggt gaggtgacta cacgttgaag
     1261 cggaactcca ccacgtcgcc gtcggccatg acgtagtcct tgccctcgat gcggaccttg
     1321 ccgtgggcct tggcttcggc catcgagcca gcggcgatga ggtcccggta gcccacgacc
     1381 tcggccttga tgaagcctct ctcaaagtcc gtgtgaatga cgccagcggc ctgaggcgca
     1441 gtccatccgc ggcggatcgt ccacgcgcgc gactccttgg gtcccgcggt aaggaacgtc
     1501 tggaggccca gggtcgagaa gccgacgcga gcgagttggt caagccccga ctcactctgc
     1561 ccggactccg cgagcatctc cgcggactcc tccggcgtga ggtccacaag ttccgcctcg
     1621 aacttcgcgt ccaggaagat cgcctcggcc ggggcgacgc tcgcggcaag cgcggcgcgt
     1681 tgggcgtcgt ccacgaggcc cgcgtcgtcc gtgttgaaca cgtagatgac gggcttggcg
     1741 gtcaggaggg tgagttcgcg aattgcggca agctccaggc ccgcggcctt cgcccccgcg
     1801 gagagcgtcg tgccaccatc gagaatcgtc cgcgccgtct cgcacgccgc gaggtagtcc
     1861 ggcgaggcct tcttcccgcg gacctccttc tcgatgcgcg gaatcgcctt atcgagcgtc
     1921 tgaaggtcgg cgaggatcaa ctcggtgttg atcgtctcga ggtccgacgc ggcgtcgacg
     1981 cggccctcga cgtggacgac gtcagggtcc gcgaaggccc gggtcacctg gcaaatcgca
     2041 tcggcctcgc ggatgtgcga caggaacgcg ttgcccaatc cctcgccctc ggatgccccc
     2101 ttgacgatcc ccgcgatgtc cacgaaactc accgtggcgg gcatgatcct ctccgagtgg
     2161 aagagctcag ag
//
LOCUS       DASVKP010000150         5125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1289975, whole genome shotgun sequence.
ACCESSION   DASVKP010000150 DASVKP010000000
VERSION     DASVKP010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289975"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1534
                     /locus_tag="VF362_03115"
     CDS             179..1534
                     /locus_tag="VF362_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX7589950.1"
                     /translation="MSDEQVVYTLDPDEEIKAWYNVVADLPTPPPPALHPGTREPAGP
                     DDLAPLFPMDLIMQEVSQERYIEIPEEVLNVYRQYRPSPLYRARNWEKKLGTPARIYV
                     KYEGVSPAGSHKVNTAVPQVYYNKISGITRLTTETGAGQWGTALAYACALFGVTCEVW
                     QVGASYDAKPQRRVLMEVFGAKVHRSPSRLTNAGRAFPEDHPGSLGIAISEAVEVAAP
                     DPGAKYALGSVLNHVLLHQTVIGQETLLQLAKAGEGAPDVVIACAGGGSNFAGLAFPF
                     LRENLVNGTNTRLVAVEPTSCPTMTRGEYRYDFGDTACTTPLFKMYTLGHDFVPSPIH
                     AGGLRYHGMAPLVSHVLHEGLMEAVALHQNECFEAGVEFARTQGIVPAPESSHALAQA
                     RREALAAKGSGEEKVIVVGLSGHGLLEISAYEQFLAGKLTDDALSESALAASLAAVPE
                     V"
     gene            1955..2473
                     /locus_tag="VF362_03120"
     CDS             1955..2473
                     /locus_tag="VF362_03120"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX7589951.1"
                     /translation="MFEMWIPFAGPPLVPAGDDAPLIRALLGTARCVGYACDARASGA
                     HMSAVQAQLAAQLARSPGGLPSSTLAERLGVTKQAITGLVDRMEYAGLVLRTEHPLDG
                     RCTLVRLTEHGASEFVGVARRLAAFNRELELRLGPDRDAQLKHLLWVITEGAADAGIG
                     CGSHGVGGTPEA"
     gene            complement(2501..2878)
                     /locus_tag="VF362_03125"
     CDS             complement(2501..2878)
                     /locus_tag="VF362_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589952.1"
                     /translation="MKRMALAATLGLVLVGCGPPPDFGPVSVYDPHGLHYEATIFEGT
                     IHITAGCMTVHWQGPDVVLLFPSDEVMWDPKAAAFTYGGRAYADGDAISLEAIVDFGR
                     TAGSVPEACPPSVRVYVPQAGLD"
     gene            2983..4110
                     /locus_tag="VF362_03130"
     CDS             2983..4110
                     /locus_tag="VF362_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7589953.1"
                     /translation="MGDLTEPRVFLDASGSAPMNARTREAFLAATDEGWADPERQHAE
                     SRRARALLDGAAQAIAGVLGAAAEHTHFVPTFGLAFDRAIGGVAKARTGRGLIVATAV
                     ERKPVLRMASRYSEAVTRVAVDAAGVVNLADFAGALNDTVSVALLQHANQETGVLQPL
                     AEAHEAARAVGVPLIVDATASVGHTDPPAAWDALMADPADWGGPRGVAVIAFRATTRW
                     VPLPPIVTGRVNVAAALAAAVALQEREEHRAEVAPRLAALAARIRAAAAELPDTVVFV
                     PTEPALPHLVTFACARIDGEALATELDRRGFAVGSGSACVTEPSPESHVWEALGAGGR
                     GVNARGVVRVGLPPGVIDQDVDRFIVALRDAVAALVRGAGL"
     gene            4320..4982
                     /locus_tag="VF362_03135"
     CDS             4320..4982
                     /locus_tag="VF362_03135"
                     /inference="COORDINATES: protein motif:HMM:NF044737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589954.1"
                     /translation="MDEDEISLDDLFHRHTMPLDHQIALKTALYRLTRKFSSMFTEHT
                     IEGFLTGSYEQYAGLSRVPHFVHILAERFAFERLTAFAVAEHHLTSGQPIVVFVCQTN
                     DLLSPMARGLFMKHAGAGALAWSAGLEPALALRPGARSSLQEIGVDISKDFPKPLALE
                     MLQAASTVVGFGCIDRLPRLPGREYLDADAPSPVPGDEESVRQIRDWLNEQTAELLAR
                     LT"
BASE COUNT          753 a         1888 c         1731 g          753 t
ORIGIN      
        1 gaactctcct tggggtgggg aacggccttc acaagagata tcgcccgcgc ggcgagaaag
       61 gttacgcggt ccgcccaact tgcccgccct cccctcgccc gatacgctcc gaggacggcc
      121 gagggcttgc cgacgacgaa cgcgacatca ggaccaccac cgcagcaagg gagtagcgat
      181 gagcgacgaa caggttgtgt acaccctgga tcccgacgaa gagatcaagg cctggtacaa
      241 cgtcgtcgcg gacctgccca cgccgcctcc tccggcgctg caccccggca ccagggagcc
      301 cgcgggccct gacgacctcg cccccctgtt cccgatggac ctgatcatgc aggaggtctc
      361 ccaggagcgg tacatcgaga tccccgagga ggtgctcaac gtctaccgtc agtaccgtcc
      421 gtccccgctc taccgcgcgc ggaactggga gaagaagctc ggcaccccag cccgcatcta
      481 cgtcaagtac gagggcgtct cccctgccgg ctcccacaag gtcaacaccg ccgttccgca
      541 ggtgtactac aacaagatca gcggcatcac gcgcctcacc accgagaccg gcgcgggcca
      601 gtgggggacc gccctcgcct acgcgtgcgc cctgttcggc gtcacgtgcg aggtgtggca
      661 ggtgggcgcc agctacgacg ccaagccgca gcggcgggtg ctcatggagg tgttcggcgc
      721 caaggtgcac cgctcgccct cgcgcctcac caacgccggc cgcgccttcc ccgaggacca
      781 ccccggctcg ctcggcatcg ccatctccga ggccgtggag gtcgccgcgc cggaccccgg
      841 ggcgaagtac gccctgggct ccgtcctcaa ccatgtgctg ctccaccaga ccgtcatcgg
      901 ccaggagacg ctccttcaac tcgccaaggc tggggagggg gcgcccgacg tcgtgatcgc
      961 ctgtgcgggc ggcggctcca acttcgcggg cctggccttc ccgttcctgc gcgagaacct
     1021 ggtcaacggc accaacacgc gcctggtcgc ggtcgagccg acgtcgtgcc ccacgatgac
     1081 gcgcggcgag taccgctacg acttcggcga caccgcctgc accacgcccc tgttcaagat
     1141 gtacacgctg gggcacgact ttgtgccgtc gcccattcac gccggtggct tgcgctacca
     1201 cggcatggca ccgctggtct ctcacgtgct ccacgaggga ctcatggagg ccgtcgccct
     1261 gcaccagaac gagtgcttcg aggcgggcgt cgaattcgcg cgcacccagg gcatcgtccc
     1321 agctcccgag tcgtcccacg cgctcgcgca ggcgcgccgc gaggcgctcg ccgccaaggg
     1381 atcgggcgaa gagaaggtca tcgtcgtcgg gctgtccggg cacgggttgc tcgagatctc
     1441 ggcgtacgaa cagttcctcg ccggcaagct cacggacgac gcgctgagcg agtccgcgct
     1501 ggccgcgtcc cttgcggcgg tgccggaggt ttaggagaag ccggatctag ttcgcgaacc
     1561 gttgacgccg ggaccgttga cggcgggacc gctcaccccg ggaccattcg tgcgcgagtt
     1621 cgtcaacgcg ttgactgatg cgaacacgtg gaccatcttg ccgagaccgt tcaccccgga
     1681 agcgttcacg gcgggaccgc tcacggcggg ccacgtcggc cgcgcccgac gtcgtccgcg
     1741 agcgtcgcgc ccagcgtcgt ccgctggcgt cgtccgctgg cgtcgtccgc gagcatcgtc
     1801 cccagcccgc ggacaggcct ccggcctccc caggacacac gtcgcccccg gccgccgccc
     1861 ccagcgtcgt ccccagcccg tggacgggcc cccgcctccc ccggccgggg ccgcgccgac
     1921 agcgcacccg cccacatcgc tcaacgtgtt gaccatgttc gaaatgtgga ttccctttgc
     1981 cgggccgcca ctcgtgcccg ccggcgacga cgccccgttg attcgcgctc tgctcgggac
     2041 cgcgcgatgc gtcggatacg cgtgcgacgc gcgcgcgagt ggtgcgcaca tgagcgccgt
     2101 gcaagcgcaa ctcgccgcgc aactcgctcg ttcccctgga ggactcccgt cgtcgacgct
     2161 tgccgagcga ctcggggtga ccaagcaagc catcacggga cttgtggatc gcatggagta
     2221 cgccggactg gtcctgcgca cggagcatcc actcgatgga aggtgcacgc ttgtcaggct
     2281 gaccgagcac ggggccagcg agttcgtggg agtcgcgcgg cggctggccg cgttcaaccg
     2341 cgagctcgaa ttgaggctcg gacccgaccg ggacgcacaa ctgaagcacc tcctgtgggt
     2401 gatcaccgag ggagcggcgg acgccggtat tgggtgtggg agccatggag tggggggcac
     2461 gccagaggct taggagaacc cagatctagt caagtcccgt ctagtcaagt cccgcctggg
     2521 ggacgtagac gcgcacggag ggcgggcacg cctcggggac acttccggcg gtcctgccga
     2581 agtcgacgat ggcctccagg ctaatggcgt cgccgtccgc gtacgccctg cctccgtagg
     2641 tgaacgccgc ggccttcggg tcccacatca cctcgtccga ggggaagagg agcacgacgt
     2701 cggggccctg ccagtgcacc gtcatacagc ccgcggtgat gtggatcgtg ccctcgaaga
     2761 tggtcgcttc gtagtggagt ccgtgcgggt cgtacaccga gacgggcccg aagtcggggg
     2821 gcgggccgca gccgacgagt accaggccga gggtggcggc aagcgccatt cgcttcatgg
     2881 ggccaggatg gcacagacgg cggcggggcg acgcccgcgg cccgagcgcg ggatgcgcgg
     2941 ttgaggcgcg cgcacggcgc ctcgcccgct aggttggtgc gtatgggtga cctaaccgag
     3001 ccgcgcgtgt tcttggacgc ttccgggtcc gccccgatga acgcgcggac gcgcgaggcg
     3061 ttcctcgctg cgacggacga gggttgggcc gacccggagc gccagcacgc ggagtcccgg
     3121 cgggcgcgcg cgctgctcga tggtgccgcc caggcgatcg cgggcgtcct cggggcggcc
     3181 gccgagcaca cccacttcgt cccgacgttc gggctggcgt tcgaccgcgc gatcggcggc
     3241 gtcgcgaagg cccggaccgg ccgggggctc atcgtcgcga ccgcagtcga gcgcaagccg
     3301 gtcctcagga tggcctcccg ctactccgag gcggtcacgc gcgtcgcggt ggacgccgcg
     3361 ggagtggtca acctcgcgga cttcgcgggc gccctcaacg acacggtctc cgtggcgctc
     3421 ctccagcacg ccaatcagga gacgggcgtc ctccagccgc ttgccgaggc ccacgaggcc
     3481 gcccgcgccg tcggcgttcc cctcatcgtc gacgcgaccg cgagcgtcgg gcacaccgac
     3541 ccgcccgccg cgtgggatgc gctcatggcg gaccccgcgg actggggcgg tccgcgcggc
     3601 gtcgccgtca tcgcgttcag ggcgacaacg cggtgggtgc ccctgccccc gatcgtgacg
     3661 ggccgcgtca acgtggcggc ggcgcttgcc gcggccgtgg cgctccagga gcgggaggaa
     3721 catcgcgccg aggttgcccc ccgcctcgcg gcgctcgccg cccggatccg cgccgccgcg
     3781 gcggaactcc ccgacaccgt cgtcttcgtc ccgaccgagc ccgcgctccc ccacctcgtc
     3841 acgtttgcgt gcgcgcggat cgacggggag gcgctggcca cggagctgga ccggcgcggg
     3901 ttcgccgtcg gctccggctc ggcctgcgtc acggaaccgt cgcccgagag tcacgtgtgg
     3961 gaggcgctcg gcgcgggtgg gcgcggcgtg aatgcacgcg gcgtggtccg cgtggggctt
     4021 ccccccggcg tcattgacca agacgtggac cgcttcatcg tggcgcttcg cgacgccgtt
     4081 gccgcgctcg ttcgcggggc gggcctctag ttggagatgg tcaacacgtt gaccataggg
     4141 ggctccgcgc gacgccaccg ccatgaccgg gaactcgagg gcgccaggcc gctgagcgcg
     4201 ccgctctttg cgtagttcgt cccggcgcgt ccggccccag gatgaacgtt tggttacgcg
     4261 agggccaaac tcgtatgatc gcgccgcagg ccctgcgatc ctgaggggcg tgaacgcgca
     4321 tggatgaaga cgagatctcc ctcgacgacc tcttccatcg ccacacgatg ccgctcgacc
     4381 accagatcgc gctcaagacg gcgctctacc gcctcacccg caagttctcg tcgatgttca
     4441 ccgagcacac gatcgaaggc ttcctcaccg gctcgtacga gcaatatgca gggttgtccc
     4501 gggtcccgca cttcgtccac atcctcgcgg aacgcttcgc cttcgagcgc ctcaccgcct
     4561 tcgccgtcgc cgagcaccac ctcacgagcg ggcaacccat cgtcgtgttc gtgtgccaga
     4621 cgaacgacct cctttccccc atggcgcgcg ggctgttcat gaagcacgcg ggcgcgggcg
     4681 cgctcgcctg gtccgcgggg ctcgagcccg ccctcgccct ccggcccggg gcgcgctcgt
     4741 cgctccagga aatcggcgtc gacatctcca aggactttcc caagcccctc gccctggaga
     4801 tgctccaggc cgcctcaacc gtcgtcggat tcggctgcat cgaccgcctc ccccggctcc
     4861 cgggcaggga gtacctggac gccgacgccc cgagcccggt gcccggcgac gaggagtcgg
     4921 tccggcagat ccgggactgg ctgaacgagc agacggccga acttctcgca cggttgacct
     4981 agggcgcccg gccctccgcg ccgtggacac cttctcgcaa ccttcggggg ctagcgtcac
     5041 gagcgtgtcc actgaaactc tcccccggtt cttcgccgac gcggtcggcc aggcccccct
     5101 cgaccccatt gcccgcgagg tctac
//
LOCUS       DASVKP010000151         1817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_713390, whole genome shotgun sequence.
ACCESSION   DASVKP010000151 DASVKP010000000
VERSION     DASVKP010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713390"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VF362_03140"
     CDS             complement(<1..749)
                     /locus_tag="VF362_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7589955.1"
                     /translation="MTSTTTAPTVIDPALAATMRHGYKDYGKGETAVRALDGISVDIK
                     RSEFTAIMGPSGSGKSTLLHTIAGLDFLTAGNTFIGDIDLTTLSEAQVTQVRRDHIGF
                     VFQSYNLIPSLTAAENIRLPLDIAGRKVDEDWYNQIVEVLGLGDRLHHLPAELSGGQQ
                     QRVAVARALVSKPEIIFADEPSGNLDSKSSSELLGFMRRAVREFKQTIVMVTHDAGAA
                     SYSDRILFLEDGKIVDEMREPTASKVLDRMK"
     gene            complement(880..1557)
                     /locus_tag="VF362_03145"
     CDS             complement(880..1557)
                     /locus_tag="VF362_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7589956.1"
                     /translation="MIRVLLVDDQAMVRAGLRMILDSEPGIEVVGEVDDGAGAAAAVA
                     ELRPDVILMDIRMPGMDGLAATREVIAAHPDAKVVVLTTFDLDEYVYGALRAGASGFL
                     LKSASGDALVNAVKVVAGGEALLAPEVTRRVIEEFARATRDVGTGGVEHVVPADAIGD
                     LSEREVEVLQLMAQGMSNQEIAKDLYLSSTTVKTHVSHILTKLGVRDRVQAVVEAYDS
                     GIVAPRG"
     gene            complement(1554..>1817)
                     /locus_tag="VF362_03150"
     CDS             complement(1554..>1817)
                     /locus_tag="VF362_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7589957.1"
                     /translation="TITVEDDGRGVISDATKGGSGHGLAGMRERVATLGGSIELGPRP
                     GGGYRVRAVLPSQDDVVNTPSRERKDSIAAIISLVEASERKNA"
BASE COUNT          266 a          600 c          631 g          320 t
ORIGIN      
        1 ttcttcatgc ggtcgagtac cttcgaggcg gtcggctcgc gcatctcgtc gacgatcttt
       61 ccgtcctcaa ggaagaggat gcggtcggag tacgacgcgg ctcccgcgtc gtgggtgacc
      121 atcacgatgg tctgcttgaa ctcgcggacg gcgcggcgca tgaacccgag cagttcggaa
      181 ctggacttgg agtcgaggtt cccggacggc tcgtcggcaa agatgatctc gggcttggac
      241 accagcgcgc gcgcgacggc gacgcgttgc tgctgtccgc cacttagttc cgcgggaagg
      301 tggtggagcc ggtcgccgag ccccagaacc tcgacgattt ggttgtacca gtcttcgtcc
      361 accttgcggc cggcgatgtc gagcggcagg cgaatgttct ccgccgccgt cagcgagggg
      421 atcaggttgt acgactggaa gacgaatccg atgtggtctc ggcgcacctg cgtgacctga
      481 gcctcgctca gcgtggtgag gtcgatgtcg ccgatgaagg tgttgcccgc cgtcagaaag
      541 tcgaggcccg cgatggtgtg caggagcgtg gacttgcccg agcccgaggg gcccatgatc
      601 gcggtgaact cggagcgctt gatgtcgact gagatgccgt cgagcgcgcg aacggcggtc
      661 tcgcccttgc cgtagtcctt gtagccgtgg cgcatggtcg cggcgagcgc ggggtcgatg
      721 acggtcggcg ccgtggtggt gctggtcatg atgtgccttt cgagtgtggt gtcgatgcgc
      781 ccagtcttcc gggaggcgcg acggggcgat atcgcccgcg aggatggttt ggcgggatcg
      841 tcctgaggga tgaccctggg gaccggagga ggagggctcc tagccccgtg gggcgacgat
      901 tccggagtcg tacgcctcga cgaccgcctg cacgcggtca cggaccccga gcttcgtcag
      961 gatgtggctc acgtgcgtct tgaccgtcgt ggacgagagg tacaggtcct tggcgatctc
     1021 ctggttagac atcccctgcg ccatcagttg aaggacctcg acctcccgtt cgctaaggtc
     1081 gccgatcgcg tcggcgggaa cgacgtgctc aacgcctccg gtcccgacgt cccgcgtcgc
     1141 gcgggcgaac tcctcgatca cccggcgcgt cacctcgggg gcgaggagcg cctcaccccc
     1201 cgccacgacc ttgacggcgt tgacgagcgc gtcaccgctc gcgctcttga gcaggaagcc
     1261 cgacgccccc gcacggaggg cgccgtagac gtactcgtcc aggtcgaagg tcgtcagcac
     1321 gacgaccttc gcgtcggggt gcgccgcgat cacctcgcgc gtcgcggcga gcccgtccat
     1381 gccgggcatg cgaatgtcca tgaggatcac gtccggccgg agctcggcga cggcggccgc
     1441 ggcgcccgcc ccgtcgtcga cctcacccac gacctcgatg cccggctcgg agtcgaggat
     1501 catacgaagc ccggctcgca ccatggcctg atcatcgacg agtagcaccc tgatcacgcg
     1561 ttcttccttt cgctcgcctc gacgagggag atgatggccg cgatcgagtc cttccgctcg
     1621 cggctcggag tgttgaccac gtcgtcctgg gagggcagga ccgcgcgaac ccggtagccg
     1681 ccgcccgggc gcgggccgag ctcgatcgag ccgcccaggg tcgcgaccct ctcccgcatt
     1741 cccgccaggc cgtggccgct accccccttg gtcgcgtcgc taatgacgcc gcggccgtcg
     1801 tcctccacgg tgatggt
//
LOCUS       DASVKP010000152         3673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1041513, whole genome shotgun sequence.
ACCESSION   DASVKP010000152 DASVKP010000000
VERSION     DASVKP010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041513"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /gene="hisE"
                     /locus_tag="VF362_03155"
     CDS             complement(<1..160)
                     /gene="hisE"
                     /locus_tag="VF362_03155"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017489614.1"
                     /note="catalyzes the formation of 1-(5-phosphoribosyl)-AMP
                     from 1-(5-phosphoribolsyl)-ATP in histidine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphatase"
                     /protein_id="HEX7589958.1"
                     /translation="MKSFDDLYQELSRRAIERPAGSRTVELLDAGVHAIGKKLVEEAA
                     EAWMAAEFQ"
     gene            complement(171..668)
                     /gene="ribH"
                     /locus_tag="VF362_03160"
     CDS             complement(171..668)
                     /gene="ribH"
                     /locus_tag="VF362_03160"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX7589959.1"
                     /translation="MTGAGAPGLAVDGAGMTVEIVAASWHAEVMDGLVAGAAGACEAA
                     GASYRITRVPGCFEIPVVAQALAARGAVAIVGLGVVIRGDTPHFEYVCDAVTQGLTDV
                     ARINEVAVGFGILTCDSDAQAIARAGLPGSKESKGAEATDAAIATARILENLSSSPRV
                     GVRLP"
     gene            complement(665..1921)
                     /gene="ribA"
                     /locus_tag="VF362_03165"
     CDS             complement(665..1921)
                     /gene="ribA"
                     /locus_tag="VF362_03165"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX7589960.1"
                     /translation="MSKDRALKDSLALVDAALADLRLGKPVLVADSRYRENEADFILS
                     AQTTTVEWVAWAIRHSSGYLCAPMPAERADALALPLMVPLSQDPRRTAYTVSVDAASG
                     VTTGISAADRHTTLKALAKPDATSHDVIRPGHVLPLRAVPGGVLHRPGHTEACVDLCR
                     LAGLEPVGALAELVNDDGTMMRLADAGLLADQEGLVLLSITDIIKYRRHHGDLPAEAY
                     VQPHRVTFSGEAQLPTEHGDVMVRGYRDERTGDELVALVAPTRPGVIPMVRVHSECLT
                     GDAFGSLRCDCGPQLDSAIEAVVREGGAVVYVTGHEGRGIGIINKIQAYSLQDLGRDT
                     VDANAELGLPVDRREYGGAAAILHELGMTSVRLLTNNPAKVEGLRASGIEVVERVPHH
                     VGGNPHNERYRQTKVDRMGHLEDADE"
     gene            complement(1918..2511)
                     /locus_tag="VF362_03170"
     CDS             complement(1918..2511)
                     /locus_tag="VF362_03170"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX7589961.1"
                     /translation="MFTGIVESMGTITSVDESGAQAKITISSPGFGDDLQHGESVAVS
                     GVCLTVAQHAGGSWVADVMRVTRETTTLGEAVPGWRVNLERALRAGGRMGGHIVQGHV
                     DGIATLVRRDSAAEWDDLTFSVPKDVARYVVTKGSICLDGVSLTVAALDGRDATVSLI
                     PTTLADTTLGALSVGDRVNVEVDVLAKYIERFVGGAA"
     gene            complement(2517..3506)
                     /gene="ribD"
                     /locus_tag="VF362_03175"
     CDS             complement(2517..3506)
                     /gene="ribD"
                     /locus_tag="VF362_03175"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX7589962.1"
                     /translation="MARAVELSIQGPEYGPNPRVGCVILDARGAVVGEGFHRGAGTPH
                     AEVEAISDAARRGNSTRGATAYVTLEPCRHVGRTGPCVDALTEAGVARVVYAVPDPGE
                     TSGGGADVLRERGIEAIPQPTPAAEEVNARFLHSLTTGRPYVILKFACTLDGRTAAAD
                     GTSFWITGQEARDHAHRERGRVDAIIVGTGTVVTDDPELSARPGGIEGGHQPLRVVMG
                     ARETSGRKIWRDDNAMQCWTHDPRVVLEALQEREVRSVIVEGGATISSAFLRSGLVDE
                     VHAYLAPVLLGAGRGVVTGLGIRSIGEALRLDDVRTVSLGPDTLVTGRLQPRG"
BASE COUNT          541 a         1303 c         1286 g          543 t
ORIGIN      
        1 actggaactc cgcggccatc caggcctcgg ccgcctcctc cacgagcttc ttgccgatgg
       61 cgtggacgcc ggcgtcgagg agttcaacgg tgcgcgaacc cgcgggccgc tcgatggcgc
      121 ggcgcgaaag ttcctggtac aggtcgtcga agctcttcac gacgattagc ctacgggagg
      181 cggacgccca ctcggggcga ggaggagagg ttctcgagaa ttcgcgccgt cgcgattgcg
      241 gcatcggtcg cctcggcacc cttgctttcc ttcgatcctg gcaggcctgc gcgcgcgatc
      301 gcctgggcgt cggaatcgca ggtgaggata ccgaagccca ccgcgacctc gttgatcctc
      361 gcgacgtccg tcagcccctg cgtgacggcg tcgcacacat actcgaagtg gggcgtgtcg
      421 ccgcggatga cgacgccgag gccgacgatg gcgacggccc ctcgagccgc aagggcctgc
      481 gccacgaccg ggatctcgaa gcagccggga acgcgggtga tccgatacga cgccccggcg
      541 gcctcgcatg cgcccgcggc accagctacc aggccgtcca tgacctcggc gtgccacgac
      601 gcggcgacga tctcgaccgt catgcccgcg ccgtcaacgg cgaggccggg cgctcccgct
      661 ccggtcactc gtcggcatcc tcaaggtggc ccatgcgatc caccttagtc tgacggtagc
      721 gctcgttgtg ggggttgccg cccacgtgat ggggcacgcg ctcgacgacc tcgatcccgc
      781 tcgcgcggag gccctcgacc ttcgcgggat tattcgtcag cagccggacc gacgtcatgc
      841 cgagttcgtg gaggatcgcg gccgcgccgc catactcgcg gcggtcgacg ggcaggccca
      901 gctcggcatt cgcgtcgacc gtgtctcgcc ccaggtcctg gagggagtag gcctggatct
      961 tgttgatgat cccgattccc cgcccctcgt gtcccgtgac gtagacgacc gcgccgccct
     1021 cgcgcacgac cgcctcgatc gcggagtcca gctgggggcc gcagtcgcaa cggagggaac
     1081 cgaaggcgtc acccgtgagg cactccgagt ggacgcgcac catggggatg acgcccggcc
     1141 gcgtgggcgc gacgagcgcg accagttcgt cgccggtccg ctcgtcgcgg tacccgcgca
     1201 ccatgacgtc gccgtgctcg gtcgggagct gggcctcgcc gctgaaggtc acgcggtgcg
     1261 gctgaacgta ggcttccgcg ggcagatccc cgtggtggcg gcggtacttg ataatgtcgg
     1321 tgatactcaa gagaacgagg ccttcctggt cggcgagcaa gcccgcatcg gccaggcgca
     1381 tcatggtgcc atcgtcgttg accagttccg cgagcgcccc gacgggctcg aggcccgcga
     1441 ggcggcagag gtcgacgcag gcttccgtgt ggccgggtcg gtgcagtacg ccacctggaa
     1501 cggcgcggag gggcaaaaca tgcccgggtc ggatcacgtc gtgggaggtc gcgtccggtt
     1561 tcgcgagggc cttgagggtc gtgtgcctgt ccgcagccga gattccggtc gtgacgccag
     1621 aggccgcgtc caccgagacc gtgtacgcgg tgcggcgggg gtcttggctg aggggcacca
     1681 tcaggggaag cgccagcgcg tcggcgcgct ccgcgggcat cggggcgcag aggtacccgc
     1741 tcgagtgccg gatcgcccac gccacccact cgaccgtcgt cgtctgggcg gacaggatga
     1801 agtcggcctc gttctcgcga taccgcgagt ccgccaccag cacgggtttg ccgaggcgga
     1861 gatccgcgag cgcggcgtcg acgagcgcga gcgaatcctt gagcgcgcgg tccttgctca
     1921 cgcggctccg ccgacgaacc gctcgatgta cttggcgagc acgtcaacct cgacgttgac
     1981 gcggtctccg accgagaggg cgccgagggt ggtgtcggcg agggtcgtcg ggatgagcga
     2041 caccgtcgcg tcgcgtccat caagggcggc gacggtaagg gagacgccgt cgaggcagat
     2101 cgagcccttc gtcacgacgt agcgcgcgac gtccttgggg acagagaagg tcaggtcgtc
     2161 ccactcggcg gcgctatcgc ggcgcacgag cgtcgcgatg ccgtcgacgt ggccctggac
     2221 gatgtgaccg cccatccggc cgcccgcgcg aagggcccgc tcgaggttga cgcgccagcc
     2281 ggggaccgcc tcgcccaggg tggtggtctc gcgggtgacg cgcatgacgt ccgccaccca
     2341 tgagccgccc gcgtgctgcg cgaccgtcag gcacactccg gagacggcga cggactcgcc
     2401 gtgctggaga tcgtcgccga agccgggcga ggagatcgtg atcttggcct gggccccgga
     2461 ctcgtccacg gacgtgatcg tccccatgga ttccacgatg ccggtgaaca tgggccctat
     2521 cctctcggtt gcagtcgtcc cgtcaccagg gtatccgggc cgagcgagac ggtacgcaca
     2581 tcgtccaagc gcaacgcctc cccgatgctg cggattccca gtcccgtgac gaccccccgt
     2641 cctgcgccga ggaggaccgg cgcaaggtac gcgtgcacct cgtccaccag ccccgagcgg
     2701 aggaaggcgg aactaatggt cgcacccccc tcaacgatca ccgagcgaac ctcgcgctcc
     2761 tggagcgcct ccagcaccac ccgcgggtcg tgggtccagc actgcatcgc attgtcatcg
     2821 cgccagatct tcctgccgga cgtctcgcgc gcgcccatca ccacacgaag gggctgatgc
     2881 cccccctcga tcccgccggg cctcgcggag agttcggggt cgtcagtcac caccgttccc
     2941 gtcccgacga tgatcgcgtc caccctgccc cgctcccggt gcgcgtgatc cctggcctcc
     3001 tgcccggtga tccagaagct cgtgccgtcc gccgccgccg tgcggccgtc gagcgtgcac
     3061 gcaaacttga ggatgacata cgggcgaccc gtcgtcagcg aatggaggaa gcgcgcgttg
     3121 acctcctcgg cggcgggcgt gggctggggg atcgcctcga tcccgcgctc ccggaggacg
     3181 tccgcgcccc cgcccgaggt ctctcccgga tcgggcacgg cgtagacgac gcgcgcaacg
     3241 cccgcctcgg tgagggcgtc gacgcacggc ccagtccgcc cgacgtgacg gcacggttct
     3301 agggtcacgt aggcggttgc gccccgagtc gaattgccgc gacgtgccgc atcggagatt
     3361 gcctcgacct ccgcgtgggg ggtccccgcg ccgcgatgga agccctcgcc gaccaccgcg
     3421 ccgcgggcat cgaggatgac gcagccgact cgggggttcg gcccgtactc gggaccctgg
     3481 atggaaagct cgacggctcg cgccatcgcg gcccccgggg ttagggccgc cggggtcagg
     3541 gtcgccgggt tcagagacgc cgcgccggac ggctcggccg cgctagccgt ggtggccccc
     3601 cgctgacaac gccttgaggg cggcgatctc ggcgcccctg ttctcggccc catacacggc
     3661 cgagccaagg aca
//
LOCUS       DASVKP010000153         2610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_467547, whole genome shotgun sequence.
ACCESSION   DASVKP010000153 DASVKP010000000
VERSION     DASVKP010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467547"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2066
                     /locus_tag="VF362_03180"
     CDS             <1..2066
                     /locus_tag="VF362_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589963.1"
                     /translation="SLSYPGFAGGMIAKFLSSSTADGHNPYRVLREGFEWEVADSDEP
                     WASIGYWGDHQVIYLLRLLESAQRHRPGSLKHLLTRPVFTYADVPYRIRPYASLLDDP
                     HETIDFDAEASAAALDRAARQGSDGLALLGADGAVVRANGSEKLLVVALAKLANFIPE
                     AGIWLNTQRPEWNDGNNALVGNGVSVVTLCYLRRYLAFVKDLFATIEGGGAEVAAEVA
                     LFLTEVRSALEAHAGSLGGAISDRDRRALMDALGEAGSRYREGIYAAGFSGGRTTVTA
                     DALTGFCDLALRHIDHSIRVNRRADGLYHSYNLMRIVDDGVEFRHLQEMLEGQVAVLS
                     SGVLAIEEAIALLDALRASDLYRADQASYILYPNRDLPRFLDKNLVPSEAVERSALLR
                     AMLAEGDTSVVERDVDGDVHFHASFSNAGFLSQALDRLESGAHGDAARAERGRILDVY
                     EEVFDHRSFTGRSGTFYKYEGLGSVYWHMVSKLLVAVDEVWVDALSRGVDAARVARLA
                     AHYREIREGIGVHKSPSDYGAIPTDPYSHTPAFAGAQQPGMTGQVKEDLISRFSDMGV
                     VVADGRIRFVPERVNGEFLETPGALDYVAADGTEARLDLEVGTMGYTLCQVPVVLHRS
                     GDPRVEVTRVDAKPVETTAVKTSAVGTTGLVTTGLVLGEAASAAVFDRSGEIARFDVF
                     LGAVD"
     gene            2381..>2610
                     /locus_tag="VF362_03185"
     CDS             2381..>2610
                     /locus_tag="VF362_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589964.1"
                     /translation="MSEDITTPVSTASIFQAPEPAAPRVSRRATAPAGPPIIPVDDAP
                     APKAKRKPATKKAAPNKAVESKAEAEGEPKKKA"
BASE COUNT          426 a          901 c          876 g          407 t
ORIGIN      
        1 tctcgctctc gtacccgggc ttcgccgggg gcatgatcgc caagttcctc agttcgtcca
       61 ccgccgacgg gcacaacccc taccgcgtac tgcgcgaggg attcgagtgg gaggtcgccg
      121 attccgacga gccgtgggcc agcatcggct actggggaga ccaccaggtc atctacctcc
      181 tccgcctcct ggagtcggct cagcggcacc gccctggcag cctcaagcac ctgttgacgc
      241 gtccggtctt cacctacgcg gacgttccgt atcggatccg cccctacgcg agcctgctgg
      301 atgatcccca cgagacgatc gacttcgacg ccgaggcgag cgccgcggcg ctcgacaggg
      361 ccgcgcgcca ggggtcggac gggctcgccc ttctcggcgc ggacggagcc gtggttcgcg
      421 cgaatggcag cgagaaactc ctcgtcgtcg cgctggccaa gttggcgaac ttcatccccg
      481 aggcaggaat ctggctcaac acccagcgcc ccgaatggaa cgacgggaac aacgccctgg
      541 tcggcaacgg cgtctccgtg gtgacgctct gctacctgcg gaggtatctc gccttcgtga
      601 aggatctctt cgcgaccatc gaggggggcg gggcggaggt cgccgcggag gtcgccctct
      661 tcctgaccga ggtccgttcc gcgctcgagg cgcacgccgg ctcgcttgga ggcgccatct
      721 ccgatcgcga tcggcgggcg ctcatggacg cgctcggcga ggcgggctcc cggtaccgcg
      781 agggcatcta cgccgcgggc ttctccggcg gtcggaccac ggtcaccgcc gacgcgctca
      841 ctgggttctg cgacctcgcg ttgcgccaca tcgatcactc gattcgggtg aaccgcaggg
      901 ccgacggcct ctatcactcc tacaacctga tgcggatcgt ggacgatggc gtcgagtttc
      961 ggcatctgca agagatgttg gagggccaag tcgcggtgct gagttccggc gtcctggcca
     1021 tcgaggaggc gatcgccctg ctcgacgccc tccgggcgag cgacctgtac cgcgcggacc
     1081 aggcaagtta catcctgtat cccaaccgcg acctcccacg atttctcgac aagaacctcg
     1141 tcccgagtga ggcggtcgag cggtccgcgc tgctccgcgc gatgctcgcg gaaggtgaca
     1201 cctcggtcgt cgagagggac gtggacggcg acgtgcactt ccacgcctca ttcagcaacg
     1261 cgggattcct gagccaggcc ctggatcggc tcgaatccgg ggcccatggc gacgctgcgc
     1321 gcgccgagcg cggacggatt ctcgacgtct acgaagaggt cttcgatcac cgctccttca
     1381 cgggacgctc gggaaccttc tacaagtacg agggcctcgg ctccgtctac tggcacatgg
     1441 tctcgaagct tctggtcgcc gttgacgagg tgtgggtcga cgcgctctcg cgcggcgtcg
     1501 acgccgccag ggtcgcgcgg ctcgcggcgc actatcgcga gattcgcgag gggatcggcg
     1561 tccacaagtc gccatcggac tacggcgcga tcccgacgga cccctattcc cacacccccg
     1621 cgttcgcggg cgcgcagcaa ccgggcatga cgggtcaggt gaaggaggac ctcatttcgc
     1681 gcttctccga catgggggtc gtggtcgcag acgggcgtat ccgcttcgtc cctgagcggg
     1741 tgaacgggga gttccttgag acgccggggg ctctcgacta cgtcgccgcc gacgggaccg
     1801 aggcgcgcct cgatctggaa gtcggaacga tgggatacac gctgtgccag gtgcccgtcg
     1861 tccttcatcg ctcgggcgat ccccgcgtcg aggtgacgcg cgtcgacgcg aagcccgtcg
     1921 aaaccaccgc cgtcaagacc tcggccgtcg gaaccacggg cctcgtaacc acgggcctcg
     1981 ttctgggcga ggcggcaagc gccgcggtct tcgatcgctc cggcgagatc gcccggttcg
     2041 acgtcttcct tggcgcggtc gactagcgct agaagagccg ggtgcgggcg agcgcgggat
     2101 aagcggaccg tccggtctgc ggtgtgtcac aatggtccca ggtccgcaca cgtccacacc
     2161 gtgtgccaac cgtgcgacag aggcagcgac cgagtcggcc gcccgtcccg gccggatctc
     2221 cgcggacata tgtacgcgtg ggagatatcc ggggacgcga gcccgacaac gtggcgccgc
     2281 tgtccgccgc gaccgacaga cttctaagcc agccagcgag cgggcttcga ccgacaatac
     2341 gccgggtgga gtgtggccac accgacgcga ggtacccgcc ttgagcgagg acatcactac
     2401 acccgtatct accgccagca tcttccaggc gcccgaaccc gccgcgccgc gggtaagtcg
     2461 gcgcgcgacc gcgcccgccg ggccaccgat cattccggtg gacgacgcgc cagcgcccaa
     2521 ggcgaagagg aagcccgcga ccaagaaggc tgcgcccaat aaggcggtgg agtccaaggc
     2581 cgaggccgag ggcgagccca agaagaaggc
//
LOCUS       DASVKP010000154         5050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_713562, whole genome shotgun sequence.
ACCESSION   DASVKP010000154 DASVKP010000000
VERSION     DASVKP010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713562"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="VF362_03190"
     CDS             <1..1461
                     /locus_tag="VF362_03190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX7589965.1"
                     /translation="YGIQAAAREYFNVNAADLTVSQAALLAGIIPAPSAWDPRVDPVQ
                     AEARWNYVLDGMVTEGWLTPSNRLRLDFPTTAEYRKNNVFGGPQGYLLRDAIVEVAAR
                     TGLSQDAVETGGYTIVTSIDWATQQAAQNAAATLPEDHSPNLRLAIVTLDPTTGAITS
                     MYGGADYVTIQRDAVTQDIAQAGSTFKPFALIAALENGIGLESVYSGASPQAIAGFTK
                     PVKNFGNENFGNITLTTATEHSVNTVYAQLARDVGPDKVVDAAVRAGLPADTAGLEAN
                     PANVLGTASPHPLDMAAAYATIAAQGVRHDPFLVGTVKDATGGVVYAHELKEKREFSA
                     AVMADTTFALQQVVLHGSGSYAARLSRPIAGKTGTSSDNKSAWFIGFTPQIVGAVAMY
                     QVGADGSVEAITPFGGFKQITGGSVPCHIWTAMMGEVLPNLPVEQFPPRSHVGTAIVP
                     SPSPSPSVTPSPTPTPSASPTSGGGVSPSPSLSPSP"
     gene            1615..1905
                     /gene="rpsF"
                     /locus_tag="VF362_03195"
     CDS             1615..1905
                     /gene="rpsF"
                     /locus_tag="VF362_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX7589966.1"
                     /translation="MRKYEMMVILDPEVEERTVQPSLDQYLTVVKDDSGTVDRIDIWG
                     RRRLAYPIKKRTDGIYAVVQFTSEAATAKELERQLSLNETVLRTKLIRPDAH"
     gene            1933..2427
                     /locus_tag="VF362_03200"
     CDS             1933..2427
                     /locus_tag="VF362_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX7589967.1"
                     /translation="MAGDTTITVVGNLTADPELRFIQSGAAVVNFTVASTPRTFDRAT
                     NEWKDGETLFLRCSLWREAAENVAESLTKGMRVIVSGRLVQRSFESSGEKRTVVELQV
                     DEVGPSLRYATAKVTRTQRAGGGGAGGGGGGSGFSGASSSAPAGGSDSDPWASAPPSD
                     EPPF"
     gene            2503..2742
                     /gene="rpsR"
                     /locus_tag="VF362_03205"
     CDS             2503..2742
                     /gene="rpsR"
                     /locus_tag="VF362_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013350683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX7589968.1"
                     /translation="MAKTDLRKPRRKTNPLKAAKVVRVDYKDTALLRKFISDRGKIRS
                     RRVTGVTTQEQRQIARAVKVAREMALLPYAGAGRG"
     gene            2745..3191
                     /gene="rplI"
                     /locus_tag="VF362_03210"
     CDS             2745..3191
                     /gene="rplI"
                     /locus_tag="VF362_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010525625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX7589969.1"
                     /translation="MAKLILTHEVSGLGVAGDVVDVKDGFGRNYLLPRGLAAAWSAGA
                     EKQIEGLRRARRSKEISSVEDAHAARDALQSAPVIVKAKAGESGRLFGAVTPTIIADA
                     IGKRAVVDKRRIHVGQPIKALGEYQIQVGLHDNVSATVTLQVVAEA"
     gene            3641..4993
                     /gene="dnaB"
                     /locus_tag="VF362_03215"
     CDS             3641..4993
                     /gene="dnaB"
                     /locus_tag="VF362_03215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX7589970.1"
                     /translation="MSIQQLEASVGSFDRTPPQNIEAEQSVLGAMMLSKDAMADVVEV
                     VRAHDFYRPAHELVFDVAVALYNAGDPVDAITVSSRLQAAGTLGRIGGAEYLHTLISA
                     VPSAASAGYYARLVREQAILRRLVEAGTRIANMGYNADGDVDEIVDSAQSEVFAVTER
                     KNAEDYLPIGDVMERTLSEVEAASSHDGRLLGIPTGFRDLDELTGGFQGGQMIVIAAR
                     PAIGKSTLGLDICRAASIGAGATSVIFSLEMSREEITKRMLSAESGVKLTKMTKGPMG
                     PNDWERLAQTAARVSRAPLFIDDSPNMSLMEIRAKCRRLKQRENLGLVVVDYLQLMSS
                     GKKVESRQQEVAEFSRALKLLAKELDVPVIAISQLNRSPEQRGDKRPMLSDMRESGSI
                     EQDADIVILLHRDDAYDRDARPGEADFIIAKHRSGPTDTFAVAFKKDYAHFADMAPDL
                     "
BASE COUNT          854 a         1753 c         1663 g          780 t
ORIGIN      
        1 tacggcatcc aggccgccgc gcgcgagtac ttcaacgtca acgccgccga tctgacggtt
       61 tctcaggccg ccctcctcgc cggaatcatc ccggcgccga gcgcctggga cccccgcgtc
      121 gaccccgtcc aggccgaggc ccgatggaac tacgtgctcg acggaatggt caccgaggga
      181 tggttgacgc cgtccaaccg ccttcgcctc gacttcccca ccacggccga gtaccgcaag
      241 aacaacgtct tcggcggccc tcaggggtac ctcctgcgcg acgcgatcgt tgaggtcgcg
      301 gcgcggaccg gtctgtcgca ggacgccgtc gaaacggggg gctacacgat cgtcacctcg
      361 atcgactggg ccacgcagca ggcggcgcag aacgcggcgg cgacccttcc cgaggaccac
      421 agcccgaacc tgcgcctcgc catcgtcacg ctcgacccga ccacgggcgc catcacgtcc
      481 atgtacggcg gcgcggacta cgtgacgatc cagcgcgacg cggtgacgca ggacatcgct
      541 caagcgggct cgacgttcaa gccgttcgcg ctcatcgccg ccctggagaa cggcatcggc
      601 cttgagagcg tgtacagcgg cgcctccccc caggccatcg cggggttcac caagccggtg
      661 aagaacttcg ggaacgagaa cttcgggaac atcaccctca ccaccgcgac cgagcactcc
      721 gtcaacacgg tgtacgccca gctcgcccgc gacgtcggcc cggacaaggt cgtcgacgcc
      781 gccgtccggg cgggtcttcc cgcggatacc gccgggctcg aggcgaaccc cgcgaacgta
      841 cttggcacgg cctccccgca ccccctcgac atggcggcgg catatgcgac gatcgcggcg
      901 cagggggtgc gccacgaccc gttcctcgtc gggaccgtca aagacgccac gggcggggtg
      961 gtctacgcgc acgaactgaa agagaagcgc gagttcagcg ccgcggtgat ggccgacacg
     1021 acgttcgcgt tgcagcaggt ggtgctccac ggctcgggct cctacgccgc gaggctgagc
     1081 cgaccgatcg cgggcaagac cggaacctcg tcggacaaca agtccgcgtg gttcatcggg
     1141 ttcaccccgc agatcgtcgg agccgtcgcc atgtaccagg tgggtgcgga cggctcggtc
     1201 gaggcgatca ccccgttcgg ggggttcaag caaatcacgg ggggttcggt gccgtgccac
     1261 atctggaccg cgatgatggg cgaggtgctt cccaacctgc cggtcgagca attcccgccc
     1321 cgctcccacg tggggacggc gatcgtcccg agcccgagcc ccagcccctc cgtgaccccc
     1381 tcgccgacgc ccacgccctc ggcttccccg acgtcgggcg gcggggtcag cccgagcccg
     1441 agcctctcgc cgagtccgta gtttggggct gggcccgggc cgtttcccaa cgtcgtcccc
     1501 tccctgtacc cttgtgcggt ggttcccgcc cctgcgcggg gtcgccacag ctagcgcaac
     1561 cctcctgcca cggaaagccc gtggccgtcc agtccagagg aggtgggttc tttcatgcgc
     1621 aagtacgaaa tgatggtcat tctcgacccc gaggttgagg agcgcacggt tcagccgtcg
     1681 ctcgaccagt accttacggt cgtcaaagat gattccggca cggtcgacag gattgacatc
     1741 tggggccgtc gccgtctcgc gtacccgatc aagaagcgca cggacggcat ctacgccgtc
     1801 gtgcagttca cctcggaggc cgcgaccgcc aaggaacttg aacgtcaact gagtctcaac
     1861 gagaccgtgc tgcggaccaa gctcattcgg cccgacgcac actagtccag acgaaacgaa
     1921 cgaggacgaa ccatggcagg tgacacaacc atcacggtgg tcggcaactt gaccgccgac
     1981 ccggagttgc gtttcattca gtccggtgct gccgtcgtga acttcacggt ggcctccact
     2041 ccgcgtacgt ttgaccgcgc cacgaacgag tggaaggacg gcgagacgct gttcctgcga
     2101 tgctccctgt ggcgggaggc ggcggagaac gtcgccgaat cgcttaccaa gggcatgcgg
     2161 gtcatcgtct ccgggcgcct ggtgcaacgt tcgttcgagt ccagcgggga gaagcgcacg
     2221 gtcgtcgagc ttcaggtcga cgaggtgggt ccgtccctcc ggtacgcgac cgccaaggtc
     2281 acgcgcacgc agcgcgctgg cggcggtggc gctggcggcg gtggcggcgg aagcggattc
     2341 tcaggcgcgt cgtcgtcggc acccgcgggc ggctcggata gcgacccatg ggcgtccgct
     2401 cccccttcgg acgagcctcc gttctagacg ggctcggttc tagacggcct ccgtttcaga
     2461 catccttcat tcgcaactca tcatcatctt tcgggagtaa tcatggccaa gactgacctg
     2521 cggaagcccc gcaggaagac caacccgctg aaggccgcca aggtcgtgcg ggtcgactac
     2581 aaggacaccg cgctcctgcg gaagttcatc tccgaccgcg ggaagatccg ttcgcgccgc
     2641 gtcacgggcg tgacgacgca ggagcagcgg cagattgcgc gggccgtcaa ggtggcgcgc
     2701 gagatggcgc tgctgcccta cgcgggcgcg gggaggggct agcgatggcg aagctcattc
     2761 tcacgcatga ggtctccggc ctcggcgtcg ccggagacgt cgtcgacgtc aaggacggct
     2821 tcggtcgcaa ctaccttctt ccccgcgggc tcgccgcggc gtggtcggcc ggagcggaga
     2881 agcagatcga gggccttcgt cgcgctcgtc gctccaagga gatctcgagc gtcgaagatg
     2941 cccacgcggc ccgcgacgcc cttcagtccg cgcccgtgat tgtcaaggcg aaggcggggg
     3001 aatccggccg cctattcggc gccgtgacgc ccacgatcat cgcggacgcg atcggcaagc
     3061 gcgcggtcgt cgacaagcga cggatccacg tcggccagcc gatcaaggcg ctgggcgagt
     3121 accagatcca ggttggtctc cacgacaacg tgtccgcgac ggtgacgctt caggtcgtcg
     3181 ccgaggcgta ggtagccgtt tctcgaccgg agcccgggcc cctcgcaggg ttcgggcccc
     3241 cttcgtgaag gcgccaacga cggcgcgtcg cggcgccacg cccgacacgc ccgtgtccga
     3301 cacgccgggt ggttaccccc cggggttatc cccagcgcgc ccggggcatc gtgcctggta
     3361 gatgggcagg tttccgcttc taatcggagt tattcacatc tgtatccaca gtcacatacg
     3421 tcccttacgc cggttaccaa cagtccctcc acccctgggc ggccctgtcc acaggccggc
     3481 gaccgcggtc ggttgcgcgc ggcgccgggg cgggctaggt tcacccttca gacgccgtgc
     3541 gtcggggtga taggcaatgt cagtacctcg acgaatcatg gacaaggaag tccgtgggcg
     3601 ccgtccggcg cacggggggc gcgcagcgaa ggggagagcg gtgtcgattc agcagttgga
     3661 ggcgtcggtc gggagtttcg accgaacccc gccgcagaac atcgaggccg aacagtccgt
     3721 tctcggggcg atgatgctct cgaaggacgc gatggccgac gtcgtggagg tcgtccgcgc
     3781 gcatgacttc taccgtcccg cccacgaact ggtcttcgat gttgccgtcg ccctgtacaa
     3841 cgccggggac cccgtcgacg cgatcaccgt gtcgtcgcgg ctccaggccg cagggaccct
     3901 cggacgaatc gggggggccg agtaccttca caccctgatt tccgccgtcc cctcggcggc
     3961 gtcggcgggg tactacgcgc ggctcgtgcg cgagcaggca atcctccgcc ggctcgtgga
     4021 ggctggcacg cggatcgcga acatgggata caacgccgac ggcgacgtcg acgagatcgt
     4081 cgacagcgcc cagtccgagg tcttcgccgt caccgaacgc aagaacgccg aggactacct
     4141 gcccatcggc gacgtcatgg agcggaccct gagcgaggtc gaggccgcct cgagccacga
     4201 cgggcgcctc ctggggatcc cgacgggctt ccgtgacctc gacgagctca cgggcgggtt
     4261 ccagggcggc cagatgattg tcatcgcggc gcgccccgcg atcggcaagt ccacgctcgg
     4321 cctcgacatc tgccgcgccg cctccatagg cgctggcgcg acgtccgtca tcttctcgct
     4381 ggagatgagc cgcgaggaga tcactaagcg catgctctcc gcggagtccg gcgtcaaact
     4441 caccaagatg acgaagggcc ccatgggccc gaacgactgg gagcggctag cgcagacggc
     4501 ggcccgcgtg tcccgcgcgc ccctgttcat cgacgactcg ccgaacatgt cgctcatgga
     4561 gatccgcgcc aagtgccgcc gcctcaagca gcgcgagaac ctcggcctcg tcgtcgtcga
     4621 ctacctgcag ttgatgtcct ccggcaagaa ggtggagtcc cgccagcagg aggtcgcgga
     4681 gttctcccgc gcgctcaaac tgctcgcgaa ggaactcgac gtcccggtca tcgcgatctc
     4741 gcaactcaac cgttcgcccg agcagcgcgg cgacaagcgc cccatgcttt ccgacatgcg
     4801 agagtccggc tcgatcgagc aggacgcgga catcgtcatc ctcctgcacc gcgacgacgc
     4861 ctacgaccgc gacgcccgcc caggcgaggc ggacttcatc atcgccaagc accgctccgg
     4921 gccgacggac acgttcgccg tcgcgttcaa gaaggactac gcgcacttcg ccgacatggc
     4981 gccggatctg taggggctat tggggattca gttgtgggga cgaggtgcgc gaggtggcga
     5041 tgactgaaca
//
LOCUS       DASVKP010000155         3354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1531882, whole genome shotgun sequence.
ACCESSION   DASVKP010000155 DASVKP010000000
VERSION     DASVKP010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531882"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VF362_03220"
     CDS             <1..666
                     /locus_tag="VF362_03220"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEX7589971.1"
                     /translation="GSGNDAAYSYGLPLLDIEAAADRIYEGLQGDITSVDVGKVTGYG
                     VGEPGDPRYFTCVLSAGLDAAVAAYARTIVWPRGPIKYKFATFREVPRFHPYGVKVTA
                     DGKRWQGACTLVAVANSTVFGGGPIISPESKVNDGMLELVTAEDISRAGVLKLFPKLK
                     DGSHVKDPRITITRVKEVTITQTSDGARLPAAFADGELVGAVPLDIKIAPRALRILGA
                     RIP"
     gene            676..>3354
                     /locus_tag="VF362_03225"
     CDS             676..>3354
                     /locus_tag="VF362_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7589972.1"
                     /translation="MDDSPAERYRAARLRAAHPGVRAFAETLPFPLDDFQREGCEAIS
                     DGRSVLVAAPTGAGKTVVGEFAVRYARERGEKAFYTTPIKALSNQKFRELTAVHGPAA
                     VGLLTGDTSINGEADIVVMTTEVLRNMIYAGSRSLDRLGVVVLDEVHYLADRFRGSVW
                     EEVIVHLDQKTLIVALSATVSNAEEFGDWLTEVRGNTAVIVSETRPVPLWPHVLLREG
                     IYDLYSPGVDPAHPGPNPRLNPELEALARRQRQGDHKAHPTRMDYGRARGRPRPPARR
                     APPRFAVVDVLDERDLLPAIVFVFSRAGCDAAVDQVRSAGIALTTPEQRRQIADVIEA
                     RCAGMSGEDLGALGFTHWRDHLEAGIAAHHAGMIPLFKEIVEELFAAGLIKVVFATET
                     LALGINMPARCVVLEKLVKWDGDSHKDLTAGEYTQLTGRAGRRGIDIEGHAVVVDHPG
                     FDVEVLGRLASKRTYPLVSSFQPSYNMTLNLVVRSGVARAREVLEMSFAQYQADRSVV
                     AQARRVKELDGVLGGYRDAVACDRGDFIAYAALRERISQMQKSDVRRETRDRHEATAQ
                     GLRVLRVGAVLRILGGKRKGLVVVVAPDRSDQPRPVVVTSEARTFRLGPADLHQGFEE
                     VGEARVPKKFDARKAPARRELAAQLEARRAEFGPPPASRRRGPVRSGGSEADLDDARR
                     EMLAHPCHQCPDREDHARWAERYFRALRDRDRLAGEISLETGSIARIFDRRCAVLTEL
                     AYLSGSGDDLTPTEDGLMLRTVYSENDLAIAECLRSGAWDHLGAPGLAAAVSSLVYQG
                     RKEEDDRQPRIPQGPTGPLGLALRDTVRIWSRIDELHGEYRLPRAPTPHWGIVGAVHA
                     WTQGKNLDSVLRGTELAPGDMVRWFKQVIDVLDQVAH"
BASE COUNT          509 a         1168 c         1180 g          497 t
ORIGIN      
        1 ggctcgggca acgacgccgc ctacagttac ggtctcccgc tcctcgacat cgaggccgcg
       61 gcggaccgca tctacgaggg gctccagggt gacatcacct cggttgacgt cggcaaggtc
      121 acgggctacg gcgtgggcga gccgggcgat cctcgatact tcacgtgcgt cctttcggcg
      181 gggctcgacg ccgcggtggc cgcgtacgcc cgcaccatcg tctggccgcg gggcccgatc
      241 aagtacaagt tcgcgacgtt ccgcgaggtc ccccgattcc acccctacgg cgtcaaggtg
      301 acggcggacg gcaagcgatg gcagggggcg tgcacgctcg tcgcggtggc gaacagcacc
      361 gtcttcgggg gagggcccat catctcgccg gagtcgaagg tcaacgatgg catgctcgaa
      421 ctggtgaccg cggaggacat ctcccgcgcg ggcgtgctca aactcttccc gaagctcaag
      481 gacggctccc acgtgaagga tccgcgaatc accatcaccc gggtcaaaga agtaacgatc
      541 acccagacct cggacggcgc taggctcccc gccgccttcg cggacggcga actcgtgggg
      601 gctgttcccc tcgacatcaa gatcgcgcca cgggcgctgc gcatcctcgg agcccgcatc
      661 ccgtagcctc gatgcatgga cgattcgccc gcggagcgct accgggcggc gcggttgagg
      721 gcggcccatc cgggagttcg tgccttcgcc gagacccttc ccttccccct cgacgacttc
      781 cagcgcgagg ggtgcgaggc catttccgac ggccgctccg tgctcgtggc ggcgccgacg
      841 ggcgctggca agacggtggt gggcgagttc gccgtccgct acgcgcgcga gcggggcgag
      901 aaggcgttct acaccacgcc catcaaggcg ctctcgaacc agaagttccg cgagctcacc
      961 gcggtccacg gcccggcggc ggttgggctc ctgacggggg acacctcgat caacggcgaa
     1021 gccgacatcg tcgtcatgac gacggaggtg ctccgcaaca tgatctacgc gggctcgcgg
     1081 tcgctcgatc ggctcggcgt ggtggtcctc gacgaggttc actacctcgc ggaccgcttc
     1141 cgcggaagcg tgtgggagga ggtcatcgtc cacctggacc agaagaccct catcgtcgcg
     1201 ctctccgcga ccgtctcgaa cgccgaggag ttcggggact ggctcaccga ggtgaggggc
     1261 aacaccgccg tgatcgtcag cgagacgcgc cccgtccccc tgtggcctca cgtcctgctt
     1321 cgcgagggca tctacgacct ctactcgccg ggcgtcgacc ccgcgcatcc ggggccgaac
     1381 ccacgcctca acccggagct tgaggccctc gcgcgacggc agaggcaggg tgaccacaag
     1441 gcccatccca cccgcatgga ctacgggcgc gcgaggggcc gtccgcgccc gcccgcccgc
     1501 agggctcccc cccggttcgc ggtcgtcgac gtcctcgatg agcgcgacct gcttcccgcg
     1561 atcgtgttcg tgttctcccg cgcggggtgc gacgccgccg tcgaccaggt caggtcggcc
     1621 ggcatcgcgc tcaccacgcc ggagcagagg cggcagatcg ccgacgtcat cgaggcccga
     1681 tgcgcgggga tgagtggcga ggacctcggc gcgttgggct tcacgcactg gagggaccac
     1741 ctcgaggcgg ggatcgcggc gcaccacgcg gggatgatcc cgctgttcaa ggagatcgtc
     1801 gaggagttgt tcgcggcggg cctcatcaag gtcgtcttcg ccaccgagac cctcgcgctc
     1861 ggcatcaaca tgcccgcgcg ctgcgtcgtg ctggagaagc tcgtcaagtg ggacggcgac
     1921 agccacaagg acctgaccgc gggggagtac acgcaactca cgggtcgtgc aggcaggcgc
     1981 ggtatcgaca tcgaggggca cgcggtggtg gtcgaccacc cgggtttcga cgtcgaggtg
     2041 ctcgggcggc tcgcgtcgaa gcgcacgtac ccactcgtga gcagcttcca gccctcgtac
     2101 aacatgaccc tcaacctggt cgtgcgctcg ggcgttgcgc gcgcgcgcga ggtcctcgag
     2161 atgtccttcg cccagtacca ggcggaccgt tccgtcgtcg ctcaggcgcg gcgtgtgaag
     2221 gaactcgacg gggtgctcgg cgggtaccgc gatgccgtgg cgtgcgaccg aggggacttc
     2281 atcgcgtacg cagcgcttcg cgagcggatc tcccagatgc agaagtcgga cgtgcggcgg
     2341 gaaacccgcg accggcacga ggccacggcg cagggcctca gggtgctccg cgtgggcgcg
     2401 gtcctgagga ttctgggggg aaagcggaag ggcctcgtcg tggtcgtggc gcccgatcgc
     2461 agcgaccagc cgcgacccgt cgtcgtcacg tcggaggctc ggaccttccg gctcggaccc
     2521 gcggacctgc accaggggtt cgaggaggtc ggggaggctc gtgtcccgaa gaagttcgat
     2581 gcgcggaagg cccccgcccg cagggagctc gcggcgcaac tggaggccag gcgcgcggag
     2641 ttcggccctc cgcccgcgtc acgccgacgc ggtccggttc gctccggcgg ctccgaggcg
     2701 gatctggacg acgcgcggcg agagatgctc gcgcacccgt gccaccagtg cccggatcgc
     2761 gaggatcacg ctcggtgggc ggagcgctac ttccgtgccc tgcgagaccg cgatcgcctg
     2821 gcgggggaaa tctccctcga gacgggttcg atcgcccgca tcttcgaccg ccgctgcgcc
     2881 gtgctgacgg agctcgccta cctctccggc tcgggcgacg atctcacgcc caccgaggac
     2941 gggctgatgc tgcgcacggt ctactcggag aacgacctcg cgatcgccga gtgcctgcgc
     3001 agcggcgcgt gggatcacct cggcgccccc ggtctagcgg cggccgtctc gagcctcgtg
     3061 taccagggtc gcaaggagga ggatgatcgg cagccccgca tcccgcaggg gccgacgggt
     3121 ccgctcgggc tcgccctgcg cgacacggtc cgaatctggt ctcgcattga cgaactccac
     3181 ggggagtacc ggcttccgcg cgcgccgact ccgcattggg ggatcgtggg cgccgtccac
     3241 gcctggaccc aggggaagaa cctcgactcc gtgttgcggg gaacggagct cgccccgggc
     3301 gacatggtcc ggtggttcaa gcaggtgatc gacgtcctcg accaggtcgc gcac
//
LOCUS       DASVKP010000156         6193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_795339, whole genome shotgun sequence.
ACCESSION   DASVKP010000156 DASVKP010000000
VERSION     DASVKP010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795339"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VF362_03230"
     CDS             complement(<1..659)
                     /locus_tag="VF362_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589973.1"
                     /translation="MTKLSDLLRGAADRAPLGDASVSIGRAARRVRAQRGVRAAGNSV
                     AGLGAVALAVLGIVQPGSAMNSAQRDSAALAPGAAPVAGAQAPKDSMIASDGRGMAWG
                     TCGSFPLQDYGTTGTDALSIVPRFDTPAMPDGGSTLDIPVIVTANQAFDVTTNGPDAV
                     VLWQGMVVAVPSASQAEQSLTLAKGDTTQSVVSLPLVDCFTGAPLPASTYDLVVSQAF
                     M"
     gene            complement(688..1272)
                     /locus_tag="VF362_03235"
     CDS             complement(688..1272)
                     /locus_tag="VF362_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEX7589974.1"
                     /translation="MAEWHDAMEALMRDRERALLGYAYLVCGNATDAQDLVQDALVKT
                     FSRPRPGLTALKAEAYVRRAITTTYVDRYRRRQRWNAVQHLFRPGEGPADVMPELDME
                     ADIHAALALLPPRERACVVLRYFDDLTVPQIGRQLGIAGGSVKRYLADGIARLERSLG
                     SLEDTYSDSRGAYSSSADSRSAPSDFGASRLNRA"
     gene            1719..3557
                     /locus_tag="VF362_03240"
     CDS             1719..3557
                     /locus_tag="VF362_03240"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HEX7589975.1"
                     /translation="MTIAAVERAKPVINVEKSVQEYLDRSDWRVNANANQGYSLGGLI
                     LNTAGKVIANYWLSHVYAPQAGEAHRSGDLHIHDLDMFSGYCAGWSLRALLAEGFNGV
                     PGKVEASPAKHFSSAVGQIVNFLGTLQNEWAGAQAFSSFDTYMAPFIRFEGLDYKHVR
                     QGIQELIYNLNVPSRWGTQTPFTNLTFDWTCPADLKDQIPVIAGREMDFTYGDLQAEM
                     DMINRAYIDVMTTGDAAGRVFTFPIPTYNITKDFDWDSPNADLLFEMTAKYGLPYFQN
                     FINSDLEPHMVRSMCCRLQLDLRELLKRGNGLFGSAEQTGSVGVVTINCARLGYRFKG
                     DHDGLYTELDRLLDLARGTLEEKRKVIQEAIDDGLFPYTKRYLGTLRNHFSTIGINGV
                     NEMIRNYTGDAYDITTDEGEEMARELLDHVRETMVRFQEETTHLYNLEATPAEGTTYR
                     FAKEDARRFPGILQAGTTEQPYYTNSTQLPVGFTDDPFEALERQADLQTRYTGGTVLH
                     LYMRERVSSAEACKQLVKRALSSARLPYITVTPTFSICPFHGYLEGEQPTCPLCQLEF
                     PDREPQECEVWTRVMGYHRPVSSFNIGKKGEHAERVHFREESAILR"
     gene            3554..4333
                     /locus_tag="VF362_03245"
     CDS             3554..4333
                     /locus_tag="VF362_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEX7589976.1"
                     /translation="MTADDLAIAGFVPFSSIDWPTRLAATVFLQGCPWNCGYCQNPEL
                     IDPLLPGTLAWRTVRETLGRRVGLLDGVVFSGGEPLRQAALADAVAEVRDMGFEAAVH
                     TAGSYPARLEALLPSLAWVGLDIKAPPRLYGAVTGIEASGARAWASLDLVMGSGIDYE
                     VRVTVDPTVLGLSDVEEIVVELGRRGARMPVLQEVRSMGARPDYAARLGTTRLADVVP
                     EDAFPGIERRVEHVPIKGAPSIREVLASRDDGASDDQRMSA"
     gene            4471..>6193
                     /gene="topA"
                     /locus_tag="VF362_03250"
     CDS             4471..>6193
                     /gene="topA"
                     /locus_tag="VF362_03250"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX7589977.1"
                     /translation="MAGGRKLVIVESPTKSRTIGGLLGPDYEVVSSIGHIRDLPQPSE
                     MPEGKREGSLGKFAVDVENGFEPYYVISPEKKKVVAEMRASLKNATEVYLATDEDREG
                     EAIAWHLLEVLKPKVPVKRLAFHEITPEGIRRALEHPREVDMELVEAQEARRVLDRLY
                     GYEVSPVLWRKVASGLSAGRVQSIALRLVVDRERERIAFRAAEFWDVRATFAATMGPD
                     VGRTFDARLTSVDGKRVATGRDFDDKGTLKADGVVHVTAALAGALASGLASAEFSVVS
                     VESKPYTRKPAAPFITSSLIQEASRKFRLGARESMRVAQGLYERGYITYMRTDSPILS
                     GEALRAARAQAAELYGAETVSEAPRVYASSDANAQEAHEAIRPAGDRFKTPASIQGEL
                     RGDEFRLYDLIWKRTVASQMKDAKGTTSTVRVGAQSAAHHAVEFSASGTIITFPGFLA
                     AYEESHEASRYEEEEAQALKVDGDGARLPRLEEGQAVRSDSLDPTVHRTTPPPRFTQG
                     SMIKELEERKFGRPSTYAATVDLIVARQYVRIDRQALIPTWIAFSIVGLMEKHFDWLI
                     DYDFTADM"
BASE COUNT         1024 a         2167 c         2068 g          934 t
ORIGIN      
        1 ttcatgaagg cctggctgac gacgaggtcg taggtgctcg cgggaagtgg ggcgcccgtg
       61 aagcagtcga cgaggggcag ggagacgacg gactgcgtcg tgtctccctt ggcgagggtg
      121 agcgactgct ctgcctgact cgcgctcggg accgccacca ccatcccctg ccacaggacg
      181 acggcgtcgg ggccgttcgt cgtgacgtcg aaagcctggt tcgcagtgac gatcacgggg
      241 atgtcgagcg tcgagccccc gtccggcatg gccggcgtgt cgaaccgcgg cacaatggaa
      301 agcgcgtcgg ttccggtggt tccgtagtcc tgcagcggga acgagccgca cgtgccccag
      361 gccatcccgc gtccgtcgga cgcgatcatg gagtccttcg gcgcctgggc gcccgcgacg
      421 ggcgcggccc ccggggcgag cgcggcggag tcccgctgcg ccgagttcat cgccgaaccg
      481 ggctgcacga tgccgagaac cgcgagcgcc acggcgccga ggccggcgac gctattgccc
      541 gcggcgcgga cgccgcgctg cgcccgcacc ctgcgggccg cccggccgat ggaaacggac
      601 gcgtcgccca gcggcgcgcg atcggcggcg cccctgagga ggtcggagag cttggtcatg
      661 tcctgtcctt tctatacgtc tgtgcggtca cgcgcggttg aggcgcgacg cgccgaagtc
      721 ggaaggtgcg gagcgggagt cggcgctgct ggaataagca ccgcgggagt cggagtaggt
      781 gtcctcgagg gagccaaggc tgcgctcgag tcgggcgatg ccgtcggcga ggtagcgctt
      841 gacgctgccg cccgcgattc ccaattggcg cccgatctgc ggcaccgtga ggtcatcgaa
      901 gtagcggagt accacgcacg cacgctcgcg gggaggaagg agggcgaggg ccgcgtgaat
      961 gtcggcctcc atgtcgagct ctggcatcac gtcagcgggg ccttcgcccg gccggaagag
     1021 atgctggacc gcgttccatc tttggcggcg gcggtaccgg tcaacgtagg tggtcgtgat
     1081 ggctcggcgc acgtatgcct cggccttgag ggcggtgagg ccggggcgcg gtcgcgagaa
     1141 ggtcttgacg agcgcgtcct gcacgaggtc ctgagcatcg gtcgcgttgc cgcagaccag
     1201 gtaggcgtat ccgaggagcg cccgctcgcg gtcgcgcatc agggcctcca tcgcgtcatg
     1261 ccactcggcc atatctcctc cgtcgtggcc ctggggcccc tcttgcaggt gttgacgaac
     1321 gcgcggcggg aatcgttggg tcggcgccgc ctcttcccct cacccattgt tctccccgac
     1381 ccccccgacc cacggggcgg cgcctcgaac tccccgcccc ggtcgtcaag gaggacgcat
     1441 cgtcgcgagc gtcgcggcgc gcgtcggcga ggctcccggg ggtgtcagac cccggacgta
     1501 gggtgggggt tcacgtcgac gattggcgtg tagcgaaggg tttccgggcc cggggaggcc
     1561 atccgcgccg aacccgggac taaagtccta ccccgcgttc ccggatgcgg agtagacaca
     1621 acatatgggt gcagaacccg agtgagccac tagatgttgt ggtcctagga acaccccttc
     1681 aagcgtcaat accaagccat gaaagcaagg gagccgacat gaccatcgcc gccgtcgagc
     1741 gagccaagcc cgttatcaac gtcgagaagt ccgtccagga atacctggac cgcagtgact
     1801 ggcgcgtcaa cgcgaacgcc aatcagggct actccctcgg cggcctgatc ctcaacaccg
     1861 cgggcaaggt gatcgccaac tactggctga gccacgtcta cgcgccccag gcaggcgagg
     1921 cgcaccgcag cggtgacctc cacattcacg acctcgacat gttctcgggc tactgcgcgg
     1981 gctggtccct gcgcgcgctc ctcgccgagg gattcaacgg cgtccccggc aaggtcgagg
     2041 cgagcccggc gaagcacttc tcttccgccg tcgggcagat cgtcaacttc ctcggcacgc
     2101 tccagaacga gtgggcgggc gcgcaggcgt tcagctcgtt cgacacgtac atggcgccgt
     2161 tcatccgatt cgagggcctc gactacaagc acgtgcgcca gggaatccag gaactcatct
     2221 acaacctcaa cgtcccctcg cgctggggca cccagacgcc gttcaccaac ctcaccttcg
     2281 actggacctg ccctgcggac ctcaaggacc agatcccggt gatcgcgggg cgcgagatgg
     2341 acttcaccta cggcgacctc caggccgaga tggacatgat caaccgggcc tacatcgacg
     2401 tcatgacgac gggcgacgcg gccgggcggg tcttcacgtt cccgatcccc acgtacaaca
     2461 tcaccaagga cttcgactgg gacagcccca acgccgacct cctgttcgag atgaccgcca
     2521 agtacggact cccgtacttc cagaacttca tcaactccga cctcgagccc cacatggtgc
     2581 gctcgatgtg ttgccgcctt cagctggacc tgcgcgaact cctcaagcgc ggcaacgggc
     2641 tcttcggctc ggccgagcag acgggttccg tcggcgtcgt caccatcaac tgcgcgcgcc
     2701 tcggctaccg cttcaagggc gaccacgacg gcctctacac ggagctcgac cgccttctcg
     2761 acctcgctcg cggcacgctc gaggagaagc gcaaggtcat ccaggaggcg atcgacgacg
     2821 gcctcttccc gtacaccaag cgctacctcg gcacgctccg caaccacttc tcgaccatcg
     2881 ggatcaacgg cgtcaacgag atgatccgca actacacggg cgacgcgtac gacatcacca
     2941 ccgacgaggg cgaggagatg gcgcgcgagc tcctcgatca cgtccgcgag acgatggtgc
     3001 gcttccagga ggagaccacc cacctctaca acctcgaggc gacccccgca gagggcacaa
     3061 cgtaccgctt cgccaaggag gacgcccgtc gtttcccggg catcctccag gccggaacca
     3121 ccgagcagcc gtactacacc aactccacgc aacttcccgt ggggttcacg gacgacccgt
     3181 tcgaggcgct cgagcgccag gcggacctcc agacgcggta cacgggcggc accgtcctcc
     3241 acctctacat gcgggagcgc gtgtcgagtg cagaggcctg caagcaactc gtcaagcgcg
     3301 cgctctcctc ggcgcggttg ccgtacatca cggtgacgcc cacgttctcc atctgcccgt
     3361 tccacggtta cctcgagggc gagcagccca cgtgcccgct gtgccagctc gaattccccg
     3421 acagggagcc gcaggagtgc gaggtctgga ccagggtcat gggctaccac cgccccgtct
     3481 cgagtttcaa catcggcaag aagggcgagc acgcggagcg cgtccacttc cgtgaggaat
     3541 cagcgatcct gagatgacgg ccgacgacct cgcgatcgcg gggttcgtgc cgttctcgtc
     3601 gatcgattgg ccgacgaggc tcgccgctac ggtcttcctc cagggttgcc cgtggaactg
     3661 cgggtactgc cagaaccccg agctgatcga cccgctcctt ccggggaccc tcgcctggag
     3721 gaccgttcgc gagacgcttg ggcgtcgcgt cgggctcctg gatggcgtgg tgttctcggg
     3781 gggagagccc ctccggcagg ccgcgctcgc cgacgccgtc gccgaggttc gagacatggg
     3841 atttgaagcg gccgtccaca ccgcgggttc ctaccccgcg aggcttgagg cactcctccc
     3901 ttcgctcgcc tgggtgggcc tcgacatcaa ggctccgccg cgcctgtacg gcgcggtcac
     3961 ggggatcgag gcgagcggcg cgcgcgcgtg ggcctccctc gacctggtca tggggtccgg
     4021 gatcgactac gaggttcgcg tgaccgtcga ccccacggtc ctcggactgt ccgacgtcga
     4081 ggagatcgtc gtggaactcg gcaggcgagg cgcgcggatg cccgtgctcc aggaggtccg
     4141 ctccatgggt gcacgtccgg actacgcggc caggctcggc accacccggc tcgccgacgt
     4201 cgtccccgag gacgcgttcc ccggcatcga gcgaagggtc gagcacgtcc cgatcaaggg
     4261 cgcgccgagc atccgtgagg tgctcgcgag ccgggatgac ggggcctcgg acgaccaacg
     4321 gatgtccgca tagcgttcgc tcggcccacc cgcccccagc acgcgtcgct gcttcccgtc
     4381 gcgaagctcg agttgctaca cctacagtgg tggcgggtat cctgcccgca aggcaccctg
     4441 cacgggcgcc acgtcacgga aggcgaacat atggccggcg gccgcaagct tgtcatcgtc
     4501 gagtcgccga cgaagtcgcg cacgatcggc gggcttctgg gcccggatta cgaggttgtg
     4561 tcgtcgatcg gccacatccg cgacctccct cagccgtccg agatgcccga gggcaagcgc
     4621 gagggttccc tcggcaagtt cgcggtggat gtggagaacg gcttcgagcc gtactacgtg
     4681 atctcgccgg agaagaagaa ggtcgtcgcc gagatgcgag cgagcctgaa gaacgcgacc
     4741 gaggtctacc tcgcgaccga tgaggaccgc gagggcgagg ccatcgcctg gcacctgctc
     4801 gaggtcctga agccgaaggt tccggtcaag cgcctggcgt tccacgagat cacccccgag
     4861 ggaatccggc gcgcgctcga gcacccccgc gaggtggaca tggaactggt cgaggcgcag
     4921 gaggccaggc gcgtcctcga ccgcctctac ggatacgagg tatcgcccgt cctgtggcgc
     4981 aaggtcgcgt cgggactcag cgcggggcgc gtccagtcca tcgccctccg cctcgtggtg
     5041 gatcgggagc gcgagcgcat cgcgttccgc gccgccgagt tctgggacgt gcgtgccacc
     5101 ttcgcggcga cgatgggccc cgacgttggc cgaaccttcg acgcgcgcct gacgtccgtg
     5161 gacggcaagc gcgtcgcgac cggccgcgac ttcgacgaca agggcacgct caaggcggac
     5221 ggcgtcgtcc acgtcaccgc cgcgctcgcc ggggccctcg cctccggcct cgccagtgcg
     5281 gagttctccg tggtgagcgt cgagtccaag ccgtacacgc ggaagcccgc cgcccccttc
     5341 attacctcga gcctcatcca ggaggcgagc cgcaagttcc gcctcggggc gcgcgagtcg
     5401 atgcgcgtcg cccagggcct ctatgagcgc ggctacatca cctatatgcg tacggactcg
     5461 ccgatcctgt ccggcgaggc gctacgcgcg gcgcgggccc aggcggccga gctctacggg
     5521 gccgagaccg tctccgaggc gccgcgcgtc tacgcgtcgt ccgacgccaa cgcgcaggag
     5581 gcccacgagg cgatccgtcc cgcaggcgac cggttcaaga cgccggcgag catccaggga
     5641 gagctgcggg gcgacgagtt ccggctgtac gacctcatct ggaagcgcac cgtcgcgagc
     5701 cagatgaagg acgccaaggg caccacctcc acggtgcgcg tgggcgccca gagcgcggct
     5761 caccacgccg tcgagttcag cgcctccggc accatcatca ccttccccgg cttcctggcc
     5821 gcctacgagg agtcgcacga ggcctcgcgc tacgaggagg aggaggcgca ggcgctcaag
     5881 gtcgacggag acggcgcccg ccttccgcgc ctcgaggagg gtcaggccgt gcgctcggac
     5941 tcgctcgatc cgaccgtgca ccgcacgacg ccgccgccgc gattcaccca gggctcgatg
     6001 atcaaggagc ttgaggagcg gaagttcggc cgcccctcca cctacgccgc gacggtggac
     6061 ctcatcgtcg cgcgccagta cgtgcgcatc gaccgccagg cgctcatccc cacgtggatc
     6121 gcgttctcca tcgtcgggct catggagaag cacttcgact ggctcatcga ctacgacttc
     6181 accgccgaca tgg
//
LOCUS       DASVKP010000157         3470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1291540, whole genome shotgun sequence.
ACCESSION   DASVKP010000157 DASVKP010000000
VERSION     DASVKP010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291540"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VF362_03255"
     CDS             <1..196
                     /locus_tag="VF362_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589978.1"
                     /translation="APKMEAALRAVKGGVPQVHIIDGRVPHAILTEIFTTEGIGTMIL
                     AGDPPKKKAAAKSEQGEVHS"
     gene            193..1422
                     /locus_tag="VF362_03260"
     CDS             193..1422
                     /locus_tag="VF362_03260"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002874.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEX7589979.1"
                     /translation="MTDGATPTGAGEGGAILARYGEALMGTYGAPTRALVKGEGNYLW
                     DADGRKYLDLLGGIAVTAIGYAHPAWVKAVADQAATLAHVSNFFATGPQVALAERLLD
                     LAHAPTGSRVFLCSTGTEANEAALKAARKTGRHVVVALEGGFHGRTVGALAVTHKPAI
                     REPFGPFGGEVRFVVPGDVEALERAMTGEVAAVILETIQGEAGVRPLTDEFLAAARRL
                     CDEHGALLIIDEVQTGIGRTGTWFSHQAHGIAPDILTLAKGLGGGFPIGAVVAYGERA
                     ATLLTRGDHGSTFGGNPLACAAALATLDVIEDEGLIENAKAVGEHIRAGALALAGVAE
                     VRGDGLMLGIVLDTPAASEVVAAAIEAGFIINAPQADTIRLVPALTLSAAEADTFLAW
                     LGEHLAAGEAGTKETDS"
     gene            1419..2348
                     /gene="argF"
                     /locus_tag="VF362_03265"
     CDS             1419..2348
                     /gene="argF"
                     /locus_tag="VF362_03265"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX7589980.1"
                     /translation="MTRHFLRDDDLTPSEQREVLELALAFKRDRYYSQPFAGPQAVAV
                     LFDKHSTRTRVSFSVGIAELGGFPLVLDAGTSQMGRGESITDTARVLERQVAAIVWRT
                     HGQERIVQMAQASGVPVVNALTDEFHPCQILADLAAVADGRGGVDALKGLTLAYLGDG
                     ANNMAHSYLLGGATAGMHVRIGTPEAYRPDPAVVARAAEIAATTGGSVAVVHTHDEAA
                     TGADVIATDTWISMGDEAEAEARATAFLPFQVSSATLALAAPGAQVLHCLPAYRGKEI
                     TADVLDGPQSIVWDEAEYRLHAQKALLTWLVDQ"
     gene            2345..2908
                     /gene="argR"
                     /locus_tag="VF362_03270"
     CDS             2345..2908
                     /gene="argR"
                     /locus_tag="VF362_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018779117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEX7589981.1"
                     /translation="MTVPPNAGVPATKAARQALIRRVLEATPVRSQGQLAIELEARGL
                     AVTQATLSRDLVDVGAVKVRGADGNLLYAAGDSVAASEDTENRLNRLIAELLFSADGS
                     ANLAVLRTPSGAAQFLASAIDSHGDPDIVGTVAGDDTVLVVARETDGGLRLAQKFLDR
                     VHSDRAHSGSAYSGRAHSTATQKEHLS"
     gene            2905..3435
                     /locus_tag="VF362_03275"
                     /pseudo
     CDS             2905..3435
                     /locus_tag="VF362_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012398617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
BASE COUNT          540 a         1263 c         1207 g          460 t
ORIGIN      
        1 ggcgcccaag atggaggctg cgctccgcgc ggtcaagggc ggcgtgcctc aggtccacat
       61 catcgacggg cgcgtccccc acgcgatcct cacggaaatc ttcaccacgg agggaatcgg
      121 cacgatgatc ctcgcgggcg atccgcccaa gaagaaggcg gcggcgaaat cggaacaagg
      181 cgaggtgcac tcatgacgga cggcgcgacg cccacgggcg caggcgaggg cggtgcgatc
      241 ctcgcgcggt acggcgaggc gctgatgggc acctacggcg cgcccacgcg cgcgctcgtc
      301 aagggcgagg gcaactacct ctgggacgcc gacggtcgga agtacctcga cctcctcggc
      361 ggaatcgcgg tcaccgcgat cgggtacgcc caccccgcgt gggtgaaggc cgttgcggac
      421 caggccgcga cgctcgcgca cgtgtccaac ttcttcgcga cgggccccca ggtcgcgctc
      481 gcggagcgcc tgctcgacct tgcgcatgcc ccgacgggct cccgcgtctt cctgtgcagc
      541 accggcaccg aggcgaacga ggcggcgctc aaggcggcgc gcaagaccgg ccggcacgtc
      601 gtcgtcgccc tcgagggcgg cttccacgga aggacggtcg gcgcgctcgc ggtgacccac
      661 aagcccgcga tccgggagcc cttcggcccg ttcggaggcg aggtccgctt cgtcgtcccc
      721 ggcgacgtcg aggcgcttga gcgcgcgatg acgggcgagg tcgccgccgt catcctggag
      781 acgatccagg gcgaggcggg cgtccggccc ctgaccgacg aattcctcgc ggccgcgagg
      841 cggctatgcg acgagcacgg agccctgctc atcatcgacg aggtccagac gggaatcggc
      901 cgcaccggca cgtggttctc gcaccaggcg cacggcatcg cgccggacat cctgacgctc
      961 gccaagggac tcggaggggg tttccccatc ggcgccgtcg tcgcctacgg cgagcgggcg
     1021 gccacgctgc tcacgcgggg agaccacggc tcgacgttcg gaggtaaccc cctcgcgtgc
     1081 gcggcggccc tcgcgaccct cgatgtcatc gaagacgagg ggctgatcga gaacgccaag
     1141 gcggtggggg agcacatcag ggcgggcgcc ctcgcgctcg ccggggtcgc ggaggtccgc
     1201 ggcgacgggc tgatgctcgg catcgttctc gatacccccg cggcttccga ggtggttgcc
     1261 gccgcgatcg aggcgggctt catcatcaac gcgccccagg cggacaccat caggctcgtt
     1321 cccgcgctca cgctgagcgc ggcggaggcg gacacgttcc tcgcgtggct cggggagcac
     1381 ctcgccgcgg gcgaggcagg gacgaaggag acggactcat gacgcggcac ttcctcaggg
     1441 acgacgacct caccccgtcc gagcagcggg aggtgcttga actcgcgctc gcgttcaagc
     1501 gcgaccgcta ctactcccag cccttcgcgg gccctcaggc ggttgcggtg ctgttcgaca
     1561 agcactcgac gcgcacgcgc gtctccttct ccgtcggcat cgccgagctg ggagggttcc
     1621 ccctcgtcct cgatgccggg acctcccaga tgggcagggg ggagagcatc accgacaccg
     1681 cccgcgtgct cgagcgccag gtcgccgcca tcgtttggcg gacccacggc caggagcgga
     1741 tcgttcagat ggcgcaggcc tccggcgtgc ccgtcgtcaa cgcgctcacc gacgagtttc
     1801 acccgtgcca gatcctcgcc gaccttgcgg ccgtcgcgga cgggcgcggg ggagtggacg
     1861 ccctcaaggg gctgacgctc gcctacctcg gcgacggggc gaacaacatg gcccactcct
     1921 acctgctcgg cggagccacc gcgggcatgc acgtgcggat cgggacgccc gaggcgtacc
     1981 ggcccgaccc cgccgtcgtg gcgagggcgg ccgagatcgc cgcgacgacc ggggggagcg
     2041 tcgccgtcgt tcacacccac gacgaggccg cgacgggggc ggacgtgatc gccaccgaca
     2101 cgtggatctc catgggggac gaggcggagg ccgaggcccg cgccaccgcc ttcctgccgt
     2161 tccaggtcag ctcggcgacg ctcgcccttg ctgcaccggg ggctcaggtg ctccactgcc
     2221 tgcccgcgta ccgcggcaag gagatcaccg cggacgtgct cgacggcccc cagagcatcg
     2281 tgtgggacga ggcggagtat cgccttcacg cccagaaggc gctgctgacg tggctggtcg
     2341 atcagtgacg gttcccccga acgctggcgt cccggcgacc aaggccgcgc ggcaggcgct
     2401 cattcgccgc gtcctcgagg ccacgcccgt gagaagccag ggccaactcg cgatcgaact
     2461 cgaggcgcgg ggcctggccg tcacccaggc gacgctctcg cgcgacctcg tcgacgtcgg
     2521 cgccgtgaag gtgcgggggg ccgacgggaa cctcctctac gcggcgggag actcggtcgc
     2581 cgccagcgag gacaccgaga accgactcaa ccgcctgatc gcggagctcc tcttcagcgc
     2641 cgacgggtcg gcgaacctcg ccgtcctccg cacgccctcc ggggccgccc agttcctcgc
     2701 gagcgccatc gactcccacg gcgaccccga catcgtaggt acggtcgccg gggacgatac
     2761 cgttctggtc gtcgcccgcg agactgacgg cgggcttcgg ctcgcccaga agttcctcga
     2821 ccgcgttcac tcagaccgag cccattcagg cagtgcctac tccggcaggg cccattccac
     2881 cgccacacag aaagagcacc tctcatgaag cagcgcatcg tcctcgccta ctccggcgga
     2941 ctcgacacct ccgtcggcat cggctggatc gaggacgcca cgggcgccga ggtcatcgcc
     3001 gtggccgtcg acgtcggcca gggcggggag gacatggacg tcatccgcca gcgcgcgctc
     3061 gattgcggcg ccgtcgaggc gtacgtcgcc gacgctcggg acgagttcgc gacggaatac
     3121 tgcatgccgg cgctcgccgc gaacgcgctc tacatggacc gctacccgct cgtctcgtcg
     3181 ctcagccgcc cggtgatcgt gaagcacctc gtcaccgccg cgaagcagtt cggcgcgacg
     3241 acggtcgccc acgggtgcac gggcaagggc aacgaccagg tgcggttcga ggtgggcatc
     3301 gtctcgctcg cgccggagct gacctgcatc gcgcccatcc gcgacctcgc cctgacgcgc
     3361 gagaaggcga tcgactacgc cgaggcgcac aacctcccca tagcgacgac caagaagaac
     3421 cccttcagca tcgactagaa cgtctgggga cgcgccgtgg agacgggctt
//
LOCUS       DASVKP010000158         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_217632, whole genome shotgun sequence.
ACCESSION   DASVKP010000158 DASVKP010000000
VERSION     DASVKP010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217632"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VF362_03280"
     CDS             <1..1145
                     /locus_tag="VF362_03280"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018134832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX7589982.1"
                     /translation="DVDLHYYDLSIENRDATDDQVTIDAAHAIREHGVGVKCATITPD
                     EARVEEFGLKEMWKSPNGTIRNILGGVVFREPIVISNIPRLVPGWTKPIVIGRHAHGD
                     QYKATDFKVPGAGSVTLTYTPADGSAPVEMEVVNMPEGGGVVMGMYNFNESIADFARA
                     SLRYGLDRGFPVYLSTKNTVLKAYDGAFKDIFADVFEREFKADYDKAGLTYEHRLIDD
                     MVAAAMKWEGGYVWACKNYDGDVQSDTIAQGFGSLGLMTSILVTPDGKTVEAEAAHGT
                     VTRHYREHQKGNPTSTNPIASIFAWTGGLKHRGKLDGTPEVTAFAATLEDVVIATVEE
                     GHMTKDLALLIGPNQAYQTTEDFLATVDANLVASLNGASAKGGALA"
     gene            1142..2134
                     /locus_tag="VF362_03285"
     CDS             1142..2134
                     /locus_tag="VF362_03285"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX7589983.1"
                     /translation="MSAPSPIRLTLTGAAGQIGYALLFRIASGQMFGPDVRVALRLLE
                     VTPALKVAEGAAMELADCAFPLLASVDIFDDATAAFDSANIGLLVGARPRGPGMERAD
                     LLEANAGIFAPQGAAINAGAAEDVRILVVGNPANTNALIASAHAPDVPPTRFTAMTRL
                     DHNRAVAQLALKTGASTRDIRNVIIWGNHSATQYADVAHATIGGAPALQVVGDETWVR
                     DVFIPTVARRGTAILETRGSSSAASAANAIIDHVRDWTAGTRDGEWTSVALPSNGSYG
                     VNEGLFSSYPAVSKDGAWEIVQGLEMDEYARGKIAESVAEMIEERDAVRALGLI"
BASE COUNT          386 a          767 c          749 g          333 t
ORIGIN      
        1 tcgacgttga cctgcactac tacgacctct cgatcgagaa ccgcgatgct accgacgacc
       61 aggtgacgat cgacgccgcc cacgcgatca gggagcacgg agtcggcgtc aagtgcgcga
      121 cgatcacccc ggacgaggct cgcgtcgagg agtttggcct caaggagatg tggaagtccc
      181 ccaacggaac catccgcaac atcctcgggg gcgtcgtctt ccgcgagccc atcgtcatct
      241 cgaacatccc gcgcctcgtg cccggctgga ccaagccgat cgtgatcggg cgtcatgccc
      301 acggcgacca gtacaaggcg acggacttca aggtccccgg cgccggaagc gtgaccctga
      361 cgtacacccc ggccgacggc tccgcgccgg tggagatgga ggtggtgaat atgccggagg
      421 gtggcggcgt tgtcatgggc atgtacaact tcaacgaatc cattgccgac ttcgcccgcg
      481 cctcgctccg ctacgggctt gaccgcggct tcccggtcta cctctccacg aagaacaccg
      541 tcctcaaggc ctacgacggc gcattcaagg acatcttcgc cgacgtcttc gagcgcgagt
      601 tcaaggcgga ctacgacaag gccgggctga cctacgagca ccggctgatc gacgacatgg
      661 tggcggccgc catgaagtgg gagggcggct atgtctgggc atgcaagaac tacgacggcg
      721 acgtccagtc ggacaccatc gcccagggct tcggttcgct cgggctgatg acgtcgattc
      781 tggtgacgcc cgacggcaag acggtcgagg cggaggccgc gcacggcacg gtgacgcggc
      841 actaccgcga gcaccagaag ggcaacccca cctccaccaa cccgatcgcg tcgatcttcg
      901 cctggaccgg gggcctcaag catcgcggca agctcgacgg cacgccggag gtcacggcct
      961 tcgccgcgac gctcgaagac gtggtgatcg ccaccgtgga agagggacat atgacgaagg
     1021 acctcgcgct cctcattggg ccgaaccagg cgtaccagac gaccgaggac ttcctcgcga
     1081 cggtcgacgc caacctcgtg gcgtctctca atggcgcgtc tgccaagggc ggggcgctgg
     1141 cgtgagcgcg ccgagcccta tccggcttac cctgacgggg gcggctggcc agatcggcta
     1201 cgcgctcctg ttccgcattg cctccggaca gatgttcggt cccgacgtgc gcgtggcgct
     1261 tcgactcctt gaggtgactc ccgcgctcaa ggtggccgag ggtgcggcga tggaactcgc
     1321 ggactgcgcg tttcccctgc tcgcgagcgt cgacatcttc gacgatgcca ccgcggcctt
     1381 cgacagcgcg aacatcggct tgctcgtcgg cgctcgccca cgcggccctg gcatggagcg
     1441 cgcggacctc ctcgaggcga acgcgggcat cttcgccccg cagggcgccg cgatcaacgc
     1501 cggcgccgcg gaggacgtgc gcatactcgt cgtcgggaac cccgcgaaca cgaacgcgct
     1561 gatcgcctcc gcgcacgcgc cggacgtccc cccgactcgc ttcaccgcga tgacgcgact
     1621 ggaccacaac cgggcggtcg cgcagctcgc gctcaagacc ggcgcctcca cccgcgacat
     1681 ccggaacgtg atcatttggg gcaaccactc ggccacgcag tacgcggacg tcgcccacgc
     1741 gacgatcggc ggggcgccag cgctccaggt ggtcggggac gagacctggg tgcgcgacgt
     1801 cttcatcccg acggttgcga ggcgcgggac ggcgatcctc gagacccggg gttcgtcctc
     1861 cgctgcgtcc gcggccaacg cgatcatcga ccacgttcgc gactggacgg cggggacgcg
     1921 tgacggcgaa tggacctcgg tggccctccc gtcgaacggc tcctacggcg tcaacgaggg
     1981 gctcttctcg tcctatcccg cggtctcgaa ggacggggcg tgggagatcg tccagggact
     2041 cgagatggac gaatacgcga ggggcaagat cgccgagtcg gtcgcggaga tgatcgagga
     2101 gcgggacgcg gttcgcgcgc tcggcctgat ttagcccgtc agcccgaatc ccagcgggaa
     2161 gagatactcg cctcccggcg ggacggaggg caactggccg atgtcgcggg gccagttcat
     2221 cgggagcgtg cccgt
//
LOCUS       DASVKP010000159         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1532246, whole genome shotgun sequence.
ACCESSION   DASVKP010000159 DASVKP010000000
VERSION     DASVKP010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532246"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VF362_03290"
     CDS             complement(<1..120)
                     /locus_tag="VF362_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX7589984.1"
                     /translation="MTIFADPLMQRALIVALLVGLSSPVIGTYLVQRRMALLGD"
     gene            complement(123..896)
                     /locus_tag="VF362_03295"
     CDS             complement(123..896)
                     /locus_tag="VF362_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7589985.1"
                     /translation="MTSPALHAADVRVAIGGFPVLHGISFDIAAGETVALMGGNGSGK
                     TTLVRAIIGAPPLTSGSIEIDGRATSPDARAHLGYVPQRVSASAGVSATATEIVVSGL
                     LRGRRLAPPPRARARALEALQAVGLADLAGRDVNAMSGGQQQRVLIARAMVRRPTLLL
                     LDEPVAGVDLPSQEVFANALESLKAGGATIVVVLHEVGMLADLIDRAVVLDRGRVVHI
                     GEPPHAHGDHAVPGHDHIHPHDDDPNRPGPETPTFEIAV"
     gene            complement(889..>1624)
                     /locus_tag="VF362_03300"
     CDS             complement(889..>1624)
                     /locus_tag="VF362_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX7589986.1"
                     /translation="ITALAPPGVEPHDLELSPAAVRSVRNSDVVLYLSGFQASVDDAV
                     KTTGAPALDVADSVALLPAPAGLALSFDPHFWLDPTLLSVYAGAVGKEFATLDPAHAE
                     EYRANATKLENDLATLDANYQAGLAACERHTILTSHDAFGYLAARYGLTQVGLSGLDP
                     EAEPSPAKIREARALAVSSGATTVFIEALVDPSVVQAFASDAHLTVATLDPIESLVPS
                     TLVANPSYITAMEQNLSALRTALACD"
BASE COUNT          229 a          536 c          635 g          224 t
ORIGIN      
        1 gtcgccgagg agcgccatcc tccgctgcac caggtaggtg ccgatcacgg gggaggagag
       61 tcccacgagg agcgcgacga tgagcgcgcg ctgcatgagg gggtcggcga agatggtcat
      121 cgtcacaccg cgatctcgaa cgtcggggtt tccggccccg gccggttcgg gtcgtcgtcg
      181 tgcgggtgaa tgtggtcgtg gcccggcacg gcgtggtcgc cgtgcgcgtg gggcggctcg
      241 ccgatgtgga cgacgcggcc gcgatcgagc acgacggccc gatcgatgag gtccgcgagc
      301 atccccacct cgtggaggac gaccacgatc gtcgcccccc ccgccttgag gctctcgagc
      361 gcgttcgcga agacctcctg gctcgggagg tcgacgcccg cgacgggctc gtcgaggagg
      421 aggagcgtgg ggcgtcgaac catcgcgcgc gcgatcagga cgcgctgctg ctggccgccg
      481 ctcatcgcgt tgacgtcccg accggcgagg tccgcaagcc cgaccgcctg gagcgcctcg
      541 agcgcccgcg ccctggcgcg gggcggggga gccaggcggc ggccccggag gagcccggag
      601 acgacgatct ccgtcgccgt cgcgctcacc ccggcggacg cgctgacccg ctgcgggacg
      661 tagccgaggt gcgcccgcgc gtcgggcgac gtggcccgcc cgtcgatctc gatcgacccg
      721 gatgtcaggg ggggcgcgcc gatgatcgcc cgaacgagcg tcgtcttccc cgacccgttg
      781 ccgcccatca gggcgacggt ctctcccgcg gcgatgtcga acgagatccc gtggagcacg
      841 ggaaagcccc cgatcgccac gcggacgtcg gcggcatgga gcgccggact agtcacaggc
      901 gagcgccgtc ctgagcgcgg ataggttctg ttccatggcc gtaatgtaac tgggattcgc
      961 gacgagggtc gacggcacga ggctctcgat cgggtcgagg gtcgcgaccg tgaggtgagc
     1021 gtcggaggcg aacgcctgga ccaccgacgg gtcgacgagt gcctcgatga agaccgtcgt
     1081 cgcgccgctc gacaccgcga gcgccctcgc ctcgcgtatc ttcgcggggc tcggctcggc
     1141 ctcggggtcg aggccggaca gcccgacctg ggtgaggccg tatcgggcgg cgaggtaccc
     1201 gaaggcgtcg tggctggtga ggatcgtgtg gcgctcgcac gccgcaaggc ccgcctggta
     1261 gttggcgtcc aacgtcgcga ggtcgttctc gagcttcgtt gcgttcgcgc ggtactcctc
     1321 ggcgtgcgcc gggtcgaggg tggcgaactc cttgccgacc gcacccgcgt agacggagag
     1381 cagagtcggg tccagccaga agtgcgggtc gaacgagagg gcgaggcccg ccggtgcggg
     1441 gaggagggcg accgagtcgg cgacgtcgag cgcgggggcg cccgtcgtct tgaccgcgtc
     1501 atcgaccgac gcctggaagc cggagaggta gaggacgacg tccgagttcc ggacggaccg
     1561 tacggcggcc ggggagagtt cgaggtcgtg cggttccacc ccggggggtg cgagcgccgt
     1621 gatc
//
LOCUS       DASVKP010000160         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_382955, whole genome shotgun sequence.
ACCESSION   DASVKP010000160 DASVKP010000000
VERSION     DASVKP010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382955"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1494)
                     /locus_tag="VF362_03305"
     CDS             complement(214..1494)
                     /locus_tag="VF362_03305"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX7589987.1"
                     /translation="MAYAEEFEKALQVGRPPNIQALFPNSKALIVSGKVVDRAMRAKG
                     KAIAMAANGRNSFVIRGALQAAQRADAALIIEIAKSEGGQKAYCAVNYWNMARIVDAL
                     CNEMGITIPVAVHADHYGMKNDKDLAQAMVEVPTMFEAGITSIAIDASHQPNDQNLLA
                     NLAVNPKIPAWAGLETEIGEIKGSTGLSTPDEALFLIQGLNAHGIFADWIALNNGTTH
                     GIEASDAGIQVALTTEVHEALTPYNVSGAQHGTSGNSSERLREIAAKTHTTKANVATA
                     LQMISWGLEVNDYGNAQLDADGDFIKVKGEGVTEEMWAEVVAYADDKGWKKGDYKNLN
                     LPFESKFLAQPAEIRERMARRVEDFVYKMLTEVLGTADTAPLAVEAILAAGSHDLGAK
                     VGRLEDPADWTDAKIVEKAKSLDSDKGAKGEFDD"
BASE COUNT          189 a          506 c          528 g          300 t
ORIGIN      
        1 ctgtcgcccg agcaggcgat ggcggagctc cgcgccggcg ccggctccca gttcgatccg
       61 cagctcgtgg aggcgtttat cgaggggctg cagaaggcgg acggggcggg cggctgacgc
      121 cgcccgcccc gtccgtgttc actcactcgc gcctcgcgtc acgcagtgtg gggtgcccct
      181 gcggggcccc ccacgttgac ccgcctgcgg tggtcaatcg tcgaactccc ccttggcgcc
      241 cttgtcgctg tcgagcgact tcgccttctc gacgatcttc gcgtcggtcc agtcggcggg
      301 gtcctcgagc cgtccgacct tggcgccaag gtcatgggac ccggcggcga ggatcgcctc
      361 gacggcgagg ggagcggtgt ccgccgtgcc caggacctcg gtgagcatct tgtagacgaa
      421 gtcctcgacc cgccgggcca tccgctcgcg gatctccgcc ggctgggcga ggaacttgct
      481 ctcgaacgga aggttgaggt tcttgtagtc gcccttcttc cagcccttgt cgtcggcgta
      541 ggcgaccacc tcggcccaca tctcctcggt cacgccctcg cccttcacct tgatgaagtc
      601 gccgtcggcg tcgagctgcg cgttgccgta gtcgttgacc tcgagccccc aggagatcat
      661 ctggagggcg gtggcaacgt tggccttggt cgtgtgggtc ttggccgcga tctcgcggag
      721 acgctcggag ctgttgcccg acgtgccgtg ctgggcgccg ctgacgttgt agggcgtgag
      781 ggcctcgtgg acctcggtgg tcagcgcgac ctggatgccg gcgtcggatg cctcgatccc
      841 gtgggtggtc ccgttgttca gcgcgatcca gtcggcgaag atgccgtgcg cgttgagccc
      901 ctggatgagg aacagcgcct cgtccggcgt cgagagcccg gtggagccct tgatctcgcc
      961 gatctccgtc tccagcccgg cccacgccgg gatcttcgga ttgacggcga ggttggccag
     1021 caggttctgg tcgttgggct ggtgggacgc gtcgatggcg atggacgtga tgcccgcctc
     1081 gaacatcgtg gggacctcga ccatggcctg cgcgaggtcc ttgtcgttct tcataccgta
     1141 gtggtcggcg tggacggcga cggggatcgt gatccccatc tcgttgcaca gggcgtcgac
     1201 gatgcgcgcc atgttccagt agttcaccgc gcagtaggcc ttctgccctc cctcggactt
     1261 ggcgatctcg atgatgaggg cagcgtcggc ccgctgggcc gcctgcagcg ccccgcggat
     1321 aacgaaggag ttccgcccgt tcgcggccat cgcgatggcc ttgcccttcg cccgcatcgc
     1381 ccggtcgacg accttgccgc tgacgatcag cgccttcgag ttcgggaaga gtgcttggat
     1441 gttgggagga cggcccacct ggagggcctt ctcgaactcc tctgcgtacg ccatggaacg
     1501 tctccttcgg ggggttgcgg atc
//
LOCUS       DASVKP010000161         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_796278, whole genome shotgun sequence.
ACCESSION   DASVKP010000161 DASVKP010000000
VERSION     DASVKP010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796278"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="VF362_03310"
     CDS             <1..794
                     /locus_tag="VF362_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7589988.1"
                     /translation="STTAARTPARLAARRFAPALLAAAFWIAVWQVVAMVVHRDFLLA
                     SPGAVVVRLGELVVTGGFWSTVATSLLRIIVGFTGAAIVGAVLATLSAASRVAEALAA
                     PLIATIRSAPVVSFIILLLLWTDSSRLAAYTSFLMVLPVMYANILAGIRNRDRALLEV
                     ATVFRIPWLRRVRAIDVPAVLPFFAAACRTGVGLAWKSGVAAEVIGIAQGSIGERLYE
                     AKLFLESADLFAWTIVIIALSVTGEAVVLWGLRRAQARFATGGAS"
     gene            791..1390
                     /locus_tag="VF362_03315"
     CDS             791..1390
                     /locus_tag="VF362_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7589989.1"
                     /translation="MSTVRTGGIALRGVTRRFGEQTVLDGLDLTLPAGTTTALMGPNA
                     SGKTTAARLLLGLDAPDLGVVDGLDGLRCAAVFQEHRLCGHLSAVENLRLVLPHADWH
                     RVDDELSRVGLVGEAQTKPVRELSGGQRRRVAIARAMIVDADFIVLDEPFTGLDVDVK
                     PLVLSYVKERVFGRTALLITHDRAEARALGATIARLPGR"
     gene            1503..>2255
                     /locus_tag="VF362_03320"
     CDS             1503..>2255
                     /locus_tag="VF362_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7589990.1"
                     /translation="MVTTPPSQNSPWPLALRATYGLAAVVLAGGLVTLLVPNLLTGPA
                     VMNGSAKGTALAMIAVGMPIAVVAALRTRRTGSPVAHAVWLGASAYLVYNSVALCFGT
                     PFNGAFLLYTTGLGLAILVTALLVRAYAPASFGGWTSRRLERAIGVFLVVVTTLNALV
                     WLKVVVPALGAPSDATFLEGSGLITNPFYVQDLAWWIPAFIAAGIGLWRGHPFARLIA
                     GAAVVDFSIESATIAIDQFWGHQSDPASTVATL"
BASE COUNT          291 a          805 c          764 g          395 t
ORIGIN      
        1 cttctactac ggcggctaga acgccggcac ggctagcagc gcggagattc gctcccgcgt
       61 tgctagctgc agcgttctgg atcgccgtct ggcaggtagt cgcgatggtc gtccatcgcg
      121 atttcttgct ggcatccccc ggcgcagtcg tggttcgcct gggcgagctc gtcgttaccg
      181 gcgggttctg gtcgacggtc gcaacttcgc tactgcggat catcgtgggc ttcaccggcg
      241 ccgcgatcgt gggcgcggtt ctcgcgacgc tctcggcggc gtcgcgcgtc gccgaggccc
      301 tcgcggcacc actgatcgcg acgatccgaa gcgccccagt cgtgagcttc atcatcctct
      361 tgctgctgtg gaccgactcg agccgtctcg cggcctacac gagcttcctc atggtcctgc
      421 ccgtcatgta cgcgaacatc ctcgcgggga tccgcaatcg cgaccgcgcg ctcctcgagg
      481 tcgcgaccgt cttccgcatc ccgtggctac gccgcgtccg ggcgatcgac gtccccgccg
      541 tgcttccctt cttcgccgcg gcgtgccgca ccggcgtcgg ccttgcctgg aagagcgggg
      601 tggccgccga ggtgatcggc atcgcccagg gaagcatcgg tgagcgactg tacgaggcga
      661 agctcttcct cgagtcggcc gacctcttcg cctggacgat cgtcatcatc gcgctgagcg
      721 tcacgggcga ggccgtcgtg ctgtgggggc ttcggcgggc gcaggcgcgg tttgctacgg
      781 ggggtgcgtc gtgagcaccg tccggacggg gggaatcgcc cttcgggggg tcacccggcg
      841 gttcggcgaa cagacggtct tggacggcct cgacctgacg ctcccggcgg ggacgacgac
      901 ggcgctcatg ggacccaacg cctcggggaa gacgacggcg gcacggcttc tcctcgggct
      961 cgacgccccg gatctcggcg tggtagacgg gctcgacggg ctgcgatgcg ccgcggtgtt
     1021 ccaggagcac cgcctgtgcg ggcacctcag cgcggtcgag aaccttcggc tcgttctgcc
     1081 ccacgcggac tggcaccgcg tcgacgacga actctcacgc gtcggactag tcggcgaggc
     1141 acaaaccaag cccgtgcgcg agctctccgg gggccaacgc cgccgcgtcg cgatcgcgcg
     1201 cgcgatgatc gtggacgccg atttcatcgt gctcgatgag cccttcaccg ggctcgacgt
     1261 cgacgtcaag cccctcgtcc tctcctacgt gaaggagcgc gtgttcggcc gcaccgccct
     1321 gctcatcacc cacgaccgcg cggaagctcg tgccctcggc gccacgatcg ctcgcctgcc
     1381 gggacgctag ttcccccagc gaccgcgtcg gggacctagg gccctcgacc ggcgcaccgg
     1441 attcctcgac catggtgagg agcggccgca gcgccgcgcc agggcacgag gagaggcaca
     1501 ccatggtcac gacaccgcct tcacagaaca gcccctggcc cctggccctg cgagcgacgt
     1561 acggcctagc cgccgtcgtc ctggcgggcg gactcgtgac gctcctggtt ccgaacctgc
     1621 tgaccggccc cgcggtcatg aacgggtcgg ccaagggtac ggcgctcgcg atgatcgccg
     1681 tcggtatgcc catcgccgtg gtggccgcgc tacgcacgcg gcgaacgggc tcccccgttg
     1741 cccacgcggt ctggctcggc gcctcggcct acctggtcta caactcggtc gcgctgtgct
     1801 tcggcacccc gttcaacggg gcgtttctcc tctacacgac ggggctcggc ctcgcgatcc
     1861 tcgtgacggc tctgctcgta cgggcgtacg cgcccgcctc gttcgggggc tggacgagca
     1921 ggcgcctcga acgcgcgatc ggcgtgttcc tcgtggtcgt cacgacgctc aacgcgctcg
     1981 tgtggctcaa ggtggtggtg cccgcgctcg gcgctccgtc cgacgccacg ttcctcgagg
     2041 ggtccgggct cattaccaac ccgttctacg tccaggacct tgcctggtgg attccggcct
     2101 tcatcgcggc gggaatcggc ctgtggcgcg ggcatccgtt cgcaaggctc attgcgggag
     2161 cggcggtggt cgatttctcg atcgaatccg cgacgatcgc gattgaccag ttctgggggc
     2221 accagtcgga ccccgcctcg acggtggcga ccttg
//
LOCUS       DASVKP010000162         6986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1209529, whole genome shotgun sequence.
ACCESSION   DASVKP010000162 DASVKP010000000
VERSION     DASVKP010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209529"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="VF362_03325"
     CDS             complement(<1..107)
                     /locus_tag="VF362_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinic acid mononucleotide
                     adenylyltransferase"
                     /protein_id="HEX7589991.1"
                     /translation="MTVDTKAARIGVLGGTFDPIHIGHLAAASEVFAALS"
     gene            complement(215..1531)
                     /locus_tag="VF362_03330"
     CDS             complement(215..1531)
                     /locus_tag="VF362_03330"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX7589992.1"
                     /translation="MTDTTISAEVSSTEVSSAEVRDAVLDACTRAKVASRVLATANRS
                     VKDAALHAMADALVAQAGLILEANARDVERERADGMAPGLLDRLTLDEGRVVAMAAAL
                     RALAALPDPVGEVRRGSTLPNGLRLIQKAVPMGVIGMIYEARPNVTVDAAGLALKSGN
                     AAVLRGGSAAAESNEAIVGVLRSALGAVGLPADAVQSIDAYGREGARVLMNARGLVDV
                     LVPRGGRELIQAVVTGSTVPAIETGEGNCHVYLDASAPLERSIAITLNSKTHRVGVCN
                     AAETLLVHRDAAPRVLPAVLAALAEAGVTLHADAASAALAPAGVATVPATEEDWATEY
                     LSYDMAIRVVESLDEAITHIQRYSTGHTEAIVTDDLASAEAFVARLDTAAIMVNASTR
                     FTDGGEFGLGAELGISTQKLHARGPMGLAELTTTTWVVYGEGQIRP"
     gene            complement(1542..2669)
                     /gene="proB"
                     /locus_tag="VF362_03335"
     CDS             complement(1542..2669)
                     /gene="proB"
                     /locus_tag="VF362_03335"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX7589993.1"
                     /translation="MEDVRAALTAPGRVVVKVGSSSLTGADGHLDEVALVALVDVLAG
                     MHSRGQQVVLVTSGSIAAGLVPLGLSARPTDLATAQATASVGQGLLIGAYARAFARHA
                     LNVGQVLLTADDLTRRAHYGNAQRALDRLLELGVVPIVNENDTVATDEIRFGDNDRLA
                     ALVATVVHADALVLLTDVDGLYTAAPGTPGARRIASVAAFEEIEGVDISQRGHSLGTG
                     GMVTKLEAARIATGSGVAVVLAAARDAAAAVAGEDVGTIFAPTGKRSTSRLQWLAHAA
                     KTRGQLVLDEGAVRAVRGGKASLLAAGVSAVRGEFEAGDPVELVGPDGRAIARGFARF
                     AAHQIPAMLGLSTAAIRSTLGEEYARELVHVDDLVVVATGK"
     gene            complement(2669..4204)
                     /gene="obgE"
                     /locus_tag="VF362_03340"
     CDS             complement(2669..4204)
                     /gene="obgE"
                     /locus_tag="VF362_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX7589994.1"
                     /translation="MFVDRVTLHVSGGKGGNGVASVHREKFKPLGGPDGGNGGNGGSV
                     ILVVDSQMTTLLDYHYSPHKMARNGAPGAGDMRDGANGADIRLAIPNGTTVKTLDGAI
                     LADMVGEGAEFVVAAGGRGGLGNAGLATRKRIAPGFALLGEPGDDAEVVLELKSIADV
                     ALVGYPSAGKSSIIAAMSAVRPKIADYPFTTLTPNLGVVEAGGSRFTMADVPGLIPGA
                     SEGKGLGLEFLRHIERCSVIAHVLDAAAMESDRDPLRDLDVISQELAAYAADLAGGQL
                     PLLERPQVVILNKIDIPDGRELASIVRESIEARGLPVYEVSAVTHEGLPALGYALAAL
                     VAAARAVGPAPEPARIVLRPKAVDDVGFTVTRRVLDDEVFFQVRGVKPERWVRQTDFT
                     NEEAVGYLADRLATLGVEDELFRAGAVPGAEVVIGPVEGGVIFDWEPTLMAGAELLGG
                     RGTDIRVAEHEHLPRESRSEKRAKHLGRMDAKAAAREELGTERDAGHWVSPDDDGGRE
                     GEK"
     gene            complement(4346..4597)
                     /gene="rpmA"
                     /locus_tag="VF362_03345"
     CDS             complement(4346..4597)
                     /gene="rpmA"
                     /locus_tag="VF362_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX7589995.1"
                     /translation="MAHKKGASASRNGRDSSPQYLGVKRFGGQVVKAGEIIVRQRGTH
                     FHPGLNVGRGKDDTLFALEHGAVKFGTRRGRRVIDIVAS"
     gene            complement(4609..4917)
                     /gene="rplU"
                     /locus_tag="VF362_03350"
     CDS             complement(4609..4917)
                     /gene="rplU"
                     /locus_tag="VF362_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX7589996.1"
                     /translation="MVYAIVKAGGRQEKVSVGDIVVVDRLAADEGATVELPALLLVDG
                     STITSDAKALAKIKVTAQVVRDERGPKIHILKYKNKTGYRKRQGHRQDLTRLTITGIG
                     "
     gene            complement(5045..>6986)
                     /locus_tag="VF362_03355"
     CDS             complement(5045..>6986)
                     /locus_tag="VF362_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX7589997.1"
                     /translation="KKAPAKRAPAKKPAAAAASEGDAPGDYVKDHADSSGHDGAGRDS
                     DGGDGDESGEGGTRRRRRRGGRGRGTRARSGGGGDEGEARETRDSETPDDADGGSDSS
                     RRRRNRGGRTRGAGASDDLAGSTRLQAKRQRRREGRDTRRPAITEAEFLARRESVKRS
                     MVVREKDGRVQIAVLEDDILVEHYVSHQAQQSMAGNVYLARVQNVLPGMEAAFVDVGR
                     GRNAVLYAGEVNWDAAGMEGKPRRIELALKPGDTIMVQVTKDPIGHKGARVTSQISLA
                     GRFLVYVPGGGVTGISRRLPDNERTRLKKLLREVIPPDAGVIVRTAAEGASEDELRAD
                     VERLKRQWEGIEKEAATGKAPQLLRGEPDMAIRVVRDIFNGDFDSLTIQGEDTWNSLG
                     AYIAQVAPDLQERIHRYVGSGDVFHDARVDEQLQKGMARKVWLPSGGSLVIDRTEAMT
                     VIDVNTGKFIGKGGTLEETMTLNNLEAAEEIVRQLRLRDIGGIIVVDFVDMLLEANRD
                     RVLRRLIECLGRDRTKHQVAEVTSLGLVQMTRKRVGQGLVEAFSETCEVCKGRGFLVH
                     GEPVIETAERTPAPARRKGRGNAEVEEPVETHALDDHSEAREAVKATLASIAAAAEKA
                     HYHHDDHDHDVPAADGDDDEAE"
BASE COUNT          958 a         2470 c         2460 g         1098 t
ORIGIN      
        1 gaaagcgcgg cgaatacctc gctggctgct gcgagatgcc cgatgtggat gggatcgaac
       61 gttccgccca gaaccccgat gcgagccgct ttggtgtcca cggtcagtgg cgcgcaccga
      121 cgctccgcaa cgcgaaggcg acgaagagac gggtcacaac tgctccttca cgcgtagacc
      181 ttcattgtcg cacgcgtccc gccagccgcg cccgtcacgg gcggatctgg ccttcgccat
      241 acacgaccca cgtcgtcgtc gtcagctccg cgaggcccat ggggccgcgc gcgtgaagct
      301 tctgcgtcga gatccccaac tccgcaccga gcccgaactc gccgccgtcg gtgaagcgcg
      361 tcgacgcgtt gaccatgatc gccgcggtgt cgaggcgggc aacgaacgcc tcggcggagg
      421 cgaggtcgtc cgtgacaatc gcctcggtgt ggccggtgga gtagcgctgg atgtgcgtga
      481 tcgcctcgtc gaggctttcc accaccctga tggccatgtc gtaggacagg tactccgtcg
      541 cccagtcttc ctcggtggct ggcacggtcg cgaccccggc aggggcgagg gcggccgatg
      601 ccgcgtccgc gtggagcgtg accccggcct ccgcgagcgc ggccaggact gcgggaagga
      661 cccgcggcgc ggcgtcccga tgcaccagga gggtctcggc cgcgttgcag acgccgacgc
      721 gatgggtctt ggagttcagg gtgatggcga tcgagcgctc gaggggcgcg gacgcgtcga
      781 ggtagacgtg gcagttgccc tcgccggttt cgatcgccgg caccgtcgat cccgtcacca
      841 ccgcctggat cagctcgcgg ccgccccggg gcaccagcac gtcgacgagg ccgcgtgcgt
      901 tcatcagcac gcgggccccc tctcgcccat aggcatcgat gctctggacg gcgtcggcgg
      961 gcaacccgac ggcgccgagc gcggagcgca ggacgccgac gatggcctcg ttggactcgg
     1021 ccgcggccga tcccccgcgc agcaccgcgg cgtttcccga cttgagcgcg agcccggcgg
     1081 catccaccgt gacgttcggc cgcgcctcgt agatcatgcc gatgacgccc atcgggacgg
     1141 ccttctggat gaggcggagc ccattgggaa gagtcgagcc gcggcgaacc tcgccgacgg
     1201 gatcgggaag cgccgcgaga gcacgcaggg cggccgccat cgcgacgacc cgcccctcgt
     1261 caagcgtcag ccgatcgagg agccccggcg ccatcccgtc ggcgcgttcc cgctccacgt
     1321 cgcgcgcgtt cgcctcgagg atgagtcccg cctgggcgac cagtgcgtcc gccatggcgt
     1381 gaagcgccgc gtccttgacg gagcggttgg ccgtggcgag gacgcgggag gcgaccttgg
     1441 cgcgcgtgca cgcatcgagc acggcgtcgc gcacctctgc gcttgaaacc tcggtgctgg
     1501 aaacctcggc gctgatggtg gtgtcggtca tgcggccaag gctacttgcc cgtcgcaacg
     1561 acgacgaggt cgtcgacgtg gacgagctcc ctcgcgtact cctcgccgag cgtcgagcgg
     1621 atggcggccg tgctgaggcc gagcatcgcg ggaatctggt gtgcggcgaa gcgcgcgaac
     1681 ccccgggcga tcgccctccc gtctggcccc acgagttcga cggggtcccc tgcctcgaac
     1741 tcgccgcgga ccgcgcttac ccccgcggcg aggagcgacg ccttgccccc cctcaccgcc
     1801 ctgaccgcgc cctcatcgag cacgagttga ccgcgcgtct ttgcggcgtg cgcgagccac
     1861 tgcagccgac tcgtcgagcg cttgcccgtc ggggcgaaga tggtgccgac gtcctcgcca
     1921 gctaccgctg ccgcggcgtc ccgcgcagcc gcgagcacca ccgcgacgcc ggagcccgtc
     1981 gcgatgcgcg ccgcctctag cttggtcacc atgccgccgg tcccgagcga gtggccccgc
     2041 tgggagatgt cgacgccctc gatctcctcg aatgccgcga cgctcgcaat gcgccgagct
     2101 cccggggtgc cgggagccgc cgtgtagagg ccgtccacgt ccgtgagcag gacgagggca
     2161 tccgcgtgga ccacggtcgc gacgagggcc gcgaggcggt cgttgtcgcc gaatcggatc
     2221 tcgtcggtcg cgacggtgtc gttctcgttc acgatcggca ccacgcccaa ctccaggagg
     2281 cgatcgagcg cccgttgggc gttgccatag tgggcgcgac gcgtgaggtc gtcggcggtg
     2341 aggagcacct ggccgacgtt gagggcgtgc cgggcgaacg cgcgggcgta ggcgccgatg
     2401 aggagcccct ggccgacgga cgccgtcgcc tgtgccgtcg cgaggtccgt ggggcgcgcg
     2461 ctgaggccga ggggcacgag ccccgccgcg atggaaccag acgtcacgag gacgacctgc
     2521 tgccctcggg agtgcattcc cgcgaggacg tcgacgagcg cgacgagcgc gacctcgtcg
     2581 aggtggccat cagccccggt aagcgacgac gaaccgacct tcaccaccac gcgaccgggc
     2641 gccgtcagcg ccgcccgcac gtcctccatc acttctctcc ttcgcgcccg ccgtcatcat
     2701 ccggcgacac ccagtgtccc gcgtcgcgct cggtcccgag ctcctcacgc gccgcggcct
     2761 tcgcatccat gcggccgagg tgtttggccc tcttctccga acggctctcc cgcgggaggt
     2821 gctcatgttc cgccacgcgg atgtccgtgc cgcgaccgcc cagtagttcg gcgcccgcca
     2881 tgagcgtcgg ctcccagtcg aagatgacgc cgccctcgac ggggccgatc acgacctccg
     2941 cacccggcac ggcgcccgcg cggaagagct cgtcctcaac gccgagcgtc gcgaggcggt
     3001 cggcgaggta gccaaccgcc tcctcgttcg tgaagtccgt ctggcgcacc cagcgctcgg
     3061 gcttgacgcc gcgaacctgg aagaacacct cgtcgtccaa gacgcggcgc gtcacggtga
     3121 accccacgtc atcgacggcc ttgggccgca gcacgattcg cgcgggttcg ggcgccggcc
     3181 cgaccgctcg cgccgccgcg acgagcgcgg cgagggcata gccgagcgcg ggcaggccct
     3241 cgtgggtcac ggcgctgacc tcgtagacgg gaagcccgcg ggcctcgatc gactctcgca
     3301 cgatcgaggc gagttcgcgc ccgtcgggga tgtcgatctt gttgaggatg acgacctgcg
     3361 ggcgctccag gaggggcagt tgaccgcccg caaggtccgc ggcatacgcc gcgagttcct
     3421 gcgagatgac gtcgagatcc ctgagggggt cgcgatcgct ctccatcgcg gcggcatcga
     3481 ggacgtgcgc gatcacgctg caccgctcga tgtggcgcag gaattcgagc ccgagcccct
     3541 tgccctccga ggcgcccggg atcagcccgg ggacgtcggc catggtgaat cgcgacccgc
     3601 ccgcctcgac gacgccgagg ttgggggtga gggtggtgaa ggggtagtcg gcgatcttgg
     3661 ggcgcacggc ggacatcgcg gcgatgatag atgacttgcc cgcgctcggg tagccgacga
     3721 gggcaacgtc cgcgatcgac ttgagctcga ggacgacctc cgcatcatcc cccggctcgc
     3781 cgaggagggc gaagcccggc gcgatgcgct tgcgcgtcgc gaggcccgcg ttgccgagcc
     3841 cgccccggcc ccccgcggcg acgacgaact ccgcgccctc gcccaccatg tccgcgagaa
     3901 tggcgccgtc gagcgtcttg accgtggtgc cgttgggtat cgcgagccgg atgtccgcgc
     3961 cgttcgcgcc gtcgcgcatg tcgccagcgc cgggcgcgcc gttccgagcc atcttgtgcg
     4021 gagagtagtg atagtcgagg agcgtcgtca tctgggagtc gacgacgagg atcaccgagc
     4081 ccccgttgcc gccgttgccg ccgtcgggcc cgccgagggg cttgaacttc tctcggtgga
     4141 cggatgcgac gccgttgccg cccttgccgc ccgagacgtg gagcgtgacg cgatcgacga
     4201 acacgtctct cccctttcgc ttccgtctgt catcaacgat acggccggtt gccgccgacg
     4261 acggatcggc cgacgcacga cggctgggtg atcgtgtgac tcgcgccggg tggcgcgagc
     4321 gggcccgcgg ggccccggag cgacgctagg aggcgacgat gtcgatgacg cgtcgcccgc
     4381 ggcgcgtgcc gaacttcacg gcgccgtgct cgagcgcgaa cagggtgtca tccttgccgc
     4441 gacccacgtt caggccgggg tggaagtgcg tgccgcgctg gcggacgatg atctcgcccg
     4501 ccttgacgac ctggccgccg aagcgcttga cgccgaggta ctgcgggctg gagtcgcggc
     4561 cgttccgtga ggcgcttgcg cccttcttgt gtgccatcgc gtatcccctt agccgatgcc
     4621 cgtgatcgtg aggcgcgtga ggtcctgacg gtgtccctgg cgcttccgat atccggtctt
     4681 gttcttgtac ttgagaatgt gaatcttggg gccgcgctcg tcgcgaacga cctgggcggt
     4741 caccttgatc ttggcgagcg ccttggcgtc gctcgtgatc gtggagccat cgaccagcag
     4801 gagtgccggc aactcgaccg tggcgccctc gtctgccgcc aaacggtcga cgacgacgat
     4861 gtcgccaacg gagacctttt cctgacggcc tccggccttg acaatcgcgt acaccatggc
     4921 attcctcaag tctcggtgga ttcccgcgcg gccggctccc gaaggacggc gcgcagggtt
     4981 gggcgcaaga gcgccgacgg acaacgatac aaggccgccg ggccccggtc aaacctgcgg
     5041 ggacttactc ggcctcgtca tcgtccccat ctgccgcggg aacatcgtga tcgtggtcgt
     5101 cgtggtggta gtgagccttc tccgcggcgg ccgcgatgct cgcgagcgtc gccttgacgg
     5161 cctcgcgcgc ctcgctgtga tcgtcgagcg cgtgcgtctc gacgggctcc tccacctcgg
     5221 cgttgccgcg acccttgcgc cgcgcgggcg cgggtgtgcg ctccgcggtt tcgatgacgg
     5281 gctcgccgtg gacgaggaat ccgcgaccct tgcacacctc gcacgtctcg gagaaggcct
     5341 cgaccaggcc ctgcccgacg cgcttgcgcg tcatctgcac gaggccaagg ctcgtcacct
     5401 cggcgacctg atgcttggtg cggtcgcgcc ccaggcactc gatgaggcgg cgcagcacgc
     5461 ggtcgcggtt ggcctccagc agcatgtcga cgaagtcgac gacgatgatt ccgccgatgt
     5521 cacggagcct caactggcgg acgatctcct ctgcggcctc gaggttgttg agggtcatcg
     5581 tctcttcgag cgtgccgccc ttgccgatga acttgccggt gttgacgtcg atgaccgtca
     5641 tcgcctcggt acggtcgatg acaagcgatc cgccggaggg cagccagacc ttcctggcca
     5701 tgcccttctg gagctgctcg tcgacgcgcg cgtcgtggaa gacgtcgccg gatccaacgt
     5761 agcggtggat ccgctcctgg agatcgggcg cgacctgggc gatgtaggcg cccagcgagt
     5821 tccacgtgtc ttcgccctgg atggtcaggg agtcgaagtc gccgttgaag atgtcgcgaa
     5881 cgacgcggat ggccatgtcg ggctcgccgc gcaggagttg cggcgccttg cccgtcgccg
     5941 cctccttctc gatgccctcc cactggcgct tcaggcgctc gacgtccgcg cgaagctcgt
     6001 cctccgaggc tccctcggcg gccgtgcgaa cgatgacgcc cgcgtccggc gggatgacct
     6061 cgcggagcaa cttcttgagg cgggtgcgct cgttgtccgg gaggcgtcgg gagatgcccg
     6121 tgactccgcc gccgggcacg tagacaagga agcgaccggc gagcgaaatc tgcgaggtga
     6181 cgcgcgcgcc cttgtggccg atggggtcct tggtgacctg gaccatgatg gtgtcgcccg
     6241 gcttgagcgc gagctcgatg cggcggggtt tgccctccat gcccgcggcg tcccagttga
     6301 cctctccggc gtacagcacg gcgttgcgcc cgcggccgac gtcgacgaat gcggcctcca
     6361 tgccgggaag gacgttctgg acgcgcgcga ggtagacgtt gccagccatc gactgctggg
     6421 cctggtggga cacgtagtgc tccacgagga tgtcgtcctc gagcacggcg atttgaacgc
     6481 gaccgtcctt ttcgcgcacc accatcgagc gcttgacgct ttcgcggcgg gcgaggaact
     6541 cggcctccgt aatagcgggc ctgcgcgtgt cgcggccctc gcggcggcgc tggcgcttcg
     6601 cctggagtcg ggtcgagccg gcaaggtcgt ccgacgcacc ggcgccgcgc gtgcgcccgc
     6661 cgcgattgcg gcgacgacgg gaggagtcgg agccaccgtc ggcgtcgtcc ggagtctcgg
     6721 agtcgcgggt ctcgcgggcc tcgccctcgt ctccgcctcc acccgagcgg gcgcgcgttc
     6781 cgcggccgcg gccgccgcgg cgacggcgcc gacgcgtgcc gccctcgccg gattcgtcgc
     6841 cgtcgccccc atcggagtcg cgaccggcac cgtcatggcc ggaggaatcg gcgtggtcct
     6901 tgacataatc cccgggcgcg tcgccctcgg acgcggccgc ggcggcgggc ttcttcgccg
     6961 gggcccgctt ggccggcgcc ttcttc
//
LOCUS       DASVKP010000163         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_549043, whole genome shotgun sequence.
ACCESSION   DASVKP010000163 DASVKP010000000
VERSION     DASVKP010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549043"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1725)
                     /locus_tag="VF362_03360"
     CDS             complement(<1..>1725)
                     /locus_tag="VF362_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX7589998.1"
                     /translation="SIGLDDGDRVGVVGENGAGKSSLLRLLAGTIEPHGGRVTRSRGL
                     TIGMVDQASAQLRDATVASAIVGDAAVHEWASDPKIRDIMRGLVPDLPADTPLSALSG
                     GQRQRVFLARTLVQPGDVLFLDEPTNHLDLDGVAWLADHVRGRWPAGTGALVVVTHDR
                     WFLDEVSNVTWEVHDGAVERYEGGYAAYILQRVERARVSSATERRRQNLLRKELAWLR
                     RGAPARTSKPKFRIDAANELIADVPPVRNQAALVRLAAARQGKEVVSLLECSVSYGTG
                     EDAREVLRDVTWRIGPGERTGILGENGAGKSTLLNLVTGALEPTSGRVRRGINTRVAT
                     LTQHAESLSDRPDATLNAMVKGLGTTTLADGSVMRPDDLLERLGFTAAQRLTAVGRLS
                     GGQRRRLQFLLVMLSEPNVLVLDEPTNDLDTDVLAAMEDLLDEWPGTLVVVSHDRYLL
                     ERITDQQYAVVGGAFRHLPGGVEEYIALSRDRRSSGGGGMGTFGVADSSGGGGGATRR
                     AEDKESAAIERRLTKIGALKVSLHEQMVAHDQADYRGLEGLNSRLKALGDEESRLERR
                     WLEIADARG"
BASE COUNT          248 a          597 c          607 g          273 t
ORIGIN      
        1 ccccgcgcgt cggcgatctc tagccaccgc cgctcaagcc gtgactcctc atcgccgagg
       61 gccttgaggc gcgagttgag gccctcgagg ccgcggtagt cggcctgatc gtgggccacc
      121 atctgctcgt gcaggctcac cttcagggcg ccgatcttcg tcaggcgcct ctcgatcgcc
      181 gccgactcct tgtcctcggc tcggcgcgtg gccccgccgc cacccccgga ggagtccgcg
      241 acgccgaacg ttcccatccc gccaccgcca ctcgaccgac gatcgcgcga gagggcgatg
      301 tactcctcga cgccccccgg aaggtggcgg aacgcgcccc cgaccaccgc gtactgctgg
      361 tccgtgatgc gttcgagcag gtatcgatcg tggctcacga cgacgagggt ccccggccac
      421 tcgtcgagca ggtcttccat ggcggcgagc acgtccgtat cgaggtcgtt cgtcggctcg
      481 tcaaggacga ggacgttcgg ctcggagagc atcacgagga gaaactggag cctgcgcctc
      541 tgccccccgc tgaggcgccc cacggccgtg agtcgctgcg ccgccgtgaa tccaaggcgt
      601 tcgagcaggt cgtcggggcg catcaccgag ccgtccgcga gcgtggtcgt gcccaggccc
      661 ttcaccatgg cgttgagagt cgcgtctgga cgatctgaga ggctctccgc gtgctgcgtg
      721 agggtcgcca ctcgcgtgtt gatgccgcgc ctcacacggc cgctggtggg ctcgagggcg
      781 ccggtgacga ggttcagtag cgtggacttg cccgcaccgt tctctccaag gattccggtg
      841 cgctcgccag gtccgattcg ccacgtcacg tcgcgaagca cctcgcgtgc gtcctcgccc
      901 gttccgtacg agacggagca ttcgagaagc gagacgacct ccttgccctg ccgcgccgcc
      961 gcaagccgaa cgagggcggc ctggttgcgc acgggcggga cgtcggcgat gagctcgttc
     1021 gcggcatcga tgcggaactt gggcttggag gtgcgcgccg gggcgccgcg ccggagccag
     1081 gcgagctcct tgcggagaag gttctggcgt cggcgttccg tggcggagga cacccgcgct
     1141 cgctccacgc gttggaggat gtaggccgcg tagcctccct cgtatcgttc gacggcgccg
     1201 tcgtggacct cccacgtgac gttcgaaacc tcgtcgagga accagcggtc gtgggtcacg
     1261 acgacgagcg cccccgtgcc cgccggccac cggccccgca cgtggtcggc gagccaggcg
     1321 acgccgtcga ggtcaaggtg attggttggc tcgtcgagga acaggacgtc gccgggctga
     1381 acgagcgtgc gcgcaaggaa gacccgttga cgctggccgc ccgagagggc ggagaggggg
     1441 gtatcggcgg gcaggtcggg aacgagtccc cgcatgatgt cgcggatctt ggggtcggag
     1501 gcccattcgt ggacggcggc gtcgccgacg attgcgctcg cgaccgttgc gtcgcgcaac
     1561 tgcgcggagg cctggtcaac catgccaatc gtcagaccgc gggagcgggt cacccggccg
     1621 ccgtgcggct cgatggttcc cgcgagaagt ctcaggagcg acgacttgcc agcgccgttc
     1681 tccccgacca cgccgactcg atcgccgtcg tcgaggccga tagag
//
LOCUS       DASVKP010000164         2910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_470215, whole genome shotgun sequence.
ACCESSION   DASVKP010000164 DASVKP010000000
VERSION     DASVKP010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470215"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VF362_03365"
     CDS             complement(<1..907)
                     /locus_tag="VF362_03365"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7589999.1"
                     /translation="MTSSRDASPRSGLAPETFSPTIRPADDFFRYVNGPWLATHVIPD
                     DRAGDGHFYRLRDLSEERVRAIIEDAPEDSQIGALYRSFMDEERCDAVGTAPLGPDLA
                     LVDAAETSAALAVALGTLARTGIAGVISREVWADPGDPDRMVLWLGQGGIGLPDEAYY
                     REGQHKDVRDKYVAHVDRMAELAADALGARTFTGAGVLAFESAVAKHHWDIVKSREAD
                     LTYNPTTLDALATTTPGFPWRAWADALGVPEGGRDKLVAQQPSFFAELADLWAGGEAG
                     LDLDAWKAWLRWRIVSGRSPYLTREL"
     gene            981..1427
                     /locus_tag="VF362_03370"
     CDS             981..1427
                     /locus_tag="VF362_03370"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HEX7590000.1"
                     /translation="MRLHIAADHAGYELKRLLVTYLEAGGHEVIDHGAYEYDEVDDYP
                     GFCFAAGEAVAQDPDSLGIVLGGSGNGEQIAANKVKGVRAALAWSVQTAMLGRAHNNA
                     NVVALGWRQHGLVEATEIIEAFISTAFSGEERHERRIEQIAKYEAR"
     gene            complement(1554..2828)
                     /locus_tag="VF362_03375"
     CDS             complement(1554..2828)
                     /locus_tag="VF362_03375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7590001.1"
                     /translation="MIGPVIAPSGYRLVDLPRERGQEMLDIDSWAFVFAVRPSEVEAV
                     LDAFPFDRARGIEIVDPARGTPGTLAAVHSSFEFATRVPGGGTVPTAGLTWVGVHQGH
                     RRRGLLTAMITDHFARSRARGEVVSTLFAAEPGIYQRFGYGLAARDLRMKLGRKPELR
                     DVSGSDDLTVTLESSSLEAHGPVVRAFQASQLRPGTNTVVPDSLLTSLVVDVESNREG
                     AEPLRFATVRDGETVVAWATFRRKGDWEGSEPNGIVKVIAWGAMTAAATRRLWSVLTD
                     LDLMTSTRSWAMAVDDPLVHLLVDERAAKPDVRDNVWVRILDVKGAIEGRRYESDADV
                     VLRIADVQVPENAGLWRVTIADGAAHVSPDAGSSADLTLGIQELGAAYLGGVGVEALV
                     QASLVVENTPGAAKALGRAMLSALAPVSNFMF"
BASE COUNT          453 a          997 c         1053 g          407 t
ORIGIN      
        1 agagctcgcg ggtcagatac ggagaccgtc ccgacacgat gcgccaccgc agccacgcct
       61 tccaggcgtc gaggtcgagg cccgcctcgc cccccgccca gaggtcggcg agttccgcga
      121 agaacgaggg ctgctgcgcc acgagcttgt cgcgcccgcc ctcggggacg ccgagcgcgt
      181 cggcccacgc gcgccacggg aagccgggtg tcgtcgttgc gagggcgtcg agcgtcgtcg
      241 ggttgtaggt caggtctgcc tcgcgggact tcacgatgtc ccagtggtgc ttcgcgaccg
      301 cggactcgaa cgccaggacg ccggcgccgg tgaaggtgcg cgcgccgagg gcgtcggcgg
      361 cgagttccgc catgcgatcc acgtgggcga cgtacttgtc ccgcacgtcc ttgtgctgcc
      421 cttcacggta gtaggcctcg tcgggaagcc cgatgccccc ctggccgagc cacaggacca
      481 tgcgatcggg gtccccggga tccgcccaca cctcgcggga gatgacgcca gcgattcctg
      541 ttcgggcgag cgttccgagg gccacggcga gcgcagcgga cgtctccgcg gcgtcgacga
      601 gcgcgaggtc ggggccgagg ggcgcggtcc ccaccgcgtc gcagcgttcc tcgtccatga
      661 agcttcggta gagcgcgccg atctgggagt cttctggcgc atcctcgatg atggcgcgca
      721 cgcgctcctc ggagagatcg cggagtcggt agaagtgccc gtctcccgcc cgatcgtccg
      781 ggatcacgtg cgtggcgagc cacgggccgt tgacgtagcg gaagaagtcg tcggcggggc
      841 ggatcgtcgg ggagaaggtt tccggggcga gtccggatcg gggggaggcg tcgcgggagg
      901 atgtcatgcg ccaagcctag gtgagcgacg caccgagcca ccgccgccgg ggcgcccggc
      961 gggctggcag gatgggcctc atgcgcctcc acattgcggc agatcacgcg ggttacgaac
     1021 tgaagcgact tctcgtgacg tacctggagg ccgggggcca cgaggtcatt gatcacgggg
     1081 cgtatgagta cgacgaggtc gacgactatc ccggcttctg cttcgcggcg ggcgaggccg
     1141 tcgcccagga ccccgattcg ctcggcatcg tcctcggcgg atcgggcaac ggcgagcaga
     1201 tcgcggccaa caaggtcaag ggcgtgcgcg cggccctcgc ctggagcgtt cagaccgcga
     1261 tgctcgggcg cgcgcacaac aacgcgaacg tggtcgcgct cgggtggcgc cagcacgggc
     1321 tggtcgaggc caccgagatc atcgaggcgt tcatctccac ggcgttctcc ggcgaggaac
     1381 gccacgagcg gcgcatcgag cagatcgcca aatacgaggc gcgctagccc cggtcctccc
     1441 gcgacgcttg gacggggtgg tacgacgtcg cggccggacg tggtggcaga acgccgcggc
     1501 aggacgccgc aaccgagtgc cctgcgctgg acgcgggcta gtctccgccc gcctcaaaac
     1561 atgaagttcg agacgggcgc cagcgccgac agcatcgcgc ggccgagggc cttcgccgcg
     1621 cccggcgtgt tctcgacgac gagcgaagcc tggacgagcg cctcgacgcc cactccgccg
     1681 aggtatgcgg cgcccagttc ctgaatgccg agggtaaggt cggccgacga cccggcgtcg
     1741 ggcgagacgt gcgccgcgcc gtcggcgatc gtgacccgcc agagcccggc gttctcgggg
     1801 acctgcacat ccgcgatccg cagtaccacg tcggcgtccg actcgtaccg ccgcccctcg
     1861 atcgccccct tgacgtcgag gatgcggacc cagacgttgt cgcgaacgtc gggcttggcc
     1921 gcgcgctcgt ccaccaggag gtgcacgagc gggtcgtcga cggccatcgc ccacgaacgg
     1981 gtggaggtca tgaggtcgag gtcggtgagg accgaccaca gtcgccgtgt cgccgcggcc
     2041 gtcatcgccc cccacgcaat gaccttcacg attccgttcg gctccgaccc ctcccaatcg
     2101 cccttgcgcc ggaacgtcgc ccaggcgacc acggtctcgc cgtctctcac cgtggcgaag
     2161 cgcaacggct cggccccctc gcggttcgac tcgacgtcga cgaccaggct ggtgagcagc
     2221 gaatcaggga cgaccgtgtt cgttcccggg cgaagttgac tggcctggaa tgcgcgcacg
     2281 acgggtccgt gcgcctcgag cgaggaactc tcgagcgtca cggtgaggtc gtccgaaccg
     2341 gaaacgtcgc ggagttcggg cttgcgcccg agtttcatcc gcaggtcgcg cgcggcgagc
     2401 ccgtacccga agcgctggta gatcccgggc tccgccgcga agagggtact caccacctcg
     2461 ccgcgggcgc gggaacgcgc gaagtggtcc gtgatcattg cggtgaggag gccgcggcgc
     2521 cggtgcccct ggtggacgcc cacccaggtg aggccggccg tcgggacggt cccgccgccc
     2581 ggcacccgcg ttgcgaactc gaatgaggag tgcaccgcgg cgagcgtgcc gggggtgccc
     2641 cgggcggggt cgacgatctc tatcccgcgg gcgcggtcga aggggaaggc gtcgaggacc
     2701 gcctcaacct cggagggccg cacggcaaag acgaaggccc atgagtcgat gtcgagcatc
     2761 tcctgcccgc gctcgcgggg gagatcaacc aggcggtatc cggacggcgc tatcacgggt
     2821 ccgatcatgt cagcagggca acccttcgca ccacgtcatc agggcacgat tgcgcaacaa
     2881 tcgtgcgccg acgaaggcgg gagaggctac
//
LOCUS       DASVKP010000165         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_549651, whole genome shotgun sequence.
ACCESSION   DASVKP010000165 DASVKP010000000
VERSION     DASVKP010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549651"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VF362_03380"
     CDS             complement(<1..192)
                     /locus_tag="VF362_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590002.1"
                     /translation="MRVRRKPLRTPLAGLAPVFHRIREAAGVHEGYAPDVYFEAEHGG
                     GASEPPHVTARRLDRTSLPL"
     gene            complement(292..879)
                     /locus_tag="VF362_03385"
     CDS             complement(292..879)
                     /locus_tag="VF362_03385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007271093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7590003.1"
                     /translation="MTDTSDLTIAVILGSTRPGRRGAAVADWVVERAADRGATYELVD
                     LADHPLPFFDEVNPPSKHQYQHPHTIEWAETIARFDGFIFVSPEYNHSTSAVLKNSLD
                     YLNLEWNDKAAGIVTYGMAGGHRAAEALRTVLSELQVATVQRQVGLSLLTDFENFSEL
                     RPSELQVASLDSLFPQLEAWAAALKTVRAGASLTE"
     gene            complement(995..2152)
                     /gene="cax"
                     /locus_tag="VF362_03390"
     CDS             complement(995..2152)
                     /gene="cax"
                     /locus_tag="VF362_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX7590004.1"
                     /translation="MTDRKVAETGEARPRETASTPGDPARPRRLGPRDVQLLSACAIL
                     TVASGTLAKADAGPLMPFIVSAVALALLASLVGRSVDALGDRLGAGATGVVQSALGNL
                     PELFVCIFALKAGLYDVVRAAIVGSILSNVLLVLGLAFVFGGLRHGRQRFSTDRSRTL
                     ALMLLLAVFALAIPSLTFALHTPAALHEEALSQVTAIVLIALFALSLPAALAKGAIEE
                     TRREKHAAAEAASVDRWPLWLALGVLAAAAVAAAFVSDWFVTALTPAMESAHISATFA
                     GLVIVAIAGNAVENVVGIQLALRNKADYALAVILQSPLQVALVVAPALVLAAPLIGAN
                     FTLVLSPLLLAALLVSVIVTFVVVFDGESTWFEGAALVSLYVLLAAGVWWG"
     gene            complement(2227..3054)
                     /locus_tag="VF362_03395"
     CDS             complement(2227..3054)
                     /locus_tag="VF362_03395"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEX7590005.1"
                     /translation="MAARRERRAVRREGRRSSRRERATRRAPGFWFGVGLLRRGFRTW
                     RSDPGIMLLGLVPGVISSVLIAALVATFAINVDPITRWVASFANGWPEVLQQLVQALV
                     AIGLIWAIGLTIVYGFTGFTLLIGQPFFEAISRRIDDPLGAAPAAPERPWLVALVGNI
                     GERVGRLALAGLASGGLFLVSLIPVVGTGIAWTLGSLIGGWFLALELTDYPFERRGHR
                     LRYRRLALGRRRRVTLGFGIAAFLLFLVPGGAVLTMSAAVAGGTLLTRHTLGEPIAR"
BASE COUNT          562 a         1088 c         1192 g          407 t
ORIGIN      
        1 gaggggaagc gaggtccggt cgaggcgcct cgccgtgacg tgggggggct cggaagcccc
       61 gcccccgtgc tccgcctcga agtagacgtc gggcgcgtag ccctcatgga cgccggcggc
      121 ctcccggatg cggtggaaga ccggagcgag cccggcaagg ggggtccgaa ggggcttgcg
      181 gcgaacgcgc ataggacgag cctaggcgga gcctggcggc ccgatgaccg cccgccgcgc
      241 ccgttgggcg gccaccgccg cggcagccag ggcaccgcga acgctcccgc gctactccgt
      301 gagggaggcc cccgcgcgga ccgtcttgag ggcggcagcc catgcctcga gttgggggaa
      361 gaggctgtcg agcgacgcga cttggagttc cgagggccgc agttcgctga agttctcgaa
      421 atccgtcagg agcgagagcc ccacctggcg ctgaacggta gcgacctgga gttccgacag
      481 gacggtgcgc agggcctccg cggcccggtg cccccccgcc atcccgtagg tgacgattcc
      541 ggcggccttg tcgttccact cgaggttcag gtagtccagg gagttcttga gcacggcgct
      601 cgtggagtgg ttgtactcgg ggctcacgaa gatgaacccg tcgaaccgtg cgatggtctc
      661 cgcccactcg atcgtgtgcg ggtgctggta ctggtgcttc gacggcggat tgacctcgtc
      721 gaagaacggg agcgggtgat ccgcgaggtc cacgagttcg tacgtcgctc ccctgtcagc
      781 ggcgcgctca acgacccagt cggcgaccgc cgctccgcgc cggccgggac gcgtcgagcc
      841 caggatcacc gcgatggtca gatcggatgt gtcagtcacg tcggcctcct tcggaggagc
      901 cagccggtgc ggcgtgcccg tcaccaccct caacgcgttg acgtatcaac gctattcccg
      961 cggcacgacg gcgggcctcg cgcgtcgggc ggaactaccc ccaccaaacc ccggcggcga
     1021 ggagcacgta gagcgagaca agcgccgccc cctcgaacca tgtcgactcg ccgtcgaaca
     1081 cgacgacgaa ggtgacgatg acagagacga gcagggcggc gaggagtagc ggcgacagga
     1141 cgagggtgaa gttcgctccg atcagggggg cggcgaggac gagggcggga gcgacgacca
     1201 gcgcgacctg gagcggggac tggaggatca cggcgagcgc gtagtcggcc ttgttcctga
     1261 gcgcgagctg gatcccgacg acgttctcta cggcgttgcc cgcgatggcc acgatgacga
     1321 ggccagcgaa cgtcgcggaa atgtgcgccg attccatcgc cggggtgagg gcggtgacga
     1381 accagtcgga cacgaacgcg gcagcgacgg ccgccgccgc cagtaccccg agggccagcc
     1441 acaagggcca gcggtcgacg cttgccgcct ctgcggcggc gtgcttctcg cgtcgggtct
     1501 cctcgatagc ccccttggcg agggccgccg ggagggagag ggcgaagagg gcgatgagca
     1561 cgatggccgt cacctgagac agggcttcct cgtggagcgc ggccggcgtg tgcagggcga
     1621 aggtcagcga cggaatcgcg agggcgaaca cggcgagcaa gagcatgagc gcgagcgtcc
     1681 gcgaccgatc cgtggagaag cgttgccgac cgtgcctgag gccgccgaag acaaaggcga
     1741 ggccaagcac gaggagcacg ttcgacagga tcgatccgac gatggccgcg cgtacgacgt
     1801 cgtacagacc agccttgagg gcgaagatgc agacgaagag ttccggcaga ttcccgagag
     1861 ccgactggac gacccccgtc gcgcccgctc ccaaccgatc cccgagggcg tcgaccgagc
     1921 gcccgacaag gctggcgagc agggcgaggg cgacagcgct gacgatgaac ggcatcaacg
     1981 gcccggcatc agcctttgcc agcgtcccgg aggcgacggt caggatggcg cacgcgctca
     2041 agagctggac atcacgcggc cccaggcgcc tcggccgcgc cggatcgccc ggtgtcgacg
     2101 ccgtctcccg gggccgggcc tcccccgtct cagcgacctt gcgatccgtc atggagccac
     2161 tgtagggctt gcgtcagtga ccggacctct gccaggtccg cgcaaacggc cgccccaacg
     2221 ggcgacctag cgggcgatgg gctcgccgag ggtgtgtcgg gtgaggaggg tgccgcccgc
     2281 gacggcggcg gacatcgtca gcaccgcgcc gccgggaacg agaaagagaa ggaacgcggc
     2341 gatgccgaat ccgagggtca cgcgccgccg ccggccgagc gcaagcctgc ggtatctcag
     2401 gcggtggccc cggcgctcga acgggtagtc cgtcagttcg agcgcgagaa accatccccc
     2461 gatgagcgag ccgagcgtcc aggcaatccc ggtgccgacg acggggatca ggctgaccag
     2521 gaagaggccc ccgctcgcga gccccgcgag ggcgaggcgc ccgacgcgct cgccgatgtt
     2581 gccgacgagc gcgacaagcc agggacgctc tggcgccgcc ggggcggctc cgagcggatc
     2641 gtcgattcgc cgggagatag cctcgaagaa gggctgaccg atgaggaggg tgaagcccgt
     2701 gaacccgtac acgatcgtga ggccgatcgc ccagatgagg ccgatggcga cgagcgcctg
     2761 aacgagttgc tggagcacct ccggccatcc gttcgcgaaa ctcgcgaccc accgggtgat
     2821 cggatccaca ttgatggcga aggtcgcgac gagcgcggcg atgaggacgg aggagatgac
     2881 ccccgggacg aggccgagca acatgattcc cggatcgctc cgccatgtac ggaatccgcg
     2941 gcgcaggagg ccgacgccga accagaaccc gggcgcgcgc cgcgtggccc gctcgcgccg
     3001 ggaagagcgt ctcccctcgc gcctcacggc acggcgttcg cgacgagcgg ccatgggccg
     3061 atcgtatccg ggtccgcaat ggggggcgct tcccctggcc gcggtcgccc gcctagatca
     3121 gcactccgcc cgtgcgcgcc ggagtgctct cgcgcagcac gacccttccg tcgaccgtgc
     3181 gccgaatgga cgtcgcctcc tggcccctga ggatgatgtc gaccgcctcg gtcgcgacca
     3241 tctcgacgg
//
LOCUS       DASVKP010000166         3794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_798329, whole genome shotgun sequence.
ACCESSION   DASVKP010000166 DASVKP010000000
VERSION     DASVKP010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798329"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /locus_tag="VF362_03400"
     CDS             <1..1726
                     /locus_tag="VF362_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7590006.1"
                     /translation="AVPPANLGAYLRDFDVLMDRHGLHGQPYGHFGDGCVHVRLDIPL
                     GTDGSALRAFMEEAAALVSRHGGSLSGEHGDGRARSELLAAMYSPDAIRAFGEIKGLF
                     DPENLLNPGIIVDPRPLDADLRRPAAPAVKRRGGWAFEHDHGDLSEAVHRCTGVAKCR
                     GDAIEEGTGFMCPSYAATKDEKDVTRGRARVLQDAINGSLIGGLTAPEVIESLDLCLA
                     CKACSSDCPSGVDMAAYKAEILNRRYKGKIRPIMHYSLGWLPRWLKFVAIAPRTINAL
                     LKPRWIERIVVKLAGLDTRRHMPRFAPTPFRNSKTAKSRSATSRRKTTGLQTTAARGR
                     LVVLWADSFTDGLDPAIPEALVAVLEDAGFDVAVSAGDACCGITWITTGQLEGARRRM
                     EKLLGVLGPFAVNGVPIIGVEPSCMAALRSDLTELLPADPRAHAVSRGVFTLAEMLTG
                     RGPIKPRDGWKVPDLSDVTAVVQPHCHHHSVMGFGADRELLGSAGATVIEASGCCGMA
                     GMWGTTADHYDLAATVAGTSLLPALENAPEGSIYLADGYSCRTQAEDLAGVTGRHLAQ
                     LLAERGRA"
     gene            complement(1723..2922)
                     /locus_tag="VF362_03405"
     CDS             complement(1723..2922)
                     /locus_tag="VF362_03405"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX7590007.1"
                     /translation="MDDDIIESDPWASDVGPRADAPPPASAAWRDGDPPGHRSFLGIG
                     DLALDADPLGTLPDVRLAYETWGELNAAGDNAIYVAHALTGDSHVTGPAEEGHHTGGW
                     WNSMVGPGRPIDTDRYFVVCANVVGGCQGSTGPASPHPSDGKPWGSRFPYLTLRDMVR
                     AEIALADALGVSSWKLIAGPSMGGMRAIEWSIMAPGRVRAIAPVGATAATTADQIAWS
                     TSQLAAIRADPKFRGGDFYDAADGDGPHVGMGIARMIAHTTYRSEGELAARFGRAYQG
                     ASDPIGRGGKFAVQSYLEHHARKLALRFDANTYIAIVEAIQSHDVGRDRGGIEAALAD
                     VDIPALIVAVDSDRLYPVNNSELVDACLSGSHGVKMVHSDVGHDGFLVESGQLNAHMA
                     TFLASLD"
     gene            3084..>3794
                     /locus_tag="VF362_03410"
     CDS             3084..>3794
                     /locus_tag="VF362_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590008.1"
                     /translation="MRHGTGQYMQRPTGGQGAAVAARVAPRVIYACSALLLAFVTILV
                     IAPRAAAANYPGYAQVQAAKAAVNDANATVAQLDAAIAQLSDALHEADVAVLAADDAY
                     AATRQDNEQAQLQLFLANARADEADRALVAARGDLAGVARAQYRSGGQFGALEAVATS
                     TGFEDVIARTEALKRAGDTAEIAIDRVRAAELVQQTLRDFAAQASEKAIVTEKAAEDA
                     LQAAVDAAAQAKRALAETQ"
BASE COUNT          564 a         1341 c         1350 g          539 t
ORIGIN      
        1 cgccgtgccg ccagcgaacc tcggcgccta cctgcgcgac tttgacgtcc tcatggatcg
       61 gcacgggctc cacgggcagc cctacggcca cttcggagac gggtgcgtcc acgtccgcct
      121 ggacatcccg ctcggcaccg acggctccgc cctgcgcgcc ttcatggagg aggccgccgc
      181 gctcgtttcc cggcacggag gttctctctc cggcgagcat ggcgacgggc gcgcgcgttc
      241 cgaactcctc gccgcgatgt acagccccga cgcgatccgc gccttcggcg agatcaaggg
      301 tctcttcgac ccggagaacc tcctcaaccc cggcatcatc gtggacccgc gccccctgga
      361 cgcggacctg cgccggcccg ccgcgcccgc cgtcaagcgc aggggcgggt gggcgttcga
      421 gcacgaccac ggcgacctgt ccgaggcggt gcaccggtgc acgggcgtcg ccaagtgccg
      481 cggggacgcg atcgaggagg gcacgggctt catgtgcccg agctacgccg cgaccaagga
      541 cgagaaggac gtgacgcgcg gccgcgcgcg cgtgctccag gacgcgatca acggctcgct
      601 catcggcggg ctcacggccc ccgaggtcat cgagtcgctc gacctctgcc tcgcgtgcaa
      661 ggcgtgctcg tccgactgcc cgtccggcgt ggacatggcc gcgtacaagg ccgagatcct
      721 caaccgtcgc tacaaaggca agatccgccc gatcatgcac tactcgctcg ggtggctccc
      781 gcgctggctc aagttcgtcg ccatcgcgcc tcgcaccatc aatgcacttc tcaagccgcg
      841 ctggatagag cgcatcgtcg tgaagctcgc ggggctcgac acgcgtcgtc acatgccccg
      901 cttcgcccct acgccgttcc gcaacagcaa gaccgcaaag tcgcgcagcg cgacgtcgcg
      961 ccgcaagacg acgggattgc agacgacggc cgccaggggc cgactcgtcg tgctctgggc
     1021 cgactccttc accgacgggc tcgaccccgc catccccgag gccctcgtcg cggtgctcga
     1081 ggacgccggc ttcgacgtcg ccgtctcggc cggcgacgca tgctgcggca tcacgtggat
     1141 cacgacgggc cagctcgagg gagcccggag gcgcatggag aaactcctgg gcgtgctcgg
     1201 ccccttcgcc gtcaacggcg tgccgatcat cggcgtcgag ccctcgtgca tggccgccct
     1261 tcgctcggac ctcacggaac tgcttccggc cgacccgcgc gcccacgccg tctcgcgcgg
     1321 agtcttcacg ctcgccgaga tgctcacggg tcgcggcccc atcaagcccc gcgacggctg
     1381 gaaggtgccc gacctctcgg acgtcaccgc cgtcgtccag ccgcactgcc accaccactc
     1441 ggtcatgggc ttcggcgcgg accgcgagct gctcgggtct gcgggcgcga cggtcatcga
     1501 ggcgtcgggc tgctgcggca tggccgggat gtggggcacg acggcggacc actacgacct
     1561 cgcggcgacc gtcgcgggca cgtcccttct tcccgcgctc gagaacgcgc cggagggctc
     1621 gatctacctc gccgacggct actcgtgccg cacgcaggcg gaggacctgg cgggagtgac
     1681 ggggcggcac ctggcccagc tcctcgcgga gcgtggccgc gcctagtcga gcgaggcgag
     1741 gaacgtcgcc atgtgcgcgt tcagttggcc cgactccacc aggaacccgt cgtggcccac
     1801 gtccgagtgg accatcttca cgccgtgcga accgctgagg cacgcgtcga cgagctcgga
     1861 gttgttgacg gggtagaggc ggtcggagtc cacggcgacg atcagggcgg ggatgtccac
     1921 gtcggcgagc gccgcctcaa ttccgcccct gtcgcgcccg acgtcgtggg attggatcgc
     1981 ctcgacgatc gcgatgtagg tgttcgcgtc gaagcgcagc gcgagcttgc gggcgtggtg
     2041 ctcgaggtag gactgcaccg cgaacttccc gcctctgccg atcgggtcgg acgctccctg
     2101 gtaggcgcgc ccgaagcgcg ccgcgagctc gccctcggag cgataggtcg tgtgcgcgat
     2161 catccgcgcg atgcccattc ccacgtgggg cccgtcgccg tcggccgcgt cgtagaagtc
     2221 gccgccgcgg aacttggggt ccgcgcggat ggcggccaac tgggacgtcg accacgcaat
     2281 ctggtcggcg gtcgtggccg cggtcgcgcc gacgggggcg atggcgcgaa cgcgccctgg
     2341 cgccatgatc gaccactcga tcgcccgcat gccgcccatg gagggcccgg cgatgagttt
     2401 ccacgacgac acgccgaggg catcggcgag cgcgatctcc gcgcgcacca tgtcccgcag
     2461 ggtcaggtag gggaagcgcg agccccacgg cttgccgtcc gacgggtgtg gggacgcggg
     2521 ccccgtggag ccctgacagc cgccgacgac gttcgcgcag acgacgaagt agcggtccgt
     2581 gtcgatgggg cggccggggc cgaccatcga gttccaccat ccgccggtgt ggtgtccctc
     2641 ctccgcgggt ccggtgacgt gggagtcgcc cgtcagcgcg tgggcgacgt agatcgcgtt
     2701 gtcgcccgcg gcgttcagtt cgccccaggt ctcgtaggcg aggcgcacgt ccggcaacgt
     2761 gccgagcgga tccgcgtcga gggcgaggtc gccgatgccg aggaaggaac ggtgaccggg
     2821 cggatcgccg tcgcgccacg cggccgatgc gggcgggggt gcgtccgctc gcgggcccac
     2881 gtcagaggcc cacggatccg attcgataat gtcgtcatcc atgccgatca ccaccctagg
     2941 ggaatggggc cgggaccgag gacgtgcgcg cggtgcaaga gcggcgaagc ggccgggacg
     3001 gacaacggtg atgaatctca catccgtgag gcgctccccc tgcttaccat cggacataag
     3061 ggacgcgaca tcggaagggt agagtgcggc acggaacggg gcagtacatg caaaggccta
     3121 ccgggggtca gggagccgcc gttgcggcgc gcgtcgcgcc gcgggtgatc tatgcctgtt
     3181 cggccctcct gcttgccttc gtgacgatcc tcgtcatcgc gccgcgcgcc gcggcggcga
     3241 actaccccgg ctacgcgcag gttcaggccg cgaaggccgc cgtcaacgac gccaatgcga
     3301 ccgtcgccca gttggatgcg gcaatcgctc agttgagcga tgcgcttcac gaggcggacg
     3361 tcgccgtgtt ggccgccgac gatgcgtacg cggcgacgcg gcaggataac gagcaggcgc
     3421 aactccagtt gttcctggcg aacgcgcgcg cggatgaggc ggaccgggcg ctcgtggccg
     3481 cccgcggcga ccttgccggg gttgcgcgtg cgcagtaccg ctcgggcggc caattcggcg
     3541 cgctcgaggc ggtcgcaacc tcaacggggt tcgaagatgt gatcgcgagg acggaggccc
     3601 tcaaacgggc gggagacacg gcggagattg cgatcgaccg cgttcgcgcg gcggagttgg
     3661 tccagcagac ccttcgtgac ttcgcggcgc aagcgtcgga gaaggcgatc gtgaccgaga
     3721 aggcggccga ggacgccctt caggcggcgg tcgacgcggc cgcccaggcc aagcgggccc
     3781 tcgcggaaac ccag
//
LOCUS       DASVKP010000167         1902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_220114, whole genome shotgun sequence.
ACCESSION   DASVKP010000167 DASVKP010000000
VERSION     DASVKP010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220114"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VF362_03415"
     CDS             complement(<1..322)
                     /locus_tag="VF362_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017833377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX7590009.1"
                     /translation="MPSAHPKRVLLAAPRGYCAGVDRAVIAVEKALDLHGAPVYVRKE
                     IVHNKFVVETLAARGAVFVDDTDEVPEGARVVFSAHGVSPAVHAAAAARSLVTIDATC
                     PLVTK"
     gene            352..1584
                     /gene="xseA"
                     /locus_tag="VF362_03420"
     CDS             352..1584
                     /gene="xseA"
                     /locus_tag="VF362_03420"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX7590010.1"
                     /translation="MNETLPETAGDTTPERPWPVRHLTQKVKEYVDRMPPVWVEGQVL
                     NLRRWKHLAFLTLRDVEADASMSATLPYAEVEAVAGGLADGARVIVHAKPTWWTKSGS
                     LQLDARELRTVGLGELLARLEALRERLAAEGLFDDDRKVPLPFVPNLVGLVCANQGDA
                     EHDVVVNARERWPSVRFEIRRVTVQGATCVPETTAAIRELDAHPGVDVIVVARGGGSL
                     EDLLPFSSEALIRAVAECATPLVSAIGHEKDSPLLDLVADLRASTPTDAGGRIVPDAV
                     AEGRAVVRATEALRAAIEARLDREAATLMAYLTRPVLVHPDRLVEPLAEEITRHRSAA
                     RRAHGALLRAMDGHLAGLAANLRALSPQSTLDRGYSVVRGASGAAVRDAAGLEAGERV
                     AIRFARGAAEAEVIATMP"
     gene            complement(1671..>1902)
                     /locus_tag="VF362_03425"
     CDS             complement(1671..>1902)
                     /locus_tag="VF362_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590011.1"
                     /translation="PRVDIPVEMREMLAQDKSAEVLLALIQCPAVPEKILSRLARSWN
                     REVASAAKERQRNLKMSGAVASAVGQVGFASS"
BASE COUNT          275 a          676 c          672 g          279 t
ORIGIN      
        1 ccttggtgac gagcgggcag gtcgcgtcga tcgtcacgag cgacctcgcg gccgccgcgg
       61 cgtggaccgc cggggagacc ccgtgcgccg agaacacgac ccgcgcgccc tcgggcacct
      121 cgtccgtgtc gtccacgaag accgccccgc gcgcagcgag cgtctcgacg acgaacttgt
      181 tgtggacgat ctccttgcgc acgtagaccg gtgcgccatg caggtcgagc gccttctcga
      241 cggcgatgac cgcccggtcg acgcccgcgc agtatccgcg gggggcggcg agaaggacgc
      301 gcttggggtg ggcgctgggc atactcccat cgtacgtggc accctgaggc tgtgaatgag
      361 accctgcccg agaccgcggg cgacaccacc cccgagcggc cgtggcccgt ccggcacctc
      421 acgcagaagg tcaaggagta cgtcgaccgg atgccgcccg tctgggtcga ggggcaggtg
      481 ctcaacctcc ggcggtggaa gcacctcgcg ttcctgaccc tgcgcgacgt cgaggccgac
      541 gcgtcgatgt ccgcgacact cccctacgcg gaggttgagg ccgtcgcggg aggcctcgcc
      601 gacggcgccc gcgtcatcgt tcacgccaag ccaacctggt ggaccaagag cggctcgctt
      661 cagctcgacg cgcgcgaact gaggacggtg ggccttggag agctcctcgc gcggctcgag
      721 gccctccgcg agcgcctcgc ggccgaggga ctcttcgacg acgaccgcaa ggtgcccctg
      781 cccttcgtcc ccaatctcgt cgggctcgtg tgcgccaacc agggcgacgc cgagcacgac
      841 gtcgtggtca acgcgaggga gcgctggccg tcggtccggt tcgagattcg gcgcgtgacc
      901 gtgcaggggg cgacctgcgt ccctgagacg acggcagcga tccgcgaact cgatgcgcac
      961 ccgggcgtgg acgtcatcgt ggtcgcccgg ggcggcggtt cgctcgagga tctcctgccg
     1021 ttcagttcgg aggcgctgat tcgagccgtc gccgagtgcg ccacgccgct cgtcagcgcg
     1081 atcggccatg agaaggactc tcccctcctc gacctcgtcg cggacctgcg cgcctcgacg
     1141 ccaacggacg cgggggggcg catcgtcccg gacgccgtgg cggaggggcg tgcggtggtt
     1201 cgggcgactg aggccctgcg cgcggcgatc gaggcgcgac tcgatcgcga ggccgcgacc
     1261 ctgatggcgt acctgacgcg ccccgtcctc gttcaccccg atcgactcgt cgagccgctc
     1321 gcggaggaga tcacgcgtca tcgctcggcc gcgcggcggg ctcacggcgc ccttctccgg
     1381 gctatggacg ggcatctcgc gggactcgcc gccaacctgc gggcgctgtc gccgcagtcg
     1441 acgctggacc gcggctattc cgtcgtgcga ggagcatccg gcgccgcggt gcgcgacgcc
     1501 gcgggacttg aggcgggcga gcgcgtcgcc atcagattcg cccggggcgc ggccgaggca
     1561 gaggtcatcg ccaccatgcc gtgaaacgaa gggggtggcc gtcggaactc cggcggccac
     1621 cctggacttt ttcggacaga tggtggaact caggtaagtt cgcctccccc tcagctggag
     1681 gcgaacccga cctgtcctac agccgacgcc acagcgccgg acatcttgag gttgcgctgc
     1741 cgctccttag cggccgatgc gacctcgcgg ttccatgacc gcgcgagccg cgacaagatc
     1801 ttctccggga ccgccgggca ttggatgagt gcaaggagaa cctccgcgct cttgtcctgc
     1861 gcgagcatct ccctcatctc aacgggaatg tcgacccgag gg
//
LOCUS       DASVKP010000168         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_880430, whole genome shotgun sequence.
ACCESSION   DASVKP010000168 DASVKP010000000
VERSION     DASVKP010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880430"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VF362_03430"
     CDS             <1..268
                     /locus_tag="VF362_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590012.1"
                     /translation="GSPSPFVQVLVTVGADSIASERASAEASMGASEDGTVIGGGSAY
                     TVANGSILGMRVGDYFVQVTYLAGDSADVRVVTTALAQDVAKAL"
     gene            complement(302..>1626)
                     /locus_tag="VF362_03435"
     CDS             complement(302..>1626)
                     /locus_tag="VF362_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7590013.1"
                     /translation="YADAVIAWGDAGGYDLENLWDVCTVAALGVPFDRAGGRRVSTLS
                     GGEQKRLALEALLRGEHEVLILDEPDNYLDVPSKRWLEEQLIASRKAILLVSHDREVL
                     SRVATKVATIERNANGNTLWIHSGTWDTYRDAREARNARMAEDLRRWDEVHADIIEEV
                     RVMRARAAISPDLASRYQAAKTKLRKFEEAGRPEPVPLAEKVSMRLQGGRTAKRAVTV
                     KALELTGLMKPFDSEIWFGDRVALLGSNGSGKSHFLRLLAAGGSDPDEEHRPVTDLVP
                     APVAHTGKVALGSRVRPGWFAQTHVHPEWRGKTLLDVLHRGDDHRAGLARSGATSVLD
                     RYGLGASAEQAFETLSGGQQARFQVLLLELAGATLLLLDEPTDNLDLESAESLEMAIA
                     AFEGAVIAVTHDRWFARSFERYLVFGEDGRVAESAEPVWDSGRVDRER"
BASE COUNT          230 a          564 c          588 g          244 t
ORIGIN      
        1 aggcagcccc tcgccgttcg tccaggtgct cgtgaccgtg ggcgccgact ccatcgcctc
       61 cgagcgtgcc tccgccgagg cctcaatggg cgcgtcggag gacgggacgg tgatcggggg
      121 cggcagcgcc tacaccgtgg cgaacgggag catcctgggg atgcgggtcg gggactactt
      181 cgttcaggtg acctatctcg cgggagattc cgcagacgtg cgcgtcgtga cgacagctct
      241 cgcgcaggac gtcgcgaagg cgctgtagcc gacggccggg agcaaagcac cgctcggcgc
      301 gctaccgctc tcggtccacg cgacccgagt cccacacggg ctcggcgctc tccgcgacgc
      361 gcccgtcctc gccgaacacg aggtagcgct cgaaggagcg cgcgaaccaa cggtcgtggg
      421 tcacggcgat gaccgcgccc tcgaaggcgg cgatggccat ctcgagcgac tctgcggatt
      481 cgaggtcgag gttgtcggtg ggctcgtcca gcaggagcag ggtggccccg gcgagctcca
      541 gaaggagcac ctggaatcgc gcctgctggc cgccggacag ggtctcgaac gcctgctccg
      601 ccgacgcgcc aagcccgtag cggtcgagca cgctcgtggc gcccgagcgg gcgaggcccg
      661 cgcggtggtc gtcgccgcgg tggaggacgt cgaggagcgt cttgcccctc cattccgggt
      721 gaacgtgcgt ctgggcgaac cagcccgggc gaacgcggga cccgagggcg accttcccgg
      781 tgtgggcgac gggcgcggga acgaggtccg tgacggggcg gtgctcctcg tccgggtcgg
      841 atccgcccgc cgcgaggagg cgaaggaagt gcgacttccc gctgccgttg ctcccgagga
      901 gcgcgacgcg gtcgccgaac cagatctcgg aatcgaacgg cttcatgagc ccggtgagct
      961 cgagcgcctt caccgtcacc gcgcgctttg ccgtccgccc gccctggagg cgcatcgaaa
     1021 ccttctcggc gagcgggacg ggctcgggcc taccagcctc ctcgaacttc ctcaacttgg
     1081 tcttggcggc ctggtagcgc gacgcgaggt ccgggctgat cgccgccctc gcgcgcatca
     1141 cacgaacctc ctcgatgatg tccgcgtgaa cctcgtccca gcggcggagg tcctcggcca
     1201 tgcgcgcgtt gcgggcctcg cgggcgtcac ggtaggtgtc ccaggtcccg gagtggatcc
     1261 agagcgtgtt gccgttggcg ttgcgctcga tcgtcgcgac cttggtcgcg acgcggctca
     1321 gcacctcgcg gtcgtgcgag acgagcagga tcgccttgcg cgaggcgatc agttgttcct
     1381 cgagccagcg cttgctcggg acgtccaggt agttgtcggg ctcgtcgagg atgaggacct
     1441 cgtgttcgcc gcgcaggagg gcctcgaggg cgaggcgctt ctgctcgccg cccgagagcg
     1501 tcgaaaccct ccggccgccc gcgcggtcga acggaacccc gagcgccgcg accgtgcaca
     1561 cgtcccacag gttctccaag tcgtagcccc ccgcgtcgcc ccaggcaatc acggcgtcgg
     1621 cgtagg
//
LOCUS       DASVKP010000169         3555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_798645, whole genome shotgun sequence.
ACCESSION   DASVKP010000169 DASVKP010000000
VERSION     DASVKP010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798645"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VF362_03440"
     CDS             complement(<1..906)
                     /locus_tag="VF362_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEX7590014.1"
                     /translation="MAAHPGANEPEVGLYDPAYEHDSCGVAFVATMRGSAGRDITDAG
                     LTALKNLEHRGAVGAEEQTGDGAGILTQIPDAFFRKVLDFELPPAGAYAAGIAFLDAS
                     SAGRQVASVEAAAATEGVTVLGWRDVPVNPESIGPSARAAMPAMRQVFLAREGLTGID
                     LDRVVYRVRKRAERESGVFFVSLSARTISYKGMLTTYQLEQVFPDLLDPDFITELALV
                     HSRFSTNTFPSWELAQPLRLIAHNGEINTLRGNRNWMAAREGRLVSDLLGDTADITPI
                     CTPTASDTANFDEVLELLHLGGRSLP"
     gene            complement(1023..2090)
                     /gene="lgt"
                     /locus_tag="VF362_03445"
     CDS             complement(1023..2090)
                     /gene="lgt"
                     /locus_tag="VF362_03445"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX7590015.1"
                     /translation="MTPVSIPSPPLDWEVLFQIGGFKVHAYALAILIGIFVALWVTRR
                     RWVARGGDVEAMEEIAVWAIPFGIIGGRIYHVVSTPGPYFGHDGHPLDAFKIWDGGLG
                     IWGAIAIGALGAYFGARRAHVSFSAFMDAVAPGVLIAQAIGRLGNYFNQELFGAPTTM
                     PWGLEINPASPTFPNAYAPGTLFQPTFLYELVWNLAGAALLIYLDRRFKFRFGRMFWL
                     YVMVYTSGRLWIEMLRIDAAVHVYGVRINVWVAIGTLLLGVGMFLGLGLRHRGQTDDV
                     PARTLPSAPVAVAAKGAADKVSTKAPAGGDAKSGKKTKVDGAEGSAAKSSAATAKKPS
                     AQAPEKVAATAPSAETKAKKP"
     gene            complement(2087..2887)
                     /gene="trpA"
                     /locus_tag="VF362_03450"
     CDS             complement(2087..2887)
                     /gene="trpA"
                     /locus_tag="VF362_03450"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX7590016.1"
                     /translation="MTALSDRLAEIKTEGRAALIGYLPVGYPSVQGSIAAMVAMVEAG
                     VDAVEVGIPYSDPVLDGPVIQRAAEAALASGTRVSDAFLAVRAVVDAGAPAVVMSYYN
                     PMLQYGLERFATDLAAAGGSGAITPDLTPDVGGAWITAATARGLDRIFLVAPSSTPER
                     LHMTVAATTGFVYAASLMGVTGERSAVGGAAERLVARAREAGASAVCVGLGVSTGAQA
                     AQVAAYADGVIVGSAFVRPLLDVAEPEAGIEALKVKVAELAAGVRGRA"
     gene            complement(2884..>3555)
                     /locus_tag="VF362_03455"
     CDS             complement(2884..>3555)
                     /locus_tag="VF362_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7590017.1"
                     /translation="RDWVANVDTTNYVFGTAAGPHPFPAMVRDFQRVIGVEARAQMLA
                     RGGLPDAVVACVGGGSNAIGIFDAFLDDASVRLVGCEAAGDGVETGRHASAISGGRPG
                     VLHGSQSYVLQDADGQTQASHSISAGLDYPGVGPQHAWLAELGRAEYWAITDAEAMEA
                     FRLLCRTEGIIPAIESAHALAGAMKLGVELGASATILVNLSGRGDKDVETAARYFALL
                     PESTP"
BASE COUNT          590 a         1251 c         1195 g          519 t
ORIGIN      
        1 gggcaggctc cgcccgccca ggtggagcaa ctcaaggacc tcatcgaagt tcgccgtgtc
       61 cgatgccgtc ggggtgcaaa tcggcgtgat gtccgcggtg tcgcccagga ggtcggagac
      121 gagcctgccc tcgcgggccg ccatccagtt gcggttgcca cgcagggtgt tgatctcgcc
      181 gttgtgcgcg atcaaccgaa ggggctgggc gagctcccac gacgggaagg tgtttgtgga
      241 gaaccgcgag tgcaccagcg cgagttcggt gatgaaatcc gggtccagaa gatcggggaa
      301 gacctgctca agttgatatg tcgtgagcat gcccttgtag gagatggtcc gtgcggacag
      361 cgagacgaag aagactcccg actcgcgctc cgcgcgcttc cggacgcggt aaaccacccg
      421 gtcgagatcg attcccgtga gtccctcgcg cgcaaggaac acctgacgca tcgcgggcat
      481 cgcggcacgc gccgacgggc cgatggattc gggattgaca ggtacgtcgc gccaaccgag
      541 taccgtcacg ccctccgtgg ccgcggccgc ctcaacggag gccacctgac gccccgcgct
      601 cgacgcatca aggaacgcga tgccggcggc gtacgcaccc gccggcggaa gctcgaagtc
      661 caggaccttc cggaagaacg cgtcgggaat ctgagtgagg atccccgcgc catcgcccgt
      721 ctgctcctcg gcgccgacgg cgcctcggtg ctcaaggttc ttgagggcgg tcaggccagc
      781 gtccgtgatg tcccggccag ccgatccgcg catcgtcgcg acgaatgcca ctccgcacga
      841 gtcgtgctcg tacgcggggt cgtacaaacc cacttcgggc tcgttcgcgc ctggatgcgc
      901 cgccatcaca ccgtccttcg tccctctcgg cccaagccga gactgcctag gtttcggggc
      961 gccctcgtcc ctgtgaacac acgggaaact agcgtaccgc cccgccgctc gctacccgga
     1021 cttcagggct tcttggcctt tgtttccgcc gatggagcgg ttgcggcaac cttctccggt
     1081 gcctgcgcgg acggcttctt cgcggtcgcg gccgaggatt tcgccgcact tccctcggcg
     1141 ccatccactt tcgtcttctt gccggacttg gcgtcgccgc ccgcgggcgc cttggtggac
     1201 accttgtccg ccgccccctt tgcggcaacc gcgactggcg cggagggaag ggtgcgcgcc
     1261 ggcacatcgt ccgtctgccc acggtgacgg agccccagcc cgaggaacat ccccaccccg
     1321 aggagcagcg tgccgatcgc gacccacacg ttgatcctca cgccgtagac gtgcacggcc
     1381 gcgtcgatcc ggagcatttc gatccacagg cggcccgacg tgtacaccat gacgtagagc
     1441 cagaacatgc ggccaaagcg gaacttgaag cgtcgatcca ggtagatgag gagcgcggcg
     1501 cccgcgaggt tccacaccaa ctcgtagagg aacgtcggct ggaagagggt gcccggagcg
     1561 taggcgttcg ggaacgtcgg gctcgccggg ttgatctcga ggccccacgg catcgtcgtc
     1621 ggagccccga acagttcctg gttgaagtag ttcccgagac gaccaatcgc ctgagcgatc
     1681 agtaccccgg gcgcgaccgc atccatgaat gcggagaacg acacgtgtgc gcgccgcgcg
     1741 ccgaagtagg cgccgagcgc gccgatcgcg atggcgcccc agattccgag gccaccatcc
     1801 cagatcttga aggcatcgag agggtgcccg tcgtgcccga aatagggtcc gggcgtcgac
     1861 accacgtggt agatccggcc gccaatgatc ccgaagggaa tggcccaaac cgcgatttcc
     1921 tccatcgcct caacgtcgcc gccgcgcgcg acccaccggc ggcgcgtcac ccagagggcg
     1981 acgaagatcc cgatcaggat ggccaaggcg tacgcatgaa ccttgaagcc gccgatctgg
     2041 aacagcactt cccagtccag gggcggactg gggatcgata cgggtgtcat gctcgtcctc
     2101 tcacgcccgc cgctagctcg gcgaccttga ccttcagtgc ctcgatgccc gcctcgggct
     2161 ccgccacatc cagcagcggc cgcacgaatg cgctgccaac gatcacgccg tcagcatagg
     2221 ccgcgacctg ggcggcctgc gcaccggtcg agacgcccaa ccctacgcac accgcagacg
     2281 caccggcctc gcgagcccgc gcgacgagcc tctccgcggc tcctcccacg gcgctccgtt
     2341 cgcccgtcac gcccatgagc gacgccgcgt agacgaagcc cgtcgtcgcg gcgaccgtca
     2401 tgtggaggcg ctcgggggtc gaggagggtg ccaccaggaa gattcggtcg agaccgcgtg
     2461 ccgtggccgc cgtgatccac gccccgccga cgtcgggggt gaggtccggc gtgatcgccc
     2521 ccgagccgcc cgcggcggca aggtcggtcg cgaatcgctc gagcccgtac tgaagcatcg
     2581 ggttgtagta ggacatgacg acggctggcg cgcccgcatc gacaaccgcc cgcaccgcga
     2641 ggaacgcgtc ggacacgcgc gtgccggagg cgagcgccgc ctccgcggcc cgctggatga
     2701 cggggccgtc gagcacggga tccgagtacg gaatccccac ctccacggcg tcgacgccag
     2761 cctcgaccat ggcgaccatc gccgcgatcg aaccttggac cgacggatac ccgacgggca
     2821 ggtacccgat cagcgcggcg cgcccctccg tcttgatctc ggcgagccgg tcggaaaggg
     2881 cggtcacggc gtcgactccg gaagcaaggc gaagtaccgc gcggccgtct cgacatcctt
     2941 gtcgccgcgg cccgacaggt tgacgaggat cgtcgcggag gcgccgagct cgacgccgag
     3001 cttcatggct ccggcgaggg catgcgcgga ctcgatcgcc gggatgattc cctcggtgcg
     3061 gcataggagg cggaacgcct ccatcgcctc cgcgtcggtg atcgcccagt actctgcgcg
     3121 acccaactcg gccagccacg catgctgggg gccgacgccg gggtagtcga gacccgccga
     3181 gatggagtgg gaggcctgcg tctggccgtc ggcgtcctga aggacgtagg actgcgagcc
     3241 gtggaggacg ccggggcgtc ccccgctgat cgccgacgcg tggcggcccg tctccacgcc
     3301 gtcgcccgcg gcctcgcatc cgacgaggcg gacgctggcg tcgtcaagga acgcgtcgaa
     3361 gatcccgatc gcgttcgagc cgccgccgac gcaggcgacg accgcgtcgg gcaggccacc
     3421 gcgggcgagc atctgcgcgc gggcctcaac cccgatgacc cgctggaagt cgcgaaccat
     3481 ggcggggaac gggtgcggtc cggccgcggt gccgaacacg tagttggtgg tgtcgacgtt
     3541 cgcgacccag tctcg
//
LOCUS       DASVKP010000170         3182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1294715, whole genome shotgun sequence.
ACCESSION   DASVKP010000170 DASVKP010000000
VERSION     DASVKP010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294715"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1029)
                     /locus_tag="VF362_03460"
     CDS             complement(37..1029)
                     /locus_tag="VF362_03460"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX7590018.1"
                     /translation="MTAVWRSLAEVPSDFGPTVVTLGNFDGVHRGHQAVLQTLVADAR
                     AAGCRSVAVTFDPHPRAVHRPDSPPTLVTGVEDRVERLAATGLDAVIVIHYTLAFAHQ
                     TPEEFVRSCFVDGLRAATVVIGHDTTFGWGNVGHADTMRELGAALGFEVEIVEWAGIS
                     NGSDRRWSSTWVRELLEAGDVAGAARILGRPHRLRGEIVPGDALGREIGFPTANLDTY
                     AGMIPAHGVYAGWVTILEPGSSGDAAALGGQALPAAVSLGVNTTVGGTELHVEAHLID
                     IEGVDLYGARVALDLVDRRRDMQKFGSIEELTAALAEDVTWCREVLGAGVGLDA"
     gene            complement(1088..2068)
                     /gene="truB"
                     /locus_tag="VF362_03465"
     CDS             complement(1088..2068)
                     /gene="truB"
                     /locus_tag="VF362_03465"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX7590019.1"
                     /translation="MTITTRVPTTVATTTSAVAGFVVVDKPAGWTSHDVVARMRRLAG
                     TRKVGHAGTLDPMATGVLIIGIGQATRLLSFVTSADKAYDATIRLGATTVTDDAEGEV
                     TERTDASAVTPQAIAEAVAALTGDIEQVPSSVSAIKVDGKRSYARVRAGEDVQLKARP
                     VSVSGFKVGAARSGVDEGVPFLDLDVSVTVSSGTYVRALARDLGEALGVGGHLTALRR
                     TRVAGFSLADATELEELSAGVEAGRPLVAIPLADAAVGALPSRVVSADDAVNLGFGRR
                     IPRDPSDGDANAARPLALLGPEGSLVALVVTDGALLRPLAVFAADTIGAP"
     gene            complement(2022..2504)
                     /gene="rbfA"
                     /locus_tag="VF362_03470"
     CDS             complement(2022..2504)
                     /gene="rbfA"
                     /locus_tag="VF362_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX7590020.1"
                     /translation="MADSARALRVAGTIQRVVARALEAEIKDPRLGFVTVTDVRVTGD
                     VQHATIFYSVYGDEAARAETALALASANGRLRSLVGGELGIRLTPSLEFVLDAVPETA
                     ASLEKALAEAKLRDEQSATLREGATYAGEADPYRHPDSDAGDDDDDHDEGADDGGDDD
                     "
     gene            complement(2504..>3182)
                     /locus_tag="VF362_03475"
     CDS             complement(2504..>3182)
                     /locus_tag="VF362_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX7590021.1"
                     /translation="LAKRRKRISLEDFTEALAAGKVDTLNLIIKGDVSGAVEALEDAL
                     VAIDVGDEVELRVIHRGVGAVTQNDVNLATVDNAIIIGFNVRPAERVQELADREGVDM
                     RFYSVIYRAIEDIEAALKGMLKPEFVENATGSAEIVEVFRSSKFGNIAGCMVRSGTIR
                     RNAKGRLSRDGSVVAEVSIDTLRRFKDDATEVREGFECGIGLGKVNDLQVGDMIETYE
                     MVEVPRT"
BASE COUNT          478 a         1133 c         1105 g          466 t
ORIGIN      
        1 cccggtcccg gagcagctcg ggtcggcaat ggagcgttac gcgtcgagac cgacccctgc
       61 gcccagcacc tcgcggcacc acgtgacgtc ctcggcgagc gcggccgtca actcctcgat
      121 ggacccgaac ttctgcatgt cgcgcctacg gtccacaagg tcgagcgcga cgcgcgctcc
      181 gtagaggtca accccctcga tgtcgatcag gtgagcctcg acgtgcaact cggtgccgcc
      241 gaccgtggtg ttgacgccga ggctcaccgc cgcggggaga gcctgtcccc cgagcgccgc
      301 ggcatcgccg ctcgagccgg gttcgaggat ggtcacccaa cccgcgtaga ccccgtgggc
      361 ggggatcatc cccgcgtagg tgtcgaggtt ggcggtcggg aatccgatct ccctgcccag
      421 cgcgtccccg gggacgatct cgccgcgcaa ccgatggggg cggccgagga tgcgcgccgc
      481 gcccgcgacg tcacccgcct cgaggagctc gcggacccac gtggacgacc accggcggtc
      541 ggagccgttc gagatgccag cccattccac gatctcaacc tcgaagccca acgccgcgcc
      601 gagttcgcgc atcgtgtcgg cgtggccgac gttgccccat ccgaaggtgg tgtcgtgtcc
      661 gatcaccacc gtcgcggcgc gcaatccgtc gacgaagcac gaccggacga actcctcggg
      721 cgtctggtgc gcgaaggcga gcgtgtaatg gatgacgatc accgcgtcga gccccgtcgc
      781 cgcgaggcgc tcgacgcgat cctccacccc ggtgacgagg gtaggcggac tatcgggccg
      841 atggacggcc cgcggatggg gatcgaaggt aacggcgacc gaccggcacc ccgccgctcg
      901 cgcgtcggcc acgagcgtct gaaggaccgc ctggtgaccc cggtgcacgc cgtcgaagtt
      961 gcccagcgtc acgaccgtgg gaccgaagtc ggaggggacc tcggcgagcg agcgccagac
     1021 ggccgtcatc gcgcggcgcc tccgagcact gcttctccga acgcagcacc cgcgcgcgag
     1081 ggagagatca tggtgcgccg atcgtatcgg cggcgaacac ggccagcggg cgaagcaggg
     1141 cgccgtccgt gacgacgagc gccacgaggc tcccctcggg accaagcaag gcgaggggac
     1201 gcgcggcgtt ggcgtctccg tcggacggat cgcgcgggat tcggcggccg aacccgaggt
     1261 tgacggcgtc gtcggccgag accacccgcg agggcagcgc gccgaccgcg gcatccgcga
     1321 gggggatggc aacgaggggt cgccccgcct cgactccagc ggagagctcc tcgagttccg
     1381 tcgcatcggc gagggagaag cccgcgacgc gggtgcgccg cagcgcggtg aggtgccccc
     1441 ccaccccgag cgcctcgccg aggtcgcggg cgagggcccg gacgtaggtt cccgacgaga
     1501 cggtaaccga gacgtcgaga tcgaggaagg ggacgccctc gtcgacgccg gaacgcgccg
     1561 cgccgacctt gaagccgctc acgctcacgg gccgcgcctt cagctgaacg tcctcgcccg
     1621 cgcgcactcg tgcgtacgag cgcttgccgt cgaccttgat cgcgctcacc gaactcggca
     1681 cctgctcgat gtctcccgtc agggccgcga cggcctcggc gatggcctga ggagtgacgg
     1741 cggaggcatc ggtgcgctcc gtcacctcgc cctccgcgtc gtccgtcacg gtggtggcgc
     1801 cgagccgaat ggtggcgtcg taggccttgt ctgcggacgt gacgaaggac aggaggcgcg
     1861 tcgcctggcc gatgccgatg atgagaacgc cggtcgccat cggatcgagc gttcccgcgt
     1921 gccccacctt gcgggtgccg gcgagcctgc gcatgcgcgc gacgacgtcg tgggacgtcc
     1981 atcccgcggg cttgtccacg acgacgaacc ccgcgacggc gctagtcgtc gtcgccaccg
     2041 tcgtcggcac cctcgtcgtg atcgtcatcg tcatcgccgg cgtccgagtc cgggtggcga
     2101 tacggatcgg cctcccccgc ataggtggcg ccctcgcgca gggtcgccga ctgctcgtcg
     2161 cgcaacttcg cctccgcaag cgccttctcg aggctcgcgg ccgtctccgg aacggcgtcg
     2221 agcacgaact cgaggctcgg cgtgagccga atcccgagtt cgcctccgac cagggaccgg
     2281 agccgcccgt tcgcggaggc gagggcgagg gccgtctcgg cgcgcgcggc ctcgtctccg
     2341 tagacggagt agaagatcgt cgcgtgctgg acgtcccccg tcacccggac gtccgtcacg
     2401 gtgacgaagc ccaggcgggg gtccttgatc tcggcctcga gcgcgcgggc gacgaccctc
     2461 tggatcgtgc ccgcgactcg aagggctcgc gctgagtcgg ccattaggtc ctcggaacct
     2521 cgaccatctc gtaggtctcg atcatgtcgc cgacctggag gtcgttgacc ttcccgagcc
     2581 cgatgccgca ctcgaagccc tcgcggacct cggtcgcgtc atccttgaac cggcgcaggg
     2641 tgtcgatcga gacctccgcg acgacggagc cgtcgcggct gagccgtccc ttggcgttgc
     2701 ggcgaatcgt cccggagcgc accatgcacc cggcgatatt gccgaacttc gaggagcgga
     2761 agacctccac gatctctgcc gaacccgtgg cgttctccac gaactccggc ttgagcatgc
     2821 ccttgagcgc ggcctcgatg tcctcgatcg cccggtagat gaccgagtag aagcgcatgt
     2881 ccaccccctc gcgatccgcg agctcctgga cgcgctcggc ggggcgcacg ttgaagccga
     2941 tgatgatcgc gttgtccacc gtggcgaggt tgacgtcgtt ctgggtgacg gcgccgacgc
     3001 cgcggtgaat gaccctgagt tcgacctcgt ctcccacgtc gatcgccacg agggcatcct
     3061 cgagcgcctc gaccgcaccg gacacatcgc ccttgatgat gaggttgagg gtgtcgacct
     3121 tgccagcggc gagggcttcg gtgaagtcct cgaggctgat gcgcttgcgg cgcttcgcga
     3181 gt
//
LOCUS       DASVKP010000171         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1045459, whole genome shotgun sequence.
ACCESSION   DASVKP010000171 DASVKP010000000
VERSION     DASVKP010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045459"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1178
                     /locus_tag="VF362_03480"
     CDS             162..1178
                     /locus_tag="VF362_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7590022.1"
                     /translation="MPEHNPNEQAVTFTSAGVRLSGTLTTPNADGLSPAVMLLPGSGR
                     TDRDDNAKTMALNLFPELVAELSSIGFATFRYDKRGVGSSEGDYFATGFDDQLADASA
                     AVVWLAGRPEVDPKRIFVLGHSEGAMNAVRLAAGAAGAGTEAVAGIVLLGGSAKTGEE
                     TMLWQGHQIAPTITGFNKLIINLLRIDVVTSQVKGINRLKATSADVARMQGQKINAKW
                     VREFLAYDPAPDLAKVHVPVLAITGANDIQVDPDDLRLMAELIPAEVESHVVPDVTHL
                     FRADPSRGGLKDYKKQTKRPVDPRVVGYVTAWLAAHGIVSSTFPFARRDSSRSNAARK
                     SEKS"
     gene            complement(1094..2101)
                     /locus_tag="VF362_03485"
     CDS             complement(1094..2101)
                     /locus_tag="VF362_03485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX7590023.1"
                     /translation="MLAIAYVRYGGPDVTEVIDAPVPSPAADEVLIRVRAAGLNPVDA
                     YQRAGALRAISPQRLPVVGGNELSGVVEAVGERVESCRPGDAVIARTGKAKLGAFAQY
                     AVVREDIVARAPHRVDLTVAAGLPLAGLTALQALDALELRAGERILITGGAGGVGLFA
                     IRLATLRGAHVTTTASSAGEQFVRAAGAAAVIDYHTTSPSAAGETFDKVFDLVGGEEL
                     PRLFGVTRKGGLVLSIAGPVTADAIAGDLHGLRRVLVSAVLNVRTRGIRAAVRRAGVS
                     YRYLFMHPSGSQLQSLADLVDDGKLDIVIDSTYDFSDFRAAFERLESRRAKGKVLLTM
                     P"
BASE COUNT          427 a          873 c          784 g          364 t
ORIGIN      
        1 ggcagttgcg gcgccgcggg cgcaattcgg acccgccccg ggccgacggc gacggcgact
       61 cgtcgcgaac atcttcatgc cgctggcggg cgaacatgac cacgacaaga caggccatga
      121 caagacaggc gacgaacaga cagaccctga ggagacggac catgcctgag cacaacccca
      181 acgagcaagc ggtgaccttc acgtccgcag gcgtccgctt gtccggaacg ctcaccaccc
      241 ccaacgccga cggtctctcc cccgccgtca tgctgctacc ggggtcgggt cgcacggacc
      301 gcgacgacaa cgcgaagacc atggcgctca acctgttccc ggaactcgtc gcggaacttt
      361 cgagcatcgg atttgcgacc ttccgctacg acaagcgcgg cgtcggatca agcgagggcg
      421 actacttcgc caccggcttc gacgaccaac tcgccgacgc ctcggccgcc gtcgtgtggc
      481 tcgccggtcg acccgaggtt gatccgaaac ggatcttcgt cttgggccac agcgagggcg
      541 cgatgaacgc cgtgcggctc gccgccggcg cggcaggggc tggcacggaa gcggttgccg
      601 ggatcgtcct gctggggggg tcggcaaaga ccggagaaga gacgatgctg tggcagggtc
      661 atcagatcgc cccaacgatc accgggttca acaagctcat catcaacctc ctgcgcatcg
      721 acgtcgtcac atcgcaggtg aagggcatca atcgcctcaa ggcaacgtcg gcggatgtcg
      781 cgcgcatgca ggggcagaag atcaacgcga agtgggtgcg agagttcctc gcctatgacc
      841 cggcccccga cctcgccaag gtgcatgttc ccgttctcgc gatcaccggc gccaacgaca
      901 tccaggtcga cccggacgac ctacggctca tggcggaact catccccgcc gaggtcgagt
      961 cgcacgtggt gccggacgtg acccacctgt ttcgcgccga tccctcgcgc ggcggcctca
     1021 aggactacaa gaagcaaacc aagcggcccg tcgatcctcg cgtggtcggg tacgtgacag
     1081 cctggctcgc cgctcatggc atcgtgagca gcaccttccc cttcgcccgt cgcgactcga
     1141 gccgctcgaa tgccgcgcgg aagtccgaga agtcgtaggt gctgtcgatg acgatgtcga
     1201 gtttgccatc gtcgacgagg tctgcgaggc tctgcagttg tgaaccggag ggatgcatga
     1261 acaggtagcg gtagctgacg cccgccctgc ggaccgcggc gcggatcccc cgcgtgcgca
     1321 cgttcagcac cgcgctcacg aggacccgtc gcagaccatg gagatcgcca gcgatggcgt
     1381 ccgcggtgac ggggccagca atactcagga cgaggccgcc cttgcgggtc actccgaaca
     1441 gccggggaag ttcctccccg ccgacgagat cgaagacctt gtcgaaggtc tcgcccgccg
     1501 cggacggcga cgtcgtgtgg tagtcgatca ccgccgcggc gcccgcggcc cgcacgaact
     1561 gctcgcccgc cgaggacgcc gtcgtggtca cgtgtgcgcc acggagcgtc gcaagccgga
     1621 tggcaaacag gccaacgccg ccagctccgc cggtgatgag gatgcgctcg cccgctcgaa
     1681 gttcgagggc gtccagcgcc tgcagtgcgg tcaggcctgc gagcggaagt cccgccgcga
     1741 cggtgaggtc gactcggtgt ggcgcgcgag cgacgatgtc ctcccggacc acggcgtact
     1801 gggcgaacgc gccgagtttc gccttgcccg tccgtgcgat gacggcgtcg ccggggcggc
     1861 atgactcgac ccgctccccc acggcctcaa cgacgccgct caactcgttc ccgccgacga
     1921 ccgggagcct ttgcggggag atcgcccgga gggcccccgc tcgctggtac gcgtccacgg
     1981 ggttcaggcc ggcggcgcgg acgcggatga gcacctcgtc tgctgcgggc gacggcacgg
     2041 gtgcatcaat cacctcggtg acgtctggtc ctccgtaacg gacatatgcg atcgcgagca
     2101 tctgcgcgcc ctctccccac ctcgtgtgaa cagtgatgac tctctatgac agcatccctt
     2161 ctgccaccac gtcgacctcc gcgccgcgcg gccctggcgg cgaggacgct tgacgtgtca
     2221 tcggcgtcgc cgaaagcagt taacatgggc taagcatctt gtccgcgcct cgccgcccaa
     2281 gaaggaaccc atggtcacca ccgccccccc gcgcgcgctc acccgcgctg gccgcgccgc
     2341 gcgcgacacc gccaagcccc actaccgctg gatcgtcctg tccaacacga cgctcggcgt
     2401 cctcatggcg tcgatcaaca tgtccatcct gatgatcgcg ctccccga
//
LOCUS       DASVKP010000172         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_880962, whole genome shotgun sequence.
ACCESSION   DASVKP010000172 DASVKP010000000
VERSION     DASVKP010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880962"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(370..>1500)
                     /locus_tag="VF362_03490"
     CDS             complement(370..>1500)
                     /locus_tag="VF362_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX7590024.1"
                     /translation="GAKTQVLVSGTGDFVRTRLTHTLEVAQIGRELGKSLGCDPDVVD
                     TACLAHDLGHPPFGHNGERALDEVARHIGGFEGNAQTLRVLTRLEPKSVDPVTGASAG
                     LNLTRASLDAASKYPWRRADAPARLSARPTAKFGVYDDDLPVFEWLREGAPEGAVSFE
                     AQVMDLADDISYSVHDVEDSVVYNHVSLGVLRDPGVLRAVVNHAREWYGRGEEEELLA
                     AFERLTALPLWVPEFDSSHRALANLKDLTSELIGRFCGAVTDATRAAYGAGPLTRHGA
                     NLVIPLDTASEILVLKGVAVHFLMAPRDNSKSHIAQRQRLGELVLAIADRGESALEPH
                     FAASWREAVDDDARFRVAVDQVASLTDKSAYNLHHKLVTPKG"
BASE COUNT          205 a          504 c          558 g          233 t
ORIGIN      
        1 aggtcgaggg gcgccgccgt gcttgcgggg gacgtggcac cggtcgccgt ggggttcgcc
       61 gatgagcttg gcccggagcc attcccgcat cctgcgagga gggcggccag gccgacggcg
      121 accgcgagtg tcctccatgc cgtccgagct tccggcgttc caccgatgcc ttgcgcgcct
      181 acccccatga tcgacaccct tctcgcaccg ggcgggccca tcggcgcacg ggcgtgccga
      241 atccccgttt cctgactgtg gcacaagaac ggagcccagg tggctttcgt tgggggggtg
      301 accgcgattc gcgccgttcc acaggggccc agcccccgcc tccacagggg gtccacactc
      361 tgggcaggct tagcccttcg gggtgaccaa cttgtggtgg aggttgtacg ccgatttgtc
      421 ggtgagggag gccacttggt ccaccgcgac ccggaacctc gcgtcgtcgt ccacggcctc
      481 tcgccacgat gcggcgaagt ggggctcgag ggcactctcc ccccggtcgg cgatggccag
      541 gacgagctcc ccgaggcgct ggcgctgggc gatatgggac ttggagttgt cgcgcggcgc
      601 catcaggaag tggacggcga cccccttgag cacgaggatc tccgacgcgg tgtccagggg
      661 gatgacgagg ttcgcgccgt ggcgcgtgag cgggccggcg ccgtacgcgg cgcgggtggc
      721 gtccgtgacc gctccgcaga agcggccgat gagctcgctc gtgaggtcct tgaggtttgc
      781 gagggcccga tgagaactgt cgaactcggg gacccacagc ggaagtgccg tgaggcgctc
      841 gaaggccgcg aggagttcct cttcctcgcc gcgcccgtac cactcgcgcg cgtggttgac
      901 gacggcgcgc aggactcccg ggtcgcgaag cacaccaagc gagacgtggt tgtagaccac
      961 ggagtcctcg acgtcgtgga cggagtagct gatgtcgtcg gcgaggtcca tgacctgcgc
     1021 ttcgaacgac accgcgccct ctggcgcccc ctcacggagc cactcgaaca ccgggaggtc
     1081 gtcgtcgtag acgccgaact tggcggtggg ccgcgccgaa aggcgggccg gggcgtcggc
     1141 gcggcgccac gggtacttgc tcgcggcgtc gaggctcgcg cgcgtgaggt tgagcccggc
     1201 gctcgcgccg gtgaccgggt cgacggactt gggctcgagc cgcgtgagga cccggagggt
     1261 ctgcgcgttg ccctcgaagc cgccgatgtg ccgggcgacc tcgtccagcg cccgttcgcc
     1321 gttgtggccg aacggcgggt ggccgaggtc gtgcgcgagg cacgcggtgt cgacgacgtc
     1381 cggatcgcac cccaaggact tcccgagctc gcgcccgatc tgcgcgacct cgagcgtgtg
     1441 cgtaaggcgc gtgcggacga agtccccggt ccccgacacg agcacttggg tcttggcgcc
//
LOCUS       DASVKP010000173         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049 k141_55071,
            whole genome shotgun sequence.
ACCESSION   DASVKP010000173 DASVKP010000000
VERSION     DASVKP010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55071"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VF362_03495"
     CDS             <1..1219
                     /locus_tag="VF362_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX7590025.1"
                     /translation="DGRVKWIVDGFTTTDMYPYADHLAIPTGTGGPDYQMNYIRNSVK
                     ATVDAFDGTVTLYAWDPTDPVLQTWSRIYPGSLHPISEISSQLMSHLRYPEDLFKIQR
                     FELATYHVSDAASFYSGQDFWTNPVDPTVITTALQPPYYLTLQMPGQEAPTFSLTTTF
                     IPGGNSNRNVLTGFLAVDSETGSVAGAPSADYGKLRLLELPRDTTVLGPGQMQNAFNS
                     NTSAQNVLNLLRQGETDVKSGNLLTLPVGGGLLYVQPVYVQSSKGTQFPLLRKVFVAF
                     GDSVGFADTLDEALDQLFGRQVTPPNPGTTDPGTTSPGTTSPGGTTTTGPTAEALAAL
                     HQALADAQAAIIDGQAALATGDFAAYGEAQARLQAALQAAVDAEAIVNGDGAGTSATS
                     GGTVVAPSPGTAG"
     gene            complement(1223..1939)
                     /locus_tag="VF362_03500"
     CDS             complement(1223..1939)
                     /locus_tag="VF362_03500"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEX7590026.1"
                     /translation="MTTIPSAPTPPPFGGAPRARWAAGRPTGLTTATIGLTGGVAVAT
                     LFGALASRAMYANFDQYVGSNPTIDATYLASQGLSGLASLLMLGSYITLALWMSKVHR
                     RLTEAGEAQPLSEIWAWFVWLIPLASIVMPYLYFRGLNRRARSRTVGAWWLTYIASGV
                     ASMVGAAQVIAASDFSVAFKDANDPLAGIDLSPLGVAGVVSAVLLVVSWVFLAATLRA
                     ITARDVTGRDVTVRAANASS"
     gene            2011..2084
                     /locus_tag="VF362_03505"
     tRNA            2011..2084
                     /locus_tag="VF362_03505"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2045..2047,aa:Met,seq:cat)
     gene            complement(2194..2685)
                     /locus_tag="VF362_03510"
     CDS             complement(2194..2685)
                     /locus_tag="VF362_03510"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HEX7590027.1"
                     /translation="MHVEFAHRFCAPVADVVAMYADEEFSRERARATGSTDSDVLIDG
                     TADGAFDVVIRRVIATEGVRSDFRGLLGSTLSVIYTEAWTEPEGEARDATFAVEIVGA
                     PARAAGSVTLAPDGSGSRMSVEGNVSSSAFLISAAVAQAVGEALARGIEQEFDAADAW
                     LAR"
BASE COUNT          478 a          960 c          888 g          388 t
ORIGIN      
        1 cgacggtcgc gtcaagtgga ttgttgacgg cttcacgacg accgacatgt atccgtacgc
       61 cgaccacctg gcaatcccga cgggcaccgg cggccccgac taccagatga actacatcag
      121 gaactccgtc aaggcgacgg tcgatgcatt cgacggcacg gttaccctct acgcctggga
      181 cccgaccgac cccgtccttc agacgtggag ccgcatctac cccggctccc tccacccgat
      241 ctccgagatc tcctcgcaac tcatgagcca cctgcgctac ccggaggacc tcttcaaaat
      301 ccagcgcttc gaactcgcga cctaccacgt ctccgacgct gcctccttct actcgggcca
      361 ggacttctgg accaaccctg tcgatccgac cgtgatcacg acggcgctcc agcctccgta
      421 ctacctcacc ctgcagatgc cgggccagga ggctccgacc ttctcgctca cgacgacgtt
      481 catccccgga ggcaactcca acaggaacgt gctcacgggg ttcctcgcgg tggactcgga
      541 gacgggttcg gtggcgggtg cgccgagcgc cgactacggc aagctccggc tcctcgaatt
      601 gccgcgcgac acgacggtcc tcgggccggg ccagatgcag aacgccttca actcgaacac
      661 gtccgcccag aacgtcctga acctgctgcg ccagggcgag acggacgtga agagcgggaa
      721 cctcctcacc cttccggtgg gcggcgggct cctgtacgtc caacccgtct acgtccagag
      781 cagcaaggga acccagttcc cgctcctgag gaaggtcttc gtggccttcg gggactcggt
      841 gggcttcgcc gacacgctcg acgaggcgct cgaccaactc ttcggccgcc aggtgacgcc
      901 tccgaacccg ggaaccaccg atccagggac cacctctccc gggacgacct cgccgggcgg
      961 caccaccacc accggcccga cggctgaggc gctagccgcg ctacaccagg cgctcgcgga
     1021 cgcccaggcc gcgatcatcg acggacaggc cgcgttggcc accggtgact tcgcggcata
     1081 cggggaggct caggcacgcc tgcaggccgc gctccaggcc gccgttgacg ccgaggctat
     1141 cgtcaacggc gatggcgccg gaacgtcggc gacgtcggga gggacggtcg tggctccgtc
     1201 tccggggacc gcgggttagg acctaggagg aggcgttcgc ggcgcggacg gtgacgtcgc
     1261 ggccggtgac gtcgcgggcg gtgatggcgc ggagcgtcgc cgcgaggaat acccacgaga
     1321 cgaccaggag caccgcggag acaacccccg ccactccgag tggggagagg tcaatgcccg
     1381 cgagtggatc gttcgcgtcc ttgaacgcga cactgaagtc cgaggcggcg atgacctgag
     1441 ccgccccgac catcgacgcg acccccgagg cgatgtaggt cagccaccag gctcccacgg
     1501 tgcgtgatcg agccctgcgg ttgaggccgc ggaagtagag gtacggcatg acgatgctcg
     1561 cgagcggaat caaccagacg aaccacgccc agatctcgga gagcggctga gcctctcccg
     1621 cttccgtcag gcgccgatga accttgctca tccatagcgc gagggtgatg tacgagccga
     1681 gcatcagcaa ggaagcgagg cccgacagcc cctgcgaggc aaggtaggtc gcgtcgatgg
     1741 tcggattcga cccgacgtac tggtcgaagt tcgcatacat cgcgcgagag gccagcgcgc
     1801 cgaaaagggt cgcgacggcg acgccaccgg tcaggccgat ggtcgcggtc gtgagtcctg
     1861 tcggtcggcc agcggcccac ctggcgcggg gcgcgccgcc gaagggggga ggagtggggg
     1921 cggaggggat ggtcgtcatg agccgaatct agcgcgccga ggcccgatta tgcgtgccgc
     1981 cagcggtgac gtaggatggg gaatcgccga cgcggggtgg agcagctcgg tagctcgccg
     2041 ggctcataac ccggaggtcg caggttcaaa tcctgtcccc gctacgatga gggccctgac
     2101 catgtggtcg gggccctcca ctcgttttcc ccgaggtaac gcggcaaacc ggacacacct
     2161 tggtcgcgcg accgccctcc ggccgcagct tcgctagcgc gcgagccagg cgtcggccgc
     2221 gtcgaactcc tgctcgatgc ccctggccag cgcctcgccg accgcctgtg cgacggcggc
     2281 gctgatcagg aacgcgctcg acgacacgtt cccttcgacc gacatcctcg agccggagcc
     2341 gtccggcgcg agcgtcacgg atcccgcggc gcgggcaggg gctccgacga tctcgaccgc
     2401 gaacgtcgca tcgcgtgcct cgccctctgg ctccgtccat gcctccgtgt agatcaccga
     2461 gagcgtcgat ccgaggagcc cgcggaagtc cgaacgcacc ccctcggtcg cgatgacccg
     2521 tcggatgacg acgtcgaagg cgccgtcggc ggtgccgtca atcaacacgt cggaatcggt
     2581 cgagccggtc gcccgcgcgc gctcgcgcga gaactcctcg tcggcgtaca tggcgacgac
     2641 gtcggcaacc ggcgcacaaa accgatgcgc gaactcaacg tgcatgggac ccccttggcg
     2701 taccgaacca ctct
//
LOCUS       DASVKP010000174         7024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1045788, whole genome shotgun sequence.
ACCESSION   DASVKP010000174 DASVKP010000000
VERSION     DASVKP010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045788"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /gene="rpoA"
                     /locus_tag="VF362_03515"
     CDS             <1..680
                     /gene="rpoA"
                     /locus_tag="VF362_03515"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX7590028.1"
                     /translation="TGADIAPPAGVEIHNPELHIATLNSKAKFEVEMTVERGRGYVPA
                     TLNKQYDAEIGQIPVDSIYSPVLKVTYKVEATRVAQRTDFDELVIDVETKSSISPRDA
                     LASAGSTLVELFSLARGLNEAAEGIEIGPSDSDESLEEDYNLPVEELNLTQRSYNCLK
                     REGIHTVGELTARSEADLMDIRNFGQKSITEVKEKLTELGFSLKDSGGDYDPAVSGVY
                     FSGNEDN"
     gene            695..1285
                     /gene="rplQ"
                     /locus_tag="VF362_03520"
     CDS             695..1285
                     /gene="rplQ"
                     /locus_tag="VF362_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX7590029.1"
                     /translation="MPTPTKGARLGGGPAHERLMLANLSQSLFEHGRITTTLTRAKRL
                     RPYAERLITFAKRGDLASRRRVMGVIHDKSVVHTLFTEIGPSFAERNGGYTRITKVGI
                     RKGDSAPLAVIELVVGPLSDKQSVVAEASKAAKRAAKPAGSKDVESKDAAKKEAPAKE
                     APAKAAADRAVVEEATAEEPTADEAVAEADDAKAEA"
     gene            1311..2177
                     /gene="truA"
                     /locus_tag="VF362_03525"
     CDS             1311..2177
                     /gene="truA"
                     /locus_tag="VF362_03525"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX7590030.1"
                     /translation="MTDSAIVVRVRLDLSYDGTDFAGWADQPELRTVQGTLEAALKRV
                     VRAESLPRLVVAGRTDAGVHARGQVAHLDIPLAAWRRLPSRSRPGEAESLVARLNGVL
                     PSDIIVRAASEAPPGFDARFSASERRYAYRISDAGRSPDPLVRRHVLAYPRALDVGAL
                     TEASRSLTGLRDFCAFCRPRTGATTIRDLRELSWRREESGADAALVVATVRADAFCHS
                     MVRSLVGAVLAVGDGRRDAAWLRQVASSPHRSPAIAVAPGCGLTLEEVAYPTDAEVAL
                     RAAHTRGRRTLN"
     gene            2316..2759
                     /gene="rplM"
                     /locus_tag="VF362_03530"
     CDS             2316..2759
                     /gene="rplM"
                     /locus_tag="VF362_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX7590031.1"
                     /translation="MRTYSPKTGDHTAEWHVIDATDVVLGRLATHAAQLLRGKHKPSF
                     APHLDCGDFVIVINADKVALTGSKATTKMVYRHSGFPGGIKSIAIGDLLARDPRRVVE
                     NAVSGMLPHTRLGRAQMAKLKVYSGPEHPHSAQQPKAFPITQVAQ"
     gene            2789..3271
                     /gene="rpsI"
                     /locus_tag="VF362_03535"
     CDS             2789..3271
                     /gene="rpsI"
                     /locus_tag="VF362_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX7590032.1"
                     /translation="MEDETQDVQRETPTEYTSQSAEALVGTGKSAIAPGAGLGRRKEA
                     VARVRLVPGSGGWTINGRTLEDYFPNKVHQQLVNSPFALLNLESKFDVHARINGGGPS
                     GQAGALRLGVARVLNGIDVEANRGALKKAGYLTRDPRAVERKKAGLKKARRAPQYSKR
                     "
     gene            3282..4649
                     /gene="glmM"
                     /locus_tag="VF362_03540"
     CDS             3282..4649
                     /gene="glmM"
                     /locus_tag="VF362_03540"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX7590033.1"
                     /translation="MARLFGTDGVRGVANRDVTAELAVDLAVAAARVLGERGEFAGHR
                     PTAILGRDTRVSGQFLSAAVAAGLASAGVDVLDVGVVPTPAVAYLVSALEADLGVVIS
                     ASHNPMPDNGIKFFARGGQKLDDLVEASIEEKLGEAWNRPTGADVGTIQESSGLVRQY
                     VDHVVASAASFCGTDSPATGLVIVVDCAHGAAFGAGPAALRGAGATPIVLNAAPSGFN
                     INDGVGSTHLEPLRAAVLEAGADFGVAFDGDADRCLAVDHLGDVVDGDQIMGALAVAM
                     KGSRTLYRDTLVTTVMSNLGLHRAMRAAGIDVVEAAVGDRYVLEAMREGGYTLGGEQS
                     GHIIVSEYASTGDGTLTALLLAGIVATSGRSLRDLCSSVARLPQVLVNVSGVDRTRVF
                     TDPTLQEAVTAARAELGDAGRILLRPSGTEPLVRVMVEAETDETAREIAERLAGIVAA
                     RLGTA"
     gene            4646..5395
                     /locus_tag="VF362_03545"
     CDS             4646..5395
                     /locus_tag="VF362_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX7590034.1"
                     /translation="MRVLYGWTLSGFLASLQDQILSLTSSVWIYPSVWAVTVIDGVFP
                     PIPSESIVIAAATTWQQRGVPNVFALWLAAAFGAWCGDQIAYAIGMTIPVRKVPGMRG
                     PRGIAALDWAERALGHRGTSFIIAARFIPVGRVAVNLTAGALRFPRRRFMGIDAVAAA
                     IWATYSIGLGVFAGSLLHDNLLASIGIGVVGGVMLGYLVDLILSKIGVQPPAMVPVED
                     WPIDGPIALRRPPRTTKPEAPRGPKAPGGAS"
     gene            complement(5392..6348)
                     /gene="coaA"
                     /locus_tag="VF362_03550"
     CDS             complement(5392..6348)
                     /gene="coaA"
                     /locus_tag="VF362_03550"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEX7590035.1"
                     /translation="MDEAAIADAGSPFVTFSRAEWFALAGKTPLPLTEQDIARVRGLG
                     DPVDLSEVDHVYRPLSRLLDLYSTAIGALHDASNEFLGERASRTPFVIGVAGSVAVGK
                     STTARLLRELMARWPGSPRVELITTDGFLLPNAVLQERGILLKKGFPESYDRKALRAF
                     VAAVKSGLPTVTSPVYSHHTYDIVPGESITVARPDVLIVEGLNVLQPATLRDPEGDSL
                     TVSDYFDVSIYVDADAADVRRWYVDRFLSLRHTAFAQPDSYFRMFASLTDAEARERAG
                     EIWDSINAPNLERNIAPTRSRATIILRKGPDHAVERVYLRKL"
     gene            6428..>7024
                     /locus_tag="VF362_03555"
     CDS             6428..>7024
                     /locus_tag="VF362_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX7590036.1"
                     /translation="MCGIVGYVGSLEASSRPQEVVMAGLARLEYRGYDSAGIAVVTAD
                     RERLAMAKKAGKLAILAQELKDRPLPPGTVAIGHTRWATHGAPTDSNAHPHMAAGGRI
                     AIIHNGIIENYASLRSELEAEGVVFSSDTDTEVAGHMIAAEVDSGKGLAQAMRDVTRR
                     LEGAFTLLAVDAREPHTVVGARRNSPLVVGLGDGENFLG"
BASE COUNT         1139 a         2369 c         2405 g         1111 t
ORIGIN      
        1 tcacgggggc cgacatcgcg ccgcctgcgg gcgtcgagat ccacaacccc gaactccaca
       61 ttgccacgct caactccaag gcgaagttcg aggtcgagat gaccgtcgag cgcggacgcg
      121 ggtacgtccc ggccacgctc aacaagcagt acgacgcgga gatcggtcag attccggtcg
      181 actcgatcta ctcgccggtc ctcaaggtca cgtacaaggt tgaggcgact cgcgtcgcgc
      241 agcgtaccga cttcgatgaa ctcgtgattg acgtcgaaac caagtcgtcc atctcgcccc
      301 gcgacgcgct cgcgtcggcc gggtcgacgc tcgtggaact cttctcgctc gctcgcggcc
      361 tcaatgaggc tgcggagggc atcgagatcg gaccctcgga cagcgacgag tcgctcgaag
      421 aggactacaa cctgccggtc gaggaactca acctcaccca gcgctcgtac aactgcctca
      481 agcgcgaagg catccacacc gtgggtgaac tcacggctcg tagcgaggcc gacctcatgg
      541 acattcgcaa cttcgggcag aagtcgatta ctgaggtcaa ggagaagctg accgagctcg
      601 gcttctcgct caaggacagc ggcggcgact acgaccccgc ggtctcgggc gtctacttct
      661 cgggtaacga agacaactag agggagttca gatcatgcct accccgacca agggtgcccg
      721 gctcggcggc ggaccggctc acgagcgtct catgctcgcg aacctctccc agagcctgtt
      781 cgagcacggc cggatcacga cgacactgac gcgcgccaag cggcttcggc cgtacgccga
      841 gcggcttatc accttcgcga agcgcggtga tctcgcctct cgccggcgcg tcatgggcgt
      901 catccacgac aagtccgtgg tgcataccct cttcaccgag atcggcccgt cgttcgcgga
      961 gcgaaacggc ggatacacgc gcatcaccaa ggtgggcatc cgcaagggtg acagcgcgcc
     1021 gctcgccgtt atcgaactcg ttgttggccc gttgagcgac aagcagtccg tcgtcgccga
     1081 ggcctccaag gccgcgaaga gggctgcgaa gcctgcgggt tcgaaggacg tcgagtccaa
     1141 ggacgccgcc aagaaggagg cccccgcgaa ggaggccccc gcgaaggcgg cggccgaccg
     1201 ggccgtcgtg gaggaggcga ccgcggaaga gccgaccgct gacgaagcag tcgccgaggc
     1261 ggacgacgcc aaggccgaag cgtagacacg acctcatcga ggcggatccc gtgaccgaca
     1321 gcgccatcgt cgtgcgggtc cgcctcgatt tgtcgtatga cgggacggac ttcgcgggat
     1381 gggccgatca acccgagctg aggaccgtcc agggcactct cgaggccgcc ctcaagcggg
     1441 tcgtccgtgc ggagagcctt ccccgccttg tcgtcgcagg tcggacggat gcgggcgtcc
     1501 acgcgcgtgg ccaggtggca cacctcgaca tccccctcgc tgcgtggagg cggcttccga
     1561 gtcggtcccg gccgggggag gcggagtcgc tcgtggctcg cctcaacggc gtgcttccgt
     1621 cggacatcat tgtgcgcgca gcgtcggagg cgccccccgg attcgacgcc cgattcagcg
     1681 cctccgagcg gcgctatgca taccgcatct cggacgcggg acggtccccc gatcccctcg
     1741 tcaggcgcca cgtccttgcc tacccgcgcg cgctcgacgt cggcgcgctc accgaggcct
     1801 ctcgatcgct caccggcctc agggacttct gcgcgttctg tcggccccga actggcgcga
     1861 cgaccatccg cgatctcagg gaactgtcgt ggcgacgcga agagtccggg gcggacgccg
     1921 cgctcgtcgt cgccaccgtt cgcgccgacg ccttctgcca ttccatggtt cgctcgctgg
     1981 taggcgcggt gctcgccgtc ggagacgggc gccgcgatgc cgcgtggctt cgccaggtcg
     2041 cctcgtcacc acaccgatcg cccgcgatcg cggtggcgcc cggttgcggc ctgacccttg
     2101 aggaagtcgc gtaccccacc gacgcggaag tggcgcttcg cgccgcccac acccgtggca
     2161 ggcggaccct gaattgaccc accccaacct ggacggtatg ctcaagggtc gttgtgcgtt
     2221 ccaccgcggg ctggttgaat cctccgctat cggaatggcc gaccgccacg ctggatagac
     2281 cagcgaccat cgatgagcaa cagagaaggc ttcacgtgcg cacctactcc cccaagaccg
     2341 gtgaccacac tgccgagtgg catgtgatcg atgccaccga cgtggtgctg ggtcgtctcg
     2401 cgacccacgc ggcccagttg ctgcgcggca agcacaagcc ctcgtttgcc ccgcacctgg
     2461 attgcggcga tttcgtgatc gtcatcaacg ccgacaaggt ggccctgacg ggctccaagg
     2521 cgacgacgaa gatggtctac cgccactcgg gcttcccggg cggaatcaag tcgatcgcga
     2581 tcggtgacct tctcgctcgc gacccccgtc gcgtcgtgga gaacgcggtg agcggcatgc
     2641 tcccgcacac ccgtcttggg cgcgcccaga tggccaagct caaggtgtac tcgggtcccg
     2701 agcacccgca ctcagcgcag cagcccaagg ctttcccgat cacccaggtc gcgcagtagc
     2761 gcgcgggtca ggacaaagga caaacaccgt ggaagacgag acgcaggacg tccaacgtga
     2821 gacgcccacc gagtacacct cgcagagtgc tgaggcgctc gtgggcaccg gcaagtcggc
     2881 gatcgctccc ggcgccgggc tcggtcgacg caaggaggca gtcgcccgcg tgcggctcgt
     2941 ccccggatcc ggcggatgga ctatcaacgg ccggacgctc gaggactact ttccgaacaa
     3001 ggttcaccag caactcgtga acagcccgtt cgccctcctg aacctcgagt ccaagttcga
     3061 cgtccacgcc cgcatcaacg gtgggggacc gtctggccag gccggggcct tgaggctcgg
     3121 cgtcgcgcgg gtcctgaacg gcattgacgt tgaggcgaac cgcggcgcgc tgaagaaggc
     3181 cgggtacctc acccgcgacc cgcgcgccgt cgaacgcaag aaggcgggcc tcaagaaggc
     3241 gcgccgcgcc ccgcagtact cgaagcgata gcatttcgcg catggcacgc ctgttcggta
     3301 ccgacggtgt gcgtggagta gcgaaccggg atgtgacggc cgaattggcc gttgacctcg
     3361 cggtcgcggc agcccgcgtc ttgggcgagc gaggtgaatt cgcgggccat cgccccacgg
     3421 cgatccttgg ccgcgatacc cgcgtgtccg gccagttcct gtccgctgcg gttgcggcgg
     3481 gcctcgccag cgcgggcgtc gacgtcctcg atgttggcgt cgtccccacc ccggcggtcg
     3541 cctatctggt gagcgcgttg gaggccgacc tcggggtcgt gatctccgct tcccacaacc
     3601 ccatgcccga caacggcatc aagttcttcg cgcgcggcgg gcagaagctg gacgacctcg
     3661 tcgaggcgtc gatcgaggag aagctcgggg aggcgtggaa ccggcctacc ggggccgacg
     3721 tggggacgat ccaagaatcc agcggactcg tacgccagta tgtcgatcac gtcgtggcgt
     3781 ccgccgcgtc tttctgcggg acggatagcc cggcgacggg attggtgatc gtggtcgatt
     3841 gcgcgcacgg tgccgcgttc ggcgcgggac ccgcggccct ccgcggagcg ggtgcgacgc
     3901 cgatcgtcct caatgcggcg ccaagtggtt tcaacatcaa cgacggcgtc ggttcgacgc
     3961 acctggaacc gctgcgcgcg gcggtgctcg aggcgggagc cgacttcggc gttgccttcg
     4021 atggggacgc ggaccgatgc ctcgccgttg accacctcgg ggacgtcgtg gatggcgatc
     4081 agatcatggg agccctcgcc gtcgcgatga agggctcgcg gacgctctac cgcgacaccc
     4141 tcgtgacgac cgtgatgagc aacctcggcc tccaccgcgc catgcgagcc gccgggatcg
     4201 atgtcgtgga ggcggcggtg ggtgatcggt acgtcctcga ggcgatgcgc gagggcggct
     4261 acaccctcgg gggcgagcag tcggggcaca tcatcgtctc cgagtacgcg tccacgggcg
     4321 acgggacgct caccgcgctc cttctcgccg gcatcgtcgc gacctcgggc cggagcctga
     4381 gggacctgtg ctccagcgtg gcgcgcctgc ctcaggtcct agtcaacgtg tcgggtgttg
     4441 accgcaccag ggtattcacg gaccccaccc tccaggaggc agtgaccgca gcgcgcgccg
     4501 aacttggcga cgccggacgg atcctcctgc ggccgtcggg gaccgagcca ctcgtgcgcg
     4561 tgatggtcga ggccgagacc gacgagaccg cgagagagat cgccgaacgc ctcgctggca
     4621 tcgtcgcagc gaggctcgga acggcgtgag ggttctttac ggctggaccc tgtctggctt
     4681 cctcgcgagc ctgcaggacc agatcctgtc gctcacctcg tccgtgtgga tctacccctc
     4741 ggtgtgggcc gtcacggtga tcgacggcgt attcccgccg atcccttccg agtccatcgt
     4801 catcgccgcg gcaaccacgt ggcaacagag gggcgtcccc aacgtctttg ccctctggct
     4861 cgcggcggcg ttcggggcgt ggtgcggcga tcagatcgcc tacgcgattg ggatgacgat
     4921 ccccgtgcgg aaggttcccg gcatgcgggg cccgcgtggg atcgccgcgc tcgattgggc
     4981 cgagcgggcg cttgggcacc gcgggacctc cttcatcatc gcggcgcggt tcattccggt
     5041 cggtcgcgtg gccgtcaatc tcaccgctgg ggccttgagg ttcccccgga ggcgcttcat
     5101 ggggatcgac gcggtcgctg cggcgatctg ggcgacgtat tcgatcggcc tgggggtgtt
     5161 cgcgggctcg ctactgcatg acaacctttt ggcgagcatc gggatcgggg tagtcggcgg
     5221 ggtcatgctg ggctacctcg tcgacctcat cctgtcgaag atcggggtcc agccgcccgc
     5281 gatggtcccc gtagaggatt ggccgatcga cgggccgatc gcgctccgcc gcccgccccg
     5341 cacgactaag cccgaagcgc cgagaggccc caaggccccg ggcggggcca gctagagctt
     5401 gcgcaggtag acgcgctcca cggcgtggtc cgggcctttg cgaaggatga tcgtcgcgcg
     5461 tgaccgggta ggggcgatgt tgcgctcgag gttcggggcg ttgatggagt cccagatctc
     5521 gcctgctcgc tccctcgcct ccgcatcggt cagcgacgcg aacatacgga agtacgagtc
     5581 cggctgagcg aacgcggtgt ggcgcaggga caggaagcga tctacgtacc accgccggac
     5641 gtcggcggcg tccgcgtcaa cgtagatcga gacgtcgaag tagtcgctta cggtgagcga
     5701 atcgccctcg ggatcccgca gcgtcgccgg ctggaggacg ttgagtcctt cgacgatcag
     5761 cacgtcggga cgtgccacgg tgatcgactc tcctgggacg atgtcgtagg tgtggtgcga
     5821 gtagacgggc gaggtcacgg tcggaagccc cgatttcacc gcggcgacga aggcgcgcag
     5881 cgcctttctg tcgtaggact cggggaatcc cttcttgagc aggatgccgc gctcctgcag
     5941 gaccgcgttc ggaagcaaga atccgtcggt ggtgatgagc tcaacccgcg ggctcccggg
     6001 ccaccgcgcc atgagttccc gcaacaggcg cgccgtcgtc gacttgccga cggccaccga
     6061 gccagcgacg ccgatgacga acggggtgcg cgaggcgcgc tcgccgagga actcgttcga
     6121 ggcgtcatgg agagcgccga tcgccgtcga gtagagatcg agtagccgtg agagcggacg
     6181 gtagacgtgg tccacctcgg acagatcgac cgggtcgccg aggcccctga cccgcgcgat
     6241 gtcctgctcc gtgagcggca agggggtctt ccccgcgagc gcaaaccact cggcgcggct
     6301 gaacgtgacg aacgggcttc ccgcgtcagc gatggcggcc tcgtccatgc ccccattgtg
     6361 gcccatccgt ggtcgccgag ggcgccgcgg ggcgccgcac ctgatcccgg caactagact
     6421 cgtcgtcatg tgcggcatcg tgggatacgt cggttcgttg gaggcgagtt cacggccgca
     6481 ggaggtggtc atggcgggcc ttgcgaggct cgagtatcgc gggtacgact ccgcgggcat
     6541 cgcggtggtg accgccgaca gggagcgcct ggcgatggcc aagaaggcgg gcaagctcgc
     6601 gattctcgcc caggagctga aggacaggcc gctcccgccc ggcaccgtcg cgatcgggca
     6661 cacccggtgg gctacccacg gcgccccgac cgattccaat gcgcaccccc acatggccgc
     6721 gggcgggcgt atcgcgatca ttcacaatgg gatcatcgag aactacgcga gccttcgttc
     6781 ggagctcgag gcggagggcg tggttttctc gtccgacacc gacaccgagg tcgcgggaca
     6841 catgatcgcg gccgaggttg acagcggcaa gggccttgcc caggcgatgc gtgacgtgac
     6901 ccgtcgcctg gagggtgcct tcacgcttct cgccgtcgac gcgcgggagc cgcacaccgt
     6961 cgtgggcgcg cgccggaact cgcccctcgt cgtggggctc ggggatgggg agaacttcct
     7021 cggc
//
LOCUS       DASVKP010000175         5894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1128249, whole genome shotgun sequence.
ACCESSION   DASVKP010000175 DASVKP010000000
VERSION     DASVKP010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128249"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VF362_03560"
     CDS             <1..1054
                     /locus_tag="VF362_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590037.1"
                     /translation="TTLATVGDLWRLESVTTFRDDAAPGPTVVYLVSPEGDRYLAGTV
                     PTPELTVLAWDTAGDAALVGGQTTLFRIDLATGAVTPDDPPATLTYGGATRMGVGTDG
                     RTYFNLGNEPVDGAGHTGVWAWSAGGWEGVALPLPSDPYGYETTDAIVPGGEWLLWGE
                     DTHSSPRTDHLFNLSTGVDSPLASIGDGCSADGWHDATRLDAYCWDTPDASGRHRLFD
                     VTSGTFTDAPDSSNPTDGYVYSSAQLPGTPLVFRFDDTPGFVSSIGVLTKDGLKTITT
                     VAPEDQFQIGDVIEVRPGTYVIRDGSGTTPGDEVVRAVIDAVSAFAAFGTASGPGDPS
                     PHRALRTTILVNYEGA"
     gene            complement(1074..1757)
                     /gene="trmD"
                     /locus_tag="VF362_03565"
     CDS             complement(1074..1757)
                     /gene="trmD"
                     /locus_tag="VF362_03565"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX7590038.1"
                     /translation="MLRIDLVTIFPEYFSVLGVSLLGKARAAELVETHVHDLRSWTTD
                     VHHAVDDSPFGGGAGMVMKADVWGLALDDVLAEGGRLIIPSPAGVPFTQDMAEELARE
                     EQLVFACGRYEGIDARVAEHFAARHRVSEISLGDYVLNGGEVAALAIIEAVARLLPGF
                     VGNAESLVEESHADGRLEYPSYTRPASWRGHEVPEVLLSGNHAAIAAWRREEAERLTR
                     LRRPDLLGD"
     gene            complement(1744..2265)
                     /gene="rimM"
                     /locus_tag="VF362_03570"
     CDS             complement(1744..2265)
                     /gene="rimM"
                     /locus_tag="VF362_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012877942.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX7590039.1"
                     /translation="MDLVIARIGRAHGLGGEVSVELRTDIPEERFVVGAVLQTDPTGA
                     GPLTLTSARHDSGPWYLRFAEATSREAAEGLRGVLLVVEQDESDEEDAWYPHELIGLR
                     AERPTGEVVGTVSGIEHPPAHDLLVIREPGGTTARVPFIAAMVPVVDVAGGRVVVDPP
                     FGLLAGEDADATD"
     gene            complement(2297..2536)
                     /locus_tag="VF362_03575"
     CDS             complement(2297..2536)
                     /locus_tag="VF362_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX7590040.1"
                     /translation="MILDAVDFLVKSIVRNPDDVRVTKRPGRRGPQLNVVVHPDDLPR
                     VIGRHGRTAEAIRTVIDAIAREDVHVNFVDVAPRS"
     gene            complement(2699..3169)
                     /gene="rpsP"
                     /locus_tag="VF362_03580"
                     /pseudo
     CDS             complement(2699..3169)
                     /gene="rpsP"
                     /locus_tag="VF362_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            complement(3311..3925)
                     /locus_tag="VF362_03585"
     CDS             complement(3311..3925)
                     /locus_tag="VF362_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590041.1"
                     /translation="MNVRKTVLIGVVGASALMGASCGGSPATSTATVTGPSESGGPSA
                     AATATTTTTTTTTTTAAHVWVFGDEWTHTSASGAQGSGCAPGAGKFPDGAWFGFAKSW
                     STSQIAFDLACWYSGAAGEAQAAAHGTEFTNDYFVTNDNTTVRLVTVAGDIPAKKAAW
                     DDGVFKLSQVMVDPGGTLPTSALYPAWIFVNGGVVTELEVQYVP"
     gene            complement(3993..4529)
                     /locus_tag="VF362_03590"
     CDS             complement(3993..4529)
                     /locus_tag="VF362_03590"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEX7590042.1"
                     /translation="MKGSRIVFVAGASAVMLAAGWRAGSLPVSTLALANPASGTVSTG
                     PSPSPTPTATTTAPTTTKTTKTTKTTKTTTTTAPPPAPAPAGATGTYVGPSVSTAYGN
                     MQVQLVVTNGKITKVQPLVIGYGDRTSQRINSSAVPTLQQRVLKAQTYKVSYVSGASY
                     TSQGFLASVKGAMAKARL"
     gene            complement(4540..>5894)
                     /locus_tag="VF362_03595"
     CDS             complement(4540..>5894)
                     /locus_tag="VF362_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HEX7590043.1"
                     /translation="AEHAERGRRARQLSLWRDSLLGAAWLVTVAGVAFFFASGAMAWT
                     APVDAVNALGRFAGLVATALILFQMALASRAPWIERAVGHDKALKIHGSLGEPVFYLL
                     VAHAALLVVGQGSLASRGALDQTAWYLAHSRDIALSILSLVLLLIVGVTSVAAVRRRW
                     PYETWHAIHLFTYAAIALAVPHQFGVGSTFSSSTPAKVFWIALYVIAVGSLVIWRMWM
                     PLYRAARHRLRVMRVERHDDGTVSVTVGGRNLHYWTAHAGQFFIWRFLTPELWATAHP
                     YSLSAAPDGRSLRITVKPLGDDSLALRRLRPGVRVIAEGPLGRFHHDSRVGRGLVLIG
                     AGVGITPLRAMLEDECARRGECTVILRASTHLEAPLLDEIRQLAVTQGATLYEVIGPR
                     GKGWSTPDGPQSLSQIIPRLSTCDVFVCGPEKWSARVKADARHHGVPEQAIHAEEFAW
                     "
BASE COUNT          886 a         2059 c         2059 g          890 t
ORIGIN      
        1 gacgactctc gctacggtcg gggacctgtg gcgcctggaa tcggtcacga ccttccgcga
       61 tgacgccgcc ccaggcccca ccgtcgtcta tctagtctcg cccgaaggtg accgctacct
      121 ggccgggacg gtgccgacgc cggaactgac ggtgctggca tgggacaccg caggcgacgc
      181 ggccctcgtc ggcggccaga ccaccctctt ccgaatcgac ctcgcgaccg gtgcggtcac
      241 gccggacgat ccgcccgcga cattgacgta cggcggcgcg accagaatgg gggtcgggac
      301 cgacggccgc acctacttca acctgggcaa cgaacccgtc gacggggccg gtcacaccgg
      361 cgtctgggca tggtccgcgg gcggttggga gggcgtcgcc ctccctcttc cctccgatcc
      421 gtacggctac gagacgaccg acgccatcgt ccccggaggc gaatggctgc tctggggtga
      481 ggacacgcat tcctctccac gaacggatca cctgttcaac ctcagcacgg gagtcgattc
      541 gcccctcgct tcgatcggcg acggctgctc ggcggacggc tggcacgacg caacgcgcct
      601 cgacgcctac tgttgggaca ccccggacgc ttccggtcgt caccggctct ttgacgtgac
      661 gtcgggcacg ttcacggacg cgcccgacag ttcgaacccg accgatggct acgtctactc
      721 gtcggcgcag ttgcccggaa cgccgctcgt cttccgcttc gacgacacgc ccggcttcgt
      781 ctcgtcaatc ggcgttctca ccaaggacgg gctgaagacc atcaccaccg tcgcgcccga
      841 ggaccagttc cagatcggcg acgtcatcga ggtgcgcccc ggcacctacg tgattcggga
      901 cggctccggc acgaccccgg gcgacgaggt ggtccgcgcg gtcatcgacg cggtttccgc
      961 cttcgccgcc ttcggcacgg cgagcggccc gggggatccc tccccccatc gcgcactgcg
     1021 gacgaccatc ctcgtcaact acgagggcgc gtaggcgcag ggacggcgcg agcctagtcc
     1081 ccgagcaggt ccgggcgcct gaggcgcgtg agccgctccg cctcctcgcg gcgccaggcc
     1141 gcgatcgccg cgtggttgcc gctcagcagc acctcgggca cctcgtggcc gcgccacgac
     1201 gcgggccggg tgtagctcgg atactccagc cgaccgtcgg cgtggctctc ctcgacgagg
     1261 ctctcggcgt tcccgacgaa gccgggaagg agccgcgcga cggcctcaat gatcgcgagg
     1321 gcggccacct ctcccccgtt gagcacgtag tcgccgagcg aaatctcgct gacgcggtgc
     1381 cgcgccgcga agtgctccgc gacccgcgcg tcgatgccct cgtagcgccc gcacgcgaac
     1441 acgagttgct cctcgcgcgc cagctcctcg gccatgtcct gggtgaacgg cacgccagcg
     1501 ggcgagggga tgatgaggcg cccgccctcg gcgagcacgt cgtcgagcgc gaggccccag
     1561 acgtcggcct tcatcaccat gcccgcgccc ccgccgaagg gcgagtcgtc gacggcgtgg
     1621 tgcacgtccg tggtccagga gcgcaggtcg tggacgtggg tctcgacgag ctcggccgcg
     1681 cgggccttgc caaggagcga gacgcccagg acggagaagt actcagggaa gatcgtgacg
     1741 aggtcaatcc gtagcatcgg cgtcctcccc cgcgagcagg ccgaagggcg ggtcgacgac
     1801 gacgcggccg cccgcgacgt ccacgacggg aaccatcgcc gcgatgaacg ggacgcgcgc
     1861 cgtggtgccg ccgggctcgc ggatcacgag caggtcgtgg gcgggcgggt gctcgattcc
     1921 ggagacggtg ccgacgacct cgccggtcgg gcgctcggcc ctgagcccga tgagttcatg
     1981 ggggtaccag gcgtcttcct cgtccgactc gtcctgctcg accacgagca gcacgcccct
     2041 aagcccctcg gcggcctcgc ggctcgtcgc ctccgcaaat cgcaggtacc aggggccgga
     2101 gtcgtgccgg gccgaggtga gcgtgagcgg gccagcgccc gtcggatcgg tctgaaggac
     2161 cgccccgacg acgaagcgct cctcggggat gtcggtgcgg agctcgacgg acacctctcc
     2221 gccgagcccg tgcgcgcgcc cgatgcgggc gatcaccagg tccatcgaaa gccccttccg
     2281 cgcgggaggg acgacgctac gagcgcgggg cgacgtccac gaagttcacg tggacgtcct
     2341 cgcgcgcgat cgcgtcgatg acggtgcgga tcgcctcggc cgtgcggccg tggcgtccga
     2401 tgacgcgcgg caggtcgtcg ggatggacca ccacgttgag ttgcggtccg cgcctgccgg
     2461 gacgcttggt gacgcgaacg tcgtccgggt tacggacgat cgacttgacc aggaagtcca
     2521 ccgcgtcgag gatcacggtc tacttctcct cgtcgttgat gccttcgacc ttgtcgtcgg
     2581 cgtcggacgg gtcgccctcg gcgacctcga tctcgacctc ggcgccctca tcagagggaa
     2641 cctcgtgcga ctcgtcggcg atggggtctt cgacgaccgc ggcggcggcc tccgtagctg
     2701 cggactcagc tgcagctgct gctgcttctg cggccgcttc agcggcggcc gtcgcggcct
     2761 tggcgtcttc ctcggccttt gccttcactg cgtcctccgt tgccttggcc ttggtggcct
     2821 tcgccttctc ggcggcgccc gccgcggcct tgatcctctc gtcgggacca accttgacga
     2881 tctggggctt gacggtgttc gtcgcgccct tctctcccgt gaagatgccc cagtcgccgg
     2941 tgagcttcaa gagcgcggcg acctgcgggg tgggctgggc accgacgccg agccagtact
     3001 gcacgcgctc ggagttgatg acgatgaccg agggctcctc ggtggggtgg tacttcccga
     3061 tctcctcgat ggcgcggccg tcacgacggg agcgcgagtc catgacgacg acgcggtaga
     3121 agggggcgtg cttcttgccg aagcgcttga ggcggatctt gacagccacg agtatctcct
     3181 gggattcggt gtgagtcagt cccggctacc cgcggagcgg gggcggtccg acaagacgaa
     3241 tagcgacgcg gaggaaaccg ggttcgccag gtgcaagtgt gccagacgta cgggggtccg
     3301 gcaaacgccc ctagggcacg tactggactt cgagttccgt gacgacgccg ccattgacga
     3361 agatccatgc ggggtagagg gcgctcgtgg gcagggtccc gccgggatcg accatcacct
     3421 gggacagctt gaagaccccg tcgtcccacg cggccttctt cgcgggaatg tcccccgcga
     3481 ccgtaacgag cctgaccgtc gtgttgtcat tcgtgacgaa gtagtcgttg gtgaactcgg
     3541 tcccgtgagc cgcggcctgc gcctcccccg ccgcgccgga gtaccagcac gcgaggtcga
     3601 aggcgatctg cgacgtcgac caactcttcg cgaacccgaa ccacgcccca tcgggaaact
     3661 tgcccgcccc aggggcgcat ccggagccct gcgccccgct cgccgacgtg tgcgtccact
     3721 cgtccccaaa cacccacacg tgggctgctg tcgttgtggt ggtcgtcgtg gtggtggtcg
     3781 tcgcggtcgc agccgccgag gggccgcccg actccgacgg tcccgtcacc gtcgcggtcg
     3841 acgtcgccgg cgaacctccg cacgacgcgc ccatcagcgc gctcgcaccc acaaccccga
     3901 tgagcaccgt cttgcgaacg ttcatgattc ctcccaccag aaacgctagc accggcgacg
     3961 gcgcgctccc aagggccgga tcggagggcc agctagagcc gggccttcgc catcgcaccc
     4021 ttgacggatg ccaggaagcc ctggctcgtg tacgaggcgc cggagacgta cgagaccttg
     4081 taggtctgag ccttgaggac gcgctgctgc agggtcggaa cggcgctcga gttgatcctc
     4141 tgcgatgtcc ggtcgccata gccgatgacg agcggctgaa ccttggtgat cttcccgttg
     4201 gtgaccacca gttggacctg catgtttccg taggccgtgc tcacggacgg accgacgtag
     4261 gtgcccgtcg cgccggccgg cgcgggcgcg ggcggcggag ccgtcgtcgt ggtggtcttc
     4321 gtggtcttcg tggtcttcgt ggtcttcgtg gtcgtcggcg ccgttgtcgt tgccgtcggc
     4381 gtcggcgagg ggctcgggcc cgtcgagacg gtgccgcttg ccgggttggc gagcgcgagc
     4441 gtcgaaacgg ggaggctgcc tgccctccac cccgccgcga gcatcaccgc ggatgctccc
     4501 gcaacgaaga ctattcgtga acccttcacc gctgacctcc taccacgcga attcctcggc
     4561 gtggatcgcc tgttcgggga cgccgtggtg acgggcatcc gccttgactc gcgcggacca
     4621 cttctcgggc ccgcacacga acacatcgca cgtcgacagc ctggggatga tctgagacag
     4681 cgactggggg ccgtcggggg tcgaccaacc cttgcctcgc ggcccgatga cctcgtacag
     4741 cgtggcgccc tgcgtgaccg cgagctgccg gatctcgtcc agcaagggcg cctcaaggtg
     4801 cgtgctcgcc cgcaggatca ccgtgcactc gccgcgccga gcgcactcgt cctccagcat
     4861 cgcccgaagg ggggtgatgc cgacgcccgc gccgatcagg acgagcccac gccccacccg
     4921 ggagtcgtgg tggaagcgcc cgaggggccc ctccgcgatc acccgaacgc ccggccgtag
     4981 cctccgcagc gccagggagt cgtcgccgag cggcttgacc gtgatccgaa gcgaacgccc
     5041 gtccggggcg gcggagagcg agtaggggtg cgccgtcgcc cacaactcgg gcgtgaggaa
     5101 cctccagata aagaactgcc cggcgtgcgc agtccagtag tggaggttgc gcccgccaac
     5161 cgtcacggag acggtgccgt catcgtggcg ctcgacgcgc atcactcgga ggcggtggcg
     5221 tgcggcgcgg tagaggggca tccacatgcg ccagatcaca agcgagccaa cggcgatgac
     5281 gtagagggcg atccagaaga ccttcgcggg cgtgctcgag gagaaggtcg agccgacgcc
     5341 gaactggtgg ggcaccgcga gcgcgatggc cgcgtacgtg aagaggtgga tcgcgtgcca
     5401 ggtctcgtag ggccagcgcc gccggacggc ggcgaccgac gtcaccccga cgatgaggag
     5461 tagcacgagc gagaggatgg agagcgcgat gtcgcggctg tgcgccaggt accatgccgt
     5521 ctggtcgagc gcgccccggc tggcgagcga gccctggccg acgacgagca gggccgcgtg
     5581 ggcgacgagg aggtagaaga ccggctcgcc gagcgatccg tggatcttga gcgccttgtc
     5641 gtggccgacg gcccgctcga tccacggcgc gcgcgacgcg agcgccatct ggaagagaat
     5701 gagcgccgtc gcgacgagcc cggcgaaccg gccgagggcg ttgaccgcgt cgacgggggc
     5761 cgtccacgcc atcgcgccgc tcgcgaagaa gaaggcgacc ccggccacgg tgacaagcca
     5821 ggcggcaccc agcagggagt cgcgccacag ggagagctgg cgggcgcggc gcccgcgttc
     5881 ggcgtgctcg gcga
//
LOCUS       DASVKP010000176         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_386218, whole genome shotgun sequence.
ACCESSION   DASVKP010000176 DASVKP010000000
VERSION     DASVKP010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386218"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VF362_03600"
     CDS             <1..183
                     /locus_tag="VF362_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017199328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7590044.1"
                     /translation="KVAVKEGAVVEEGDLIAVIEAMKMEQPLVAHRPGTVRNLAAEVG
                     AGVAAGATICEIESVE"
     gene            300..1187
                     /gene="efeU"
                     /locus_tag="VF362_03605"
     CDS             300..1187
                     /gene="efeU"
                     /locus_tag="VF362_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HEX7590045.1"
                     /translation="MFANFLIGLREGLEAALVVGILVAYLIRSDRRELLPHVWIGVAI
                     ATTASLGAGAALTFGPRGLSARAEEAIAGTLSVLAVGLITWMIFWMARTARDLRRHLE
                     TSLDAAVSMGKGAIVAMALLAVGREGLETAVFLWAGIDTAGAGAGPIAGAVLGLACAL
                     ALGRIIYRGALRVDLRAFFRWTGLVLIAVAAGVLAHAVHDLQDAGVLPGAHALAFDVS
                     AQIPPDSWHGSLLGGAFNFSPATTWLEALTWAAYAIPTLVGFARISFARRPTSAPEAL
                     RAAGSQGVRAEFAATTSNA"
     gene            1284..>1735
                     /locus_tag="VF362_03610"
     CDS             1284..>1735
                     /locus_tag="VF362_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590046.1"
                     /translation="MTGPQRRATGLVAVSVWLTLDLVRFSGPLISQLFDVGVGIAAAA
                     ATAAFVGGGIVAFVGFLVARQIGHGRVLMWIAGALVVLRVLLPFLTEDALITYGLIAL
                     ALALGLVLLAAAGTADVAGGAGVLAAAGTGAMVALLEQGILRTWDALWR"
BASE COUNT          212 a          619 c          618 g          286 t
ORIGIN      
        1 aaggtcgcgg tgaaggaggg cgccgtcgtc gaggagggcg acctcatcgc ggtcatcgag
       61 gccatgaaga tggagcagcc gctcgtcgct catcgacccg gaacggtcag gaacctcgcg
      121 gccgaggtcg gggcgggagt cgccgcgggc gcgacgatct gcgagatcga gtccgtcgag
      181 taggaccctg cgaattacgc aacacagtcg ttgcgtttat ttgacaaccc gtgccgtccc
      241 ccctacgctg gagcccacag gttagggtac cctaacccct ggccccgaag ggtgacgccg
      301 tgttcgcgaa cttcctgatc ggcctgcgcg aggggctcga ggcggcgctc gtcgtcggca
      361 tcctggtcgc ctacctgatc aggagcgatc gccgggagct cctgccgcac gtgtggatcg
      421 gcgtcgcgat cgcgacgacc gcctcgctcg gggcgggcgc ggccctcacg ttcggccccc
      481 gcggactgtc ggcccgggcg gaagaggcga tcgcgggcac cctcagcgtc ctcgcggtgg
      541 gcctcatcac ctggatgatc ttctggatgg cgcggaccgc gcgcgatctc cgtcggcacc
      601 tcgagacgtc gctcgacgcc gccgtctcga tgggcaaggg cgcgatcgtc gcgatggcac
      661 tcctcgcggt cgggcgcgaa gggctcgaga cggcggtctt cctgtgggcg ggcatcgaca
      721 ctgcgggcgc gggcgccggc cccatcgcgg gagccgtcct ggggctggcc tgcgccctgg
      781 cgctcggccg gatcatctac aggggggcgc tacgcgtcga ccttcgcgcc ttcttccggt
      841 ggaccggcct cgttctcatc gccgtcgcgg ccggggtcct cgcccacgcg gttcacgacc
      901 tccaggacgc cggggtcctc cccggggcac acgccctcgc cttcgacgtc tccgcccaga
      961 tcccgccgga ctcgtggcac gggagcctcc tcgggggcgc cttcaacttc tcgcccgcga
     1021 cgacctggct cgaggccctc acctgggccg cgtacgccat cccgacgctc gtggggttcg
     1081 cgaggatctc cttcgcccga cgcccgacgt cggccccgga ggcgcttcga gccgcgggca
     1141 gccagggcgt gcgcgcagag ttcgcggcga ccacgtcgaa cgcctagagc gggttcgcgg
     1201 cacccgagtc gcgcggcagg ggggcgtccg acgcggccga acccgtcgtg tcgcgatccg
     1261 cgcgatacgg atatggtgac tccatgactg gacctcagcg acgagcaacg ggccttgtag
     1321 cggtctccgt gtggctcacc ctcgaccttg tccgattcag cggacccctc atcagccagc
     1381 tcttcgatgt cggtgttggc atcgccgcgg cggccgcgac cgcggcattc gtcgggggcg
     1441 gcatcgtggc attcgtcggg ttcctcgtcg cgcgccagat cggacatggg cgggtgctga
     1501 tgtggatcgc tggcgctctc gtcgtcctgc gagtcctcct gcccttcctt accgaggacg
     1561 cgctcatcac ctacgggctg attgccctcg cgctcgcgct cggcctggtt ctcttggcgg
     1621 cggcaggcac cgccgacgtc gccggggggg ctggggtgct ggcggccgcg ggcacgggcg
     1681 ccatggtcgc cctgctcgag cagggcatcc tccggacctg ggacgccctg tggcg
//
LOCUS       DASVKP010000177         1808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_386438, whole genome shotgun sequence.
ACCESSION   DASVKP010000177 DASVKP010000000
VERSION     DASVKP010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386438"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VF362_03615"
     CDS             <1..705
                     /locus_tag="VF362_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEX7590047.1"
                     /translation="GVGSGALTMSLLRAVGDGGSLLSVERREDFAAIARANVEAVFGG
                     PHPAWQLEVGDFADVATARCEPGSVDRVVLDMLAPWENVDAVAQILAPGGVFIAYVAT
                     TTQLSRLTEDLREHGGFTEPTASETLLRTWHLEGLAVRPDHRMIGHTGFLLATRRMAP
                     GVDAPLRTRRPAKGSYPVDDEWTPDDLGERAVSEKKIRKVRRDVEGRSVEGQGVKGRG
                     VNEIGVDGQSTGGADE"
     gene            702..>1808
                     /gene="arc"
                     /locus_tag="VF362_03620"
     CDS             702..>1808
                     /gene="arc"
                     /locus_tag="VF362_03620"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HEX7590048.1"
                     /translation="MSIPDDVGRLQKQNAELGRLLESARAQLGELRERLGELSAPPQT
                     FGTLLAWAGDHADVMIAGRRMRVAVAPSVARDLPPGQEVLVNALSVVVDVAGPERAGQ
                     AAIVREVIDEERILVVARGEDERVALFLGGNARTRIQEGDTVIIDPRTGFANERVERT
                     GIESLLLEEVPDVDYASIGGLSRQIERIRDAIELPVRHPDLYREHALQPPRGLLLYGP
                     PGCGKTLIAKAVAHSLADAVGTQKSYFLSVKGPELLNKYVGETERYIRTIFERGRAKA
                     ASGAPVVIFFDEMDALFRARGSGVSSDMETTIVPQLLTELDGIESLGNVIVIGASNRE
                     DMIDPAILRPGRLDVKIEISRPDREASVDILGKYL"
BASE COUNT          280 a          595 c          667 g          266 t
ORIGIN      
        1 ggcgtcgggt cgggggcgct cacgatgtcg ctgctgcgcg ccgtcggcga cgggggctcg
       61 ctgctctccg tcgagcgtcg cgaggatttc gccgcgatcg cccgggcaaa cgtcgaggcc
      121 gtcttcgggg gtccgcaccc cgcgtggcaa ctcgaggtcg gggacttcgc ggacgtcgcg
      181 accgcgcggt gcgagccggg ttcggtggac cgcgtcgtgc tggacatgct cgcgccgtgg
      241 gagaacgtcg acgcggttgc ccagatactt gcgcccggcg gcgtgttcat cgcctacgtc
      301 gcgaccacga cccaactgtc ccgcctcacg gaggacctcc gcgagcacgg cggattcacc
      361 gagccgacgg cgtccgagac gctgctgcgc acctggcacc tcgagggcct cgcggttcgc
      421 cccgaccatc ggatgatcgg tcacaccggg ttcctgctcg ccacgcgcag gatggccccc
      481 ggggtcgacg cgccgctgcg cacgcggagg ccggccaagg gctcgtaccc cgtcgatgat
      541 gaatggacgc ccgacgacct cggcgagcgc gcggtgagcg agaagaagat tcgcaaggtg
      601 cggcgggatg ttgaagggcg cagcgttgag gggcaaggcg tgaaagggcg aggtgttaac
      661 gaaatcggcg tcgacgggca atcgacgggg ggagcggacg aatgagcatt ccggatgacg
      721 tggggcgact gcagaagcag aacgccgagc tcgggcggct tctggagtcc gcgcgcgcgc
      781 agctaggcga gctgagggag cgcctgggcg agctctccgc gccgccgcag acgttcggca
      841 ccctcctcgc ctgggcgggg gatcacgccg acgtcatgat cgccgggcgc aggatgcggg
      901 tcgcggtcgc gccctccgtc gcgagggacc tgcctcccgg ccaggaggtg ctcgtcaacg
      961 cgctgagcgt ggtcgtggac gtcgcgggac cggagcgggc gggccaggcg gcgatcgtcc
     1021 gagaggtcat cgatgaggag cgcatcctcg tggtggcgcg cggcgaggac gagcgggtgg
     1081 ccctgttcct gggcgggaac gcgcgcacca ggatccaaga gggcgacacc gtcatcatcg
     1141 atccgcgcac gggcttcgcg aacgagcgcg tcgagcgcac ggggatcgaa tcgctgttgc
     1201 tcgaggaggt tcccgacgtc gactacgcgt cgatcggggg cctctcccgg cagattgagc
     1261 gcatccgcga cgcgatcgaa ctccccgtgc ggcaccccga cctttaccgc gagcacgcgc
     1321 tccagccgcc tcgcggccta cttctctacg gcccgccggg gtgtggcaag accctcatcg
     1381 cgaaggccgt cgcccactcg ctcgccgacg ccgtcgggac ccagaagagt tacttccttt
     1441 cggtcaaggg cccggagctc ctgaacaagt acgtgggcga gacggagcgg tacatccgga
     1501 cgatcttcga gaggggccgc gccaaggcgg cgagcggcgc cccggtggtc atcttcttcg
     1561 acgagatgga cgcgctcttc cgcgcgcggg gatcgggcgt ctcgtccgac atggagacga
     1621 cgatcgtccc ccagttgctg accgagctcg acggaatcga gtcgctcggg aacgtcatcg
     1681 tgatcggcgc gtcgaaccgc gaggacatga tcgaccccgc gatccttagg cctggtcggc
     1741 tcgacgtcaa gatcgagatc tcgcgtccgg atcgcgaggc ctcggtggac atcctcggga
     1801 agtacctg
//
LOCUS       DASVKP010000178         5815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_965523, whole genome shotgun sequence.
ACCESSION   DASVKP010000178 DASVKP010000000
VERSION     DASVKP010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965523"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VF362_03625"
     CDS             complement(<1..550)
                     /locus_tag="VF362_03625"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEX7590049.1"
                     /translation="MKRTTSVVASIATLALTASLAGCKVVPPSFKGTPVTEDRTVTAF
                     DSIELRGVGDITVVAGQELSLSVRADKSAIAGVRTSVEGTTLVINEDDKANSGRVEIV
                     IHVPTLSSVEVLGAGTITVTGLDVERFAIAVAGAGDATVSGRATTVVAVLTGAGSIDA
                     SALKGTDVTARLAGVGSLSLDAS"
     gene            736..1605
                     /locus_tag="VF362_03630"
     CDS             736..1605
                     /locus_tag="VF362_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7590050.1"
                     /translation="MKQRTLGNRTVGAIGLGGMPMSIEGRPDEARSIATIHAALDAGV
                     TLIDTADSYHRDAGEVGHNELLIAKALASYGPGAREVFVATKSGHLRPGDGSWTQNGD
                     PEYVKAAARASAKRLGVESIALHQFHRPDPKVPYADSIGALADLLEEGVIQMAGISNA
                     SIAQIKIAQGILGDALISVQNQFSPGVVWGYPELRYCAEVGLAYLPFSPLGGIGVAGA
                     LGETNAEFGRVAGAHGVSPQQVALAWELALADVVIPIPGASRPESITDSAKAPGLVLT
                     PEEMTALSAVLTR"
     gene            complement(1609..1681)
                     /locus_tag="VF362_03635"
     tRNA            complement(1609..1681)
                     /locus_tag="VF362_03635"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1646..1648),aa:Arg,seq:ccg)
     gene            1788..3461
                     /gene="argS"
                     /locus_tag="VF362_03640"
     CDS             1788..3461
                     /gene="argS"
                     /locus_tag="VF362_03640"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012398030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX7590051.1"
                     /translation="MTPEELSATIRACLDDAVAAGEITLDASALPEVRVERPKDRDHG
                     DWATNVAMQVAKAAGAKPRDVAGHLASRLADRPGIASAEVAGPGFLNIRLAAGAAGEL
                     ARAIVAAGPYYGRGDAMKGLTVNLEFVSANPTGPIHLGGTRWAAVGDSLARLLESQGA
                     KVIREYYFNDHGEQIDHFAESLLAVVLGEDTPEGGYAGQYIQDIADEVMVEAMEAGDK
                     DPIERPRAEALEFFRDRGVKLMFAQIKESLHNFGVNFDVYFHEESLHGSGAVDTCLKE
                     LKASGALYESEGAWWLRSTDQGDDKDRVVIKSDGTAAYIAGDIAYIRDKRKRGADLCV
                     YLLGADHHGYIARLKAAAVALGYPASSVEVIIGQMVNLVKGGEAVRMSKRAGTVVTME
                     DLVEAVGVDAARYALTRSSIDATIDIDLDLLASHTNANPVFYVQYAHARAGAVARNAA
                     EYGISRDQAFAPETLTHETETALLGLLGEFPRVVAQAAQFREQHRVARYLEELAGAFH
                     GWYDRTRVTPQGDEPVTDLHRTRLWLNDATRQVLANGLGLLGVGAPERM"
     gene            3464..4840
                     /gene="lysA"
                     /locus_tag="VF362_03645"
     CDS             3464..4840
                     /gene="lysA"
                     /locus_tag="VF362_03645"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX7590052.1"
                     /translation="MGTGSEHSEFGAAGSAHVWSRTVERGADGVLLVGGCDVTALAAE
                     YGTPLYVVDEEDMRGRAAEFRDEFASAFAGVGAKCSVFYAGKAFLCSQVTRWMRDEGL
                     SMDVASGGELALALRGGMPPARIAMHGNNKSDEEIVAALTVGVGRIVLDSLAEIEVIA
                     AAAESLGVVAPVAVRVTTGVHAGGHEYIATAHEDQKFGLSLATGAALEAMRRCHEAPS
                     IELTGIHTHIGSQILSVEAFQQSAERMLGLRAQFARETGIEVPEVGLGGGYAIAHAQG
                     DEELGAAEAARGIAAAVAGAMAAVGGTWPSFTIEPGRAIAGPAGMTLYTVGVTKVVRL
                     EDGGERRYVAIDGGMSDNMRPITYGAEYTATLASRVSAAAPVLSRVVGKHCESGDIIV
                     QNIDLAGDVDRGDILAVPATGAYGRVMSNNYNALLRPGVVAVRGGAAHELVRRDTIED
                     LLAWDVAP"
     gene            complement(4846..5430)
                     /locus_tag="VF362_03650"
     CDS             complement(4846..5430)
                     /locus_tag="VF362_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590053.1"
                     /translation="MTPDAHHSGEAVTTPSEPAKVLAVCTGNICRSPAMEFYLKRAWG
                     ADAVVTSAGTIAATGWHIPAPMRAVEEAAGLVVPDHDPRQLDVAIIEAQDLVLVTTHS
                     HKEWIERHVGAVPPNTFLLTEAAALTTVARRPVGDSLADRIRTAASLLSAARSLDDEV
                     HPDVDDPYLHGDEAYGTAMKEIAENLDVLIAWVG"
     gene            5486..>5815
                     /locus_tag="VF362_03655"
     CDS             5486..>5815
                     /locus_tag="VF362_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX7590054.1"
                     /translation="MADDERRVRIAILGAGTVGSQVARVLIEQKDDLAQRIGAIPEII
                     GIAVLDPDEVRTDDLPRSLLTSDIGALVAEADIIVELMGGIEPARTVLLRALNGGKSV
                     VTANKALL"
BASE COUNT          895 a         1977 c         2099 g          844 t
ORIGIN      
        1 gggaggcgtc gagcgaaagc gacccgaccc ccgccagccg ggcggtcacg tcggtcccct
       61 tgagggcgct cgcgtcgatc gagccggcgc cggtcaggac cgcgacgacc gtcgtggccc
      121 ggcccgagac cgtcgcgtct ccggcccccg cgacggcgat ggcgaagcgc tcgacgtcga
      181 ggcccgttac cgtaatggtg cccgccccga gaacctccac ggacgagagc gtcggcacat
      241 ggatgacgat ctccacgcgt ccgctgttgg ccttgtcgtc ctcgttgatg acgagggtcg
      301 tgccctccac gctcgtccgg acgccagcga tcgcggactt gtcggcgcgg acgctgagcg
      361 acaactcctg tcctgcaacg acggtgatgt cgccgacgcc gcgcagttcg atcgagtcga
      421 acgcggtgac ggtgcggtcc tccgtgacgg gggtcccctt gaaggacggc ggaacgacct
      481 tgcatcccgc gagggaggcg gtgagggcga gggtcgcgat ggacgcgacg acggaggtgg
      541 tgcgcttcat ggctgctcct tgagtggcta gtttgacgct atcgccagcg ccgccggttg
      601 cgcatcacgc gcatggatga atggcggagg gcgcgtctcg ggggaatccc tgggggtgat
      661 ctcaggggga gtaggctcta tcgaaacgtt acgaccacgg cacaggattc aatcacggag
      721 caggaggtga gcccgatgaa gcagcgaacc ctaggaaacc gaaccgtcgg cgcgatcggg
      781 ctcggcggca tgccgatgtc catcgagggc cggcccgacg aggcgcgttc catcgcgacc
      841 atccacgccg cgctcgacgc gggggtgacg ctgatcgata cggcggactc ctaccaccgg
      901 gatgcgggcg aggtcgggca caacgaactc ctcatcgcca aggccctcgc gtcctacggc
      961 cccggcgcgc gcgaggtctt cgtcgcgacc aagagcggcc acctgcgccc cggtgacggg
     1021 agctggacgc agaacggcga ccccgagtat gtgaaggcgg cggccagggc ctcggccaag
     1081 cgcctcggcg tcgaatccat cgcgctccac cagttccacc gccccgaccc caaggtgccg
     1141 tacgcggact caatcggcgc gctcgcggac ctgctcgagg agggcgtcat ccagatggct
     1201 ggcatctcca acgcgagcat cgcgcagatc aagatcgccc agggaatcct cggagacgcg
     1261 ctgatctccg tccagaacca gttctcgccc ggcgtcgtgt gggggtaccc cgagttgcgg
     1321 tactgcgccg aggttggtct cgcgtacctt ccgttcagcc cgctcggcgg gatcggcgta
     1381 gcaggggcgc tcggagagac caacgcggag ttcggccggg tcgcgggggc ccacggcgtg
     1441 agcccgcagc aggtcgcgct cgcgtgggag ctggcgctcg ccgacgtcgt cattccgatt
     1501 cccggagcct cgcgccccga gtcgatcacc gactcggcca aggccccggg cttggtcctg
     1561 acgcccgagg agatgacggc gctgagcgcg gtgctgaccc gctaggggtg ccctcgacag
     1621 gagtcgaacc tgcgaccttc ggtaccggaa accggcgctc tatccgctga gctacgaggg
     1681 cggtagcggg cgatgcccgc agcagttgag actaccagcc ccgcgctttc tccttcgcgg
     1741 ttggaaacga cgaaccgtcc gcccgcgcgc gtggatagac tcggcgggtg acccccgaag
     1801 aactgtccgc gaccattcgc gcgtgcctgg acgacgccgt cgcggctggc gagatcaccc
     1861 tggacgcctc cgcccttccc gaggtgaggg tcgagcggcc gaaggatcgc gaccacggcg
     1921 actgggcgac gaacgtcgcg atgcaggtgg cgaaggccgc gggtgcgaag ccgcgcgacg
     1981 ttgccggcca cctcgcctcg cgcctcgcgg accgtcccgg tatcgcgtcg gccgaggtcg
     2041 cggggccggg gttcctcaac atccgcctcg cggcgggagc ggcgggtgag ctcgcgcgtg
     2101 ccatcgtcgc ggcggggccg tactacgggc gcggcgatgc gatgaagggc ctcacggtca
     2161 accttgagtt cgtttccgcc aatccgacgg ggccgatcca ccttggcggc acgcgctggg
     2221 ccgcggtcgg ggatagcctg gcgcgcctgc ttgaatccca gggcgcgaag gtgatccgcg
     2281 agtactactt caacgaccac ggcgagcaga tcgaccactt cgccgagtcg ctcctcgccg
     2341 tggtcctggg cgaggatacg ccggagggcg gctacgcggg ccagtacatc caggacatcg
     2401 ccgacgaggt gatggtcgag gccatggagg cgggtgacaa ggacccgatc gagcgcccgc
     2461 gcgcagaggc gctcgagttc ttccgcgacc gcggcgtcaa gttgatgttc gcccaaatca
     2521 aggagtcgct ccacaacttc ggcgtcaact tcgacgtcta cttccacgag gaatccctcc
     2581 acggctccgg ggccgtggac acgtgcctca aggagttgaa ggcctccggc gcgctctacg
     2641 agagcgaagg agcgtggtgg ctccgttcca ccgaccaggg cgacgacaag gaccgcgtcg
     2701 tcatcaagtc ggacggcacg gccgcgtaca tcgcggggga catcgcctac atccgcgaca
     2761 agcgcaagcg cggcgccgac ctctgcgtct acctgctcgg cgcggaccac cacggctaca
     2821 tcgcgcggct caaggccgcg gccgtcgccc tcggctaccc cgcgtcgtcc gtcgaggtga
     2881 tcatcgggca gatggtcaac ctcgtgaagg gcggcgaggc tgttcggatg tccaagcgcg
     2941 ccggaacggt cgtcacgatg gaggacctgg tcgaggcggt cggcgtcgac gcggcgcggt
     3001 acgcgctcac gcgatcgagc atcgacgcga ccatcgacat cgacctggac ctgctcgcgt
     3061 cccacacgaa cgcgaacccc gtcttctacg tccagtacgc gcacgcccgc gcgggggcgg
     3121 tggcccgcaa cgccgccgag tacgggatct cccgcgatca ggccttcgcg cccgagaccc
     3181 tcacgcacga gaccgagacg gcgctactcg gcctcctggg cgagttcccg cgcgtcgtcg
     3241 cgcaggccgc gcagttccgc gaacagcacc gtgtggcgcg ctaccttgag gaactcgccg
     3301 gcgccttcca cggctggtac gaccgcacgc gcgtcacgcc tcagggggac gagccggtca
     3361 ccgaccttca ccgcacccgc ctttggctca acgacgcgac gcgccaggtc ctcgccaacg
     3421 gccttggcct gctgggcgtc ggcgcccccg aaaggatgta gggatgggca cggggtccga
     3481 gcacagcgag ttcggtgccg caggctccgc gcacgtatgg tcgcgcacgg tcgagcgcgg
     3541 ggcggacggc gtcctcttgg tggggggctg cgacgtgacc gcgctcgccg ccgaatacgg
     3601 cacgcccctg tacgtggtcg acgaggaaga catgcggggc cgcgcggccg agttccgcga
     3661 tgagttcgcc tccgccttcg ccggggtcgg cgcgaagtgc tccgtcttct acgcgggcaa
     3721 ggcgttcctg tgctcgcagg tgacgcgatg gatgcgggac gagggcctgt cgatggacgt
     3781 cgcttcgggg ggcgaacttg cgctcgcgct gcgcggcggc atgcccccgg cccggatcgc
     3841 gatgcacggc aacaacaagt ccgacgagga gatcgtcgcc gcgctcacgg tcggggtagg
     3901 gcggatcgtc ctcgactcgc tcgcggagat cgaggtgatc gcggccgccg cggagagcct
     3961 cggcgtcgtc gcccccgtcg cggtgcgcgt gacgacgggc gtccacgcgg ggggacacga
     4021 gtacatcgcc accgcccacg aggaccagaa gttcggcctc tccctcgcca cgggcgccgc
     4081 gctcgaggcg atgcgccgtt gccacgaggc gccctcgatc gagctgaccg ggatccacac
     4141 ccacatcggg agccagatcc tctccgtcga ggcattccag cagagcgccg aacggatgct
     4201 cggcctccgg gcacagttcg cgcgcgagac ggggatcgag gttccggagg tggggctcgg
     4261 gggcgggtac gcgatcgcgc acgcgcaggg ggacgaggaa ctcggcgcgg cggaggccgc
     4321 gaggggcatc gccgccgccg tcgccggagc catggccgcc gtcggcggga cgtggccgtc
     4381 gttcaccatt gagccggggc gcgcgatcgc cggccccgcg gggatgacgc tctacaccgt
     4441 cggcgtgacc aaggtcgttc gcctggaaga cgggggagag cgtcggtacg tcgcgatcga
     4501 cgggggcatg agcgacaaca tgcggcccat cacgtatggc gccgagtaca cggccaccct
     4561 tgcgtcccgc gtgagcgccg ccgcgccggt cctctcgcgg gtggtgggca agcactgcga
     4621 gagcggcgac atcatcgtgc agaatatcga cctggccggg gacgtggatc ggggcgacat
     4681 cttggccgtc cccgcgacgg gcgcgtacgg ccgggtgatg tccaacaact acaacgcgct
     4741 cctgcggccc ggggtcgtcg cggtgagggg cggcgccgcc cacgagttgg tgcgccgcga
     4801 cacgatcgag gacctgcttg cctgggacgt cgcgccgtag gcgctctaac ccacccacgc
     4861 gatcagcacg tcgaggttct ccgcgatctc cttcatggcc gtgccgtagg cctcgtcgcc
     4921 gtggaggtag gggtcgtcga cgtcggggtg cacctcgtcg tccagcgacc gcgccgcact
     4981 caggagcgag gcggcggtgc ggatccggtc cgcgagggag tcgcccacgg gcctgcgcgc
     5041 gacggtcgtg agggcggccg cctccgtcag gaggaaggta ttggggggca cggcgccaac
     5101 gtggcgctcg atccactcct tgtgggagtg ggtggtcaca aggacgagat cttgcgcctc
     5161 gatgatggcg acgtcgagct ggcggggatc gtggtcggga acgaccagtc ctgcggcctc
     5221 ctcgaccgcg cgcatcggag cggggatgtg ccagccggtc gcggcgatgg tgcccgcgga
     5281 ggtgacgacc gcgtcggcgc cccacgcgcg cttgaggtag aactccatgg cgggagagcg
     5341 gcagatgttc cccgtgcata cggcgaggac cttcgccggc tccgacggag ttgtcacggc
     5401 ttctcccgag tggtgggcat cgggcgtcat ggtgaagagc ctactggggc gtcgcggctc
     5461 gcggcgctac agatagagtt ggggcgtggc agatgacgag cgccgagtgc gcatcgcgat
     5521 cctcggggcc ggcaccgtcg gctcccaggt ggcgcgagtc ctgatcgagc agaaggacga
     5581 cctcgcgcaa cgcatcggcg cgattcccga gatcatcggc atcgccgtcc tcgatcccga
     5641 cgaggtgcgc actgacgacc ttccccgctc cctcctcacg tcggacattg gcgcgctcgt
     5701 cgccgaggcg gacatcatcg tcgagttgat gggcgggatc gagcccgccc gcaccgtcct
     5761 gctgcgcgcg ctcaacggcg gcaagtccgt ggtgacggcg aacaaggccc tcctt
//
LOCUS       DASVKP010000179         2959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1212616, whole genome shotgun sequence.
ACCESSION   DASVKP010000179 DASVKP010000000
VERSION     DASVKP010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212616"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..360)
                     /locus_tag="VF362_03660"
     CDS             complement(28..360)
                     /locus_tag="VF362_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590055.1"
                     /translation="MVFMKAPSRNYSQDVARALDPSVRGSELVVLAAHRDPGVRAAVA
                     SRPDAPMASLISLIHERDLRILSALVDNPASPPTVIRALTAERRSDIGERASARVRAA
                     GPHGEVAF"
     gene            complement(502..1314)
                     /gene="map"
                     /locus_tag="VF362_03665"
     CDS             complement(502..1314)
                     /gene="map"
                     /locus_tag="VF362_03665"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX7590056.1"
                     /translation="MVRDRIQYKTRDQMRLMALAGVVVEQALAAAASRAVAGATTRDL
                     DRAAAAVIGERGASPSFLGYHGYPATVCVSVNDEVVHGIPGDRILAEGDVVSIDCGAI
                     VEGWHGDSAVSLVVGRARNPGDEELVAATREALWDGIAALASARTIADVAGAIEDAAT
                     HAGLVPVEGYVGHGIGTEMHQGPEVPNERLRGREPRVRPGLCVAIEPMLTPGGRDTVV
                     LEDGWTVTTADGSQAAHWEHSVALHDGGIWVLTASDGGAGELARRGIVPVAP"
     gene            complement(1320..1889)
                     /locus_tag="VF362_03670"
     CDS             complement(1320..1889)
                     /locus_tag="VF362_03670"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX7590057.1"
                     /translation="MMRIVLLGPPGAGKGTQAVRLAERWGIPAISTGDIFRANVSGGT
                     PLGEQAKRYMDAGELVPDEVTNAMVRNRLEDADTAAGFLLDGFPRNPEQAVELDAMLA
                     ELAVSLDAAIELTADADAVTERILKRAEIEGRADDREDVIRERLRVYAEKTAPVTAFY
                     EGKGLLVRADGMGAIDDVTERIIAAVEGR"
     gene            complement(1886..>2959)
                     /gene="secY"
                     /locus_tag="VF362_03675"
     CDS             complement(1886..>2959)
                     /gene="secY"
                     /locus_tag="VF362_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009884968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX7590058.1"
                     /translation="FALGIMPYITASIMVQLLRVLIPRFEALQKEGADGQAKLTQYTR
                     YITIGFAAMQSASYITLARKGLLLQNCTDPLLTSETWYAISIMILTMTAGTILIMWLG
                     ERITERGIGNGMSLLIFTSIASGFASAGVSLRESSGQFNGLWVFLTVLVVVVVIVFIE
                     QSQRRIPVQYAKRMVGRKMLGGTSTYIPIKINMAGVIPVIFASSLLTLPQLVTQFSHS
                     NAGWAVWIQRYVTPTSAPLNMLMYSLFIIFFAYFYTAITFNPDEVADNMKKYGGFIPG
                     IRPGKPTAEYLDFVLSRITAAGALYLAIVALVPTILLVALNLNQRLPFGGSSLIIVVG
                     VGLDTVKRIQSQLEQRHYEGFLK"
BASE COUNT          512 a          968 c         1009 g          470 t
ORIGIN      
        1 cgcgcctcgg cgcgaccgag agtccgccta gaaggcgacc tcgccgtggg ggcccgcggc
       61 gcgcacgcgc gcagacgcgc gctccccgat atcggagcga cgctcggccg tcagggcgcg
      121 aatcaccgtt ggcggactcg ccgggttgtc gaccaatgcg gagagaatcc gcagatctct
      181 ctcgtgaatg agagagatca gcgacgccat cggcgcgtct gggcgcgagg cgacggcggc
      241 acggacgccc ggatcgcggt gcgccgcgag cacgacgagt tccgaaccgc gaaccgatgg
      301 atcgagcgcg cgagcgacat cctgcgagta gttgcgagat ggggccttca tgaacaccat
      361 ggcaacggtc cttccgcgga ggagatcggg gttcgatgcc gacgaggatg ccggcctcgt
      421 tgtcttggct tcagcgtaag cgaccaacgt tgccgcgccg tggccgccag atgaacagtg
      481 gatgaagggg gcccaccccg gctagggggc gacgggaacg attccgcgcc gagcgagttc
      541 gccggccccg ccgtcggatg ccgtgagcac ccagattccg ccgtcgtgaa gcgcaaccga
      601 gtgctcccag tgggccgcct gcgagccgtc cgccgtcgtc accgtccagc cgtcctcgag
      661 cacgaccgtg tctcgtccgc cgggcgtcag catcggctcg atcgcgacgc acagacctgg
      721 gcgcacgcgt ggctctcgcc cccgcaggcg ctcgttgggc acctcggggc cctgatgcat
      781 ctccgtgcca atcccgtggc ccacgtagcc ttctacgggc acgagaccgg catgcgtggc
      841 cgcgtcctca atcgcccccg cgacgtcggc gatggtccgg gcgctcgcga gcgcggcgat
      901 gccgtcccag agcgcctcgc gcgtggcggc gaccagttcc tcgtcgccgg ggttccgggc
      961 ccgcccgacg acaagactca cggcggagtc gccgtgccag ccctcgacga tggcgccgca
     1021 gtcaatggag acgacgtcgc cctcggcgag gattcgatct ccggggatcc cgtggacgac
     1081 ctcgtcattg acggacacgc agaccgtcgc ggggtacccg tggtagccga ggaagcttgg
     1141 ggaggccccg cgctcgccga tgacggccgc ggccgcgcga tcgaggtccc gcgtcgtggc
     1201 accggccacg gcgcgggagg cagccgcggc aagtgcctgt tcgacgacga cgccagccag
     1261 cgccatgagg cgcatctggt cgcgcgtctt gtactgaatg cggtcgcgga ctatgacggc
     1321 tagcgccctt cgaccgccgc gatgatccgc tcggtcacgt cgtcgatcgc tcccatgcca
     1381 tccgcccgca ccaggaggcc cttgccctcg tagaacgcgg tcaccggggc ggtcttctcc
     1441 gcgtacaccc gcagtcgctc gcggatgaca tcctctcggt cgtcggcgcg cccctcgatc
     1501 tcggcccgct tcagaatgcg ttccgtgacc gcgtcagcat cggcggtaag ttcgatcgcg
     1561 gcatcgaggc tgaccgcaag ttccgcgagc atcgcatcga gctcgacggc ctgttcgggg
     1621 tttcggggaa agccatcgag aaggaagccc gcggccgtgt cggcgtcctc aaggcggttg
     1681 cgaaccatcg cgttggtcac ctcgtccgga accaactcgc ccgcgtccat gtagcgcttc
     1741 gcctgctcgc ccagcggggt gccgcccgag acgttggcgc ggaagatgtc tcccgtggag
     1801 atggcgggga tcccccaccg ctcggcaagg cggacggcct gggtcccctt gcctgcgccg
     1861 ggggggccga ggaggacgat acgcatcatt tgaggaaccc ttcgtagtga cgttgctcca
     1921 gttgagactg gatgcgcttc acggtgtcga gacccacgcc gacgacgatg atcagcgacg
     1981 agcccccgaa cggtagtcgc tggttgaggt tgagcgccac caagagaatg gtcgggacga
     2041 gcgcgacgat cgcaagatag agggcacccg cggccgtgat acgtgacagg acgaagtcaa
     2101 gatactcggc ggtcggcttg cccgggcgaa tcccggggat gaagcccccg tacttcttca
     2161 tgttgtcggc aacctcgtcc ggattgaacg tgatcgccgt gtagaagtac gcgaaaaaga
     2221 tgatgaagag cgagtacatg agcatgttca ggggggcgct cgtcggcgtc acgtaccgct
     2281 ggatccacac cgcccacccc gcattgctgt gggagaactg agtcacaagt tggggcagcg
     2341 tcaagaggga cgaagcgaag atgacgggga tcacgccggc catgttgatc ttgatgggga
     2401 tatacgtcga cgtgccgccc agcatcttgc gacccaccat ccgtttcgcg tattggacgg
     2461 ggatgcggcg ctgggactgc tcgatgaaga cgatgacgac cacgaccacg agcacggtga
     2521 ggaataccca caggccgttg aactgcccgc tcgactcgcg aagcgaaaca ccggctgagg
     2581 cgaagcccga cgcgatcgag gtgaagatca gcagggacat tccgttgccg atgccccgtt
     2641 cggtgatgcg ctcgccgagc cacatgatga ggatggtccc ggccgtcatc gtgaggatca
     2701 tgatcgagat ggcgtaccag gtctcgctcg tcaggagcgg gtccgtgcag ttctgcagca
     2761 acaggccctt gcgcgcgagc gtgatgtacg acgccgactg catcgcggcg aacccgatcg
     2821 tgatgtaccg cgtgtattgc gtgagcttcg cctggccgtc cgcgccttcc ttctggaggg
     2881 cctcgaaacg agggatgagc acgcgcaaca actgcaccat gatcgatgcg gtgatgtacg
     2941 gcatgatgcc cagcgcgaa
//
LOCUS       DASVKP010000180         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_387463, whole genome shotgun sequence.
ACCESSION   DASVKP010000180 DASVKP010000000
VERSION     DASVKP010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387463"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..972)
                     /locus_tag="VF362_03680"
     CDS             complement(94..972)
                     /locus_tag="VF362_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEX7590059.1"
                     /translation="MENIDDPWASLAVVTGLEALTLRKLGPTTFEGDSLPVPGGRVFG
                     GQVLGQGVVAAGHTVDEDRHIHSLHGYFLRAGDATKPIRFEVELLRDGGSFSARRVHA
                     LQDGRPLLSMITSFQLQQDGPEHSVTMPEVPGPETLPSGIDVLRPFEGHPMADFWLKE
                     APFDVRQVEGSIFYQADPRPKPYQTAWMRVREPLDVPELTHRALIAFGCDQVILEPLL
                     RRHGLPWTTKNLAFASLDHAMWWHRPARADEWLLFVQDAPTAQGGRGLAGAWVFSEDG
                     RLVATIAQEGMLRLGE"
     gene            1119..1802
                     /locus_tag="VF362_03685"
     CDS             1119..1802
                     /locus_tag="VF362_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590060.1"
                     /translation="MSAITDARSAVTRSAGSFARIERPRPAKVARALLWLAIDRAADT
                     RSVSGAARQWRRSVSVTICRASLALLVVWVFVVVWPGAPHMAGEADPRPLWAAAPVIA
                     YLLSGGLVIDQFGARYGAAALFGLHLGGVVGFLPVMWAIALANPSLDATRFGQSTMAL
                     AAAWPVEFIGLVLLSYALAWLVPGSLPRQFRFTREAWGIVRDVDSARPHRPLPLTEAR
                     SEASVGAWE"
     gene            1793..2311
                     /locus_tag="VF362_03690"
     CDS             1793..2311
                     /locus_tag="VF362_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590061.1"
                     /translation="MGVTKPQVRAILWLLAGGVGHEVIESVLSPRLDAALPPGGWHQL
                     GVPPAMFIPIEIAGLLVACAALAGLAAVISAGMLWRHFDLAADLPVRFAYLGAWLFLA
                     SWPLETVFLFIRGNHVPGSPSGAMIALASAGLLACQAVALWQVWRARRDFRAASRDDA
                     GDDVVDDVVETV"
     gene            complement(2541..>3081)
                     /locus_tag="VF362_03695"
     CDS             complement(2541..>3081)
                     /locus_tag="VF362_03695"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX7590062.1"
                     /translation="LTFLTVLREYGVDITSLLAGGAIVGVALGFGAQSLVKDLLSGMF
                     MLLEDQYGVGDIVDVGEASGVVEAFGLRATRLRSLDGTVWFIPNGEIRRVGNMSRLWS
                     RAMIEIRVDFDEDVDAAREAILDAAREAMARPDIAPHVLGEATVPGIEGLESDAVVLR
                     LMVQVQPAKQWDVQRAIRA"
BASE COUNT          406 a         1119 c         1078 g          478 t
ORIGIN      
        1 cgaggagaac aagattgagc gccttggccc ggaggccgcg cgcccgcacc gcgtcacgta
       61 ccgcaagctc acgcggggat agcgagcgcg gcgctactcg ccaagccgca gcatcccctc
      121 ctgggcgatc gtcgcgacga ggcgcccatc ctcggagaac acccacgcgc ccgcgaggcc
      181 gcggccgccc tgtgcggtcg gggcgtcctg gacgaagagc agccactcgt ctgcgcgcgc
      241 cgggcggtgc caccacatcg cgtgatcaag gctcgcgaac gcaaggttct tggtcgtcca
      301 cgggagcccg tgcctccgca gtagcggctc gaggatcacc tggtcgcatc cgaaggcgat
      361 gagcgcgcgg tgggtgagtt cgggcacgtc gagcggctcg cgcacgcgca tccacgccgt
      421 ctgatagggc ttgggacgcg ggtccgcctg gtagaagatc gagccctcga cctggcgcac
      481 gtcgaacggc gcctccttga gccagaagtc cgccatcggg tgcccctcga agggccgcag
      541 gacgtcgatg ccggagggga gggtctcggg tccgggcacc tctggcatgg tgacggaatg
      601 ctcggggccg tcctgctgga gctggaacga cgtgatcatg ctcagcagcg ggcggccgtc
      661 ctggagggca tgcaccctgc gggcggagaa cgagccgccg tcgcggagta gctcgacctc
      721 gaaacgaatg ggcttggtgg cgtcgcccgc gcggaggaag tagccgtgca gcgagtggat
      781 gtgacggtcc tcgtccaccg tgtgacccgc cgcgacgacg ccctgaccca gcacctggcc
      841 gccgaagacg cgcccgcccg gcacgggcag ggagtcgccc tcgaacgtcg tcgggccgag
      901 tttcctgagg gtcagggcct cgagcccggt cacgacggct agcgaggccc acgggtcgtc
      961 gatgttctcc acgcgcgcca cccttctccc ggtgcatcga ggctaccgcg cgtgcgccca
     1021 ggggcgcttc gcaccgcgtc gcgcccgccc ggaacgtcct tgccgccgcg agcgttcccc
     1081 ccatgacacc ccacctcgta gcgaaagggg gtcgcgccgt gtccgcaatc accgatgcgc
     1141 gttcggccgt gacccgaagc gccggaagtt tcgcccggat cgagcgcccc cgacccgcca
     1201 aggtggcgcg cgccctgctc tggctcgcga ttgatcgcgc ggccgacacc cgctccgtct
     1261 cgggcgcggc gcgacagtgg aggcgcagcg tatcggtcac aatctgccgc gcgtcgctcg
     1321 cccttctcgt ggtctgggtg ttcgtcgtgg tctggcccgg cgccccgcac atggcaggcg
     1381 aggctgaccc tcgtccgctc tgggccgccg cgcccgtcat cgcctacctg ctctcgggcg
     1441 ggctggtcat cgaccagttc ggtgcgcgct acggcgcggc cgcgctcttc gggcttcacc
     1501 tcggcggcgt cgtggggttc ctgcccgtca tgtgggcgat cgcgctcgcg aacccgagcc
     1561 tcgatgcgac gcggttcggc cagtcgacca tggctctcgc cgcagcatgg cccgtcgaat
     1621 tcatcggcct cgttctgctg agttacgcgc tggcatggct cgtccctggc tccctcccgc
     1681 gccagttccg gttcacccgc gaggcgtggg gaatcgtccg cgacgtcgac tcggcgcgcc
     1741 cgcaccggcc gctgccgttg acagaagccc ggagcgaggc cagcgtcggg gcatgggagt
     1801 gacgaaaccg caggtgcgtg cgatcctgtg gctcctcgcg gggggcgtcg gacacgaggt
     1861 catcgagagc gtcctgtcgc cgaggctcga cgcggcgctc cccccgggcg ggtggcatca
     1921 actcggcgta cctcccgcga tgttcattcc gatcgagatc gcggggctgt tggtcgcgtg
     1981 cgccgcgctc gctgggctcg ccgccgtcat tagcgccggg atgctctggc ggcacttcga
     2041 ccttgccgca gacctacccg tgaggttcgc ctatctcggc gcgtggctct ttctcgccag
     2101 ttggccgctc gagaccgtct tcctcttcat ccgcggcaac cacgtccccg gatcgccgtc
     2161 gggggccatg atcgcgctcg cgagcgcggg cctgcttgcc tgccaggccg tcgcgctgtg
     2221 gcaggtgtgg cgcgcccggc gcgatttccg cgcggccagc cgggacgacg caggggacga
     2281 cgtcgtggac gacgtcgtgg agacggtcta ggcgtcgggc gcctcgcccg gctcggttgc
     2341 ggtcacgcgg ttggctcgcc cttgggcgcg cgcgggggcg ccttcttcgc ctgcttggct
     2401 cccgtcggcg tcgccttgcc acctgctggc gccgctttgc cgcccgccgg cgccgcgtcg
     2461 gccgcgggac gcgggcgcgt gtagacccgg ttgaccggca ccgcgagcgt caagccgcgg
     2521 tggcggaact tcttctggat ctaggcccgg atcgcgcgtt ggacgtccca ttgcttggca
     2581 ggctggacct gcaccatgag ccgcaggacc accgcgtcgg actccagccc ctcgatcccc
     2641 gggacggtcg cctcgcccag cacgtgcggg gcaatgtcgg gccgcgccat ggcctcgcgc
     2701 gcggcatcga ggatcgcctc gcgcgcggcg tcgacgtcct cgtcgaagtc cacgcgaatc
     2761 tcgatcatcg cgcgcgacca caggcgcgac atgttgccca cgcggcgaat ctcgccgtta
     2821 gggatgaacc acaccgtccc gtcgaggctc ctcaggcgcg tggcgcgcag gccgaacgcc
     2881 tcgaccacac cgctcgcctc gccgacgtcg acgatgtcgc cgacgccgta ctggtcctcg
     2941 agcaacatga acatcccgct caacaggtcc ttgacgagcg actgggcgcc gaagccgagg
     3001 gcgacgccca cgatcgcccc gcccgcgagc agggacgtga tgtcgacgcc atactcgcgg
     3061 agcaccgtca ggaaggtaag g
//
LOCUS       DASVKP010000181         4401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_719750, whole genome shotgun sequence.
ACCESSION   DASVKP010000181 DASVKP010000000
VERSION     DASVKP010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719750"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VF362_03700"
     CDS             complement(<1..186)
                     /locus_tag="VF362_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590063.1"
                     /translation="MPNDDLRELLIQGYDEHASSMRSGAGFASQYFDGVSRRVTRRRA
                     ARVVAIASGTVASVTVLA"
     gene            complement(196..738)
                     /locus_tag="VF362_03705"
     CDS             complement(196..738)
                     /locus_tag="VF362_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7590064.1"
                     /translation="MAAWQPLLAQLMRERSHLLVAYAGLYTGNVADAEDVVQEALIRS
                     FSRGRSFENVAAADSYVRKAIPCVFIDRARASSSSRRAVRRSVDHAPHTVDIDAQVDV
                     RRALHRLSPRERTCIVLRFFDDMTVPQIATRLGISDGAVKRYLSDASSRLSEHLSIDA
                     DWRSAPESADVVAPTSRITF"
     gene            complement(814..1380)
                     /locus_tag="VF362_03710"
     CDS             complement(814..1380)
                     /locus_tag="VF362_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX7590065.1"
                     /translation="MKPLPRLALLVTYAILFLAAAGRSSVQLTTKASEAPLPYWLSAV
                     AALIYGVIAFALWRATARWRTVALVGTAVELVGVLAVGTWGIVEPSIWPDETVWSGYG
                     SGYGWIPLILPPIALWTLLKARRATGAGAVSVVDTMKAVGEEVAGLTREVVGNSTRDE
                     FMAAEGEAEVIDAQVKLAGDHKKDAPQG"
     gene            complement(1390..1857)
                     /locus_tag="VF362_03715"
     CDS             complement(1390..1857)
                     /locus_tag="VF362_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590066.1"
                     /translation="MRAFSDAVYPAVLLAMVAVLVAGAVAYVAATRIGPTAGAAAQNT
                     ARFARLLIVATWVARGATGIEIVAIGALLGAGVTHPPMIMLVGYLVAAVAVLGLLGIS
                     RLGAPPAPGEAHDPDRPALSPRQMVQADGAAAMIVGAALAVVAWRLNTILLAG"
     gene            complement(1905..2354)
                     /locus_tag="VF362_03720"
     CDS             complement(1905..2354)
                     /locus_tag="VF362_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590067.1"
                     /translation="MRVDAFLADSADVINNKIYALGGGWNTIFARAFPVIHRRLTVAA
                     TVHVPFTDTNTAHKFEIRLVTEDGVDHPIGMRADAEGRPVPVTAMGGEFTLGRPPQLV
                     DGDEQIACFAFTIDGMRFEGPGMFSWVITIDGEEATRLPMRVQEQRG"
     gene            2406..4286
                     /gene="dnaG"
                     /locus_tag="VF362_03725"
     CDS             2406..4286
                     /gene="dnaG"
                     /locus_tag="VF362_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012246275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX7590068.1"
                     /translation="MAGTINRDDIAAVREKAPLEDIVRERVALKQAGVGSLKGLCPFH
                     DEKSPSFHVRPMQGHWHCFGCGEGGDVIAFIMKADGLPFADAVEYLAGRAGVTLRYES
                     GGARGASDGGGGRKRMLEAHRVAAEFFVGQLVTPEAKTGRDFLTGRGFGKEAAAQFGV
                     GYAPKGWSALLDHLRGKGFTEPEIEASGLVSRGQSASGQSARLYDRFRGRLVWPIRAV
                     TGEVIGFGARKLYEDDEGPKYLNTPETALYKKSQVLYGLDLAKKDIASTHRVVVVEGY
                     TDVMACHLSGVTTAVATCGTAFGEEHAKVLRRLLGGDAGAPTKVGGSRGEVIFTFDAD
                     DAGQNAALKAFQLDATFLSQTFVAVGPDGLDPCDLRLRDGDVAVAALVEARVPLYEFV
                     IKSALAGNDLETPEGRVTALRAAAPVVAGIRDAAIGPEYARRLSGWLGMEPAEVMRAV
                     TTAAKAARGAAQGRVDVTAAVAVGPDPKDPVVRAERLALEVLLQMPEKVEPAWAAGIG
                     PETFTVPQYRAVLDGIAAAGGIATAGKDWANRVADEVGEVLAPLVSELIVSPVPQDRP
                     SEMARYVKSVLSRVQDLSLTRRIGEVRGRMQRIGEGNPGYQEAFAELVALEGERRGHR
                     EE"
BASE COUNT          670 a         1526 c         1587 g          618 t
ORIGIN      
        1 agccagcacc gtcacggacg cgaccgtccc gctggcgatc gcgaccaccc ttgccgcccg
       61 gcgccgtgtc acccgtcgag acaccccgtc gaagtactgc gacgcgaaac ccgcgcccga
      121 cctcatggat gaggcatgct cgtcgtagcc ctggatcagc aactccctca gatcgtcgtt
      181 cggcatgatc gctccctaga aggtgatacg gctcgtgggc gccactacgt cggcgctctc
      241 gggggcgctc cgccagtcgg cgtcaatgga caggtgctcg gacaagcgcg aggaggcgtc
      301 cgaaaggtag cgcttgacgg cgccgtcact gatccccagc cgggtggcga tctggggcac
      361 cgtcatgtca tcgaagaacc gcagcacgat gcacgtgcgc tctcgggggg agagccggtg
      421 cagggcgcgg cggacgtcga cctgggcgtc gatgtcgacc gtgtggggcg cgtggtcgac
      481 ggaccggcga accgcacgac ggctcgacga cgacgccctc gcccggtcga tgaagacgca
      541 ggggatcgcc ttgcggacat aggagtcggc cgcggcgacg ttttcaaacg accgaccgcg
      601 actgaacgac cggatcaagg cctcctggac cacgtcctcg gcgtcggcga cattcccggt
      661 gtagaggccc gcgtacgcga caagcaggtg ggagcgctcg cgcatcaact gcgccagcaa
      721 cggctgccac gcggccattc cacctccaac gcctggtcgt gcccgtcaaa cggagaccgc
      781 ggcacaatcg ttggggtctc gcacccccgc gcgtcagccc tggggggcgt ccttcttgtg
      841 atcccccgcc aacttgacct gagcgtcgat gacctcggcc tcgccctcgg ccgccatgaa
      901 ctcgtcgcgg gtcgagttgc cgacgacctc ccgcgtcaag cccgcaacct cttccccaac
      961 ggccttcatc gtgtccacca cgctcacggc gcccgcgccc gtcgcgcgcc gcgccttcag
     1021 aagcgtccac agtgcgatcg gcggcaggat cagcgggatc cagccgtacc cggagccgta
     1081 gcccgaccac acggtctcgt cgggccagat gctcggctcg acgatgcccc aggtcccgac
     1141 ggcgagcacg ccgacgagct ccacggcggt cccgacgagc gcgaccgtcc gccaccgcgc
     1201 ggtcgcgcgc cagagtgcga aggcgatgac gccgtagatc agggccgcga cggcggagag
     1261 ccagtagggc agcggcgcct cggaggcctt cgtggtgagc tgcacactcg agcgccccgc
     1321 ggcggcgagg aagaggatcg cgtaggtgac gaggagcgca aggcggggca ggggcttcat
     1381 accgcgaccc tagcccgcga gcaggatggt gttaaggcgc cacgcgacga cggcgagcgc
     1441 cgcgccgacg atcatcgcgg cggcaccgtc cgcctggacc atctggcgcg ggctgagggc
     1501 gggacgatcg ggatcatggg cctcgccggg cgcgggaggt gcgccgagtc ggctgatgcc
     1561 gagaagcccg agcacggcga ccgcggcgac gaggtagccc acaagcatga tcatgggcgg
     1621 gtgggtgacg ccggcgccga ggagagcgcc gatcgcgacg atctcgatgc ccgtcgcccc
     1681 gcgcgccacc caggtggcga cgatgaggag gcgcgcgaac cgcgcggtgt tctgtgccgc
     1741 cgcacccgcc gtcggaccaa tccgtgtcgc ggcgacgtag gccaccgcgc ccgccaccag
     1801 gaccgcgacc atcgccagca ggaccgccgg gtacaccgcg tcgctgaacg ccctcatcgc
     1861 gtcgtccgct ccctagcccc tgggcaccaa ttccctcgcg ggccctagcc ccgctgctcc
     1921 tgcacgcgca tgggcagccg ggtcgcttcc tcgccgtcga tcgtgatgac ccaggagaac
     1981 atccccggcc cctcgaaccg catgccgtcg atggtgaacg cgaagcacgc gatttgctcg
     2041 tcgccgtcca cgagttgcgg cggacggccg agcgtgaact cgccgcccat cgcggtgacg
     2101 ggaacgggcc gcccctcggc gtccgcgcgc atcccgatag ggtggtcgac gccgtcctcg
     2161 gtcaccaggc gaatctcgaa cttgtgcgcg gtgttggtgt cggtgaaggg aacgtgcacg
     2221 gtcgcggcga ccgtgaggcg ccggtggatg accgggaatg cgcgcgcgaa gatcgtgttc
     2281 cagcccccgc ccagcgcata gatcttgttg ttgatgacat cggcggaatc cgccaggaac
     2341 gcatcgactc tcacggtgac cctttcgctc gtgcctcagc ctaccggggc gcatagggtg
     2401 tgagcgtggc cggaacgatc aatcgagacg acatcgcggc ggtgcgcgag aaggcgccgc
     2461 tcgaggacat cgttcgcgag cgcgtcgcgc tcaagcaggc cggggtcgga tccctcaagg
     2521 gcctgtgccc cttccacgac gagaagtcgc cgtcgttcca cgttcgcccc atgcagggcc
     2581 actggcattg cttcgggtgc ggcgagggcg gggacgtcat cgccttcatc atgaaggccg
     2641 acggattgcc cttcgccgac gccgtggagt acctggcggg gcgcgccggg gtgacgctcc
     2701 ggtacgagtc gggcggggcg cgtggcgcgt cggacggcgg gggcggacgc aagcgcatgc
     2761 tcgaagcgca ccgcgtcgcg gccgagttct tcgtcgggca gttggtgacg ccagaggcga
     2821 agacgggccg cgacttcctc acggggcgcg ggttcggcaa ggaggcggcc gcgcagttcg
     2881 gggtcgggta cgcgcccaag ggttggtccg cgctcctgga ccacctgcgc ggcaaggggt
     2941 tcaccgagcc cgagatcgag gcctccggcc tggtctcgcg gggccagagc gcgtcgggcc
     3001 agagcgcccg tctctacgac cgcttccgcg gccggctcgt ctggcccatc cgcgccgtga
     3061 cgggagaggt catcggattc ggcgcccgca agctctacga ggacgacgag ggccccaagt
     3121 acctcaacac gcccgagacc gcgctgtaca agaagtcgca ggtgctctac gggctcgacc
     3181 tcgccaagaa ggacatcgcc tccacgcacc gtgtcgtcgt cgtggagggc tataccgacg
     3241 tcatggcgtg ccacctgtcc ggggtgacga cggcggtcgc gacctgcggg acggcgttcg
     3301 gcgaggagca cgccaaggtg ctccggcggc tcctcggcgg cgacgcgggc gcgccgacca
     3361 aggtgggcgg ctcgcgcgga gaggtgatct tcaccttcga cgccgatgac gcgggccaga
     3421 acgccgcgct caaggccttc cagctcgacg cgaccttcct gtcccagacg ttcgtcgccg
     3481 tcgggcccga cggcctggac ccgtgcgacc tgcgcctgag ggacggcgac gtggccgtcg
     3541 cggcgctcgt cgaggcgcgg gtcccgctgt atgagttcgt catcaagtcg gcgctcgcgg
     3601 gcaacgacct cgagacgccc gaggggcgcg tgacggcact gcgcgccgcg gcccccgtcg
     3661 tggccgggat tcgcgacgcg gccatcggcc ccgagtatgc gcggcgcctt tccggatggc
     3721 tcgggatgga acccgccgag gtcatgcgcg ccgtgacgac cgcggccaag gcggcccggg
     3781 gcgcggcgca ggggcgcgtc gacgtcacgg ccgccgttgc cgtcggcccg gaccccaagg
     3841 acccggtcgt tcgcgctgag cgcctcgcgc tcgaggtgct cctccagatg ccggaaaagg
     3901 tggagcccgc ctgggcggcg ggcatcgggc cggagacgtt cacggtgccg cagtaccgcg
     3961 cggtgctcga cgggatcgcc gccgcgggag gcatcgccac cgcgggcaag gactgggcga
     4021 accgcgttgc ggacgaggtg ggggaggtgc tcgccccgct cgtgtccgaa ctcatcgtct
     4081 cgcccgtgcc ccaggatcgg ccgtcggaga tggcccggta cgtcaagagc gtgttgtccc
     4141 gcgtgcagga cctcagcctc acccggcgga tcggcgaggt ccgcgggcgg atgcaacgca
     4201 tcggcgaggg caatccgggt taccaggagg catttgccga actcgtcgcc ctggagggtg
     4261 agcgtcgggg gcatcgggag gagtaggcgc cgacccggcg cgtggcgccg aatcggttaa
     4321 ccagttgcgg acggggagtt ctcatgaggc gctcaacgtg gttcatcgtc gcggcatcgg
     4381 gtttcgtttt ggcctcggtg g
//
LOCUS       DASVKP010000182         7428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_388422, whole genome shotgun sequence.
ACCESSION   DASVKP010000182 DASVKP010000000
VERSION     DASVKP010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388422"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VF362_03730"
     CDS             complement(<1..430)
                     /locus_tag="VF362_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEX7590069.1"
                     /translation="MSVTAPRGFRASGVTAGLKKSGKSDVALVVNDGPLHVAAGVFTS
                     NRVVAAPVLWSRQAIEDGRIDAVILNSGGANACTGPEGFGDAHATAEHTAAALRAAGM
                     DVSSGDVAVCSTGLIGVRLDMGALLPGATAAVGALSISDGD"
     gene            complement(516..1565)
                     /gene="argC"
                     /locus_tag="VF362_03735"
     CDS             complement(516..1565)
                     /gene="argC"
                     /locus_tag="VF362_03735"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX7590070.1"
                     /translation="MRMTITVAVAGASGYVGGEVLRVFGAHPDVEFGALTAAASAGDT
                     LGAHHQHLRSLAHRTLVATTPEALAGHDVVVLALPHGTSAAIAAALPPETLVIDCGAD
                     HRLASAEAWAEYYGGDHAGTWPYGLPELVTGALRQRSALVGARRIAVPGCNVTAVTLG
                     IQPGIAAGLVDACDLVAVLAVGYSGAGKALKPHLLASEGLGAAVPYAVAGIHRHIPEI
                     IQNLVAAGAADVRISFTPVLVPMSRGVLATVTAPLAHGATAASVREAWVSAYVDEPFV
                     EVLPGGQWPSTASTVGSNAVLIGVGVDAKAGRVVTVTALDNLVKGTAGGALQSMNIAL
                     GLPETTGLTTTGVAP"
     gene            1696..2418
                     /locus_tag="VF362_03740"
     CDS             1696..2418
                     /locus_tag="VF362_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590071.1"
                     /translation="MMNGTIARAVLGAAAIALSLAGCTGGGGPSGSPSASATATAATS
                     STATTTTTTTTTTTTTTTTAAGGCPAWNDAAAAGGLASVDFNRFAGICIGMDYPSASA
                     AAGYDIAGKPACPWVADIVADDANGFYVTALSPVESAGSQITFFRMAWLNDPATASSY
                     EMPATAEGITIGSTLAQLQAAYPSATHYAIDDMSRGPRDEWVVLGANGNEYIFDVTAG
                     LVSELNWGQLQPTGIQGEYCAR"
     gene            2472..3521
                     /locus_tag="VF362_03745"
     CDS             2472..3521
                     /locus_tag="VF362_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7590072.1"
                     /translation="MKVLFLGGTGVISSECAALVTARGHQLTLITRGRSQTAPPPPGA
                     RLLHADASDPLALRAALSEGAGGERFDAVVQFVGYEPGQVADDVITFAPRARHYVFVS
                     TAAAYRNFDRFVLVTEETEQANPHWEYARKKAESERVLRTYAADAGLPFTIVRPAHTY
                     GASRIPGYVGNSRHPWTLVDRMRRGADIIIPGDGTSVWTLTHARDVAVGLVGLLGNNR
                     AFGEAVNIMGEEALTWNGIHATIAAAAGLTVPQFAAISLHVPSDAIVAADPTLAGGVY
                     GDKMHGAIFDTSKIRSLVPDFAPAITFADGVREALAWFEANPERQTVDAEANAMLDRL
                     GRAYRTALRDVTPSA"
     gene            3664..4728
                     /locus_tag="VF362_03750"
     CDS             3664..4728
                     /locus_tag="VF362_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7590073.1"
                     /translation="MRVLFIGGTGLISSGVSPLVVERGHDLTVLNRGTSPKASAPQGA
                     RVITADARDPEVLRAAVRADVAAHGNYDAVVQWICFTPDHLAQDIQTFAGLAGQYVFI
                     SSASAYETPPTRPITTEATPLSNPFWQYSRDKAACEALLREAGAASAFPFTIVRPSHT
                     YGYSDIPWGINSWTRPWTIIDRIRSGKPFIVHGDGTTLWTLTDHRDFAVGLVGLLGNP
                     GAIGEDFHITSDDVLTWNQIHSIVADAAGILPEALADQAIYIPSALLARFDAEAFEGP
                     LMGDKANAAIFDTSKLRALVPDFATRHYLRDSIGESMAWFRADASRRELDEDANELWD
                     RVILAYRDAIDGIFPEDARV"
     gene            complement(4733..5866)
                     /gene="tal"
                     /locus_tag="VF362_03755"
     CDS             complement(4733..5866)
                     /gene="tal"
                     /locus_tag="VF362_03755"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX7590074.1"
                     /translation="MNANHVKFVAPRASATPLAALAAAGVSIWLDDLSRGRLTSGSLA
                     RDVAAGRIVGVTTNPSIFAASIGQSEDYDDQIDDLRSTGVSVGEAVRMMTTTDVREAC
                     DVLRPAYDATGRVDGRVSIEVNPMLAREPEATIAEARQLWWLVDRPNAMIKIPATREG
                     LTAIRAAIGVGISVNVTLIFSLDRYSEVVDAYLGGLEDARAAGHGLATIQSVASFFIS
                     RIDTEVDKRLDAARPGSALRGTAAVASARLAYEHHRAVLASPRWKALAAAGATPQRPL
                     WASTSVKDLAFPDTKYVVDLVAPGTVNTMPPATLEAVADHGVIPADSVTGTYDGARAA
                     LAALAAAGVDMGDVTTVLEDEGVQKFADAWRTLEEGIASALTA"
     gene            complement(5902..6903)
                     /locus_tag="VF362_03760"
     CDS             complement(5902..6903)
                     /locus_tag="VF362_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEX7590075.1"
                     /translation="MTVVDNAIYVEGRRVASPKKLDQTFEALRDLGGTAWLGLREPSA
                     GELGVVAAELGLHDLAVEDALKGQQRSKLETFGDTMAVVVRSARYHDDTETVEFSDVH
                     VFVGAAYVVTVCQAVSPGLAVVRRRMEGLPSLLAMGPLAILYGILDEIVDGYLPVVAG
                     LENDIDEIEDQIFGESGSGSLSQRIYELHREVIGFQRAVHPLAAVLQDLSKGAARQGA
                     DVELTRRLRDVHDHILRVDDRIDGFRSLLDNALRAHTTIVAQQQTEASFSQNEEIKRI
                     SAWAAILFAPSLVGTTYGMNFHHIPELGWVWGYPFALGLMAALSVSLFIVFKRLRWL"
BASE COUNT         1126 a         2688 c         2527 g         1087 t
ORIGIN      
        1 catcgccatc ggatatggag agcgcgccaa ccgcggccgt cgcgccgggc aggagcgcgc
       61 ccatgtcgag ccggacgccg atgagccccg tggagcacac cgcgacgtcg ccggaggaca
      121 cgtccatccc cgccgcgcgc aacgccgcgg ccgtgtgttc cgccgtcgcg tgcgcgtccc
      181 caaagccctc ggggccggtg cacgcgttcg cgcctcccga gttgaggatc acggcgtcga
      241 tccggccgtc ctcgatcgcc tgccgcgacc acaacacggg cgcggcgacc acgcggttgc
      301 tcgtgaagac gcccgccgcg acatggaggg gcccgtcgtt gacgacgagc gccacgtcgc
      361 tcttgccgga cttcttgagc cctgcggtga ccccggacgc ccgaaaccct cgcggtgccg
      421 tgacgctcat ggtgcactcc tctcgccgcg cgccgtcacg gcgcactcgt tccctccgcg
      481 cgccgtcacg gcgcactcgt tccctccgcg cgccgtcacg gcgcgacccc tgtcgtcgtc
      541 aggcccgtcg tctcgggaag ccccagcgcg atgttcatgg actggagcgc gccgcccgcc
      601 gtgcccttta cgaggttgtc gagcgcggtg accgtcacga ccctgcccgc cttcgcgtcg
      661 acgccgacgc cgatcaggac ggcgttcgat cccacggtcg aggccgtgga cggccactgc
      721 cccccgggga gcacctcgac gaacggctcg tcgacgtagg cggataccca cgcctccctg
      781 acgctcgcgg ccgtcgcacc gtgggcgagc ggcgcggtga ccgtcgccaa gacgcccctg
      841 gacatcggga cgagcacggg cgtgaaactg atgcgcacgt ccgcggcgcc cgccgccacg
      901 aggttctgga tgatctcggg gatgtgccga tggattcccg ccacggcgta cgggacggcc
      961 gcgccgagcc cctcggaggc aagcaggtgc ggcttgaggg ccttgcccgc gccggagtag
     1021 ccgacggcga ggacggcgac gaggtcgcac gcgtcgacga gtccggccgc gatcccgggc
     1081 tggatcccca gggtgacggc ggtcacgttg cagccgggga cggcgatccg cctcgcgccg
     1141 acgagcgcgg agcgctggcg aagcgccccc gtcacgagtt cggggagccc gtacggccac
     1201 gttcccgcat ggtcgccgcc gtagtactcg gcccatgcct cggcgctcgc gagtctgtgg
     1261 tcggcgccgc aatcgatcac gagcgtctcc ggcggcaggg cggcggcgat cgcggcggag
     1321 gtcccgtggg gaagggcaag gacgacgacg tcgtggcccg cgagcgcctc gggagtcgtc
     1381 gcgacgaggg tccgatgcgc gagcgatcgc aggtgctggt ggtgcgcgcc gagcgtatcc
     1441 cccgcgctcg cggcggccgt gagcgcgccg aactcgacgt cggggtgtgc gccgaagacg
     1501 cgcagcacct cgccgccaac gtagccggac gcgccggcga ccgcgaccgt gatcgtcatg
     1561 cgcataacaa tacagatgaa tgcatgttcc tgcaacgtgg cggcgagatg aaaccgagac
     1621 gtcaattccc cccaatcgtc atccggcccc ggcacaatgg tgcacgggtg aggactagcg
     1681 tctatgcggg aggcaatgat gaacggaact attgctcgcg cggtactcgg tgcggcggcc
     1741 attgccttgt cgctcgcggg ttgcacgggg ggcggcggcc cgagcgggag cccgagcgcc
     1801 tccgcgacgg ccacggccgc aacgtcgtcg acagcaacaa cgacgacgac gacaacgacg
     1861 acgaccacga ccaccaccac gacggccgct ggcggttgcc ccgcgtggaa cgacgccgcc
     1921 gcggcgggtg gcctcgcatc ggtcgacttc aatcgattcg cggggatctg cattgggatg
     1981 gactacccct cggcctcggc cgcggcgggg tacgacatcg cgggcaaacc cgcgtgcccg
     2041 tgggtcgccg acatcgtcgc cgacgacgcc aacggcttct acgtcaccgc gctctccccc
     2101 gtcgagtcgg cgggatcgca gatcaccttc ttccggatgg cgtggctcaa cgacccggcc
     2161 accgcctcgt cgtacgagat gcccgccacc gcggagggga tcaccatcgg tagcaccctg
     2221 gcccaactcc aggcggccta cccgagcgcg acgcactacg cgatcgacga catgtcccgc
     2281 ggcccccgcg acgagtgggt ggtgctcgga gcgaacggaa acgagtacat cttcgacgtg
     2341 accgccggac tcgtctcgga actcaactgg ggccagctcc agccgacggg aatccagggt
     2401 gagtactgcg cgcgctaggg gcgagatgag ccgaacccgg cggcgaaccg ctccggggga
     2461 acggtagcga gatgaaggtc ctgttcctgg gcggaacggg agtgatctcg tccgagtgcg
     2521 ccgcgctcgt caccgcgcgg gggcaccaac tcaccctcat cacgcgcggg cgctcccaga
     2581 cggctcctcc cccgcccggc gctcggctcc tccacgcgga cgcgagcgac cccctcgccc
     2641 tgcgcgccgc cctgagcgag ggcgcgggcg gcgagcgatt cgacgccgtc gtccagttcg
     2701 tgggctacga gcccggacag gtcgccgacg acgtcatcac cttcgcgcca cgggcccgtc
     2761 actatgtgtt cgtctccacc gcggcggcgt accgcaactt cgatcgcttc gtgctggtca
     2821 ctgaggagac ggagcaggcg aacccccact gggagtacgc gcgcaagaag gccgagagcg
     2881 agcgcgtcct tcgcacctac gcggccgacg ccggccttcc cttcactatc gtgcgaccgg
     2941 cacacaccta cggggcatcg cggatccccg gctacgtcgg caactcccgc cacccgtgga
     3001 ccctcgtcga ccggatgcgg cgcggcgcgg acatcatcat ccccggtgac ggcacctccg
     3061 tgtggacgct gacccacgca cgcgacgtcg cggtcgggct cgtaggcctc ctgggcaaca
     3121 accgggcatt cggggaggcc gtcaacatca tgggagagga ggccctcacc tggaacggca
     3181 tccacgccac gatcgcagcg gccgcgggcc tcaccgtgcc ccagttcgcg gcgatttccc
     3241 ttcacgttcc ctccgacgcg atcgtcgccg cggatcccac actggcgggc ggcgtctatg
     3301 gcgacaagat gcacggcgcc atcttcgaca cgagcaagat ccgctctctc gtccccgact
     3361 tcgccccagc catcacgttc gccgatggcg tcagggaggc gctcgcgtgg ttcgaggcga
     3421 acccggaacg ccagacggtc gacgccgagg cgaacgcgat gctcgatcgc cttggccgcg
     3481 cctaccgaac cgccctgcgg gacgtcaccc cgagcgcgta ggccggcaag cccgtcacct
     3541 tgccacagcg tcgctggcta gcgtcgcgcc cggcgtcgcc cgtgccgggg gccgcgaaac
     3601 cgccctgggg tagcctggcg gcggcgagcc atttcgggtt caccggtagc ccgcggaggt
     3661 cccatgcgcg tcctgttcat cggcggaacc ggcctcatct cgtccggcgt ctctccgctc
     3721 gtcgtcgagc ggggccatga tctcaccgtc ctcaaccgcg ggacctcgcc caaggcctcc
     3781 gcgccccagg gcgcgcgggt catcacggcg gacgcccgcg accccgaggt gttgcgcgcg
     3841 gcggtacggg cggacgtggc cgcccacggg aactacgacg cggtcgtcca gtggatctgc
     3901 ttcacccccg atcacctcgc gcaggacatc cagaccttcg ccggactcgc gggtcagtac
     3961 gtcttcatct cctcggcgag cgcgtacgag accccgccga cgcgccccat cacgaccgag
     4021 gccacgcccc tgtcgaatcc cttctggcag tacagcaggg acaaggccgc gtgcgaggcg
     4081 ttgctgcggg aggcgggggc cgcctccgca ttcccgttca ccatcgttcg gccgtcccac
     4141 acctacggct actcggacat cccgtgggga atcaactcgt ggacgcggcc gtggacgatc
     4201 atcgaccgca tccgcagcgg caagccgttc atcgtccatg gcgacggcac gaccctgtgg
     4261 accctcaccg accaccggga cttcgcggtc ggcctcgtcg ggctcctcgg caaccccggc
     4321 gctatcggcg aggacttcca catcacgtcg gacgacgttc tcacgtggaa tcagattcac
     4381 tcgatcgtcg ccgacgcggc gggcatcctc cccgaggcgc tcgcggacca ggccatctac
     4441 attccctcgg cgctcctcgc gcgcttcgac gccgaggcgt tcgagggccc gctcatgggc
     4501 gacaaggcaa acgcggcgat cttcgacacg tcgaagctca gggcgctcgt gcccgacttc
     4561 gcgacgcgcc actacctcag ggactccatc ggcgaatcga tggcgtggtt cagggccgac
     4621 gcctcgcgac gcgagctcga cgaggacgcc aacgaactct gggatcgggt catcctcgca
     4681 taccgcgacg cgattgacgg catcttcccc gaggacgccc gggtttaggc ggtcaggcgg
     4741 tcagcgcgga cgcgatccct tcttccaggg tgcgccacgc gtccgcgaac ttctggaccc
     4801 cctcgtcctc cagaaccgtc gtcacgtcgc ccatgtcgac gcccgccgcg gccagcgcgg
     4861 cgagcgccgc gcgcgcgccg tcataggtgc ccgtgaccga gtccgcgggg atgacgccgt
     4921 gatcggcgac ggcctcgagg gtcgcgggcg gcatcgtgtt gacggtgccc ggggcgacga
     4981 gatcgacgac gtacttcgtg tcggggaacg cgaggtcctt gaccgaagtt gaggcccaga
     5041 gcgggcgctg cggtgtcgca cctgcggcgg cgagcgcctt ccatcgcggg gaggcgagca
     5101 cggcccggtg atgctcatag gcgagtcggg cgctcgcgac cgccgccgtg ccccgcaggg
     5161 cggaaccggg acgcgcggcg tcgagccgct tgtcgacctc ggtgtcgatg cggctgatga
     5221 agaacgacgc gaccgactgg atggtggcga ggccgtgccc ggccgcccgg gcgtcctcga
     5281 ggccgccgag gtacgcgtcg accacctcgg agtaccggtc cagggagaag atcagcgtga
     5341 cgttgacgga gattcccacc ccgatcgccg cgcggatcgc cgtgaggccc tcgcgcgtcg
     5401 ccggaatctt gatcatcgcg ttgggccgat cgacgagcca ccagagttgc ctcgcctccg
     5461 cgatcgtcgc ctccggctcg cgcgcgagca tggggttcac ctcgatcgag acgcgtccgt
     5521 cgacgcggcc cgtcgcgtcg taggccgggc ggagcacgtc gcacgcctcg cggacgtcgg
     5581 tcgtcgtcat catccggacg gcctcgccca cggacactcc ggtcgagcgg aggtcatcga
     5641 tctggtcgtc gtagtcctcg gactggccga tggaggcggc gaagatgctc gggttggtcg
     5701 tcacgccgac gatgcgcccc gcggcgacgt cgcgggcgag ggagccggag gtcaggcgcc
     5761 cgcggctgag gtcatcgagc cagatggaga cgcccgcggc ggcgagcgcg gcgagcggcg
     5821 tggcgctggc cctcggcgcc acaaacttga cgtgattggc gttcatctgc gtccctccca
     5881 catctcggtc tctccctggc cctagagcca ccgcaatcgc ttgaagacaa tgaagagact
     5941 cacgctcagc gccgccatga ggccgagcgc gaacgggtag ccccagaccc agccgagctc
     6001 ggggatgtga tggaagttca tgccgtaggt ggtgccgacg agcgaggggg cgaacaggat
     6061 cgcggcccac gcggagatcc gcttgatctc ctcgttctgg gagaagctcg cctcggtctg
     6121 ctgctgcgcg acgatcgtcg tgtgcgcgcg cagggcattg tcgagaaggg agcgaaaccc
     6181 gtcgatgcgg tcgtccaccc gcaggatgtg gtcgtggacg tcgcggagcc gcctcgtgag
     6241 ttccacgtcg gcgccctggc gggcggcccc cttgctcagg tcctggagta ccgcggcgag
     6301 cgggtggacg gcgcgctgga agccgatgac ctcccggtgg agttcgtaga tgcgctggga
     6361 gagcgaacca cttccgctct ccccgaagat ctgatcctcg atctcgtcga tgtcgttctc
     6421 caggcccgcg acgacgggga gatagccgtc cacgatctcg tcgaggatcc cgtaaaggat
     6481 ggcgagcggc cccatcgcaa ggagcgaggg aagtccctcc atccggcgcc ggaccaccgc
     6541 caggccgggc gacaccgcct gacacaccgt gacgacgtac gcggcgccga cgaacacgtg
     6601 gacgtcgctg aactccaccg tctccgtgtc gtcgtggtat ctggcggagc gaacgacgac
     6661 ggccatcgtg tcgccgaagg tctccagctt ggagcgctgc tggcccttga gcgcgtcctc
     6721 gaccgcgaga tcgtggaggc ccagttcggc ggccacgacg cccagttccc ccgccgaggg
     6781 ttcgcgcagg ccgagccacg ccgtcccgcc aaggtcgcga agcgcctcga acgtctggtc
     6841 gagcttcttc ggagaggcca cacgacgccc ctcgacgtag atcgcgttgt cgacgaccgt
     6901 cacaatgccc cttccctgcg ccgcgcccgt tggggccccg cgccccggca tagggcgcca
     6961 gcaccatgct aggaccccgc cgctgcgcct cgtggcaagt cgggcccacc gtcgatcgct
     7021 gctcggcgac ttgacagtcc aggtggaggg acgctgtact tcgcgcgtcg aacggccgtt
     7081 gcccgtgtcg cggctaccct tgagtcaacc agggaggtga gggacgtgaa tcccggagat
     7141 agtcgcccgc atagtgatct gccctcccca cccgtcgacc atcgcttcgg agcgaagccg
     7201 tttccctccg tagctggcca gtcgccgtgc aggcggaccc tcgtcgtcga ggattcccgc
     7261 acctcgtggc gcgcctaggt gcgctccggg tccccttctg tcctgcgctc ccttcgggct
     7321 gggcgctgct gcgtttggag catgtagtga ccgagaacac gactggcgcc agcgcggtgc
     7381 tcgacagcgc ccacaccggc gacatcgagg gagcgttcgg cacgattc
//
LOCUS       DASVKP010000183         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1459445, whole genome shotgun sequence.
ACCESSION   DASVKP010000183 DASVKP010000000
VERSION     DASVKP010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459445"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..>2044
                     /gene="dxs"
                     /locus_tag="VF362_03765"
     CDS             241..>2044
                     /gene="dxs"
                     /locus_tag="VF362_03765"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX7590076.1"
                     /translation="MLESIGSPEDLASLDYDELRELAAEIRAYLVDSVSKSGGHLGPN
                     LGVVELTLAIHRVFESPRDSIVFDTGHQSYVHKLLTGRQDFSNLRKRGGIAGYPNRAE
                     SEHDIVENSHASTALSWASGIGRANTLMGRADRTVVAVIGDGALTGGMAWEALNQIAD
                     SDDRIVIIVNDNGRSYAPTIGGLARRLDDIRTNPRYEAVLGWGERTLRRKGPLGRLTF
                     WTIKRFKRGLRDVLTPQGLFGDLGIKSVGPVDGHDMRNLEHALKHARDYGAPVIVHVL
                     TEKGRGYHPAENHQEDKFHAVGRIHPETGLPVEPSRFGWTAVFADEILKAARARDDIV
                     AITAAMQAPVGLQPLANEMPERVIDVGIAEQHAATVAAGLAFAGFHPVVAVYATFLNR
                     AFDQILMDVALHKAGVTFMLDRAGITGDDGPSHNGMWDMALLRSVPGIRIAAPRDEAT
                     LRSAFREAIEVSDAPTVVRYPKDELGDGLPALERRGKALSRGGIDVLAEHGKDPRVVV
                     FGIGAMASTAIEVAQRIAAEGFSCRAATATWVIPVPEGLSDEVRDADAVITIEDGLVE
                     GGIGEAWANAANSAGNRAAWTHFGIPRAFLAHATR"
BASE COUNT          317 a          708 c          724 g          295 t
ORIGIN      
        1 cgggccgtcg tgcgaatcga cacgcccggc gaggcgcagt acttccgcca tggtgggatc
       61 cttcagtacg tgttgcggtc gctcgcgggg agctagcggc gccgcgcgct ggcggtggtt
      121 gagtgccgaa atctgcgcgc aatcggcaca ggggcgccgc gacgccgcgg gcggcgcgac
      181 ggtcgcgggg cccggggcgc cgtagagtgg gcgggtcggt tcagagggag tgaggccgca
      241 gtgctggagt ccatcggctc gcccgaggac ctcgcgtccc tcgactacga cgaactgcgc
      301 gaacttgccg cggagatccg ggcgtacctg gtggactccg tctccaagtc cggcggccac
      361 ctgggaccca acctcggcgt cgtcgaactc acgctcgcga ttcaccgcgt cttcgagtcg
      421 ccgcgggact ccatcgtctt cgacaccggc caccagagtt acgtccacaa gctcctgacg
      481 gggcgtcagg acttttcgaa cctgcgcaag cgcgggggga ttgccggcta cccgaatcgc
      541 gccgaatccg aacacgacat cgtcgagaac tcccacgcct cgaccgcgct ctcgtgggcg
      601 tcgggcatcg gtagggcgaa cacgctgatg gggcgcgcgg atcgcacggt cgtcgcggtg
      661 atcggcgacg gcgccctcac gggcgggatg gcctgggagg ccctcaacca gatcgcggac
      721 agcgacgacc ggatcgtcat catcgtcaac gacaacggcc gctcctatgc gccgacgatc
      781 ggggggctcg cgcgcaggct cgacgacatc cgcacgaacc cgcgctacga ggccgtgctc
      841 gggtgggggg agcgcacgct ccgtcgcaag ggccccctcg ggcgcctgac gttctggact
      901 atcaagcgct tcaagcgggg cctcagggac gtcctcacgc cccaggggct ctttggggac
      961 ctgggcatca agtccgtcgg cccggtcgac ggccacgaca tgcgaaacct cgagcatgcg
     1021 ctcaagcacg ccagggacta tggggccccg gtaatcgtgc acgtgctgac cgagaagggc
     1081 cgcggctatc accccgccga gaatcaccag gaggacaagt tccacgcggt gggtcggatt
     1141 cacccggaga cgggcctgcc cgtcgagccc tctcggttcg gctggaccgc tgtgttcgcc
     1201 gacgagatcc tcaaggcggc ccgcgcccgc gatgacatcg tcgcgatcac ggccgcgatg
     1261 caggccccgg tcggcctcca gccgctcgcg aacgagatgc ccgagcgggt catcgacgtc
     1321 ggcatcgccg aacagcacgc cgcgaccgtc gccgcgggcc tggcgttcgc cgggtttcac
     1381 cccgtggtcg cggtctacgc cacgttcctc aaccgcgcgt tcgaccagat cctcatggac
     1441 gtggcactcc acaaggccgg ggtcacgttc atgctcgacc gcgctggcat cacgggagac
     1501 gacggcccga gccacaacgg gatgtgggac atggccctcc tgcgatcggt gcctggcatt
     1561 cgtatcgcgg cgcccaggga cgaggcgacg ctgcgctcgg ccttccggga ggcgatcgag
     1621 gtgtccgatg ccccgacggt ggtgcgctac cccaaggacg aactgggcga tgggcttccg
     1681 gccctcgagc ggcggggcaa ggccctgtct cgcggcggga tcgacgtgct cgccgagcat
     1741 ggaaaggacc cccgcgtcgt cgtcttcgga atcggcgcga tggcctcgac ggcgatcgag
     1801 gtcgcgcaga ggatcgccgc ggaggggttc tcgtgccgcg cggcgaccgc gacgtgggtg
     1861 ataccggttc ccgaaggcct ctccgacgag gtgcgcgacg ccgacgccgt gatcaccatc
     1921 gaggacgggc tggtcgaggg gggcatcggc gaggcctggg ccaacgcggc gaactcggct
     1981 ggcaaccgcg ccgcatggac ccacttcggc atcccacgcg ccttcctcgc ccacgcgacg
     2041 cggg
//
LOCUS       DASVKP010000184         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049 k141_58241,
            whole genome shotgun sequence.
ACCESSION   DASVKP010000184 DASVKP010000000
VERSION     DASVKP010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58241"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1978)
                     /locus_tag="VF362_03770"
     CDS             complement(<1..1978)
                     /locus_tag="VF362_03770"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX7590077.1"
                     /translation="MTGSHAGSETTPRPEAAASSTPSAPSTPSWPPAPGAAPAPGAAP
                     APASSPAPALSPRRPDVPPPNALVDPIAPQVAPEATPDTRPEIPVPVDQAIADSTDAA
                     TQPVATAQAPTASYLQDLGDSDRALPDVREGSVRLRGSAALVVANMEKSLEVPTATSV
                     RSIPVKLLMDNRVVVNNHLERTRGGKVSFTHMIAYALIEALVELPRMNDHYALEDDKP
                     VVVTPPHINMGIAIDVSKPDGTRQLFVPSIKQAEDLDFAKFWVAYDDVIRRARGGTLQ
                     AADFQDTTISLTNPGTIGTEHSVPRLMSGQGAIIGVGAMDYPAEYQGASSETIAKLGI
                     SKVMALTSTYDQRVIQGAQSGEFLGLVHTKLLGHDGFYDRVFRSLRIPYEPMRWIQDG
                     ATSPEAEAIKPAHIAELIHAYRSRGHLMADVDPLSSRPRKHPDLDIQTHGLTLWDLDR
                     VYATAGFAGRERQRMREVLSRLRDAYCRTTGVEYMHITDRAQRAWMQERLEGGYRKSS
                     RDEHMRILRRLNAAEAFEAFLQTKYVGQKRFSLEGGESLIPLLDAALSSAAASQIDEV
                     CIGMAHRGRLNVLANLAGKSYAQIFKEFEGSADPRSVHGSGDVKYHLGTEGLFTSDSG
                     QQTKVYLAANPSHLEAVDPELEGIVRAKQDRIDLG"
BASE COUNT          295 a          679 c          868 g          378 t
ORIGIN      
        1 cgccgaggtc gatacggtcc tgcttggcac gcacgatgcc ctcgagctcc gggtcgacgg
       61 cttcgaggtg ggacgggttg gccgcgaggt agaccttggt ctgctggccg ctgtcggagg
      121 tgaagagccc ttcggtgccg aggtgatact tgacgtcgcc ggagccgtgc acgctgcgcg
      181 ggtcggcgct tccctcgaac tccttgaaga tctgcgcgta gctcttgccc gcgaggttgg
      241 cgagcacgtt gaggcggccg cggtgggcca tgccgatgca cacctcgtcg atctgggagg
      301 cggccgcgga ggagagcgcg gcgtcgagga gcgggatcag ggactcgccg ccctcaagcg
      361 agaagcgctt ctgcccgacg tacttggtct ggaggaacgc ctcgaaggcc tcggccgcat
      421 tgaggcggcg caggatcctc atgtgctcgt cgcgggacga cttgcggtag cccccctcga
      481 gccgctcctg catccacgcg cgctgcgcgc ggtcggtgat gtgcatgtac tcgacgcccg
      541 tggtccggca gtaggcgtcg cgcaggcggc tcaggacctc gcgcatgcgc tgccgctcgc
      601 gcccggcgaa gcccgcggtc gcgtagacgc gatcaaggtc ccacagggtg aggccgtggg
      661 tttggatgtc gagatccggg tgcttgcggg ggcgcgacga gaggggatcg acgtccgcca
      721 tgaggtggcc gcgggagcgg taggcgtgga tgagttcggc gatgtgggcg ggcttgatcg
      781 cctcggcctc cggcgacgtg gcgccgtcct ggatccaccg catgggctcg tagggaatcc
      841 ggagcgagcg gaagacgcgg tcgtagaacc cgtcgtgccc caggagtttg gtgtggacga
      901 ggccgaggaa ctcgcccgat tgggccccct ggatcaccct ctggtcgtac gtcgaggtga
      961 gcgccatgac cttggagatt cccagcttcg cgatcgtctc ggaggatgct ccctggtatt
     1021 cggccgggta gtccatggcg ccgacgccga tgatcgctcc ttgtcccgac atcagccgag
     1081 gcacggagtg ttcggtgccg atcgttccgg ggttggtcag cgagatggtg gtgtcctgga
     1141 agtccgccgc ctgcagagtg ccgccccgag cgcgacggat cacgtcgtcg tacgccaccc
     1201 agaacttcgc gaagtcgaga tcctccgcct gcttgatgct cggcacgaag agttggcgcg
     1261 tgccgtcggg cttggacacg tcgatcgcga tccccatgtt gatgtgggga ggggtgacga
     1321 cgacgggctt gtcatcctcg agggcgtaat ggtcgttcat ccggggaagt tcgacgagcg
     1381 cctcgatgag ggcgtacgca atcatgtggg tgaaggacac cttgccgccg cgcgtgcgct
     1441 ccaggtggtt gttcaccacg acgcggttgt ccatgagcag cttcacgggg atgcttctga
     1501 cggacgtcgc cgtgggcacc tcgaggctct tctccatgtt cgccacgacg agcgcggcgg
     1561 agccgcggag gcgcacggag ccctcgcgca cgtcggggag ggcgcggtcc gagtccccga
     1621 ggtcctggag gtacgacgcg gtcggggcct gcgcggtcgc gaccggctgc gtggccgcgt
     1681 cggtcgagtc ggcgatggcc tggtcgacgg ggacggggat ctccggccgg gtgtccggcg
     1741 tcgcctcggg cgcgacctgc ggcgcgatgg gatcgacgag cgcgttcggc ggcggcacgt
     1801 cgggacgtcg gggcgagagc gcgggggcgg gggacgacgc gggtgccggg gctgcgccgg
     1861 gtgccggggc tgcgccgggc gcgggcggcc aggacggcgt cgaaggggcg gatggcgtcg
     1921 aagaggcggc ggcctcgggc cgaggagtcg tctcggagcc agcgtgtgag cccgtcagga
     1981 gcgccgcgac cgacgagggg tcgatccctc gggcgacgga cgttcgtgct gactcggact
     2041 cggggctggg cgcgggcgtc tcggcggtca cttgtgggct actcgccttc ctggcgcgcg
     2101 tgcgtcgtga cggggatcgt gttctgcttc atcctaagcc agtcgggacc ttggtcgggg
     2161 gagccgccgt ggcccgctat tcctcctcgg actgcggcgg ccctatgctg accacagata
//
LOCUS       DASVKP010000185         3577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_884724, whole genome shotgun sequence.
ACCESSION   DASVKP010000185 DASVKP010000000
VERSION     DASVKP010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884724"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VF362_03775"
     CDS             <1..1086
                     /locus_tag="VF362_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX7590078.1"
                     /translation="AYYRWTHLMALTEVGAPAARFALPPATFHAWAAEQQSTYPHAMT
                     CLTTHDTKRSEDVRARLGVLSEFGQTWDEAVERMRVASEPYRPSELDGRMENLWWQTL
                     AGTYDDSGPLEWHRLSDYLVKAMREAKSFTTWTSSNERYEHAVLGFADLTLHDVAIGE
                     ILETWHRDTMASRRAATLGMKLLQLTIPGVADVYRGTETCDPSLVDPDNRRPVDFDTL
                     ALTLSRLDAGTRPRTLAEEKQWVTACALRVRRARRDAFIGEASGYQALASSSGHAVVF
                     ARTLSGDPVVITVATRMALELEHLGGWADATVQLPEGSLPEGSLPEGTRLKGTWRDAL
                     TGTTHAGGAAPLADILATYPVALLERA"
     gene            1089..2846
                     /gene="treZ"
                     /locus_tag="VF362_03780"
     CDS             1089..2846
                     /gene="treZ"
                     /locus_tag="VF362_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX7590079.1"
                     /translation="MQIGVWAPTAQSVVVHVVARAGTERFDFSARMDQCRAGWWIGPE
                     VAPGVDYAFSVDGAGPFPDPRSAAQPSGVHGPSRAFDAKAFAWTDGAWRGRDARGAVF
                     YELHVGTFTPDGTLDAATARLPGLAAIGIEMVELMPVAPFPGRHGWGYDGVSIFAVHD
                     ANGGPEALHRFVDAAHAAGLGVCLDVVYNHMGPDGNYLSRFGPYFTSAYETPWGWAVN
                     LDQDGSGEVRRYLADNALRWLEDFHVDALRLDAVHQIHDHSPHHFLAQLSDEVAELAT
                     RLNRPLSLIAESDLNDDQMVTPTREGGRGMTAQWDDDIHHALHAYLTGERQGYYVDFG
                     SAECLDKAYRDAFWHDGRFSPFRGKDWGRPVAPDRDRRDFVVFASDHDQVGNRALGDR
                     PSATLSPGAQATELAFVLLSPFTPMIFMGEEYGEKRPWMYFTDFEDPTLAEAVRKGRT
                     EEFSGHGWQEVYGGPVAVPNPQDPATARASVLDPESATGFPHDELRAWFAELIAARRL
                     TARRGAWAKHPVGVEEPAPRVLTLRGPVIVHANLSDRPIDVPAARLLATFGTVTVTPS
                     HTIVARDSLILLDASEGTR"
     gene            2843..>3577
                     /locus_tag="VF362_03785"
     CDS             2843..>3577
                     /locus_tag="VF362_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEX7590080.1"
                     /translation="MSGKHGWHHDPSDRLRVTAGFSLANFDPGATPGWRGNKARAVAE
                     MNRRGADLEALQEQLFARGKEGDRRSALLVIQGIDTAGKDGIVSHVVGCVNPMGVKIT
                     SFGVPTEQERKHAYLWRIRNALPGPGILGVFDRSHYEQVLVVRVHDLVPRETWERYYD
                     QLNAFEAKAAAAGTTVVKIALLVSPTEQLKRLEARLDDPGKYWKYTPKDLDERGYWNE
                     YQAAYQAIFDRCSPATAPWFAIPADRK"
BASE COUNT          547 a         1315 c         1216 g          499 t
ORIGIN      
        1 gcgtactacc gctggaccca cctgatggcg ctgaccgagg tgggggcgcc cgcggcgcgc
       61 ttcgcgctgc cccccgcgac cttccacgcg tgggccgcgg agcaacagtc cacctacccg
      121 cacgcgatga cgtgcctcac gacgcacgac accaagcgca gcgaggacgt cagggcgcgg
      181 ctcggcgtgc tgagcgagtt cgggcagacg tgggacgagg cggtggagcg gatgcgcgtc
      241 gcctccgagc cgtaccgccc gtcggaactc gatgggcgca tggagaacct ctggtggcag
      301 accctcgccg gaacgtatga cgactccggg ccgctggagt ggcaccgcct ctccgactac
      361 ctggtcaaag cgatgcgcga ggcgaagtcg ttcaccacct ggacgtcgtc caacgaaagg
      421 tacgagcacg ccgtgctcgg attcgcggac ctcacgctgc acgacgtcgc gatcggggag
      481 atcctcgaga cgtggcaccg ggacacgatg gcctctcggc gcgccgcgac cctggggatg
      541 aaactcctcc aactgaccat cccgggcgtc gccgacgtct accgcggcac ggagacgtgc
      601 gacccgagcc tcgttgaccc cgacaaccgg cgcccggtcg acttcgacac cctcgccctg
      661 acgctctccc gcctcgatgc ggggacccgc ccgcgaaccc tcgctgagga gaagcagtgg
      721 gtgacggcgt gcgcgctgcg cgtgcggcgc gcccggcgcg acgcgttcat cggcgaggcg
      781 tcgggctacc aagcgctcgc gtcgtcatcc ggccacgccg tcgtcttcgc gcggaccctc
      841 tcgggcgacc cggtggtgat cacggtcgcg acccggatgg cgctcgagtt ggagcacctg
      901 ggcggctggg cggacgcgac cgtccagcta cccgagggtt cgctgcctga gggttcgctg
      961 cctgagggga cgcggcttaa gggaacgtgg cgcgacgccc tgacggggac gacgcacgcg
     1021 ggcggggcgg cgcccctcgc cgacatcctg gcgacgtacc ccgtcgccct cctggaacgg
     1081 gcgtagccat gcagatcggc gtgtgggcac cgacggcgca gtccgtcgtg gtccacgtgg
     1141 tcgcccgggc ggggacggag cgtttcgact tttcggcacg catggaccag tgtcgcgcgg
     1201 ggtggtggat cgggcccgag gtcgcccccg gggtggacta cgccttctcc gtcgacggcg
     1261 cgggcccctt ccccgacccc aggagcgcgg cccagccctc gggcgtccac ggcccctccc
     1321 gcgccttcga cgcgaaggcc ttcgcctgga cggacggcgc gtggcgggga cgcgacgccc
     1381 gcggcgcggt cttctacgaa ctccacgtcg ggacgttcac gccggacggg accctcgacg
     1441 ccgccacggc ccgcctgcct ggcctcgcgg cgatcgggat cgagatggtc gagttgatgc
     1501 ccgtcgcgcc gttcccgggc cgccacggct ggggctacga cggcgtttcc atcttcgccg
     1561 tccacgacgc caatggaggg ccggaggccc tgcatcgctt cgtcgacgcc gcgcacgccg
     1621 cagggctcgg cgtctgcctc gatgtggtct acaaccacat ggggcccgac ggaaactacc
     1681 tctcgcggtt cggcccgtac ttcacgtcgg cctacgagac gccgtggggg tgggcggtca
     1741 acctggacca ggacggctcc ggcgaggtgc gccgctacct cgcggacaac gcgctgcgct
     1801 ggcttgagga cttccacgtg gacgcgctcc ggctcgacgc cgtccaccag atccacgacc
     1861 actcgccaca ccacttcctc gcccaactct ccgacgaggt cgccgaactc gcgactcgcc
     1921 tgaaccgccc gctctcgctc atcgccgagt ccgacctcaa cgacgaccag atggtcaccc
     1981 cgacgcggga gggcgggcgc ggcatgaccg cgcaatggga cgacgacatt caccacgcgc
     2041 tccacgcgta cctcacggga gagcgccagg gctactacgt cgacttcggg tccgcggagt
     2101 gcctcgacaa ggcctaccgc gacgcctttt ggcacgacgg caggttctcg ccgttccggg
     2161 ggaaggactg gggtcggccg gtcgcccccg accgcgaccg ccgcgacttt gtggtcttcg
     2221 cctcggacca cgaccaggtc ggcaaccgcg cgctcggcga ccgcccgtcg gccacgctct
     2281 cccccggcgc ccaggcgacc gaactcgcgt tcgtgctcct ctcgccgttc accccgatga
     2341 tcttcatggg cgaggagtac ggcgaaaagc ggccgtggat gtacttcacc gacttcgagg
     2401 acccgacgct cgcggaggcc gtccggaagg ggcgaaccga ggagttctcc gggcatggct
     2461 ggcaggaggt gtacggcggc ccggtcgcgg tcccgaaccc ccaggacccc gcgaccgcgc
     2521 gcgcgtccgt cctcgacccc gagtccgcga cgggtttccc ccacgacgaa ctgcgcgcct
     2581 ggttcgccga gctgatcgcc gcacgtcgcc tcacggcgcg gcgcggcgcg tgggccaagc
     2641 accccgtcgg cgtcgaggag cccgcgccga gggtgctcac gttgcgcggc cccgtgatcg
     2701 tccacgcgaa cctgtccgac cggccgatcg acgtgccagc cgcgcggctc ctcgcgacgt
     2761 tcggcacggt aaccgtgacc ccctcccaca caatcgtcgc gcgggatagc ctgattctcc
     2821 tagacgcatc ggaaggaacg agatgagcgg caagcacggg tggcatcacg atccgagtga
     2881 caggctccgc gtcacggcgg gcttctccct ggcgaacttc gatcctggcg cgacgcccgg
     2941 gtggcgcggg aacaaggcgc gcgcggtcgc ggagatgaac cggcgcggcg cggacctcga
     3001 ggcgctccaa gagcaactct tcgcgcgcgg caaagagggc gaccggcgca gcgccctcct
     3061 cgtaatccag ggcatcgaca ccgcgggcaa ggacgggatc gtctcccacg tcgtggggtg
     3121 cgtcaatccg atgggcgtta agatcaccag cttcggcgtg ccgaccgagc aggagcgaaa
     3181 gcatgcctac ctgtggcgca ttcgcaacgc gctcccgggc cccggcatcc tcggcgtatt
     3241 cgatcgctcg cactacgagc aggtgctggt cgtcagggtc catgacctcg tcccacgcga
     3301 gacctgggag aggtactacg accagctcaa cgcgttcgag gcgaaggcgg ccgccgcggg
     3361 caccaccgtg gtgaagatcg ccctcctcgt ctcgcccacc gaacaactga agcgcctcga
     3421 agcgcggctc gacgacccgg gcaagtactg gaagtacacg cccaaggacc tcgacgagcg
     3481 cggctactgg aacgagtatc aggccgccta ccaggcgatc ttcgaccgct gctcgcccgc
     3541 cacggcgccg tggttcgcca tccccgccga ccgcaag
//
LOCUS       DASVKP010000186         4954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_721316, whole genome shotgun sequence.
ACCESSION   DASVKP010000186 DASVKP010000000
VERSION     DASVKP010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721316"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..795)
                     /gene="priA"
                     /locus_tag="VF362_03790"
     CDS             complement(58..795)
                     /gene="priA"
                     /locus_tag="VF362_03790"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HEX7590081.1"
                     /translation="MNDAPRLQLLPAVDVAAGQAVRLVQGEAGSETSYGDPITAALDW
                     QAGGAEWIHLVDLDAAFGRGSNAVMLAEVVGQLDVKVEMSGGIRDDASLEQALATGCA
                     RVNLGTAALEDPDWTASAIARYGDRVAVGLDVRGTTLAARGWTKEGGDLWEVLARLDA
                     AGCSRYVVTDVTKDGTLRGPNLELLAQVCAATRVPVVASGGISSLEDLVALRSLVPLG
                     VEGAIVGKALYAGAFTLPEALDVAGRP"
     gene            complement(792..1445)
                     /gene="hisH"
                     /locus_tag="VF362_03795"
     CDS             complement(792..1445)
                     /gene="hisH"
                     /locus_tag="VF362_03795"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX7590082.1"
                     /translation="MAQPLVCVLDYGFGNVRSAVRAVEEVGATAILTADRQAAEGADG
                     LIVPGVGAFAAVLAGLRAVRGDQIVERRLAGGRPVLGICVGLQVMFEEGEEHGSTAPG
                     LAQWPGIVDRLDAPVVPNMGWSTVEAPEGSVLFCGVENERFYFVHSYAARSFPLNDEP
                     EGRLPKPLVTWSEAGTPAHHDRFVAAVENGPLSATQFHPEKSGPAGLALLKNWIGTL"
     gene            complement(1493..1972)
                     /locus_tag="VF362_03800"
     CDS             complement(1493..1972)
                     /locus_tag="VF362_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590083.1"
                     /translation="MTDELPGYLARDTVALLTPIQNGKVLAAICALNGAKCDVIEVPQ
                     GTLAVLADDSEGAGDRAAMAVTALHREIVLLAMERRGGQVTVTRWERGERGADVPPGL
                     ALDQAPGGVQRLMLGSATIEDLLAEDPSKVYRGRTGRFRAIWRLRRLAAAAKREQRG"
     gene            complement(2003..2602)
                     /gene="hisB"
                     /locus_tag="VF362_03805"
     CDS             complement(2003..2602)
                     /gene="hisB"
                     /locus_tag="VF362_03805"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX7590084.1"
                     /translation="MSRTARIARKTSESDVVVELDLDGTGVTDISTGVPFYDHMLTAL
                     GKHSLMDLTVKATGDVAIDSHHTVEDVAICIGEAIKQALGDKAGISRYGAAVVPLDEA
                     LAQAVVDVSGRPYFVHVGEPIGQATHLIGGNFAGSLTAHVFESIAHHAEITLHVRVLA
                     GRDPHHIVEAQFKALARALRAAVAVDPRVKGVPSTKGAL"
     gene            complement(2599..3693)
                     /locus_tag="VF362_03810"
     CDS             complement(2599..3693)
                     /locus_tag="VF362_03810"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX7590085.1"
                     /translation="MELPLRPELRGLSPYGAPQLDVPVLLNVNENPYAPPPSVVDAMA
                     AAVRKAAEGLNRYPDRDFVGLRTDLANYLAHEAHVDFLRHENIWAANGSNEVLLHLHQ
                     AFAGPGRVALSFDPTYSMYPEYSRDSFTTYVTLPRRDDFAIDVDAAVAEIGERRPTLV
                     LLASPNNPTGTALPLSDAVAIARAAADVPGGCVVVIDEAYAEFRRRGVPSAITLLPET
                     PNLAVTRTMSKAFVLAGGRLGYLAASPAIVDALRIVRLPYHLSAVTQAIARAALSHAR
                     ELQAQVDEIRAERDETVTWLREQGYAVADTDANFALFGTFGDRERVFRDLLERGVLIR
                     VVGPEGWLRVSIGTPQEMARFRAALMEVTA"
     gene            complement(3748..>4954)
                     /gene="hisD"
                     /locus_tag="VF362_03815"
     CDS             complement(3748..>4954)
                     /gene="hisD"
                     /locus_tag="VF362_03815"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX7590086.1"
                     /translation="RDFAQRFDGCRPEHLRVPLAAMGEALAGLDPVVREALEESIRRV
                     RIFHAATVPAPVSVEIAPGARVSQRWIPVRRVGLYVPGGLAVYPSSVVMNVVAAQEAG
                     VESIAVTSPPQSANGGLPHPTILAACALLGVDEVYAVGGAQAVAMLAYGATDGDATGE
                     GPDGSSSVLCEPVDVITGPGNRFVAAAKRAVQGVVGIDAEAGPTEIAVIADATADAEL
                     VASDLVSQAEHDPLAGSVLITDSEDFADAAEAATERRAGETKHSARVKEALTGAQSAI
                     VLTDGIEQSLAVANAYGAEHLEIMTADAAAVATRVTAAGCIFVGPYSPVSLGDYIAGS
                     NHVLPTGGTARFASGLGVTSFLRAVSVVEYDAAALGEVAARVVALANDEDLPAHGEAV
                     QARSIDGLG"
BASE COUNT          727 a         1768 c         1771 g          688 t
ORIGIN      
        1 ggtgggcgtc cgcgttgaag tcgtccgcgt cgaaaccacc cgcgccggac accagcccta
       61 cggccttccc gcgacgtcga gcgcctcggg aagcgtgaac gcgcccgcgt agagcgcctt
      121 cccgacgatg gcgccctcaa cgccgagcgg aacgagcgaa cgcagcgcca cgaggtcctc
      181 aagtgaggag atgcctcccg acgcgacgac gggcactcgg gtcgcggcgc acacctgagc
      241 gaggagttcc aggttgggcc cacgcagcgt cccgtccttg gtcacgtccg tgacgacgta
      301 gcgggagcag cccgcggcgt cgaggcgggc aagcacctcc cagaggtccc cgccctcctt
      361 ggtccagccg cgcgcggcga gggtcgtgcc gcgaacgtca aggccaacgg cgacgcgatc
      421 cccgtagcgg gcgatggcgc tcgcggtcca atccgggtcc tcgagcgcgg ccgtgccgag
      481 attgacccgg gcgcaaccgg tcgcgagggc ctgctcaagc gaggcgtcgt cgcggatgcc
      541 gccggacatc tccaccttga cgtcgagttg gcccaccact tcggcgagca tgaccgcgtt
      601 cgatccgcgc ccgaaggcgg cgtcgaggtc gacgaggtgg atccactccg ctcccccggc
      661 ctgccagtcg agggccgcgg tgatggggtc gccgtacgag gtctccgagc ccgcctcgcc
      721 ctggacgagt cggaccgcct gtccggccgc gacgtcgacg gcgggcagta gctggaggcg
      781 aggtgcgtcg ttcacagggt tccgatccag ttcttgagaa gcgcgagccc agcgggcccc
      841 gacttctccg ggtggaattg cgtggccgag agcggcccgt tctcgaccgc ggcgacgaag
      901 cgatcgtggt gtgcgggcgt gcccgcctcc gaccacgtga ccagtggctt cggcagtcgc
      961 ccctcgggct cgtcgttcag ggggaacgag cgcgcggcgt acgagtggac gaagtagaag
     1021 cgctcgttct ccacgccgca gaagagcaca gagccctcgg gcgcctccac cgtcgaccat
     1081 cccatgttcg ggacgacggg ggcgtccagc cggtcgacga tgccgggcca ctgggcgagg
     1141 ccgggagcgg tcgaaccgtg ctcctcgccc tcctcgaaca tcacctggag gccgacgcag
     1201 attccgagga cgggccggcc gcccgcgagg cgccgctcga cgatttggtc cccgcgcacg
     1261 gccctaaggc ccgccaggac cgccgcgaat gcacccacgc cgggcacgat caggccgtcc
     1321 gcgccctctg ccgcctggcg gtctgcggtg aggatcgcgg tcgcgccgac ctcctccacg
     1381 gcacgcacgg cgctccggac gttgccgaag ccgtagtcga gcacgcagac gaggggttgt
     1441 gccacgggcg aagtctatcg gggggcgccc acgcccctca gacccgcgcc cctcagccgc
     1501 gctgctcccg cttcgcggcg gccgcgaggc gccgcaggcg ccagatggcg cggaagcgcc
     1561 cagtgcgccc ccgatatacc ttgctcgggt cctccgcaag gagatcctcg atcgtcgcgc
     1621 ttcccagcat gagtcgctgg actccgccgg gcgcctgatc gagcgccagc cccggcggga
     1681 cgtcggcacc gcgctctccc cgctcccatc gggtgaccgt gacctggccg ccgcgccgct
     1741 ccatggcgag cagaacgatc tcgcggtgga gcgccgtcac ggccatggcc gcccgatccc
     1801 ccgcgccctc ggagtcgtcc gccaacaccg ccaaggtccc ctgcggcacc tcgatcacgt
     1861 cgcacttcgc gccgttgagg gcgcagatcg cggcgaggac cttcccgttc tggatggggg
     1921 tgaggagcgc gaccgtgtcg cgcgccaggt agccgggaag ctcgtcggtc acggcgtctc
     1981 cacaatcacg gcgtcgcctc aatcacagtg cacccttggt gcttgggacg cctttcactc
     2041 gcggatccac ggccacggcc gcgcgcaggg cgcgtgccag ggccttgaac tgcgcctcga
     2101 cgatgtggtg cgggtcgcgc cccgcgagca cccgcacgtg cagggtgatc tccgcgtggt
     2161 gcgcgatcga ctcgaaaacg tgcgccgtga gggagccggc gaagttgccg ccgatgaggt
     2221 gggtggcctg gccgatgggc tcgccaacgt ggacgaagta gggccggccc gagacgtcga
     2281 cgacggcctg cgcgagcgcc tcgtcgagcg ggacgacggc cgcgccgtac cgggaaatcc
     2341 ccgccttgtc gccgagggcc tgcttgatcg cctcgccgat gcagatcgcg acgtcctcga
     2401 cggtgtggtg cgagtcgatc gcgacgtcgc cggtggcctt gaccgtgagg tccatgaggg
     2461 agtgcttccc gagggcggtc agcatgtggt cgtagaacgg gacccccgtg ctgatgtcgg
     2521 taacccccgt gccgtcgaga tcgagctcga cgaccacgtc ggactcgctc gtcttgcgcg
     2581 caattcgggc cgtgcggctc atgcggtcac ctccattagc gcggcgcgga atcgcgccat
     2641 ctcctgcggc gtcccgatgg acacgcgcag ccaaccctcg ggcccgacga cccgaatgag
     2701 gaccccgcgc tcaagcagat cccggaatac cctctcacgg tccccgaagg tgccgaagag
     2761 ggcaaagttc gcgtcggtgt ccgcgaccgc gtagccctgc tctcgaagcc acgtcaccgt
     2821 ctcgtcccgc tcggcccgga tctcgtcaac ctgggcctgg agctctcgcg cgtgggagag
     2881 cgccgcgcgg gcgatcgcct gcgtcacggc gctgaggtgg tacgggagcc tcacgatcct
     2941 cagcgcgtcg acgatcgcgg ggctcgccgc gaggtagccg agcctgccgc ccgcgagcac
     3001 gaacgccttg gacatcgtgc gggtcaccgc gaggttgggg gtctcaggga gcagggtgat
     3061 cgcgctcggg accccgcggc gccggaactc ggcgtaggcc tcgtcgatga cgacgacgca
     3121 accgcccggg acgtcggccg ccgccctagc tatcgcgacc gcatcggaca gcggaagcgc
     3181 cgtccccgtc gggttgttcg ggctcgcaag caacaccagg gtggggcggc gctcgccgat
     3241 ctcggcgacg gccgcgtcca catcgatcgc gaagtcgtcc ctgcgcggga gcgtgacgta
     3301 cgtggtgaag ctatcgcgcg agtactcggg gtacatcgag tacgtggggt cgaagctcag
     3361 cgcgacgcgg ccgggcccgg cgaacgcctg gtgaaggtgc aggagcactt cgttcgagcc
     3421 gttcgccgcc cagatgttct cgtgccgaag gaagtccacg tgcgcctcgt gcgccaggta
     3481 gttcgccagg tccgtgcgga gccccacgaa gtcacggtcg gggtaccggt tcagcccctc
     3541 ggccgccttg cggaccgccg cggccatcgc gtcgacgacc gacggcgggg gcgcgtaggg
     3601 gttctcgttc acgttgagga ggacgggcac gtcgagttgg ggcgcgccgt agggcgacag
     3661 gccgcgcaac tcggggcgca ggggcaattc catggcgcaa gtctagtgag gggcggaggc
     3721 ggtgttctgg cgccgcgcag gtgcctccta gcccaagccg tcgatcgagc gagcctgaac
     3781 ggcctcgccg tgcgcgggga gatcctcatc gttcgccagg gcgacgacgc gcgcggcaac
     3841 ctcgcccagc gccgcggcat cgtactcgac gacggaaacg gcgcgaagga agctcgtcac
     3901 gcccaggccc gacgcgaagc gcgccgtgcc ccccgtggga agcacgtggt tggagccggc
     3961 gatgtagtcg ccgagcgaga cgggcgagta gggccccacg aagatgcagc cggctgcggt
     4021 gacgcgcgtt gccacggctg ccgcgtccgc ggtcatgatc tcaaggtgct ccgcgccgta
     4081 ggcgttcgcg accgcgagcg actgctcgat gccgtcggtg agcacgatcg cgctctgggc
     4141 gcccgtgagc gcctccttga cgcgcgcgga gtgcttcgtc tcgcccgcgc ggcgctccgt
     4201 ggcggcctct gccgcgtcgg cgaagtcctc ggagtccgtg atcagcaccg agcccgcgag
     4261 cggatcgtgc tcagcctggc tgaccaggtc agaggcgacg agttccgcgt ccgccgtcgc
     4321 gtccgcgatg acggcgatct cggtcgggcc agcctcggcg tcgatcccga cgacgccctg
     4381 caccgcgcgc ttggccgccg cgacgaacct gttgccagga ccggtgatga cgtcgacggg
     4441 ctcgcacaga acggaactgg agccgtccgg gccctcaccc gtcgcgtcgc cgtccgttgc
     4501 gccgtaggcg agcatcgcga ccgcctgcgc cccgccgacg gcgtagacct cgtcgacgcc
     4561 aaggagcgcg caggccgcga ggatcgtcgg gtgagggagc ccaccgttcg cggactgggg
     4621 agggctcgtc accgcgatgg attcgacgcc cgcctcctgg gccgcgacga cgttcatgac
     4681 gaccgagctc gggtacacgg ccaggccgcc gggcacgtag aggcccaccc tcctgacggg
     4741 gatccaccgc tgcgagaccc gggcccccgg cgcaatctcg acggagacgg gcgcgggaac
     4801 ggtggccgcg tggaagatcc tcacgcgccg gatggactcc tcaagcgcct cgcgcacgac
     4861 gggatcgagg cctgcgaggg cctcgcccat cgcggccagg ggaacccgca ggtgctcggg
     4921 ccgacagccg tcaaatcgct gcgcgaaatc gcgc
//
LOCUS       DASVKP010000187         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_224500, whole genome shotgun sequence.
ACCESSION   DASVKP010000187 DASVKP010000000
VERSION     DASVKP010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224500"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..>1592
                     /locus_tag="VF362_03820"
     CDS             92..>1592
                     /locus_tag="VF362_03820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7590087.1"
                     /translation="MEANVEFRVVGVGDEAALAEAFADIDETYFRPHPFTCDEARRIA
                     DYIGRDAYALLLERGRPVAYGMLRGWDEGYSVPSLGIAVRTTAQGRGLGRLMMAHLHA
                     EARGRGATVVRLRVNPDNVGARRLYESLGYAYAGEDRGELVMVADLESGTESRRDVGP
                     FPATLQGRLIDVDAPEWEVALRATRYDFYHLPAYVSLCAGQDRGRPCALYVTDGGHTL
                     LLPLVIRSVPGGGFDARSPYGYPGPVGVGVDDPAFLRVAFAAGLQVLRDADIVSAFVR
                     LHPLLNLVPPNGIGTLVRHGETVSIDLTHPIDDLWAQTRANHRRDITRALRLGYVAHM
                     DEDWQHFEAFKRLYRATMARRSAASFYFFEDSYFTGLRDALGESLHLCVVEKEGLVAA
                     AGLFVETRGIVQYHLSGTDDTFRTVQPTKLMLHFVRGWAKDRGNLIMNLGGGVGGDND
                     SLLQFKAGFSPSHHTFSTLRIVIDAREYGRLVEAHDPPLDPSACSGYFPL"
BASE COUNT          249 a          514 c          528 g          301 t
ORIGIN      
        1 gaatgaggtc ggtcctcggt cggcgtctcc ttcctctcgc agcctcggtc gactgacaaa
       61 ggatcgcccc tgagccgagc ccattcgaca ggtggaggca aacgtggagt tccgcgtcgt
      121 gggtgtgggc gacgaagcag ctctcgcgga ggcgttcgcg gacatcgacg agacgtactt
      181 ccggccacac ccgtttacgt gcgatgaggc tcgacgcatc gccgactaca tcggacgcga
      241 cgcctatgcg ctcctccttg agcggggtag acccgtcgca tacgggatgc ttcggggctg
      301 ggacgaaggg tattcagtcc cgtcgcttgg catcgcggtc cggacgaccg cccagggtcg
      361 ggggctgggg cggttgatga tggcgcatct ccatgccgag gcccgtgggc ggggagcgac
      421 cgtggttcgc ctccgggtga accccgacaa cgtcggggcg cggcggctgt acgaatcgct
      481 cggctacgcc tatgcgggcg aggaccgcgg ggaactcgtc atggtcgcgg atctcgaatc
      541 cggcaccgag tctcgaaggg atgtcggccc gttcccggcg actttgcagg ggaggctgat
      601 cgacgtcgac gcaccggagt gggaggtggc acttcgcgcg acgcgctatg acttctacca
      661 tttgccggcg tatgtctcac tgtgcgcggg gcaagacaga ggtcggccgt gcgccctcta
      721 tgtcacggat ggcggtcaca ccctgctgct gcccctcgtg atacgtagcg tccccggcgg
      781 cggattcgac gcgcggtctc catatggata ccccggcccg gtcggggtcg gggtcgacga
      841 tcctgcgttt cttcgggtcg ctttcgccgc cggtctccag gtgctgcgtg acgcggacat
      901 cgtttcggcg ttcgtccgcc ttcacccgct cctgaacctt gtgccgccga acggcatcgg
      961 cacgctcgtg cggcacggcg agaccgtgag catcgatttg acgcacccca tcgacgatct
     1021 ctgggctcag acccgtgcaa accatcgccg cgacatcacc cgcgcgcttc ggctcgggta
     1081 cgtcgcacac atggacgagg attggcagca cttcgaggcg ttcaagcgct tgtatcgggc
     1141 gacgatggcc cgtcggtcgg ccgcctcgtt ctacttcttc gaggactcgt acttcactgg
     1201 cctccgagat gccctcggcg agagcctcca tctttgcgtc gtggagaagg agggtcttgt
     1261 ggcggcggca ggcctgttcg tcgagacccg cgggatcgtc caataccacc tgtccgggac
     1321 ggacgacaca ttccggacgg ttcagccgac caagctcatg ctgcacttcg tgcgaggctg
     1381 ggcgaaagat cgcgggaacc tgatcatgaa cctcggaggc ggggtcgggg gagacaacga
     1441 ctcactgctg cagttcaagg ccggcttctc gccaagtcac catacgtttt caacccttcg
     1501 gatcgtcatc gacgcgagag agtacggccg cctcgtcgag gctcacgatc cgccgctcga
     1561 cccgagcgcc tgcagcggct atttccccct ct
//
LOCUS       DASVKP010000188         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_885044, whole genome shotgun sequence.
ACCESSION   DASVKP010000188 DASVKP010000000
VERSION     DASVKP010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885044"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VF362_03825"
     CDS             complement(<1..310)
                     /locus_tag="VF362_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590088.1"
                     /translation="MKTDLKTTDLKKTLPSYAAANGRCDIIDIPLLRYLAMDAKGAPE
                     DAEFGEAIGTLYPLAYALKFASKRDLGRDYVVPPLEALWWADDPASFTTARDRGKWRS
                     T"
     gene            369..1073
                     /locus_tag="VF362_03830"
     CDS             369..1073
                     /locus_tag="VF362_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX7590089.1"
                     /translation="MNRSERLFAIAEDLRGVGSRGRTTTQIAGLFGVSQRTIKRDMAA
                     LFASGIPLVSFEGRGGGYQVLPESHLAPVPLTAGEAAAVVIALASQPQMPYAAEGRSV
                     LAKMFAAMTPDQRAMATDAASRVWMRADPNVDRPPNATIIDDALRRQVAVTIVYTSSE
                     GKRTRRTIEPMAYVRTSGNWGVLAWCHLRGGGRWFRLDRITRAWPTNTPCTPRDLRAV
                     FGEPPADAMPAAALLA"
     gene            1222..>1586
                     /locus_tag="VF362_03835"
     CDS             1222..>1586
                     /locus_tag="VF362_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7590090.1"
                     /translation="MRRTLAALSAGLLVALLTACGTNPSEPTASPSPSASAAAPVAPM
                     TINIASLKGPTTMGLVKLMDDASKGTAAEDYQATVYGTPDEVIPLVVQGKLDIALIPA
                     NLAAVLYNKTKGTDAQVSVA"
BASE COUNT          246 a          594 c          512 g          234 t
ORIGIN      
        1 cggtcgagcg ccacttgccc cgatcgcgtg cggtggtgaa cgacgccggg tcgtccgccc
       61 accacagcgc ctccagcggc ggcaccacat agtcgcggcc gaggtcgcgc ttcgaggcga
      121 acttcagcgc ataggcgagc gggtacagcg tcccgatcgc ctcgccgaac tcggcgtcct
      181 ccggcgcgcc cttggcgtcc atcgcgaggt agcgcagcag ggggatgtcg atgatgtcgc
      241 accggccgtt cgcggcggcg tacgagggaa gggtcttctt caggtctgtc gtcttgaggt
      301 ccgtcttcat gccctcaccc tagggattgc gcttgacacc gtagtgtcac cgtttatacc
      361 gtcattccat gaaccgaagc gagcgcctct tcgccatcgc ggaggacctc cgcggagttg
      421 gcagccgcgg ccgaactacc acgcaaatcg ctggcctctt cggggtctcg cagcgcacca
      481 tcaagcgcga catggcggcg ctattcgcgt cgggcatccc ccttgtgtcg ttcgagggcc
      541 gcggcggcgg ctaccaggtg ctgcccgaat cgcatctggc cccggtgccc cttaccgcgg
      601 gcgaggcggc cgcggtggtg attgcgctgg cgtcgcagcc gcagatgccg tacgcggccg
      661 aggggcgctc cgtgctcgcg aagatgttcg cggcgatgac ccccgaccag cgcgccatgg
      721 cgaccgacgc cgccagtcgc gtgtggatgc gcgcggaccc caacgtcgac cgcccgccga
      781 acgccacgat catcgacgac gcgctccgac gccaggttgc cgtcaccatc gtctacacga
      841 gctcggaggg caagcggacg cgccgcacca tcgagcccat ggcctacgtg cgcacgagcg
      901 gcaactgggg cgtgctcgcg tggtgccacc tcaggggcgg cggccgatgg ttccggctgg
      961 accgcatcac gcgggcgtgg cccaccaaca ccccgtgcac cccgcgggac ctgcgtgccg
     1021 tcttcggaga gccgcccgcc gacgccatgc cggcggcggc ccttctggcc tagcgggccg
     1081 ggacgtcggg atgcccgcac ggcccgggac gtcagtcccc taggaccgag gtccgacgtc
     1141 gtcccggata ggcacgccta accttccaat aggtgccggt cgcagaagac ccgccgcaac
     1201 cccacgaaag gtcgatgtcc catgaggcga accctcgccg cactaagtgc cgggctcctc
     1261 gtcgcgctcc tcacggcgtg cggaacgaac ccgtccgaac cgacggcctc gccttcgcct
     1321 tcggcctcgg ccgcggcccc cgtcgccccg atgacgatca acatcgcctc gctcaagggc
     1381 ccgaccacga tgggcctcgt caagctcatg gacgacgcct ccaagggcac ggccgccgag
     1441 gactaccagg ccaccgtcta cggcacgccc gacgaggtca tcccgctcgt cgtccagggc
     1501 aagctcgaca tcgcactcat ccccgcgaac ctcgcggccg tgctctacaa caagacgaag
     1561 ggcaccgacg ctcaggtttc cgtcgc
//
LOCUS       DASVKP010000189         1952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_721528, whole genome shotgun sequence.
ACCESSION   DASVKP010000189 DASVKP010000000
VERSION     DASVKP010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721528"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /gene="ffh"
                     /locus_tag="VF362_03840"
     CDS             <1..1486
                     /gene="ffh"
                     /locus_tag="VF362_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX7590091.1"
                     /translation="EIRRALLDADVALPAVKAFTAAIRERALGAEVASALNPAQQVVS
                     IVHDELVEILGGASRPLARARTGPTIIMLAGLQGAGKTTLAGKLARHLKDLGETPVLV
                     AADLQRPNAVTQLTVVGQRAGIPVFAPERGVTREDEKPRGDPVKVAKASLKEAKDKVY
                     SVVIIDTAGRLGVDAEMMAQAASIRSAVSPHEVLYVVDSMMGQDAVATAHAFQEGVDV
                     TGVVLTKLDGDARGGAALSVRHVTGLPILFASTGEQVTDFEVFHPDRMASRILDMGDV
                     LTLIEQTQKAFDQKETDRIAAKVTKGEDFTLTDFLGQMQQLKNVGSMQKMLAMLPGMG
                     QAREAIENLDVKEVSRTEAIIYSMTPAERDDVGLLNGSRRARIAGGSGTTVTAVNSLV
                     KRFLEAQKMMKSMAKGGVPQIPGMAPGLAAATYGGAKRGKSAPKKSKGKSGNPAKRAQ
                     QEAEAERRASGRAGDAPAPAGSAFGLGAKAAPELPEVPGFLRRR"
     gene            1491..>1952
                     /locus_tag="VF362_03845"
     CDS             1491..>1952
                     /locus_tag="VF362_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX7590092.1"
                     /translation="MPPRAMTAAGEVLRLAGPILVDERTERGEAWVVGGRITFERPRF
                     SGDAQTIEGVVLPGLVDVHCHVGVTVGGAADDALAGKQAEADRDTGVLLIRDAGSATD
                     TSWVHDRADLPQLIRAGRFIARPKRYLRGFARELDRVEDLPLAAAEEARRGD"
BASE COUNT          304 a          680 c          715 g          253 t
ORIGIN      
        1 cgagattcgc cgcgcgttgc tcgatgcgga cgtcgccctt cccgcggtca aggccttcac
       61 cgccgcgatc cgcgagcggg ccctcggggc ggaggtcgcg agcgccctca accccgcgca
      121 gcaggtggtc tcgatcgttc acgatgagct ggtcgagatc ctcggtggcg cgtcgcggcc
      181 gctcgctcgc gccaggaccg gcccgacgat catcatgctc gcggggctcc agggcgcggg
      241 caagacgacc ctcgctggca agctcgcccg ccacctcaag gacctcggcg agacgcccgt
      301 cctcgtcgcg gccgacctgc agcggcccaa cgccgtcacc cagctgaccg tcgtcggcca
      361 gagggcgggc atccccgtgt tcgccccgga gaggggggtc acgagggagg acgagaagcc
      421 caggggcgac cccgtcaagg tcgcgaaggc ctccctcaag gaggcgaagg acaaggtcta
      481 ctccgtcgtc atcatcgaca ccgcgggccg cctcggcgtc gacgccgaga tgatggccca
      541 ggcggcgagc atccgctccg ccgtgtcccc gcacgaggtc ctgtacgtcg tcgactcgat
      601 gatgggccag gacgccgtcg cgaccgcgca cgcgttccag gagggcgtcg acgtgaccgg
      661 cgtcgtcctc accaagctcg acggcgatgc gcgcggcggc gccgccctct ccgtgaggca
      721 cgtgacggga ctgccgatcc tcttcgcctc gacgggcgag caggtgaccg acttcgaggt
      781 gttccacccg gaccggatgg cgtcccggat cctcgacatg ggcgacgtcc tcacgctcat
      841 cgagcagacc cagaaggcct tcgatcagaa ggagaccgac cggatcgccg cgaaggtcac
      901 caagggcgag gacttcacac tcacggactt cctcggccag atgcagcagc tgaagaacgt
      961 cggctcgatg cagaagatgc ttgcgatgct tcccggcatg ggccaggctc gcgaggcgat
     1021 cgagaacctc gacgtgaagg aggtctcgcg aaccgaggcg atcatctact cgatgacccc
     1081 ggccgagcgc gacgacgttg gcctcctcaa cggatccagg cgggcgcgca tcgcgggcgg
     1141 ctcggggacg acggtcacgg cggtcaactc gctcgtcaag cggttcctcg aggcgcagaa
     1201 gatgatgaag tcgatggcga agggtggcgt cccccagatt ccggggatgg cgccggggct
     1261 cgctgccgcg acgtacggcg gcgcgaagcg aggcaagagt gcgcccaaga agtccaaggg
     1321 gaagagcggc aaccccgcga agcgcgccca gcaggaggcg gaggccgagc gcagggcgtc
     1381 cgggcgggcg ggcgacgcgc ctgcgccggc gggcagcgcc ttcgggctcg gcgcgaaggc
     1441 cgcgccggag ttgcccgagg ttcccgggtt cctgcgcagg cgatgaccgc atgcctccgc
     1501 gggcgatgac cgccgcggga gaggtccttc ggctcgccgg ccccatcctc gtcgacgagc
     1561 ggacggagcg gggcgaggcc tgggtcgtcg gcgggcggat cacgttcgag cggccccggt
     1621 tctctggcga cgcccagacg atcgagggcg tcgttctgcc ggggctcgtc gacgttcact
     1681 gccacgtcgg agtgacggtg ggcggggccg cggacgacgc gcttgccggg aagcaggcgg
     1741 aggccgatcg cgacaccggg gtgctcctca tccgcgacgc cgggagcgcg actgacacct
     1801 cgtgggtgca tgaccgcgcc gatctgcccc agctgatccg cgccgggcgc ttcatcgccc
     1861 gaccgaagcg ctacctccgg ggcttcgcgc gcgagctgga ccgcgtcgag gatctcccgc
     1921 tcgcggcggc agaggaggcc cggcgcggcg ac
//
LOCUS       DASVKP010000190         4449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1049833, whole genome shotgun sequence.
ACCESSION   DASVKP010000190 DASVKP010000000
VERSION     DASVKP010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049833"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VF362_03850"
     CDS             <1..276
                     /locus_tag="VF362_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX7590093.1"
                     /translation="RNVLIVEDIVDSGRTLAWLVANLRSRGAASVEVAAMFRKPDAAV
                     IKTDVRYVGIEIPNEFVVGFGLDYAERYRGLPFVAALAPHVYGGAPS"
     gene            337..2220
                     /gene="ftsH"
                     /locus_tag="VF362_03855"
     CDS             337..2220
                     /gene="ftsH"
                     /locus_tag="VF362_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX7590094.1"
                     /translation="MNARRLLRSVVPWIIGGAALTWIVLGLSTPQVSRIDTSKGLQIL
                     ASGNVDQAKIVDGDQRVELTLKVADADYGKRVQFFYISQQGAAIIAAIEKADPPSGYN
                     AEVPQSSIWSTILFTFVPILLLVGLFWLLMSNMQGGSGKIMSFGRSRAKLVTADTPSV
                     TFADVAGVDEAVEELREIQEFLQTPEKFQAVGAKIPKGVLLYGAPGTGKTLLARAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKNSPAIIFVDEIDAVGRHRGAGLG
                     GGHDEREQTLNQMLVEMDGFDVRTNVILIAATNRPDILDPALLRPGRFDRQVPVDAPD
                     LKGRLRILEVHAKGKPISPDVNLEGIARRTPGFTGADLANVLNEAALLTARRDKKLLD
                     SSALDEAIDRVVAGPQKRTRVMNDHDKSVTAYHEAGHALVATAMLHTDPVTKVTILPR
                     GRALGYTMVMPIEDRYSKGRNQLLDTLAYSMGGRMAEELIFGDPSTGASSDIVQATEI
                     ARQMVTEFGMSAKVGAVHLSGGSGEVFLGRDIGHNRDYSEELAAVIDSEVRALMDGAA
                     REAADVLRTNKKVLDALASELLEKETLLQEDLVVIFKAVKKMPERKIWDSGHPKATRP
                     KRA"
     gene            2231..2815
                     /gene="folE"
                     /locus_tag="VF362_03860"
     CDS             2231..2815
                     /gene="folE"
                     /locus_tag="VF362_03860"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX7590095.1"
                     /translation="MDDGERGSVDRPRVEAAVREILIAIGEDPDREGLRETPGRVARS
                     YDELFAGLAQDPADTVSAVFDLGHHEMILVKDIEVYSICEHHLLPFHGVAHVAYIPGE
                     HGRITGLSKLARLVDVYARRPQVQERLATQIADALVEHVGARGVIVVLECEHLCMSMR
                     GVRKPGARTVTSAVRGVMRDAATRAEAMSLIVGR"
     gene            2824..3690
                     /gene="folP"
                     /locus_tag="VF362_03865"
     CDS             2824..3690
                     /gene="folP"
                     /locus_tag="VF362_03865"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX7590096.1"
                     /translation="MTEPRVTEPRVIEPRVIEPRCLVIGVLNVTPDSFSDGGRFEAVD
                     DAIAWGLQLRDDGADIVDVGGESTRPGAERVEPAVERQRIRPVVAALVAEGIRVSVDT
                     TRAETAAAAIEVGASLVNDVSGGLADPAMASVVAETGVDYIAMHWRAHSERMDELDAY
                     GDVVAEVRAELEARVAALTAAGIDPARIILDPGLGFSKVGQNNWPLLANLDSLVGLGH
                     RVVVGASRKRFLGGVITRDDGPLARDDATTAVTTLVADAGAWAVRVHDARAARDALGV
                     VSAWHGARRGGG"
     gene            3695..>4449
                     /gene="folB"
                     /locus_tag="VF362_03870"
     CDS             3695..>4449
                     /gene="folB"
                     /locus_tag="VF362_03870"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX7590097.1"
                     /translation="MTITVSPYFRNGVQLDTVVVEGIAAVGRHGVAASEREKGQPFVA
                     DVIAHLDTRLAGRDDELSKTVDYAKVATLAASHLAGDPVALIETLAERIAFAVLEIDT
                     VYAVDVVIHKPEAPIGVPVADTYVVIRRDVRGGDLWADKRIGSAAGLADDPQAPGAVP
                     EPKDELDERPGTAVPALVALGGNVGDVEFTLARAVEDLDRVAGIRVVAVSPLVSTKPV
                     GGPDQADFLNAVVRIETTLSPRSLLHVAQGIEMV"
BASE COUNT          709 a         1492 c         1542 g          706 t
ORIGIN      
        1 cgcaacgtcc tgatcgtgga ggacatcgtc gactccggcc gtacgctcgc gtggctcgtc
       61 gcgaaccttc gctcccgtgg ggcggcgtcg gtcgaggtcg cggcaatgtt ccgcaagccg
      121 gacgccgccg tcatcaagac ggacgtgcgc tacgtcggca tcgagatccc caacgagttc
      181 gtcgttggat tcgggctcga ctacgcggag cgctaccgcg gcctgccgtt cgtcgcggcc
      241 cttgccccgc acgtctacgg cggggcgccg tcctagtttc gccctgcgcg catccgtccg
      301 tcgctcggga cacgacaggt tagagtcgga ggcactatga acgcccgtcg tctccttcgc
      361 agcgttgtgc catggatcat cggcggcgcg gccctgacct ggattgtcct cgggctttcc
      421 acgccgcagg tgagccgcat cgacacctcc aaggggctcc agattctggc gtccggaaac
      481 gtcgaccagg ccaagatcgt cgacggagac cagagggtcg agttgacgct caaggtcgcg
      541 gacgccgact acggcaagcg cgtccagttc ttctacatct cccagcaagg cgcggcgatc
      601 atcgccgcca tcgagaaggc cgatcctccg agcggataca acgccgaggt cccgcagagc
      661 agcatctgga gcaccatcct cttcaccttc gtccccatcc tgcttctggt cggactgttc
      721 tggctgttga tgtcgaacat gcagggcggt agcgggaaga tcatgagctt cggacgctcg
      781 cgggcgaagc tcgtcaccgc ggacactccc tcggtgacct tcgccgacgt ggccggggtg
      841 gacgaggccg tcgaggagct ccgcgagatc caggagttcc tgcaaacgcc ggagaagttc
      901 caggccgtgg gagccaagat cccgaagggc gtgctcctct acggcgctcc cggaacggga
      961 aagaccctcc tggcgcgcgc cgttgcgggc gaggcgggag tgccgttttt ctcgatctcg
     1021 ggctcggact ttgtcgagat gttcgtgggg gtcggcgcga gccgcgtccg cgacctgttc
     1081 gagcaggcga agaagaactc gccggccatc atcttcgtgg acgaaatcga cgccgtcggg
     1141 cgccaccgcg gcgcggggct aggcggaggt cacgacgaga gggagcagac cctcaaccag
     1201 atgcttgtcg agatggacgg cttcgacgtc aggacgaacg tcattctgat cgcggcgacg
     1261 aaccggcccg atatcctcga ccccgcgctt ctgcggcccg gtcgcttcga ccgccaggtg
     1321 cccgtcgacg cccccgacct caaggggcgc ctacgcatcc tcgaggttca cgcgaagggc
     1381 aagcccattt cgcccgacgt caacctggag ggaatcgcgc gtcggacccc ggggttcacg
     1441 ggtgccgacc tcgccaacgt gctcaacgag gcggccctgc tcacggctcg tcgcgacaag
     1501 aagctcctcg actcgtcggc gctcgacgag gctatcgacc gcgtcgtcgc cgggccccag
     1561 aagcgcaccc gcgtcatgaa cgatcacgac aagagcgtga ccgcgtacca cgaggctggc
     1621 cacgcgctcg tggcgacggc gatgctccac acggatccgg tcaccaaggt gacgatcctg
     1681 ccgcgaggcc gcgcgctcgg ttacacgatg gtcatgccga tcgaggaccg ctactccaag
     1741 gggcgcaacc aactcctcga taccctcgcc tactccatgg gcgggcgcat ggccgaggag
     1801 ttgatcttcg gcgacccgtc cacgggggcc tcgagcgaca tcgtgcaggc caccgagatt
     1861 gcgcgccaga tggtgaccga gttcggcatg agcgcgaagg tcggggccgt gcacctctcg
     1921 ggcggctctg gcgaggtctt cctcggtcgg gacatcggcc acaaccgcga ctattcggag
     1981 gagctcgcgg cggtcatcga ctccgaggtt cgcgcgttga tggatggtgc ggcccgagag
     2041 gccgccgacg tcttgcgcac caacaagaag gtgcttgacg cactcgcgag cgaactgctt
     2101 gagaaggaga cgttgcttca ggaggacctc gtggtgatct tcaaggcggt caagaagatg
     2161 ccggaacgca agatctggga ttccggacac cccaaggcga ctcggcccaa gcgggcctag
     2221 agtggctctt gtggacgacg gagagcgcgg ttcggtggat cggccgcgcg ttgaggcggc
     2281 ggttcgggaa atcctgatcg ctataggcga ggaccccgat cgtgaaggcc tgagggagac
     2341 ccccggccgc gtcgcgcgct cgtacgacga actcttcgct ggcctcgcgc aggacccggc
     2401 cgataccgtc tccgccgtgt tcgacctcgg ccaccacgag atgatcctgg tcaaggacat
     2461 cgaggtctac tcgatatgcg agcatcacct gctgcccttc cacggcgtcg cccacgtcgc
     2521 gtacatcccc ggcgagcacg gacgcattac cggactgagc aagttggctc gcctggtgga
     2581 cgtctacgct cgccgcccgc aggttcagga gcggctcgcg acccagatcg cggacgcgct
     2641 cgtcgagcac gtcggtgccc gcggcgtgat cgtcgtgctc gagtgcgagc acctctgcat
     2701 gtccatgcgt ggcgtccgca agcccggggc gcgaacggtc acctccgccg tccgaggcgt
     2761 gatgcgagac gccgcgacca gggcagaggc gatgagcctc atcgtcggtc ggtaggcccg
     2821 ccggtgaccg aaccccgcgt gaccgaaccc cgcgtgatcg aaccccgcgt gatcgaaccc
     2881 cgctgcctgg tgatcggcgt cctcaacgtc acccccgact ccttctcgga cggagggcgg
     2941 ttcgaggcgg tggacgacgc gatcgcatgg ggcctccaac tgcgcgacga cggtgccgac
     3001 attgtcgacg tcggcggcga gtcgacccga ccgggcgccg agcgcgtcga gccggcggtg
     3061 gagcgccagc gaatccgtcc cgtggtcgcg gccctggtgg ccgagggcat ccgcgtgagc
     3121 gttgacacca ctcgcgccga gactgctgct gcggcgatcg aggtgggcgc gagcctcgtc
     3181 aacgacgttt ctggcgggct ggcggatccc gcgatggcga gcgtggtcgc cgagaccggg
     3241 gtggactaca tcgcgatgca ctggcgggcc cactccgagc gcatggacga gctcgacgcc
     3301 tatggcgacg ttgtcgccga ggtcagggcc gagcttgagg cgcgcgtcgc cgcgttgacg
     3361 gcggcgggca tcgaccccgc gcggatcatc ctcgatcccg gcctgggctt ctccaaggtt
     3421 gggcagaaca actggccgtt gctggcgaac ctggactccc ttgttggcct cgggcatcgc
     3481 gtcgtcgtgg gcgcctcgcg caagcgcttt ctcggcgggg tgatcacccg cgacgacggg
     3541 ccgctcgccc gcgatgacgc gacgacggcg gtcacgacgc tcgtggccga cgccggggcg
     3601 tgggccgttc gggtccacga cgccagggcg gcgcgtgacg cacttggcgt ggtgtcggct
     3661 tggcacggtg cccggcgcgg gggaggatga ggacatgacc atcaccgtct cgccctactt
     3721 caggaatggc gtccaactcg acaccgtcgt tgttgaggga atcgcggccg tggggcgcca
     3781 cggcgtcgcg gcctcggagc gggagaaggg ccagccgttc gtcgccgacg tcatcgcgca
     3841 cctcgacacg cgactcgcgg ggcgcgatga cgaactgagc aagaccgtgg actacgcgaa
     3901 ggtcgcgacg ctcgccgcca gccatctggc gggagatccg gtcgctctga tcgagacgct
     3961 cgcggagcgc atcgcgttcg cggtgctcga gatcgacacg gtctatgcgg tcgacgtcgt
     4021 catccacaag cccgaggcgc cgatcggggt gccggtggcg gacacgtacg tcgtgattcg
     4081 gcgcgacgtg cgcggcggcg acctgtgggc ggacaaacgg atcgggtcgg cggcgggcct
     4141 ggccgacgac ccgcaggcgc cgggggccgt tccggagccg aaggacgaac tcgatgaacg
     4201 ccccggcacg gccgttcccg ctctcgtcgc gctcgggggg aacgtcggag acgtcgagtt
     4261 cactctcgcg cgcgccgtgg aggacctcga ccgggtggcg ggcattcgcg tcgtcgccgt
     4321 ttccccgctg gtatcgacga agcccgtcgg cggtcccgac caggcggact tcctcaacgc
     4381 ggtcgtgagg atcgagacca cgctctcgcc gcgctccctc ctgcacgtcg cgcaaggcat
     4441 cgaaatggt
//
LOCUS       DASVKP010000191         4737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_225052, whole genome shotgun sequence.
ACCESSION   DASVKP010000191 DASVKP010000000
VERSION     DASVKP010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225052"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1341
                     /locus_tag="VF362_03875"
     CDS             172..1341
                     /locus_tag="VF362_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7590098.1"
                     /translation="MAARDTRSETLRRLRAGTGVLSTAAIKSLDSEYPWFRELPARER
                     SWVGAVAQAGISAFVAWYAAPTLPGRGAAEVFAAAPPELTRSISLQHTLSIVRTVVKV
                     VEDHCDDFASPGTEAELRMAILRYSREIAFSAAEVYARAAESRGAWDARLEALVVDAL
                     VRGQMDDDLPSRAAALGWKSGPTLVMVGLTDGPLGEVESAELRRTIRRASRGALVGIH
                     GEDLIVIARTDEDLAQLGAALLTSFGPGPVVIGPPAVTLIEVARSTRAALAGVIAAPA
                     WSLAPRPVAADDLLPERVLVGDASARERLLAIAYDPIARAGGALAETVETFLACGRSL
                     ELASRTMFVHANTVRYRLKKVTDLTGWDALSTRDAHVLDTAFALGRLRDSSHAPL"
     gene            1390..2370
                     /locus_tag="VF362_03880"
     CDS             1390..2370
                     /locus_tag="VF362_03880"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX7590099.1"
                     /translation="MAPKSGTLEDVLAVVCPGQGAQAPGMLSPWLEMPSVAERIARYA
                     DAAGLALVAHGTTSDADTIRDTAVAQPLLVATALATARELLGDAASEGNTALMDNTAP
                     SVAAGHSVGEFAAAALAGVFSDTDAMLMVSVRATAIATAAAASEPTSMAAVLGGDPDD
                     VLATLASFGLTPANVNGGGQVVAAGAREAIARLLAEPPAKSRVIELQVAGAFHTAYMK
                     PAVATLSAAAAGIAVADPTSILVSNADGSPVASGARALDLMVAQVANPVRWDLCQQTM
                     LDIGVTAMIEAAPGGVLTGLAKRSMPGIPCFALKSPADLAAARDLMETHS"
     gene            2376..3365
                     /locus_tag="VF362_03885"
     CDS             2376..3365
                     /locus_tag="VF362_03885"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX7590100.1"
                     /translation="MTINRGADYSRILGLGAARGENLVTNDDVAGPIDSSDEWIRQRT
                     GIITRVRANAETSVVDLAERASRDAIAASGVPVEEIDTVILSTFTYPTVTPAGAPILA
                     DRLGCGTAAAFDISAACAGYCYAIAQADALVRAGQSQHVLVIGAEKMSDWIDPTDRSI
                     SFLLGDGAGAIVIGPSTTPAIGPTVWGSKGDGAPLIGMTASWLDVRDRGAAFPRLFQE
                     GQPVFKWASFEMPKVAKHAIEAAGLTPEDIQAFIPHQANMRIIDQMIKTVGLRSDVAV
                     GRDIADTGNTSAASIPLATERLLREGTAKSGDLALQIGFGAGLVFAAQVVVLP"
     gene            3394..3642
                     /locus_tag="VF362_03890"
     CDS             3394..3642
                     /locus_tag="VF362_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010551652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX7590101.1"
                     /translation="MALSENEVLAGLAEIVAEETGVDAADVVGGKSFTDDLDIDSLSM
                     MTIVTLAEEKFDVRIPDEEVKNLVTVGDAVKYITGAQS"
     gene            3695..>4737
                     /locus_tag="VF362_03895"
     CDS             3695..>4737
                     /locus_tag="VF362_03895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX7590102.1"
                     /translation="MTKVAVTGLGMTTPLGGDVPSTWEAALDGRSGVRTLNNDWVERY
                     GLEVSFAGQVVVRPEEVLTRPEMKRMDPSAQMAIVATREAWADAGSPEVDPDRLGAAV
                     SSGIGGVWTLLNAWDTLKEKGAARVLPLTVPMLMPNSPAAYVELEVGARAGAHAPVSA
                     CASGAEAIATGYRMILDGRADVVVAGGTEAATHPVTLASFGAMQALSRRNDDPAGASR
                     PYDITRDGFVMGEGSGIIVLESEEFAKARGARIYAYLAGVGLTSDAYHIAAPEPHGRG
                     QVRAMQLALEAGGLSTQSVAHINAHATSTVVGDMIEARAIRTLLGTHADRTLLSATKS
                     MMGHLMGGAGAVES"
BASE COUNT          707 a         1764 c         1581 g          685 t
ORIGIN      
        1 aacgccgatc acggcgccga gcacggcgcc gagcaccggc ttgccacggg actcctgctg
       61 catggtggtg atctcctcga acgtgctctt aacctagcga ccggctcccg cgacgaccag
      121 caccgccacg ggtttgtcga gttcccacaa ggggccccta tgctgtcggg tatggccgcc
      181 cgcgacacac gctccgagac gcttcgccgg cttcgcgcag ggacgggcgt actgtcgacc
      241 gccgccatca agagcctcga ctccgagtat ccctggtttc gtgagttgcc cgcgcgcgag
      301 cgatcgtggg ttggcgccgt cgcccaagcg ggcatcagcg ccttcgtcgc ctggtacgcc
      361 gcgccgacgc tccccgggag gggcgccgcc gaggtcttcg ccgccgcgcc gcccgaactg
      421 acccgctcca tctccctcca gcacaccctg agcatcgtcc gcacggtcgt gaaggtcgtc
      481 gaggaccact gcgacgactt cgcctccccc ggcacggagg cggaactccg gatggcgatc
      541 cttcgctact cgcgggagat cgccttcagc gccgccgagg tctacgcgag ggccgcagaa
      601 tcgcgtggcg cgtgggacgc ccggctcgag gcgctcgtcg tcgacgcact cgtccgcggc
      661 caaatggacg acgaccttcc ctcccgagcc gcggcgctcg ggtggaaatc cgggccgacg
      721 ttggtgatgg tcgggctcac cgacgggccg ctcggcgagg tggagagcgc cgaactccgc
      781 cggacgatca ggcgggcctc gcgcggcgcc ctcgtgggga ttcacggcga ggacctcatc
      841 gtgatcgcgc ggaccgacga ggacctcgcg caactcggcg ccgcgctcct gacgagtttc
      901 ggccccggcc ccgtggtgat cggcccgccc gcggtgaccc tcatcgaggt cgcgcggtcc
      961 acccgcgccg ccctggccgg cgtcatcgcg gcccccgcat ggtccctcgc cccccgcccc
     1021 gtcgccgccg acgaccttct tcccgagcgg gtgctcgtcg gggacgcgtc cgcgagggag
     1081 cgcctcctcg cgatcgccta cgaccccatc gccagggcgg gcggggccct cgccgagacg
     1141 gtcgagacgt tcctcgcgtg cgggcgctcg ctcgaactcg cgtcgcgcac catgttcgtc
     1201 cacgccaata ccgtccgata ccgcctcaag aaggtcaccg acctcacggg ctgggatgcc
     1261 ctgagcaccc gcgacgcgca cgtgctcgac accgccttcg ccctcggtcg cctgagggat
     1321 tcgagccatg cgccgttgta ggaatcgcac aaaccacgcg ggcgacgttg gaggcctgcg
     1381 agtgggccct tggcgcccaa gtcgggcacc ctggaagacg tgctagccgt cgtctgcccg
     1441 ggacagggcg cccaagcccc ggggatgctc tccccgtggc tcgagatgcc ctccgtcgcc
     1501 gagcgcatcg cgcgctacgc cgatgccgcg gggctcgccc tcgtggccca cggcaccacc
     1561 tccgacgccg acacgatccg cgatacggcc gtcgcccagc ccctgctggt cgccaccgcg
     1621 ctcgcgaccg cccgcgagct tctcggcgac gctgcgtctg agggcaacac cgcgctgatg
     1681 gacaacaccg ccccgtccgt cgccgccggc cactccgtcg gcgagttcgc ggcggccgcg
     1741 ctcgccggcg tcttctctga taccgatgcc atgctcatgg tttccgtgag ggcgacggcg
     1801 atagcgaccg cggccgcggc gtccgaaccg acatcgatgg ccgccgtcct cggcggggac
     1861 cccgacgacg tcctcgcgac gctcgcgagc ttcggcctca cccccgccaa cgtcaacggc
     1921 gggggccagg tcgtcgccgc cggggcgcgg gaggcgatcg cccggctcct cgccgagccg
     1981 ccggccaagt cgcgcgtgat cgagctccag gtggcgggag ccttccacac ggcgtacatg
     2041 aagcccgccg ttgcgacgct cagcgccgcc gccgctggca tcgccgttgc ggacccgacg
     2101 tcgattctcg tgtccaacgc ggacggctcg cccgtcgcgt caggggcgcg cgccctcgac
     2161 ctgatggtgg cgcaggtcgc aaaccccgta cgctgggatc tgtgccagca gacgatgctg
     2221 gatatcggcg tgaccgcaat gatcgaagcc gcgccgggag gagtcctgac gggcctcgcc
     2281 aagcgcagca tgccgggcat cccatgcttt gccctcaaga gcccggcgga tctggccgcc
     2341 gcccgcgacc tcatggagac gcactcgtga cacagatgac gatcaaccgc ggggcggact
     2401 actcgcgcat cctcggcctc ggggccgcgc gcggcgagaa cctcgtcacc aacgacgacg
     2461 tcgcggggcc catcgactcg tccgacgagt ggatccgtca gcgcaccggc atcatcacga
     2521 gagtgagggc caacgccgag acctcggtgg ttgacctcgc ggagcgcgcc tcccgggacg
     2581 cgatcgccgc atccggagtc cccgtcgagg agatcgacac cgtcatcctc tcgacgttca
     2641 cgtacccgac cgtcaccccg gctggcgccc ccatcctcgc cgatcgcctt ggctgcggga
     2701 cggcggcggc cttcgacatc tccgccgcgt gcgccggcta ctgctacgcg atcgcccagg
     2761 cggacgcgct cgtgcgggcg ggccagagcc agcacgtcct cgtgatcggc gccgagaaga
     2821 tgagcgactg gatcgatcca accgaccgct cgatctcgtt cctgctcgga gacggcgcgg
     2881 gcgccatcgt cattggcccg tcgacgactc ccgccatcgg ccccaccgtg tgggggtcca
     2941 agggcgacgg cgcgccgctc atcggcatga cggcatcgtg gctcgacgtc cgcgaccgcg
     3001 gcgccgcatt ccccaggctc ttccaggagg gccagccggt cttcaagtgg gcgtcgttcg
     3061 agatgcctaa ggtggccaag cacgcaatcg aggcggccgg tctcacgccc gaggacatcc
     3121 aggccttcat cccccaccag gcgaacatgc gcatcatcga ccagatgatc aagaccgtgg
     3181 gccttcgcag cgacgtggcc gtcggccggg acatcgccga caccgggaac acgtccgcgg
     3241 cgtccatccc cctcgcgacc gagcggctcc tgcgcgaggg cacggcgaag tcgggagacc
     3301 tcgctctgca gatcggattt ggcgccgggc tcgtgttcgc cgcccaggtg gtcgttcttc
     3361 cctaaccgca tcacccaacc caaggagata cccatggcac tgtcagagaa cgaggtcctc
     3421 gcgggcctcg cagagatcgt cgcggaagag accggcgtcg acgccgccga cgtggtcggc
     3481 ggcaagtcct tcaccgatga cctcgacatc gactcgctgt cgatgatgac gatcgtcacc
     3541 ctcgcagagg agaagttcga cgtccgcatc cccgacgagg aggtcaagaa cctcgtcacc
     3601 gtcggcgacg ccgtcaagta catcaccggg gcgcagtcct aaccacggct cggcgcaaag
     3661 caccactcgt ccactcgcaa cgctcggagg agccatgacc aaggtcgccg ttaccggact
     3721 gggaatgacc acgcccctgg gcggagatgt tccgtcgacc tgggaggccg cgctcgacgg
     3781 ccgctcgggc gtccggacgc tgaacaacga ctgggtcgag aggtacggcc tcgaggtgtc
     3841 gtttgcgggt caggttgtcg tcaggccgga ggaggtcctg acgcggcccg agatgaagcg
     3901 catggacccc tcggcccaga tggcgatcgt cgcgacgcgt gaggcgtggg ccgacgcggg
     3961 ctccccggag gtcgatcccg atcgccttgg cgccgccgtc tcgtccggca tcggcggcgt
     4021 gtggacgctg ctcaacgcgt gggacaccct caaggagaag ggcgcggctc gcgtcctccc
     4081 cttgacggtt cccatgctca tgccgaactc ccccgccgcc tacgtggaac tggaggtcgg
     4141 ggcgagggcc ggcgcccacg cccccgtctc cgcgtgcgcg tccggagccg aggccatcgc
     4201 gacggggtac cggatgatcc tggacggccg cgcggacgtc gtcgtcgcgg gagggaccga
     4261 ggcggcgacc cacccggtca cccttgcgtc cttcggcgcg atgcaggcgc tctcgcgcag
     4321 gaacgacgat ccggcggggg cctcacgccc gtatgacatc acgcgggacg ggttcgtcat
     4381 gggcgagggc tcgggcatca tcgtcctcga atccgaggag ttcgccaagg ctcgcggggc
     4441 gcggatctac gcgtacctcg cgggcgtggg actgacctcc gacgcctacc acatcgccgc
     4501 ccccgagccc catggccggg gccaggtgag agccatgcaa cttgcgctcg aagccggggg
     4561 cctctccacc cagagcgtcg cccacatcaa tgcccacgcg acgtcgacgg tcgtgggcga
     4621 catgatcgag gcgcgcgcca tccggacgct tcttggcacg cacgctgacc gcacgctcct
     4681 cagcgctacc aagtccatga tgggtcacct aatgggtggc gcgggtgcgg tcgagtc
//
LOCUS       DASVKP010000192         3101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1217296, whole genome shotgun sequence.
ACCESSION   DASVKP010000192 DASVKP010000000
VERSION     DASVKP010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217296"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VF362_03900"
     CDS             <1..974
                     /locus_tag="VF362_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEX7590103.1"
                     /translation="RRSIRDMLELAVRVESFPTATQLQANVLEIRMIERYRPRYNRRS
                     SRPERTPWVRLTDEAYPRLSVVRSVSGDSTHIGPMRGAGDAKLAIEALHELGGVRTCT
                     TRLPVVPRRGARACLLADLGSCSAPCLHGAASGYGDVASALRDAIVGDPSPVVEALAA
                     RISGFAERLEFERASALRDGVSALVAGAAEAQRLAALRRVTIIAARPAGGAWEVAVVE
                     RGRLAGTACVQGGAAVAAAAARILGDDLSGADAGVAAAGGGAVPLIEEQQLIAAWLER
                     PGTRLVSVDGEWAQPVAGAARHAMWIDARREDRERVWDAYARSASGA"
     gene            complement(971..2302)
                     /locus_tag="VF362_03905"
     CDS             complement(971..2302)
                     /locus_tag="VF362_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEX7590104.1"
                     /translation="MTSRRLAARVAAGSALLVTAFLTAALVATLVAIGSPALATDTTP
                     TLAAESPPPIQDLTPIKDTVDFPVGVAIDARDMGGAPEELLLRHFSQVTPENDMKPEA
                     WYDASHKFAPRAEIAWLMDFASRNDLRVYGHVLVWYRQTPAWFFQHDDGTPLTASPED
                     QEILRNRMHDHIFAVAKYLSDNWGPFGGGNPVVAFDVVNEAVDAGAGDALGMRQSEWY
                     RVLGEEYVDLAFQYADEAFNTTYAAPGAERPVTLFINEVYTDQPATRAVYLALIDRLL
                     ARGAPIDGIGHQFHVNIGIPVTNLADALAESGGRGLVQAVTEMDVPTGTPTSDARFAM
                     QGDYYRDAFRIFREHSDEMFSVTLWGLYDARSWLDSRGGPLIFDDDLQAKPAYYGIVD
                     GQADQTPPNAADGGGGSPSPLPYVAGAAVLAALGAGGLVWSRRRRRRATRP"
     gene            complement(2323..>3101)
                     /gene="trpD"
                     /locus_tag="VF362_03910"
     CDS             complement(2323..>3101)
                     /gene="trpD"
                     /locus_tag="VF362_03910"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX7590105.1"
                     /translation="TVNISTMASIVAAGTGLTVVKHGNRAASSKSGAADVLAALGVNL
                     DLPPARVAELATEVGITFCFAQVFHPAMRFTAPIRKALGVPTTFNILGPLANPAQPAA
                     SAIGSGSPSLTPVMAGVLATQGRDALVFYSDDELDELAGTSPAQVWEVRGGTVTHTKL
                     DPVEDLGVTPITIADLRGGEASENAGVAWTVFSGKTGPVRDTVILNAAAGLVADGTLP
                     GTGTGSLPERFAAGMTLAAASIDDGKASDVLDRWVAASNA"
BASE COUNT          427 a         1071 c         1135 g          468 t
ORIGIN      
        1 agcgacgctc gatccgggac atgctcgaac tcgcggtgcg cgtggagtcg ttccctaccg
       61 cgacccagct ccaggcgaac gtcctcgaga tccggatgat cgagcgctat cggccccgct
      121 acaaccgtcg ctcgtcgcgc cccgagcgca ccccgtgggt gcgtctgacg gacgaggcct
      181 atccgcgcct cagcgtcgtg cggtccgtct ccggcgactc cacccacatc ggcccgatgc
      241 gcggagcggg agacgccaag ctcgcgatcg aggctctcca cgaactcgga ggcgtccgca
      301 cgtgtaccac tcgccttccc gtggtgccgc ggcgcggcgc gcgcgcgtgc ctcctcgccg
      361 acctcggctc gtgctcggcc ccgtgcctcc acggcgccgc gagcggttat ggcgacgtcg
      421 cctctgcgct tcgcgacgcg atcgtcgggg acccctcccc cgtcgtcgag gcgctcgccg
      481 cgcggatctc cgggttcgcc gagcgcctgg agttcgagcg ggcgtccgca ctacgcgacg
      541 gcgtgagcgc gctcgtcgct ggcgcggcgg aggcccagcg gctcgccgcg ctccgacgcg
      601 tgacgatcat cgcggcccgg cccgcgggcg gggcgtggga ggtcgccgtg gtggagcggg
      661 gacgcctcgc cggaacggcg tgcgtccagg gtggcgcggc ggtcgcggcg gccgcggcgc
      721 gcatcctcgg cgacgatctc tccggcgccg atgcaggagt agccgccgcg ggaggcggcg
      781 ccgttcccct catcgaggag cagcaactca tcgccgcgtg gctcgagcgg cccggaaccc
      841 ggctcgtgag cgtcgacggc gagtgggccc agcccgtcgc gggcgcggcg aggcacgcga
      901 tgtggatcga cgccaggcgt gaggatcgtg aacgcgtctg ggacgcctac gcccgctcgg
      961 cttcgggcgc ctagggccgc gtcgccctcc gtctgcggcg gcggctccac acgagcccgc
     1021 ccgcgccgag cgcggccaga accgcggccc ccgcgacgta aggcagcggc gagggcgatc
     1081 cgcctccccc gtccgctgcg ttgggcggcg tctggtcggc ctggccatcg acgatgccgt
     1141 agtaggcggg tttagcttgc aggtcgtcgt cgaagatgag cggtcccccg cggctatcga
     1201 gccagctcct ggcgtcgtac agcccccaca aggtgacgga aaacatctcg tcggagtgct
     1261 cgcggaagat gcggaacgcg tctcggtagt agtcgccctg catggcgaat ctggcgtcgg
     1321 acgtcggcgt cccggtcggc acgtccatct cggttaccgc ctgaacgagg cctcgcccgc
     1381 cggactcggc cagggcgtcc gcgaggttcg tgaccgggat tccgatgttc acgtggaact
     1441 gatggccgat gccgtcgatc ggtgcgccgc gcgccaacag gcggtcgatc agggcaaggt
     1501 agacggctcg cgtggcgggc tggtcggtgt agacctcgtt gatgaagagg gtcacgggac
     1561 gctcggctcc cggggcggcg tatgtggtgt tgaacgcctc gtcggcgtat tggaacgcga
     1621 gatcgacata ctcttcgccg agcacgcgat accactcgct ctgccgcatt cccagcgcgt
     1681 cgccagcgcc cgcgtccacc gcctcgttga ccacgtcgaa cgcgacaacg gggttccctc
     1741 cgccaaacgg gccccagttg tcgctgaggt actttgcgac ggcgaagatg tggtcgtgca
     1801 tccggttcct gaggatctcc tgatcctcgg gggaggcggt gaggggagtg ccgtcatcgt
     1861 gctggaagaa ccacgcgggg gtctgcctgt accagacgag cacgtggcca tagacgcgaa
     1921 ggtcgttccg ggaggcgaaa tccatcagcc aggcgatctc ggcgcgcggt gcgaacttgt
     1981 gggacgcgtc gtaccaggcc tcgggcttca tgtcgttctc tggcgtcacc tgcgagaagt
     2041 ggcgcagtaa cagctcctcc ggtgcgccac ccatgtcccg cgcgtcgatc gccactccga
     2101 cggggaaatc caccgtgtcc ttgatcgggg tcaggtcctg gatcggcggc ggactttcgg
     2161 cggcgagcgt cggcgtcgtg tcggtagcga gcgccggcga accgatcgcg accaacgtgg
     2221 cgacgagcgc cgcagtaagg aacgctgtga cgaggagcgc ggagcccgcc gcaaccctgg
     2281 cggcgagccg tcgcgacgtc acgcccatga atccccgcac ggctacgcgt tgctcgccgc
     2341 gacccagcgg tcgagcacgt ccgatgcctt gccgtcgtcg atcgacgcgg ccgcaagcgt
     2401 catgcccgcg gcgaagcgct cggggagcga gcccgtcccc gtcccgggga gcgtcccgtc
     2461 ggcgacgagg cccgcggccg cgttgaggat gacggtgtcg cgcacgggcc cggtcttgcc
     2521 ggagaagacc gtccacgcca ccccggcgtt ctcgctcgcc tcgcctccgc gaaggtcggc
     2581 gatcgtgatg ggcgtgaccc cgaggtcctc gacgggatcg agtttggtgt gcgtgacggt
     2641 cccgccgcgc acctcccaca cctgggcggg cgacgtcccg gcgagttcgt cgagctcgtc
     2701 gtcgctgtag aagacgaggg cgtcacggcc ctgggtcgcg agcacccccg ccatcacggg
     2761 cgtgagcgac gggcttccgg agccgatggc cgaggcggcg ggttgcgcgg ggttcgcgag
     2821 gggcccgagg atgttgaagg tcgtcggaac gccgagggcc ttgcgaatcg gcgccgtgaa
     2881 gcgcatcgcc gggtggaaga cctgggcgaa gcagaaggtg atgcccacct cggtggcgag
     2941 ttccgcgacg cgcgcggggg gaaggtcaag gttgacgccg agcgcggcca ggacgtccgc
     3001 ggcccccgac ttcgaactgg cggcgcggtt gccgtgcttg accaccgtca ggcccgtccc
     3061 cgcggcgacg atcgacgcca tggtggagat gttcacggtg t
//
LOCUS       DASVKP010000193         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_970506, whole genome shotgun sequence.
ACCESSION   DASVKP010000193 DASVKP010000000
VERSION     DASVKP010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970506"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..754
                     /locus_tag="VF362_03915"
     CDS             95..754
                     /locus_tag="VF362_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEX7590106.1"
                     /translation="MTVLGQLTATIADMATNRRVLVAFDGPDAGGKTTMANRVAAALP
                     GAVVRASVEGFHRRREVRMARGLDSPEGYYRDSFNLEALEDELLSPFLAGNELVRTRV
                     FDYRTDQAVDAEFEAVPPSATLIIDGVFLLRPELRRWWNLGIYVHVPDEVSLARAIVR
                     DSDVYGGPDETEVKYRSRYLPGQAIYRMEAHPQDAALIVIDNSDPDRPEVLKWDVAAR
                     V"
     gene            complement(815..>2150)
                     /locus_tag="VF362_03920"
     CDS             complement(815..>2150)
                     /locus_tag="VF362_03920"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEX7590107.1"
                     /translation="ADLTYNPTTLDALETTSPGFPWRAWADALGVPEGGRDKLVAQQP
                     SFFADLADLWAGGEAGLDLDAWKAWLRWRIVSGRYPYLTRELSRANFDFYGTVLSGAP
                     EQRHRWKRGVGLVDGVLGEAVGREYVARHFPSTHKARMEELVANLVEAYRQSIAALPW
                     MTSETKERALEKLGQFTPKIGYPDRWRDYSALALDPTDLMANVRAATAFEEDYDWGRL
                     LKPTDRSEWFTFPQTVNAFYNPTGNEIVFPAAILQPPFFDAEADDAVNYGAIGSVIGH
                     EIGHGFDDQGSKFDGLGRLDDWWTEADRVAFSERTKALIQQFDAYCPAQLGPEHHVNG
                     ALTIGENIGDLAGVEIGLKAYAISLGGSLDDAPVLDGFTATQRYFLGYAVTEQTLRRD
                     EALITQINSEPHAPEEFRINGVVRNMDAWYEAFGVGPDDALWLPPEERVSIW"
BASE COUNT          308 a          725 c          753 g          364 t
ORIGIN      
        1 gccaatgcga cgtttcgagc gccgtcatgt cgcattcctc acaagaaagc cggatttcct
       61 gtattaccgg ggggcgcatg gctatgtttc gagggtgaca gttctcggac aactgactgc
      121 gacgatcgct gacatggcga cgaaccgccg cgtcctcgtc gcgttcgacg ggccggacgc
      181 cggggggaag acgacgatgg cgaatcgagt cgccgccgcg cttccagggg ccgtggtgcg
      241 cgcgtcggtc gaaggcttcc accgtcgccg cgaggttcgc atggctcgcg gcctggactc
      301 ccccgagggc tactaccggg attccttcaa cctcgaggcg ctcgaggatg agttgctgtc
      361 tcccttcctg gcgggcaatg aactcgtgcg cacgcgcgtc ttcgactacc gcaccgacca
      421 ggcggtcgat gccgagttcg aggccgttcc cccctcggcg accctcatca tcgacggggt
      481 cttcctgctc cgccccgaac ttcgccggtg gtggaacctc gggatctacg tgcacgtgcc
      541 ggacgaggtc tcgctcgccc gcgcgatcgt ccgcgactcc gacgtgtacg gcggccccga
      601 cgagacagag gtgaagtacc ggtctcggta ccttcccggg caggcgatct accgcatgga
      661 ggcgcacccg caggacgcgg ccctgatcgt catcgacaac tcggatccgg accgcccaga
      721 ggtcctgaag tgggatgtcg cggcccgcgt ctgacctgag ggccacgcct gccgatctcg
      781 ctcgcccgtc tcgcccgtcg gccccccggc cgcgctacca gattgagacg cgctcctcgg
      841 gtggtagcca gagggcatcg tcggggccca cgccgaacgc ctcgtaccac gcgtccatgt
      901 tgcgcaccac gccgttgatc cggaactcct ccggcgcgtg cggctccgag ttgatctggg
      961 tgatgagcgc ctcgtcgcga cgcagtgtct gctccgtgac ggcgtagccg aggaagtagc
     1021 gctgggtcgc ggtgaagccg tcgagcacgg gcgcgtcatc gaggctcccc ccgagcgaga
     1081 ttgcataggc cttgaggccg atctcgacgc ccgcgaggtc gccgatgttc tcgccgatgg
     1141 tcagcgcgcc gttgacgtgg tgctccgggc ccagctgggc ggggcagtat gcgtcgaact
     1201 gctggatcag cgccttggtg cgctcggaga atgccaccct gtcggcctcg gtccaccagt
     1261 cgtcgagccg cccaagccca tcgaatttgg agccctggtc gtcgaacccg tgcccgatct
     1321 cgtggccgat gacgctccct atggcgccgt agttgaccgc gtcgtccgcc tcggcgtcga
     1381 agaagggggg ttgcaggatg gcggcgggga agacgatctc gttgcccgtg gggttgtaga
     1441 acgcgttgac ggtctgcggg aaggtgaacc actcgctgcg gtccgtcggc ttgaggaggc
     1501 ggccccagtc gtagtcctcc tcgaacgccg tggcggcgcg gacgttggcc atgaggtccg
     1561 tggggtcgag ggcgagggcc gagtagtcgc gccagcggtc ggggtagccg atcttcggcg
     1621 tgaactggcc gagcttctcc agcgcgcgct ccttggtctc gctcgtcatc cagggaaggg
     1681 cggcgatgga ctggcggtac gcctcgacga ggttcgcgac cagttcctcc atgcgcgcct
     1741 tgtgggtgga agggaagtgc cgcgcgacgt actcgcggcc cacggcctcg ccgagtaccc
     1801 cgtccacgag cccgacgccc cgcttccatc ggtggcgctg ctctggggcc ccgctcagga
     1861 cggtgccgta gaagtcgaag ttggcgcgcg agagctcgcg ggtcagatac ggataccgtc
     1921 ccgacacgat gcgccaccgc agccacgcct tccaggcgtc gaggtcgagg cccgcctcgc
     1981 cccccgccca gaggtcggcg aggtccgcga agaacgaggg ctgctgcgcc acgagtttgt
     2041 cgcgcccgcc ctcggggacg ccgagcgcgt cggcccacgc gcgccacggg aagccggggg
     2101 acgtggtctc gagggcgtcg agcgtcgtcg ggttgtaggt gaggtccgcc
//
LOCUS       DASVKP010000194         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_805298, whole genome shotgun sequence.
ACCESSION   DASVKP010000194 DASVKP010000000
VERSION     DASVKP010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805298"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VF362_03925"
     CDS             <1..961
                     /locus_tag="VF362_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX7590108.1"
                     /translation="MRRFGSDKPDLRFGLELIELTDYFKDTPFRVFQAPYVGAVVMAG
                     GASQPRKTLDAWQEWAKQRGAKGLAYVLVEQSGELGGPVAKNLSEEERAGLAAAVGAS
                     PGDCVFFAAGPVESSRALLGAARIEIAKRLDLIAPATFEFTWVVDAPLFKPVNSDDDD
                     VAVGSGAWTAVHHAFTSPKPEFMDTFDKAPGEALAYAYDIVCNGNEIGGGSIRIHRRD
                     VQERVFDVMGIGAVEAQEKFGFLLDAFAFGAPPHGGIAFGWDRIVTILAGTDSIRDVI
                     AFPKSGGGYDPLTGAPAPIPAQQRKETGVDYKPEQKKVAAPEA"
     gene            complement(986..>2169)
                     /locus_tag="VF362_03930"
     CDS             complement(986..>2169)
                     /locus_tag="VF362_03930"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX7590109.1"
                     /translation="VAMTLVPALAYVFGHRLERPGLVSRIKGLRRVLRITADVTKDEG
                     LFSRLTARVQRRPWLVVAGSLAILAILASPALGIQLRNSGLELLPKGDADRVFVTDFD
                     ANFPQLRDPDVRLVAEASASELQAWEAANLASLGGVMGVTPTTESGDLAFSGLILSFP
                     DRGGADAVNLVKEIRNLNPPFRLYVGGAAANQVDFIGAITSGAPLALGIVILGTFVLL
                     FFMTGSLLVPLKALLISTLSLSASIGVAVWIFQHGNLEGPLQFSSVGGLETYVVVLIL
                     AFGFGLSMDYEVFLLSRIKELVDKGVPNDEAVRLGLQRSGRIITSAATIVIVVFLGFT
                     AGKILVIKELGVGLAFAVLLDATLVRLLLVPATMTLLGKWNWWAPKPLKRLHAGMGIR
                     H"
BASE COUNT          376 a          718 c          763 g          312 t
ORIGIN      
        1 gatgcgtcgc ttcggctcgg acaagcccga tctgcgcttc ggtctggaac tcatcgagct
       61 cacggactac ttcaaggaca cgcccttccg cgtcttccag gcgccgtatg tgggggccgt
      121 cgtgatggcg ggtggcgcat cccagccccg caagacgctc gacgcgtggc aggagtgggc
      181 gaagcagcgc ggcgccaagg gactcgccta cgtcctggtc gagcagtccg gcgaactcgg
      241 cggcccggtc gccaagaacc tttccgagga agagagggcc ggacttgccg ccgccgtcgg
      301 cgcctctccg ggcgactgcg tcttcttcgc cgccggtccc gtcgagtcgt cgcgcgccct
      361 gctcggcgcg gcgcgcatcg agatcgccaa gcgcctcgac ctgatcgcgc ccgcaacctt
      421 cgagttcacg tgggtggtcg acgccccgct cttcaagccc gtcaactcgg acgatgacga
      481 cgtggccgtc gggtccggcg cctggacggc cgtgcaccac gccttcacca gccccaagcc
      541 cgagttcatg gacaccttcg acaaggcgcc gggggaggcc ctggcgtacg cctacgacat
      601 cgtctgcaac ggcaacgaga tcggcggcgg ctccatccgt atccaccgcc gcgacgttca
      661 ggagcgagtc ttcgacgtca tgggcatcgg cgcggtagag gcgcaggaga agttcggctt
      721 cctgctcgac gccttcgcct tcggcgctcc gccccacggc ggcatcgcgt tcgggtggga
      781 ccgcatcgtc acgatccttg ccggcacgga ctcgatccgc gacgtcatcg ccttccccaa
      841 gtcgggaggg gggtacgacc cgctgaccgg ggctcccgcg ccgattcccg cgcagcaacg
      901 caaggagacg ggcgtcgact acaagccgga gcagaagaag gtcgcggccc cggaggcgta
      961 ggccgggccg acactcgccg atgccctagt gcctgatccc catccccgca tggagccgct
     1021 tgaggggctt gggcgcccac cagttccact tgccgagaag cgtcatcgtc gccgggacga
     1081 gcaggaggcg gacgagcgtc gcgtcgagga ggacggcaaa cgcgagcccg accccgagtt
     1141 ccttgatgac gaggatcttg cccgccgtga agcccaggaa gacgacgatg acgatcgtcg
     1201 cggccgaagt gatgatgcgt cccgagcgct ggagcccaag ccggaccgcc tcgtcgttgg
     1261 gcacgccctt gtccacgagt tccttgatgc gtgacagcag gaagacctcg tagtccatcg
     1321 agagcccgaa cccgaaggcg aggatcagga cgacgacgta cgtctcgagt ccgcccaccg
     1381 aactgaactg gagcgggcct tcgaggttgc cgtgctggaa gatccagacg gcgaccccga
     1441 tggatgcgct gagcgacagg gtgctgatca ggagagcctt gaggggcacg aggagggagc
     1501 ccgtcatgaa gaacagcagg acgaaggtgc cgaggatgac gatccccagc gcgagcggcg
     1561 cgcccgaggt gatcgcaccg atgaagtcca cctggttggc ggccgcgccg cccacataga
     1621 ggcggaaggg cgggttgagg ttgcggatct ccttcacgag gttgaccgcg tcggcaccgc
     1681 cccgatcggg gaacgagagg atgaggccgg agaaggcgag gtcgcccgac tcggttgtcg
     1741 gcgtcacgcc catcacgccg ccgaggctcg cgaggttcgc ggcctcccag gcctgaagtt
     1801 cggaggcgga cgcctccgcg acaagccgga cgtcggggtc gcgcaactgc gggaagttcg
     1861 cgtcgaagtc cgtcacgaag acccggtcgg cgtcgccctt ggggaggagt tcgagtcctg
     1921 agttgcgcag ttgaatgccc agggcggggc tggcgaggat ggcgaggatg gcaagggagc
     1981 ccgccacgac gagccagggc cgcctctgga cccttgccgt gaggcgggag aagagaccct
     2041 cgtccttcgt cacgtcggcc gtgatgcgga ggacccgtcg gaggcccttg atccgcgaga
     2101 cgagcccggg acgctcgagt cggtggccga acacatacgc gagggccgga accagcgtca
     2161 tggcgacca
//
LOCUS       DASVKP010000195         3446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_556217, whole genome shotgun sequence.
ACCESSION   DASVKP010000195 DASVKP010000000
VERSION     DASVKP010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556217"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VF362_03935"
     CDS             <1..897
                     /locus_tag="VF362_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX7590110.1"
                     /translation="WWDSGVTSFEPSLYMSELADGGVVSREGWEPRPETNPSVEAPQL
                     SAPWPPDATRAQRRIRDLAAEVGEVVSRGASGDVEALPHGREIAAMLAEQAVRSGRAS
                     RVSMPTHLSATELVWLARDADAFARDLRRPIPKEPTEAAARGSAFHGWVEAHYGVVSL
                     WDDEDAEGSGGDIGALRDAFLASAWAARLPLATEARVEVPLGSLTLRSQIDAVFPAGA
                     GLDRVTVVDWKTGAPPKDPGERAAREVQLATYRLAWSRWTGIPIEEVDAVFFYASTGQ
                     TVAPERMLGEEEIVALVLAAGR"
     gene            complement(928..1854)
                     /locus_tag="VF362_03940"
     CDS             complement(928..1854)
                     /locus_tag="VF362_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7590111.1"
                     /translation="MPHSPLALAALASAAVPGLDVYDVLRSPHADVDFDVVIVVDSTG
                     KHWMVRAPRRAAAGAALEAELGLLSHLAREYDSGSLSFAVPRPAGTAALPEGGRAVVY
                     EEIRGTRLNLAALEPGPGLAASLGHAIAEIHELRPEIIEDQGLPTYTPEAYRQRRLAE
                     LDAAAQTGKVPPALAGRWEERLENVAWWRYEPTPVHGSLGEHQVVVADGAVAGIVGWA
                     EARVADPADDLAWLIASATPEAADSVIEAYSVGRRELRDPHLVDRARLAAELALARWL
                     MHGIRTEEPEIVDDAQDMLADLEAQLTEAGEL"
     gene            1924..2835
                     /gene="nudC"
                     /locus_tag="VF362_03945"
     CDS             1924..2835
                     /gene="nudC"
                     /locus_tag="VF362_03945"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEX7590112.1"
                     /translation="MHERWPLAEAPLIDRAAHLRTSVDLDACDAVVVVGGTVLVDNGR
                     LAILPAAVRPPARFAFYLGRIASGDVAGRDVAGIVPEGELALLPSAAMVPLRGAFADL
                     DDEAARGDRELATTAVAMATWHERAAHCPLCGTATEPREGGWSRRCPTDGVDHYPRTD
                     PAVIVAITDPDDRMLLAHVSYHSPLRYSHLAGYVEPGESLEQAARREVFEESGVTLAE
                     LDYAGSQPWPFPASIMIGFRARAASTDIVVDGVEVTDAAWLTRPELAQRVAAGDMILA
                     PPGSIARRLIHTWYGGPVPDADGVSSA"
     gene            2849..>3446
                     /locus_tag="VF362_03950"
     CDS             2849..>3446
                     /locus_tag="VF362_03950"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX7590113.1"
                     /translation="MRPDDILEPLDPEQREVARALGGPVCVLAGAGTGKTRAITHRIA
                     YGVSVNAYQAPSVLAVTFTARAAGEMRQRLRALGVDGVQARTFHAAALRQLGYFWPEV
                     VGGQIPPIAPQKGALVGEAARRVGMSVDRIAVRDLASEIEWAKVSLVAAGDYAAVAEA
                     QGRPQPAGVARALVADLMREYEDVKTEKVLLDFEDILLI"
BASE COUNT          466 a         1201 c         1289 g          490 t
ORIGIN      
        1 tggtgggact cgggggtgac ttctttcgaa ccgtcgctgt acatgagcga actcgcggat
       61 ggtggtgtcg tctcccgcga ggggtgggag ccccgcccgg agaccaaccc ctccgtcgag
      121 gcgccccaac tgagcgctcc gtggccgccg gacgccacgc gcgcccaacg gaggattcgg
      181 gacctcgctg ccgaggtggg ggaagtcgtg tcgcgtgggg cgtcggggga cgtcgaggcg
      241 cttccgcacg ggcgcgagat cgcggcgatg ctcgccgagc aggcggttcg gtccggtcgc
      301 gcctcgcgcg tctccatgcc cacccacctc tcggcgaccg aactggtgtg gctcgcgcgc
      361 gacgcggacg cgttcgcgcg tgaccttcgt cggcccatac ccaaggagcc gacggaagcg
      421 gcggcaaggg ggagcgcctt ccacggctgg gtcgaggccc actacggcgt ggtgtcgcta
      481 tgggacgacg aagacgcaga agggtcaggg ggcgatatcg gcgcgttgcg cgacgccttc
      541 ctcgcctccg catgggcggc gcggctgccc ctggccacgg aggcccgcgt cgaggttccc
      601 ctaggctctc tgacgctgcg gtcgcagatc gacgcggtct tccccgcggg cgcggggctc
      661 gaccgggtca cggtcgtcga ctggaagacc ggggcgccgc ccaaggatcc tggggagcgc
      721 gccgctcgcg aggtccaact cgcgacctat cgcctcgcgt ggtcgcggtg gacgggcatt
      781 cccatcgagg aggtcgacgc cgtcttcttc tacgcctcga cgggtcagac ggtcgcgccc
      841 gaacgcatgc tcggcgagga ggagatcgtc gcgctcgtgc tcgccgcagg ccgctagagt
      901 gcgcaggccg ctagggtccg caggccgcta gagttcgccg gcctccgtga gctgggcctc
      961 gaggtcggcg agcatgtcct gggcgtcgtc gacgatttcg ggctcctccg tgcggatgcc
     1021 gtgcatgagc cagcgcgcga gggcgagttc ggccgcgaga cgcgcgcgat ccaccaggtg
     1081 gggatcccgc agttccctgc gacccacgga gtacgcctcg atcacggagt cggcggcctc
     1141 cggcgtcgcg gaggcgatga gccatgccag gtcatccgcg gggtcggcga cgcgcgcctc
     1201 ggcccagccg acaatcccgg cgaccgctcc gtcggcgacg acgacctgat gctcgccaag
     1261 gctcccgtgc acgggggtgg gctcgtaccg ccaccacgcg acgttctcga gccgctcttc
     1321 ccatcgcccg gcgagggcgg gcggcacctt gccggtctgc gccgcggcat cgagttccgc
     1381 gaggcggcgc tgacggtagg cctccggcgt gtacgtcggt aggccctggt cctcgatgat
     1441 ctcggggcga agttcatgaa tctcggcgat cgcgtggccg agcgaagccg cgagccccgg
     1501 tcccggctcg agcgcggcga ggttgaggcg ggttccgcga atctcctcgt agaccacggc
     1561 gcgccccccc tcgggaaggg cggcggtccc ggcggggcgc ggcaccgcga acgagagcga
     1621 acccgagtcg tactctcgcg ccaggtgtga caggagtccg agctcggcct cgagcgcggc
     1681 gcccgcggcg gcccgacgcg gcgcgcgcac catccagtgc tttcccgtcg agtcgacgac
     1741 gatgaccacg tcgaagtcga cgtcggcgtg cggactccgg aggacgtcgt acacgtcgag
     1801 tccggggacc gcggccgacg cgagagcggc gagggcgagt ggagagtggg gcacgacctc
     1861 acggtagagc caaagggggg gtgggcgcca cgcgccacca cgcgcggcgg ctaccgtgag
     1921 gggatgcatg aacgctggcc cctcgccgaa gcgccgttga tcgatcgcgc cgcgcacctc
     1981 cgcacttccg tggatctcga cgcgtgcgac gccgtcgtgg tcgtcggcgg gacggtgctg
     2041 gttgacaacg gccgcctcgc gatccttccc gccgcggtgc ggcccccggc ccgattcgcg
     2101 ttctaccttg ggcgcatcgc cagcggggac gtcgcgggca gggatgtggc gggcatcgtt
     2161 cccgagggcg agcttgcact cctgccatcg gcggcgatgg tgcctctgcg gggcgcattt
     2221 gccgacctcg acgacgaggc ggcgcgtggg gaccgcgaac tcgccacgac cgcggtcgcg
     2281 atggcgacct ggcacgagcg cgcggcccac tgccccctgt gcggaactgc gaccgagccg
     2341 cgcgagggag ggtggtcgcg ccgctgcccc accgacggcg tggaccacta cccgaggacg
     2401 gatcccgccg tcatcgtggc gatcacggat ccggacgatc ggatgctcct cgcccacgtc
     2461 tcctaccact cgcccctgcg gtactcgcac ctggcggggt acgtcgagcc gggggagtcg
     2521 ctcgagcagg cggcgcgacg cgaggtgttc gaggagtcgg gcgtcacgct cgccgaactc
     2581 gactacgccg ggagccagcc gtggcccttc cccgcgtcga tcatgatcgg gttccgcgcg
     2641 cgcgccgcct cgacggacat cgtggtcgac ggcgtggagg ttaccgacgc cgcgtggctc
     2701 acgcggccgg aactggcgca gcgggtcgcg gcgggcgaca tgattctggc gcccccagga
     2761 tcgatcgctc ggcgcttgat ccacacctgg tacggcggtc cggtcccgga cgccgacggt
     2821 gtctcctccg cgtgacacga tcgccctcat gagacccgac gacattctcg agccgctcga
     2881 tcccgagcag cgcgaggtcg cgcgggcgct gggcggcccc gtctgcgtgc ttgcgggcgc
     2941 ggggacgggc aagacgcgcg caatcaccca ccgcatcgcc tacggcgtga gtgtcaacgc
     3001 ctaccaggct ccctcggtgc tcgcggtgac gttcacggca cgcgcggccg gagagatgcg
     3061 ccagaggctg cgcgcgctgg gcgtcgacgg ggtgcaggcg cgcacattcc acgcggccgc
     3121 cctgcggcaa ctcgggtact tctggcccga ggtggtaggg gggcagatcc ccccgatcgc
     3181 cccgcagaag ggcgccctgg tgggcgaggc ggctcgacgc gtggggatga gcgtcgaccg
     3241 gatcgcggtg cgcgaccttg cctccgagat cgaatgggcc aaggtctcgc tggtggcggc
     3301 gggtgactac gcggccgtcg ccgaggcgca ggggcggccc cagcccgcag gggtcgcgag
     3361 agccctggtc gcggacctca tgcgcgagta cgaggacgtc aagaccgaga aggtcctcct
     3421 cgacttcgag gacatcctcc tgatcc
//
LOCUS       DASVKP010000196         1582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_972323, whole genome shotgun sequence.
ACCESSION   DASVKP010000196 DASVKP010000000
VERSION     DASVKP010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972323"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VF362_03955"
     CDS             complement(<1..1167)
                     /locus_tag="VF362_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7590114.1"
                     /translation="MTETAARYPLHRKGEVVASPHFPSIERDVLAYWKSDGTFQASID
                     NRPATSAADEFVFYDGPPFANGLPHYGHLLTGYAKDVVPRYQTMRGKRVERRFGWDTH
                     GLPAELEAERILGITDKAQIEEMGIAAFNQACRESVLKYTKEWEEYVTRQARWVDFEN
                     DYKTLDLGYMESVIWGFKQLYDKGLAYEGYRVLPYCWKDETPLSNHELRMDDDVYQSR
                     QDPALTVALRLDDASVAASTTLTPEQKAALTGASLLIWTTTPWTLPSNLAVAVGPEIE
                     YDLVRPRSGGFAGRKLVIASRRRASYARELLPDVGDEIETLAALVGSDLVGLRYEPLF
                     PYFADHSDAFRVLAADFVTTEDGTGIVHLAPAFGEDDMIACEVAGIEPVIPIDSQ"
BASE COUNT          235 a          517 c          546 g          284 t
ORIGIN      
        1 ctgggaatcg atgggaatca cgggctcgat gcccgcaacc tcgcaggcga tcatgtcgtc
       61 ctcgccgaac gccggggcga ggtggacgat cccggttccg tcctcggtcg tgacgaagtc
      121 cgcggcgagg acgcggaagg cgtcggagtg gtcggcgaaa tacgggaaga ggggttcgta
      181 gcggaggccg acgaggtcgg agccgacgag cgccgccaac gtctcgatct cgtcccctac
      241 gtcggggagc agttcgcgcg cgtagctcgc gcgacggcgc gacgcaatga cgagcttccg
      301 tccggcgaat cccccgctgc ggggccgcac caggtcgtat tcgatctccg gaccgacggc
      361 gacggcgagg ttggacggaa gcgtccaggg cgtcgtcgtc cagatgagca gcgacgcccc
      421 ggtgagcgcc gccttctgct cgggcgtcag cgtcgtcgac gcggcgacgg aagcgtcgtc
      481 caaccggagc gcaaccgtca gcgcggggtc ctggcgcgac tggtagacgt cgtcgtccat
      541 gcggagttcg tggttggaaa gaggcgtctc gtccttccag cagtacggga gcacgcggta
      601 gccctcgtag gcgaggccct tgtcgtacag ctgcttgaag ccccagatga ccgactccat
      661 gtatccgagg tcgagggtct tatagtcgtt ctcgaagtcc acccatcgcg cctggcgggt
      721 gacgtactcc tcccactcct tggtgtactt gagcacgctc tcgcggcacg cctggttgaa
      781 ggccgcaatc cccatctcct cgatctgcgc cttgtccgtg atgcccaaga ttcgctcggc
      841 ctcgagctcg gccgggagcc cgtgggtgtc ccacccaaac cgccgttcca cccgcttgcc
      901 ccgcatggtc tggtaccggg gcacgacgtc cttggcgtag cccgtcaaga ggtgcccgta
      961 gtgcgggagg ccattggcga aggggggccc gtcgtagaag acgaactcat cggccgcaga
     1021 tgtggccggt cggttgtcga tcgacgcctg gaacgtgcca tccgacttcc agtaggcgag
     1081 tacgtctcgc tcgatcgagg ggaagtgggg ggaggcgacg acctcgccct tgcggtggag
     1141 gggatagcgg gcggcggtct cggtcatgtc gttgcggtcc tcggcgtcgg gtggtcctta
     1201 cgtactgttg cgaggacgcc tgcagaagcc gacgcggtac caccccgctt gccgccagct
     1261 ctcgccggtg accactcgtc ccctcccttg cgggagggcg ccgctataac gggctgcccg
     1321 tccggctcta ctgacccccg aggccggggg cgttcttccg gaggctcgcc ggtgatgacc
     1381 gggtcgacgc ctgtggcgcc agtgtagcgc gcgggtcacg tagcgatttc gttgttaaat
     1441 gacacccctt atgcccccga gtcgctaggt accctggggg gtatgacgaa gcgtcgtctc
     1501 cccgacgccg gggtgtcggt cggcctggtt gccctactcc tcaccctcgc cgcgtattcg
     1561 gcggatccca tcccaacctc gg
//
LOCUS       DASVKP010000197         3614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1464774, whole genome shotgun sequence.
ACCESSION   DASVKP010000197 DASVKP010000000
VERSION     DASVKP010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464774"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..2938)
                     /locus_tag="VF362_03960"
     CDS             complement(179..2938)
                     /locus_tag="VF362_03960"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020014879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX7590115.1"
                     /translation="MATLSQGFDPSLHAADEVVAEAQADAAVGLSTQEARVRLERDGA
                     NQLVARRPIPAWRRLLSHFADPLVYLLLAATVVALTAWWVEGREGVPYDVIVIAVVIL
                     ANAALGFIQERRSEDAVAALQRMAAPTAAVLRDGRIDRIPALDVVVGDVLVLGAGDAI
                     TADARLTESTLSVAEAALTGESAPVDKGVAPLDGPAGLGDRACMVFAGTAVTRGAGRA
                     IVTATGMHTETGRIAQLLGETASDPTPLQREIAQLSRVLGLGVLAIAAVVIGTVFLTE
                     EVTTLAGAVQVLLLGVALAVAAVPEGLPAILSVVLALGVQRMAKRHAIIKDLSSVETL
                     GSASVICSDKTGTLTQNRMTITRVLTVSGETELTGAAGGLPEGARGAEVTAVMTGGAL
                     ANDALVSGDEDDPTFAGDPTEVAFLTAEWELGLAVDRRARFSQLGSVPFSSERKRMSV
                     VASDADGGFYVAAKGAPGVLLEACTAELAGEQERPLDEAGHARVLAGVARISAMGMRT
                     LAVAFRREPKESAAEAESLERDLVLAGIVGIVDPPRDEARDAVAAAQGAGIRVIMITG
                     DHPLTASAIAVALGIVEADPEVVTGVQLAEMDGDALREAVKTTSVFARVAPEHKLRIV
                     DALQSHGEVVAMTGDGVNDAPALRSADIGVAMGLDGTEVARGASNMILTNDDFATIVL
                     AVNEGRGILDNIKRFLRYMFASNLGEVLAVFLGVVGAAWLGLRDASGAVVAPLLASQI
                     LWMNLLTDLGPALAIGAEPYADDLMSRGPRRPSERLIDRRMWGGIALTGAVMAVVTLF
                     AIDLYLPGGLVNGSGDVATARTAAFTVLVLTQLMNAFSARSETQSIVHQLLTNPWLWG
                     AVTLSLALQIAVVNVPVLNDAFSTAPLTIDQWSQCLGLALVVPVVMEARKWLLRARDR
                     RALAR"
     gene            3100..>3614
                     /locus_tag="VF362_03965"
     CDS             3100..>3614
                     /locus_tag="VF362_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7590116.1"
                     /translation="MRWATQDITREAPGTLPGLARVANLIRSVQTPGFEGVTFHEVAA
                     KSALNRVPGASAMPYDWTINPYRGCSHACTYCFARPSHTYLGLDAGRDFDSQVVVKVN
                     VAEVLRSELARPSWRREWVALGTNTDPYQRAEGRYRLMPGIISALADSGTPFSILTKG
                     TVLSRDLDLLAA"
BASE COUNT          521 a         1322 c         1266 g          505 t
ORIGIN      
        1 ggcgacgtct tcgcgtggcc cgcgccgctt ccgctcgcca acaccttcag cgtcggcgac
       61 gtcctcatcg tgatcggtgt cttcgtcgcc gcgtggacgg ggacgcgccg cctcggggcc
      121 gccgaggccc cgccgacgga cgaggagaag gcgtagattg ttccgccccg gcggtgagtc
      181 agcgcgccag ggcccggcgg tcccgcgccc ggagcagcca cttgcgcgcc tccatcacca
      241 cgggcacgac gagcgccagc ccgaggcact ggctccactg atcgatcgtc agcggcgcgg
      301 tcgagaacgc gtcgttgagc accggcacgt tcaccaccgc gatctgaagg gcgagggaga
      361 gggtcaccgc cccccacagc catgggttcg tcagcagttg gtggacgatg ctctgggtct
      421 cggaccgcgc cgagaaggcg ttcatcagtt gggtgaggac gaggacggtg aaggccgccg
      481 tgcgggccgt cgccacgtcc cccgagccgt tcacgagccc gcccggcagg tatagatcga
      541 tcgcgaagag cgtcacgacg gccatgacgg cccccgtgag cgcgatgccg ccccacatgc
      601 gccggtcgat cagccgctcg gacggccgcc gcggcccgcg tgacatcagg tcgtcggcgt
      661 agggctcggc gccgatcgcc agcgcgggcc ccaagtccgt gagcaggttc atccagagga
      721 tctggctggc cagtagcggc gcgaccacgg ccccgctcgc gtccctcagt cccagccacg
      781 ccgcgccgac cacccccagg aataccgcga gcacctcgcc gaggttcgac gcgaacatgt
      841 agcgcaggaa gcgcttgatg ttgtcgagga ttccgcggcc ctcgttcacc gccaagacga
      901 tcgtcgcgaa gtcgtcgttg gtcaggatca tgttcgacgc cccgcgcgcc acctccgttc
      961 cgtcaaggcc catcgcgacg ccgatgtcgg cgctacgcag ggcgggggcg tcgttgacgc
     1021 cgtcgccggt catcgcgacc acctcgccgt ggctctggag cgcgtcgacg atgcgcaact
     1081 tgtgctcggg cgccacgcgg gcgaacaccg aggtcgtctt gacggcctcg cgcagcgcgt
     1141 cgccgtccat ctcggcgagt tggacgccgg tgacgacctc ggggtccgcc tcgacgatgc
     1201 cgagggcgac ggcgatggcc gacgcggtga gcggatggtc gccggtgatc atgatgacgc
     1261 ggatgcccgc gccctgcgcc gccgcgaccg cgtcccgcgc ctcgtcgcgc ggcgggtcga
     1321 cgatgccgac gatgccagcg agcaccaggt cgcgctcgag cgactcggcc tccgccgcgg
     1381 actccttcgg ctcccggcgg aacgcgacgg cgagcgtgcg catccccatg gccgatatgc
     1441 gcgcgacccc ggcgagtacg cgcgcgtggc cagcctcgtc gagcggccgc tcctgttctc
     1501 ccgcaagctc cgccgtgcag gcctcgagca ggacgcccgg cgcccccttg gcggcgacgt
     1561 agaagccgcc gtccgcgtcc gacgcgacca ccgacatgcg cttgcgttcc gagctgaaag
     1621 gcacggaccc gagctgggag aaccgggcgc gcctgtccac cgccagcccg agttcccact
     1681 ccgccgtgag gaacgctacc tcggtggggt cgcccgcgaa cgtcgggtca tcctcgtccc
     1741 cgctcacgag ggcatcgttc gccagcgcgc cgcccgtcat caccgcggtg acctcggcgc
     1801 cgcgcgcgcc ctcggggagc ccgcccgcgg cgcccgtcaa ttccgtctcg cccgacaccg
     1861 tgaggacgcg cgtgatcgtc atgcggttct gggtgagggt tcccgtcttg tccgagcaga
     1921 tgacggacgc ggagccgagc gtctcgacgg aggagaggtc cttgatgatc gcgtggcgct
     1981 tggccatccg ctgcaccccg agggcgagca ccacggacag gatcgcgggg agcccctctg
     2041 gcaccgcggc caccgcgagg gcgacgccaa gcaggagcac ctggaccgcg ccagcgagcg
     2101 tggtgacttc ctcggtgagg aagaccgtcc cgatgacgac ggcggcgatc gcgagcaccc
     2161 cgagcccgag caccctgctc aactgagcga tctcgcgctg caggggcgtg gggtccgacg
     2221 ccgtctcgcc cagcagctgg gcgatgcggc cggtctcggt gtgcatgccg gtggccgtga
     2281 cgatggcgcg tccggcgccg cgcgtcactg ccgtgcccgc gaagaccatg cacgcccgat
     2341 cgccgagccc tgcgggcccg tcgagcggcg ccacgccctt gtcgaccggg gcgctctcgc
     2401 ccgtcagcgc cgcctccgcg acggagagcg tggattcggt gagccgggcg tcggccgtga
     2461 tggcgtcgcc cgccccgagc accaggacgt cgccgacgac gacgtcgagg gcggggatgc
     2521 ggtcgatgcg gccgtcgcgt agaacggcgg cggtcggggc ggccatccgt tggagcgcgg
     2581 ccaccgcgtc ctcggagcga cgctcctgga tgaatccaag ggcggcgttg gcgaggatga
     2641 cgaccgcgat cacgatcacg tcgtagggga ctccctcgcg gccctcgacc caccacgcgg
     2701 ttagcgctac cacggtggcc gcgagcagga gatacaccag cggatccgcg aagtggctga
     2761 ggagtcgtcg ccacgcgggg atggggcgcc gcgctaccag ttggttcgcc ccgtcccgct
     2821 caaggcggac gcgggcctcc tgggtgctga ggccgacggc agcatcggcc tgcgcctcgg
     2881 cgactacctc gtccgcggcg tggagcgagg ggtcgaaccc ctgcgacaac gtggccatat
     2941 gacactcccg tcgccgttct tcgtttgtgg gtcgctctca ccccattgtg gccctcagac
     3001 aacggtgccc cgcggccccg gcgagggcca tcccgatgcg cgcggagaac cacggcgagc
     3061 ccgcttgacg gcgcctcgaa cacctgttcg aatgggggca tgcgctgggc gacgcaggac
     3121 atcacgcggg aggcgcccgg cacgttgcct ggcctcgcgc gcgtggcgaa cctgatccgc
     3181 tcggtccaga cgcccggttt cgagggcgtc accttccacg aggtcgcggc caagagcgcg
     3241 ctcaaccgcg ttccgggcgc gagcgccatg ccctacgact ggaccatcaa cccctaccgg
     3301 ggatgtagcc acgcgtgcac gtattgcttc gcgcgcccga gccacaccta cctcggcctc
     3361 gatgccggcc gggacttcga ctcccaggtg gtcgtcaagg tcaacgtcgc cgaggtcctg
     3421 cggagcgaac tcgcgcgccc gtcatggagg cgtgaatggg tcgcgctcgg gaccaacacc
     3481 gacccttacc agcgcgccga ggggcgctac cggctgatgc cgggcatcat ctcggcgctc
     3541 gcggacagcg ggacaccgtt ctcgatcctg accaaaggca cggtgctgtc ccgcgatctc
     3601 gatctgctgg ccgc
//
LOCUS       DASVKP010000198         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1304396, whole genome shotgun sequence.
ACCESSION   DASVKP010000198 DASVKP010000000
VERSION     DASVKP010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304396"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /gene="pta"
                     /locus_tag="VF362_03970"
     CDS             <1..1173
                     /gene="pta"
                     /locus_tag="VF362_03970"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015770935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEX7590117.1"
                     /translation="ATDLGTFEAATICSSTRGRLTRDSGLKIDTARALFEKHVDCDAL
                     RRIFNVASTDVVTPLMFEYALLDRARKNKRHIVVPEGDDDRILQAASTLLRRGVVDLT
                     ILGNEATIRARAAELGVDISNAAIISPSDEQYVARFAAKFAELRASKGVTVDKAREIV
                     QDVSYFGTMMVLEGLADGMVSGAKHTTAHTITPAFQVIKTPPGVGIVSSVFLMCLSDR
                     VLVYGDCAVNPDPTAEQLADIAISSAATAVQFYIEPRIAMLSYSTGASGTGTDVEKVR
                     AATEIVKARRPDLPIDGPIQYDAAIEPSVASAKAPDSVVAGRATVFVFPDLNTGNNTY
                     KAVQRSAGAVAIGPVLQGLRKPVNDLSRGATVQDIVNTVAITAIQAQQIDATEGAA"
     gene            1170..>1604
                     /locus_tag="VF362_03975"
     CDS             1170..>1604
                     /locus_tag="VF362_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEX7590118.1"
                     /translation="MTAPRGGVALVLNCGSSSVKYQVVDTMQAEPLASGLIERVTDHT
                     EAIEAIIESLAAPGSGVSLDDLIVVGHRVVQGGSEFSAPTIITDDVERHITLLSTLAP
                     LHNPPNLAGIAAARVAFPLLPHVAVFDTAFHHTLPEAAYTYAI"
BASE COUNT          284 a          585 c          484 g          251 t
ORIGIN      
        1 gcgacggatc tgggaacctt cgaggctgcg accatctgct cgagcacccg cgggcgcctt
       61 acgcgcgact cagggctcaa gatcgacacc gcccgcgccc tgttcgagaa gcatgtggac
      121 tgcgatgcgt tgcgccggat cttcaacgtt gcgtcgacgg acgttgtcac acccctgatg
      181 ttcgagtacg cgcttctcga tcgcgcccgc aagaacaagc gccacatcgt cgttcccgag
      241 ggcgacgacg accggattct ccaggccgcg tcgaccctgc tcagacgcgg agtggtggat
      301 ctcaccatcc tcggaaacga ggccaccatc cgcgctcggg cggccgaact cggcgtcgat
      361 atttcgaacg ccgcgatcat cagcccctct gacgagcaat acgtcgcccg cttcgccgcg
      421 aagttcgccg agctgcgcgc cagcaagggc gtcaccgtgg acaaggcgcg ggagatcgtc
      481 caggacgtga gctacttcgg cacgatgatg gtgctcgagg gcctcgcgga cgggatggtc
      541 tcgggtgcga agcacaccac ggcgcacacg atcacgcccg cattccaggt gatcaagacg
      601 ccgcccggag tgggaatcgt gtcctcggtc ttcctcatgt gcctatccga tcgcgttctc
      661 gtctacggcg actgcgccgt caaccccgat cccacggcgg agcaactcgc cgacatcgcc
      721 atctcgtccg ccgcgactgc ggtccagttc tacatcgagc cccggattgc gatgctctcg
      781 tactcgacgg gagcctccgg gacgggaacg gacgtcgaga aggtccgcgc ggcgaccgag
      841 atcgtcaagg cccgccgccc cgacctcccc attgacggtc cgatccagta cgacgcagca
      901 atcgagccga gcgtcgcgag cgcgaaggcc cccgactccg tcgtcgccgg acgcgcgacg
      961 gtcttcgtgt tccccgacct caacacgggc aacaacacct acaaggccgt tcaacgctcg
     1021 gcgggagccg tcgccatcgg gccggttctc cagggccttc gcaagcccgt caacgacctc
     1081 tcaaggggtg caaccgtgca ggacatcgtc aataccgtcg ccattacggc gattcaggcc
     1141 cagcaaatcg atgcgaccga gggggcggca tgacggcccc cagggggggc gtcgccctcg
     1201 tcctcaactg cggctcaagc tcggtgaagt accaggtcgt cgacacgatg caggccgagc
     1261 cgctggcgtc cggcctcatc gagcgcgtga ccgaccatac cgaggcgatc gaggcgatca
     1321 tcgagtcgct tgccgctccg ggaagcggtg tcagcctcga cgacctcatc gtcgtcggac
     1381 accgcgtggt ccagggaggg agcgagttca gcgcccccac catcatcacc gacgacgtcg
     1441 agcgccacat caccctcctg agcacgctcg cccccctcca caaccctccg aacctcgcgg
     1501 gaattgcggc ggcgcgcgtc gccttccccc tgctcccgca cgtcgcggtc ttcgacacgg
     1561 ccttccacca cacgctgccc gaggctgcgt acacctacgc gatc
//
LOCUS       DASVKP010000199         2906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_558403, whole genome shotgun sequence.
ACCESSION   DASVKP010000199 DASVKP010000000
VERSION     DASVKP010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558403"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VF362_03980"
     CDS             complement(<1..245)
                     /locus_tag="VF362_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010079405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7590119.1"
                     /translation="MDALVEGLNPQQAEAVLYRGPALLIVAGAGSGKTRVLAHRIAHL
                     LASGRARPHQILAITFTNKAAAEMRDRVAALVGPESRG"
     gene            324..983
                     /locus_tag="VF362_03985"
     CDS             324..983
                     /locus_tag="VF362_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590120.1"
                     /translation="MAATTDPKVLEEHRWPAVVALLAALTLYALLPSSFLPGLRYAVI
                     AAGLVALVPLIVINPVRFQRETGWSRRLSLGQILLLVVANQVALVQLIDQLINANVTN
                     ARVLLLSALQVWFTNVIVFALMYWEIDRGGPVARSGGGSGRTHRPSLRFPQEETPPVP
                     NWRPEFLDYLFTSVWTSTAFSPTEAMPLAHRVKLLMAIESVAGLVVFALAVGRSMNLL
                     S"
     gene            complement(1104..1760)
                     /locus_tag="VF362_03990"
     CDS             complement(1104..1760)
                     /locus_tag="VF362_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7590121.1"
                     /translation="MSDRIRVVVVDDHDVIRVGVKQSLGEGVEVVGEGRDVESAIQAI
                     ADARPEVVILDVHLPGGMGGGALDVLDACLKKPEPPRFLALSVSDAAEDVVAVVRAGA
                     RGYVTKSISGPDLTDAVKRVALGDAVFSPRLAGFVLDAFGAAAGDVAMVDEDLDKLTA
                     REQEVMRLIARGYSYREVGEKLFISIKTVETHVSAVLRKLQLSSRHELAHWAAQRRIV
                     "
     gene            complement(1757..2749)
                     /locus_tag="VF362_03995"
     CDS             complement(1757..2749)
                     /locus_tag="VF362_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7590122.1"
                     /translation="MPNAAWPRALVGAAFLMAAFARASALFGWPRPAAWVAPLVAYVV
                     GLALVWSPLDLASVEDARRPEVVVGLFARDHWARVLVGIGLAFCAMWWFASWEFTDQP
                     VVRLVMVPPVLVVALALLFGPWLVRLARQVRVERSERIREFERAEIAAHLHDSVLQTL
                     TLIRKNSDDPATVAKLARAQERDLRTYLYQDRRTAAESVATALAAAISSVEDAHAVAI
                     DVVSVGDAPTDKRLRAAVAAAKEAASNAAKHGVAPISVYAEVSDDRYQVFVRDSGPGF
                     NPKRIPADRLGIRESIVGRVERHGGFAAVASRRGQSTEVTISVPRSAENGKVAR"
BASE COUNT          449 a         1029 c          971 g          457 t
ORIGIN      
        1 ccccgcgatt cgggaccgac gagcgccgcc acgcgatccc gcatctcggc ggccgccttg
       61 ttggtgaaag tgatcgcgag gatctggtgc ggccgcgcgc ggccggaggc gagcaggtgg
      121 gcgatgcgat gggccagcac ccgggtcttg ccggagcccg cccccgcgac gatgaggagc
      181 gcggggccgc gatagaggac ggcctcggcc tgttggggat tgagaccctc gaccagagca
      241 tccatgacgc gtcgagtcta ggcgttggat gcgacccctc gcggcgaggg cgggcggccc
      301 cacgactcgc ctagactggg gcgatggcgg caactactga tcccaaggtt ctcgaggagc
      361 accggtggcc agccgtcgtt gccctgctcg cggccctcac gctgtacgcc ctgctaccga
      421 gttcgttcct cccgggcctc cggtatgccg tgatcgccgc ggggctcgtg gcgctcgttc
      481 cgctcatcgt gatcaatccc gtgcgtttcc agcgggaaac ggggtggtcg cggcgtttat
      541 ctcttggcca gatcctgctc cttgtggttg ccaaccaggt tgccctcgtc cagttgatcg
      601 accaactcat caacgccaac gtgaccaacg cgcgggtcct gctcctgtcc gcgctgcagg
      661 tgtggttcac gaacgtcatc gtctttgcgc tcatgtactg ggagatcgat cggggcgggc
      721 cggtggcgcg ctcgggcggc ggctcgggcc gcacgcatcg cccctcgctc cggttccccc
      781 aggaggagac tccccccgtg cccaactggc gcccggagtt tcttgactac ctcttcacct
      841 ctgtatggac atcgaccgcg tttagcccca ccgaagcaat gccgcttgcc catcgcgtga
      901 aactgctcat ggcgatcgag tcggttgccg ggctcgtggt gttcgcgctc gccgtcggtc
      961 ggtcgatgaa tctgctctcg taggcggcac cgtctgtacg gcgctggccg accacgcgtc
     1021 cggctcgccg tcagggacga cccaggccgg gttgccgact cccgtggccc cggagccgtc
     1081 cggcgatcgc cccgcgaacc ccgctagacg atccggcgct gcgccgccca gtgggcgagc
     1141 tcgtggcggc tcgacagttg gagcttgcga agcaccgcgc tcacgtgcgt ctcgacggtc
     1201 ttgatcgaga tgaagagctt ctcccccacc tcgcggtagg agtagccgcg ggcgatcaac
     1261 cgcatcacct cttgctcacg cgccgtgagt ttgtctaggt cctcgtcgac catcgcgacg
     1321 tcgcccgctg cggcgccaaa ggcatccaga acgaaacccg caaggcgcgg ggagaagacc
     1381 gcgtcgccca acgcgacccg cttgacggca tccgtgaggt cgggcccgga gatggacttc
     1441 gtcacgtacc cgcgtgcgcc agcgcggacg accgcaacca cgtcctccgc cgcgtcggaa
     1501 accgacaggg cgaggaagcg aggcggctca ggcttcttca ggcaggcgtc gagcacatcg
     1561 agggcgccgc cgcccatccc cccgggaagg tgcacatcga ggatgacgac ctcgggccgg
     1621 gcgtcggcaa tcgcctggat ggccgactcc acgtccctgc cctcgcccac cacctccacg
     1681 ccctcgccga gcgactgctt gacgcccacg cgaatgacgt cgtgatcgtc aacgaccacg
     1741 accctgatcc ggtcgctcat cgcgccacct tcccgttctc cgcgcttctc ggcacggaaa
     1801 tggtcacctc ggtcgactgt cctcgcctcg acgctaccgc cgcgaagcct ccgtggcgct
     1861 cgacgcgtcc cacgatcgat tcgcggatgc cgaggcgatc cgcaggaatc ctcttcggat
     1921 tgaagccggg gccggagtcg cgcacgaaga cttggtatcg gtcgtcggag acttcggcgt
     1981 acacggagat cggcgcgacc ccatgcttcg ccgcgttcga ggcggcctcc ttcgcggcgg
     2041 cgaccgccgc gcgcaggcgc ttgtcggttg gcgcgtcccc gaccgagacc acgtcgatcg
     2101 cgaccgcgtg ggcgtcctca accgagctga tggccgcggc gagcgccgtc gcgacggatt
     2161 cggccgcggt gcgtcgatcc tggtagaggt aggtgcggag gtctcgctcc tgggcgcgcg
     2221 cgagtttcgc gacggtcgcc ggatcatccg agttcttgcg aatgagggtg agggtctgga
     2281 gcacggagtc gtggaggtgg gcggcaatct ccgctctctc gaactctcgg atccgctccg
     2341 atcgttcgac gcgaacctgg cgtgcgagcc gcaccaacca cggcccgaag agcagggcga
     2401 gtgccacgac cagcaccggg ggaaccatga cgaggcgcac gacgggctga tcggtgaact
     2461 cccagctcgc gaaccaccac atcgcgcaga aggcgagccc gatgccaacg aggaccctgg
     2521 cccagtggtc tcgcgcgaag aggccgacga ccacctctgg ccgacgcgcg tcctcgacgg
     2581 atgcgaggtc aagcgggctc cacacgagcg ccaggcccac gacgtaggcg acgagcgggg
     2641 ccacccacgc cgccggcctg ggccagccga aaagtgccga cgcgcgagcg aaggccgcca
     2701 tcaggaacgc cgccccgacg agcgcgcgcg gccacgctgc gttgggcacg tgccgccgaa
     2761 gcggaggcgc cagccgatcg ttcgcggtac tcgtcacgcc tccatcgtca cacacggccg
     2821 cgcccagcgg accgcccttt tcgcgatacc agggtgggat gagcgaagtt cagggtgcca
     2881 ctcagggtgg tacctcatgg cggacc
//
LOCUS       DASVKP010000200         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1384823, whole genome shotgun sequence.
ACCESSION   DASVKP010000200 DASVKP010000000
VERSION     DASVKP010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384823"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VF362_04000"
     CDS             complement(<1..345)
                     /locus_tag="VF362_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX7590123.1"
                     /translation="MPRERSIGVPHARTVASPLLDLPGAVEGSGIDAGVAWHYGDPAG
                     EQRALTEGRAFVDQSHLGVIAIAGPDRLTWLHALSTQDLNSLAPGFGVETMILSPQGR
                     IEHVAGIVDDGEA"
     gene            complement(355..915)
                     /locus_tag="VF362_04005"
     CDS             complement(355..915)
                     /locus_tag="VF362_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HEX7590124.1"
                     /translation="MEDPPRTANVTFAFPDGLAPEAYPLAWLVGRWTGAGVIDYPEIP
                     RLDVVQTVVFDQDGGPYLRYTSVISTLGLEGRNGSVWASEAGFWRVPSSAPEGIDLAS
                     GRHPVEALIADASGVLSLYLGTVGNGRIDLTTDLMAHTASGAEMAAATRMYGLVDGDL
                     LWAWDLAAFGHELRSYASARLARVQA"
     gene            complement(1041..1775)
                     /locus_tag="VF362_04010"
     CDS             complement(1041..1775)
                     /locus_tag="VF362_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590125.1"
                     /translation="MNPWTRATVAAASAALLAAGGLYGARWLALVVLLLSFVLAIGWP
                     ILTWTPHWIAGSAITLGAGALSVVGVLLGNDEPFLRHMVIALAASTVASLIVEVVYPS
                     PPGQVVTAVAGTVSGATVAASGAAWIAAARTPGAQDLVVAGAVTLAISAIASVLKTHM
                     GINIAIALTLSVAVGAGSGLVFSSIQWYGGALVGFLAASSFIAVHELTRREVANTSIW
                     AGIASGITPVTVAGALVYIGGRILVG"
     gene            1831..>2524
                     /locus_tag="VF362_04015"
     CDS             1831..>2524
                     /locus_tag="VF362_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7590126.1"
                     /translation="MADLLVLTASAQGASAVLPALDLLGHRVRVLLPEPSAMVDALDA
                     DVVVVDGRTDLASSRSTCRLVRATGIAVPLVLVLSEGGMSVVNADWGTDDIILAAASP
                     AELDARIRLLIGRAEAADPLDKASEFTSGELTVDTGGYTARLRGVPLDLTYKEFELLK
                     YLMQHPGRVYTRDQLLQEVWGFDYYGGTRTVDVHVRRLRAKLGAEHEQLIGTVRNVGY
                     RFDPAPTAAKTGV"
BASE COUNT          433 a          861 c          877 g          353 t
ORIGIN      
        1 ggcctccccg tcatcgacga tgccagcgac gtgctcgatg cgaccctgag ggctgaggat
       61 cattgtctcg accccgaagc ccggggcgag ggagttgagg tcttgcgtgg acagcgcgtg
      121 gagccacgtg aggcggtcgg ggcccgctat cgcgatgacg ccaaggtggg actggtcgac
      181 gaacgcgcgg ccctcggtga gcgcgcgctg ctcccccgcc gggtccccgt agtgccacgc
      241 cacaccagcg tcgatccccg agccctcgac cgcgccggga aggtccagga gcgggctcgc
      301 gacggtccgg gcgtggggca caccgatgga acgctcgcgg ggcatgggcg attcctaggc
      361 ttgcactcgc gcgaggcggg cggacgcgta ggagcgcagt tcgtgcccga acgccgcgag
      421 atcccacgcc cacagcaggt cgccgtccac cagaccgtac atccgggtgg cggccgccat
      481 ctcggcgccc gacgcggtgt gggccatcag atcggtggtc agatcgattc gcccgttgcc
      541 gacggtgccg aggtagagcg agaggacgcc cgacgcgtcg gcgatgaggg cctcaaccgg
      601 atgccgtccc gacgcgaggt cgatcccctc gggcgccgag gagggaacgc gccagaagcc
      661 cgcctcggat gcccacacgg acccgttccg tccctcgagg ccgagcgtcg aaatgaccga
      721 cgtgtagcga aggtacggcc cgccgtcctg atcaaagacg accgtctgga cgacgtcgag
      781 gcgtgggatc tcgggatagt cgatgacccc ggccccggtc catcggccga cgagccaggc
      841 aagcgggtac gcctcgggcg caagcccgtc gggaaacgcg aaggtcacgt tcgcggtccg
      901 cggaggatcc tccacgcgac gattccacac acccaagcgg tcgcgacgaa ggagctgatc
      961 cccagcacgg caaacgtgac ctcgagcgcg ctcatgcgcc catcctagcg gtggcgatac
     1021 gcggcccgga ggccctccgc ctagccgacg aggatgcgac ctccgatata caccagagcg
     1081 cccgccacgg tcacgggggt gatgcccgag gcgattcccg cccagatgct ggtgttggcg
     1141 acctcgcgcc tcgtgagttc gtgaacggcg ataaacgaac tcgccgcgag gaagccgacg
     1201 agggcgccgc cgtaccactg gatcgaactg aacaccagtc cgcttccggc tcccacggcg
     1261 acgctcagcg tgagggcaat ggcgatgttg attcccatgt gggtcttaag cacggacgcg
     1321 atcgcagaga tcgcgagagt aaccgcgccg gcgaccacaa gatcctgcgc accgggcgtc
     1381 cgggccgcgg cgatccacgc ggcgcccgac gcggcgacgg tcgcgccgga gacggtcccg
     1441 gcgacggcgg tgacgacttg ccccggcggg ctgggataga ccacctcgac gatgagggag
     1501 gcaaccgtgc tcgccgcgag ggcgatcacc atgtgacgaa ggaacggctc gtcgttgccg
     1561 agaagaaccc cgacgaccga aagcgcaccc gcgccaaggg tgatggcgct tcccgcgatc
     1621 cagtgcgggg tccaggtgag tatgggccag ccgatggcga ggacgaagga aagcaacagc
     1681 acgacaaggg cgagccaacg ggcgccgtac agtccacccg cggcaaggag cgcggccgac
     1741 gccgccgcaa ctgtcgcgcg ggtccacggg ttcatggcgc acagtgtgcc atggtttggc
     1801 ccggtagagt tgtggggacc ggaggtatcc atggccgacc tattggtgct gacggcgtcc
     1861 gcccagggcg cgtccgcggt gctacccgcc ctcgatctcc tcggccatcg cgttcgagtc
     1921 ctcttgcccg agccatcggc catggtcgac gccctcgacg ccgatgttgt ggtggtcgac
     1981 ggcaggacgg acctggcatc atcccgctcc acgtgtcgcc tcgtgcgtgc caccggcatc
     2041 gccgtaccgc tcgtccttgt cctgagcgag ggggggatgt cggtcgtcaa tgccgactgg
     2101 gggacggacg acatcatcct ggcggccgcc tctcccgccg agcttgatgc gcggatccgc
     2161 ctgctcattg gccgcgccga ggccgccgat ccgctcgaca aggcatcgga attcacgtcg
     2221 ggggaactga ccgtcgacac ggggggctac accgcgcggc tccgcggggt tccgctcgac
     2281 ctcacgtaca aggagttcga gctcctgaag tacctcatgc aacaccccgg acgggtctac
     2341 acccgcgacc aacttctcca agaggtgtgg ggcttcgact actacggcgg gacccgcacg
     2401 gttgacgtcc acgtgcggcg ccttcgcgcg aagctcggag ccgagcacga gcaactcatc
     2461 ggcacggttc gcaacgtcgg gtatcggttc gaccctgctc cgacggcggc gaagaccggc
     2521 gttg
//
LOCUS       DASVKP010000201         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_309451, whole genome shotgun sequence.
ACCESSION   DASVKP010000201 DASVKP010000000
VERSION     DASVKP010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309451"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VF362_04020"
     CDS             complement(<1..394)
                     /locus_tag="VF362_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590127.1"
                     /translation="MTSGPSLNLLVPASFAVLFLALWGAGYASIPWLLWNVRAVARRL
                     ADWLRARARFGGAFGRLEAYRSYLPLVLALAIGTVLIAEAADAFLDVASALATHDASV
                     QRTDAAVYDWVRLHRTPLLNAVFIGVTTG"
     gene            complement(391..588)
                     /locus_tag="VF362_04025"
     CDS             complement(391..588)
                     /locus_tag="VF362_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590128.1"
                     /translation="MRSTLVINLGTATNRHRGEVTPFNVPVLAGHALTLDRFAWDPAR
                     GGFALRTEKRSAQGAERWEAT"
     gene            complement(585..>1644)
                     /locus_tag="VF362_04030"
     CDS             complement(585..>1644)
                     /locus_tag="VF362_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEX7590129.1"
                     /translation="FARNPTDVSTMRQRLLDAGVAVLDSELDYGKIVQTGEPELDALA
                     SEIRGTVKVNALHVDHRKIVTIDGRVAYCGSANFGAQYLYHRPFDPEKDAHEEATARR
                     KAGQPEPWWTWHDGLARFEGPIVCDLDRVFRERWRLGGGADFRPLDAVIPAAPRGIRV
                     RSARVIKNEPSSRTNEIREAFRDRILAAERSIFIENPFLYHPAIVDALLAAKRARPLL
                     EVTLILPGVTWTPTTLTYDAQQYHYAAYIDAGVEIFEYQNHFVHLKLATFDLRWVIVG
                     SANLNFRSLEDDKDFEACVLIESEELAQSIDREVRVEDLRRSRRVAPEDVHGASVHAL
                     RARSRGPRTLLMIASREL"
BASE COUNT          268 a          545 c          578 g          253 t
ORIGIN      
        1 ctcccgtcgt gacgccgatg aacaccgcgt tcagtaacgg cgtccggtgg agacggaccc
       61 agtcgtagac cgcggcgtcg gtacgctgga cgctggcgtc gtgcgtggcg agcgccgacg
      121 cgacgtcgag gaacgcgtcg gcggcctcgg cgattaggac cgtcccgatc gcgagcgcca
      181 ggacaagggg caggtacgag cggtacgcct cgaggcgtcc gaacgctccg ccgaagcggg
      241 cgcgcgcgcg aagccagtcc gcgagccgcc tcgccacggc ccggacgttc cagaggagcc
      301 acggtatgga tgcgtacccg gcgccccaga gcgccaggaa caggacggcg aacgacgccg
      361 gaacgaggag gttcagcgag ggaccggacg tcacgtggcc tcccacctct ccgctccctg
      421 cgcagagcgc ttttccgtcc ggagggcgaa gcctccgcga gccggatccc aggcgaagcg
      481 gtccagggtg agcgcgtggc ccgcgagcac tgggacgttg aacggagtca cctcgccgcg
      541 gtgacgattc gtcgctgtgc cgaggttgat cacgagggtg gatctcacag ctcccgcgaa
      601 gcgatcatca agagcgtgcg cgggccccgc gagcgtgcgc gaagcgcatg cacggacgcg
      661 ccatggacgt cctccggcgc gacgcgcctc gaccggcgaa ggtcttcgac gcggacctca
      721 cggtcgatac tctgcgcaag ctcctcgctc tcgatgagca cgcaggcctc gaaatccttg
      781 tcgtcctcga ggctcctgaa attcagattc gcggagccca cgatgaccca tcgcaggtcg
      841 aatgtcgcga gcttcagatg gacgaagtgg ttctggtact cgaagatctc cacccccgcg
      901 tcaatgtacg ccgcgtagtg gtactgctgt gcgtcgtacg tcaacgtggt cggggtccac
      961 gtgaccccag ggaggatgag cgtcacctcg aggaggggac gcgcgcgctt ggccgcgagg
     1021 agcgcgtcca cgatcgcggg gtggtagagg aaggggttct cgatgaagat cgaacgttcc
     1081 gccgccagga tgcggtcgcg gaaggcctcg cggatctcgt tggtccgcga gctcggttcg
     1141 ttcttgatca cccgcgcgga ccggacgcgt atgccgcgcg gcgctgccgg gatcacggcg
     1201 tcgagaggcc ggaagtccgc tcctccgcca agccgccagc gctcgcggaa gacccggtcc
     1261 aggtcgcaga cgatcgggcc ctcgaaccgg gcgaggccgt cgtgccacgt ccaccagggc
     1321 tccggttgac ccgctttccg cctggcggtc gcctcctcgt gcgcgtcctt ctccgggtcg
     1381 aagggcctgt ggtacaggta ctgcgccccg aagttcgcgg agccgcagta cgcgacgcgg
     1441 ccgtcgatgg tcacgatctt ccggtgatcg acgtggaggg cattgacctt cacggtcccc
     1501 cgaatctcgc tcgcgagcgc gtcgagctcg ggctctcccg tctgaacgat cttgccgtag
     1561 tcgagctccg aatccaggac ggcgacgccg gcgtccaaga ggcgttgccg catcgtcgag
     1621 acgtcggtgg gattgcgggc gaag
//
LOCUS       DASVKP010000202         3765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_726407, whole genome shotgun sequence.
ACCESSION   DASVKP010000202 DASVKP010000000
VERSION     DASVKP010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726407"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VF362_04035"
     CDS             <1..769
                     /locus_tag="VF362_04035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7590130.1"
                     /translation="LAGAAVFVYCQFGLEPTSAALSLSPGAARFIFSLNAGAAGLLVA
                     VLAAVEHSRRRVSAADARRATTRAQFLANTDPLTGLANRRPVMDELERISAEGDYCVV
                     VADLDNFKELNDRFGHLCGDHVLAGIGTELGHHVRALDTVGRWGGEEFIFVLPGTSLG
                     DAMGFAERLRAAIEACSFECGGHVHRVTASFGVADGAGDGMSHRVVRRADDAMYGAKE
                     AGRNAVRSKPLSSVVAAPPTREIPLVHRPRARAQDSL"
     gene            882..2567
                     /gene="guaA"
                     /locus_tag="VF362_04040"
     CDS             882..2567
                     /gene="guaA"
                     /locus_tag="VF362_04040"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX7590131.1"
                     /translation="MTERVNPGERAMDDAAAGTAEARGALPRGGHPAAHHPVLVVDLG
                     AQYAQLIARRVREANVYSEIVPHSMSAADMLARDPAAIILSGGPSSVYEPGAPSVDAA
                     LFEAGIPVMGICYGFQLMARALGGTVAKTGLREFGRTTAAVAERGTFLAGSPAEQSVW
                     MSHGDAVHQAPPGFTVLATTEGSPVAAFEDAGRRMCGTQWHPEVRHTPLGQEALRAFL
                     FGIAGLEADWTNAGIIDEQVARIRAQVGDASVICGLSGGVDSAVAAALVQRAVGDQLT
                     CVFVDHGLLRAGEAEQVERDFVAATGVKLVVRDARARFLNALAGIREPEAKRKAIGRE
                     FIREFEGAARDIVAGSTVSNSGVANSGAADEARFLVQGTLYPDVVESGGGDGAASIKS
                     HHNVGGLPDDLNFQLVEPLRALFKDEVRAVGLELGLPEEIVWRQPFPGPGLAIRIIGE
                     VTQDRLEILRRADRIAREELSAAGLDREIWQCPVVLLADVRSVGVQGDGRTYGHPVVL
                     RPVSSEDAMTADWSRIPYETLARISTRITNEVAEVNRVVLDVTSKPPGTIEWE"
     gene            complement(2588..2803)
                     /locus_tag="VF362_04045"
     CDS             complement(2588..2803)
                     /locus_tag="VF362_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590132.1"
                     /translation="MSSRNRPIVYRPLSQRVTTMLWGVAVAGFGAGVIAHLQGYAFDM
                     ELLGIVVLAVLGAWILASAVVVIVRGR"
     gene            complement(2805..>3765)
                     /locus_tag="VF362_04050"
     CDS             complement(2805..>3765)
                     /locus_tag="VF362_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590133.1"
                     /translation="PLAIAIGVVVLVIVLATRANKPPSASGPMAIPPQRASAAAAPGS
                     ATFAATPAATPNKATRAAAANSAQESTTATPADGVPADAAAPPATPSAPPPPPRPRTP
                     GPGSAIYLLTLSAAVFAAAAIWLLDREGHLAVSPVVAWLAVAVVILGGAIILTGAAGR
                     RIGFLGFLATTFIIGWLIGLFLVPRVFAFADGGATITINSITHTIGNGHGWFDQDADG
                     VACGSYSLSPGEISGATRYVVEPGQTSVTVTSGTAVVVVPKDASLTFRSEGIVDGSLS
                     IEDRNVTCTLNRAQGDLYSILRTGETVTVNIASTDATIAIEEN"
BASE COUNT          562 a         1239 c         1397 g          567 t
ORIGIN      
        1 cctcgcaggt gcggccgtct tcgtctactg ccagttcggg ctggagccca cctctgcggc
       61 gctttcgctc tcgcccggcg ccgcacgctt catcttctcc ctcaatgcgg gcgcggccgg
      121 cctccttgtg gctgtgctcg ccgcggtcga gcacagccgt cgccgcgtct ccgcggccga
      181 tgctcgccgc gcgacaaccc gcgcgcagtt cctcgccaac accgatcccc taacgggcct
      241 ggcgaaccgg cgccccgtca tggacgaact cgagcgcatc agcgccgagg gcgactactg
      301 cgtcgtcgtc gcggatctcg acaacttcaa ggaactcaac gatcgcttcg gccacctgtg
      361 cggcgaccat gtcctcgcag gaatcgggac ggagctcggc caccacgtca gggcgctcga
      421 cacggtgggt cggtggggcg gggaggagtt catcttcgtg ctgccgggga cgagcctggg
      481 cgacgcgatg gggttcgccg agcggttgcg ggcggcgatc gaggcgtgct cgttcgagtg
      541 cggcggccac gtgcatcggg tgacggcctc gttcggagtc gcggacggcg cgggcgacgg
      601 gatgtcccac cgggtggtcc ggcgcgcgga cgacgccatg tatggggcca aggaagctgg
      661 ccgcaacgcg gttcgcagca agccgctgtc gagtgtcgtg gcggcaccgc cgactcgaga
      721 gatccccctg gttcaccggc cgagggccag ggcgcaggat agcctctgaa tcatgacgag
      781 cgaggcgccg agggagcggg aacgctctcg atcgatcgct ggcgtgatcg tcgtcgcgct
      841 cgtcgtggtg ctcgcgtccc ggtccgggcg ggagaatggg cgtgaccgaa cgggtgaacc
      901 caggggagcg cgcgatggat gacgctgcag caggcacggc cgaggcgcgc ggggcccttc
      961 ctcgcggcgg tcatcccgcg gcccaccatc ccgtcctcgt cgttgacctc ggggcccagt
     1021 acgcccaact gattgcgcgg cgcgttcgcg aggcgaacgt ctactccgag atcgtgccgc
     1081 attccatgag cgcggcggac atgctcgccc gcgatcccgc ggcgatcatc ctcagcggcg
     1141 gaccgtcctc cgtgtatgag ccgggtgccc ccagcgtgga tgccgcgctc ttcgaggcgg
     1201 gcattcccgt catgggcatc tgctacgggt tccaattgat ggcgcgggcc ctcggcggaa
     1261 cggtggcgaa gaccgggcta cgggagttcg ggcgcacgac ggcggcggtg gccgagcgcg
     1321 gcacgttcct tgctggaagc cccgcggagc agtcggtgtg gatgtcccac ggcgacgccg
     1381 tgcaccaggc acctccgggc ttcacggtgc tcgcgacgac cgagggttcc cccgtcgcgg
     1441 ccttcgagga tgccgggcgt cgcatgtgcg gcacccagtg gcaccccgag gttcgccaca
     1501 cgccgctcgg gcaagaggcg ctccgcgcgt tcctcttcgg cattgctggg cttgaggcgg
     1561 actggaccaa cgcgggcatc atcgacgagc aggtggcgcg catccgcgcg caggtcggcg
     1621 atgcgtcggt catctgtggg ctcagcggcg gggtggattc ggccgtcgcc gccgcgctcg
     1681 tgcagcgggc ggtcggcgac cagctgacct gcgtcttcgt cgatcacggc ctgcttaggg
     1741 cgggcgaggc ggagcaggtc gagcgggact tcgtggccgc gacgggggtg aagctcgtcg
     1801 tgcgcgacgc cagggcgcgc ttcctcaacg cgctggcggg gataagggag cccgaggcga
     1861 agcgcaaggc catcggcagg gagttcattc gagagtttga gggagcggcc cgcgacatcg
     1921 tcgcgggctc aactgtctca aactccggcg tcgcaaactc cggcgccgcg gatgaggcgc
     1981 gattcctggt ccaaggcacc ctctacccgg acgtcgtgga atcgggcggg ggcgacggcg
     2041 cggcgagcat caagagccac cacaacgtcg gcggcttgcc cgacgatctc aacttccaac
     2101 tcgtcgagcc gctccgcgcc ctcttcaagg acgaggtgcg cgcggtcgga ctcgagttgg
     2161 gcctccccga agagatcgtc tggcgccagc cattccccgg cccggggctc gcgatccgta
     2221 tcatcggcga ggtcactcag gacaggctcg agatcctgcg ccgcgccgac cgcatcgcga
     2281 gggaggagtt gagcgccgcc ggcctcgatc gcgagatctg gcagtgcccc gtcgtgcttc
     2341 tcgcggacgt ccgtagcgtc ggcgtccagg gcgatggccg cacgtacggc caccccgtgg
     2401 tcctgcggcc cgtgtcgagc gaggacgcga tgacggcgga ctggtcgcgc atcccctacg
     2461 agactctcgc gcgcatctcg acgcgcatca cgaacgaggt cgcggaggtc aatcgcgtgg
     2521 tccttgacgt gacgagcaag ccgccgggca ccatcgagtg ggagtagcgc ggctccccgc
     2581 tagggtgcta gcgaccccgg acgatcacga cgacggctga cgcgaggatc caggctccca
     2641 acaccgcgag cacgacgatc ccgagcagtt ccatgtcaaa cgcgtagccc tgcaggtggg
     2701 cgatgacgcc ggcgccgaat ccggcgaccg cgactcccca cagcatggtg gtgacgcgtt
     2761 gcgagagcgg tcggtacacg atgggacgat tcctggagct catgtcagtt ctcctcgatg
     2821 gcgatggtgg cgtcggtcga ggcgatgttc acggttaccg tttcgcccgt ccgaaggatg
     2881 gagtagaggt ccccctgggc gcggttgagc gtgcaggtga cgttgcggtc ctcgatcgat
     2941 agcgaaccgt cgacgatccc ctccgatcgg aaggtcaggg acgcgtcctt cgggaccacg
     3001 acaaccgcgg tccccgacgt cacggtcacg ctcgtctggc cgggctccac gacgtatcga
     3061 gtcgctcccg agatctcgcc cggcgagagg ctgtaggacc cgcatgcgac gccgtcggcg
     3121 tcttggtcga accagccgtg gccgttgccg atcgtgtggg tgatgctgtt gatggtgatg
     3181 gtcgcgccgc cgtcggcgaa ggcgaagacg cgcgggacca gaaacaggcc gatgagccag
     3241 ccgatgatga acgtcgtggc gaggaagccg aggaagccga tgcgtcggcc cgcagccccc
     3301 gtcaggatga tcgcaccccc gaggatgacg acggccaccg cgagccaggc gacgacgggc
     3361 gaaacggcaa ggtgaccttc cctatcgagc agccagatcg cggccgccgc gaagaccgcg
     3421 gcagagagcg tcagcaggta gatcgcggag ccggggcccg gcgtgcgggg tcggggcgga
     3481 gggggcgggg ccgaaggagt ggccgggggc gccgcagcat ccgcaggaac gccgtccgcc
     3541 ggtgtcgccg tggtcgactc ctgcgcggaa ttcgctgcgg ccgcgcgggt tgccttgttg
     3601 ggagttgcgg cgggggttgc ggcaaaggtc gcgctaccgg gggctgcggc ggccgaggcg
     3661 cgttgcggcg gaatcgccat gggcccggag gccgacggcg gcttgttggc gcgcgtggcg
     3721 aggacgatca cgagcacgac gacgccgatg gcgatggcga ggggg
//
LOCUS       DASVKP010000203         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1137807, whole genome shotgun sequence.
ACCESSION   DASVKP010000203 DASVKP010000000
VERSION     DASVKP010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137807"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="VF362_04055"
     CDS             complement(<1..1457)
                     /locus_tag="VF362_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7590134.1"
                     /translation="MPGTLIDPLVGRVVDGRYVVRELIARGGMASVYRATDKRLGRDI
                     ALKIMSAELLGQSADFSSRFRREARSAARLTHPGMVRVYDQGTDGDLNYLTMELIEGA
                     NLRQRLIAEGTLPLGEAMRIAEKLLDTIGAAHRLGLVHRDVKPENVLMDLDGEPKLAD
                     FGLARAVTEVTSTTTGTILGTVAYLGPELVSKGLSDARTDVYAMGILLYEMVTGRQPF
                     TGASAIEVASRHVHEDVPPPSSYVLWLPPEFDALIARMADRDPRKRPSDASEALALVR
                     QTRSMIDEPTLDRRAEPPSGSQALLSGSQPTAVLEPVPTGQTVTLPIGLGEDFADEDD
                     QWDGEIGPPTDDVPAKPHLAWWVGALAAAVVMLAAMGVWWYTGFGPGAYTTVPGVSGQ
                     AAAAAKATLDGAGLQVTMLDTFDDAVAPGIVIDTLPAGDTRIAKGGAVTVDVSKGPLM
                     VQIPKVVGVTITDAKARIAAALFPHPTVKEEPSDTV"
     gene            1570..1920
                     /locus_tag="VF362_04060"
     CDS             1570..1920
                     /locus_tag="VF362_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2175c family DNA-binding protein"
                     /protein_id="HEX7590135.1"
                     /translation="MDNDIEWLAVPDVADRLGIPASRVRDMLGERVLLAVRRGENNAL
                     AIPGAFLIEVDGVLQPLATLKGTLTLLGDAGFDDDAAMEWLLAPNDELGASPISELRL
                     GHRSHVRRVAQALF"
BASE COUNT          298 a          696 c          758 g          347 t
ORIGIN      
        1 acggtatcgc tcggctcctc cttgaccgtt ggatggggga agagcgcggc cgcgatccgg
       61 gccttggcgt cggtgatggt cacgccaacc accttgggaa tctgcaccat gagcggaccc
      121 ttggagacgt cgacggtcac cgcgcccccc ttggcgatcc gcgtgtcacc cgcggggagg
      181 gtgtcgatca cgattcccgg tgccacggcg tcgtcgaacg tgtcgagcat cgtgacttgg
      241 aggcccgcgc cgtccagcgt cgccttggcc gccgcggccg cctggccgct cacgccgggg
      301 acagtcgtgt acgcgcccgg tccgaagccc gtgtaccacc agactcccat cgcggcgagc
      361 atgacgacgg cggcggcgag ggcgccgacc caccatgcga ggtgtggctt ggcggggacg
      421 tcgtccgtcg gggggccgat ctcgccgtcc cactggtcgt cctcgtcggc gaagtcctcg
      481 ccgagcccga tgggaagggt gaccgtctgg cccgtgggaa cgggctcgag gacggcggtg
      541 ggttgggagc cgctgaggag ggcctgcgac ccggagggcg gctccgccct gcggtcgagc
      601 gtgggttcgt cgatcatgct tctggtctgg cgcacgagcg ccaacgcttc gctggcatcg
      661 gatgggcgct tgcgcgggtc gcgatcggcc attcgcgcga tgagggcgtc gaactccggg
      721 gggagccaga gcacgtagga cgacggggga ggaacgtcct cgtggacgtg gcgggaggcg
      781 acctcgatcg ccgaggcccc cgtgaacggc tggcgacccg tcaccatctc gtacaggagg
      841 atgcccatgg cgtacacgtc cgtgcgggcg tcgctcagtc ccttggacac gagttccggc
      901 ccgaggtagg cgaccgtccc caggatggtc cccgtggtgg tggacgtcac ctccgtcacc
      961 gcgcgcgcga gcccgaagtc cgcgagcttg ggttctccgt cgaggtccat cagcacgttc
     1021 tccggcttga cgtcgcggtg gacgaggccg aggcggtggg ccgcgccgat cgtgtcgagg
     1081 agcttctccg cgatgcgcat ggcctcgcca aggggcaggg tgccctccgc gatgaggcgc
     1141 tggcggaggt tcgccccctc gatcaactcc atcgtcaggt agttgagatc gccgtcggtg
     1201 ccctggtcat acaccctgac cattccggga tgcgtgagcc gggcggcgga tcgcgcctct
     1261 cgtcggaagc gggaagaaaa gtcggcggac tgccccagga gttcggcgct catgatcttc
     1321 agcgcgatat ccctgccaag gcgcttgtcc gtcgcgcgat agacggatgc catcccgccg
     1381 cgcgcgatca gttcgcggac gacgtaccga ccatcgacga cgcggccgac gagcggatcg
     1441 atcagggtcc ctggcacgga ctcattgtat ggggaggtcc ttccggaggg ctcaaggagg
     1501 gcgcgccatt ggcgcattgg tcgtgccgcc ttgcgaacgg caagcgggct tcctttgtag
     1561 gctagacgcg tggacaacga catcgagtgg ctcgccgttc cggatgttgc cgatcgactc
     1621 ggcattcccg cctcccgcgt gcgggacatg ctgggggagc gggtgcttct tgccgtgcgt
     1681 cggggcgaga acaacgcgct cgccatccca ggcgcgttcc tgatcgaggt ggacggggtc
     1741 ctccaacccc tcgccaccct gaagggaacc ctgacgctcc ttggcgatgc gggcttcgac
     1801 gacgacgcgg cgatggaatg gcttctcgcg cccaacgacg aactcggagc ctcgccgatt
     1861 tcggagcttc gcctagggca ccgctcgcac gtccggcggg tcgcccaggc gctgttctag
     1921 gagtctttac cttgtaggcg tcctaccggt cccgcgcgac ggcgtcagcc gcgatgccat
     1981 cgaggatgcg cgcgtagggc tgaacgagcc cgagcccggc aagcgccgag cgggcgcgcg
     2041 ccgccgtggt cgcgatctcc cgttcgacgg cgtcgatagc gcccgtggcc gcgatcgcc
//
LOCUS       DASVKP010000204         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1220993, whole genome shotgun sequence.
ACCESSION   DASVKP010000204 DASVKP010000000
VERSION     DASVKP010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220993"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VF362_04065"
     CDS             complement(<1..930)
                     /locus_tag="VF362_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7590136.1"
                     /translation="MIAVHDLEVRIGARTLLTPTSFSVGAGDRVGLVGRNGSGKTTLT
                     KILAGEGLPTAGTVSSRGKIGYLPQDPRTGDLDMLAIDRILSARELDVLVAKMWEAEE
                     GMASLDEVVRDKAIERYGVVEERFRAAGGYAAESEAARIAANLGLADATLRQPIGTLS
                     GGQRRRVELARILFGDSETLLLDEPTNHLDADSIVWLRDFLRSYSGGLVVISHDAGLL
                     RACANKVFHLDANRSELDQYSLGWDAYLQQREEDEKRRHRERDNAERKAEALIAQANK
                     MRAKATKAVAAQGMLRRAEKLVGSLEAERRHDKV"
     gene            1060..1713
                     /locus_tag="VF362_04070"
     CDS             1060..1713
                     /locus_tag="VF362_04070"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX7590137.1"
                     /translation="MPRIPRLNGATLALVAVFAALIAASTLWGGYPLGGGVPITLQTF
                     AVLLAGAALGAYRGAAAVLVYLVLGTAGAPIFSGHKAGPGVWTGTTAGFLAAFVVAAF
                     VTGWLAERLVARGRPSFSGFLGATVIGSFAVIGVIGWGYVALRAHLDFNATLGALTPF
                     LPGDAIKAFLAAGVATAVNAAYPQILAREAGAATPTTAAPTTAAGKAARPKASAAAN"
     gene            1716..2402
                     /locus_tag="VF362_04075"
     CDS             1716..2402
                     /locus_tag="VF362_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7590138.1"
                     /translation="MIEFQDASVVAPGTTVRILEATTLALAERRVAIIGANGSGKSTL
                     ARLINGLVLPSTGSVTVDGHDTRRDAAAVRRRVGFLFTDPSAQLIMPTAAEDVALSLR
                     RTVKGKGERASAAIAALESIGLGDKGSVSVHALSGGQRQLLALAGVLAVGPSIIVADE
                     PTTLLDLRWRAHVDALLAGLDQQVIEVTHDLDAASRADRVIVMDSGSVAFDGKPRAAV
                     AAYRRLMAGN"
     gene            2430..>2693
                     /locus_tag="VF362_04080"
     CDS             2430..>2693
                     /locus_tag="VF362_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590139.1"
                     /translation="MGLFGLYRERRTPLHRAPAWAKILGLVLAAAWLAWCTVPAWSVA
                     SALVAIALLASTLPPPAPTLRGVAPVLVIAAATGAYHLWQGDTR"
BASE COUNT          340 a         1010 c          918 g          425 t
ORIGIN      
        1 gaccttgtcg tggcgacgct ccgcctcgag cgatcccacg agtttctccg ccctgcgaag
       61 catcccctgg gcggcgacag ccttggtcgc cttcgcgcgc atcttgttcg cctgcgcgat
      121 gagcgcctcg gccttgcgct cggcgttgtc gcgctcgcgg tgacgacgct tctcgtcctc
      181 ctcccgttgc tggaggtagg cgtcccaccc gaggctgtac tggtcgagtt ccgagcggtt
      241 ggcgtcgagg tggaagacct tgttggcgca cgcgcgcagg aggccggcgt cgtgtgagat
      301 cacgacgagt cccccggagt acgagcggag gaagtcgcgg agccagacga tggaatcggc
      361 gtcgaggtgg ttggtcggct cgtcgaggag cagggtctcc gagtccccga agaggatgcg
      421 ggcgagctcg acgcgccgcc tctggccccc ggagagggtg ccgatcggct ggcgcagagt
      481 cgcgtcggcg agcccaaggt tcgcggcgat ccgggccgcc tcgctctccg cggcgtaccc
      541 gcccgccgcc cggaagcgct cctccaccac tccgtagcgc tcgatcgcct tgtcccgaac
      601 gacctcatcc agggaggcca ttccctcctc ggcctcccac atcttcgcca cgagcacgtc
      661 cagttcgcgc gcggacagga tgcggtcgat cgcgagcata tcgaggtcac ccgtgcgcgg
      721 atcctgaggg aggtagccga tcttgccccg cgaactcacc gttcccgcgg tcggaaggcc
      781 ctcgcccgcg aggatcttcg tcagcgtcgt cttgcccgag ccgtttcgcc cgacgagccc
      841 gacgcgatcc cccgccccca ccgagaagct cgtcggcgtc agcagggtgc gagccccgat
      901 gcgcacttcg aggtcgtgga cggcgatcac aggcaggctc caggtgggac tcggggattt
      961 gtagggttcc gacaacgttt cgggacccgg aggccgcgca cgccgccgtc cattgtatgg
     1021 ccggtatggt cgtccctatc ctcacctcaa aggagaagca tgcctcgcat ccctcgactg
     1081 aacggcgcga ccctcgcgct cgtcgccgtc ttcgccgccc tcatcgcggc gagcaccctc
     1141 tggggagggt atccgctggg aggcggcgtc cccatcaccc tccagacctt cgccgtgctc
     1201 ctcgccggag cggctctcgg tgcgtaccgg ggcgcggccg ccgtcctggt gtacctcgtc
     1261 ctcggcacgg ccggggcacc catcttctcg ggccacaagg ccggacccgg cgtctggacc
     1321 ggcacgacgg cggggttcct cgcggcgttc gtcgtcgcgg cgttcgtgac gggctggctc
     1381 gccgagcgtc tcgtggcgcg cgggcggccc tccttctcgg gcttcctcgg cgcgaccgtc
     1441 atcggctcgt tcgcggtcat cggcgtgatc ggctggggct atgtcgcgct gcgcgcgcac
     1501 ctcgacttca acgcgacgct cggggcgctc acgccgttcc tgccgggaga cgccatcaag
     1561 gcgttcctcg ccgccggagt cgcgacggcg gtcaacgccg cctatccgca gatcctcgcg
     1621 cgtgaggccg gcgccgccac gcccaccacc gccgcgccca ccaccgccgc gggcaaggcg
     1681 gccaggccca aggcttcggc ggccgccaac tagccatgat cgagttccag gatgcgagcg
     1741 tcgtcgcccc cggcacgacc gttcgcatcc tggaagcgac cacgctggcc ctcgcggagc
     1801 gtcgcgtcgc gatcatcggc gcgaacggct cggggaagtc caccctcgcc cggctcatca
     1861 acgggctcgt gctcccgtcg acgggctcgg tcacggtcga cggccacgac acgcgccggg
     1921 acgccgccgc ggttcgccga agggtcgggt tcctcttcac cgacccctcg gcgcaactga
     1981 tcatgcccac ggcggccgag gacgtcgccc tgtcccttcg gcggacggtc aagggcaagg
     2041 gcgagagggc cagcgcggcg atcgcggcac tcgagtccat cgggctcggg gacaagggct
     2101 cggtgtcggt tcacgccctg agcggcggcc agcgccagct tctcgccctc gcgggggtcc
     2161 tggcggtcgg gccgtcgatc atcgtcgccg acgagccgac gacgctactc gaccttcggt
     2221 ggcgcgccca cgtggacgcg ttgctcgccg ggctcgacca gcaggtgatc gaggtgacgc
     2281 acgacctcga cgcggcctcc cgcgccgacc gcgtgatcgt gatggattcg ggctcggtcg
     2341 cgttcgacgg caagccccgc gccgcggtcg cggcctaccg gcgcctcatg gctggcaact
     2401 gatggccggc gactgatggc cagcgactag tgggtctctt cgggctctac cgcgagcggc
     2461 gcaccccgct ccaccgagcg cccgcctggg cgaagatact cggcctcgtc ctcgccgccg
     2521 cctggctcgc gtggtgcacc gttcccgcct ggagcgtcgc gagcgccctc gtcgcgatcg
     2581 cgctcctggc cagcaccctg ccgccacccg cgccgacgct ccgcggcgtc gcccccgtgc
     2641 tcgtgattgc ggccgcgacc ggcgcctacc acctgtggca gggcgacacg agg
//
LOCUS       DASVKP010000205         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_559340, whole genome shotgun sequence.
ACCESSION   DASVKP010000205 DASVKP010000000
VERSION     DASVKP010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559340"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VF362_04085"
     CDS             <1..1048
                     /locus_tag="VF362_04085"
                     /inference="COORDINATES: protein motif:HMM:NF015926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HEX7590140.1"
                     /translation="IRDIDGTLTSLRAVVTTPDCVGDLAAFRERLAPIEAQATVFRDT
                     MAAAKAEARALALKEREEIVARAEAIAAKGEDAIRWKDDTAELRQLLDTWKTAQKSGA
                     RLGKDVEKELWQRFAHARSAFERTRKHHFADLDKVNTDVASHKEALTAKAESLATSRD
                     WDATSRAFRDLMGEWKNAGRGRRSADDALWTRFRAAQDAFFEAKRIATEATVGIMTAN
                     LAAKEAAVKEAEAILPIRDLPLAKSTLRGAQDRFEAAGEVPKPDAIRLARRLSALEKA
                     VREADGAVWERKNPELEARASGAAQQLVAAIADLDHQIAAATKSGDAKAVASLTESRD
                     ARQSWLDQIQATVS"
     gene            1134..1754
                     /locus_tag="VF362_04090"
     CDS             1134..1754
                     /locus_tag="VF362_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590141.1"
                     /translation="MIPVSAQEVGPVALARMLRDGVLAPLAPRHGMPRDVPESSLARA
                     LCLSPVVPAHTVVSGLAGLWVRHGGAAPSVADLVGARGLHRAKPGAEARGWTLVFHSG
                     GAATGPRTEFGGIVVASAERCAADALRWRDLATAIEVVFLAVRAGSVEPAAIEALVVR
                     DDPRGHGAARARYAWGALAATLGPSPTANPEGGVCVCPSAPGGECP"
     gene            complement(1726..>2786)
                     /locus_tag="VF362_04095"
     CDS             complement(1726..>2786)
                     /locus_tag="VF362_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX7590142.1"
                     /translation="ALRGEIGPAEVYAFTPGGKLLALPEGSTPVDFAYAVHTEVGHRT
                     IGAKVNGRLVPLDSRVANGDTVEILTSSGENAAPKRDWLEFVASTRARNKIRHWFTRE
                     RREESIETGRDMLARAMRRQHLPMQRLMTRQSLLVLCRESHYEDVEALYAAIGEHREQ
                     ASDVVARIVQAVGGAAGAEEDIAESTRPGVRRPRRVQGDPGITVHGLTDVVVKLAKCC
                     TPVPGDVIQGFVTRGSGVSVHRIDCDNLAVLREQPERLVDVEWTGNADATFLVQIEVE
                     ALDRARLLSDITHLLSDHHLNILTANLSTTKDRVALSNFTFEMADPSHLGAVLADVRK
                     IDGVYDVRRLTGTRRPEH"
BASE COUNT          399 a         1001 c          987 g          399 t
ORIGIN      
        1 gatccgcgac attgacggga cgctcacgtc tcttcgtgcc gtcgtcacca ccccggactg
       61 cgtgggcgac cttgccgcct tccgcgagcg cctggcaccc atcgaggcgc aggcgaccgt
      121 atttcgggac acgatggccg cggcgaaggc cgaggctcgt gccctcgcgc tcaaggaacg
      181 tgaggagatc gttgccaggg ctgaggcgat cgccgccaag ggcgaggatg ccatccgctg
      241 gaaggatgac acggccgagt tgcgacagct cctcgacacg tggaagacgg cgcagaagag
      301 cggcgcccgc ctcggcaagg acgtagagaa ggagctgtgg cagcgcttcg cgcacgcgcg
      361 ttccgccttc gagcgcaccc gcaagcatca cttcgccgat ctcgacaagg tcaacaccga
      421 cgtcgcctcc cacaaggagg ccctcaccgc caaggcggaa agcctcgcga cctcgcgcga
      481 ctgggacgcc acctcgcggg ccttccgcga cctcatgggc gagtggaaga acgcgggccg
      541 cggccgtcgg tccgccgatg acgccctgtg gacgcgattc cgcgccgccc aggacgcctt
      601 cttcgaggcg aagcgcatcg ccaccgaggc gaccgtcggg atcatgacgg cgaacctcgc
      661 ggccaaggag gccgcggtga aggaggcgga agcgattctg ccgatccgcg accttcccct
      721 cgcgaagtcc accctccggg gtgcgcagga ccgattcgag gccgcgggcg aggtccccaa
      781 acccgatgcc atccgcctcg cccgacgcct cagcgcgctc gagaaggccg tgcgcgaggc
      841 cgacggagcc gtgtgggagc ggaagaaccc cgaactcgag gcacgcgcga gcggggcggc
      901 gcaacaactc gtcgccgcga tcgccgacct cgaccaccag atcgcggccg cgaccaagtc
      961 cggcgacgcg aaggcggtcg ccagcctcac ggagtcgcgc gacgcccgtc agtcatggct
     1021 cgaccaaatc caggcgaccg tctcctagcc cccggcgctc tccccaggcc agcgttctcc
     1081 ccagttcggc ggcgccgctc gccctcgtcc gccacttgcg ccaccctggg ggcgtgatcc
     1141 ccgtctctgc ccaggaggtt ggccccgtcg cgctcgcgcg aatgctccgg gacggggtcc
     1201 tcgcgccgct cgccccgcgc cacggaatgc cgcgcgacgt ccccgaatcg tcactcgcgc
     1261 gggcgctctg cctctccccc gtcgtgcccg cgcacacagt cgtctccggc ctcgccgggc
     1321 tctgggtgcg ccacgggggc gccgcgccct ctgtggccga tctcgtgggc gccaggggcc
     1381 tccaccgagc gaagcccggg gccgaggccc gcggctggac gctcgtcttc cactcgggcg
     1441 gggccgcgac ggggccgcgc acggaattcg gggggatcgt cgtcgcgagc gcggagcggt
     1501 gcgccgccga cgccctgcgc tggcgggacc tcgcgacggc catcgaggtc gtcttccttg
     1561 ccgttcgggc cgggagcgtc gagcccgccg cgatcgaggc tctcgtggtg cgggacgatc
     1621 cgcgtggtca cggcgcggcg cgcgcccggt acgcgtgggg cgccctggcc gcgacgctcg
     1681 gtccctcccc aacggcgaac ccggaaggcg gcgtgtgtgt ttgccctagt gctccgggcg
     1741 gcgagtgccc gtgaggcgcc ggacgtcgta gacgccgtcg atcttgcgaa cgtccgcgag
     1801 cacggcgccc aggtgcgagg ggtccgccat ctcgaaggtg aagttcgaca gtgcgacgcg
     1861 atccttcgtc gtcgacaggt tggccgtgag gatgttcagg tgatggtcgg agaggaggtg
     1921 ggtgatgtcg ctcagcaggc gggcccgatc gagggcctcg acctcgatct gcacgaggaa
     1981 cgtggcgtcc gcgttgccgg tccattcgac gtcgacgagc cgctcgggct gctcgcggag
     2041 caccgcgagg ttgtcacaat cgatccggtg gacgctgacg ccggagccgc gtgtgacgaa
     2101 gccctggatc acgtccccgg ggaccggggt gcagcacttg gccagtttga cgacaacgtc
     2161 cgtgagcccg tggacggtga ttcccgggtc cccctggacc ctgcgcgggc gccgcacgcc
     2221 gggcctggtg ctctcggcga tgtcctcctc tgcgcccgcc gcgccgccga cggcctggac
     2281 aatacgggcg acgacgtcgg acgcctgctc cctgtgctct ccgatcgcgg cgtacagggc
     2341 ctcaacgtcc tcgtagtgcg actcccggca caagacgagg agtgactgtc gggtcatgag
     2401 acgttgcatg gggaggtgct gcctccgcat cgcgcgggcc agcatgtcgc ggcccgtttc
     2461 gatgctctcc tcgcgccgct cgcgcgtgaa ccagtggcgg atcttgttgc gcgcacgagt
     2521 cgacgcgacg aactcgagcc agtcccgctt gggcgcggcg ttctcgccgg aagaggtcag
     2581 gatctcgacg gtgtcaccgt tggccacgcg cgagtcgagc gggaccaggc gcccgttgac
     2641 cttcgcgccg atcgtccggt gcccgacctc ggtgtggacc gcataggcga agtcgacggg
     2701 agtcgaaccc tccgggaggg cgaggagctt gccccccggc gtgaaggcgt agacctccgc
     2761 gggcccaatc tcgccgcgca gcgcgt
//
LOCUS       DASVKP010000206         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1544432, whole genome shotgun sequence.
ACCESSION   DASVKP010000206 DASVKP010000000
VERSION     DASVKP010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544432"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /gene="nusB"
                     /locus_tag="VF362_04100"
     CDS             <1..407
                     /gene="nusB"
                     /locus_tag="VF362_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX7590143.1"
                     /translation="EADQRGLNVLLALDARIAEPGVATPLPEYAIQIVRGVVDHWNDI
                     ETAMAAHSQGWEISRMPAVDRAALRLATWEILFNDDVPAAVAIDQAVDLVTDLSTDES
                     PGFVNGVLGAIAASAHGASDAPPGGKPGDPLD"
     gene            478..1017
                     /gene="pyrR"
                     /locus_tag="VF362_04105"
     CDS             478..1017
                     /gene="pyrR"
                     /locus_tag="VF362_04105"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX7590144.1"
                     /translation="MAAPDIARALTRIAHEILERNGGADDLVLLGIPTRGLPLARRLA
                     ERIAVVEPAFDAQARSGGIDITMYRDDLRRQPTRPIGTTTLPPAGIDGAIVVLVDDVL
                     HTGRTIRAALDAIGDLGRPRSVQLAVLVDRGHRELPIRADFVGKNLPTSRSERVSVLL
                     DEIDHQEEVRIERVEGEVE"
     gene            1014..1973
                     /locus_tag="VF362_04110"
     CDS             1014..1973
                     /locus_tag="VF362_04110"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX7590145.1"
                     /translation="MKHLLTTRGLPRDEAIRILDTAGEMAKTQEREIRKLPTLRGKTV
                     INLFFEDSTRTRISFEAAAKRLSADVINFSAKGSSVAKGESLKDTAQTLQAMGADGVV
                     IRHWASGAAERLATSGWTRGAVVNAGDGTHEHPTQALLDAYTIRRHLADGEPGVGLDG
                     LTVAIVGDILHSRVARSDAWLLTTLGARVILVAPPTLVPLGVETWPVEVVHSLDEALE
                     KHDLDAIMMLRIQLERMTGGGAAFFPSPAEYSRLYGLNGTRLARLPKRTIVMHPGPMN
                     RGLEISAEAADSPHSVVVEQVANGVSVRMAVLYLLLAGEEVAS"
     gene            2039..>2383
                     /locus_tag="VF362_04115"
     CDS             2039..>2383
                     /locus_tag="VF362_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7590146.1"
                     /translation="MLDKGAIVSLSAADAPKGATVIDGAGLVALPGLVDLHTHLREPG
                     REDAETIETGSRAAAAGGYVAVHAMANTTPVADTAGVVEQVWHRGREIGLVDVFPVGA
                     VTVGLAGEHLAEL"
BASE COUNT          374 a          834 c          817 g          358 t
ORIGIN      
        1 tcgaggccga ccagcgcggg ctgaacgtcc tcctggccct cgacgcccgc attgcggaac
       61 ccggcgtcgc gacgcccctg cccgagtacg ccattcagat cgtgcgtgga gtggtcgatc
      121 actggaacga catcgagacg gcgatggcgg cgcactctca ggggtgggag atttcgcgca
      181 tgcccgccgt cgaccgtgcc gcgcttcgcc tcgccacgtg ggagatcctc ttcaacgacg
      241 acgtcccggc cgccgtcgcc atcgatcagg cggtcgatct cgtcaccgat ctgagcaccg
      301 acgagtcgcc gggcttcgtc aatggcgtcc tcggcgccat cgcggcgtcg gctcacggcg
      361 cgtcggatgc ccccccgggg ggcaagccgg gcgatccgct agactgacgc gagcaggcat
      421 cctttaaggt ccgtccagag agacggggaa ggaggtcgcc aggtgactgg tgccgtcatg
      481 gccgcgcccg atattgccag ggcgctcacg cgcattgccc acgagatact cgaacgcaac
      541 ggcggcgcgg acgatctcgt gctcctcgga atccccacgc gggggcttcc tctcgcgcgc
      601 cgcctcgcgg aacggatcgc ggtcgtcgag ccggcgttcg acgcccaggc ccgctcgggc
      661 ggcatcgaca tcaccatgta ccgcgacgat ctccgccgcc agccgacgcg tccgatcggc
      721 accaccacgc tgcctcccgc aggaatcgac ggcgcgatcg tcgttctggt ggatgacgtc
      781 ctgcatacgg ggcggacgat tcgcgcggcg ctcgacgcga ttggcgacct tggaaggccg
      841 cgctcggtgc aactggccgt gctcgtcgat cggggccacc gtgaactgcc gatacgcgcc
      901 gacttcgtcg gcaagaacct tcccacgtcg cgctccgagc gtgtgagcgt gctcctcgac
      961 gagatcgacc accaggagga agttcgcatc gagcgagtgg agggggaggt cgagtgaagc
     1021 acctgctgac gacgcggggc cttccgaggg acgaggccat tcggatcctc gacaccgcgg
     1081 gcgagatggc gaagacccag gagcgggaga tccgcaaact ccccacgctt cgcgggaaga
     1141 cggtcatcaa cctgttcttc gaggactcga cgaggacccg catcagcttc gaggccgcgg
     1201 caaaacgcct gtcggccgac gtgatcaact tctcggcgaa gggatcgagc gtcgccaagg
     1261 gcgagtcact caaggacacc gcacagacgc tccaggccat gggcgccgac ggggtggtga
     1321 tccgccactg ggcatccggt gccgcggagc ggctcgcgac ctcgggctgg actcgcggag
     1381 cggtggtcaa tgcgggcgac ggcacccacg agcaccccac gcaggcgctc ctcgacgcgt
     1441 acacgattcg ccgtcacctc gcggacgggg agcccggagt cggcctcgac ggcctcaccg
     1501 tggcgatcgt tggcgacatc ctccactcgc gcgtcgcgcg ctcggatgcg tggctcctga
     1561 cgacgctcgg ggcgcgggtc atcctggtcg ccccgccaac gctcgtgccc ctcggcgtcg
     1621 agacctggcc ggtcgaggtg gtccactcgc tggacgaggc gctcgagaaa cacgacctcg
     1681 acgcgatcat gatgctgcgg atccaactcg agcggatgac cggcggggga gcggcgttct
     1741 tcccgtctcc tgccgagtac tcccgcctgt acgggctcaa cggcacgcgc ctcgcgcgcc
     1801 tgcccaagcg gaccatcgtc atgcaccccg gtcccatgaa ccgcggcctg gagatctccg
     1861 cggaagccgc ggactcgccg cactccgtcg tcgtcgagca ggtcgcgaac ggcgtttcgg
     1921 tgcgcatggc cgtcctgtat ttgctgctcg ccggagagga ggtcgcctcg tgacctcggc
     1981 cgtggggccc atcgtcatca cgggtgtttc gctctacgga gacactcccg ccgatctcgt
     2041 gctcgacaag ggcgcgatcg tctcgctgag cgcggccgat gcgccgaagg gcgccacggt
     2101 catcgacggc gcgggcctag tcgcccttcc cgggctggtg gaccttcaca cgcacctacg
     2161 cgaaccgggc cgcgaggacg ccgagacgat cgagacgggg tcgcgcgcgg ccgcggccgg
     2221 cggctacgtc gcggtccacg ccatggcgaa cacgactccg gtagctgaca cggcgggcgt
     2281 cgtcgaacag gtgtggcaca ggggccgaga gattggtctc gtcgacgtct tcccggtcgg
     2341 cgccgtcacg gtgggactcg cgggggagca cctcgcagaa ctc
//
LOCUS       DASVKP010000207         4724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_644051, whole genome shotgun sequence.
ACCESSION   DASVKP010000207 DASVKP010000000
VERSION     DASVKP010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644051"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..618)
                     /locus_tag="VF362_04120"
     CDS             complement(106..618)
                     /locus_tag="VF362_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX7590147.1"
                     /translation="MNDLIMTVNGWVATDPSQHVGPTGARLTSFRLASTSRFFDRDKG
                     EWTDGRTEWFTVKVFRNAAITVANSIKKGQPVTVHGRFRTNEWEGEGGTRTDLVIDAT
                     SVGHDLTRGTAEFTRAFGDASLAGEDGAADAVLEGTAEGDDSAERDADGKADANDEAE
                     EIDEPLALSA"
     gene            complement(792..2111)
                     /locus_tag="VF362_04125"
     CDS             complement(792..2111)
                     /locus_tag="VF362_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX7590148.1"
                     /translation="MSVSFTHLDPPSETTYLRERVTRLRSALEWAGDALTPEVKRSIG
                     DTVKRCEERLALGVDTTIVALAGGTGSGKSSLFNAIARMDFALAGVSRPTTAQVSAAT
                     WGGRADALLDWLGVAPDRRLVCETALDTEVEAQFRGMVLLDLPDHDSVQAENRAIVDR
                     IVPMADLLVWVVDPQKYADHALHSRYLSAVAESGAPSAVILNHTDRLTRKDADAILFD
                     IRRLLAEKGIESVPVLLTSATTGAGIDSVRSVLANVARRRSVAAESVRAELVAAGRLL
                     ADALAKDSDPALPEPAELANELAKAAGVDVRARAAGEVAAGRQAAYPGLAILTAAAVS
                     PLREAWIDAATDGLPRTWRLVVSKAIAKPAAIAELVNDALGVVRWPEVKPAKRRFGRT
                     SSAPSMEAAILGLGREAVLAAIREDVVEPTQMVHQAYRALDELTQLG"
     gene            complement(2108..3697)
                     /locus_tag="VF362_04130"
     CDS             complement(2108..3697)
                     /locus_tag="VF362_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEX7590149.1"
                     /translation="MTIRPVRSWGRPGALLDALRATRSAVGSVDLPFESPEAERAKAL
                     VHDILEQLDQHLIPRVTEGSVPAVVVVAGSTGSGKSTIVNALLGDDVTAAGVLRPTTK
                     VPHLFHHPADGVLLSSATRRATSRASDAVPRGLVLVDSPDIDSLVGENREIARELLDA
                     ADLWIFVTTAARYGDAVPWEVLRAGASRGASIAIVLSRVTADVAAHVRRDLVERLKAE
                     GLESLPLFVIPEEPEGLTALPEDVVQGLRRWLDTVAAKSATTIVERTMLGATQALKEW
                     LEQLAEFMDNQAGAVKQARGEVRRAAARAEQEGGEYWFREIAVGPIASLWSRGADPGG
                     PLFRISSSPWTKRRATAVTRERALGEIRGELLRAVEGTLSFTAAQASARMVEGLTLDA
                     TGVGAWILAQRDPTEAAGARARHAREAALGWMERCSEIAGTLPAAPGVTQYVGADGLA
                     TVFASAALGVPQARQALALLVGSGLQEHFDQARSHLAAARRYCISREALDMIAPSDLP
                     SLAPDASSAIRLRRAELRGLL"
     gene            3792..3868
                     /locus_tag="VF362_04135"
     tRNA            3792..3868
                     /locus_tag="VF362_04135"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3826..3828,aa:Arg,seq:tct)
     gene            complement(3916..>4724)
                     /locus_tag="VF362_04140"
     CDS             complement(3916..>4724)
                     /locus_tag="VF362_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7590150.1"
                     /translation="AGIVIGDEPAPFKREIERFHGALASLGEIVAFLALGLTVDLGVV
                     SRGDVWIPGLILAVVVALVIRPVLVGLCLIPSRLPRSQSAFVLFAGLKGAVPILLGSF
                     LLVAHVPDAHRLHGIVVVVVVFSVVVQGSLTPAVARWLRIPVRVIEPQPWALGVRLRE
                     EPDGVHRLTVVKGSPADGRSIQDLTEVSDEAWVSMVVRAGQLVPVRPSTTLRAGDDVV
                     IFAGPETRAELVRAFGLEEVEGPHTGSVAAWGSRGRGGRRDRRRPKADAS"
BASE COUNT          689 a         1734 c         1638 g          663 t
ORIGIN      
        1 gcgtcgcgca tgaagagggg ccccgacgcc gtggcgtcgg ggcccgcgaa cggtcggctc
       61 tctggggcca ctctcctatt gccttcgttc tatcgcttct tcgacctagg ccgaaagggc
      121 caggggctcg tcgatctcct cggcctcgtc gtttgcgtcg gctttaccat ccgcgtcccg
      181 ctcggccgaa tcgtcgcctt ccgcagtccc ctccaaaacc gcgtctgcag ctccgtcctc
      241 cccggcgagc gaggcatccc cgaacgcgcg cgtgaactcc gcggtgccgc gggtcaggtc
      301 gtggccgacg ctcgtcgcgt cgatcacgag gtccgtgcgg gtgccgccct cgccctccca
      361 ctcgttcgtg cggaagcgcc cgtgcaccgt cacgggctgg cccttcttga tggagttcgc
      421 gaccgtgatg gccgcgttcc ggaacacctt gacggtgaac cactccgtgc gtccgtccgt
      481 ccactcccct ttgtcgcggt cgaagaatcg ggacgtcgag gcgagccgga aactcgtgag
      541 gcgggccccc gtgggcccga cgtgctggct gggatcggtc gccacccagc cgttgacggt
      601 catgatgagg tcgttcatgc caccctcctt gggttgtgcc gcgcggtgcg gctcccgccg
      661 agcgtgccgc gcccgcctcg cccgcgactg acgcacgttc cgaagggagg caacggcgcc
      721 gcccacggac cctggggaca tcgccgagcc gcgcgaagag gcgccgcccg cgggtgggac
      781 gaggggcagg atcaccccag ctgcgtgagt tcgtcaagcg cgcggtacgc ctggtgaacc
      841 atctgcgtgg gctcgacgac gtcctcgcga atcgcggcga gaacggcctc acgcccgagc
      901 ccaaggatcg cggcctccat cgatggcgca gaggaggtgc ggccgaagcg gcgcttcgcg
      961 ggcttgacct cgggccaccg cacgactccc agcgcgtcgt ttaccagttc cgcgatcgca
     1021 gctggcttcg cgatcgcctt gctcaccacc agccgccacg ttcgaggcag gccgtcggtc
     1081 gcggcgtcga tccacgcctc gcgaagcggc gacacggccg cggcggtcag gatggcaagc
     1141 ccgggatagg cggcctgacg ccccgcggcg acctcgcccg ccgcacgcgc cctgacgtcc
     1201 acgccggcgg ccttggcgag ctcgttcgcg agttccgcgg gctcgggaag cgcgggatcg
     1261 gagtccttgg cgagcgcgtc cgccagcaac cgtcccgccg cgacgagctc ggcacgtacg
     1321 gattcggccg cgacggagcg gcggcgcgcg acgtttgcga gcacgctcct cacgctgtcg
     1381 atccccgctc ccgtcgtcgc ggaggtcagc aggaccggca cggactcgat gcccttctcg
     1441 gcgagcagcc gacggatgtc gaagaggatc gcgtccgcgt ccttgcgggt gaggcgatcc
     1501 gtgtggttga ggatgacggc cgagggcgcg ccggactccg ctaccgcgga aaggtagcgc
     1561 gagtgcagcg cgtgatcggc gtacttctgg ggatccacca cccacaccag aaggtccgcc
     1621 atcgggacga tgcgatcgac gatcgccctg ttctcggcct ggacggagtc gtggtcggga
     1681 aggtccaaca gcaccatccc gcgaaactgc gcctccacct cggtgtcgag cgccgtctcg
     1741 catacgagcc gccgatcggg ggccaccccg agccaatcga gcaacgcgtc cgcgcgtccg
     1801 ccccacgtcg ccgcgctcac ctgcgctgtc gtcgggcgcg acaccccggc cagggcgaag
     1861 tccatgcgcg cgatcgcgtt gaacagcgag gacttgcccg aacccgtacc ccccgcgagc
     1921 gcgacgatcg tggtgtcgac tccgagcgca agcctctcct cgcaccgctt cacggtgtcg
     1981 ccgatggacc gtttcacttc gggggtgagg gcgtcgcccg cccactcgag cgcggacctg
     2041 agccgcgtga cgcgctcccg aaggtaggtg gtctcgctcg gcggatcgag gtgcgtgaac
     2101 gacacgctca cagaaggccc ctcagttccg cgcgccgcag gcggatggcc gacgacgcgt
     2161 ccggagcgag ggagggcagg tcgctcgggg cgatcatgtc gagcgcctcg cggctgatgc
     2221 agtaccggcg ggcggcggcg aggtgcgagc gcgcctggtc gaagtgctcc tgaagccccg
     2281 agccaacgag caacgcgagt gcctggcggg cctggggcac gccgagcgcg gccgacgcga
     2341 acaccgtcgc gaggccgtcc gcgccgacgt actgcgtgac gccgggggcc gccggcagcg
     2401 tccccgcgat ctcggagcac cgctccatcc aaccgagggc ggcctcgcgg gcgtggcgcg
     2461 cgcgagcccc ggccgcctcc gtggggtcgc gctgagcgag aatccatgcg cccacgccgg
     2521 tggcgtcgag cgtcagcccc tcgaccatgc gggccgacgc ctgcgccgcg gtgaacgaga
     2581 gcgttccctc caccgcgcgc aggagttccc cacggatctc gccgagggcc cgctcgcgcg
     2641 tcaccgccgt ggcccttctc ttggtccagg ggctcgaact gatccggaag agcgggccgc
     2701 cgggatcggc gccacgcgac cacagcgacg cgatcgggcc gaccgcgatc tcgcggaacc
     2761 agtactcgcc gccctcctgc tcggcgcggg cggcggcccg tcgtacctcg cccctcgcct
     2821 gcttgaccgc gcccgcctgg ttgtccatga actccgcgag ctgctcgagc cactccttga
     2881 gcgcctgcgt cgcccccagc atcgtgcgct ccacgatggt cgtcgcgctc ttggccgcga
     2941 ccgtgtcgag ccagcgccgg agtccctgga cgacgtcctc gggcagggcc gtcagtccct
     3001 cgggctcctc cggaatgacg aacagcggca gcgactccag gccctcggcc ttgagccgct
     3061 ccacgaggtc gcggcggacg tgcgcggcga cgtcggcggt cacgcggctg aggacgatgg
     3121 cgatcgacgc ccccctgcta gcccccgcgc gaagcacctc ccacggcacc gcgtcgccgt
     3181 accgggccgc cgtcgtcacg aagatccaca ggtccgcggc gtccaggagt tcgcgcgcga
     3241 tctcgcggtt ctcccccacg agcgagtcga tgtcgggcga gtcgacgagt accaggccgc
     3301 gtggcacggc gtcggacgcg cggctcgtgg cgcgccgcgt ggccgagctg agcaggaccc
     3361 cgtccgcggg gtggtggaag aggtgcggca ccttggtcgt cggcctaagg acgccagccg
     3421 cggtgacgtc gtcgcccagc agcgcgttga cgatcgtgga cttccccgat ccggtcgaac
     3481 ccgccacgac gaccacggcc ggcacggatc cctcggtcac gcgcgggatg aggtgctggt
     3541 cgagttgctc gaggatgtcg tggacgagcg ccttcgcgcg ctccgcctcg ggcgactcga
     3601 acggcagatc cacgctcccg acggccgagc gcgtggcccg gagtgcatcg agcaacgctc
     3661 ccgggcggcc ccaggaccgg acgggacgga tagtcatggc actagcgtgc ccctttggga
     3721 cccgttcgcc aaacgtccgc gccgtaggcg cctctgggct ccgaactact accctggaag
     3781 gcgcacgccc cgcccccgta gctcagctgg atagagcgcc ggacttctaa tccgtaggtc
     3841 gcaggttcga atcctgccgg gggcaccata gtggtgaggt cgaagcgcga cgggatggcg
     3901 ctccgcgctc ctgacctagg acgcgtccgc cttggggcgt cggcgatccc ggcgacctcc
     3961 gcgacccctg ctcccccacg cggccacgga gcccgtgtgc gggccttcaa cctcttcgag
     4021 gccgaacgcc cggacgagtt ccgcccgggt ctccggtccc gcgaagatca ccacgtcgtc
     4081 ccccgctcgc aacgtggtcg aaggcctcac cggcacgagt tggccagcgc gcacgaccat
     4141 gcttacccac gcctcgtcgg agacctcggt gaggtcctgg atgcttcggc cgtcggcggg
     4201 cgagcccttc acgaccgtaa gccggtgaac cccgtccggc tcctcccgga gccgaacgcc
     4261 gagggcccac ggctgcggtt cgatgacgcg cacgggaatg cgtagccatc gggcgacggc
     4321 tggggtgagg ctcccctgca ccacgacgga gaacaccacg accaccacca cgatcccgtg
     4381 gaggcggtgc gcatcgggca cgtgcgcgac caggaggaag ctcccgagca ggatgggaac
     4441 ggcccccttg aggccagcga agaggacgaa cgccgattgg ctccggggaa gcctgctcgg
     4501 gatgagacac aggcccacca gaaccgggcg aatgaccagc gccacgacga ccgcgagaat
     4561 caggccggga atccagacgt cgccgcggga gacgaccccg aggtcgacgg tgagtccgag
     4621 ggcgaggaac gcgacgatct cgccgaggct cgcgagcgcg ccatggaagc gctcgatctc
     4681 ccgcttgaac ggcgccggtt cgtcgccgat gacgatcccg gcga
//
LOCUS       DASVKP010000208         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1305801, whole genome shotgun sequence.
ACCESSION   DASVKP010000208 DASVKP010000000
VERSION     DASVKP010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305801"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VF362_04145"
     CDS             <1..837
                     /locus_tag="VF362_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX7590151.1"
                     /translation="CCIVFLAPSYLAGAAKNRRWRLLPLTFIFTAGLAVVLVPLTMGA
                     SLVAGAVAKYHAPLYVAGGLLMFAMAYLTLSGKMWSLPGVLRMPDTSRGDSASFFALG
                     VFSGIASSCCAPVLVGVMTLSALAGNAAGGAVLGLAYVFGMVFPLFAMALVWDKTRLS
                     RVRLGRSRPIVITVRGRTLRTTLLNVVIASSFIAMGAAIIALSQSRAMTGGSAIQDWI
                     SLHLVLVFSKVEEWLRPVPEFILGLALFAVVAGIVLATISGRPRPPARPSAQSHERID
                     AP"
     gene            954..1463
                     /locus_tag="VF362_04150"
     CDS             954..1463
                     /locus_tag="VF362_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEX7590152.1"
                     /translation="MGGLIVVVGLVTAGLLSARPQVSEASGLAPDFTLPDTDGNMITL
                     SSLRGHPVLLYFSEGAGCEACIYQMKAIEGDPAFAAAGIVVLPIVMDPPDQIRAAMTY
                     DGVATPFLMDDGKVSAAYKTLGTGMHAGLPGHGFVLINAAGDQVWSGNYPSMWLPPAD
                     LLAEVQTKL"
BASE COUNT          232 a          608 c          506 g          295 t
ORIGIN      
        1 tgctgcatcg tgttcctggc cccgagctac ctcgcgggcg ccgcgaagaa ccgccgctgg
       61 cgcctcctgc ccttgacctt catcttcacc gcgggtctag cggtggtcct cgtcccgctc
      121 accatggggg ccagcctcgt cgcgggggcc gtcgccaagt accacgcccc cctctacgtc
      181 gccggcggcc tgttgatgtt cgcgatggcg tatctcacgc tcagcgggaa gatgtggtcg
      241 cttcccggcg tcctgcgaat gccggacacg tcgcggggtg actccgcctc gttcttcgcg
      301 ctcggcgtgt tctccggaat cgcttcgagt tgttgcgcgc cggtgctcgt cggcgtcatg
      361 accctctcgg ccctcgccgg gaacgcggcc ggcggcgccg tactcggcct cgcctacgtc
      421 ttcggcatgg tcttccccct cttcgcgatg gcgctcgtgt gggacaagac ccgactctcg
      481 cgcgtgcggc tcggacggtc tcggccgatc gtcattacgg tccgcgggcg cacgctacgg
      541 acgaccctgc tcaacgtcgt gatcgcgtcc tcgttcatcg ccatgggcgc ggcaatcatc
      601 gcgttgtccc agagccgggc catgaccggg ggatcggcca ttcaggactg gatcagcctc
      661 cacctcgtgc tcgtcttctc gaaggtcgaa gagtggctcc gtcccgttcc ggagttcatc
      721 ctcggcctcg ccctcttcgc cgtggtcgcg ggaatcgtcc ttgccacgat ttcagggcgc
      781 ccgcgtcccc cggcccgccc gtctgcccaa tcacacgaaa ggatcgacgc gccatgaccc
      841 gtcattcaga ctccgaagca aagtcacggg caccccgtgg gtccggggct cccgggccct
      901 acaacgcgac cgtcgcactg cgccgaactc ggctccgcac cacactcgcc tgggtgggag
      961 gactcattgt cgtcgtcggc ctcgtcactg cgggactgct ctcggcacgc ccgcaggtct
     1021 cggaggcgtc ggggctagcg ccggacttca ccctccccga caccgacggg aacatgatca
     1081 ccctctccag ccttcgcgga catccggtcc tcctctactt cagcgagggc gcggggtgcg
     1141 aggcgtgcat ctaccagatg aaggccattg agggcgaccc cgccttcgcc gcggcgggaa
     1201 tcgtcgtgtt gcccatcgtc atggacccgc ccgaccagat ccgcgcggcg atgacatacg
     1261 acggcgtcgc gacacccttc ctgatggacg acggcaaggt ctccgccgcg tacaaaaccc
     1321 tgggaacggg aatgcatgcc gggcttccgg gccacggctt cgtcttgatc aacgccgcag
     1381 gcgaccaggt ctggtcggga aactacccct cgatgtggct gcccccggcc gacctcctcg
     1441 cggaggttca gacgaaactc tgaacctctc gtcgatgccc gctgccccag cgggccaagg
     1501 cgtgccgcgc cctgccaggc gcggcacgcc tcatccccgg gcccgagaca ccgacagccc
     1561 cgtcatgatg acggcgtggg ccaagcagat gacgcacgtg ccacctcgga tggcgtcctg
     1621 acgttcctgt aggaagtcag c
//
LOCUS       DASVKP010000209         3842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049 k141_64077,
            whole genome shotgun sequence.
ACCESSION   DASVKP010000209 DASVKP010000000
VERSION     DASVKP010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64077"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VF362_04155"
     CDS             <1..270
                     /locus_tag="VF362_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012299309.1"
                     /note="catalyzes the formation of D-glucono-1,5-lactone
                     6-phosphate from D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX7590153.1"
                     /translation="RDVTMDFGYGSSFNETSPEAYERLILDVLLGDPPLFPRFEEVAL
                     SWGILDPIEKYWAEGGRPESYRSGTWGPEAADAMMARDGRAWRRP"
     gene            267..1181
                     /locus_tag="VF362_04160"
     CDS             267..1181
                     /locus_tag="VF362_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX7590154.1"
                     /translation="MILALPNTTTSDINAELVRIRAEGGVVTLGRVMTLVIDATCCDA
                     ESAINAANYASREHPCRVVVIARSAATEPRLDAEIRVGGDAGASEVIVLRPSGDMTQH
                     GDTLLIPLLLPDAPIVAWWPGGTVSAPALTAIGAMATRRITDVKGSPDVKAALLALAD
                     GYAPGDTDLAWARITRWRALLAAALERTGSPTVTHVHIHGDTGSPAVLLLGAWLRGNL
                     GCGFDDTYSDEAKGLVSVRLETPEGEISIVRPDGHDATFSQPGLPDHVIALPQRTLQA
                     CLAEDLRRLDADEVYGETLIALAGALRS"
     gene            1178..1900
                     /gene="pgl"
                     /locus_tag="VF362_04165"
     CDS             1178..1900
                     /gene="pgl"
                     /locus_tag="VF362_04165"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX7590155.1"
                     /translation="MTSTTMVYPDATTVAETTAARLLVAIADALSARAEAHIVLTGGT
                     IGIATLARMAASPLADVIDWRGVHIWWGDERFLPPGDHDRNEVQAQRSLLKRLRLPEE
                     NIHRMASSEGATSPEKVARAYAAELARFGDPAPAFDVLLLGLGPDGHVASLFPGRPEV
                     KVAAVSVVAVKDSPKPPPTRLSMTLPTINGAREVWILTSGAEKARAVADSLAHKAGIP
                     GGMVHGTERTLGLIDKAAAASI"
     gene            complement(1926..2276)
                     /locus_tag="VF362_04170"
     CDS             complement(1926..2276)
                     /locus_tag="VF362_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HEX7590156.1"
                     /translation="MAGGNAIRGSRVGAGPMGEAERGESAPRVQIAYFCANDHVVKPS
                     FATTERDEIPLEWDCPRCGGPAGLDRLNPPKPARSEPFKTHLAYVKERRTDAEGEALL
                     SEALEALRARRGGA"
     gene            complement(2286..2537)
                     /gene="secG"
                     /locus_tag="VF362_04175"
     CDS             complement(2286..2537)
                     /gene="secG"
                     /locus_tag="VF362_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX7590157.1"
                     /translation="MHVLVVTLWSVLVVTSVAIIGLVLLHQGRGGGISDMFGGGLASG
                     IQSSGSGEKNLGRITWGVVIVWGIVIVLLGLVTKYNVGA"
     gene            complement(2596..3384)
                     /gene="tpiA"
                     /locus_tag="VF362_04180"
     CDS             complement(2596..3384)
                     /gene="tpiA"
                     /locus_tag="VF362_04180"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX7590158.1"
                     /translation="MTRTPLIAGNWKLNLDHLEATHAVQKLAWLLRDAKHDFSEVEVA
                     VLPAFTSIRSVQTLVDADQLSIRYGGQDLSAHTEGAYTGEIAGSMLAKLGATYVAVGH
                     SERRQFHAEDDSVVNAKVKAAFAAGLVPILCIGEGLDIRKAGDQVPYTLAQLDGALDG
                     VPAKEASTIVIAYEPVWAIGTGEVATPADAQEVCAAIRSRLAGLYSTEIADGVRVLYG
                     GSVKSGNIAAIMAEADVDGALVGGASLDPEEFSKIVRYRSHVVG"
     gene            complement(3388..>3842)
                     /gene="pgk"
                     /locus_tag="VF362_04185"
     CDS             complement(3388..>3842)
                     /gene="pgk"
                     /locus_tag="VF362_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX7590159.1"
                     /translation="DTVKGYLKTAAAKGVDILLPTDVVIADKFAADAATKIVAADAIP
                     DGWRGLDIGPDTSKAFAARILSAKTVVWNGPPGVSEFPAFAAGTRAVAQAMIDSSAFT
                     VVGGGDSAAAVRLLGFDESKFSHISTGGGASLELLEGKVLPGLAVLEG"
BASE COUNT          597 a         1378 c         1285 g          582 t
ORIGIN      
        1 cgtgacgtca cgatggactt cggctacggg agttccttca acgagacgtc tcccgaggcg
       61 tacgagcgcc tgatcctcga cgtcctactc ggcgaccccc cgctcttccc tcgcttcgag
      121 gaggtcgccc tctcgtgggg aattctcgac cccatcgaga agtactgggc cgagggcgga
      181 cggccggagt cgtaccggtc gggtacctgg ggaccggagg cggccgacgc gatgatggcg
      241 cgcgacggcc gggcatggag gcgcccatga tcctcgccct gcccaacacg accacgagcg
      301 acatcaacgc cgagttggtg cgcatccgcg ccgagggagg ggtcgtcacg ctcgggcgcg
      361 tcatgaccct cgtcatcgac gcgacatgct gcgatgccga atcggcgatc aacgccgcga
      421 actacgcctc ccgggagcac ccatgcaggg tggtcgtgat cgctcgttcc gcggcgacgg
      481 agccccgcct cgacgcggag atccgcgtcg gcggcgacgc gggagcctcc gaggtcatcg
      541 tgctgcggcc ctctggcgac atgacgcagc acggcgacac cctcctcatc ccgctcctgc
      601 ttcccgacgc ccccatcgtc gcgtggtggc cgggggggac ggtctcggct ccggctctta
      661 cggcgatcgg tgcgatggcg acgaggcgca tcaccgacgt caagggcagc cccgacgtca
      721 aggccgccct cctcgcgctc gccgacggat acgcgccagg cgacaccgac ctcgcctggg
      781 ccaggatcac gcgctggcgc gctctgctcg ccgccgcgct cgagcggacg ggctcgccca
      841 cggtgaccca cgtgcacatc cacggcgaca cgggtagccc cgccgtcctg ttgctcggcg
      901 cgtggttgcg aggcaatctc gggtgcggct tcgacgacac gtactcggat gaggccaagg
      961 gcctcgtgtc ggtgcggctc gagacccccg agggtgagat ctcgatcgtg cgcccggatg
     1021 ggcacgacgc gacgttcagc cagccgggcc tgcccgatca tgtcatcgcg ctcccccaac
     1081 gaaccctgca ggcctgcctc gccgaggacc tacgcaggct ggatgccgac gaggtctacg
     1141 gcgagacgct cattgccctc gcgggggcgc tccgatcatg acgagcacca cgatggtcta
     1201 ccctgacgcg accaccgtcg ccgagacgac ggcggcgcgc ctcctcgtcg cgatcgccga
     1261 cgccctctct gcccgcgcag aggcgcacat cgtcctcacc ggaggaacga tcggcatagc
     1321 cacccttgcg cgcatggcgg cctcgcccct ggccgacgtg atcgactgga ggggcgtgca
     1381 catctggtgg ggcgacgaac gcttccttcc ccccggcgat cacgatcgca acgaggtcca
     1441 ggcgcagcgc tcgctcctca agcggcttcg cctgccggag gagaacatcc accgcatggc
     1501 atcctcggag ggcgccacaa gcccggagaa ggtcgctcgg gcctacgcgg cggaactcgc
     1561 gcgcttcggc gacccggccc cggcgttcga cgtgcttctc ctcggtctcg ggcccgatgg
     1621 gcatgtggcc tcgctcttcc ccgggcgacc ggaggtgaag gtcgcggcgg tgtcggtcgt
     1681 cgccgtcaag gactccccca agccgccgcc cacgcggctg tcgatgaccc ttccgaccat
     1741 caacggggcc agagaggtgt ggatcctcac gtcgggcgcg gagaaggcgc gcgccgtcgc
     1801 ggactcgctc gcccacaagg cagggatccc cggcggcatg gtgcacggaa ccgagcgcac
     1861 gctcgggctc atcgacaagg ccgcggccgc atcgatctag cccgaccgcc aggtcctacc
     1921 ccggctcagg cgccgcccct gcgggccctg agggcctcga gagcctcgct cagaagcgcc
     1981 tcgccctcgg cgtccgtgcg gcgctccttc acgtaggcca ggtgcgtctt gaagggctcg
     2041 gagcgagcgg gcttgggggg gttgaggcgg tcgagcccgg ccggaccccc gcagcgaggg
     2101 caatcccact cgagcggaat ctcatcccgc tcggtggtcg cgaaactcgg cttgacgacg
     2161 tggtcgttcg cgcagaagta ggcgatttgg acgcgcggtg ccgactcgcc gcgctccgcc
     2221 tcgcccatcg ggccagcccc gacgcgcgac ccgcgaattg cgttgccacc tgccatggct
     2281 gactcctatg cgccgacgtt gtacttggtg acgagcccga ggaggacgat cacaatgccc
     2341 cagacgatga ccacgcccca cgtgatccgc ccgaggttct tctcccccga acccgaggac
     2401 tggatgccgg aggcgaggcc gcccccgaac atgtcggaga tgcctccgcc gcgcccctgg
     2461 tgaagcagga cgagaccgat gatggccacg ctcgtgacca ccaacacgct ccacaacgtc
     2521 acgactagga cgtgcacgat actccctcgg gtcgtctgtg gtactcagga tacgtgacgc
     2581 gcgcctcggc gtccgctacc ccaccacgtg cgagcgatat cgcacgatct tggagaactc
     2641 ctcggggtcc aggctcgccc cgccgacgag tgctccgtcg acgtcggcct cggccatgat
     2701 ggctgcgatg ttgcccgact tgaccgatcc cccgtacagc acgcggacgc cgtccgcgat
     2761 ctccgtggag tacaacccgg cgaggcggga acggatcgcc gcgcacacct cctgagcgtc
     2821 cgcgggcgtg gcgacctcgc ccgtgccgat ggcccagacg ggttcgtacg cgatcacgat
     2881 cgtcgaggcc tccttggcgg gaacgccgtc gagcgcgccg tcgagctggg ccagcgtgta
     2941 gggcacctgg tcccccgcct tgcggatgtc cagaccctcg ccgatgcaca ggatggggac
     3001 gagccccgcc gcgaacgccg ccttgacctt ggcgttcacg acggagtcgt cttccgcatg
     3061 aaactgacga cgctcggaat gacccaccgc gacgtaggtc gcgccgagct tcgcgagcat
     3121 tgacccggcg atctcgccgg tgtacgcccc ttcggtgtgt gcggacaggt cctggccgcc
     3181 gtagcggatg gacagttggt ccgcgtcgac gagcgtctgg accgagcgga tcgaggtgaa
     3241 cgcgggcagg accgctacct cgacctcgga gaagtcgtgc ttggcgtccc tcaggagcca
     3301 ggccagcttc tgcacggcgt gcgtggcctc aaggtgatcg aggttgagct tccagttacc
     3361 cgcaatcagg ggcgtacgcg tcatgtccta gccttccagt acggccaggc ccgggaggac
     3421 cttgccttcg agcaactcga ggcttgcgcc accgccggtg gagatgtgac tgaacttgga
     3481 ctcgtcgaac ccgaggaggc ggaccgcagc ggcggagtcg ccacctccga ccaccgtgaa
     3541 cgcgctcgag tcaatcatgg cctgcgcgac ggcacgcgtg ccagccgcga aggccgggaa
     3601 ctcggagact ccggggggac cgttccaaac cacggtcttc gccgagagga tcctcgcggc
     3661 gaatgccttc gaggtgtcgg ggccgatgtc gagcccccgc caaccgtcgg ggatcgcatc
     3721 cgcggcgacg atcttggttg ccgcgtccgc cgcgaacttg tcggcgatga cgacgtccgt
     3781 cgggagcagg atgtcgacgc ccttggccgc agccgtcttg aggtagccct tcaccgtgtc
     3841 ga
//
LOCUS       DASVKP010000210         6182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_144851, whole genome shotgun sequence.
ACCESSION   DASVKP010000210 DASVKP010000000
VERSION     DASVKP010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144851"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VF362_04190"
     CDS             <1..463
                     /locus_tag="VF362_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7590160.1"
                     /translation="GMHQINCLTSPQWALVSVLIANIWIGIPFNLVLMYSGLQSIPGE
                     VYEAAALDGASRSQVFWRITFPLLRPVSAITILLGLVYTLKVFDIIWIMTRGGPGTTA
                     STTFAIWSYRLGFGGESPDLSPAAAVANLLILVAFAFGLVYIRMQRRLVEQ"
     gene            460..1278
                     /locus_tag="VF362_04195"
     CDS             460..1278
                     /locus_tag="VF362_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7590161.1"
                     /translation="MRKFRAGATTFIGVILTAIMLFPLYWMVNVSLTQPNMLRKDPPS
                     LFPLDPNFEGYRVVLSQQGPYLLTSLIVGLGTVALTVALSAPAAYALAKLRPRGGGTV
                     SFIFLISQMIPTIIMAMGFYLIFNSWGILNTFWGLILADSTLAVPFGVLIFTAFMSGI
                     PDELLAAAHVDGAGPWRTFRSIVMPVSRNSVVTVALFTFLWAWSDFMYASTLDRGGSL
                     QPVTMGIYRYIGNNTQDWNAIMATAVVASIPATFLLVIAQRYVAAGVTAGALKE"
     gene            1342..3021
                     /locus_tag="VF362_04200"
     CDS             1342..3021
                     /locus_tag="VF362_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein"
                     /protein_id="HEX7590162.1"
                     /translation="MSEIPFSTRGSWLDLSPVVGLHRYADDVHLVSHQNGMHAVLALV
                     PEAAGARIATTLAAAPDVLRWNGASGMIEATFDGTDALRLRGDGLSLRIADASGSLTP
                     FTGTYLFTEPGTSCAVFTSYETGRRYRVTPIRGDFSVEGDGQLGTVAREVRVAGRQWE
                     IAIEGFETARHPYDASRTFERLVESNSRAFRDFLDAIAPWRSDESPGAALAAYVLWSA
                     TVSPMGYLGRESILMSKHWMDKVWSWDHAFNALALAPALLPEALDQFLTPFDHQDASG
                     ALPDSVTHSEVLYNYVKPPIHGWALQRLRARANEALTHDQLIEIYRGLSLWTAFWLNY
                     RRVDGHALPYYQHGNDSGWDNATTFDRSRVIESPDLAAFLVVQLDTLIGLARELETGD
                     APGWADERERILAALCSELWDGDRFFARAADSGAPSTSTSLLNLLPIIAAERLPLEMR
                     DRLAAHIRDHLTEFGPATEIPGTEHYEPDGYWRGPIWAPSTVLIESGLRASGHVELAD
                     DVSRRFRTLCERSGFAENFDAVTGAGLRDRAYSWTASAYLILASEAHSRLD"
     gene            3085..3681
                     /gene="recR"
                     /locus_tag="VF362_04205"
     CDS             3085..3681
                     /gene="recR"
                     /locus_tag="VF362_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX7590163.1"
                     /translation="MYDGAVQDLIDELGRLPGIGPKSAQRIAFYLLGADAADVNRLAQ
                     TLLTVKEKVRFCEICGNVAEGERCRICLDPRRTDDIICVVEEPKDVMAVERTREFRGR
                     YHVLGGAIDPMSGVGPDDLRIRELLARLGQETIAEIIIATDPNIEGEATATYLTRLLA
                     PLGVTVSRLASGLPVGGDLEYADEVTLGRAFEGRRVVS"
     gene            3769..5040
                     /locus_tag="VF362_04210"
     CDS             3769..5040
                     /locus_tag="VF362_04210"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX7590164.1"
                     /translation="MSLIVQKYGGSSVSDAAAIKRVAARVAQTKKAGHDVVVAVSAMG
                     DTTDELIELAHAVSAGSHAREMDMLLTSGERISMALLAMAIRDQGVGAQSFTGSQAGV
                     ITTEQHGRARIIDVTPGRVVKAIEAGDVAIVAGFQGVSVDTKNITTLGRGGSDTTAVA
                     LAAALGADVCEIYTDVDGVFTADPRIVPGARRLDRITYDEMLELAASGAKVLMPRCVE
                     YARRYNVPIHVRSSFSGREGTWVVAATEQGDTMEEPIIAGVAHDRSEAKITVIGIPDV
                     PGKAARLFEAVAQADVNVDMIVQNVSAHSTGVTDISFTLPKSDGERAIAAIDGAKADI
                     GHAEVRYDDEIGKVSLIGAGMRSSTGISAKFFGALRDAGVNIEMISTSDIRISVITRA
                     AQLEDAVRAIHTTFGLDSTDGEAVVYGGSGR"
     gene            5037..6092
                     /locus_tag="VF362_04215"
     CDS             5037..6092
                     /locus_tag="VF362_04215"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX7590165.1"
                     /translation="MNAADSGLVVAVVGATGQVGAVMRKLLWERGFQGATVRYFASAR
                     SAGSTLPWGDREIVVEDVATASLAGIDLALFSAGGATSKQYAPLFAAEGAIVIDNSSA
                     WRMDPDVPLVVSEVNPDALDSIPKGIVANPNCTTMAAMPALKVLHDEAGLRRLVISSY
                     QAVSGAGLAGVAELDGQIKAVIDGAVALTHDGAAVAFPAPTAFAAPIAFNVLPLAGTI
                     VDDGLNETGEEKKLRDESRKILGIPGLLVSGTCVRVPVFTGHSLSINAEFDRPISPAR
                     AYELLAGAPGVRVVEVPTPLQVAGGDDSLVGRIRVDEGVSGGRGLAFFVSGDNLRKGA
                     ALNAVQIAQLLVSHIKK"
BASE COUNT          970 a         2220 c         1953 g         1039 t
ORIGIN      
        1 ggggatgcac cagatcaact gtctcacgtc gccccagtgg gcgttggtgt cggtcctcat
       61 tgcgaacatc tggatcggga tcccgttcaa ccttgtgctg atgtacagcg gcctccagag
      121 cattcctggc gaggtctacg aggcggccgc gctcgacgga gcctccaggt cgcaggtctt
      181 ctggcgcatc actttcccgc tcctgcgccc cgtctcggcc atcacgattc ttctcgggct
      241 cgtctacacc ctgaaggtgt tcgacatcat ctggatcatg acgcgcggcg ggccgggaac
      301 gaccgcatcg acgaccttcg ccatctggtc gtatcgcctc ggattcggcg gagagtcgcc
      361 cgaccttagc cctgctgcgg cggtcgcgaa tcttctgatc ctcgtggcgt tcgccttcgg
      421 ccttgtctac attcgcatgc agcgacgatt ggtggaacag tgaggaaatt tcgcgccggc
      481 gcgacgacgt tcatcggcgt catccttacg gcaatcatgt tgttcccgct gtactggatg
      541 gtgaacgtgt cgctgaccca gcccaacatg ctccggaagg accccccgag cctgtttccg
      601 ctcgatccca acttcgaggg ctatcgcgtc gttctcagcc agcagggccc ctacctgctc
      661 acgagcctca tcgtcgggtt gggcaccgtc gccctcaccg tcgcactgtc tgctcccgcg
      721 gcatacgcgc tggcgaaact ccgcccgcgg ggcggaggga ccgtcagctt catcttcctg
      781 atctcacaga tgattcccac gatcatcatg gcgatgggct tctacctgat cttcaacagt
      841 tggggcattc tcaacacttt ctggggcctc atcctcgccg actccacact cgctgttccc
      901 ttcggggtcc tgatcttcac cgccttcatg tccggaatcc ccgacgaact gctcgccgcc
      961 gcgcacgtcg acggcgccgg accatggcgc accttccgct ccatcgtcat gcccgtcagc
     1021 cgcaattccg tggtgacggt ggcgctcttc acgttcctgt gggcctggtc cgacttcatg
     1081 tacgcgtcca ccctcgatcg gggaggctct ctccagcccg tcacgatggg catctaccga
     1141 tacatcggca acaacaccca ggactggaac gccatcatgg cgaccgccgt ggtcgcctcc
     1201 attcccgcga ccttcctgct ggtgatcgcg cagcggtacg tcgcggcagg cgtcaccgca
     1261 ggcgcgctca aggagtgaaa ccctttccgc agattccgag agaccaccat gtcacctgac
     1321 gttgattcac cggcgttcgc cgtgtccgaa atccccttca gcacgcgagg ctcgtggctc
     1381 gacctgtcgc ccgtggtggg cctccatcgc tacgcggatg acgtccacct cgtctcgcac
     1441 cagaacggga tgcacgcggt ccttgcgctc gttccagagg ccgctggcgc gcgcatcgcg
     1501 acgaccctgg cggcggcgcc cgatgtgctg cggtggaacg gtgcctctgg catgatcgag
     1561 gccacgttcg acggcacgga tgccctgcga ctccgtggcg atggcctgtc gctccggatc
     1621 gcggacgcgt cgggaagcct gacgcccttc acggggacct atctcttcac ggagcccgga
     1681 acctcctgcg cggtcttcac ctcttatgag accggcaggc gataccgggt cacgcccatc
     1741 cggggcgact tctccgtcga gggagacggc cagctgggga ccgtcgcccg ggaggtgagg
     1801 gttgcgggac gccagtggga aatcgccatc gaggggttcg agaccgcgcg gcacccgtac
     1861 gacgcctccc ggaccttcga gcgcctcgtc gaatccaact cccgcgcttt ccgcgacttc
     1921 ctcgacgcga tcgccccgtg gcgctcggac gaaagccccg gggccgcctt ggcggcctac
     1981 gtcctgtggt ccgcgacggt ctcgcccatg ggctacctcg ggcgagagtc catccttatg
     2041 tccaagcact ggatggacaa ggtctggagt tgggaccatg cgttcaacgc gctggcactc
     2101 gcgccagccc ttcttccgga agcgctcgac caattcctga ctccgttcga tcaccaggat
     2161 gcgtcgggtg cgcttcccga ctctgtgact cactccgagg tgctctacaa ctacgtcaag
     2221 ccccccatcc acggctgggc actccagcgg ctacgagcgc gcgcgaacga agccctgacg
     2281 cacgaccaac tcatcgagat ctaccgcggg ctcagcctct ggacggcatt ctggctcaac
     2341 tatcggcgcg tcgacgggca cgcgctcccg tactaccaac acggcaacga cagcggctgg
     2401 gacaacgcga cgacgttcga tcgaagccgc gtcatcgagt ctcccgactt ggcggcgttc
     2461 ctcgtggtgc aactcgacac gctcattggg ctcgcgcgtg agctcgagac cggggacgca
     2521 ccggggtggg ccgatgagcg cgagcggatt ctcgccgcgc tgtgctcgga actctgggac
     2581 ggggatcggt tcttcgcccg cgccgcggac tccggcgcac ccagcacgtc aacgagcctg
     2641 ctgaacctcc tcccgatcat cgccgccgaa cgcctcccct tggagatgcg tgatcgactt
     2701 gccgcgcaca ttcgcgatca cctgacggag ttcgggcccg cgacggagat tcccggaacc
     2761 gagcactacg aaccggacgg ctactggcgg gggcccatct gggcgccctc gaccgtcctg
     2821 atcgagtcgg gccttcgggc gagcgggcat gtcgaactcg ccgatgacgt gagcaggcgg
     2881 ttccgtaccc tgtgtgagcg ttccggattc gccgagaact tcgatgcggt gaccggcgcg
     2941 ggcttgcgcg accgcgcgta ttcgtggacg gcgagcgcgt acctcatcct cgccagcgag
     3001 gcccactctc gcctcgacta gcgcaccctc gggcgtcggg cgaggctgca tcgtccgacg
     3061 agcgccgccg cctacgctgg gggcatgtac gacggcgccg ttcaggatct gatcgacgag
     3121 ctcgggcgtc tgcccggaat cgggcccaag agcgcccagc gcatcgcctt ctacctgctt
     3181 ggggccgacg ccgcggacgt caaccgcctc gcgcagacgc ttctcacggt caaggagaag
     3241 gtgcgcttct gcgagatctg cgggaacgtg gccgagggcg agcgctgccg catctgcctc
     3301 gatccgcggc gcacggacga catcatctgc gtcgtcgagg agcccaagga cgtcatggcc
     3361 gtggagcgca cccgcgaatt ccgggggcgc tatcacgtgc tcggcggggc catcgatccc
     3421 atgtcgggcg tcgggcccga cgatcttcgc atccgcgaac tgctcgcgcg gctgggacaa
     3481 gagaccatcg ccgagatcat catcgcgacc gacccgaaca tcgagggcga ggccacggcg
     3541 acctacctga cgcgcctcct cgcgccgctc ggcgtcaccg tctcgcggct cgcgtccggg
     3601 ctcccggtcg gcggcgacct ggaatacgcc gacgaggtga cgctcggtcg ggcgttcgag
     3661 ggacgccggg tcgtcagcta ggcgggacca ggatcccggg ccgctcccgc tagaattggg
     3721 cgagccaccc ggcgcccgcc ggacccgccg cccaacccgg agaccctgat gagcctcatc
     3781 gtccagaagt acggtggttc gtccgtgtcc gacgctgcgg ccatcaagcg tgtggccgcc
     3841 cgggtcgctc agaccaagaa ggctggccac gacgtcgtcg tcgcggtctc cgccatgggc
     3901 gacaccacgg acgaactcat cgaacttgcc cacgccgtct cggcgggctc ccacgcacgc
     3961 gagatggata tgctcctcac ctcgggcgaa cggatctcca tggccctcct cgccatggcc
     4021 atccgcgacc agggcgtcgg ggcgcagtcc ttcaccggat cccaggcagg ggtcatcacc
     4081 accgagcaac acggccgcgc gcgcatcatc gacgtcacgc cggggcgggt cgtcaaggcc
     4141 atcgaggcgg gcgacgtcgc gatcgtcgcc ggcttccagg gcgtctccgt ggacacgaag
     4201 aacatcacca cgctcggccg cggcggctcc gacaccaccg ccgtcgcgct cgcggccgcg
     4261 ctcggcgcgg acgtctgcga gatctacacc gacgtcgacg gcgtgttcac ggcagacccg
     4321 cgcatcgtgc ccggtgcccg tcgcctggat cgcattacct acgacgagat gctcgaactc
     4381 gcggcctcgg gggccaaggt cctcatgccg aggtgcgtcg agtacgcccg ccgctacaac
     4441 gtcccgatcc acgtgcgctc gtccttctcc gggagggaag ggacgtgggt cgtggccgcc
     4501 accgagcagg gagacaccat ggaagagccc atcatcgccg gagtcgcgca cgaccgctcc
     4561 gaggcaaaga tcaccgtcat cggcattccc gacgtgcccg gcaaggccgc gcggctgttc
     4621 gaggcggtcg cgcaggccga cgtcaacgtg gacatgatcg tccagaacgt ctccgcgcac
     4681 tccacgggcg tcaccgacat ctcgttcacg ctgcccaaga gcgacggcga gcgcgccatc
     4741 gccgccatcg acggcgccaa ggcggacatc ggccatgccg aggtgcggta cgacgacgag
     4801 atcggcaagg tctcgctcat cggggcggga atgcgttcca gcacgggcat cagcgccaag
     4861 ttcttcggcg cgcttcgcga cgccggggtg aacatcgaaa tgatctccac ctcggacatc
     4921 cgcatctccg tcatcacccg cgccgcgcaa ctcgaagacg ccgtgcgcgc catccacacc
     4981 acgttcggcc tggactcgac cgacggcgag gccgtcgtct acgggggatc cggacgatga
     5041 acgccgccga ttcgggcctc gtcgttgcgg tcgtcggtgc caccggccag gtgggcgccg
     5101 tcatgcggaa gctcctgtgg gagcgcggct tccagggcgc gacggtccgg tacttcgcct
     5161 cggcccgctc tgcgggttcg acgctgccct ggggagaccg ggagatcgtc gtcgaggacg
     5221 tcgcgaccgc gtccctcgcg ggcatcgacc tggccctctt ctcggccggg ggcgcgacct
     5281 ccaagcagta cgcgcccctc ttcgccgccg agggtgccat cgtcatcgac aactcctcgg
     5341 cctggcgcat ggaccccgac gtcccgctcg tcgtctccga ggtcaacccg gacgccctcg
     5401 actcgatccc caaaggcatc gtcgcgaatc ccaactgcac caccatggcg gccatgcccg
     5461 cgctcaaggt cctccacgac gaggccggcc tccgccgact cgtcatctcc agctaccagg
     5521 ccgtctccgg ggcggggctc gcgggcgtcg cggaactcga cggccagatc aaggccgtca
     5581 tcgacggggc cgtggcgctc actcacgacg gcgcagcggt ggcattccct gcccccacgg
     5641 cgttcgccgc ccccatcgcc ttcaacgtgc tcccgctcgc gggaaccatc gtggacgacg
     5701 ggctcaacga gaccggcgag gagaagaagc tccgcgacga gtcgcggaag atcctcggaa
     5761 tccccggctt gctcgtgagc ggaacgtgcg tccgcgttcc cgtcttcaca ggacactccc
     5821 tctcgatcaa cgccgagttc gaccggccta tctcgcccgc tcgggcctac gagctcctcg
     5881 cgggcgcgcc gggcgtgcgc gttgtggagg ttccgacgcc gcttcaggtc gcgggaggcg
     5941 acgactcgct cgtcggccga atccgcgtcg acgagggcgt ctccggcggc cgggggctcg
     6001 cgttcttcgt ctccggcgac aacctgcgca agggcgccgc gctgaatgcg gttcagatag
     6061 cgcaactgct cgtgagccac atcaagaagt aggggccagt tggtcggggt gtccggcgcc
     6121 ttcggcggct acaaacaccg aacgcagagg cgggacggca acgccgtccc gctatctcag
     6181 tc
//
LOCUS       DASVKP010000211         3336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1138841, whole genome shotgun sequence.
ACCESSION   DASVKP010000211 DASVKP010000000
VERSION     DASVKP010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138841"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VF362_04220"
     CDS             <1..665
                     /locus_tag="VF362_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7590166.1"
                     /translation="QLAVTDSGSGMDAEFLFHVFEPFFTTKESGLGTGLGLATIYGIV
                     KQNDGFASVSSEPGVGTTFCIYLPRVDAAPEPSQEPSPEPPAGKANGRGDATILLVED
                     EPGILALARKALEGQGYVVLPASTGDDALRLAGEHPGTIDLLLTDVIMPEMNGRALAD
                     ELRSTRPNLKVLFMSGYTADVITGHGTPEGDAHFIAKPFHLKDLAEKVREVLGAASSD
                     TP"
     gene            complement(662..1417)
                     /locus_tag="VF362_04225"
     CDS             complement(662..1417)
                     /locus_tag="VF362_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX7590167.1"
                     /translation="MAQMLVVPGAVEDNLGRAVSAIEAAARAHCDVVVLPECLDLGWT
                     FPGARDLAQPVPGPASDRLRDAAASAGVMVVAGLTERDGDRIYNSAVLIVADGTVLAK
                     HRKINELDFARELYSAGTSLQVVQTPLGAIGVTICADNAYPTLALGRALAAMGAKILL
                     SPCAWAVPPGFDNAVRAYGDEWVTAYGALARETGMPVVGVSNVGPVVGGEWDGWACIG
                     ASLAVGSDGEVILQAPFGEAAETLVTVEVPLPG"
     gene            1630..1884
                     /locus_tag="VF362_04230"
     CDS             1630..1884
                     /locus_tag="VF362_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX7590168.1"
                     /translation="MMGWGYWNGGMHAGWWMAMGFFWVAVVVAGVWGLAHLFPRGADS
                     RNPTAILKARLASGEITLAEYKKILAELTPGPSGGSDAGA"
     gene            complement(1856..2971)
                     /locus_tag="VF362_04235"
     CDS             complement(1856..2971)
                     /locus_tag="VF362_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7590169.1"
                     /translation="MSRRPPWRVNLATRLVAGMVIVIAVGGLTALLVAEAVGPSTFDA
                     HLARAGLSPTSAAVVHARAAFSAASGRSLLLGLAAAGVASLAVGIFVARRIGRSLGVF
                     SEAASRVARGEDAALALSLNAGSEFDRLAADFRHMAAQLADADALRRRLTSDVAHELR
                     TPVATLSAYLEGIEDGVRDLDASTLDVLRSATARLARLAADLAEVTSAEAGSSSLRLE
                     RIAVPVLASTAIAAARPGFTAKSVSLELVPSPDVVVQADPERFGQILGNLLDNALRHT
                     PAGGSVSLGWSATDDEVAIVVADTGEGIGPEHIDRVFERFYRADSARDRDRGGSGIGL
                     AVVKGLVQAHGGRVEAASDGPGRGATFTITLPRQIRR"
     gene            complement(2975..>3336)
                     /locus_tag="VF362_04240"
     CDS             complement(2975..>3336)
                     /locus_tag="VF362_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7590170.1"
                     /translation="ARAMLRRPREDASMGAPPAPALRVGALAIDLDAREASVDGVLVD
                     LTKIEFDLLAALALRPRQVFSRRALIAAVWGGEWVGDEHLVDVHVLHVRRKLGDDASA
                     QRFIRTSRGIGYRMGEG"
BASE COUNT          557 a         1189 c         1155 g          435 t
ORIGIN      
        1 tgcaactcgc cgtgaccgac agcggctcgg gcatggacgc cgagttcctc ttccacgtct
       61 tcgagccgtt cttcaccacc aaggagtcgg ggctcggcac ggggctgggt ctcgcgacga
      121 tctacggcat cgtcaagcag aacgatggct tcgcgagcgt gtccagcgag cccggcgtag
      181 gcacgacctt ctgcatctac ctgccccgcg tcgacgcggc gccggagccc tcgcaggaac
      241 cctcgccgga gccccccgca ggcaaggcga acggacgtgg cgacgcgacg atcctgctgg
      301 tggaggacga gcccgggatc ctggccctgg cgaggaaggc gctggagggc cagggctatg
      361 tcgtgctgcc ggcaagcacg ggcgacgacg cgcttcgcct tgccggggag caccccggca
      421 cgatcgacct gctgctaacg gacgtgatca tgcccgagat gaacgggcgg gcgctcgcgg
      481 acgaactgag gtcgacccgc cccaacctga aggtcctctt catgtccggc tacacggccg
      541 atgtcatcac gggtcacggc acgccggagg gcgacgccca cttcatcgcg aagcccttcc
      601 acctgaagga ccttgcggag aaggttcgcg aggtgctggg cgccgcgtcg tcagacacgc
      661 cctagccggg aagggggacc tcgaccgtca cgagggtctc tgcggcctcg ccgaagggcg
      721 cctgcaggat gacctccccg tccgagccga cggcgagcga ggcgccgatg catgcccagc
      781 cgtcccactc gcccccgacg acggggccga cgttgctcac cccgaccacc ggcatcccgg
      841 tctctcgggc gagcgcgccg taggccgtga cccactcgtc gccgtacgcc cgaaccgcgt
      901 tgtcgaagcc agggggcacc gcccacgcgc acggcgaaag cagaatcttg gcccccatcg
      961 cggcgagcgc gcggcccagc gccagggtcg ggtaggcgtt gtccgcgcag atggtgacgc
     1021 cgatggcgcc cagcggcgtc tgaacgacct ggagggacgt cccggccgag tagagttcgc
     1081 gcgcgaagtc caactcattg atcttccggt gcttggcgag gaccgtcccg tcggcgacga
     1141 tcaggacggc gctgttgtag atccggtcgc cgtcgcgctc ggtcaggccc gcgacgacca
     1201 tgaccccggc cgacgccgcg gcgtcgcgca ggcggtccga cgcgggcccg gggacgggct
     1261 gggcgagatc gcgggcgccg gggaaggtcc aaccgaggtc gagacactcc ggcaggacga
     1321 cgacgtcgca gtgggcgcgc gcggccgcct cgatcgccga gaccgcgcgc ccaaggttgt
     1381 cctcgaccgc gcccgggacg accagcatct gcgccatacc gacccgaagc gtgcggtgat
     1441 cgatcggcaa ccgaatcccc tcctgatgag accggaccac gctcccccgg cgatgtctca
     1501 ccccagtcaa ccaccgcgtc ggcccgcgcc ttcatcgaag attcacacag ccccaacctc
     1561 atctccacgc gacgggggtg gaatgaaggg gacgaggcgc tcccgacctc gcggacgaca
     1621 ggagcagtca tgatgggctg gggctactgg aatggcggca tgcacgcggg atggtggatg
     1681 gcaatgggat tcttctgggt cgcggttgtt gtggccgggg tctgggggct cgcgcacctg
     1741 tttccgcgcg gcgccgactc caggaacccg accgcgatcc tcaaggcgcg gctcgcgtcg
     1801 ggcgagatca ccctcgcgga gtacaagaag atcctggcgg aactgacgcc cgggcctagc
     1861 ggcggatctg acgcgggagc gtaatggtga acgtcgcgcc gcggcccggg ccgtcggacg
     1921 cggcctccac cctgccgcca tgtgcctgga cgagtccctt cacgacggcg aggcctatgc
     1981 ccgatccgcc ccggtctcgg tcgcgcgcag aatccgcccg gtagaagcgc tcgaacacgc
     2041 ggtctatgtg ctcgggaccg atgccttctc ccgtatccgc gaccacgatc gccacctcgt
     2101 cgtcggtcgc cgaccacccg agcgacacgc tcccgcccgc aggcgtgtgg cggagagcat
     2161 tgtccaacag gtttcccagg atctggccga accgttctgg gtccgcctgg accacgacgt
     2221 cgggggatgg caccagctcg agcgaaacgg acttcgcggt gaaccccggc cgcgccgccg
     2281 cgatcgcggt cgaggcgagg acgggcactg cgatgcgttc aagccgaagg ctcgacgagc
     2341 ccgcctccgc cgatgtcact tccgcgaggt ccgcggcgag cctcgcgagc cgggctgtcg
     2401 ccgaacggag aacgtcgagc gtcgaggcat caaggtcgcg aacgccgtcc tcgatgccct
     2461 ccaggtatgc ggagagggtc gccacggggg tgcgaagctc gtgggcaacg tccgacgtca
     2521 ggcgtcgccg aagagcatcg gcgtccgcga gttgcgccgc catgtgacgg aagtccgcag
     2581 cgaggcggtc aaactcgctg cccgcgttga gcgacagcgc gagcgccgcg tcctcgcctc
     2641 gcgcgacgcg cgaggccgcc tccgagaaca ccccgagcga tcgcccgatc ctgcgcgcta
     2701 cgaaaatgcc gaccgcgagg gacgccacgc cagcggccgc cagaccaagg aggagcgaac
     2761 gcccgctcgc cgcggagaac gcggcccgcg cgtgcacgac ggcggcggat gtgggactga
     2821 gcccggcgcg ggcgaggtgg gcgtcgaacg tggaaggccc cacggcctcg gcgacgagaa
     2881 gcgcggtgag ccccccaacc gcgatgacaa tcaccattcc cgcgacgagc cgcgtcgcaa
     2941 ggttgacccg ccagggcgga cggcgagaca cgggctaccc ctcccccatg cgatatccga
     3001 tgccccgcga cgttcggatg aagcgttgcg ccgaggcgtc atcgccgagc ttgcgccgga
     3061 cgtgcaaaac atgaacatcc acgagatgct cgtcgccgac ccactcgcct ccccacaccg
     3121 ccgcaatcag ggccctccgg gaaaagacct gccggggtcg caacgcgagc gcggcgagga
     3181 ggtcgaactc gatcttcgtc aaatcgacga ggacgccatc aacgctcgcc tctcgggcgt
     3241 caaggtcaat cgcgagcgcg ccgacgcgga gtgccggcgc gggcggcgcc cccatggacg
     3301 cgtcttcacg gggccgccgc aacatggctc gggcgc
//
LOCUS       DASVKP010000212         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049 k141_64584,
            whole genome shotgun sequence.
ACCESSION   DASVKP010000212 DASVKP010000000
VERSION     DASVKP010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64584"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            381..638
                     /locus_tag="VF362_04245"
     CDS             381..638
                     /locus_tag="VF362_04245"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009883710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX7590171.1"
                     /translation="MTTRPDIEALSYEQARDELVGIVAKLEIGAVTLEESLELWERGE
                     ALAVRCQHILQGARERVAGAGGDKAGDKSGDDASPDAGTSE"
     gene            635..1567
                     /locus_tag="VF362_04250"
     CDS             635..1567
                     /locus_tag="VF362_04250"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEX7590172.1"
                     /translation="MSDHALVIGEALMDVVIRRDGTSTEHPGGSPANVAIGLARLGRQ
                     AELLAWIGLDDRGRLIREHLESAGVTLVRGSESAIHTPVATAVLDDTGAADYDFDLAW
                     AISPNAAPTRKPLVVHTGSLGAILPPGGNDVLTLLEGFRSTATITYDPNVRPSLMGNP
                     ITAAAQIDHVVALADIVKVSDQDLQWLHSDEPINAQVRRWLRMGPSIVVVTRGERGAS
                     AFLKDARRIDVTAAKVEVVDTVGAGDAFMAGLIDGLWSQGCLGAATRDDLDTISTDII
                     TYVLARCVGIATITVSRAGASAPTRAELGETPLS"
BASE COUNT          291 a          627 c          580 g          266 t
ORIGIN      
        1 ccgagggttt ctcgctaccc ccgcccgaac ggcgggcgac tcgtcggccg cgagaatcag
       61 gagggacgtc aggggcgtcc cgacgcgctc accaacggcg gcgcgaacct tgagttggtc
      121 cgaacgcgcc agcgcggcga gttctccgtc ggtcaggtca tccctcatca ctcttgccac
      181 gctgctcagg gcggtccggc caccctcgta gtcatcactg aacatttcga ttcaccccat
      241 tgtgagtcgg ttgattcctt ctatggatga gacgtcccgg cggccgcact atgacgccgc
      301 ttcccgaagt tcgcgaaacc ctctggctcc gagcccgcca accgacgcct cgcggggtgc
      361 tctcgcggta gcgtgggacc gtgaccaccc gacccgatat cgaggccctc tcctacgagc
      421 aggcgcgcga cgaactcgtg ggaatcgtcg cgaaactcga gatcggtgcc gtgacgctcg
      481 aggagtcgct cgaactctgg gagcggggcg aggcactcgc cgtgaggtgc cagcacatcc
      541 tccagggggc gcgtgaaagg gtcgcgggcg cgggcggcga caaggctggc gacaagtccg
      601 gagacgacgc gtcgccggac gcagggacct cggagtgagc gaccacgccc tcgtcattgg
      661 cgaggcgctg atggacgtcg tgattcgccg agacggcacc tccacggagc acccgggcgg
      721 atcaccggcg aacgtcgcga tcgggctcgc ccgcctcggt cgccaggccg aacttctcgc
      781 gtggatcggc ctcgacgacc gcggccgcct gattcgcgag cacctcgaga gcgcgggcgt
      841 cacactggtc cgcggttccg agtcggcgat acacacgccg gtcgcgacgg cggtcctcga
      901 cgacacgggg gccgcggact acgacttcga cctcgcctgg gcgatcagcc ccaacgccgc
      961 accgacgcgc aagcctctcg tcgtccacac gggttccctc ggcgccatcc ttccgcccgg
     1021 cggcaacgac gtcctcaccc tcctggaggg cttccgatcg acggcgacga tcacctacga
     1081 cccgaacgtg aggccgtccc tcatgggcaa ccccataacc gcggcggcac agatcgatca
     1141 cgtcgtcgct ttggcggaca tcgtcaaggt gagcgaccag gatctccagt ggctgcattc
     1201 ggatgagccg atcaacgcac aggtgcgtcg ctggctccgg atgggtccgt cgatcgttgt
     1261 cgtcacccgc ggagagcggg gcgcgagcgc cttcctgaag gacgcccgcc gcatcgacgt
     1321 caccgccgcg aaagtcgagg tggtggacac cgtcggcgcg ggcgacgcgt tcatggcggg
     1381 cctgatcgac gggctgtggt ctcagggctg cctcggcgcg gcaacccgcg acgacctcga
     1441 taccatctcc accgacatca tcacctacgt gctcgcccgg tgcgtgggaa tagcgaccat
     1501 cacggtctcc cgggctggcg caagtgcgcc tacccgcgcc gaactcggcg agacccctct
     1561 gtcgtaggcc agcagcgtcc taggccagca gcgtcctagg ccaggagacg cccctgcgcg
     1621 aggcccctca cggcccgcgg accgacgtcg gcgatacgcg cgaccacggc aaggtgaacg
     1681 gccgagttct cgacgagggc gcgagcgaca tccgcgctct tgtcccgcga aagagtttcc
     1741 gcgattgagg tcgcgcgtcc aatc
//
LOCUS       DASVKP010000213         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_728001, whole genome shotgun sequence.
ACCESSION   DASVKP010000213 DASVKP010000000
VERSION     DASVKP010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728001"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1551)
                     /gene="ilvD"
                     /locus_tag="VF362_04255"
     CDS             complement(<1..1551)
                     /gene="ilvD"
                     /locus_tag="VF362_04255"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX7590173.1"
                     /translation="MSNAPDIKPRSRQVTDGLEATAARGMLRAVGMGDDDWVKPQIGV
                     ASSWNEITPCNLSLDRLAKAAKEGVHAAGGYPLEFGTISVSDGIAMGHEGMHFSLVSR
                     DLIADSVETVMQAERLDGSVLLAGCDKSLPGMLMAAARLDLASVFLYAGTIAPGWVTL
                     EDGTEKTVTIIDAFEAVGACARGLMSVGDVDRIERAICPGEGACGGMYTANTMASAAE
                     AIGMSLPGSAAPPSADRRRDAFAHKSGEAVVNLLRKGITARDIMTKEAFENAIAVVMA
                     FGGSTNAVLHLLAIANEAEVDLSLADFSRIGDKVPHLGDLKPFGAYVMNDVDRVGGVP
                     VVMRALLDAGLIHGDALTVTGMTVAENLADINPPDLDGKILRALDNPIHKTGGITILS
                     GSLAPDGAVVKSAGFDDNVFEGTARVFERERGALDALENGTIKAGDVVVIRYEGPKGG
                     PGMREMLAITAAIKGAGLGRDVLLVTDGRFSGGTTGLCVGHIAPEAVDAGPIAFVRDG
                     DRITLDVAN"
     gene            1650..>2641
                     /locus_tag="VF362_04260"
     CDS             1650..>2641
                     /locus_tag="VF362_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX7590174.1"
                     /translation="MPSALQIYKRVVLGRPIANEHLEGQGLSKRIALPVFASDALSSV
                     AYGPQELLMILALGGLSFLSFAPWVAAAIMGLLMVVVISYRQLIRAYPSGGGSYEVAH
                     KNLGAKAGLVVGAALLVDYIMTVAVSIASGVDNIISAAPEINRFRVEMAVGFVVILVA
                     MNLRGVRESGTAFSIPTYLFIGSVGITIVVGLIRHLMGTLPLAESAGYTVPERSMGQV
                     AVVLLLMRGFASGCATLTGVEAVANGVPAFRPPKSRNAQVTLTLMGIIAVTLMAGIVT
                     LAMMVKVHYVENLCTVQGYVGDCTTDPQRSVIAQIAHAVFGSDHHLGFYAVQATT"
BASE COUNT          385 a          959 c          845 g          452 t
ORIGIN      
        1 gttcgccacg tcgagcgtga tcctgtcgcc gtcgcgcacg aaggcgatcg gcccggcgtc
       61 gacggcctcc ggcgcgatat gcccgacgca cagccccgtc gtcccgccgg agaagcgccc
      121 atcggtcacc agcagcacgt ccctgccgag cccagcgccc ttgatcgcgg ccgtgattgc
      181 gagcatctcg cgcatgcccg gaccgccctt cggtccctcg tagcggatga ccaccacgtc
      241 ccccgccttg atcgtcccgt tctcgagggc gtcgagcgcg ccgcgctcgc gctcgaagac
      301 ccgcgcggtg ccctcgaaca cgttgtcgtc gaagcccgcg gacttgacca cggcgccgtc
      361 cggcgcgagc gagccactca ggatggtgat gccgccggtc ttgtgaatcg ggttgtcgag
      421 cgcacgcagg atcttgccgt cgaggtcggg cgggttgatg tcggcgaggt tctccgcgac
      481 cgtcatgccc gtcaccgtga gcgcgtcgcc gtggatgagc ccggcgtcga gcagggccct
      541 catcaccacc ggcaccccgc cgacgcggtc gacgtcgttc atcacgtacg cgccgaaggg
      601 cttgaggtcc ccgaggtgcg gaaccttgtc gccgatgcgc gagaagtcgg cgagggacag
      661 gtccacctcg gcctcgttcg cgatcgcgag caggtgcagc acggcgttag tcgagccgcc
      721 aaacgccatg acgacggcga tggcgttctc gaacgcctcc ttggtcatga tgtcgcgcgc
      781 cgtgatcccc ttgcgaagga ggttcaccac ggcctcgccg gacttgtgcg cgaaggcgtc
      841 gcggcgtcga tccgcgctcg gcggggcggc cgagcctggc agggacatcc cgatggcctc
      901 ggccgcggat gccatggtgt tcgcggtgta catgccgccg cacgcgccct ctcccgggca
      961 gatggcccgc tcgatccggt ccacgtcccc caccgacatc aggccgcggg cgcacgcgcc
     1021 cacggcctcg aaggcgtcga tgatcgtgac cgtcttctcc gtgccgtcct cgagcgtcac
     1081 ccatccgggc gcgatcgtcc ccgcgtagag gaacaccgac gcgaggtcga gtcgcgccgc
     1141 ggccatgagc atccccggca gggacttgtc gcacccggca agaaggacgg agccgtccag
     1201 gcgctccgcc tgcatcaccg tctcgacgga gtcggcgatc aggtcgcgcg acaccagcga
     1261 gaagtgcatg ccctcgtggc ccatggcgat gccgtcgctc acggagatgg tgccaaactc
     1321 gagcgggtag ccccccgccg catggacgcc ctccttcgcg gctttcgcga ggcggtcgag
     1381 cgagaggttg cagggggtga tctcgttcca cgacgaggcg acgccgatct ggggcttgac
     1441 ccaatcgtca tcccccatac ctaccgcgcg aagcatcccg cgcgccgctg tggcctcgag
     1501 cccgtccgtc acctgacgcg aacggggctt gatatcgggt gcgttgctca cgcgcacgag
     1561 tctaggtctc ctcccgcgcg cagcgccgcc gtgacgctcc tgacgagggt tcacgcggca
     1621 ctcccccggc gggagtagca tcgtcgcttg tgccgtccgc gctccagatc tataagcgag
     1681 tagtcctcgg tcgaccgatc gcgaacgagc accttgaggg ccagggcctc agcaagagga
     1741 ttgcccttcc cgtcttcgcg tcagacgcgc tctcctccgt cgcgtatggc ccgcaagaac
     1801 tcctcatgat ccttgcgctc ggcgggctct ccttcctctc gttcgcgccg tgggttgctg
     1861 cggcgatcat ggggctcctc atggtggtcg tcatctcgta tcgccaactc attcgcgcct
     1921 acccctcggg cggcggctcg tacgaggtcg cccacaagaa cctcggcgcg aaggccggcc
     1981 tcgtcgtcgg ggcggccctc ctcgtcgact acatcatgac ggtcgccgta tccatcgcgt
     2041 ccggcgtgga caacatcatc tccgcggccc ccgagatcaa ccgcttccgc gtcgagatgg
     2101 ccgtcggttt cgtcgtcatc ctcgtcgcca tgaatctccg cggcgtgcgc gagtctggca
     2161 cggccttctc catccccacc tacctcttca tcggcagcgt cggcatcacg atcgtggtcg
     2221 gcctcattcg gcacctcatg gggaccctcc cgctggccga gtccgcaggg tacacggtcc
     2281 ctgagaggtc catggggcag gtggcggtgg tgctccttct gatgcgcggc ttcgcgagcg
     2341 ggtgcgcaac cctaacgggg gtcgaggccg tcgccaacgg cgttcccgcg ttccgtcccc
     2401 cgaagtcccg caacgcccag gtaaccctca ccctcatggg gatcatcgcg gtgaccttga
     2461 tggccgggat cgtcacgctc gcgatgatgg tcaaggtcca ctacgtcgag aacctttgca
     2521 ccgtccaggg atatgtcggc gactgcacga ccgacccgca gcgaagcgtc atcgcgcaga
     2581 tcgcgcacgc cgtcttcggg agcgatcatc acctcgggtt ctacgcggtc caggcgacca
     2641 c
//
LOCUS       DASVKP010000214         4499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1306863, whole genome shotgun sequence.
ACCESSION   DASVKP010000214 DASVKP010000000
VERSION     DASVKP010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306863"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..423
                     /locus_tag="VF362_04265"
     CDS             220..423
                     /locus_tag="VF362_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010533274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7590175.1"
                     /translation="MDNEIRTRREAEGLSQAALAEALGVSRQTVNSLEMGRYDPSLPL
                     AFAIARHFHTTIEEIFHVDHQES"
     gene            401..814
                     /locus_tag="VF362_04270"
     CDS             401..814
                     /locus_tag="VF362_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590176.1"
                     /translation="MLTTKNRRKTLGGAIGGLIGGMAAITAIAWIARARGDDDFAIGV
                     IAGGAAMILFVAIALAFGRRGPSFLRVAGGFADERERILALGAVSHGAFAMVLAACGF
                     AVFWPNGPAIAAAGAVLWSGLLAAAASFLIRALRN"
     gene            874..1617
                     /locus_tag="VF362_04275"
     CDS             874..1617
                     /locus_tag="VF362_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX7590177.1"
                     /translation="MLKRLNEFRRRERPVHGTEQHDRLITRRLNVLRASVLGANDGVV
                     SIAALVVGVAAAAIDSSQIAIAGFAGLAAGALSMAAGEYVSVSSQRDAENAQLARERK
                     WHVERPDWEFEQLVRLNKETGMTEEVARTAAKEQTAHDPLGAHAKLHLGIDDPDFLTS
                     PARAGIASFFAFSMGGTIPILTILLTPASLRIPITFAVAIVCVAGTGWAASQVAHSSK
                     WRAIIRNVIGGIVALAVTYGIGALVGTQV"
     gene            complement(1660..3261)
                     /gene="cimA"
                     /locus_tag="VF362_04280"
     CDS             complement(1660..3261)
                     /gene="cimA"
                     /locus_tag="VF362_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX7590178.1"
                     /translation="MASRDIEVYDTTLRDGAQQEGMNLSVGDKMAIAPLLDELGVDVI
                     EGGWPGAIPKDTEFFALAHKELKFSHATFAAFGATRKPGTPTAQDAQVRALLDSEASV
                     ITLVAKSDIRHVELALKTTGEENLAMIAETIAFLVAHERRVILDAEHFFDGYRFDPLY
                     GVRALKAAADAGASTLVLCDTNGGMLPDDVARIVAEVAPQVSAALGIHAHNDSGCAVA
                     NTMAAVSSGAVHVQGCVNGYGERTGNADLIAVAANLEIKKGLIALKNNGLAEATRIAH
                     AVAEITNIAPFARQPYVGHSSFAHKAGLHASAIRVDPDLYQHTRPELVGNDMRMVVSE
                     MAGRASVELKGKELGFDLAHQNELLGRVTDRIKNAEAQGYTYDAADASFELLLRDEMG
                     DAPRFWETEGWSVLVQTVPGDPANAGAEATVKLRAGGRGATASAEGNGPVNALDHALR
                     FAVVGTYPEIERFDLTDFKVRIVDASHGTDAVTRVLITTTDTQTATEWRTVGVGPNVI
                     EASWEALTEALRYGLIHAGVSPREG"
     gene            3620..>4499
                     /locus_tag="VF362_04285"
     CDS             3620..>4499
                     /locus_tag="VF362_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7590179.1"
                     /translation="MRLQFLQRSGSAAYWALLTFCFLVVFAIDLLLPYGYIAAALYVI
                     PVFLTVLTGRVAWVGRTTIAAVVATLLGFLNTPGSAVAPDASQHAFTRVTIAVMILAA
                     GAAGSLLIVRSNRLTAVSGELSSTSDELEKQAKLIRAASQIGKIAGWSLAREEGSSIV
                     WSEAVEPIFGLEPGARSLPSDALAFVPDSRDRDRLKGAAFDCLRDGTPFVETFRAMST
                     GGRLLWATVMGEATRNDLGRIVGIAGALQDVTLWKEAEATAATQRRRFTQFANSLPSI
                     IWTAEPSGEIDYFNDAV"
BASE COUNT          660 a         1538 c         1533 g          768 t
ORIGIN      
        1 gggagacttc gagtgggcga tagccgaggg cgcgacgatc gtgcgcgtcg gcagcgcggt
       61 cctcggcccg cgggcctggc gcccgggccg gacgccgtag tcggaccccg tagccggaca
      121 gcgcgcaaca tgacaaggtg acttgacaat cgatctcgtg tcaagcatgc ttgtcctgtc
      181 aaggtcgttt gacatcgagg cgaacgagca ggaggcggca tggacaacga gatccggacg
      241 cgacgcgagg cggagggact ttctcaggcc gcactcgccg aagctctcgg ggtgtcccgc
      301 cagacggtca actccctaga gatggggcgg tatgacccgt ccctccccct cgccttcgcg
      361 atcgcgcgac acttccacac caccattgag gagatctttc atgttgacca ccaagaatcg
      421 taggaagacg cttggcgggg cgatcggcgg cctgattggc gggatggccg ccatcaccgc
      481 catcgcttgg attgcgcgcg cgcgcggcga tgacgacttc gcgatcgggg tcattgcggg
      541 gggcgcggcg atgatcctct tcgtcgccat cgccctggcg ttcggtcgcc gagggccatc
      601 gtttctgcgc gttgccggag gcttcgccga cgagcgcgag cgcatcctgg ccctcggcgc
      661 ggtctcccat ggcgccttcg cgatggtgct cgcggcctgt ggattcgcgg tcttctggcc
      721 caacggtccc gccatcgccg ccgccggggc ggtcctctgg tccggcctcc tcgccgccgc
      781 cgcgagcttc ctcatccggg cactgcgcaa ctagcggcga gatgaggcga cccttccatg
      841 acagggtccg cgcgcgccag gtactctagc gccatgctga agcgcctcaa cgaattcagg
      901 cgccgcgagc gccccgtcca cggaacagaa cagcacgacc gactcatcac tcgccggctg
      961 aacgtgctcc gcgcgtccgt cctgggcgcg aacgacggcg tcgtgagcat cgccgccctc
     1021 gtcgtcggcg tcgcggcggc cgctatcgac tcgtctcaga tcgccatcgc gggcttcgct
     1081 ggcctcgccg caggcgccct ctccatggcc gccggggagt acgtgtcggt gagctcgcag
     1141 cgcgacgccg agaacgcgca actggcccgg gaacgcaagt ggcacgtcga gcgccccgat
     1201 tgggagttcg agcaactggt tcgtctcaac aaggagacgg gcatgacgga ggaggtcgcg
     1261 cggaccgcag cgaaggagca gacggcgcac gacccgctcg gcgctcacgc caaactgcac
     1321 ctgggaatcg acgaccccga cttcctcacc agccccgcga gggcgggcat cgcctccttc
     1381 ttcgcgttct ccatgggcgg cacaattccc atcctgacga tcctcctgac ccccgcatcg
     1441 ctgcggattc cgatcacgtt cgccgtcgcc atcgtctgcg tcgccgggac ggggtgggcg
     1501 gcctcacagg tggcgcactc gtcgaagtgg cgcgccatca tccgcaacgt gatcggtggg
     1561 atcgtcgcgc tcgcggtgac ctacgggatc ggcgcgctgg tcggtacgca ggtctagccg
     1621 cgccggtccg gacgggcacc agcccagaag ggcgccggtc tagccctccc gcggcgaaac
     1681 gcccgcgtga atgagcccgt agcgcagagc ctcggtgagc gcctcccatg aggcctcgat
     1741 gacgttcggc cccacgccga cggtccgcca ctcggtcgcg gtctgcgtgt ccgtcgtcgt
     1801 gatgaggacg cgcgtcacgg cgtccgtgcc gtgactcgcg tcgacgatgc gcaccttgaa
     1861 gtcggtcaga tcgaaccgct cgatctcggg gtaggtcccg acgacggcga accgcagggc
     1921 atggtcgagt gcgttgaccg ggccgtttcc ctccgcgctc gcggtggcgc ccctgccgcc
     1981 cgcgcgcagt ttcaccgtgg cctccgcccc ggcattggcg ggatcccccg gaacggtttg
     2041 aaccaggacg ctccatccct ccgtctccca gaagcggggc gcgtcgccca tctcgtcgcg
     2101 gagaaggagc tcgaacgacg cgtcggccgc gtcgtacgtg tagccctggg cctcggcgtt
     2161 cttgatgcgg tcggtcacgc ggccgaggag ttcgttctgg tgggccaggt cgaacccgag
     2221 ttccttgccc ttgagctcca ccgacgcgcg gccggccatc tccgagacga ccatgcgcat
     2281 gtcgtttccg acgagttccg gtcgcgtgtg ctggtagagg tcggggtcga cgcggatggc
     2341 ggacgcgtgg aggcccgcct tgtgagcgaa cgagctatgc cccacatagg gctgtcgcgc
     2401 gaagggggcg atgttcgtga tttcggcgac ggcgtgcgcg atgcgggtcg cctcggcgag
     2461 cccgttgttc ttcagcgcga tgagcccctt cttgatctcg aggttcgccg ccacggcgat
     2521 gaggtccgcg ttgcccgtgc gctctccata gccgttgacg catccctgga cgtggacggc
     2581 acccgaggag acggcggcca tggtattcgc caccgcgcaa ccggagtcgt tgtgcgcgtg
     2641 gatgccgagc gccgccgaga cctgcggcgc gacctcggcg acgatgcgcg ccacgtcgtc
     2701 gggcagcatg ccgccgttcg tgtcgcacag cacgagggtg ctcgccccgg cgtcggccgc
     2761 cgccttgagc gcccgcacgc catacagcgg atcgaaccgg tagccgtcga agaagtgctc
     2821 ggcgtccagg atcacgcggc gctcgtgggc gacgaggaag gcgatggtct ccgcgatcat
     2881 cgcgaggttc tcctcgccgg tcgtcttgag ggcgagctcc acatggcgga tgtcggactt
     2941 ggccacgagc gtgatgaccg aggcctccga gtccaggagc gcgcgcacct gagcgtcctg
     3001 ggccgtgggg gtaccgggct tgcgcgtcgc gccgaaggcg gcgaaggtgg cgtgggagaa
     3061 cttgagttcc ttgtgggcaa gggcgaagaa ctcggtgtcc ttggggatgg cgccgggcca
     3121 gccaccctcg atgacgtcaa ccccgagctc atcgagcagg ggcgcgatcg ccatcttgtc
     3181 gcccaccgag aggttcatgc cctcctgctg ggcgccgtcg cgcagcgtcg tgtcgtacac
     3241 ctcgatgtct cgactcgcca tttcgtccct ctttcctcgc gtctcgcgtc tcgcgtcccg
     3301 cacccggccg gcggggccca ataaaaaagc ccccccaaag gtgcgggagg ctgcgcgtcg
     3361 acgtggtgtc gacgcgctac tgaataatcg ctgcgctgtt cattggcacc attgtggcac
     3421 acggcgccgt ttgggtgctt cgcccctttg tcgaaagggt cgcacgccct gggggagcga
     3481 aggcgcgtgc ctagactttg aggcggaggg gtgtgtaccc gcaggcgggg aggctcatgg
     3541 tgcgtgacgg tcggttgaga gtcacggtgc ttgttgcgcg cccgaccaag gcacgcgccg
     3601 ggcgccggga agcgctctcg tgagactgca attcctgcaa cggagtgggt cggcggccta
     3661 ttgggcgctc ctcaccttct gcttcctcgt cgtcttcgcg atcgacctcc tgctccccta
     3721 cgggtacatt gccgccgcgc tctacgtcat tcccgtgttt ctcaccgtcc tgacgggccg
     3781 cgtggcgtgg gtcgggcgga ccaccatcgc ggccgtcgtc gcaacgctcc tcggattcct
     3841 caacaccccg ggctccgcag tcgcccccga cgcgagccag cacgccttca cccgcgtcac
     3901 catcgccgtc atgatcctcg cggccggagc agcgggttcg ctcctcatcg tccgctccaa
     3961 ccgtctcacc gcggtctcgg gcgaactgtc atcgacgagc gatgaactcg agaagcaggc
     4021 gaaactgatt cgcgcggcga gtcagatcgg gaagatcgcc ggatggtccc ttgcccgcga
     4081 ggaagggtcg tcgatcgtgt ggtcggaggc cgtcgagccc atcttcggat tggagcccgg
     4141 cgcgcggtct ctaccgagcg atgcgctcgc cttcgtccca gacagccgcg accgggatcg
     4201 gctgaagggc gccgccttcg actgcctgcg agatgggacg cccttcgtgg agaccttccg
     4261 ggcaatgagt accggggggc gcctcctgtg ggcgacggtg atgggcgagg caacgcgcaa
     4321 cgacctcggc cgcatcgtcg gcattgcggg cgcgctccag gacgtcacgc tgtggaagga
     4381 ggccgaggcc acggcggcca ctcagcgccg ccgcttcact cagtttgcca actcccttcc
     4441 ttcgatcatc tggacagcag agccgagcgg agagatcgac tacttcaacg atgcggtcg
//
LOCUS       DASVKP010000215         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_891199, whole genome shotgun sequence.
ACCESSION   DASVKP010000215 DASVKP010000000
VERSION     DASVKP010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891199"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..297)
                     /gene="rsrA"
                     /locus_tag="VF362_04290"
     CDS             complement(55..297)
                     /gene="rsrA"
                     /locus_tag="VF362_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HEX7590180.1"
                     /translation="MSGKSCEEALDRLYEYIDNELPPEDLRRVAEHLQNCTNCDSERR
                     VNERIKELVAACPKEEAPDDLRVRVLAVIAEARGTG"
     gene            complement(287..946)
                     /locus_tag="VF362_04295"
     CDS             complement(287..946)
                     /locus_tag="VF362_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7590181.1"
                     /translation="MDAAIGTDEYSAEAEAFEALALSFMGQLYAAALRMTRNPADAED
                     LVQETYAKAFAGRDKFTTGTNLKAWLYRIQTNAFINTYRKKQREPKRSDAEAVEDWQI
                     ARAASHTSLGLVSAEQAALDSIGDLDIREALAALPEDFRLAVYLSDVEGFAYKEIAEI
                     MDTPVGTVMSRLHRGRKLLRELLADYAIDRGIVNHRGVVTESAGVTDRAGTGEGGSDV
                     R"
     gene            1151..1636
                     /locus_tag="VF362_04300"
     CDS             1151..1636
                     /locus_tag="VF362_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590182.1"
                     /translation="MFIRTVARPLLASWFVYAAVDAILDPGRHAKKTAPLVEPYLEEA
                     GITLTVPQLARIHGALTLVAATALAFSRTPRTSGLALAGLATVTVATGEPFWRETNEA
                     ARSASRENFVKNISLLGGLLIAASAGGSGTQRRRVKARAKRRIAKASFAKSTGSKKSR
                     R"
     gene            1633..>2856
                     /gene="aroA"
                     /locus_tag="VF362_04305"
     CDS             1633..>2856
                     /gene="aroA"
                     /locus_tag="VF362_04305"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX7590183.1"
                     /translation="MTPTTGTPGVGPGQLWDAPVAKGPLDATVEVPGSKSLNSRFLVL
                     SAIASGPVTIRGALRSRDSSLMIEALRNMGVVVDDSGPDWTVTPPARLTGGGFVECGL
                     AGTVMRFVPPLVLLADATVTFDGDAGAHDRPMGPMIDALRALGATVDDAGTGTMPFSV
                     TPPTEVAPEVSIDASASSQFVSGLLLVAARLPRGLTIRHTGETLPSLPHIDMTCETLR
                     GAGIEVLQPDERTWTVLPGQIHLGEVRVEPDLSNAGPFIAAALVAGGTVRIPHWPAET
                     TQAGALYPELLRTMGATCTLADGVMTVTGTGSIHGIDADLSPAGEITPTIAALCALAD
                     GPSNLMGIGHLRGHETDRLAALATEIERIGGRCDEGPASLHIWPPIEGALRGAEMQTY
                     HDHRMATFGGIVGLRV"
BASE COUNT          403 a         1070 c          958 g          425 t
ORIGIN      
        1 aggacctccc gccatccgaa acggacccag ccgtcgggcg ggaattcggg ctaactatcc
       61 ggtcccgcgt gcctccgcga tcactgcgag cacgcgcacg cgcaggtcgt cgggagcctc
      121 ctccttgggg caggcggcga cgagctcctt gatccgctcg ttgacccggc gctcggagtc
      181 gcagttcgtg cagttctgca ggtgctcggc cacgcggcgc aggtcctcgg gcgggagctc
      241 gttgtcgatg tactcgtaga ggcgatccag cgcctcctcg cacgacttac cggacatcgg
      301 agcccccctc ccccgtgcca gcgcggtcgg tcacgccggc gctctcggtg acgacgccgc
      361 ggtggttgac gatgccgcgg tcgatcgcgt agtccgcgag cagttcgcgg aggagcttcc
      421 tgccgcggtg aagccgggac atcacggtcc ccacgggggt gtccatgatc tcggcgatct
      481 ccttgtaggc gaagccctcg acgtcgctga ggtacacggc cagccggaag tcctcgggga
      541 gcgcggcgag cgcctcgcgg atgtccaggt ccccgatcga atcgagcgcg gcctgctccg
      601 cggagacgag ccccaacgac gtatgggacg ccgcccgcgc gatctgccag tcctcgaccg
      661 cctcggcgtc gcttctcttg ggctcccgct gcttcttgcg gtacgtgttg atgaaggcgt
      721 tcgtctggat ccggtagagc caggccttga ggttagtgcc cgtggtgaac ttgtcccgcc
      781 cggcgaaggc cttggcgtac gtctcctgga ccaggtcctc agcatcggcg gggttgcgcg
      841 tcatgcgtag cgcggcggcg tagagctggc ccatgaagga cagcgcgaga gcctcgaagg
      901 cctcagcctc cgcggagtat tcgtcggttc cgatcgcggc gtccatcgcc cacgagccta
      961 gtgggccgac ggcggggcgt gcgccgacgt cacggggacg ggcctcgagg cagacggtca
     1021 tatgggtgca acggcgcgcg cccgacgccc attcccgacg ccccttcccg aagcccttcc
     1081 cgacgcccgg ggacgacgga tcgtgcgggg ttggcaccga cggcggtaag ttgtaaccaa
     1141 aggaggcatc atgttcattc gcaccgtcgc ccggcccctc ctcgcgtcgt ggttcgtcta
     1201 cgcggccgtg gacgccatcc tcgatccggg tcgccacgcc aagaagaccg ctcccctggt
     1261 ggagccctac ctcgaggagg cgggcatcac cctgaccgtc cctcaactcg cgcgaatcca
     1321 cggcgccctg accctcgtcg cggccaccgc cctggccttc tcgcgcacgc cccgcacctc
     1381 aggcctcgca ctcgcaggcc tcgccacggt gaccgtcgcg acgggcgaac ccttctggcg
     1441 ggaaacgaac gaggccgcgc gctccgcgag ccgcgagaac ttcgtcaaga acatctccct
     1501 cctcgggggc ttgctcatcg ccgccagtgc cggggggtcg gggacccagc ggcgcagggt
     1561 caaggcgcgc gccaagcgcc gcatcgccaa ggccagtttc gccaaaagca caggctccaa
     1621 gaagtcccgt aggtgacccc caccaccgga acgccgggcg tcggacccgg ccagttgtgg
     1681 gacgccccgg ttgccaaggg cccgctcgac gccaccgtcg aggtccccgg tagcaagtcc
     1741 ctcaatagcc gcttcctcgt cctctccgcg atcgcgagcg ggcccgtgac gatccgcggc
     1801 gccctgcgct cgcgcgactc gagcctcatg atcgaggcgc tgcgcaacat gggcgtcgtc
     1861 gtggacgatt cggggccgga ctggacggtc acgccgcccg cgcgcctcac cgggggcggc
     1921 ttcgtggagt gcggcctcgc gggcacggtg atgcgcttcg tcccgcccct cgtcctcctg
     1981 gccgacgcga ccgtgacctt cgacggcgac gccggggcgc acgaccgtcc gatgggcccg
     2041 atgatcgatg cgcttcgtgc gctcggcgcg acggtggacg acgccgggac cggcaccatg
     2101 cccttctccg tcacgccgcc aacggaggtc gccccggagg tctccatcga cgcctcggcg
     2161 tcgtcccagt tcgtctccgg cctgcttctt gtcgccgcgc gccttccccg cggcctgacc
     2221 atccggcaca cgggcgagac gcttccgagc cttccccaca tcgacatgac gtgcgagacg
     2281 ctccgcggcg cgggcatcga ggtgctccag ccggacgagc gcacgtggac cgtgctcccc
     2341 ggacagatcc acctgggaga ggtgcgggtc gagcccgacc tgagcaacgc cggccctttc
     2401 atcgccgccg cgctcgtcgc gggcgggacg gtgcgcatcc cccactggcc cgccgagacc
     2461 acgcaggcgg gggccctcta ccccgaactc ctgcgcacga tgggcgccac ctgcacgctc
     2521 gcggacggcg tcatgaccgt caccggcacg ggcagcatcc acgggatcga cgccgacctg
     2581 agccccgcgg gcgagatcac ccccacgatc gccgcgctct gcgcgctcgc cgacggcccc
     2641 tccaacctga tgggcatcgg ccacctgcgc ggccacgaga cggaccgtct cgcggcgctc
     2701 gccacggaga tcgagcggat cggcggccgg tgcgacgagg ggcccgcgtc tctgcacatc
     2761 tggcccccca tcgagggcgc gctccgcgga gcggagatgc agacgtacca cgaccaccgc
     2821 atggcgacct tcggggggat cgtcggcctg cgcgtc
//
LOCUS       DASVKP010000216         3234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1546419, whole genome shotgun sequence.
ACCESSION   DASVKP010000216 DASVKP010000000
VERSION     DASVKP010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546419"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VF362_04310"
     CDS             <1..198
                     /locus_tag="VF362_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX7590184.1"
                     /translation="FALQLCQGHCPVQSVAEAAPEWCEEETRVLARLLGVHVQRLSTL
                     AGGAHVCTTNIPLAVAGKEGS"
     gene            195..1628
                     /gene="sufB"
                     /locus_tag="VF362_04315"
     CDS             195..1628
                     /gene="sufB"
                     /locus_tag="VF362_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX7590185.1"
                     /translation="MTTGTEKTPLTQEETIASIGTYGYGWHDSDTAGMSAQRGLSEAV
                     VRNISALKSEPEWMLKTRLKGLDFFGKKPMPPWGADLSGIHFDNIKYFVRSTEKQATS
                     WDELPDDIKATYDRLGIPEAEKQRLVAGVAAQYESEVVYHQIREDLEKRGVLFLDTDT
                     ALREHPEIFREYFGTVIPAGDNKFAALNTAVWSGGSFVYVPPGVHVDIPLQAYFRINT
                     ENMGQFERTLIIADEGSYVHYVEGCTAPIYSSDSLHSAVVEIIVKKNARVRYTTIQNW
                     SNNVYNLVTKRATAGEGATMEWVDGNIGSKVTMKYPAIYLLGEHARGETLSIAFAGAG
                     QHQDAGAKMVHAAPHTSSSIISKSVARGGGRTSYRGLVQILEGAHGSKSNVLCDALLV
                     DQISRSDTYPYVDVREDDVSMGHEATVSRVSEEQLFYLMSRGMPETEAMAMIVRGFVE
                     PIARELPMEYALELNRLIELQMEGAVG"
     gene            1625..2827
                     /gene="sufD"
                     /locus_tag="VF362_04320"
     CDS             1625..2827
                     /gene="sufD"
                     /locus_tag="VF362_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX7590186.1"
                     /translation="MSPAETLATDHSKAVADGSHTHGTGPGFAVPAKSRAERLTSFDP
                     ADFPMPTGREEDWRFTPLARLGSALNDVPVENSPTLTLDTLPPGVTVTQIDAAAARSR
                     SVRKASDRAGAIAASRAPRGVLMSVARGAIVSRPVEIGGALKGGPFRTHFVLEVGEGA
                     EVTVIARYTGAAEISEFLSIDLAARARLNLVLIDECSSEAIHLTDLVVRVGKDAHLRG
                     SIVTLGAGLARTNATVEMAGEGSEVDLIGLYFADSGQHLENRVFIDHVAPRARSRVAY
                     KGALAGQTARTIWIGDVLIRAAAEGTDTYELNRNLLLTEGARADSVPNLEIETGEIAG
                     AGHASATGRFDDEQMFYLQSRGIPEDEARRMVVRGFFADLVRQIGVASVEQGLMRAID
                     RELGVAHE"
     gene            2820..3176
                     /locus_tag="VF362_04325"
     CDS             2820..3176
                     /locus_tag="VF362_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX7590187.1"
                     /translation="MSEHVACKAKALAPGTALRADLALATGGKHAFAVVRTEDGELFA
                     IDDTCSHAEVSLSEGDVDGCEIECWAHGSRFDLRTGEPDELPAMTPIRTYPVRIDGDD
                     VLVDVDAPMSTAKENA"
BASE COUNT          552 a         1154 c         1068 g          460 t
ORIGIN      
        1 ttcgccctcc aactttgcca ggggcactgc ccggtccaat cggtcgccga ggcggcgccc
       61 gagtggtgcg aggaggagac ccgcgtcctc gcgcgcctcc tcggggtcca cgtccagcgc
      121 ctgtccaccc tcgcgggcgg cgcgcacgtc tgcaccacca acattccgct cgccgtggcg
      181 ggcaaggagg gctcatgact acaggcacgg agaagacccc gctgacgcag gaggagacga
      241 tcgcgtcgat cggcacctac ggctacggct ggcacgacag cgacacggcg ggcatgagcg
      301 cgcagcgcgg cctgagcgag gccgtcgtca ggaacatctc cgccctcaag agcgagccgg
      361 agtggatgct gaagacccgg ctcaagggcc tcgacttctt cggcaagaag cccatgccgc
      421 cgtggggcgc ggacctgagc ggcatccact tcgacaacat caagtacttc gtgcgatcca
      481 ccgagaagca ggcgacgtcg tgggacgaac tccccgacga catcaaggcg acgtacgacc
      541 ggctgggcat ccccgaggcg gagaagcagc gcctcgtcgc tggcgtcgcg gcccagtacg
      601 agtccgaggt ggtctaccac cagattcgcg aggacctcga gaagcgcggc gtgctcttcc
      661 tcgacaccga caccgcgctc cgcgagcacc ccgagatctt ccgggagtac ttcggcaccg
      721 tcatcccggc gggggacaac aagttcgccg cgctcaacac cgcggtgtgg tcgggcggct
      781 cgttcgtcta cgtcccgccg ggggtccacg tggacatccc gctccaggcc tacttccgca
      841 tcaacacgga gaacatgggc cagttcgagc ggacgctcat catcgccgac gagggttcat
      901 acgtgcacta cgtcgagggc tgtacggcgc cgatctactc ctccgactcg ctccacagcg
      961 cggtcgtgga gatcatcgtc aagaagaacg cgcgcgtccg ctacacgacc atccagaact
     1021 ggtcgaacaa cgtctacaac ctcgtgacca agcgggcgac cgcgggcgag ggcgcgacga
     1081 tggagtgggt cgacggcaac atcggctcca aggtgaccat gaagtacccg gccatctacc
     1141 ttctgggcga gcacgcgcgc ggcgagacgc tctcgattgc cttcgcgggt gcgggccagc
     1201 accaggacgc gggggcgaag atggtccacg cggcgccgca cacctcctcg agcatcatct
     1261 ccaagtccgt ggcgcgcggg ggagggcgga cctcctacag gggcctcgtc cagatcctcg
     1321 agggcgcgca cggctccaag tccaacgtgc tgtgcgacgc gctcctggtg gaccagatct
     1381 cgcgctcgga cacgtacccg tacgtggacg tccgcgagga cgacgtcagc atgggccacg
     1441 aagccacggt gtctcgcgtg agcgaggagc aactcttcta cctcatgagc aggggaatgc
     1501 cggagaccga ggcgatggcc atgatcgtcc gcggcttcgt cgagcccatc gcgcgcgagc
     1561 tgcccatgga gtatgcgctc gagctcaacc gcctcatcga actccagatg gaaggagccg
     1621 tcggatgagc ccagcagaga ccctcgcgac ggaccacagc aaggcggtgg ccgacgggtc
     1681 gcacacgcac ggcaccggtc caggcttcgc ggttcccgcg aagtcgcgcg ccgagcgcct
     1741 cacgtcgttc gatcccgcgg acttccccat gccgacgggg cgcgaagagg actggcgctt
     1801 cacgcccctc gcgcggctcg gtagcgcgct caacgacgtc cccgtcgaga actcgcccac
     1861 cctgacgctc gacaccctcc ctccgggcgt caccgtcacg cagatcgatg ccgccgccgc
     1921 acgctcgcgt tccgtccgca aggcgagcga ccgggcggga gcgatcgcgg cgtcccgcgc
     1981 gccgcgcggc gtcctgatgt ccgtcgcccg cggggccatc gtctcgcggc ccgtcgagat
     2041 cggcggtgcg ctcaagggtg gcccgttccg cacgcacttc gtgctggagg tgggcgaggg
     2101 cgcggaggtc accgtcatcg cccgctacac gggtgccgcc gagatctccg agttcctctc
     2161 gatcgacctc gcggcgcgcg cccgcctcaa cctggtcctg atcgacgaat gctcgtccga
     2221 ggcgatccac ctgaccgacc tcgtcgtccg cgtcggaaag gacgcccacc tgcggggctc
     2281 gatcgtcacg ctcggggcgg gcctggcccg cacgaacgca accgtcgaga tggcggggga
     2341 ggggtccgag gttgacctca tcgggctcta cttcgcggac tccggccagc acctggagaa
     2401 ccgcgtcttc atcgaccacg tcgccccgcg cgcccgctcg cgcgtcgcct acaagggcgc
     2461 ccttgccggc cagaccgcgc gcaccatctg gatcggcgac gtcctgatcc gcgccgcggc
     2521 cgagggcacg gacacgtacg aactcaatcg caacctgttg ctgaccgagg gcgcgcgcgc
     2581 cgactccgtc cccaacctcg agatcgagac gggcgagatc gccggcgcgg gtcacgcctc
     2641 ggcgacgggt cgcttcgacg acgagcagat gttctacctc cagtcccggg ggatcccgga
     2701 ggacgaggcg cgcaggatgg tcgttcgcgg cttcttcgcc gacctggtgc gccaaatcgg
     2761 cgtcgcgagc gtcgagcagg gcctgatgcg cgccatcgat cgagagttgg gagtcgccca
     2821 tgagtgagca cgtcgcctgc aaggccaagg ccctcgcgcc gggcaccgcg ttgcgcgccg
     2881 acctcgcact ggctaccggg ggcaagcatg ccttcgcggt cgtccggacc gaggacgggg
     2941 agctcttcgc gatcgatgac acctgttcgc acgccgaggt ctcgctatcc gagggggacg
     3001 tcgacggctg cgagattgag tgctgggccc acggcagtcg attcgacctt cgcaccggcg
     3061 agccggacga gctccctgcc atgaccccca tccgcaccta ccccgtccgc atcgatggcg
     3121 atgacgtcct agtggacgtc gacgccccca tgagcaccgc caaggagaac gcatgagcac
     3181 cctcgagatc cgcggactca aggtcagcgt cgacaccaag gagggcacca agcc
//
LOCUS       DASVKP010000217         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_561491, whole genome shotgun sequence.
ACCESSION   DASVKP010000217 DASVKP010000000
VERSION     DASVKP010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561491"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VF362_04330"
     CDS             complement(<1..669)
                     /locus_tag="VF362_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate-flavodoxin oxidoreductase"
                     /protein_id="HEX7590188.1"
                     /translation="MTAVKTTPAAKTSVAKPKYPGRETVINGNGAVAHVMQHVCDGVI
                     GYPITPSTEIAELFEYARSEGGINVWGRHAFFVETEGEHSAQSGALGASLTGGQYISN
                     ASSSQGLLYAMESHFVTVGKKVGGFVLQVAARVVSKHSLNVMAGHDDVYALLPSGYTI
                     LFGSNPQEAADLAAISYRTSALSLIAVANAMDGFATSHVMSEACMPEPELLREYLGDP
                     SGRIA"
     gene            1126..2109
                     /locus_tag="VF362_04335"
     CDS             1126..2109
                     /locus_tag="VF362_04335"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HEX7590189.1"
                     /translation="MEDLNQRIAAWREARLLTEKQAAAIAAFEASRTPAPGAPVAAQE
                     DGTGVLLAEAIGYVGAAIAVAAVVTLLATGNRWDSLNLGGRLGIVGLLTLIVSGAGLA
                     LRASQRSPIRRLVSLLLSSGVLGVAWMVGILANDQLRWESSSVVLAVGIAALVPAAIV
                     YRWRNRALPQLVILASLLVIVGALLGRPGVDATGAWIGLSMWAVGAAWALLGLGRSLA
                     PDRVAVGFGSVVALVSAQIGSLDRERLALLAVGLVTAALLMVRGVAKATTYLVTIGAV
                     GVLVFLPQAVVELFGATVGALATMLVVGLLLVLASVRLARGRSAKEGGKGQ"
BASE COUNT          295 a          716 c          826 g          424 t
ORIGIN      
        1 ggcgatgcgt ccggaggggt cgccgaggta ctcccggagg agctccggct ccggcatgca
       61 cgcctcgctc atgacgtggc tcgtggcgaa tccgtccatc gcgttggcga cggcgatgag
      121 ggagagcgca gaggtgcggt acgagatcgc cgcgaggtcg gccgcctcct gggggttgga
      181 gccgaagagg atcgtgtagc ccgacggcag gagggcgtag acgtcgtcgt ggccggccat
      241 gacattgagc gagtgcttgg agacaacgcg cgcggcgacc tggagcacga aaccgccaac
      301 cttcttaccg acggtgacga agtgggactc catcgcgtag agcaggccct ggctcgacga
      361 cgcgttggaa atgtactggc ctcccgtgag cgaggcgccg agagcgccgc tctgggccga
      421 gtgctccccc tcggtctcga cgaagaatgc gtgacggccc cagacgttga tgccgccctc
      481 ggaacgcgcg tactcgaaga gctcggcgat ctccgtcgag ggggtgatcg ggtagccgat
      541 gacgccgtcg cagacgtgtt gcatgacgtg cgcgaccgcg ccgttgccgt tgatcacggt
      601 ctcgcgaccg gggtacttcg gcttggctac ggaggtcttg gcggcgggcg tggtcttgac
      661 ggcggtcata gcgtgactcc tgaggggtga acaagccggc gcgaggtctt cggcgggcgc
      721 gtcgctgctc ggctcttccc ggtcggatag accagggtcg gcgcggtggc gaggggggct
      781 gcaagggaca tgtggaggaa ttcgggccca ttttgggcag gcaataccca ggtggcggtc
      841 aacataccct ggccgccttg gccatgtttg gggcgtggaa cacctctgtt ccctcctgtg
      901 catatccatg ctcagcccgg ccggagaggg gccgacagga cgaaggtccc ttgttggggg
      961 tggccgaccc tccattctag gtgaggtttg cctaactagc agcgtgtcgg ggcgttttgg
     1021 ggccgctcaa tgacgctttc gacgcttttg gagtccgagc cgagtgcgta gagttgttcc
     1081 gtcgacgcgc gtggtcgcgg catctcggcg gatcgagggg gcgtcatgga ggacctcaac
     1141 cagcgcatcg cggcgtggcg cgaggcgagg cttctcacgg aaaagcaggc cgccgcgatc
     1201 gccgcgttcg aggcatcgcg gactcccgca ccgggtgccc ccgtggccgc ccaggaagac
     1261 ggaacgggcg tccttctcgc cgaggcgatc gggtacgtcg gcgcggccat cgccgtcgcc
     1321 gcggtcgtta ccctgcttgc gaccgggaac cgctgggaca gcctcaacct tggcggccgt
     1381 ctcggcatag tgggactgtt gacgctgatt gtctcgggcg cgggactcgc tctccgggcc
     1441 tcccagcgct cgccgattcg ccgcctcgtc agtctgctgc tctcatccgg agttctgggt
     1501 gtcgcgtgga tggtcgggat tctcgccaac gaccagttgc gctgggagtc ttcgtcggtc
     1561 gtgcttgccg tgggaatcgc cgcgctcgtc cccgccgcga tcgtgtatcg ctggcgcaat
     1621 cgcgcgctac cgcaacttgt catcctcgcg agcctcctgg tcatcgtcgg ggctttgctt
     1681 ggccgtcccg gagtcgacgc gacgggggcg tggatcgggc tgtcgatgtg ggcggtcgga
     1741 gccgcgtggg cgctactcgg acttggccgc tcgcttgcgc ccgaccgggt cgccgtcggc
     1801 ttcggctccg tggtcgccct cgtttctgct cagatcggtt ccctggaccg cgagcgtctc
     1861 gccctcctcg cggtggggct cgtgacggcc gcgctcctca tggtgcgcgg ggtcgccaag
     1921 gccaccacgt acctcgtgac cattggcgcg gtgggcgtcc tggtcttcct tccccaggcc
     1981 gtcgtcgaac tcttcggggc gacggtcggc gccctggcaa cgatgctcgt cgtgggactc
     2041 ctcctcgtcc tcgcctcggt gcgcctggcg cgcgggcggt ctgcgaaaga gggagggaag
     2101 gggcaatgat gtccgaagtc gcgcctcggt cgaagggcaa cgcggcatac ctgatcgccg
     2161 cggcgatcct cgtcattgtg gtcggcgtcg cggtcgcggt ctttggggtc gtccgcccgc
     2221 ccgcgctggc ctcgatgtcg tcggcggggt tcacgccacc c
//
LOCUS       DASVKP010000218         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1468232, whole genome shotgun sequence.
ACCESSION   DASVKP010000218 DASVKP010000000
VERSION     DASVKP010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468232"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..528
                     /locus_tag="VF362_04340"
     CDS             304..528
                     /locus_tag="VF362_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB
                     C-terminal domain-containing protein"
                     /protein_id="HEX7590190.1"
                     /translation="MPRLDPLPAQAGTPPHLIVGVEGEGDLKDAMYDASSASDAGDDP
                     KSLVDFVVVTPDSDGIAAWLYKNGRKFYEA"
     gene            complement(534..767)
                     /locus_tag="VF362_04345"
     CDS             complement(534..767)
                     /locus_tag="VF362_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590191.1"
                     /translation="MSVESPFAEVGNVDLGKALDEVGNLVCGGLSKVNTMVTLKHSIP
                     LENLNINELQRPLARVVNSADALEKTLVGGDKF"
     gene            complement(832..1500)
                     /locus_tag="VF362_04350"
     CDS             complement(832..1500)
                     /locus_tag="VF362_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590192.1"
                     /translation="MKIRTFLASRAVPASVAVAGVVLLTACGGSPLAATDTSGAYPTY
                     TVQPTEAAVVNPDLTPPGTILAVGESALIGYHTLVPDASGAAAPSKLEQPVHVTVTGV
                     SSGSMSQVPDAFRLTGADAGTENMSVKKVRWEATIDGTSVLALADQTLNFTATGGGSY
                     IVSDPSAISGCKGPATLGAAFDSGSTVTGCFLIVYLAASGTPDVELSVFRTPTEGNPI
                     HWKS"
     gene            complement(1876..>2211)
                     /locus_tag="VF362_04355"
     CDS             complement(1876..>2211)
                     /locus_tag="VF362_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590193.1"
                     /translation="CDGGPDFGTPVEVTNRCDVDLAIAFQTEASPGPTRSTTKEASRL
                     KSGESSGWLLAPITGYSHGIQYMWVTNNGSATWGDPVEVDLRTLPEGDLSGGQAERVL
                     VIEGDLCPR"
BASE COUNT          327 a          742 c          801 g          341 t
ORIGIN      
        1 ctcctcggcg gagtggctcg cggccgtggt gaagcgacag gttccgatcc tccacctgcc
       61 cacccgcgcg ctgttcgagg ggacgaaggg cgcgacgctc gcgctcaacc tgggcgcccc
      121 gctggccaag gagttcagcc cgggcgagat cgcgagcctc cttggcggcg gtgatggcga
      181 ggccgccgcg aacgcgctag gcaacccgag cgccgtggtg atcagccttc ccgaggtcga
      241 gccgctcgcc tacctcgccg ccgcgacgac ggtcttctcg cgccaccccg aggtgcgggc
      301 ggcgtgcctc ggctggatcc actacccgca caggccggaa cccctccgca cctcatcgtc
      361 ggggtcgagg gcgagggcga cctcaaggac gcgatgtacg acgcgagttc cgcctcggac
      421 gcgggcgacg acccgaagag tctggtggac ttcgtggtcg tcacgccgga ctcggacggc
      481 atcgcggcgt ggctgtacaa gaacggcagg aagttctacg aggcgtaggc gccttagaac
      541 ttgtcgccgc ccaccagcgt cttctccagc gcgtcggcgg aattgacgac ccgggccagg
      601 ggccgctgaa gctcgttgat gttgaggttc tccagcggga tggagtgctt gagcgtcacc
      661 atcgtgttga ccttggaaag ccctccgcac acgaggttcc cgacctcgtc gagcgccttg
      721 ccgaggtcaa cgttgccgac ctccgcgaac ggtgactcga ccgacaccca gtccgcggcg
      781 cccggctgcg gccccgctcg gcggaccgcc gcggagcagc gcggcgagag gctacgactt
      841 ccagtggatc gggttgccct ccgtcggagt ccggaagacg gacagttcga cgtccggcgt
      901 gccgctggcg gcgaggtaga cgatcaggaa gcatcccgtg accgtggagc cggaatcgaa
      961 cgccgcgccg agggttgccg ggcccttgca ccccgagatc gcgctcgggt ccgacacgat
     1021 gtaggagccg ccgccggtcg ccgtgaagtt gagggtctgg tccgcgagcg caagcacgga
     1081 cgtcccgtcg atcgtggcct cccacctgac cttcttgacg gacatgttct ccgtaccggc
     1141 gtcggcgccc gtcaggcgga aggcgtccgg gacctggctc atgctcccgc tcgagacccc
     1201 cgtgacggtc acgtggacgg gctgctccag cttggacgga gcggcggcgc ccgatgcgtc
     1261 ggggacgagc gtgtgatacc cgatcagggc gctctcgccg acggcgagga tcgttccggg
     1321 gggcgtgagg tcggggttga ccacggcggc ctcggtcggc tgcaccgtgt aagtcgggta
     1381 ggcaccggac gtgtcggtcg cggcgagcgg gctgccgccg caggcggtca ggaggacgac
     1441 gccggcgacg gcgacgctgg cggggacggc gcgtgaggca aggaaggtcc ggatcttcaa
     1501 ggtttcactc ctcggggcaa cgcgcgggac aaccccgcgt cgaaaatgga cattaccagc
     1561 gaccggaggg gtcggggcgc cacggggcgg tcggtcggct agccgtagcg cgcgggctcc
     1621 gcgccctcga cggtgatgct cccgtcgtcg gcgacgggcc acccggggtt gtgggcgaga
     1681 gcggcacgag cttggcgacc tccgtcccgc cgcgcgagac gatcaggcct tgggggtgct
     1741 gggcgagcga gcccaacacg cgcgaggtct cgcgcgcgag agtccgtgcc gtgatcgtgt
     1801 ttgccatgtc tgacattcga ggcgatgcgt ctgacacggc tcggcgctga tgcgaggcgc
     1861 gcgatcccgg cgcggtcacc taggacacag gtcgccttcg atcacgagga ctctctcggc
     1921 ctgcccacca gagaggtccc cttcgggcag agtccgcaag tcgacttcga cgggatctcc
     1981 ccacgtggcc gagccgttgt tggtcaccca catgtactga atgccgtggg agtagcccgt
     2041 gatcggggca agtagccacc cgctcgactc cccggacttg aggcgtgagg cctctttggt
     2101 cgtgctccgt gtcggaccgg gcgacgcctc cgtctggaac gcgatcgcta ggtcgacgtc
     2161 gcagcggttg gtcacttcga cgggcgtccc gaagtccggc cccccgtcgc a
//
LOCUS       DASVKP010000219         4099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1223894, whole genome shotgun sequence.
ACCESSION   DASVKP010000219 DASVKP010000000
VERSION     DASVKP010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223894"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VF362_04360"
     CDS             <1..1174
                     /locus_tag="VF362_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7590194.1"
                     /translation="VLAHAQLVKDRGAAAHAEWDAKFGTWRAAQVERAALLDRLIKRD
                     LPKGWTASLPVFEAGKSIATRAASGEVLTALAATLPELWGGSADLAGSNNTTMKGEPS
                     FLPFERSSKAFPGNPYGRTLHFGIREHAMGMILNGITLHGLTRPYGGTFLVFSDYMRG
                     AVRLAALQKIPATFVWTHDSIGLGEDGPTHQPVEHLSALRAIPGLDIVRPADANETAW
                     AWRGILERTDRPAGIILTRQNVPTFDRTTVDGTALGSVEGTLKGAYVLADTDGTPDVL
                     IIATGSEVQLALGARDILAGEGVKARVVSMPCREWFCDQDAAYRESVIPAGVRARVTV
                     EAGIAQCWNDILGDAGRAVSIEHFGESADGDYLFEKYGFTADNVAAKARESIKAAL"
     gene            1171..2793
                     /locus_tag="VF362_04365"
     CDS             1171..2793
                     /locus_tag="VF362_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019483799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX7590195.1"
                     /translation="MSAFVTAWAGLGDEAHVSVAASGAATEAVATHLEALVSDQVASR
                     IAAKDATLWGAEAESEASVRLGWTELHVTTRALLPQLAELTAALRAEGKTRVVLCGMG
                     GSSLGPEVIARTYEVPLVVLDSTDPDQVRGALAGDLASTVVVVSSKSGGTVETDSQRR
                     AFEAAFTEAGLDPRQHIVIVTDPGSPLETASREAGYRAIFLADPNVGGRFSVLSAFGV
                     VPSALAGVDVEGLLDEAAAVAPSFGEDDASNPALVLGAAMCAAGKDKLVFRDDASGLV
                     GLPDWAEQLIAESTGKNGVGLLPVVVEGSAPEVGQPDVADVRIAALDAIPEGDIAVSG
                     TLGGQFLLWEYATAIAGRILGINPFDQPNVESAKIAARGLLDATPEPGPAAFIDEGIE
                     VRAIPASLLDDVDTVDAALAALEATLGDRGYVAVMAYLDREEFGPFAEVRDAVARRTS
                     RPVTFGWGPRFLHSTGQLHKGGAPVGVFLQITGTFATDLAIPDRPFSFGTLIAAQALG
                     DAVVLAEHDRPVLRLTLTEASQLPRLRELLEG"
     gene            2796..>4099
                     /gene="zwf"
                     /locus_tag="VF362_04370"
     CDS             2796..>4099
                     /gene="zwf"
                     /locus_tag="VF362_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX7590196.1"
                     /translation="MTNPLRDPRDRRLPRIAGPCGLVMFGVTGDLAKRKLMPAIYDLA
                     NRGLLPPAFALTGFARREWDRQDFAKVVYDSVREHARTPFREETWEQLSAGIRFVEGT
                     FDDPQAFERLRATVEELDEKRGTGGNHAFYLSVPPSSFPLVCRQLADSGLSKPREGTW
                     RRVVIEKPFGHDLASAKALNDVVSEVFPSDAVFRIDHYLGKETVQNLLAIRFANQLFE
                     PLWNSQYVDHVQITMAEDAGIGSRAGYYDGIGAARDVIQNHLMQLLALTAMEEPATFE
                     AADLRMEKEKVLRSVRTPRDLTTGTVRGQYAAGWRGGEQVGGFLDEEGIPGDSVTETY
                     AALRLDIDNRRWAGTPFYLRAGKRLGRRVTEIALVFKKVPHFPFPIDSEAEVGNNALV
                     IRVQPDEGVTLRFGSKVPGTSMEIRDVTMDFGYGSAFNETSP"
BASE COUNT          617 a         1505 c         1368 g          609 t
ORIGIN      
        1 ggtccttgcg cacgcccagc tcgtgaagga ccgcggcgcc gcggcccacg ccgagtggga
       61 cgccaagttt ggcacgtggc gggcggcgca agtcgagcgc gcggcgctcc tcgaccggct
      121 catcaagcgc gaccttccga agggctggac cgcgtcgctg ccggtcttcg aggcgggcaa
      181 gtccatcgcg acccgtgcgg cctcgggcga ggtcctcacc gcgcttgcgg cgacgctgcc
      241 cgagctctgg ggcggctccg cggacctcgc gggttcgaac aacacgacga tgaagggcga
      301 gccgtcgttc cttcccttcg agcgatcctc caaggcgttc cccggcaacc cctacggacg
      361 caccctccac ttcggcattc gcgagcacgc catgggcatg atcctcaacg gcatcacgct
      421 ccacggcctc acgcgcccct acggcggcac gttcctggtg ttcagcgact acatgcgagg
      481 cgcagtccga ctcgccgcgc tgcagaagat cccggcgacg ttcgtatgga cccacgactc
      541 gatcggcctc ggagaggacg ggccgaccca ccagcccgtc gaacacctct ccgcgcttcg
      601 cgcgatcccc gggctcgaca tcgttcggcc ggccgacgcg aacgagacgg catgggcgtg
      661 gaggggaatt ctcgaacgca ccgaccgccc cgcgggcatc atcctgacgc ggcagaacgt
      721 tccgacgttc gaccgcacga ccgtagacgg caccgcgctc ggctcggtcg agggcaccct
      781 taagggcgcc tacgtcctcg ccgacaccga cgggacgccg gatgtcctga tcatcgcgac
      841 gggctccgag gtccagctgg ccctcggggc tcgcgacatt ctcgcgggcg agggagtgaa
      901 ggcgcgcgtc gtgtcgatgc cctgccgcga gtggttctgc gatcaggacg ccgcctaccg
      961 cgagtccgtc atccccgccg gggtcagggc tcgcgtcacg gtcgaggcgg gcatcgccca
     1021 gtgctggaac gacattctcg gcgacgccgg ccgcgcggtg agcatcgagc acttcggcga
     1081 gtccgcggac ggcgactacc tgttcgagaa gtacgggttc accgccgaca acgtggccgc
     1141 caaggcccgc gagtccatca aggcggcgct gtgagcgcct tcgtcaccgc ctgggcgggc
     1201 ctcggcgacg aggctcacgt ctccgtggca gcttccggcg cggcgaccga ggcggttgcg
     1261 acccacctcg aggccctcgt ctccgaccaa gtcgcctcca ggatcgcggc gaaggacgcg
     1321 accctgtggg gcgccgaggc cgagtccgag gcgtcggttc ggctcggctg gacggaactt
     1381 cacgtaacga cccgcgcgct actcccccag ctcgccgagc tcaccgcggc actgcgcgcc
     1441 gagggcaaga cgcgcgtcgt cctgtgcggc atgggcggat catcgctcgg ccctgaggtc
     1501 atcgcacgga cgtacgaggt gccgctggtc gtcctcgact ccaccgaccc cgaccaggtg
     1561 cgcggcgccc tcgcgggcga cctcgcgtcg acggtcgtcg tcgtgtcctc caagtccggc
     1621 gggaccgtcg agacggactc ccagcggcgc gccttcgagg cggcgttcac cgaggcgggg
     1681 ctcgacccgc gccagcacat cgtcatcgtc acggacccag ggtcgccgct cgagacggca
     1741 tctcgcgagg ccggataccg ggccatcttc cttgccgacc ccaacgtcgg cggccggttc
     1801 tcggtgctca gcgcgttcgg ggtcgtcccg tccgcgctcg cgggggttga cgtggagggc
     1861 ctgctcgatg aggccgccgc ggtggcgccc tcgttcgggg aggacgacgc gtcgaacccc
     1921 gccctcgtgc tcggcgccgc catgtgcgcc gccggcaagg acaagctcgt cttccgggac
     1981 gacgcctccg gcctcgtcgg actccccgac tgggccgagc aactgatcgc cgagtccacg
     2041 ggcaagaacg gtgtgggact cctccccgtc gtcgtcgagg gttccgcgcc cgaagtcggc
     2101 cagcccgacg tcgcggacgt gaggatcgcc gcactcgacg cgattcccga gggcgacatc
     2161 gccgtctcgg gcacgctggg cgggcagttc ctgctgtggg agtacgccac cgcgatcgcg
     2221 ggccgcatcc tcgggatcaa ccccttcgat cagccgaacg tcgagtccgc caagatcgcg
     2281 gcgcggggcc tcctcgacgc gacccccgag ccgggtcccg ccgcgttcat cgacgagggc
     2341 atcgaggttc gcgcgatccc cgcctcgctc ctcgacgacg tcgacacggt cgacgccgcg
     2401 ctcgccgcgc tcgaggcgac cctcggcgac cgcggctacg tcgcggtcat ggcgtacctc
     2461 gaccgggagg agttcggccc tttcgccgag gtgcgggacg cggttgcgcg ccgcacatcg
     2521 cggcccgtca cgttcggttg ggggccaaga ttcctccaca gcaccgggca gttgcacaag
     2581 gggggcgcgc ccgtcggcgt cttccttcag atcacgggca ccttcgccac ggacctcgcg
     2641 atcccggacc ggcccttcag cttcggcacc ctcatcgccg cccaggcgct cggcgacgcc
     2701 gtagtgctgg ccgagcacga ccgacccgtc cttcgcctga ccctgacgga ggcgtcccag
     2761 ttgccgcgcc tcagggaact gctggaaggg taggcgtgac caatcccctc cgcgaccccc
     2821 gcgaccgccg cctcccacgg atcgcggggc cctgcgggct cgtcatgttc ggagtgacgg
     2881 gcgacctcgc gaagcgcaag ctcatgcccg cgatctacga ccttgccaac cgcggcctgc
     2941 tcccgcccgc gttcgcgctc acgggcttcg cgcgccgcga gtgggatcgc caggacttcg
     3001 ccaaggtggt ctacgactcg gtcagggaac acgcgcgcac gcccttccgc gaggagacgt
     3061 gggagcaact cagcgccggg attcgcttcg tcgagggaac gttcgacgac ccccaagcct
     3121 tcgagcgact ccgcgccacg gtggaggaac tcgacgagaa gcgcggcacc gggggcaacc
     3181 acgcgttcta cctctccgta ccgccgagtt ccttccccct cgtatgccgc cagctcgcgg
     3241 actcgggctt gtccaagccg cgagagggaa cgtggcgccg cgtcgtcatc gagaagccct
     3301 tcggccacga cctcgccagc gccaaggcac tgaacgacgt cgtctcggag gtcttcccgt
     3361 cggacgcggt cttccgtatc gatcactacc tgggcaagga gacggtgcag aacctgcttg
     3421 cgatccggtt cgcgaaccaa ctcttcgagc ccctgtggaa cagccagtac gtcgatcacg
     3481 tccagatcac catggcggag gatgccggga tcggcagccg cgccggctac tacgacggca
     3541 tcggggccgc ccgggacgtc atccagaacc acctcatgca gctcctcgcg ctcaccgcaa
     3601 tggaggagcc cgcaaccttc gaggctgcgg acctccgcat ggagaaggag aaggttctgc
     3661 gctcagttcg gactccgcgg gacctgacga cggggacagt ccgcggacag tacgcggccg
     3721 gttggcgcgg gggcgagcag gtcggaggct tcctcgacga ggagggcatc ccgggtgact
     3781 ccgtgaccga gacctacgcg gcactgcggc tcgatatcga caatcgccgg tgggccggga
     3841 ccccgttcta cctccgggca ggcaagcgcc tcgggcgacg cgttacggag atcgcgctcg
     3901 tgttcaagaa ggttccgcac tttcccttcc ccatcgattc cgaggccgag gtgggcaaca
     3961 acgcgctcgt catccgcgtc cagcccgacg agggcgtcac ccttcgcttc ggctccaagg
     4021 ttccgggcac gtccatggag atccgtgacg tgacgatgga cttcggctac ggcagcgcct
     4081 tcaacgagac ctcccccga
//
LOCUS       DASVKP010000220         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1308200, whole genome shotgun sequence.
ACCESSION   DASVKP010000220 DASVKP010000000
VERSION     DASVKP010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308200"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VF362_04375"
     CDS             <1..292
                     /locus_tag="VF362_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590197.1"
                     /translation="TLTAARPQAPVPVLDVALEDANGTERVPLGTLRPEGSAWRAYDL
                     AVPGDFWVRHRGAPVRVVLASDSTWRPADVLPGSGDARELSVEVFRIRFVTE"
     gene            346..1338
                     /locus_tag="VF362_04380"
     CDS             346..1338
                     /locus_tag="VF362_04380"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7590198.1"
                     /translation="MTSETPSFAGACALVTGGAGFIGSTLAIALAERGATVRAVDAMI
                     PGHGGNLFNLEPVKSRVAIDVADIRDQDAMNRAVRGQDIVFHLAGQMDHVLSLTDPFP
                     DIDINIKGTTVLMEALKRHNPAARVVYTGTRGEYGSADSVPVAEDAPTNPRGLHEISN
                     LAAVKIVEMYGRLHGIRGTMLRLSNIYGPRAQMRHSRYGVVNWFVRLALEGSVIPVFG
                     DGKILRDFLYVDDCVEAILLAAGSDAVFGEVLNVGGGEPTTFREVAETLSSLTGARWE
                     FAPFSAERKAQEPGDFYSDISKIRRVLGWRPVTPLREGLQRTLDYYRAHRPRYW"
BASE COUNT          232 a          563 c          502 g          239 t
ORIGIN      
        1 caccctcaca gcggcgcggc cacaggcccc cgtgccggtg ctggacgttg cgctcgagga
       61 cgcaaacggc accgagcgcg tccctctcgg tacgctccgt cccgagggct ccgcgtggcg
      121 cgcctacgac ctcgcggttc cgggggactt ctgggtccgg caccgcggcg cacccgtccg
      181 cgtcgtcctc gcgtcggact cgacgtggcg ccccgccgac gtcctcccgg gctccggcga
      241 cgcccgggaa ctctccgtgg aagtcttccg gatccgcttc gtcacggaat gacgacgcac
      301 gaggaagtgc ggaacgcagg ggcctgacgc tatgctcgca ctccgatgac ctcggagacg
      361 ccgtcgttcg cgggtgcctg cgccctcgtg acgggcggcg ccggcttcat cgggagcacg
      421 ctcgcgatcg cgcttgccga gcgaggcgcc accgttcgcg ccgtcgacgc catgatcccg
      481 ggccacggag gcaacctctt caacctcgag cccgtgaaga gccgggtcgc catcgacgtc
      541 gcggacatcc gcgaccaaga cgcgatgaac cgcgccgtgc ggggccagga catcgtcttc
      601 cacctcgcgg gccagatgga ccacgtcctc tcactcacgg atccattccc cgacatcgac
      661 atcaacatca aggggacgac cgtcctcatg gaggcgctca agcgccacaa cccggccgcg
      721 cgcgtcgtct acacggggac gcgcggcgag tacggcagcg cggattcggt gcccgtggcc
      781 gaggacgcgc ccacgaatcc gcgaggcctc cacgagatct cgaacctcgc cgcggtcaag
      841 atcgtggaaa tgtacggccg cctccatgga atcaggggaa cgatgctgcg cctctcgaac
      901 atctacggcc ctcgggcaca gatgcgccat tcgcgctacg gcgtcgtgaa ctggttcgtg
      961 cgactcgccc tcgaaggttc cgtgatcccc gtcttcggcg acgggaagat cctccgcgat
     1021 tttctctacg tcgacgactg cgtcgaggcc atcctcctcg cggcgggctc ggacgcggta
     1081 ttcggcgagg tcctgaacgt gggcggtggc gagccgacga cgttccgcga ggtcgccgag
     1141 acgctctcgt ccctcacggg ggcgcgctgg gagttcgcgc ccttctccgc cgagcgcaag
     1201 gcgcaggagc ccggcgactt ctactccgac atctcgaaga tccggcgcgt tctcggttgg
     1261 agacctgtca cgcctctgcg cgagggtcta cagcggacgc tcgactacta ccgcgcgcat
     1321 cgtccgcgct actggtaacg cgggccgagg cacgcgactg cgggcgtctt cgttccggtc
     1381 gccctcctca gcgcgcgacg gcgacgtgcg cgtcgagagt cgcgccgccc ttagccgaga
     1441 atcgcgcgat cccctcttgc tcgaggtcga gccgcaacaa gtagttgccg gacgtccccg
     1501 gcgcacggag ccggaaggtc aggtccgccg tctccc
//
LOCUS       DASVKP010000221         4184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_729743, whole genome shotgun sequence.
ACCESSION   DASVKP010000221 DASVKP010000000
VERSION     DASVKP010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729743"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VF362_04385"
     CDS             <1..732
                     /locus_tag="VF362_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX7590199.1"
                     /translation="RQRVLLFGVAVALVLRGIFIVAGAAALSHFSWVFYLFGFFLLWT
                     ALSLAREGHEDEEDVEAEYHENRAMVMVRRLLRSTPEYHGGALVIRRAGRRLVTPLLL
                     VMVAIGTTDVLFALDSIPAIFGLTTDAYIVFAANAFALMGLRQLFFVVHGLLTRLVFL
                     SYGLAAILAFIGVKLVFEALHENTLPFIHGGEPVTGLPTITTELSLGVIMAALGVSAI
                     ASLIATRRRTASAAATGQARDSSTD"
     gene            complement(705..1010)
                     /locus_tag="VF362_04390"
     CDS             complement(705..1010)
                     /locus_tag="VF362_04390"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX7590200.1"
                     /translation="MDLFAVHYAYDDRTEDRDAIRSSHIEYLDALVRQGALFAYGRYA
                     DELEPGALFIYRADAPDAVEAWVAGDPFVVAGLVPRHTVRAWPALGTWPQSVEESRA"
     gene            1094..3934
                     /gene="uvrA"
                     /locus_tag="VF362_04395"
     CDS             1094..3934
                     /gene="uvrA"
                     /locus_tag="VF362_04395"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006887184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX7590201.1"
                     /translation="MNDRLVIRGAREHNLRSIDLDLPRDALIVFSGLSGSGKSSLAFD
                     TIFAEGQRRYVESLSAYARQFLGQMDKPDVDFIEGLSPAVSIDQKSTNRNPRSTVGTI
                     TEVYDYLRLLFARVGIQHCPECGEQVRAQTAEQIVDSVLALPEGTRYQVLAPVVRGRK
                     GEYQDLFDELKQQGFARARVDGEAVELSAPPTLEKQIKHTIEVIVDRLVAREGVRSRL
                     ADSIETALRVAEGLVIIDPVDGEMEARRYSEKRACPNDHPLTLEEIEPRTFSFNAPYG
                     ACPECHGLGVRLEVDPDLVVPDPSLTLRAGALAPWAATSSEYFARTLAALASKLEFSM
                     DVPFSELSDEAKKAVLFGSDYSVHVRFKNRYGRERQYTTGFEGVVSWIERLHSQTESE
                     WSRERHEAYMRDVPCDVCEGKRLKPEVLSVLVAGKSISDVCELSIRGARTFLAEAELD
                     ERSRQIGVQVLKEIDARLGFLLDVGLDYLSLSRAAGTLSGGEAQRIRLATQIGSGLVG
                     VLYVLDEPSIGLHQRDNHRLIETLTRLRDLGNTLIVVEHDEDTIRAADWIVDIGPGAG
                     EHGGRVIHSGTLESLLTNEESLTGAYVSGRLRIDVPAQRREIDPGRMITVVGARENNL
                     RDIDVSFPIGVFTSVTGVSGSGKSTLVNGILYTALANTLNGARQVPGRHTRITGIEDL
                     DKIVHVDQGPIGRTPRSNPATYTGVWDKVRKLFAETEEARLRGYGPGRFSFNVKGGRC
                     ESCSGDGTLKIEMNFLPDVYVRCEVCHGSRYNRETLQVHFKGKTVADVLNMPIEEAAD
                     FFGAIPAISRQLRTLVDVGLGYVRMGQPAPTLSGGEAQRVKLASELQRRSTGRTVYVL
                     DEPTTGLHFDDVRKLLAVLQGLVDKGNTVIVIEHNLDVIKCSDWIVDLGPEGGSGGGI
                     VVAEGTPEDLARDDASHTGRFLASHLGVVVPT"
     gene            3944..>4184
                     /locus_tag="VF362_04400"
     CDS             3944..>4184
                     /locus_tag="VF362_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX7590202.1"
                     /translation="MTDPASYRPAPGAIPTTPGVYRFRDPHGRVVYVGKAKNLRSRVS
                     SYFQGPAALHPRTRAMVTTASSIEWTVVRSEVEALV"
BASE COUNT          682 a         1420 c         1378 g          704 t
ORIGIN      
        1 cgccagaggg tcctgctctt cggtgtcgcc gtcgccctcg tcctgcgcgg gatcttcatc
       61 gttgcggggg ccgcggctct gtcgcacttc tcctgggtgt tctacctctt cgggttcttc
      121 ctcctgtgga ccgcgctgag cctcgcgcgc gagggtcacg aggacgagga ggacgtcgaa
      181 gccgagtatc acgagaaccg agcgatggtg atggtgcgtc ggctgcttcg ctccaccccg
      241 gagtatcacg gcggggcgct cgtcattcgg cgcgcgggtc ggcgcctcgt cacacccctc
      301 ctgctggtga tggtcgccat cgggaccacc gatgttctct ttgccctcga ctcaataccg
      361 gcgatttttg ggttgaccac cgacgcgtac atcgtcttcg ccgcgaacgc cttcgccctc
      421 atggggctgc gacagttgtt ctttgtcgtt cacgggctgc tgaccaggct cgtcttcctg
      481 tcgtacggac tggccgcgat cctggcgttc atcggcgtca aactggtctt cgaggcgctc
      541 cacgagaaca ccctgccgtt cattcacggc ggagagccgg tgacaggcct acccaccatc
      601 acgaccgaac tctcgctcgg cgtcatcatg gccgcccttg gcgtgtccgc gatcgccagc
      661 ctcatcgcca cgcggcgcag gaccgcgagt gcggccgcga cgggtcaggc ccgggactcc
      721 tccaccgact gaggccaggt cccgagggct ggccatgccc gcaccgtgtg ccgcgggaca
      781 agtcctgcga cgacgaaggg gtcgcccgcg acccaggcct caaccgcgtc gggagcgtcc
      841 gcgcggtaga taaacagcgc gcccggctcg agttcgtcgg cgtagcgccc gtaggcgaag
      901 agcgcaccct ggcgaacgag ggcatcgagg tactcgatgt gcgaactccg gatcgcgtcg
      961 cggtcttccg tgcggtcgtc gtaggcgtag tgaacggcga agaggtccat acgaacattg
     1021 aacacccgac acgccgagcg cgccccgggc tcgaacagat gttcgagtgg ttcgcggcac
     1081 gtacgatggt cccgtgaatg atcgtctcgt catccgcggc gcgcgggagc acaaccttcg
     1141 gtccatcgac ctggacctgc cgcgcgacgc gctgatcgtg ttcagcgggt tgagcgggtc
     1201 agggaagtcg tccctcgcct tcgacacgat cttcgccgag gggcagcgcc ggtacgtgga
     1261 atcgctgtcc gcgtacgcca ggcagttcct cggccagatg gacaagccgg acgtggactt
     1321 catcgagggg ctctctcccg cggtgagcat cgaccagaag tccacgaaca ggaacccgcg
     1381 ttcgaccgtc ggcacgatca ccgaggttta cgactacctc cggcttctgt ttgcccgcgt
     1441 cggcatccag cattgccccg agtgcgggga gcaggtgcgc gcccagacgg ccgagcagat
     1501 cgtcgactcg gttcttgccc tgcccgaggg gacgcgctac caggttctcg ctcccgtcgt
     1561 ccgggggcgc aagggcgaat accaggacct cttcgacgag ctcaagcagc aggggtttgc
     1621 ccgcgcgcgc gtcgacggcg aggccgtgga actgagcgcc cctcccacgc tcgagaagca
     1681 gatcaagcac accattgagg tgatcgtgga ccgcctcgtc gcgcgggagg gcgtgcgctc
     1741 gcggctcgcg gactcgatcg agacggccct gcgcgtcgcc gaggggctcg tcattatcga
     1801 tccggtggac ggagagatgg aggcgcgccg atactcggag aagcgtgcgt gccccaacga
     1861 tcacccgctg acgcttgaag agatcgagcc aaggacgttc tcgttcaacg ccccgtatgg
     1921 cgcgtgcccc gaatgccacg ggctcggcgt ccgccttgag gtggatcccg acctggtggt
     1981 tcccgacccg agcctgaccc tgcgcgcggg cgccctcgct ccgtgggccg cgacctcctc
     2041 cgagtacttc gcgcggaccc tcgccgccct cgcgtcaaaa ctcgaattct cgatggacgt
     2101 ccccttctcc gagctgtcgg acgaggcgaa gaaggccgtt ctcttcggaa gcgactactc
     2161 ggttcacgtt cgcttcaaga accgctacgg acgcgaacgc cagtacacga cggggtttga
     2221 gggcgtcgtc tcgtggatcg agcgcctgca ctcccagacg gaatcggagt ggtcgcgcga
     2281 gcgccacgag gcctacatgc gcgacgttcc ctgcgacgta tgcgagggca agcgcctcaa
     2341 gcccgaggtg ctctcggtcc tcgtcgctgg caagagcatc tccgacgtgt gcgagctgtc
     2401 gattcgcggg gcgcgcacct tcctcgcgga ggcggaactc gacgaacgct cacgccagat
     2461 cggggtgcag gttctcaagg agatcgacgc gcgactcgga ttcctcctcg acgtcgggct
     2521 cgactacctc agcctgtccc gcgcggcggg cacgctctct ggaggcgagg cgcagcggat
     2581 tcgcctcgcg acgcagatcg gttccgggct cgtgggggtg ctgtacgtcc tggacgagcc
     2641 cagcatcggg ctgcaccaac gcgacaacca ccgcctgatc gagaccctga cgcgcctgcg
     2701 cgacctcggc aacaccctca tcgtggtcga gcacgacgag gacacgattc gggccgcaga
     2761 ctggatcgtc gacatcgggc cgggggcggg ggagcacggc ggaagggtca ttcactccgg
     2821 aacgctcgag agcctcctga ccaacgagga atcgctgacg ggcgcgtacg tctcgggcag
     2881 gctcaggatc gacgtccccg cccagaggag ggagatcgat cccgggagga tgatcacggt
     2941 cgtcggcgcg agggagaaca accttcgcga catcgacgtg tcattcccca tcggcgtctt
     3001 cacgtccgtg acgggggtgt ccggatcggg gaagtccacc cttgtcaacg gcattctgta
     3061 cacggcgctc gccaacacgc tcaatggcgc gcggcaggtg ccgggccgcc acacccggat
     3121 cacgggcatc gaggatctcg acaagatcgt ccatgtggac cagggcccga tcgggcggac
     3181 cccccgctcc aatcccgcga cctacacggg ggtgtgggac aaggtccgca agctcttcgc
     3241 cgagaccgag gaggcgcgcc tgcgggggta cgggcccggc cgcttctcgt tcaacgtcaa
     3301 gggcggtcgc tgcgaatcct gttctggcga cggaaccctg aagatcgaga tgaacttcct
     3361 gcccgacgtg tacgtccgct gcgaggtctg ccacggttcg cgatacaacc gcgagacgct
     3421 ccaggttcac ttcaagggca agaccgtcgc cgacgtcttg aacatgccga tcgaggaggc
     3481 agcggacttc ttcggcgcga taccagcgat ctcgaggcag ctccgcaccc tcgtggacgt
     3541 ggggctcggc tacgtcagga tgggccagcc cgccccgacc ctgtcggggg gagaggccca
     3601 gcgcgtcaag ctcgcgtccg agcttcagcg acggtcgacg ggtcggaccg tctacgtcct
     3661 cgacgagccg accacgggcc tgcatttcga cgatgtccgg aagctcctcg cggtgctcca
     3721 gggcctcgtc gacaagggga ataccgtcat cgtaatcgag cacaacctcg acgtgatcaa
     3781 gtgctccgac tggatcgtcg accttggccc agagggcggc tccggcggcg ggatcgtggt
     3841 cgccgaggga acccccgagg acctggcgcg cgacgacgcg agccacaccg gacggttcct
     3901 ggcaagccac ctcggggtcg tcgtcccgac gtaggctggc gacgtgaccg acccggcctc
     3961 ataccgcccc gcgcctggtg caatcccgac tacccccggc gtctaccgat tccgagatcc
     4021 tcacgggcgc gtcgtctacg tcggcaaagc caagaacctt cgatctcggg tctcgtcgta
     4081 ttttcagggc cctgccgcgc ttcacccgcg cacgcgcgcg atggtgacca cggcgtcgtc
     4141 catcgagtgg acggtcgtcc gctccgaggt cgaggcgctc gtgc
//
LOCUS       DASVKP010000222         4447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049 k141_67655,
            whole genome shotgun sequence.
ACCESSION   DASVKP010000222 DASVKP010000000
VERSION     DASVKP010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67655"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VF362_04405"
     CDS             <1..197
                     /locus_tag="VF362_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590203.1"
                     /translation="ESEFDLAGGATRAGVGGVEGEAEGAEEGSEGVEGDADPEDLSVD
                     VLGDFAEVVLDFAAARESVR"
     gene            complement(212..352)
                     /locus_tag="VF362_04410"
                     /pseudo
     CDS             complement(212..352)
                     /locus_tag="VF362_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
     gene            complement(375..998)
                     /locus_tag="VF362_04415"
     CDS             complement(375..998)
                     /locus_tag="VF362_04415"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX7590204.1"
                     /translation="MAPTSPSSNLLEAEELKTALRERAREDRSQRSERLRAAAAQALA
                     AHTLALPEVQAASCVSVYASRPSEPGTLPLISALSEKGVRVLIPLLGDGLQRGWGEYL
                     GASDLTSRAPGRPPEPSSPFLPQVAIADADVVISPALAVDATGTRLGHGGGWYDRCLV
                     DARKDALLLVYCFPEELRTAEHPLPREAHDFPIHGAVTPDGVTLFDR"
     gene            1074..2012
                     /locus_tag="VF362_04420"
     CDS             1074..2012
                     /locus_tag="VF362_04420"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX7590205.1"
                     /translation="MALLGKAVIPAAGRGTRSLPASKAIPKEIIPLVDRPAIQYVVEE
                     AVAAGCDDIAIVTAPGKASLAAHFAPDPALEAALRSTGSAAQLEAVRHASALGPLTEI
                     LQDEPRGLGHAVACAEEFAAGEAFAVLLADDLLDERDPALATMMAIHARTGASVVLLM
                     DVPRDKAHLYGSVDPMRVDPAALPGEGGVPADAEIHRLTRLIEKPAPGEEYSTLAIIG
                     RYVFTPAIFDALRTIGKGSSGEIQLTEAINELAHRAPAEGGGVYGLVFRGRRYDTGDR
                     LEYLKATVQVAADRPDLGPDFLSWLRTYVKNRGDES"
     gene            2009..3244
                     /gene="glp"
                     /locus_tag="VF362_04425"
     CDS             2009..3244
                     /gene="glp"
                     /locus_tag="VF362_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX7590206.1"
                     /translation="MTRRTLAEHQRALLTGIAPIPAMEVLITDASGSILAEDLVSEGP
                     LPPVAIATCDGYALAAKDAPGATEGAPVSLAVAHDVGWDARGPRRHVSKTAARIVSGA
                     PMPVGADAVVPVGATDGGVAKVEVRATIRPGANVRAAGADAAAGKVLVRAGTRLGARQ
                     LGLAAGLGRRRLSVHPTPRVVVIPVGRELAEASRSGKGGTVPESNSHMLAVMAAEAGA
                     NAFRVGAVPDDRITLRATLEDQLVRADLIITTGGLSEARDETLPDVLRDLGEFDVYRV
                     ALMPGRRHGSGRISAAPGDAGTPVFALPGHPAAAIVAFEMYVRPVLRTMSGYAQKHRP
                     RVNVRSASAWESPAGVVQCVPVHVDRPHQEGASGKVVGDPSQPSLADLAVANALAVVP
                     EDVTSVAVGDILSCLLWDD"
     gene            3251..3844
                     /locus_tag="VF362_04430"
     CDS             3251..3844
                     /locus_tag="VF362_04430"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7590207.1"
                     /translation="MDSLFGRRMPVLACGHVVVRGLRPSDEGAWTDLLHRNRAWLRPW
                     EATLPGGRPLPAMPFRSFVRLDRSMWRKGRALAMAIEFDGALVGRIAITGIEWGSACN
                     GSMGYWIDEGHAGRGIVPQAAALLVRHGFSQGLHRVEIAARPENVSSLRVAKKLGFRD
                     EGLRRRYLYIDGDWRDHRVFALTSDERRLGDLWRCEG"
     gene            3897..>4447
                     /locus_tag="VF362_04435"
     CDS             3897..>4447
                     /locus_tag="VF362_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590208.1"
                     /translation="MPPVGLLAIAALGLILVYALPQRLRERADYAMVRTEDRFSADMR
                     VIRASVARMEARPVRPASPGRRAGSADATKKTTSALGESVMTRPAAPLDRAATRARRE
                     MVAMRRDRAKVLARRRAQSRRRGAIATLAAVASVGLWGSVAFASAPAWAAWVAVGTST
                     LFGASVVAGRRASRAYEEQDARL"
BASE COUNT          622 a         1513 c         1678 g          634 t
ORIGIN      
        1 aggagtcgga gttcgacttg gcgggcggcg cgacgcgcgc cggcgtcggg ggcgtcgagg
       61 gggaggcgga aggcgcggag gaagggtccg aaggcgtcga gggggacgcc gatccggagg
      121 acttgtccgt ggatgtgctc ggggacttcg ccgaggtcgt tctcgatttc gccgcggcgc
      181 gggagtccgt ccggtaaaag cccggaccct tgaaggcgac gccgacggca ccgatcacgc
      241 ggcgaagcga tcctccgcac tcggggcaca ccgaaagcgc gtcgtccgtg atcgggtgca
      301 ccgcctcaaa gcggtggccg cacacgtcgc aggcgtagtc gtaccggggc acgcgggcta
      361 tcctacgtcg ccgctcagcg gtcgaagagc gtgactccgt ccggcgtcac ggcgccgtgg
      421 atcgggaagt cgtgggcctc gcgggggagc gggtgctctg cggtgcgaag ctcctcgggg
      481 aagcagtaga ccaggaggag cgcgtccttg cgggcgtcga ccagacagcg gtcgtaccag
      541 cccccgccgt gacccagacg cgtgcccgtc gcgtcgacgg caagcgcggg ggagatgacg
      601 acgtcggcgt cagcgatggc gacttgcggg aggaaggggc tggagggttc ggggggccga
      661 cccggcgcgc gcgaggtgag gtccgaggcg ccgaggtact cgccccagcc gcgctgaagg
      721 ccgtcgccga gtagggggat caggacgcgc acgcccttct cgctgagcgc ggagatcagg
      781 ggcagcgtcc ccggctcgga cgggcgagac gcgtacacag agacgcacga ggcggcctga
      841 acctcgggaa gcgccagggt gtgagccgcg agcgcctggg cggcggccgc ccgaaggcgc
      901 tcgctccgct gcgaccggtc ttcccgcgcc cgttcgcgga gcgcggtctt gagttcttcg
      961 gcttccaaga ggttcgacga cggcgaggtg ggagccatac ggccattgaa tcaaccattg
     1021 gcgcgcggcg ccagggggtt ggaggtgtcg aggctcgcgg ttaggctcgg gacatggcac
     1081 tcctgggcaa ggcggtgatc cccgcggcgg gccgggggac gaggtccctg cctgcgagca
     1141 aggcgattcc caaagagatc atcccgcttg tcgatcggcc ggcgatccag tacgtcgtgg
     1201 aggaggccgt cgcggcggga tgcgatgaca tcgcgatcgt tactgcgccg gggaaggcct
     1261 cgctcgccgc ccatttcgcc ccggatcccg cgttggaggc ggcgctgagg tccactggaa
     1321 gcgccgccca gctcgaggcc gtcaggcacg cgtcggcgct tgggccgctg accgaaatcc
     1381 tccaggacga gccgcgcggc ctggggcatg cggtcgcgtg cgccgaggaa ttcgccgcag
     1441 gggaggcctt cgccgtgctc ctcgccgacg atctcctgga cgagcgagac cccgccctcg
     1501 cgacgatgat ggcgatccac gcgcggacgg gcgcgtctgt cgtcctcctg atggacgtcc
     1561 cgcgggacaa ggcgcacctc tacggctccg tcgacccgat gcgcgtcgac cccgccgcgc
     1621 tcccgggaga ggggggcgtt cccgcggatg ccgagatcca ccgcctgacg cgcctgatcg
     1681 agaagcccgc gcccggcgag gagtacagca ccctcgccat catcgggcgc tacgtcttca
     1741 ccccggcgat cttcgacgcc ctgcgcacga tcgggaaggg ctcctcgggc gagattcagc
     1801 tcaccgaagc gatcaacgag ctggcgcacc gcgcgcccgc ggagggcggg ggcgtctacg
     1861 ggctggtgtt tcggggacgc cggtacgaca cgggggacag gctcgaatac ctcaaggcca
     1921 ccgtccaggt cgccgcagac cgtccggacc tggggccgga cttcctctca tggctccgga
     1981 cgtacgtcaa gaatcgcggg gacgagtcat gacgcgcagg acccttgccg agcaccagcg
     2041 tgccctcctg acggggatcg cgccgatccc cgccatggag gtgctgatca ccgacgcctc
     2101 cggttcgatc ctcgccgagg acctcgtgtc cgagggtccg ttgccccccg tcgcgatcgc
     2161 gacgtgcgac ggatacgcgc ttgccgccaa ggatgccccc ggggcgacgg agggcgcgcc
     2221 cgtctcgctg gcggtcgcgc acgacgtcgg ctgggacgct cgaggcccgc gccgacacgt
     2281 gtcgaagacg gccgcgcgca tcgtctcggg ggcgccgatg cccgttggcg cggacgcggt
     2341 ggtcccggtc ggggcgacgg atggcggcgt cgcgaaggtg gaggttcgcg cgacgatccg
     2401 gccgggggcg aacgtacgcg cggcgggcgc ggacgcggcg gcgggcaagg tgctcgtgag
     2461 ggcggggacg cgcctgggtg cccggcaact cgggttggcc gcgggactcg gccgccgccg
     2521 gctgagcgtg cacccgacgc cgcgcgtcgt cgtcatcccg gtgggacgcg aactggcgga
     2581 ggcgagcagg agcggcaagg gcggcaccgt ccccgagtca aactcccaca tgctcgcggt
     2641 catggccgcc gaggcgggcg caaacgcatt cagggttggc gccgttccgg acgaccgcat
     2701 cacgctcagg gccacgctcg aggaccagtt ggtgcgggcc gacctcatca tcacgaccgg
     2761 cgggctgagc gaggcccgcg acgagaccct ccccgacgtg cttcgcgacc ttggcgagtt
     2821 cgacgtgtac agagtcgccc tcatgcccgg acgccggcac ggaagcggcc gcatctccgc
     2881 ggcgccaggg gacgcgggta cgccggtctt cgcgcttcct ggccaccctg cggcggccat
     2941 cgtggcgttc gagatgtacg ttcggcccgt ccttcgcacc atgtcgggct acgcgcagaa
     3001 gcaccgcccc cgcgtgaacg tcaggagcgc ctcggcgtgg gagtcgcccg cgggcgtcgt
     3061 gcaatgcgtt cccgtgcatg tcgataggcc ccatcaggag ggtgccagcg ggaaggtcgt
     3121 tggcgatcct tcgcaaccct cgctcgccga cctcgccgta gccaacgcgc tcgccgtcgt
     3181 ccccgaggac gtgacgtccg tcgcggtggg cgacatcctc tcgtgcctgc tgtgggacga
     3241 ctgagcgacc atggattcct tgtttggacg ccgcatgccc gtgctcgcct gcggccacgt
     3301 cgtcgtgcgg gggttgcggc cgtcggacga gggcgcgtgg accgatctgc ttcaccgcaa
     3361 tcgcgcgtgg ctgcgcccgt gggaggcgac gctccccggc ggtcggccgc tccccgcgat
     3421 gcccttccgg tcgtttgtgc gcctcgaccg aagcatgtgg cgcaaggggc gcgccctcgc
     3481 catggcgatc gagttcgacg gtgcactcgt cggcaggatc gcgatcacgg ggatcgaatg
     3541 gggctccgcc tgcaacggat cgatggggta ctggatcgac gagggccacg ccggacgcgg
     3601 gatcgtgccg caggcggcgg ccctgctcgt ccgccacggg ttttcccagg ggcttcaccg
     3661 cgtagagatc gcggcgcgac cagagaacgt gtcgagcctc agggttgcca agaagctggg
     3721 gttccgcgac gaggggctac gtcgccggta cctctacatc gacggagact ggcgggatca
     3781 cagggtcttc gcgctgacct cggacgagcg ccgactcggc gatctttggc gctgcgaggg
     3841 ctgacacgcc gcccgcgatg cgggccccgg cgccccggcg gccgtagcgt tcagatatgc
     3901 ccccggtcgg actcctcgct atcgcagcct tggggctcat cctcgtctat gcgctgcccc
     3961 agcggctgcg cgagcgggcc gactacgcga tggttcgcac cgaggatcgc ttcagcgccg
     4021 acatgcgcgt gatccgcgcg tcggtggcca ggatggaggc gcggcccgta cggcccgcgt
     4081 cgcccggccg ccgcgccggt tccgccgacg cgacgaagaa gaccacttcg gccttggggg
     4141 agagcgtcat gacccgtcca gcagccccgc ttgaccgagc cgcgacgcgc gcccgacgcg
     4201 aaatggtcgc gatgcgccga gaccgcgcca aggtgctggc gcgcaggcgg gcgcagtccc
     4261 gcaggcgcgg ggcgatcgcg acgctcgccg cggtcgcttc cgtgggccta tggggctctg
     4321 tcgcgttcgc ctctgcgccc gcgtgggcag catgggtggc cgtgggaact tcgacgctgt
     4381 tcggggcgtc cgttgttgcg ggccgccggg cctcccgcgc ctacgaggag caggacgcga
     4441 ggctcat
//
LOCUS       DASVKP010000223         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1142860, whole genome shotgun sequence.
ACCESSION   DASVKP010000223 DASVKP010000000
VERSION     DASVKP010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142860"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1205)
                     /locus_tag="VF362_04440"
     CDS             complement(51..1205)
                     /locus_tag="VF362_04440"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7590209.1"
                     /translation="MITWLRRPRVAVSITFLVNGLIIASWAPHVPYVRERLSLTEGVL
                     GVALLAGAVGSVSAMTLAAPLSARVGPHRLAMIAGVALALVAPLPVIAPNLWTLVIAL
                     LAWGAAIGSMDLAMNAVAADVEHRAARPLMSGFHGMFSLGALVGSIIAFGAIRAGLTP
                     LEHMVAVAALVLVGAWASFRALPPATRGMDEAPSGRLRWPGRALLVVGVIALIVLAAE
                     GAVENWSALVLKENFGSTASFAALGYGALSLTMAAFRFLGDGINKRVGRVALLAGGAG
                     LAGAGLAFGMIVGTPWAGVVGFGIMGIGLANTVPIVFSAGAAHGRTAAEGLSAVAVLG
                     YLGFLVAPPTIGFVAQGTSLPIALGGVAATLGVAALMSRAVAPAKATAAT"
     gene            complement(1267..1539)
                     /locus_tag="VF362_04445"
     CDS             complement(1267..1539)
                     /locus_tag="VF362_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590210.1"
                     /translation="MTATEGFEYRERKSGEVAIFNNGKLVKMMRGAEAKKFLQDVEKG
                     DAQQVMLDTVGDKGARRPSNGPIEGGKALGGDGQAHAHEVFRRRSS"
     gene            complement(1623..1844)
                     /locus_tag="VF362_04450"
     CDS             complement(1623..1844)
                     /locus_tag="VF362_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590211.1"
                     /translation="MPAPEGFAFEARKSGDVAIFHHGRLATTLRKEAAARFLTDAKTG
                     DPQELMARATGNYKRGNERKGVRGPAGPR"
BASE COUNT          299 a          725 c          670 g          247 t
ORIGIN      
        1 tagctggacg cacccgagtc gggccgcacc cgagtcgggc cgcaaccgga tcaggtcgcg
       61 gcggtcgcct ttgccggcgc cacggcgcgc gacatcagcg ccgcgacccc gagcgtcgcg
      121 gccaccccgc caagggcgat cgggagggat gtcccctgcg ccacgaagcc gatcgtcggc
      181 ggggcgacga ggaagcccag gtagccgaga accgcgacgg cgctcaggcc ctcggcggcc
      241 gtccgcccgt gcgcggcccc ggccgagaag acgatgggca ccgtgttcgc gagcccgatc
      301 cccatgatcc cgaaccccac cacaccggcc cacggcgtgc ccacgatcat cccgaaggca
      361 agccccgcgc ccgcgaggcc cgctcccccc gcgaggagcg ccacccgccc gacgcgcttg
      421 ttgatcccgt cgccaaggaa gcggaacgcc gccatcgtca acgagagcgc gccgtatccg
      481 agcgcggcga acgaggccgt cgaaccgaag ttctccttga ggacgagggc gctccagttc
      541 tcgacggcgc cctcggccgc gaggacgatc agggcgatca cgccgacgac gaggagagcg
      601 cgaccgggcc agcggagcct gcccgacggg gcctcgtcca tgccgcgcgt cgcgggaggc
      661 agagcccgga agctcgccca cgcgcccacg agcaccaagg cggccacggc aaccatgtgt
      721 tcgaggggag tcagccccgc gcggatcgcg ccaaatgcga tgatcgagcc gacgagcgcc
      781 ccgaggctga acatgccgtg gaagccggac atgagcgggc gcgccgctcg gtgctccacg
      841 tccgccgcca ccgcgttcat cgcaaggtcc atcgaaccga tcgcggcgcc ccacgcgagg
      901 agggcgatca cgagggtcca gaggttcggc gcgatgaccg gcagcggcgc cacaagcgcg
      961 agcgcgaccc cggcaatcat cgcgaggcgg tgcgggccca cccgcgcgct cagcggggcc
     1021 gcgagggtca tggccgatac ggagccgacg gcgcccgcga ggagggcgac gcccaggacc
     1081 ccctcggtga gcgaaagccg ttcgcgcacg tagggaacgt gcggcgccca cgacgcgatg
     1141 atgaggccat tgaccaggaa ggtgatcgag accgcgacgc gcggccgacg aagccaggtg
     1201 atcatgcaga accttcctcg ggggcccgac ggcgagacca cccgctcccg ccagcccctc
     1261 ggcgcgctag ctggagcgcc gccggaagac ctcgtgggcg tgcgcctggc cgtcgccgcc
     1321 gagggccttg ccaccctcga tcggcccgtt gctcgggcgt cgcgcgccct tgtcccccac
     1381 ggtgtcgagc atgacctgct gggcgtcgcc cttctccacg tcctggagga acttcttcgc
     1441 ctcggcgccg cgcatcatct tcacgagctt gccgttgttg aagatcgcga cctcgccgga
     1501 cttgcgctcg cggtactcaa atccttctgt tgccgtcatg gctccatcgt gtcacgggtg
     1561 gcgggccgcc gtctccaggt cttcgctgga ccggcggcgt cggcaacaag cgtcgcccga
     1621 ggctagcgag gccccgcagg gcctcggacg cccttgcgtt cgttgccgcg cttgtagttg
     1681 ccggtggcgc gggccatcaa ctcctggggg tcgccggtct tcgcgtccgt gaggaaccga
     1741 gcggccgcct ccttgcggag cgtggtcgcc aggcggccgt ggtggaagat cgccacgtcc
     1801 ccgctcttgc gggcctcgaa cgcgaatccc tcgggtgctg gcatggctcc atcgtcccac
     1861 tgccgcctcg acctgccagt cgccagccca aaaggtcgcg gaaagacggc ggactacctg
     1921 cgaccctttg cgcgttgccc t
//
LOCUS       DASVKP010000224         3043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_564368, whole genome shotgun sequence.
ACCESSION   DASVKP010000224 DASVKP010000000
VERSION     DASVKP010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564368"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VF362_04455"
     CDS             complement(<1..393)
                     /locus_tag="VF362_04455"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX7590212.1"
                     /translation="MLETLVCSRHRALVAYAYLLTRDVGDAEDLVQDALVAAFGGRAT
                     FPSIGHAEAYVRRAIATKFVDGARRRTRERARVDRVKIAHYADGPESGVVGRVDVASA
                     LAELAPRVRACVVLRYLADQSTGETAHAL"
     gene            complement(478..1746)
                     /locus_tag="VF362_04460"
     CDS             complement(478..1746)
                     /locus_tag="VF362_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590213.1"
                     /translation="MAKLGGRVWALIAGAGALLALCAAAWFAGWWPFGGSGSEPTPTP
                     SVSASPSASPRPSFVERQVTVAPNVPVSELLTAAVLDSAGNGWVVAVYDATSRDDTGV
                     ESPGPKALYLISPEGVRYEVGNLDTLGMSAPDLVAWDYARRKVLIVDNLMDMKVVDMM
                     TGAIDGSWLFCDHEGYVRGSARDGAWLLRGSCNGEGIDGLYGDAGVEVPSSIVGKGFG
                     LTVFDVGDVQVQSEFETAPDVRFTAFYADGTQATIPSSMAGDCYLLGKGKGDTFAAYC
                     YTSAGAVDVWEFPVDGSTPVDVVTSAQLEAFRLELGVPNPADFFVSGYCSAADLPVVE
                     VTWDQSRLGVLKAGSLAAAAQPPHPFRHCLAQSGTSALVSGDGLLWLTDFATGSTVEL
                     LPGSGASAAARVVGAAHVVGTEGYRALQQP"
     gene            1811..2752
                     /locus_tag="VF362_04465"
     CDS             1811..2752
                     /locus_tag="VF362_04465"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014922627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEX7590214.1"
                     /translation="MDVSDVPVQVSQTFDPARWRAIDGFPTGDITYHRWVERTGREGE
                     RDLPAVRIAFNRPELRNAFRPQTVDELYRALDHARQTPDVAAVILTGNGPSPKDGVWA
                     FCSGGDQSVRGKDGYLYEGSAADAPAHGGRLHILEVQRLMRTSPKVIIAAVNGWAAGG
                     GHSLHVVADLSIASREHARFKQTDANVGSFDAGYGSALLARQVGDKRAREIFFLAREY
                     SAEDAERWGAVNEVVDHDRLEEVALEYAALVAAKSPQAIRMLKFAFNLADDGLAGQQV
                     FAGEATRLAYMTEEAQEGRDAFLERREPDWTRFPYAY"
     gene            complement(2759..>3043)
                     /locus_tag="VF362_04470"
     CDS             complement(2759..>3043)
                     /locus_tag="VF362_04470"
                     /inference="COORDINATES: protein motif:HMM:NF037564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HEX7590215.1"
                     /translation="ILVLRATVRYKISQDPETMLAGTVNGTGYIAAGGKYLEIRWSKA
                     DRRDTFHLTTVDGGPVYLQPGNTWVELLPEAGSRESQVLKFDDRVITTRP"
BASE COUNT          490 a         1089 c         1026 g          438 t
ORIGIN      
        1 gagggcgtgg gcggtctccc ccgtcgactg gtcggccagg tacctcagca ccacgcaggc
       61 ccggacgcgc ggggcgagct cggcgagcgc cgaggcgacg tccacccgcc cgacgacacc
      121 cgactccggg ccgtcggcgt aatgggcgat cttgacccga tcgacgcgcg ccctctcccg
      181 cgtgcgtcgc cgcgccccat ccacaaactt cgtcgcgatc gcccgtcgca cgtacgcctc
      241 ggcatgcccg atgctcggga acgtggcccg gccaccgaag gccgccacga gtgcatcctg
      301 caccagatcc tcggcgtcac ccacgtcccg cgtcaggagg taggcgtacg cgacgagagc
      361 gcgatggcgt gagcaaacca gggtttcgag catgggctcc caccttggca tggccactcc
      421 ttgtcgccta tgaagcatca acgacgccga ggggggaacg gttgggggta ttccccccta
      481 cggctgctgg agggcccggt agccctcggt cccaacgaca tgcgcggctc ctacgacgcg
      541 cgcggcagcg ctggccccgg atccgggaag caactcaacc gtggatccgg tcgcgaagtc
      601 ggtgagccag aggagcccgt caccgctcac gagggccgac gtccccgact gtgcgaggca
      661 gtgacggaag ggatgcgggg gctgggctgc ggcggcgagc gacccggcct tgagaacgcc
      721 gaggcggctc tggtcccagg tcacctccac cacgggcagg tcggcggcgg agcagtagcc
      781 cgacacgaag aagtccgcgg gattcggaac gccgagctca aggcggaatg cctcgagctg
      841 cgcggacgtg acgacgtcca cgggcgtcga gccgtcgaca gggaattccc agacgtcgac
      901 ggcccctgcg ctcgtgtagc agtacgcggc gaaggtgtcc cccttcccct tgcccaacag
      961 atagcagtcc cccgccatcg aggaggggat ggtcgcctgg gttccatccg cgtagaacgc
     1021 cgtgaatcgc acgtccggcg ccgtctcgaa ttcgctctgg acctggacgt cgcccacgtc
     1081 gaagacggtg aggccgaagc cctttccgac gatggaactc gggacctcga cgccggcgtc
     1141 gccatagagg ccatcgatgc cctcgccatt gcaactgccg cgcaggagcc acgcgccgtc
     1201 tcgcgccgag ccccgaacgt aaccctcgtg atcgcagaac agccacgagc cgtcgatcgc
     1261 tcccgtcatc atgtcgacga ccttcatatc catgaggttg tccacgatca ggaccttgcg
     1321 gcgcgcgtag tcccacgcga ccaggtcggg ggcggacatg cccagggtgt cgagatttcc
     1381 gacctcgtac cggacgccct cgggcgaaat caggtagagg gccttcggtc cgggcgactc
     1441 gacccccgtg tcatcgcggg acgtcgcgtc gtagaccgcg accacccacc cgttacccgc
     1501 ggagtcgagg acggccgcgg tcagcagttc gctcacggga acgttggggg cgacggtcac
     1561 ttggcgctcg acgaacgaag gccgcgggga ggcgctgggg ctcgccgaca ccgacggcgt
     1621 cggggtcggc tcggaacccg agccgccgaa cggccaccag cccgcgaacc acgcagccgc
     1681 gcagagcgcc aggagcgcgc cggcgccagc gatcaacgcc cagacccttc ctcccagctt
     1741 ggccatgccc actcctccgt cggccgagcc ccggcacgcc ctggcaacgc gcttctcgaa
     1801 ggctaggctc atggacgtga gcgacgttcc cgtccaggtc tcccagacct tcgatcccgc
     1861 gcggtggcgc gccatcgacg gcttccctac tggtgacatt acctaccatc gctgggttga
     1921 gcgcacaggg cgcgagggcg agcgggacct tcccgccgtg cggatcgcct tcaaccgccc
     1981 cgaactgcgc aacgccttcc gcccccagac cgtcgacgag ttgtatcgcg cgctcgatca
     2041 cgcgcgccag acgccggacg tcgcggccgt catcctcacg gggaacgggc cgtcccccaa
     2101 ggacggggtg tgggcgttct gctccggtgg cgaccagagc gtgcgcggca aggacggcta
     2161 cctctacgag ggctcggcgg cggacgcccc cgcccacggc ggccgactgc acatcctcga
     2221 ggtccagcgg ctcatgcgca cctcccccaa ggtcatcatc gccgccgtga atggctgggc
     2281 ggcgggcggc gggcacagcc tccacgtcgt ggccgacctc tccatcgcga gccgcgagca
     2341 cgcccgcttc aagcagaccg acgcgaacgt cgggtcgttc gacgcgggct acgggagcgc
     2401 gctactcgcc cgccaggtcg gcgacaagcg cgcccgggag atcttcttcc ttgcgcggga
     2461 gtactcggcg gaggatgccg agcggtgggg cgccgtgaac gaggtcgtcg accatgatcg
     2521 gctcgaggag gtcgcgctcg agtacgcggc gctcgtggcc gccaagtcgc cccaagccat
     2581 ccggatgctc aagttcgcct tcaacctcgc ggacgacggc ctcgcgggcc agcaggtgtt
     2641 cgcgggcgag gcgacgcgcc tcgcctatat gacggaggag gcgcaggagg gacgcgacgc
     2701 gttcctggag cggcgcgagc ccgactggac ccggttccca tacgcgtact aggccgagct
     2761 acggccttgt ggtgatcacc ctgtcgtcga acttcaacac ctgcgactcg cgcgaccccg
     2821 cctcggggag aagctcaacc caggtgtttc ccggctggag gtagaccggt ccgccgtcga
     2881 ccgtggtgag gtggaacgtg tcacgtcggt cggccttgga ccaccggatc tcgaggtact
     2941 ttccgcccgc ggcgatgtat ccggttccgt tgaccgtccc cgcgagcatg gtctccgggt
     3001 cctggctgat cttgtagcgc accgttgcgc ggagcaccag gat
//
LOCUS       DASVKP010000225         6624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_234924, whole genome shotgun sequence.
ACCESSION   DASVKP010000225 DASVKP010000000
VERSION     DASVKP010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234924"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VF362_04475"
     CDS             complement(<1..937)
                     /locus_tag="VF362_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX7590216.1"
                     /translation="MAIEDAAIEGPAIEDAELGTSLRRNTAIMAAGTATSRALGLVRT
                     ALLAAAIGVNAGAASSFDVANKLPGVLLTLLASGVLNAVLVPQVVRAMQRRGGKQTVD
                     RIVTLGIVAMLAITALLTAAAPLFILAYTSKFTPELTALTVAFALWTIPQLFFYGVYT
                     LFGQILNARSRFGPFMWAPAANNLVAIVGLVVFLMLFGPYAVPSAGGDAYGLAGDWSP
                     GKIALLAGVATLGIAVQALVLIVPLARIGYRWRWQWSGPKGELDGIKRVAGWALAAVL
                     LEQVGIAFVTRVASGALSAGGGAPDVASNAALTNAL"
     gene            complement(939..2849)
                     /locus_tag="VF362_04480"
     CDS             complement(939..2849)
                     /locus_tag="VF362_04480"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HEX7590217.1"
                     /translation="MTAGRRLGQIAGALAAAVLLAGASAGPIRAAESPATGDLSADVT
                     SYGSPILGPDSSLTVGVTIANDAGSDADNVEVIVGVTTDPLADRASLASWQSGESAPP
                     TREVARRLALGTGVVAAQTTVTTTVVAPPSALGLGANPWAVYGVTVAVHASGKDVKEF
                     RTTATYLDTTPSVMPLAIIATAAGAPERVSAVLSAAAAARVSLLVDPTALALAAGAVV
                     STPTDVYSLPAGHVDVASLARALDSGILPTALDASAKADAPGADRPWIAVLPSLDRES
                     LVLAQHLGAAAILLQPGTTPPTATNDEGLEGVAPALVDAGEGSPLIVVADPGLSLALA
                     GAHVTAALRPATAVAESALLAMANRDRQIVVASPGTSWMLDAEHRSPALEALAACPWN
                     RMVSLTSVLDGGTPVPTVLPEAPSTDGDITVSQVSVAANDLRDLGYLAAATSTPAAVY
                     SAPAAVLLSALSFEGRGDPAARADTIARALAIAQDVLDRVSLPRGSDLNLISTSGSVP
                     ITVTNELAVDVTVTVVLETRSPNLRPTGQATVTLKPNTSETVLIPVRAVSSANVAAFV
                     HLTDTEGHRLSSDTIVRVRVRADWGTAFTRILGAVALLLLIGGIWRTARHGRRDTRGT
                     PGDEPLPFGSDG"
     gene            complement(2846..3397)
                     /locus_tag="VF362_04485"
     CDS             complement(2846..3397)
                     /locus_tag="VF362_04485"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7590218.1"
                     /translation="MLSSEPPDSHAKPKPPVGDAKPPARRQLRGKGSLLPVSNETSAG
                     GIAIFVEDGAARAAVIGRRNRQGKLEWCLPKGHLEEGETPEEAAIREVAEEAGVTSEI
                     ILALGIIDYWFTGEDRRIHKVVHHYLLETTSDEITTENDPDHEAEVAEWVSLRDLPDR
                     LSYPNERRMAEVAVDLLALEGDL"
     gene            3459..4913
                     /locus_tag="VF362_04490"
     CDS             3459..4913
                     /locus_tag="VF362_04490"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012880101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX7590219.1"
                     /translation="MVVATGFPPLDVLRQRAVGLWTALPADVAQLASAFAGAGHELAL
                     VGGPVRDAFLGRSSADLDFATSALPDDTEALLRSWGANTWDMGREFGTIGGRRGDTLV
                     EVTTYRADSYGDQSRKPEVAFGDSLESDLARRDFTINAMAVRLPSLEFVDPFDGLADL
                     AAGVLRTPIEPEISFSDDPLRMMRAARFASQLGLEVEATTLGAIERMSERLGIVSAER
                     VRDELTKLLLGADPRRGLEILVDCGLADQVIPEVPALRLEVDEHHHHKDVYQHTLTVI
                     DQAIALETGPEGAVPGPDLVLRLAALMHDIGKPATRKYEAGGVVSFHHHEIVGAKMTA
                     RRLKALRFDKAIILAVVRLTELHLRFHGYGEASWTDSAVRRYVTDAGEQLERLHRLTR
                     ADCTTRNRRKADQLSFAYDDLEHRIAELREHEELKAIRPDLDGEQIMAILGIPPGRAV
                     GQAYAFLLELRMEEGPLGEDEAQKRLLAWWSARA"
     gene            complement(4910..6205)
                     /locus_tag="VF362_04495"
     CDS             complement(4910..6205)
                     /locus_tag="VF362_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7590220.1"
                     /translation="MSVIRDLRHLMRGAGFRRLAATRLISQLGDGMFQAGLASLLFFD
                     PTKQATAGSIALGFFLLLAPFTLVGPFVGPLIDRWQRQRIILVGNLVRVGLAAFLGYL
                     MITHGPVGVLYVAAVLTLSINRFLLAAMAAAIPRVVADEDLLAANAILPTLGTIAAAL
                     GAGIGVVVEFLAPGLGNTQSALAALLASGAVFGLSSWTATALGRRELGPVATLDGTKV
                     REQMSGLLRELRDGARYLARRVTPAQAILVMAAQRLLYGLMFVASILISRQLLSGAGD
                     AQGGLGAFGLVLGFAAVGFGLAAIVTPALGHLVPRHRWVVWCLVVGAGGQALLAASHS
                     RWALLSAAVIVSFAVQGGKIAVDTIVQRDTEDAVRGRAFSLYDVAYNVAFFSSAGIAV
                     LVLPDSGYSRTVMAGMVVAYLVAAALYALAPKEPRALVR"
     gene            6302..>6624
                     /locus_tag="VF362_04500"
     CDS             6302..>6624
                     /locus_tag="VF362_04500"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEX7590221.1"
                     /translation="MRFGEAFTTCMRKYADFSGRARRSEYWWFYLALQLIMLPFAVAF
                     TVADVMLFVGADYQPDGNMSLSGADVAPLIVTFALMMIVSLAFTLPSLAAQARRLHDM
                     GQTGL"
BASE COUNT         1080 a         2207 c         2404 g          933 t
ORIGIN      
        1 tgagggcgtt ggtgagcgcg gcgttgctcg cgacgtcggg ggcaccgccg ccggcggaca
       61 acgctccgct cgcgacgcgc gttacgaacg cgatgcccac ctgttccaat aggacagcgg
      121 cgagcgccca gccggcgacc cgcttgatcc cgtcgagctc acccttcggc ccggaccact
      181 gccaccgcca gcggtagccg atgcgcgcga gcgggacgat cagcacgagc gcctggaccg
      241 cgatcccgag cgtcgccacc ccggcaagca gggcgatctt cccggggctc cagtcgcccg
      301 cgagcccgta ggcgtcgcct cccgcagagg ggaccgcgta tggcccgaag agcatcagga
      361 agacgacgag ccccacgatg gccacgaggt tgttggccgc gggcgcccac atgaacggac
      421 cgaagcgcga gcgcgcattg aggatctggc cgaagagcgt gtagacgccg tagaagaaca
      481 gttgcggaat cgtccagagc gcgaacgcga cggtgagggc cgtcagttcc ggcgtgaact
      541 tggacgtgta ggcgaggatg aagagcggcg cggcggcggt gaggagcgcg gtgatcgcca
      601 gcatcgcgac aataccgagg gtgacgatcc gatcgaccgt ctgcttgccg cccctgcgct
      661 gcatcgcgcg gacgacctgc ggtacgagaa ccgcgttgag cacgccggag gccagcagcg
      721 tcaggagcac gccgggaagc ttgtttgcga cgtcaaacga actcgccgcg ccggcattga
      781 cgccgatcgc ggccgcgaga agcgcggtcc gcacgaggcc caacgcgcgc gacgtcgcgg
      841 tgcccgccgc catgatcgcc gtgtttcgac gcagggacgt gcccagttcc gcgtcttcga
      901 tcgctgggcc ttcgattgca gcgtcttcga tcgccatgtc agccatcgct cccgaacggc
      961 agaggttcgt cgccgggggt cccgcgcgta tcccggcgcc cgtgccgcgc cgtccgccag
     1021 atgccgccga tcaggagcag aagcgcaacc gcgcccagaa tgcgcgtgaa ggccgtgccc
     1081 cagtcggcgc ggaccctgac ccgcacgatc gtgtcggaag agaggcggtg accctcggta
     1141 tcggtgaggt ggacgaacgc ggcgacattg gcgcttgaga ccgccctgac ggggatcagg
     1201 accgtctctg acgtgttcgg tttgagggtc accgtcgcct gccccgtggg tcggagattc
     1261 ggcgaccggg tttccagcac caccgtgacc gtgacgtcaa ccgcgagttc gttcgtcacg
     1321 gtgatgggga cgctccctga ggtggagatg aggttgaggt cgctgccgcg gggaagggac
     1381 acccggtcca ggacgtcctg ggcgatcgcg agagcgcggg cgatcgtgtc cgcccgcgcg
     1441 gcggggtctc cgcgcccctc gaaggacagc gcggaaagga gcacggccgc cggtgcggag
     1501 tagacggcgg cgggggttga cgtagcggcc gcgaggtacc cgaggtcgcg caggtcgttc
     1561 gccgcgacgc tgacctggct caccgtgatg tcgccgtccg tcgacggggc ctcggggagg
     1621 acggtcggga cgggggttcc cccgtcgagg accgaggtga gcgaaaccat ccggttccag
     1681 ggacacgccg cgagcgcctc aagcgccggc gagcggtgtt cggcgtcgag catccaggac
     1741 gtccccggcg aggcgaccac gatctgtctg tcccggttgg ccatggcgag gagagcggat
     1801 tccgcaacgg ccgtagccgg acggagggcc gccgtcacgt gggcgcccgc cagggcgagg
     1861 gaaagccccg gatcagcgac cacgatgagg ggagatccct cgcccgcgtc gacaagtgcg
     1921 ggtgccaccc cctcaagacc ctcgtcgttc gtcgccgtgg ggggcgttgt tcctggctgg
     1981 aggaggatcg cggccgcgcc aaggtgctgg gcgagcacca gggactcgcg gtcgagcgag
     2041 ggaaggacgg cgatccaagg acggtccgcc ccaggcgcgt ccgccttcgc ggaggcgtcg
     2101 agcgcggtgg ggagtatccc agagtcgagg gcgcgcgcga gggaggcgac atccacatga
     2161 ccggcgggaa gcgagtagac atcggtggga gtggaaacga ctgcgcccgc agcgagggcg
     2221 agcgccgtcg gatcgaccag gagcgacacg cgggccgccg cggccgcgct gaggaccgcg
     2281 gagactcgct ccggcgcgcc cgcggccgtc gcgatgatgg cgaggggcat gaccgaaggc
     2341 gtcgtgtcga ggtaggtcgc cgtagtccgg aactccttga catccttccc gctggcgtgg
     2401 accgccaccg tgacgccata gaccgcccag ggattggcgc ccaatcccag cgcggagggc
     2461 ggggcgacga cggtcgtcgt caccgtcgtc tgcgcggcaa cgactccggt gcccagtgcc
     2521 aggcggcgcg ccacttcgcg ggtcggcggc gccgactcgc ccgattgcca actggccagc
     2581 gaggctcgat ccgcgagagg gtccgtggtc acgccgacga tcacctccac gttgtcggcg
     2641 tcggatcccg cgtcgttggc gatggttacc ccgaccgtga gggacgaatc gggccccagt
     2701 atcggggaac cgtaactcgt gacatccgcg ctcaggtcgc cggtcgccgg ggattccgcc
     2761 gcgcggatgg ggcccgcgga tgctcccgcg aggagcaccg ctgccgcgag ggcgccagca
     2821 atctgcccga ggcgccgacc ggcggtcaca ggtccccctc gagcgcgagg agatccacgg
     2881 caacctcggc catccggcgc tcattgggat aggacagccg gtcgggaagg tcccgcagcg
     2941 acacccactc ggcgacctcg gcctcgtgat cggggtcgtt ctcggtggtg atctcgtcgc
     3001 tggtcgtctc caggagatag tggtgaacca ccttgtggat ccggcggtcc tccccggtga
     3061 accagtagtc gatgatcccg agagccagga tgatttcgga ggtcacgccc gcctcttccg
     3121 cgacctcgcg aatcgcggcc tcctcggggg tctcgccctc ctcgagatgg cccttgggaa
     3181 ggcaccactc gagcttccct tggcgattgc ggcgtccgat gaccgcggcg cgcgccgcgc
     3241 cgtcctccac gaagatggcg atcccgccgg cggaggtctc gttcgacacc ggaaggaggc
     3301 ttcccttgcc gcgcaactgt cgcctcgcgg gcggcttcgc gtcgccgacg ggcggcttcg
     3361 gcttcgcgtg agagtcgggt ggttcggacg aaagcatgac tcactctaac cagcggggcg
     3421 cgccgggcgg agtcgtgacc cgctctctgg gatgcttggt ggttgtggcc accggattcc
     3481 cccccctcga cgtgctccgt cagcgagctg tggggctctg gaccgcactg ccagcggacg
     3541 tcgcccaact cgcctccgcc ttcgcgggcg cggggcacga actcgcgctg gtggggggcc
     3601 cggtgaggga cgcgttcctc ggtcgctcga gtgccgacct ggacttcgcg acgtcagcgc
     3661 tgccggacga tacggaggcg ctgctccgtt cctggggtgc gaacacctgg gacatgggcc
     3721 gcgagttcgg aaccatcggg ggccgaaggg gagacacgct cgtcgaggtc acgacgtatc
     3781 gcgccgactc ctacggggac cagtcgcgca agccggaggt cgccttcggg gacagcctcg
     3841 agagcgacct cgcgcgccgc gacttcacca tcaacgcgat ggccgttcgg ttgccgtccc
     3901 tcgagttcgt cgatccgttc gacggcctcg cggatcttgc cgccggagtc ctacggacgc
     3961 cgatcgagcc cgagatctcc ttctccgacg acccgcttag gatgatgcgc gccgcgcgct
     4021 tcgcgtctca gttgggactt gaggtcgagg caacgacgct cggcgccatc gagcgaatgt
     4081 cggaaaggct tgggatcgtc tcggcggagc gggtcaggga cgagctgacc aagctcctgc
     4141 tcggcgctga cccccgtcgc ggcctcgaga ttctcgtgga ctgtgggctc gccgaccagg
     4201 tgattcccga ggttccggcg ctccgcctcg aggtggacga gcaccatcac cacaaggacg
     4261 tctaccagca cacccttacc gtgatcgatc aggcgatcgc gctcgagacc gggccggagg
     4321 gcgccgttcc cggccccgac ctcgtgctgc gtcttgcggc cctgatgcac gacatcggca
     4381 agcccgcgac ccgaaagtat gaggcgggcg gcgtcgtgag cttccaccac cacgagatcg
     4441 tgggcgccaa gatgacggcg aggcgcctca aggcccttcg gttcgacaag gcgatcatcc
     4501 tggccgtcgt gcgtctcacg gaactccacc tgcggttcca cgggtatggc gaggcctcgt
     4561 ggaccgattc cgccgtccgg cgctacgtga ccgacgcggg ggagcaactc gagcgcctcc
     4621 accggctgac gcgcgcggac tgtacgacgc ggaaccgccg caaggcggat cagttgtcct
     4681 tcgcgtacga cgacctggag caccgcatcg cggaactgag ggagcacgag gaactcaagg
     4741 ccattcgccc ggatctcgac ggcgagcaga ttatggcgat cctcgggatc ccgccgggcc
     4801 gcgcggtggg acaggcgtac gcgttcctcc tggagttgcg gatggaggag ggcccgctcg
     4861 gcgaagacga ggcgcaaaag cgactgctcg cctggtggtc cgcccgggcc tagcggacga
     4921 gcgcccgcgg ctccttcggc gcgagcgcgt acaacgcggc ggcgacgagg taagcgacga
     4981 ccatgccggc catgaccgta cgggagtagc cggagtcggg caggacgagc acggcgattc
     5041 cggcggagga gaagaaggcg acgttgtagg cgacgtcgta gagcgagaag gcgcgacccc
     5101 tgacggcgtc ctccgtgtcg cgctgcacga tcgtgtcgac ggcgatcttg cccccctgga
     5161 cggcgaagct gacgatcacg gccgccgaga ggagggccca tcgcgagtgc gaggccgcca
     5221 gaagcgcctg cccgcccgct cccacgacga ggcaccacac cacccaacgg tggcggggaa
     5281 cgaggtgccc gagcgcgggg gtgacgatcg ccgcgaggcc gaacccgacg gcagcgaaac
     5341 ccaggacgag tccgaacgcc cctagtcctc cctgcgcgtc gccggctccc gagagcagtt
     5401 gccgcgagat gaggatcgac gcgacgaaca tcagcccata gagcaggcgc tgggcggcca
     5461 taacgaggat ggcttgagcc ggggtaacgc gccgggcgag gtagcgggcg ccgtccctca
     5521 actcgcggag caggcccgac atctgctccc tgacctttgt gccgtcgagg gtagcgaccg
     5581 gcccgagctc gcggcgcccg agggccgtcg ccgtccacga cgagagcccg aacacggcgc
     5641 ccgatgcgag cagggccgcc aacgcgctct gggtattgcc gaggcccggc gcgaggaatt
     5701 cgaccaccac cccgattccc gcgccaagcg cggcggcgat ggtcccgagg gtggggagga
     5761 tggcgttcgc tgccagaagg tcctcgtcgg cgacgacccg aggtatcgcg gcggccatcg
     5821 cggccaggag aaagcggttg atcgacaggg tgaggacggc cgccacgtac aggactccca
     5881 cgggcccatg ggtgatcatc aggtacccga ggaaggcggc gaggccgacg cgcacgaggt
     5941 tgcccaccag gatgatgcgc tgccgctgcc agcgatcgat gagggggccc acgaaggggc
     6001 cgacaagcgt gaagggggcc agaaggagga agaagccgag ggcgatcgat cccgcggtcg
     6061 cctgcttcgt cgggtcgaag aacaggaggg atgcgagtcc ggcctggaac atcccgtcgc
     6121 cgagttgcga gatgaggcgg gtggccgcaa gcctccggaa gccagcaccc ctcatgaggt
     6181 ggcggaggtc gcggatcacg gacatgcgac aaaggtaccg gtcacgagcg cgcggatcgc
     6241 cgacgtcgta cgctaggcca cgacgcggcc caccgggtgg ccgtgaggaa acggggctgc
     6301 gatgaggttc ggcgaggcat tcacgacctg catgaggaag tacgcggact tcagcggccg
     6361 cgcgcggcgg tcggagtact ggtggttcta cctggccctc cagctcatca tgctgccgtt
     6421 tgcggtggcg ttcacggtcg cggacgtcat gctgttcgtc ggtgcggact accagcccga
     6481 cggcaacatg tcgctctcgg gagcggacgt cgcacccttg atcgtgacct tcgcgctgat
     6541 gatgatcgtg tcgctcgcgt tcaccctccc gagcctcgcc gcccaggccc gccgcctcca
     6601 cgacatgggg cagaccggcc tatg
//
LOCUS       DASVKP010000226         1826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_815274, whole genome shotgun sequence.
ACCESSION   DASVKP010000226 DASVKP010000000
VERSION     DASVKP010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815274"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VF362_04505"
     CDS             complement(<1..1095)
                     /locus_tag="VF362_04505"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX7590222.1"
                     /translation="MDALTKLADDYYAYATSDSPLRLMWNGKLDHLAEWDDFSPAAVG
                     RRKARYLAFAEAADALQIGDDPRDIALRDSISTGARSAAIRLTWEAELLQVSPKMGLF
                     EMCLSFIGTYPLKTAEHGEQYLEKLRRMPGALRVLADAARAAAAEGRVATARQLGATA
                     DSIDAYLASPPGPGERLCCQEAPTKLGADEAGAWREARDAIIATTVREGLVAYGAALR
                     DLARDARPDEKPGLCNLPGGLEVYRDAMWANLLTDRTAQEVHQIGLDKVAELEDEYRA
                     IAGPLFGLAEVSDIYARLRDDKALVYTSPEAIVADAEAALARAGVAAATWFGTMPASK
                     CPAHATDDGAMAYYSTPDLEIGKEAAFYFNT"
     gene            1354..>1826
                     /locus_tag="VF362_04510"
     CDS             1354..>1826
                     /locus_tag="VF362_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7590223.1"
                     /translation="MTEASADKYPLHRSHLDGADGEVAASPRFPAIERDVLAYWESDG
                     TFQASIDNRAGTPEHPAEEFVFYDGPPFANGLPHYGHLLTGYAKDVVPRFETMRGKLV
                     ERRFGWDTHGLPAELEAERILGITDKAQIEEMGIGAFNDACRESVLKYTKEWEEYV"
BASE COUNT          265 a          640 c          655 g          266 t
ORIGIN      
        1 cgtgttgaag tagaaggcgg cctccttgcc gatctcgagg tcgggcgtcg agtaataggc
       61 catcgcgccg tcgtccgtcg cgtgggccgg gcacttcgac gcgggcatgg tcccgaacca
      121 cgtggccgcc gcgacgcccg cgcgggcgag ggccgcctcg gcgtcggcga cgatggcctc
      181 ggggctcgtg tagaccagcg ccttgtcgtc gcggaggcgc gcgtagatgt ccgagacctc
      241 cgcgagtccg aagagcgggc cggcgatcgc gcggtactcg tcctcgagtt ccgcgacctt
      301 gtcgagcccg atctggtgaa cctcttgggc cgtgcggtcg gtcagcaggt tggcccacat
      361 cgcgtcgcga tagacctcga ggccgccggg gagattgcac agccctggct tctcgtcggg
      421 gcgggcgtct ctggccaggt cgcggagcgc ggcgccgtag gccaccaggc cctctcggac
      481 cgtcgtcgcg atgatcgcgt cgcgggcctc gcgccacgcg cccgcctcgt ccgcgccgag
      541 cttggtgggt gcctcctggc aacacaggcg ctcgcccggg ccgggagggc tcgcgaggta
      601 cgcgtcgatc gagtccgcgg tcgcgcccag ctgccgggcc gtcgcgacgc gcccctcggc
      661 cgcggcggca cgcgcggcgt cggcgaggac gcgcagcgcg cccggcatgc ggcggagttt
      721 ctcgaggtac tgctcgccgt gctccgcggt tttcaggggg taggtcccga tgaacgacag
      781 gcacatctcg aacaggccca tcttcgggga gacctggagg agttccgcct cccaggtgag
      841 cctgatcgcg gcggaccggg cgcccgtcga gatggagtcg cggagcgcga tgtcgcgggg
      901 gtcgtcgccg atctggagcg catccgccgc ctccgcgaag gcgaggtacc gcgccttgcg
      961 gcgcccgacg gccgcgggcg agaagtcgtc ccactccgcg aggtggtcga gctttccgtt
     1021 ccacatcaat ctcagggggc tgtcgctcgt cgcgtaggcg tagtagtcgt ccgccagctt
     1081 cgtcaatgcg tccatgagcc gactctagcc aggcgcccga tacactgtct tcaccagcgt
     1141 cgacccggtt atcgccggcg agcttccgga agaacggccc cccgccgaag ccaggcgcgg
     1201 agccagtaga accggacggg cagcccgtca cagcgtctcg agtggccgag cccttcgcgg
     1261 ggcgaggcaa gcggggtggt accgcgtcgc tcgccctccc gggcgtcggc gtccccgtac
     1321 aggcagggat cgagacgcac gaggacgccc cacatgaccg aagcatcggc agacaagtac
     1381 ccgctccacc gcagtcacct cgacggcgcg gacggcgagg tcgcggcctc gccgcgcttc
     1441 cccgcgatcg agcgggacgt cctcgcgtac tgggagagcg acggcacctt ccaggcttcg
     1501 atcgacaacc gcgccggcac ccccgaacac cccgccgagg agttcgtctt ctacgacggc
     1561 ccgcccttcg ccaacggcct cccgcactac gggcacctgc tgaccggtta cgccaaggac
     1621 gtggtgcccc gcttcgagac gatgcgcggc aagttggtgg agcggcgctt cggctgggac
     1681 acgcacgggc tccccgccga gctcgaggcc gagcggatcc tcggcatcac ggacaaggcg
     1741 cagatcgagg agatgggcat cggggcgttc aacgacgcgt gccgcgagtc cgtgctgaag
     1801 tacaccaagg agtgggagga atacgt
//
LOCUS       DASVKP010000227         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_150192, whole genome shotgun sequence.
ACCESSION   DASVKP010000227 DASVKP010000000
VERSION     DASVKP010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150192"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VF362_04515"
     CDS             <1..596
                     /locus_tag="VF362_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEX7590224.1"
                     /translation="GMALAAVGCVVVAVAPAALGPALTRVASTLPSVGGEQGPKLGAI
                     LRLPGAAGSVSPAILAAAFAVAISLVLLLARWGVGARPAAVVRPLWAGGGPALTPRMQ
                     YTATSFAEPLQRVFDDVLRPDTDVAVTHVAESRYLIERITFHTSQADAVEEHLYWPVV
                     RGVAAAARGVRRAHTGSIHLYLLFGAVGLLVVIVVGR"
     gene            593..1555
                     /locus_tag="VF362_04520"
     CDS             593..1555
                     /locus_tag="VF362_04520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX7590225.1"
                     /translation="MMALSIALAALQVLMVLGGAPLVIGAMRQVRARLEGRRGGGLGQ
                     PWRDLRKLFRKQQITPEGTTVVFRAAPFILLGSTLLIAAIIPVVSLGSPLDSSGDFVA
                     VVGLLFLGTVALALAALDTGTAFGGMGSSRQMTVVALVEPTILLVLFTLSLRVHSANL
                     AEIASGTLANPAQAFSLGRILAFASLVIVIIAETGRLPVDNPATHLELTMIHEAMTLE
                     YAGPRLGLVEWASGMRLTVLLVLLANLFFPWGVAPGGPGLVQVLIAVAAIAAKVAILA
                     MALSVGETFLAKLRLFRVPELLAGSFLLGILAVTASSFLPAAGR"
     gene            1569..>2062
                     /locus_tag="VF362_04525"
     CDS             1569..>2062
                     /locus_tag="VF362_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590226.1"
                     /translation="MITATYTGILTLAAGSFLLAAVLIVWRRGLRATISLLAWQGVAL
                     ATIPIANGVHQRAWMPIAVGIVVLALRGVLLPLLLARAVRGEDQGAREPTPLVNTTAA
                     LLVTAALTMIAYAATRPLIQHSPTPGTRAAPAAFAALLIAVFVMVSRRRAMSQAIGFL
                     MLDNG"
BASE COUNT          223 a          801 c          682 g          356 t
ORIGIN      
        1 tcggaatggc gctcgcggcg gtcggctgcg tcgtcgtcgc ggtggccccg gccgcgctcg
       61 gccccgccct cacgcgcgtc gcctccaccc tgccgagcgt cggcggagag cagggcccca
      121 agctcggcgc catccttcgg cttcccggcg ccgccggatc cgtctcgccc gcgatcctcg
      181 ccgccgcgtt cgccgtcgcg atctcgctcg tcctgctcct cgcccggtgg ggcgtcggcg
      241 cccggcccgc cgccgtcgtg cgaccgctgt gggcgggggg tggccccgcg ctcacgccgc
      301 gcatgcaata caccgcgacc tccttcgccg agccgctcca gcgggtgttt gacgacgtcc
      361 ttcgcccgga caccgacgtc gcggtgacgc atgtggccga atcgcggtac ctgatcgaga
      421 ggatcacctt ccacacatcg caggcggatg ccgtcgagga gcacctgtac tggcccgtgg
      481 tgcgcggagt cgccgccgcc gcgcggggcg tccggagggc ccatacgggg agcatccacc
      541 tctacctcct cttcggtgcg gtcggtctgc tcgtcgtcat cgtcgtgggg cgatgatggc
      601 gctctcgatc gcgctcgcgg cgcttcaggt cctcatggtc ctcggcggcg cgcccctggt
      661 gatcggggcg atgcgccagg tccgcgcccg gctcgagggg cgacggggcg ggggcctcgg
      721 ccagccctgg cgcgacctgc gcaagttgtt ccgcaagcag cagatcacgc ccgaggggac
      781 caccgtggtc ttccgcgcgg caccgttcat cctgctgggc tcgacgctgc tcatcgcggc
      841 gatcatcccc gtggtgagcc tcgggtcgcc gctcgattct tcgggcgact tcgtcgcggt
      901 cgtggggctg ctcttcctgg gaacggtcgc gctcgccctc gccgccctcg ataccggcac
      961 cgcgttcggc ggcatgggat cgagccgaca gatgaccgtg gtcgcgctcg tcgagccgac
     1021 catcctcctc gtgctcttca ccctctcgct tcgcgtccac tcggcgaacc tcgccgagat
     1081 cgcgagcgga accctggcga accccgcgca ggccttttcg ctcgggcgca tcctggcgtt
     1141 cgcgtcgctc gtgatcgtca tcatcgcgga gaccggccgc ctccccgtcg acaaccccgc
     1201 cacccacctc gaactcacga tgattcacga ggcgatgacg ctcgagtacg cgggtccccg
     1261 cctcgggctc gtggaatggg cgagcgggat gaggctgacg gtgctcctcg tgctgctcgc
     1321 caacctcttc ttcccgtggg gtgtcgcgcc gggcgggccc ggccttgtcc aggtgctcat
     1381 cgccgtcgcc gcgatcgccg cgaaggttgc catcctcgcg atggcactct cggtgggcga
     1441 gaccttcctc gccaagctac ggctcttccg cgttcccgag cttctcgccg ggtccttcct
     1501 cctcggaata ctcgcggtga ccgcgagctc cttcctcccg gcggcgggtc gctgatgggg
     1561 gcgctcgcat gatcaccgcg acctacaccg ggatcctcac gctcgcggcc ggctccttcc
     1621 tccttgccgc cgtcctcatc gtctggcggc gcgggctccg cgccacgatc agcctcctcg
     1681 cgtggcaggg cgtcgcgctg gcgaccattc ccatcgcgaa cggggtgcat cagcgcgcct
     1741 ggatgccaat cgcggtcggc atcgtcgtgc tggcgctccg aggcgtcctc ctgccgctcc
     1801 tgctcgcccg cgccgtccgc ggcgaggacc agggcgcccg tgagccgacg cccctggtca
     1861 acacgaccgc ggcgctcctc gtgacggcgg cgctcacgat gatcgcgtac gccgccacgc
     1921 ggcccctgat ccagcactcg cccacccccg gcacgagggc ggcgccggcg gctttcgcgg
     1981 cgctcctcat cgccgtcttc gtcatggtga gccgcaggcg cgcgatgtcc caggccatcg
     2041 ggttcctcat gctcgacaac gg
//
LOCUS       DASVKP010000228         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1061177, whole genome shotgun sequence.
ACCESSION   DASVKP010000228 DASVKP010000000
VERSION     DASVKP010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061177"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VF362_04530"
     CDS             complement(<1..280)
                     /locus_tag="VF362_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009055773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX7590227.1"
                     /translation="MGTTLAEKLWQSHLVREGEGGAPDLLYIDLHMVHEVTSPQAFDG
                     LRLSGRKVRRPDLTIATEDHNTPTLDIDKPIADLTSRTQIDTLRANAKE"
     gene            404..1123
                     /locus_tag="VF362_04535"
     CDS             404..1123
                     /locus_tag="VF362_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7590228.1"
                     /translation="MDNHSGVGVIDKAAAILAELEKGPATLADLVARTHLARPTVHRL
                     ALALEHHHLVGRDARGAFILGRRFLQLAATVGEDRLVSAAQPILEALRDRTEESAQLF
                     RPHAEHRICIAAADRPFGLRDSIPVGTTMTMHAGSAAQVLLAWEEPERMHALLEGARF
                     TASDLARVREQGYAESAGEREAGVAGVSAPVWGASGRVVAAVSISGPIARLTGHPAEK
                     HARDVTDAARRLSDVLRKSDS"
     gene            complement(1120..>1651)
                     /locus_tag="VF362_04540"
     CDS             complement(1120..>1651)
                     /locus_tag="VF362_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590229.1"
                     /translation="MKAVVVYQSLWGSTAAIARAIAEGLGTGAVALTTNEATPEAIAG
                     ANLIVAGCPVHAFNLPSVATVEQAVAKPVGEKAVVADSSHRLMRDWLEELHKGSGRSA
                     AFDTRVRGPLGKGGASKIAARLEGAGYSSLVPREGFFVAMKGAEVTKDGMLLPGEVDR
                     AREWGRAMAAAMAEAR"
BASE COUNT          230 a          599 c          569 g          253 t
ORIGIN      
        1 actccttggc gttggcgcgg agcgtgtcga tctgcgtccg gctcgtgagg tcggcgatgg
       61 gcttgtcgat gtcgagcgtc ggggtgttgt ggtcctccgt cgcgatcgtg aggtcggggc
      121 ggcgcacctt ccggcccgag agccgcaacc cgtcgaacgc ctgggggctc gtgacctcgt
      181 gcaccatgtg gaggtcgata tacagcaggt cgggggcgcc gccttctccc tcgcggacga
      241 ggtggctctg ccacagcttt tcggcaagcg tcgtgcccat gtgcgttcct cccggctcaa
      301 gggcgcccga gtcgcggcgg cctcccgatc gatcatgctg gcgtcaaaca tactggcttc
      361 aaatgtgctt gtgtctcact atgtaggacg ctagtatcgt cctgtggaca atcatagcgg
      421 agtcggcgtt attgacaagg ccgcggcgat ccttgcggag ctcgagaagg gaccggcgac
      481 gctcgccgac ctggtggcgc gcacgcacct cgcccgcccc acggtccacc gcctcgcgct
      541 cgcgctcgaa caccaccacc tcgtcggccg cgacgcccgc ggggcgttca tcctgggccg
      601 ccgcttcctc cagctcgccg ccacggtggg cgaggaccgc ctcgtgagcg ccgcgcagcc
      661 catcctcgag gccctgcggg accgaacgga ggagtcggcg caactcttcc gcccccacgc
      721 ggagcaccgc atctgcatcg ccgccgcgga tcggcccttt gggttgcgcg actccatccc
      781 cgtcggcacc acgatgacca tgcacgcggg gtccgcggcg caggtgctac tcgcgtggga
      841 ggagccggag cgcatgcacg cgctactcga gggtgcgagg ttcaccgcca gcgacctggc
      901 gcgggtgcgc gaacagggct acgccgagag cgcgggggaa cgcgaggcag gcgtggccgg
      961 agtctccgcg cccgtgtggg gggcgtcggg gcgcgtcgtc gcggccgtct cgatctccgg
     1021 tccgatcgcc aggctcacgg gccacccggc cgagaagcac gcgagggacg tcacggacgc
     1081 ggcacgacgc ctcagcgacg tcctccgcaa gagcgactcc tagcgcgcct cggccatggc
     1141 cgcggccatc gcgcggcccc actcgcgggc gcggtcgacc tcccccggca ggagcatccc
     1201 gtccttggtg acctccgcgc ccttcatcgc cacgaagaag ccctcgcgcg gcaccaggct
     1261 cgaatagccg gcgccctcga gccgcgccgc gatcttcgac gcccctccct tgccaagggg
     1321 gccgcgcacc ctggtatcga acgcggcgga acgaccggac cccttgtgga gttcctcgag
     1381 ccaatcacgc atcaggcggt gcgaactgtc ggcaacgacc gccttctctc ctacgggctt
     1441 cgcgacggcc tgctcgaccg tcgcgacgga cgggaggttg aaggcgtgaa ccgggcagcc
     1501 cgccacgatg agattcgccc cggcgatggc ctctggggtc gcctcgttgg tggtgagcgc
     1561 gaccgcaccg gtcccgaggc cctccgcgat ggcccgcgcg atcgcggcgg tgctgcccca
     1621 cagcgactgg tagacgacga cggccttcat g
//
LOCUS       DASVKP010000229         7255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_981838, whole genome shotgun sequence.
ACCESSION   DASVKP010000229 DASVKP010000000
VERSION     DASVKP010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981838"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VF362_04545"
     CDS             <1..719
                     /locus_tag="VF362_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7590230.1"
                     /translation="SGDVVLGVARHEIAVLSDTPQFDKWLTGREVVALSLELASPGAD
                     AARVDHVLAETGLTEAAGRRVGGYSRGMLQRLGIAATLVSRPRLLLLDEPASALDPQG
                     RREVLDLITRLRGTATVLFSSHILADVQEVCDTVGILRGGRLLFQGPLHQLLVGSAVP
                     RYEIRLRGDTAPAIAALRRAPWVTQVREFDAGEIVVEVRSLQDAEVGLPRALAEAGAS
                     VVSLRPEEVSLERAFLELTQ"
     gene            734..1489
                     /locus_tag="VF362_04550"
     CDS             734..1489
                     /locus_tag="VF362_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590231.1"
                     /translation="MTLWRLEILRVLRTRRWIALGAVYVGFGLLGPLSAKYLAEILKL
                     SGTGADGVTITFPPPVPADGMASYVGNALQVGTIVAVVVVAGALAFDAIPEMGVFLRT
                     RVTSVWGILIPRLVVSFALCSAAFVLGTATAWYETWALLGPLSVSAVAWGTLLGILFT
                     AFLVALVAAIAQWAKGVLGTVLVSLVVLIALPILGVVHAIQVWLPTRLGGALTELTSG
                     TLASDFWRSSLVAVLAIAGLLALAAWGARRREI"
     gene            1679..2428
                     /locus_tag="VF362_04555"
     CDS             1679..2428
                     /locus_tag="VF362_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7590232.1"
                     /translation="MTKTTTPVWTEQASDADLIRGVREGDRDAYRVLFERHASAARRV
                     AFAYTSVSADVDDIVSDAFEAVLRALQNGNGPTESFRAYLFTVIRRAARDVKRHAERT
                     RPCEDMAVHEHALGRLAPSSDPTIAKFEHATVAEAFASLPERWQIVLWNTEIEKKTPA
                     EIAPSLGLTPNGVAALAYRAREALRQAYLQEHLAAVEDTACLSVVQDLAGFVRGSLTR
                     RDLEYVTAHLRVCQNCRELVAELEDVNAMLR"
     gene            complement(2425..3513)
                     /gene="mnmA"
                     /locus_tag="VF362_04560"
     CDS             complement(2425..3513)
                     /gene="mnmA"
                     /locus_tag="VF362_04560"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX7590233.1"
                     /translation="MKVLAALSGGVDSAVAAARAVDAGHDVVGIHMALSRNRDEFRTG
                     SRGCCSLEDSDDARRAADRLGIPYYVWDLSDEFEDRVVADFLSEYASGRTPNPCVRCN
                     QHIKFEALLGRASALGFEAVCTGHYARVVLRDDGTRELHRSIDAAKDQSYVLAVIGAE
                     RLSLAMFPLGEAASKAEVRAEAQARGLGVSHKPDSYDICFIADGDTQGFLQRRLGGRP
                     GDIVDQAGAVVGSHGGAYAFTVGQRKGLRLGRPAADGKPRYVTEVRVDANVVVVGSET
                     LLSVTRLEGDEVVWLAADEPAEVPTRCVVQVRAHGEPAPGRVTVAGGLMLVELDAPMR
                     GVAAGQTMVAYAGTRVLGQARVGARSAP"
     gene            complement(3510..4724)
                     /locus_tag="VF362_04565"
     CDS             complement(3510..4724)
                     /locus_tag="VF362_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005270317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX7590234.1"
                     /translation="MASHYLDHAATTPMFAAAIEAYVDEVGHPGNPSALHRSGRLARA
                     VVEDGREAIARALGAAPAEVIFTSGGTEADNLAIKGLWWARTAEDPRRTRVVTTAVEH
                     HAGLNSVRWIAAERGATAIVLPVDRDGIVDMDALRDEVAAHGDEIALISVMWANNEVG
                     AVQPVDEVVAVASARGIPVHSDAVQAIGHVPFDFGRSGLATAASSAHKFGGPVGVGAL
                     LAGKSIALTPLAHGGGQERKVRSGTLNAAGVRAMSVAAALAVGSVSSESSRLADLQRD
                     LIAGVRALVPDAVLSGPEPGRGRLPGIAHFVFPGCAAEAMMYVLDSRGVEASNGSACT
                     AGVAQASHVLLAMGRTPSEAASTMRFSLGHTSTREDVAALLEHLPEAIAKARRIGGGL
                     RAVAPAGGGSGR"
     gene            4822..5274
                     /locus_tag="VF362_04570"
     CDS             4822..5274
                     /locus_tag="VF362_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590235.1"
                     /translation="MSAHTRIRRPDPRTLGWSKGAGFADATSAVAQGGEGGRVTREEP
                     RRWDWWLAGCGALAAILVVIDVAAVVSDDVAAGAVSAPRTSATAASSSGEAAVDIPSS
                     RVPVPHAERAFTGECEGFLARGLPSGSHAPAAASATRVWNSAIRSGLC"
     gene            5558..6202
                     /locus_tag="VF362_04575"
     CDS             5558..6202
                     /locus_tag="VF362_04575"
                     /inference="COORDINATES: protein motif:HMM:NF039508.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5701 family protein"
                     /protein_id="HEX7590236.1"
                     /translation="MSHSQALFTQQLDAYVAAGYDDLVDLTESEFRARLEPLRTVLDR
                     AVADGLALDSSADHVPFVVVVTSRVVPPEARVSRLRVPGSDREGAIDPGHRDGLAAYR
                     PIPGLPLPAAEAYLLVDVDRGDTLREIAADDALRAIAATNRSPLTLDEGLSLATVHPL
                     ALEADAGFLLAGSRHRRRVPALWVTAGGAMLGWGGGATPEAWLGVASAAARVAP"
     gene            6238..>7255
                     /locus_tag="VF362_04580"
     CDS             6238..>7255
                     /locus_tag="VF362_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7590237.1"
                     /translation="MSAIPSPTPHRFGVHLGSAGASVSVWAPHATGVDLCLFDARGAE
                     SRIPLVGPEAGAWHAWVPEIRAGQRYGFRARGPWDPSQGHVYNQARLLLDPYARGIEG
                     AIAHESTDGSAAFLSRQDDLDSAPFVPRGVVLAPQRTPAEPGPRVPWADTIVYEAHVK
                     GLTHAMPGIPAALRGTYAALGHDAAVKYLQGLGVTTLELLPIHAFASEMHLERLRLNN
                     YWGYNTLGFFAPHSSYATDAARGKGPQGVADELRAAIAALHRGGIEVILDVVYNHTCE
                     GGAGGRQVSWRGLDNRAYYRHLDGQPGALDDTTGTGNTLDFGEPRVVQMALDSLRYWI
                     TDYGV"
BASE COUNT         1039 a         2685 c         2459 g         1072 t
ORIGIN      
        1 cctcgggaga cgtcgtcctc ggagtcgcgc ggcacgagat cgcggtccta tccgataccc
       61 cgcagttcga caagtggctg acgggacgcg aggtcgtcgc gctctcgctc gaactcgcct
      121 cgccgggcgc ggatgcggcc cgcgtcgacc atgtgctcgc cgagacgggc ctgaccgagg
      181 ccgccgggcg tcgcgtcggc gggtactctc gcggcatgct ccagcgcctt ggcatcgccg
      241 cgaccctcgt ctctcggccg cgcctgctcc tcctcgatga gccagcgtcg gccctcgacc
      301 cgcaggggcg gcgagaggtt ctcgacctga tcacgcgcct gaggggcacc gcgaccgtcc
      361 tgttctccag ccacatcctg gccgacgtgc aggaggtgtg cgacaccgtc ggaatcctcc
      421 ggggagggcg cctcctgttc cagggtcccc tccaccaact cctcgtcggc tcggcggtgc
      481 cgcgctacga gattcggctc cgcggcgaca cggcgcccgc catcgcggcg ctccgcagag
      541 cgccgtgggt cacgcaggtc cgcgagttcg acgccggaga gatcgtggtc gaggtgcgtt
      601 cgctgcagga cgccgaggtg ggcctccctc gtgcgctcgc cgaggcgggc gcctcggtcg
      661 tctccctgcg gccggaagag gtctcgctcg aacgtgcctt cctggagttg acccaatgac
      721 cggagttggc ccgatgacgc tgtggcgcct ggagatcctc cgcgtgctgc ggacgcggag
      781 atggatcgcg ctcggggccg tgtacgtagg attcgggctc ctaggaccgc tgagcgccaa
      841 gtacctcgcg gagatcctca aactctcggg aacgggcgcg gacggcgtca cgatcacctt
      901 ccctcccccg gtcccggccg acggcatggc aagttacgtg ggcaacgcgc tgcaggtggg
      961 cacgatcgtc gcggtcgtgg tggtcgcggg cgcgctcgcc ttcgacgcga tccccgagat
     1021 gggcgtgttc ctcaggaccc gcgtcacgag cgtatggggc atcctcatcc cccgcctcgt
     1081 cgtgtcgttc gcgctgtgct cggcggcctt cgtcctgggc acggcgacgg cctggtacga
     1141 gacgtgggcg ctcctggggc ccctcagcgt ttccgcggtc gcatggggaa ccctcctcgg
     1201 catcctgttc accgcgtttc tcgtcgccct ggtcgccgcg atcgcgcagt gggccaaggg
     1261 cgtgctggga acggtactgg tttcgctcgt cgtcctgatc gcccttccca tcctcggcgt
     1321 cgtccacgcg atccaggtgt ggctccctac gcgcctcggc ggcgcgctca cggaactcac
     1381 gtcgggaacg ctggcgagcg acttctggag gtcgtcactc gttgccgttc tcgccatcgc
     1441 cggactcctc gcgctcgccg cgtggggcgc gcgtcgacgc gaaatctagg ttcgtgcagc
     1501 cccctttccg gctcttcccg cccccaaggc ctccaggacg tggcccccgt cacgtcctga
     1561 ttgtggccct catctcttcc cgccttggcc tgtgacgcac ccaccacccc aatggttgaa
     1621 agcgctttcc cgcgggttga ggatgagagt gagagcaaga aagagggcgg tgagaacaat
     1681 gactaagaca actaccccgg tctggaccga acaagcgagc gacgccgatt tgattcgcgg
     1741 cgtccgcgag ggagaccgcg acgcgtaccg cgtcctgttc gaacgccacg ccagtgccgc
     1801 gcgccgagtc gccttcgcct atacctcggt atcggcggac gtcgatgaca tcgtctccga
     1861 tgccttcgag gcggtcctcc gcgccctcca gaacggcaac ggccccacgg agtccttccg
     1921 cgcctacctc ttcaccgtca tccgccgcgc cgcgcgcgac gtcaagcgcc acgccgagcg
     1981 gacgagaccg tgcgaagaca tggccgtcca cgaacacgcc cttgggcgcc tcgcgcccag
     2041 cagcgacccg accatcgcca agttcgaaca cgccaccgtc gccgaggcct tcgcctcgct
     2101 cccggagcgc tggcagatcg tcctgtggaa cacggagatc gagaagaaga cgcccgccga
     2161 gatcgcgccg agcctggggc tcacccccaa cggcgtcgcg gcgctcgcct accgcgcccg
     2221 cgaagccctg cgccaggcct acctgcagga acacctggcc gcggttgagg acacggcgtg
     2281 cctctcggtc gtccaagacc tcgccgggtt cgtgcgcggc tcgctcacga ggcgcgacct
     2341 cgagtacgtc accgcacacc tgagggtgtg tcagaactgc cgcgaactgg tggccgaact
     2401 ggaggacgtc aacgcgatgc tccgctaggg agcggatctc gcgccaaccc tcgcctgtcc
     2461 caacaccctc gtccccgcat acgcgaccat cgtctggccc gcggcgacgc cccgcatggg
     2521 cgcgtcgagt tccacgagca tgaggccgcc cgcgacggtg acgcggccgg gcgcgggctc
     2581 gccgtgcgcg cgcacctgga ccacgcaccg cgtcggaact tcggcgggct cgtcggcggc
     2641 gagccacacg acctcgtcac cctcgagccg cgtcaccgag agcagcgtct cggacccgac
     2701 gaccacgacg ttcgcgtcca cgcgcacctc cgtgacgtag cgaggcttgc catcggcggc
     2761 ggggcgcccg aggcgcaggc ccttgcgctg gccgacggtg aacgcgtagg cgccgccgtg
     2821 ggatccgacc acggccccgg cctggtcgac gatgtcgccc ggccgcccgc cgagccgacg
     2881 ctgcagaaag ccctgggtgt cgccgtcggc gatgaagcag atgtcgtagg aatcgggctt
     2941 gtgcgacacc ccgaggccac gcgcctgggc ctccgcgcgc acctccgcct tggacgccgc
     3001 ctccccgagg gggaacatcg cgagggagag gcgctccgcc ccgatgaccg ccaggacgta
     3061 ggactggtcc ttcgcggcgt cgatcgagcg atgaagctcc ctcgtgccgt cgtcgcgcag
     3121 caccacgcgc gcgtagtgcc cggtgcacac ggcctcgaaa cccagggccg aggcgcgccc
     3181 gagcaatgcc tcaaacttga tgtgctggtt gcagcgcacg cagggattcg gcgtccggcc
     3241 cgacgcatac tcggacagga agtcggccac gacccggtcc tcgaactcgt cgctgaggtc
     3301 ccacacgtag taggggattc cgaggcgatc ggccgcccga cgcgcgtcgt cggagtcctc
     3361 gagcgagcaa cagccccgcg atccggttcg gaactcgtcg cggttccgac tgagggccat
     3421 gtggatcccg acaacgtcgt gaccggcgtc cacggcgcgg gccgcggcca cggcggaatc
     3481 gaccccgccg gagagcgcgg cgagcacctt catcggcccg acccaccgcc cgccggggcg
     3541 acggcgcgca agccaccgcc gatgcgcctc gccttcgcta tcgcctcggg aaggtgctca
     3601 aggagcgccg cgacatcctc gcgggttgat gtgtgcccaa gcgagaagcg catcgtcgag
     3661 gccgcctcgc tcggcgttct gcccatggcc agaaggacgt gactcgcctg cgcgactccg
     3721 gcggtgcagg ccgagccgtt ggacgcctcg accccgcgcg aatcgagcac gtacatcatg
     3781 gcctcggcgg cgcagcccgg gaagacgaag tgggcgatgc cggggagccg accgcggccg
     3841 ggctccggtc ccgagagcac cgcgtccggc acgagcgcgc gcacccccgc aatgaggtcg
     3901 cgctggaggt cggcgaggcg ggaagactcc gacgacacgg accctacggc cagggccgcc
     3961 gcgaccgaca tcgcgcgaac gccagcggcg ttgagggtcc ctgaccgcac cttgcgctcc
     4021 tggcccccgc cgtgggccaa gggagtgagg gcgatactct tgcccgcgag tagcgcgccg
     4081 accccgacgg gaccgccgaa cttgtgtgcg cttgacgcgg cggtcgcgag ccccgaacgc
     4141 ccgaaatcga acgggacgtg cccgatcgcc tgcaccgcgt cggagtggac cggaatgccg
     4201 cgggccgagg cgacggcgac aacctcgtcg acgggctgga cggccccaac ctcgttgttc
     4261 gcccacatca ccgagatgag cgcaatctcg tcgccgtggg ccgcgacctc gtcgcggagc
     4321 gcgtccatgt cgacgatgcc gtcccgatcg acgggaagga cgatcgcggt cgcgccgcgc
     4381 tccgccgcga tccagcgcac cgagttgaga cccgcgtggt gttcgacggc cgtcgtcacc
     4441 acccgggtgc gacgggggtc ctcggccgtg cgcgcccacc acagcccctt gatcgcgagg
     4501 ttgtccgcct cggtcccccc ggaggtgaag ataacctcgg cgggcgccgc gcctagggcc
     4561 cttgcgatcg cctccctccc atcctcgacg accgccctcg cgagcctgcc cgagcggtgg
     4621 agggcgctcg gattccccgg gtgccccacc tcgtccacgt acgcctcgat cgccgccgcg
     4681 aacatcggag tcgtggcggc gtggtcgagg tagtgcgagg ccatccggcg attctacggg
     4741 ccgtcgcgcg ggcggctccg tagaggagtc aacgagcgtg ttggcgttcc tcaaccgacg
     4801 cgctgtgaca tcggtcacag tgtgagcgcg cacacgagaa ttcgccgccc cgatccccgt
     4861 accctcgggt ggtcgaaggg ggcgggcttc gcggacgcga ccagcgccgt cgcccagggc
     4921 ggggaaggtg gacgcgtgac gcgcgaggag ccgcggcggt gggactggtg gctagccggt
     4981 tgcggcgcgc ttgccgcaat cctcgtcgtc atcgacgtcg ccgccgtcgt ctcggacgat
     5041 gtcgcggcgg gagccgtttc cgcacctcga accagcgcca cggccgcttc ctcgtcgggc
     5101 gaggcggccg tcgacatccc ttcttcccgg gtgccggtgc cccacgcgga gcgcgccttc
     5161 acaggcgagt gcgagggctt ccttgcgcga gggctcccct cgggatccca cgcccccgcc
     5221 gccgcttcgg ccacgcgtgt ctggaactcg gcgattcgct cgggactctg ctgactcacc
     5281 cgggactctc gcctagcgcc gtgaggccat gttgcggcga gcgcgccgcc gaccgcatgc
     5341 gcagtttcat gggaagtcgg aacttcgcgg gggctcctcc tcgccgccac ggaatccggc
     5401 catccgtgaa cgagcgctcc aacggacgat cgggcacggg cttcacgagc gcgaggaccc
     5461 gcgcgcggca accctaccca ggcaagggaa cggccgggcc gccccggccg ttcacgggac
     5521 gcccaccaaa ccgaaccccg ggaggacaat ggacaccgtg agtcactcac aggccctgtt
     5581 cacccaacag ctcgacgcct acgtcgctgc gggctacgac gaccttgttg acctcaccga
     5641 atcggagttt cgggcgcgcc tcgagccgct ccggacggtc ctcgaccggg cggttgccga
     5701 cggtctcgcc ctggattcga gcgccgacca cgtccccttc gtcgtagtcg tcacctctcg
     5761 cgtcgtccca cccgaagccc gcgtttccag gctccgagtc ccgggcagcg acagggaggg
     5821 cgccatcgac cccgggcata gggacggcct tgcggcctac cgcccgatcc ctggactccc
     5881 cctcccggcg gccgaggcgt acctcctcgt ggatgtcgat cgcggcgata ccttgaggga
     5941 gattgcggcg gatgacgcgc tcagggcgat cgcggcgacc aacaggagcc cgctcacgct
     6001 cgacgagggc ctctctctcg ccaccgtgca tcccctcgct ttggaggccg acgccggctt
     6061 cctcctcgcc ggctcgcggc atcggcgtcg cgttcctgcg ctgtgggtca cggcgggcgg
     6121 tgcgatgctc ggctggggcg gtggcgcgac gcccgaggcg tggctcggcg ttgcgtcggc
     6181 ggcggcgcgc gtagcgccgt agcttccctg ccacgcgacc gggggagaat ggtggccatg
     6241 tccgccatcc ccagcccaac cccccatcgg ttcggcgtcc acctcggctc ggccggggcc
     6301 tccgtctcgg tatgggcgcc ccatgccacg ggcgtcgatc tctgcctgtt cgacgcgagg
     6361 ggcgcggaga gccggattcc gctcgtggga cccgaggccg gggcgtggca cgcctgggtt
     6421 ccagagatca gggcggggca gcgctacggc ttccgcgcca ggggtccgtg ggatccctcc
     6481 cagggccacg tatacaacca ggctcgcctc ctcctcgacc cctatgcccg cggcatcgag
     6541 ggcgccatcg cgcacgagtc gacggacggc tcggcggcct tcctgagccg ccaagacgac
     6601 cttgactcgg caccgttcgt cccgcggggc gtggtgctcg cgccccagag gactccggcc
     6661 gagcccgggc cacgggtccc gtgggcggac acgatcgtct acgaggccca cgtcaagggt
     6721 ctgacccacg ccatgcccgg tattcccgcg gctttgcggg gcacctacgc cgcgctgggc
     6781 cacgacgccg ccgtgaagta cctgcagggc ctgggcgtga cgacgctcga gctcctccca
     6841 atccacgcgt tcgcgagcga gatgcacctc gagaggcttc gcctcaacaa ctactggggc
     6901 tacaacacgc tcggcttctt cgcgccgcac tcgtcctacg cgaccgacgc cgcccgaggc
     6961 aaggggcccc aaggggtcgc ggacgaactc cgcgcggcga tcgccgcgct ccaccggggc
     7021 gggatcgagg tcatcctcga cgtcgtgtac aaccacacgt gcgaaggggg cgcgggcggc
     7081 cgccaagtgt cgtggagggg cctcgacaat cgcgcctact atcggcacct cgatgggcag
     7141 cccggcgcac tggacgacac gaccggcacg ggcaacacgc tcgacttcgg cgagccgcgc
     7201 gtcgtccaga tggctctcga ctccctgcgg tactggatca ccgactacgg agtgg
//
LOCUS       DASVKP010000230         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_489214, whole genome shotgun sequence.
ACCESSION   DASVKP010000230 DASVKP010000000
VERSION     DASVKP010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489214"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..507)
                     /locus_tag="VF362_04585"
     CDS             complement(55..507)
                     /locus_tag="VF362_04585"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7590238.1"
                     /translation="MATAAVFNREIIAEPETVWRTVTDIDRWPRILSSLASLDRLEGT
                     GLAPGVRWRETRLILGVTAVVEMTVTDVQEGKSFTLAASADDATIGLSYRFKPSSLGT
                     RLEATVNADGSGAGLGKRLRGVIAGGGAKLAREMIERDLADFQAALRT"
     gene            973..1908
                     /gene="cysK"
                     /locus_tag="VF362_04590"
     CDS             973..1908
                     /gene="cysK"
                     /locus_tag="VF362_04590"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX7590239.1"
                     /translation="MAKIYDDATQLIGHTPLVRINKLTKGSGAIVLAKLEFYNPASSV
                     KDRIGVAIIDAAEKDGSLKPGGTIVEGTSGNTGIALAFVGAVRGYKVVLTMPETMSKE
                     RRALLRAFGAELILTPGPAGMKGAVEEAERIAKDRPGAVLARQFANLANPAIHRTTTA
                     VEIWDDTDGKVDILIAGVGTGGTITGVGEVLKARKPSVQVIAVEPAESPLLNGGEPGP
                     HKIQGLGANFIPEILNTKIYDEVIDVDSETAIATARAAAREEGLLVGISSGAALHAAS
                     IVAKRPENKGKTIVVIIPSFGERYLSTVLYADLME"
     gene            2007..>2217
                     /locus_tag="VF362_04595"
     CDS             2007..>2217
                     /locus_tag="VF362_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX7590240.1"
                     /translation="MTQHQSQRHIGPLARLVEDVRAAHDRDPAARSTLVIVASYQGVH
                     ALWYHRAAHWMWTHGMRIPGRLLSQH"
BASE COUNT          360 a          801 c          728 g          328 t
ORIGIN      
        1 tgcccatcaa ggtgagcgag tagccagccc ggctgactcg cgaactgcgg gccatcacgt
       61 gcgcagggcg gcctggaagt cggcgaggtc gcgctcgatc atctcgcgcg cgagttttgc
      121 gcctccaccg gcgatcacgc ccctgaggcg cttccccagc ccggcgcccg acccgtccgc
      181 gttgaccgtg gcctcgagcc gcgttccgag cgacgacggc ttgaatcgat aggacaggcc
      241 gatcgtcgcg tcgtccgcgc tcgccgcaag cgtgaagctc ttgccctcct gcacgtcggt
      301 caccgtcatc tcgacgacgg ccgtgacccc caggatcagt cgcgtttccc tccaccgaac
      361 cccgggcgcg aggcccgttc cctctagtcg gtcgagcgac gcgaggctcg agaggatgcg
      421 cggccatcgg tcgatgtccg tgaccgtcct ccagacggtc tctggctcgg cgatgatctc
      481 acggttgaat acggcggcgg tggccacgat gttctccttt ccgcaccatt gcggatatcc
      541 ccgagggtac tcgcgacgaa ggggcgccgc gcgccgtctg agggcgccgg ggtgagtgcg
      601 taccgcgcag gcggttgcac cgcgccccgg agcggcgtta ccctggcacc acccacgatg
      661 ggagggaacg gcatgagcga caccgcgctc cgcgtcacgt gcgcgacttc gtcgtcccct
      721 gcggccatga tggattccat ggctcttcgt tcgtgcacct gcgcctgcgg cgagggctgc
      781 tgccgctgtc tgtagagcgc tggcatccgc cccacggccc acggcagtct tctgcccacg
      841 ccagcgctcg ccccgactag gcccgttccg gcctagcccg ccccgacagg cgcccgcttc
      901 ggcgcacacc cacttcactc cccgcgatcg gcccggacgg gctgcgggac cccactcgac
      961 gaaggacatg acatggcaaa gatctacgac gacgcgaccc aactcatcgg tcataccccg
     1021 ctggtgcgca tcaacaagtt gaccaagggt tcgggagcga tcgtgctcgc gaagctcgag
     1081 ttctacaacc ccgcaagttc ggtcaaggat cgcatcggag tggcgatcat cgacgcggcc
     1141 gagaaggatg gctccctcaa gccgggcggg acgatcgtcg aagggacgag cggcaacacc
     1201 ggaatcgccc tggcgttcgt cggcgctgtc cgcggctaca aggtcgtcct cacgatgccc
     1261 gagaccatgt cgaaggagcg tcgggccctg ctccgcgcct tcggcgcgga gctgatcctg
     1321 acccccggtc ccgcgggcat gaagggcgcg gtcgaggagg cagagaggat cgcgaaggac
     1381 cgtcccggcg ccgtgcttgc gcggcagttc gcgaacctcg ccaaccccgc gattcaccgc
     1441 acgaccaccg ccgtggagat ctgggacgac accgacggca aggtggacat cctcatcgcc
     1501 ggcgtcggca cgggcggaac gatcacggga gtcggcgagg tcctcaaggc ccgcaagccg
     1561 agcgtccagg tcatcgcggt cgagcccgcg gagtctcccc tcctcaacgg cggcgagccc
     1621 ggcccccaca agatccaggg cctcggggcg aacttcatcc ctgagatcct caacacgaag
     1681 atctacgacg aggtgatcga cgtcgattcg gagaccgcga tcgccacggc gcgcgcggcg
     1741 gctcgcgagg agggcctcct ggtggggatc tcctcgggcg cggcgctcca cgcggcgtcg
     1801 atcgtcgcga agcggcccga gaacaagggc aagacgatcg tggtgatcat cccgtcgttc
     1861 ggcgagcgct acctctcgac cgtgctctac gcggacctca tggaatagtt gcaccacctt
     1921 ccgccggggg gcacggaagg aaaggggcgg cccgggcgtt actctggccc gggccgccca
     1981 ccaccccgaa catcgaggag tacatcgtga cgcagcacca gtcgcagcgg cacatagggc
     2041 cgctcgcgag gctcgtggag gacgtgcggg cggcgcacga ccgtgacccc gcggcccgct
     2101 cgaccctcgt catcgtcgcc tcctaccagg gcgtacacgc cctgtggtac caccgcgccg
     2161 cgcactggat gtggacgcac ggcatgcgca tccccggccg cctcctctcg cagcacg
//
LOCUS       DASVKP010000231         8317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_489919, whole genome shotgun sequence.
ACCESSION   DASVKP010000231 DASVKP010000000
VERSION     DASVKP010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8317
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489919"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VF362_04600"
     CDS             <1..433
                     /locus_tag="VF362_04600"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX7590241.1"
                     /translation="VANLFTTIAVPGAASSLLEYRFGQWGLDAATVAVHVPHYLSQSA
                     YPQATVRAMEALHTLSGLRFDPTALDEACASAAEEIDRQMSDSEDVQALVSALEAQYD
                     AFMESRDGVNPLGGILPTADEIGAEFEKFLADRGRDFPGLP"
     gene            586..789
                     /locus_tag="VF362_04605"
     CDS             586..789
                     /locus_tag="VF362_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX7590242.1"
                     /translation="MAQGAVKWFNAEKGFGFIAQDGGGQDVFVHYSAIETDGYRSLDE
                     NQRVEFEVTQGPKGPQAEKVRPL"
     gene            complement(961..1737)
                     /locus_tag="VF362_04610"
     CDS             complement(961..1737)
                     /locus_tag="VF362_04610"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX7590243.1"
                     /translation="MLRDRRTRGVAPATPRGEEVPIATGKAVLRDETDGSTTVLVNGV
                     ASSSLRADPSHLDFEYMRHAAAAIALWGPPAQMVVLHVGGAACTFPRHLAHHYPDSRH
                     LVVEVDPVLARLAREWGDLPRAPRLRIRVGDGLEVLRSRPDASVDVVVRDAFAGDATP
                     AHLADGAWWGEVRRVVRPGGLAIANVAAVPRSDDGRQDARFAGRAFASVCAIGEPAAL
                     AGTRMGNATIVAGDALDLDGLRRYAASAPLPTVVRVGWGT"
     gene            1736..2185
                     /locus_tag="VF362_04615"
     CDS             1736..2185
                     /locus_tag="VF362_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HEX7590244.1"
                     /translation="MSDTVALLGYQTPSGGLILESRQAAEEVAMPLSEYEQRVLDDLE
                     RDLGSDPRLKSAMTRSRGTVGRLVVAVVGVVVGLGVVLAGVMTKVWLIGIGGFALMIT
                     IVLWALLGPSASGRGTKRPAKGSAKSHQGFMTRLEERFERRREQGGV"
     gene            complement(2130..4337)
                     /locus_tag="VF362_04620"
     CDS             complement(2130..4337)
                     /locus_tag="VF362_04620"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEX7590245.1"
                     /translation="MTGTSRSPVAATPLLAVATFAAFWAFGAIVELGPWLERSALALA
                     GVTGVIMLVRLISRSRGLPTLAGAATTFLVLIPLYARGEDGTRFALPTPSAVSALFRT
                     FADGATYAQSAARPAAVTLEYESLLTLGVLVLFVAAEHLAVSWRAVATAGLVLLAPWA
                     PAVATRRSVPGVVVIVALAAWVLALAISRRSSPIGRRVPWTTSLLATAAVLVVVALVV
                     PTAIGGSGWGVIAERYAPDLFDGRNTRLDLDLDLRTSLTANSQTAVFAYVSPAGRADA
                     FRVYTFADFDGASWTYRDPAMSTVAAAGPVLWPTEVTDWATAPRAQLTVAMLSLTASN
                     LPIPVAPRTVDASGNWFYDAATDQIVGSQITTHDLTYSILVDFAYQNAEALRATDAEA
                     ALGQAEDLADPTYLAIPSAVALPRVLALTREVTADTTTRYDQALAIQSYLRDPTRFTY
                     DTSSSAAGSDAVSAFLDSGHGYCLQFATTMVMMLRSIGIPARLSEGFLPGTAASDGTF
                     VVRGADAHAWPEVYFAGRGWVRFEPTPAVQTGLPPAWADPDPNRVPGDATSGDVPAVP
                     AGPDDGTESPAIPTPIGDRTGGLPAWVIPLAAGAIAAAALSAFLWRRRGDRAHHGSPL
                     GAEAAWQRLNRRLREAGWPASATPMEATSHVLRAIRAANGRPVRAESVTALLSLSAAV
                     SDRRYAPSCEEVPQAQLSTWVEEVVAEADATTGKETKRRPARGGARNAPQAGS"
     gene            complement(4334..5641)
                     /locus_tag="VF362_04625"
     CDS             complement(4334..5641)
                     /locus_tag="VF362_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7590246.1"
                     /translation="MTPPSSPDASSTRRITSRGIGLAVAGLGIGALGIGLASPPLLYV
                     GVTAVAVVMVSSLWMLLSIHTFLLRFPFARREVAPLPLTVGAPGRVMVTIASAHALAP
                     RGGLHPGRRLSRSITDGLDIREQAAAELTGGAGTKATVARTAESLSLSYPLLPTRRGR
                     WPLGPAVVHSSDPFGLILADTCVGGAELLPVWPAIIDLTATAGALMGHADRVVLGART
                     PSPDDASLRDYREGDDLRRVHWKSSARRGEMLVRSDERAGRRPATVILDLPRESGPLE
                     WTISTGASIALSVLASGHPVRLLGGAIEPDGDDRSHSPALARVNLLNQTLDLQPPGSA
                     ASAERDLIRAGREAAVSAQSGDVTVGVFEPLSADALRALLTIGDHGRAWAIVRADGES
                     TQREAADATVKALRRAGWRATAVGVADDLSRMWTTLLTSGDLE"
     gene            complement(5638..6669)
                     /locus_tag="VF362_04630"
     CDS             complement(5638..6669)
                     /locus_tag="VF362_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7590247.1"
                     /translation="MNQPIPTEAELAEVAAVTARIRESISSVLAGLDPAIITTLAIVL
                     AEGHLLMEDVPGVGKTTLARALARSVDCTVGRIQFTPDLLPSDVTGVTIFRAGERDFE
                     FRPGPVFANVVIADEINRASPKTQSALLEAMQERAVTTDGVTRPLPSPFLVVATQNPV
                     EMEGTYPLPEAQRDRFMAQVAVGYPSARDEMVMLDTHDGTDPLRAIAPVTDIATLARL
                     VATTRRVYAAPAIKQYIVDLVGATRTESGLRLGASPRASLHLLAAAKARAAMGARNHV
                     LPDDVKALAVTVLAHRMILSTEARLAGRSARQVVTELVQRTPIPTEARDAAPPRAAAG
                     PSTGGLGRP"
     gene            6912..7355
                     /gene="mraZ"
                     /locus_tag="VF362_04635"
     CDS             6912..7355
                     /gene="mraZ"
                     /locus_tag="VF362_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX7590248.1"
                     /translation="MGPTGVFLGTFTPRLDDKGRLILPAKFRPRLATGLVCTRGLDRC
                     VFMFPVDEFHVVHDRLRRAPLENKRARDYLRSFLAYASDDLPDKQGRILLSAPLRAYA
                     GIDRDVAVVGLGSRVEIWDARAWEDYLARTEDDYAETAREVFGDI"
     gene            7492..>8317
                     /gene="rsmH"
                     /locus_tag="VF362_04640"
     CDS             7492..>8317
                     /gene="rsmH"
                     /locus_tag="VF362_04640"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX7590249.1"
                     /translation="MNDAVERHVPVMLDRCVELLRPALDSSGAIAIDATLGLGGHSEA
                     LLASSPGARLVGIDRDGDAIRLATARLGPFGARFAAHRAEYDDIAGALHAIGATRADA
                     ILFDLGVSSMQIDEADRGFSYAADAPLDMRMSRDDSRTAADLVREEDESTLARILAEY
                     GEERYAPRIARAIVRRRAIAPITRTGDLVEIVRGAIPAAARAKGGNPAKRTFQALRIA
                     VNDELGILARAIPAAIEATAVGGRIVVLAYHSLEDRLVKRAFAAGAEDQTPRLLPLT"
BASE COUNT         1227 a         2792 c         3104 g         1194 t
ORIGIN      
        1 cgttgccaat ctcttcacga cgatcgccgt gcctggagcg gcgtccagcc tgctcgagta
       61 tcgcttcggc cagtgggggc tcgacgccgc gactgtggcg gtccacgtgc cccactacct
      121 ctcccagtcg gcgtacccgc aggcgacggt gagggccatg gaggcgctcc acaccctctc
      181 gggcctgcga ttcgatccga ccgcgcttga cgaggcgtgt gcctccgcgg ccgaagagat
      241 cgataggcag atgagcgatt cggaggacgt gcaggcgctc gtatccgcgc tcgaagcgca
      301 gtatgacgcc ttcatggagt cccgcgacgg cgtcaatccg ttgggcggaa tccttccgac
      361 ggcggacgag atcggggcgg aattcgagaa gttcctcgcg gatcgtgggc gcgacttccc
      421 cggtttgccg taatccatgg cacacttatg gggttgcagt gatcgttagc gagacctggc
      481 tggatttccg gcgcggggcg tgggacgtag cattgcggca tttgacccgg caacgtgccg
      541 ggtcgccggg tccccggttg ggatgaaaag ttaggacagg taggcatggc tcagggagct
      601 gttaagtggt tcaacgccga aaagggcttt ggtttcattg cccaggacgg tgggggacag
      661 gacgtgttcg tccactactc cgccatcgag actgatggtt accgctcgct cgacgagaac
      721 cagcgggtcg agttcgaggt cacgcagggt ccgaagggtc cgcaggccga gaaggttcgt
      781 ccgctctaga ccgtcttccg acgcagtgtt ggtttccaac ccagcgtcgg ttccggccta
      841 gttacgcgaa cgtcacgagg gtcgttcccc gcaagggggg cggcccttgt cgcgtttccc
      901 cggtgctttc gcacgtgcca gcgccgtcgg acctgcagcg ccgtcggacc tgcagcgccg
      961 tcaggttccc cagcccaccc gcacgaccgt cggcaggggc gccgaggccg cgtatctgcg
     1021 caggccgtcg aggtccagcg catcgcccgc gacgatcgtc gcgttgccca tgcgagtgcc
     1081 cgcgagggcg gcgggctcgc cgatggcgca cacggacgcg aacgcgcgcc ccgcgaaccg
     1141 cgcgtcctgg cgaccgtcgt ccgacctcgg aaccgccgcg acgttcgcga tggcgagccc
     1201 gccgggcctc acgacccgcc tcacttcccc ccaccacgcc ccgtccgcta ggtgggcggg
     1261 tgtggcatcg cctgcgaagg cgtccctcac gaccacgtcg acgctcgcgt ccggcctgga
     1321 gcggagcacc tcgagcccgt cgccaacccg gatgcgcagg cgaggggctc ggggaaggtc
     1381 cccccactcc cgcgcgagtc gggcgaggac ggggtccacc tcgaccacga gatgccggga
     1441 atcggggtag tggtgcgcga ggtggcgcgg gaaggtgcag gcagcgccac ccacatgcag
     1501 gaccaccatc tgagcgggcg ggccccacaa cgcgatcgcg gccgcggcgt gccgcatgta
     1561 ctcgaaatcg aggtgggagg gatccgcccg gagcgacgaa ctcgcgacgc cgttcaccag
     1621 gacggtcgtc gagccgtcgg tctcgtcccg aagcaccgct ttcccggtcg cgatcgggac
     1681 ctcctcgccg cgcggcgtgg cgggtgcgac gcctcgggtc cggcggtcgc gtagcatgtc
     1741 cgacacagta gcgctcctgg gctaccagac cccgtccggg gggcttatcc tggaatcacg
     1801 gcaagccgcg gaggaggttg cgatgccgct ctcggagtac gagcagcgcg tgctcgacga
     1861 tcttgagcgc gatttggggt cggaccccag gctcaagagc gcgatgacgc gctcccgggg
     1921 tacggttggg cgcctcgtgg tcgccgtcgt cggcgtggtc gtcggccttg gcgtcgtcct
     1981 tgccggcgtg atgaccaagg tgtggctcat cggtatcggt ggcttcgcgc tgatgatcac
     2041 gatcgtgctg tgggcgcttc tgggcccctc cgcttccggc cgcggcacca agcgcccggc
     2101 caagggctcc gcaaagagcc atcagggttt catgacccgg cttgaggagc gtttcgagcg
     2161 ccgccgcgag cagggcggcg tttagtctcc ttgccggtcg tcgcgtccgc ctcggccacc
     2221 acctcttcga cccacgtgct cagttgcgcc tgcggaacct cctcacacga tggcgcgtac
     2281 cgccgatccg agaccgccgc gctgagtgag agaagcgcgg tcacggactc cgctcgcacc
     2341 ggacggccgt ttgccgcgcg gatcgcgcgg aggacgtgcg acgtcgcctc catgggagtg
     2401 gcgctcgctg gccacccggc ctcccgcagg cgacgattca gccgctgcca tgctgcctcg
     2461 gcgccgaggg gcgaaccgtg gtgcgctcga tccccgcgcc gccgccagag gaacgcggac
     2521 agcgcggcgg cggcgatcgc cccagctgcg agcgggatca cccacgccgg caggccaccc
     2581 gttcgatcgc cgatgggcgt ggggatcgcc ggagactccg tcccgtcgtc gggcccggcc
     2641 ggaaccgcgg gcacgtcgcc gctcgtcgcg tcgcccggca ccctgttggg gtccggatcc
     2701 gcccaagcag gcggcagccc ggtctggacg gcgggggtcg gctcgaatcg cacccagccg
     2761 cgacccgcga agtagacctc tggccatgcg tgggcgtcgg cgccgcgaac cacgaacgtg
     2821 ccgtcgctgg ccgccgttcc ggggaggaat ccctcgctga gtcgcgcggg aatgccgatc
     2881 gagcggagca tcatgaccat cgtcgtcgcg aactgcaggc agtagccgtg gccgctgtcg
     2941 aggaacgcgg agacggcatc gcttccggcg gcggacgacg aggtgtcgta ggtgaatcgg
     3001 gtggggtctc tcaggtagct ctggatcgcg agcgcctggt cgtaccgcgt cgtggtgtcc
     3061 gcggtaacct cgcgggtcaa cgccaggacg cgcgggaggg cgacggccga ggggatggcc
     3121 aggtaggtcg gatcggcgag gtcctccgcc tggccgaggg cggcctcggc gtccgtcgcc
     3181 cgcagggcct cggcgttctg gtacgcgaag tccacgagga tggagtaggt caggtcgtgc
     3241 gtggtgattt ggcttccgac gatctggtcg gtcgccgcgt cgtagaacca gttccctgac
     3301 gcatcgacgg tgcgaggcgc gacggggatc ggaaggttcg aggccgtgag ggagagcatc
     3361 gccaccgtga gttgggctcg gggcgccgtg gcccagtccg tcacctcggt gggccacagc
     3421 acggggcctg cggcggcgac ggtggacatc gcggggtcgc ggtaggtcca gctcgccccg
     3481 tcgaagtcgg caaacgtgta cacccggaag gcgtcggctc gcccggccgg cgagacgtag
     3541 gcgaagaccg cggtttggga gttcgcggtg agggacgtcc tgaggtcaag gtcaagatcg
     3601 aggcgcgtgt tccggccgtc gaacaggtcc ggggcataac gctcggcgat gacgccccag
     3661 cccgatcccc cgatggccgt cggaacgacg agcgcgacaa cgacgagcac ggccgcggtc
     3721 gccagaagcg atgtcgtcca tgggacccgt cgcccgatgg gggaacttcg ccgcgagatc
     3781 gcgagtgcga ggacccacgc ggcgagggcc acgatgacga ccacgcccgg aacggagcgt
     3841 cgcgtcgcga ccgcgggcgc ccacggcgcg agcaggacga gccccgcggt cgcgaccgcc
     3901 ctccaggaca cggcgaggtg ctcggcggcc acgaagagga cgagcacccc gagcgtgagg
     3961 agcgactcgt actccagggt cacggccgct gggcgggcgg cgctttgcgc gtaggtggcg
     4021 ccgtcggcga aggtccggaa gagcgcactc acggccgatg gggtggggag ggcgaaccga
     4081 gttccgtcct cgcctctcgc gtagaggggg atcagcacga ggaacgtcgt ggccgctccg
     4141 gcgagggtcg gaaggcctct cgaacgcgag atgaggcgga cgagcatgat cacgcccgtg
     4201 acgcccgcga gggcgagagc ggagcgctcg agccacgggc cgagctcgac gatcgcgccg
     4261 aacgcccaga acgcggcgaa ggtcgcgacg gcgaggaggg gggtcgcggc gacgggggag
     4321 cgcgaggtgc cggtcactcg aggtcccccg acgtcagaag cgtcgtccac atccgcgaca
     4381 ggtcgtcggc gacgcccacg gcggtcgcgc gccaccccgc gcggcggagg gccttgaccg
     4441 tcgcgtcggc ggcctcgcgc tgcgtcgatt cgccgtccgc cctcacgatc gcccaggctc
     4501 ggccgtgatc gccgatcgtg aggagggcgc gcagcgcatc cgccgacagg ggctcgaaca
     4561 ctcccaccgt gacgtcgccc gactgcgccg agacggcggc ctcgcggcca gcgcgaatga
     4621 ggtccctctc ggccgatgcc gcggatccgg gcggctgaag gtcaagggtc tggttgagca
     4681 ggttgaccct ggcgagggcg ggggagtggg agcggtcgtc cccgtccggc tcgatcgccc
     4741 cgccgagaag gcggacgggg tggccggagg cgagcacgga cagggcgatg gaggcgcccg
     4801 tcgagatcgt ccactcgagc gggccgctct cgcgcgggag gtcaaggatt acggtcgcgg
     4861 gccttcggcc cgcgcgttcg tccgagcgaa cgagcatctc gccccgcctc gccgagctct
     4921 tccagtgcac gcgcctcagg tcgtccccct cgcggtagtc gcgcagcgag gcgtcgtccg
     4981 gcgaaggcgt gcgcgcgcca aggaccaccc gatcggcgtg acccatgagc gcgcccgcgg
     5041 tcgcggtcag gtcgatgatg gccggccaca cgggaaggag ttccgcgccc ccgacgcagg
     5101 tatcggcgag gatcagcccg aacggatcgc tgctgtggac gacggcgggg ccgagcggcc
     5161 atcgcccccg cctcgtggga aggagcgggt aggagaggga gagcgactct gcggtccggg
     5221 cgaccgtcgc cttggtgccc gccccgccgg tgagttccgc cgcggcctgc tcacggatgt
     5281 cgaggccgtc ggtgatgctg cgcgagagcc gcctgccggg gtgaagccca ccccggggag
     5341 ccagcgcgtg cgccgacgcg atggtgacca tcaccctgcc cggcgctccg acggtcaggg
     5401 gaagcggcgc gacctccctc cgcgcgaagg ggaagcgcag caggaaggtg tggatggaca
     5461 ggagcatcca gagggaggag accatcacga cggcgacggc ggtgacgccc acgtagagga
     5521 gcggcgggct cgcgaggccg atgccgagcg ccccgatccc gagccccgcg accgcgagac
     5581 cgattccgcg cgacgtgata cgtctcgtcg agctcgcgtc gggcgaggac ggcggcgtca
     5641 tgggcggccg aggcctccgg ttgagggtcc ggcggcggca cggggcgggg cggcatcgcg
     5701 cgcctccgtc gggatcggag tcctctggac gagttccgtg acgacctgac gcgcgctcct
     5761 gcccgcgagc cgcgcctcgg tgctgaggat catgcggtgg gcgaggacgg tgacggcgag
     5821 tgccttcacg tcatcgggga gcacgtggtt ccgcgcgccc atcgccgcgc gggccttcgc
     5881 ggcggcgagg aggtggaggg atgcgcgcgg cgacgccccg agccggaggc ctgactccgt
     5941 cctggtggcc cccacgaggt cgacgatgta ctgcttgatc gccggtgcgg cgtagacgcg
     6001 cctggtcgtg gcgacgaggc gcgcgagggt cgcgatatcg gtcacggggg caatggcgcg
     6061 caggggatcg gtgccgtcgt gggtgtccag catcaccatc tcgtcccgcg cggaggggta
     6121 ccccaccgcg acctgcgcca tgaagcgatc gcgctgggcc tcgggcaggg ggtaggtccc
     6181 ttccatctcg acggggttct gggtcgcgac gacgaggaac ggagagggga gcggccgcgt
     6241 cactccatcg gtcgtgacgg cgcgctcctg catggcctcc aggagcgcgg actgggtctt
     6301 gggggacgcc cggttgattt cgtcggcgat gacgacgttg gcgaagaccg gtccgggccg
     6361 gaactcgaag tcgcgctctc ccgcgcggaa gatcgtcacc ccggtcacgt cggacgggag
     6421 gaggtccggg gtgaactgga tgcgccccac cgtgcagtcc accgaccggg cgagcgcccg
     6481 cgcgagcgtc gtcttgccga cgccggggac gtcctccatg agcaggtggc cctcggcaag
     6541 gacgatcgcg agcgtcgtga tgatcgccgg atcgagtccc gcgagcacgg acgagatcga
     6601 ctcgcgaatg cgggcggtga ccgcagcgac ctcggcgagt tcggcctcgg tgggaatcgg
     6661 ttggttcatg gacgctccct ttgcctcgtg tctccgagcc taggtgatgg ggggtcgtgg
     6721 gcgcgccctc caccccctat gtcgggccgc taccagggac gacgaccctt gcggcgggcg
     6781 cggccacggg aatcttgcca gaagtggagg gaagtggagt aaagtggggc gcgatggtgt
     6841 ggaaggggcg tgccgtaggc ctgccccgcg gcgaggagga gccggtcgtg tcggacgttg
     6901 ccgtaaacgg gatgggcccc acgggggtct ttcttggcac gttcacgccc cgtctcgacg
     6961 acaaggggcg cctcatcctt cccgcgaagt tccggccacg cctcgccacg ggcctggtct
     7021 gcacccgcgg gctcgatcgc tgcgtcttca tgttccccgt ggacgagttc cacgtcgtcc
     7081 acgaccggct gcgccgggct ccgctcgaaa acaagcgggc gcgcgactac ctcaggagct
     7141 tcctcgccta cgcgagcgac gaccttcccg acaagcaggg acgaatcctc ctgtccgctc
     7201 cgctccgggc ctacgccggc atcgaccgcg acgtcgcggt cgtggggctg ggctcgcggg
     7261 tggagatctg ggacgcgcgg gcatgggagg actacctcgc ccgcacggaa gacgactacg
     7321 ccgagaccgc gagggaggtc ttcggggaca tctagacgtg agacgccatc gtgccgtgcg
     7381 gcctctctcc cgcgcgaact ctggggcact tccccgcttc agagccgccg gagggagacc
     7441 gggcgacacg acggagaggt caccgcgccg gatggaggag aggaggggag cgtgaacgac
     7501 gccgtcgaac ggcacgttcc cgtgatgctc gaccgttgcg tcgagcttct tcgccctgcg
     7561 ctggactcct cgggagcgat cgcgattgac gcgacgctcg gcctcggtgg ccactcggag
     7621 gcgcttctcg cgtcgagccc cggcgcgcgc ctcgtgggca tcgatcgcga cggtgacgcg
     7681 attcggctcg cgaccgcgcg tctcgggccc ttcggggcac gcttcgcggc gcatcgcgcc
     7741 gagtacgacg acatcgccgg agccctgcat gccatcggcg cgacgagggc ggacgcgatc
     7801 ctgttcgacc tcggggtgtc gtcgatgcag atcgacgagg ccgatcgcgg cttcagctac
     7861 gcggccgacg ccccgctcga catgcggatg agcagggatg actcccggac ggcggcggat
     7921 ctggtgcgcg aggaggacga gtcaacgctc gcgaggatcc ttgccgaata cggcgaggag
     7981 cggtacgccc ctaggatcgc tcgcgccatc gtgcgcaggc gcgcgatcgc acccatcacc
     8041 cgcacgggcg acctcgtcga gatcgtgcga ggcgcgattc ccgcggccgc cagggccaag
     8101 gggggcaatc ccgccaagcg gaccttccag gccctcagga tcgccgtcaa cgacgagctc
     8161 gggatcctcg cgcgggcgat ccccgcggcc atcgaggcga ccgccgtcgg gggtcggatc
     8221 gtcgtgctcg cataccactc gctcgaggac cggctggtca agcgcgcgtt cgcagcggga
     8281 gcggaggacc agacgcccag gcttcttccc ctcacgc
//
LOCUS       DASVKP010000232         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1148070, whole genome shotgun sequence.
ACCESSION   DASVKP010000232 DASVKP010000000
VERSION     DASVKP010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148070"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..553)
                     /locus_tag="VF362_04645"
     CDS             complement(167..553)
                     /locus_tag="VF362_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7590250.1"
                     /translation="MSIDSRTPEVEATSHHLSPQDQAAVDALPIGSALLIVHHGPNAG
                     ARFLLDRDLTTAGRSVDGDIFLDDVTVSREHVRFERRGGAYYAKDAGSLNGTYVNYER
                     IVTRKLEAGDEVHIGKYRLTFYPGPA"
     gene            complement(677..1240)
                     /locus_tag="VF362_04650"
     CDS             complement(677..1240)
                     /locus_tag="VF362_04650"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX7590251.1"
                     /translation="MSENREPTSRVWTIPNIISMLRIVLIVAFAVLLARHADGWAIIA
                     LVAAGVSDFFDGFLARRWNQVTTLGRVLDPAADRLLTLAVVIGLGLRGIIPWWLVGVL
                     LARDAVVGIALLIGRRTGVGTPQVTFIGKAATFGLYWFLPLSYLAYGHSDVWHAIGIW
                     GGAASAAVYWYAGLGYVRDVARRSRAA"
     gene            complement(1281..1706)
                     /locus_tag="VF362_04655"
     CDS             complement(1281..1706)
                     /locus_tag="VF362_04655"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX7590252.1"
                     /translation="MTATIPTVLFVCVHNAGRSQMAAGWLEALGSGRVRVLSAGSMPA
                     NRVNPVAVEAMAEVGIDIGSNQPKVLTDGAVQESDVVVTMGCGDACPFYPGKRYEDWK
                     LDDPAGQNLEAVRPIRDKIRERIEALLESLGVTPSSDPA"
     gene            complement(1777..2439)
                     /locus_tag="VF362_04660"
     CDS             complement(1777..2439)
                     /locus_tag="VF362_04660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7590253.1"
                     /translation="MTDEPLRSSAERWDRKYSERGALWSGRANPVLREVAARLEPGTA
                     LDVGCGEGGDAVWLAEQGWTVVGIDLSAVALERAAAAAAERGVGERCTWIAGDVTQGV
                     VASGIGTGIGTDTGFDLVTSHYLHEPPEVRSAAWLAEADLTAPGGVLLIVGHHPEDEA
                     PAGRGPRDPSVLFSPDEVAGALSAVRGLAVETSEMRERLAEGPDGPIPRRDTVVVARR
                     TS"
     gene            complement(2436..>3030)
                     /locus_tag="VF362_04665"
     CDS             complement(2436..>3030)
                     /locus_tag="VF362_04665"
                     /inference="COORDINATES: protein motif:HMM:NF037864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX7590254.1"
                     /translation="ARALGISWGAAESYADCLAHLDLATPAAAAFLSEATTLFRGARW
                     EPFDDSDPSLPRPASMTHGALGVPYAHVTAPLRRLADRYATEICLAHAGGVSVPAWVQ
                     RALAQIGDEMERGTHIARSVDHDCISAVESAVLAPYVGTDFQGVGLDDRTVQLAEPAV
                     VARCEGGVEAGKDQTVRLVSADPDRGPVFAVETGGGA"
BASE COUNT          449 a         1059 c         1108 g          414 t
ORIGIN      
        1 gcgggaaact cgggctggac aatcgcgagg acgtcggaga gccgcatgag cggatcgtga
       61 tacagcgcgt ggggccaacg ccccgtcgaa gcgcgttcct gttccgctgg ggtgccgacg
      121 gccgctcggt caggcagggg ggcggcggcg agggccgacg gcatgcctag gcggggccgg
      181 ggtagaaggt gagtcggtac ttgccgatgt gcacctcgtc gcccgcctca agcttgcgcg
      241 tcacgatgcg ctcgtagttg acgtaggtcc cgttgagcga gcccgcgtcc ttcgcgtagt
      301 aggcgccgcc acgtcgctcg aagcgaacgt gctcgcgcga gacggtcaca tcgtcaagga
      361 agatgtcgcc gtcgacgcta cgacccgcgg tcgtgagatc cctgtcgagg aggaagcgcg
      421 cgcccgcgtt cggcccgtga tgcacgatga gcagcgcgga gccgatcggg agcgcgtcaa
      481 cggcggcctg gtcctgtggg ctcaggtggt gggacgtcgc ctccacctcg ggggtgcgcg
      541 aatcgatgga catcgtttgc tctgcgggat tctcgtcgtc catgttcgct cccctcgggt
      601 cggtcgttgt gcccatcgta gtccggtcgc ggcggcgcgt cctaggagag cggcgtccta
      661 ggcgggccgc cgcgacctat gcggcgcgcg aacgcctcgc cacgtcgcgc acgtacccga
      721 gcccggcgta ccagtagacc gctgcagacg cggcgccacc ccagatgccg atcgcgtgcc
      781 acacgtccga gtggccgtat gcgaggtagc tgagcggcag gaaccagtac aggccgaagg
      841 tcgcggcctt cccgatgaac gtgacctgag gcgtccccac acccgtgcgc cttccgatca
      901 ggagggcgat cccgacgacg gcgtcacgcg cgagcagcac cccgacgagc caccacggga
      961 tgattcccct caggccgagc ccgatgacga ccgcgagggt gaggagcctg tcggcggcgg
     1021 gatcgaggac ccgcccgagc gtcgtgacct ggttccaccg gcgcgcaagg aaaccgtcga
     1081 agaagtcgga gaccccggcc gcgacgaggg cgatgatcgc ccagccgtcc gcgtgacgcg
     1141 cgaggaggac ggcgaacgcg acgatcagga cgatgcgcag catcgagatg atgttgggga
     1201 tggtccagac gcgcgacgtg ggttcccgat tctcgctcac tgctccactc tagggcgagc
     1261 cggggcgcgc gcggcgcgcc ctacgcgggg tcggacgagg gggtgacgcc gagcgactcg
     1321 aggagcgcct cgatccgctc gcggatcttg tcgcggatgg ggcgcaccgc ctcgaggttc
     1381 tggccagcgg ggtcgtcgag cttccagtcc tcgtagcgct tgccggggta gaacgggcat
     1441 gcgtcgccgc agcccatggt gacgacgacg tcggattcct gcaccgcgcc gtccgtcagc
     1501 accttcggct ggttcgagcc gatgtcgatc ccgacctccg ccatcgcctc gacggccacg
     1561 gggttgaccc ggttcgcggg catggagccc gcggacagga cccgaacgcg tcccgacccg
     1621 agggcctcga gccagccagc ggccatctgc gagcggccgg cgttgtggac gcacacgaag
     1681 aggacggtgg ggatcgttgc ggtcatggga gcaagattac gtccccgccg cgaagcaggg
     1741 atgggcgttc acccaaggtt caccttgggc acggcttcac gacgtccggc gcgccaccac
     1801 cacggtgtcc ctgcgcggga tcggcccgtc ggggccttcc gcgagtcgct cgcgcatctc
     1861 gctcgtctcc acagcgagcc cccgcacggc ggagagcgcc ccggcgactt cgtcgggaga
     1921 gaacagcacc gagggatccc gcggcccgcg gcccgcaggg gcctcgtcct cggggtgatg
     1981 gccgacgatg aggaggacgc cgccgggggc cgtcaggtcc gcctcggcga gccaggccgc
     2041 ggagcggacc tccggcggct cgtggaggta gtggctagtc accaggtcga agccggtgtc
     2101 ggtgccgata ccggtgccga tgccggaggc gacgaccccc tgcgtgacgt cccccgcgat
     2161 ccaggtgcac cgctccccca ctccccgctc ggccgcggcc gcagccgcgc gctcgagggc
     2221 gacggccgac aggtcgatcc ccacgaccgt ccacccctgt tcggcgagcc agacggcgtc
     2281 gccaccctcg ccgcaaccaa cgtcgagcgc ggtgccgggt tcgagccgcg cggcaacctc
     2341 gcgaagcacg gggttggcgc ggccgctcca gagggctccg cgctccgagt acttcctgtc
     2401 ccagcgctcc gcgctggacc tcaggggttc gtccgtcacg ccccgccccc ggtctcgacg
     2461 gcaaagaccg gtccgcggtc cgggtcggcg ctaaccaggc gcaccgtctg gtccttgccc
     2521 gcctcgactc ccccctcgca ccgcgcgacc acggcgggct ccgcgagctg gacggtccga
     2581 tcgtcgaggc cgacgccctg gaagtccgtc ccgacgtacg gcgcgaggac cgcggattcc
     2641 acggcggaaa tacagtcgtg atcgacggac cgcgcgatgt gggttccgcg ttccatctca
     2701 tccccgatct gcgccagcgc gcgctgaacc cacgcgggaa cggagactcc gcccgcgtgg
     2761 gcgaggcaga tctcggtcgc gtaccggtcc gcgaggcggc ggagcggcgc ggtcacgtgc
     2821 gcgtacggga cgccgagcgc cccgtgcgtc atggaggccg gtcgcggaag gctcgggtcg
     2881 gagtcatcga agggttccca gcgcgcgccg cggaagaggg tcgtcgcctc ggaaaggaag
     2941 gccgccgcgg cgggggtcgc gaggtcgagg tgggcgaggc agtccgcgta ggactcggcc
     3001 gcaccccagg agatcccgag cgcgcgcgcc
//
LOCUS       DASVKP010000233         2464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1475378, whole genome shotgun sequence.
ACCESSION   DASVKP010000233 DASVKP010000000
VERSION     DASVKP010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475378"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VF362_04670"
     CDS             <1..1018
                     /locus_tag="VF362_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HEX7590255.1"
                     /translation="TELLLRLYKNLVLGDSSQPVPQFTSCSPGWVKYIEHKYPEYLPN
                     MSTCKSPQQMFGALIKTWYADKSGIDPADVVSVSLMPCSAKKFEVSRPEMTDSGYPDV
                     DYALTTREMGKMIKEHGLNLPDLPRGEFDDPFGTATGSGVIFGATGGVMEAALRTVIE
                     LVTGLKAEAVFAHADILPVRGFEGAKYVEVTIPDEVGPVPAILAHLVPDWNWLKGATL
                     KVAVVHGTANAEKVMEDIKAGGKFSECHFIEFMACPGGCIGGGGQPIPTSADIRAARA
                     RAIYSEDSAYAVRKSHENQAVLNLYGEFLTEGPGGHTAHHLLHTDYTARGTFIATGDN
                     AKGE"
     gene            1021..1524
                     /locus_tag="VF362_04675"
     CDS             1021..1524
                     /locus_tag="VF362_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX7590256.1"
                     /translation="MLNVPVALATKQSDLIDNIDALIEEHGGGRDALIPVLQDLRKKR
                     HDISDLAMQVIADRLGLSPVEVQGVATFYSFLQVGATGQHTIRVCRTLSCSFAGATSI
                     AQKLEAELGISMGETTADGQVTLEWANCIGMCDQAPALLADQAAVGSLTTARVGEIVA
                     NLKASKA"
     gene            1624..1809
                     /locus_tag="VF362_04680"
     CDS             1624..1809
                     /locus_tag="VF362_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590257.1"
                     /translation="MSNDNRKAAGASGGAVYGLGLIGAAVYYFQQASDLWSYVFALPK
                     AIVWPAFVIYKLLKHLA"
     gene            complement(1968..>2464)
                     /locus_tag="VF362_04685"
     CDS             complement(1968..>2464)
                     /locus_tag="VF362_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590258.1"
                     /translation="QLRAQMVAQLNRLASDAAAADGHLFAMSTLVADGIAIPMSLTVF
                     RPRFLGGLRDVTDSLRGQPDVVVAQGVTGDVIRRVATVEPRTRTDATPAGTSEFSETP
                     VAMLKVDYWLDPADGVGLFHVVYTSPLVEAREQLLELFDAITSTVEAPAEGDVEARLP
                     DESA"
BASE COUNT          426 a          836 c          831 g          371 t
ORIGIN      
        1 caccgagctc ctccttcgcc tgtacaagaa cctggtcctc ggcgattcga gccagcccgt
       61 cccgcagttc acctcatgca gccccggctg ggtgaagtac atcgagcaca agtaccccga
      121 gtacctgccg aacatgtcca cgtgcaagag cccccagcag atgttcgggg ccctcatcaa
      181 gacctggtac gccgacaaga gcggcatcga ccccgcggac gtcgtctcgg tctcgctcat
      241 gccgtgctcg gccaagaagt tcgaggtctc gcggccggag atgaccgaca gcggctaccc
      301 cgatgtcgac tacgccctca ccactcgtga gatgggcaag atgatcaagg agcacgggct
      361 caacctgccc gacctcccgc gcggcgagtt cgacgacccg tttgggacgg cgaccggctc
      421 cggcgtcatc ttcggcgcga ccggcggcgt catggaggcg gccctccgca ccgtgattga
      481 actcgtgact gggctcaagg ccgaggccgt cttcgcgcat gccgacatcc ttcccgttcg
      541 cgggttcgag ggcgcgaagt acgtcgaggt cacgatcccg gatgaggtcg gtccggtgcc
      601 cgcgatcctc gcgcacctcg tccccgactg gaactggctc aagggcgcga ccctcaaggt
      661 cgcggtcgtc cacggcacgg caaacgccga aaaggtcatg gaagacatca aggccggcgg
      721 caagttcagc gaatgccact tcatcgagtt catggcgtgc cccggtggat gcatcggggg
      781 cggcggccag ccgatcccga cgagcgcgga catccgcgcg gcgcgggcac gggcgatcta
      841 ctccgaggac tcggcctacg cggtgcgcaa gagccacgag aaccaggcgg ttctgaacct
      901 ctacggcgag ttcctcacgg agggcccggg cggacacacc gcccaccacc ttctccacac
      961 cgactacacc gcacgcggga cgttcatcgc gaccggcgac aacgcgaaag gcgagtagac
     1021 atgctgaacg taccggtggc gcttgcgacc aagcagtcgg acctgatcga caacatcgac
     1081 gcactgatcg aggagcacgg gggcgggcgc gacgccctca tccctgtcct ccaggacctg
     1141 cgcaagaagc gccacgacat cagcgatctc gcgatgcagg tgatcgccga ccggctcggc
     1201 ctctccccgg tcgaggtgca gggcgtcgca accttctact cgttcctcca ggtcggcgcg
     1261 acgggccagc acaccattcg ggtgtgccgg acgctcagtt gctccttcgc gggcgccacg
     1321 agcatcgcgc agaaacttga ggccgaactc ggtatctcca tgggcgagac cacggccgac
     1381 ggccaggtca cgctcgagtg ggcgaactgc atcggcatgt gcgaccaggc gcccgcgctc
     1441 ctggccgacc aggccgcggt cggaagcctc acgacggccc gggtcggcga gatcgtcgcc
     1501 aacctcaagg cctccaaggc ctagccctac cggcgcccgg ggcggccttg ctcgccgccg
     1561 atggggccaa taggctgggt cggccgcgat ggtcgcggcg gatggagacc ccgggggtgt
     1621 ggagtgtcga acgacaaccg caaggcggcg ggcgcgagcg gaggggccgt ctatggccta
     1681 gggctcattg gcgccgccgt gtactacttc cagcaggcga gcgacctctg gagctacgtc
     1741 ttcgcgctcc ccaaggcgat cgtctggccc gcgttcgtga tctataagtt gctgaagcac
     1801 ctcgcgtaga gcgaaatggc ggcgcggcac cctggcggtg cttcggtgcc ggatcgtcgc
     1861 cgcaggcggc actggaccac cgcaggggtg cggtggggtg gtgtgtcggc gaggctgacc
     1921 cgggacgtcg gccgcgagcc catcggccgt cgcgccgttc gcacggctca cgcggactcg
     1981 tcgggaagcc tcgcttcgac atcgccctcg gcgggggcct cgaccgttga ggtgatggcg
     2041 tcgaagagtt cgagcagttg ctccctggcc tccacaaggg gcgacgtgta caccacgtgg
     2101 aagagtccga cgccgtccgc gggatcaagc cagtagtcga ccttcagcat cgccacgggc
     2161 gtttcggaga attccgacgt ccccgcgggc gtcgcgtcgg tgcgggtgcg gggctccacc
     2221 gtcgccacgc gccgaatgac gtcgccggtc acaccctgtg cgacaacaac gtcaggttgc
     2281 ccgcggaggc tgtcggtcac gtctctgagc ccgccgagga agcgaggccg gaagaccgtg
     2341 agcgacatcg gaatcgcgat accgtctgcg acgagggtgg acatggcaaa gaggtgaccg
     2401 tcggcggcgg ccgcatcgct ggcgagacga ttcagttgag ccaccatctg cgcgcgcagt
     2461 tggg
//
LOCUS       DASVKP010000234         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1149349, whole genome shotgun sequence.
ACCESSION   DASVKP010000234 DASVKP010000000
VERSION     DASVKP010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149349"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="VF362_04690"
     CDS             complement(<1..1524)
                     /locus_tag="VF362_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX7590259.1"
                     /translation="MNPPAPNQGLEGVQGAGGVLGYIVGTGLSKLVTAPFAVVLLVLA
                     GLSGVLVATRTPVHDVWPRVRGLWRGDATDEALDMPEHRTLAQRGKGDRRDHAGRRAK
                     PAHASDELDSYVGDEAFARTLGSEFDGIIHPGGGPAFAGGPTWRVDPNPRHVAGDDDS
                     PTAPLGHAMGAGGRVSPYALDGPPDHLGPQAHPGPPAATHLIPRGEQGELEDSGPYVL
                     PGLDVLVKGAPHKTRSQANDRVVEALQEVFDQFDVDADVVGFMRGPTVTRYEVELASG
                     VKVEKITQLYKNIAYAVASPDIRILSPIPGKSAIGIEIPNVDRETVVLGDVLRSPIAQ
                     KMDHPMVIALGKDVEGGYVMANLAKMPHLLVAGATGSGKSSFINAMIMSVLMRSSPRE
                     VRMVLVDPKRVELTAYEGIPHLITPIITDPKKAAQALEWVVKEMDVRYDDLAQFGFKH
                     IDDFNKAVRTGALKPLPGSNRKLAIYPYLLVIVDELADPMMVAPRDVDGAIQRITQLA
                     "
BASE COUNT          216 a          517 c          555 g          254 t
ORIGIN      
        1 cgcaagctgg gtgatgcgct ggatcgcgcc gtcgacgtcg cgcggcgcca ccatcatggg
       61 gtccgcgagt tcgtcgacga tcacgagcag gtacgggtag atcgcgagtt tcctgttgga
      121 gccgggcagc ggcttgagcg cgccggtccg cacggccttg ttgaagtcgt cgatgtgctt
      181 gaagccgaac tgggcgaggt cgtcgtagcg gacgtccatc tccttgacga cccactccag
      241 ggcctgcgcc gccttcttgg ggtccgtgat gatgggcgtg atgaggtggg gaatgccttc
      301 gtacgcggtg agctcgacac gcttggggtc cacgagcacc atccggacct cgcgcgggga
      361 tgagcgcatc aggacggaca tgatcatcgc gttgatgaag ctcgacttcc ccgaacccgt
      421 cgcccccgcc acgaggaggt gcggcatctt cgcgaggttg gccatcacat agccgccctc
      481 gacgtctttg ccgagcgcga tgaccatcgg gtggtccatc ttctgggcga tgggcgatcg
      541 cagcacgtcc cccagcacta cggtctcgcg atcgacgttg gggatctcga tcccgatcgc
      601 gctcttgccg gggatgggac tcaggatccg gatgtccggg ctcgcgacgg cgtaggcgat
      661 gttcttgtag agctgggtaa tcttctccac cttgacgccc gaggcgagct cgacctcgta
      721 ccgggtgacc gtcggtcccc gcatgaagcc caccacgtcc gcgtcgacgt cgaattggtc
      781 gaacacctcc tgaagcgcct cgaccacgcg gtcgttcgcc tgggagcggg tcttgtgggg
      841 cgcgcccttg acgaggacgt cgaggccggg gagcacgtag ggaccggagt cctcgagttc
      901 cccctgctcc ccgcgcggaa tcaggtgcgt cgcggccggg gggccggggt gggcctgcgg
      961 gccgaggtgg tcgggcgggc cgtcgagcgc gtacggggag acgcgcccgc ccgcgcccat
     1021 ggcgtggccc agtggcgcgg tcggcgagtc gtcgtcgccc gccacgtgac gcgggttcgg
     1081 gtccacgcgc caggtgggac cccccgcgaa ggcgggcccg ccgcccgggt ggatgattcc
     1141 gtcgaactcg ctcccgaggg tgcgggcgaa cgcctcgtcg cccacgtagg aatcgagttc
     1201 gtccgacgcg tgggccggct tcgcgcggcg gccggcgtgg tcgcgccggt cgcccttgcc
     1261 cctctgcgcg agggtgcgat gctcgggcat gtcgagcgcc tcgtctgtcg cgtcgccacg
     1321 ccatagaccc ctcacccgcg gccagacgtc gtgcaccggc gtccgggtgg cgaccaacac
     1381 gcctgagagc cccgcgagga cgaggagcac gacggcgaag ggcgccgtga cgagcttcga
     1441 aaggccggta ccgacgatgt agcccaggac tccccccgcg ccctggacgc cctcaagccc
     1501 ctggttgggc gcgggcggat tcaccccgat atggatgatc cc
//
LOCUS       DASVKP010000235         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1149934, whole genome shotgun sequence.
ACCESSION   DASVKP010000235 DASVKP010000000
VERSION     DASVKP010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149934"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1699)
                     /locus_tag="VF362_04695"
     CDS             complement(<1..1699)
                     /locus_tag="VF362_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590260.1"
                     /translation="MTASTPPGTPALSRGRRLAALARSALAAAPERLTLALVSLAIAC
                     GVAVSLAAFRPATVLPLAAVLTTIGWVLGPKGQRDPRSALGGALALGGAVLWFLANLR
                     WVAEYLIVRRDPGFLTLSALWLTDHPSTDIPTRGAIAAADGLPDVLAEAHEAWNLNGD
                     MLQPQGAKMLPALAAVGGWIHGESGVLAANLAIGAIGLLAVYVLARRMMGPIAALVPA
                     LGLSLTVSHIWLSRANYTEPITMVLLVVALTWAWHGVEEGRIFPLVAAGVASGSTYLG
                     RVDGPLFSLGVAAGVAVALAVSRTGSLRWRSAAFVSFAGAQAALTLAGNASLWRWSRA
                     YVERLGPESGDLLRAYALILGALVLVAITAPVVSALRQFTALRQSTAPRQSTALHQST
                     ALRNRTALSTGSARSKERRLGRIARTRLPGAMAWSTLLLLLALASRPLWMTAHTAPDD
                     FQVAFVAWIQQSQGLAVDGTRTYAENTITWVSYYLTWPVVVLGFAGFALAARRLGRGT
                     AVWAIPLAGLLVPSLLYVVRPAIMPDQSWAIRRLAPSLVVGLLLLAAVAWQAVIARWS
                     "
     gene            1791..2471
                     /locus_tag="VF362_04700"
     CDS             1791..2471
                     /locus_tag="VF362_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7590261.1"
                     /translation="MATIVLVEDDPTIAEPLTRALAREGYTVEAYATGIGGVEASERA
                     DLMILDLGLPDIDGIEVARRVRDKGLTVPILMLTARVDEVDLVVGLDAGADDYVTKPF
                     RLAELLARVRALLRRRVSADGAAELEVNGVRIDVAGHRAFADGRELQLSGKEFDLLHA
                     LIANAGTVVSRDTLMRDVWGADAGAPSKTLDMHVSWLRRKLGDDANDPRFITTVRGLG
                     FRFETGVE"
     gene            2481..>2727
                     /locus_tag="VF362_04705"
     CDS             2481..>2727
                     /locus_tag="VF362_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7590262.1"
                     /translation="MRRRLLYATISAVLAAVVLLGVPLAVAAAQSVRDGSRTELDARA
                     VTVARALEARYINGETISDAILESYVGGKNSPAAYVLV"
BASE COUNT          468 a          891 c         1014 g          354 t
ORIGIN      
        1 cggaccaccg cgcgatcacc gcctgccagg cgaccgccgc gagcagcagg aggccgacga
       61 cgagagacgg cgcgagtcga cggatcgccc acgactgatc gggcatgatc gcggggcgca
      121 cgacgtacag cagcgacggg acgagcagtc ccgccagggg aatcgcccac acggccgttc
      181 cgcgcccgag cctccttgcc gcgagggcga agccggcgaa tcccagcaca accacgggcc
      241 acgtcaggta gtagctcacc cacgtgatcg tgttctcggc gtacgtgcgg gtgccgtcga
      301 cggcaagacc ctgcgactgc tgtatccagg cgacgaacgc gacctggaag tcgtccggtg
      361 ccgtgtgggc ggtcatccag aggggacgag aagcgagggc aagcaggaga aggagggtgg
      421 accaggccat cgcccccgga aggcgggtcc tcgcgatccg gccgagccgg cgctccttgc
      481 tccgcgcgga tccggtcgac agcgccgtgc ggttccgcag tgccgtggat tggtgcagtg
      541 ccgtggattg gcgcggtgcc gtggattggc gcagtgccgt gaattggcgc agtgccgaga
      601 cgacgggcgc agttatggca acgagcacaa gcgcgccaag gatgagcgcg taggcgcgca
      661 gcaggtcgcc cgactcggga cccaggcgct cgacgtacgc gcgggaccag cgccacaacg
      721 aggcgttacc tgcgagggtg agggccgcct gggcgccggc gaacgagacg aaggccgcgg
      781 aacgccaccg cagcgatccg gtgcgggaga cggcgagcgc gaccgcaact ccggcggcga
      841 cgccgaggga gaagaggggg ccatcgacgc gcccgagata ggtcgatccg ctcgcgaccc
      901 cggccgcgac gagggggaag atccgcccct cctcgacccc atgccaggcc catgtgagcg
      961 caactaccag aagcaccatc gtgatgggtt cggtgtaatt ggcccgcgag agccagatgt
     1021 gcgagacggt gagcgacagc ccgagcgcgg gaacgagcgc cgcgatcggg cccatcatgc
     1081 gccgggcgag gacgtagacg gcaaggaggc cgatcgcgcc gatcgcaagg ttggcggcga
     1141 ggacgccgga ctcgccgtga atccatcccc cgacggccgc gagggccggg agcatcttcg
     1201 cgccctgggg ctggagcatg tcgccgttga gattccatgc ctcgtgggcc tcggcgagga
     1261 cgtccggaag cccatccgcg gccgcaatcg cgccgcgcgt ggggatgtcc gtcgaggggt
     1321 gatcggtgag ccagagcgcg ctcagcgtga ggaacccagg atcgcgtctc acgatgaggt
     1381 actccgcgac ccacctgagg ttcgcaagga accacaagac cgctcccccc agggcgaggg
     1441 cgccgccaag ggcggaccgg ggatcgcgct ggcccttcgg ccccaggacc cacccgatcg
     1501 tcgtgaggac ggcggcgaga gggagcacgg tggcggggcg gaacgcggcc aggctcaccg
     1561 cgacgccgca cgcgatcgcg agcgaaacga gcgcaagggt cagccgctcg ggagcagcgg
     1621 cgagcgctga gcgcgcgagc gcggcgaggc gacgaccgcg cgaaagggcc ggggtgcccg
     1681 gtggcgttga ggcggtcacg atggtcgagc ctaccgggag cttggggagc gggggcggca
     1741 cacgtccgcc gtccctggtc gcgtcgtcgg cggcggggta tcgtggcggc atggcgacaa
     1801 tagtcttggt agaagatgac ccgaccattg ctgagccctt gacccgcgct ttggccagag
     1861 agggctacac ggtcgaggcg tatgccacag ggatcggtgg ggtcgaggcc agtgaacggg
     1921 ccgacctcat gatccttgac ctgggactac cggacatcga cggcatcgag gtggcgaggc
     1981 gagttcgcga caaggggctc accgtcccca tcctgatgct cacggcacgc gtagacgagg
     2041 tcgatctcgt cgtcgggctc gacgcgggcg cggacgacta cgtgacgaag cccttccgcc
     2101 tcgcggaact cctcgcccgc gtgcgcgccc tcctccgcag gcgagtctcg gccgatggcg
     2161 ccgccgagct cgaggtcaat ggcgtccgca ttgatgtcgc gggccatcgc gccttcgccg
     2221 atggtcgcga actccagctc agcggcaagg agttcgacct gttgcacgcg ctgatcgcca
     2281 atgcggggac ggtcgtgagc cgcgacacgc tcatgcgcga cgtgtggggc gctgatgcgg
     2341 gggcgccctc gaagaccctc gacatgcacg tctcgtggct caggcgcaag ctcggcgacg
     2401 acgcgaacga tccccgcttc atcacgacgg tgaggggact gggtttccgg ttcgagaccg
     2461 gcgtcgagta ggtcctgacc gtgcggcgcc gcctgctgta tgccaccatc tccgcggtcc
     2521 ttgcggcggt cgtgctcctc ggggtacctc tcgcggtcgc ggcagctcag tcggtgagag
     2581 acggctccag aaccgaactc gacgcgcgcg cggtgacggt cgcgcgggcc ctcgaggctc
     2641 gctatatcaa cggtgagacg atcagcgacg ccatactcga gtcgtacgtc ggcggaaaga
     2701 acagcccggc ggcctacgtc ctcgtga
//
LOCUS       DASVKP010000236         6618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_405664, whole genome shotgun sequence.
ACCESSION   DASVKP010000236 DASVKP010000000
VERSION     DASVKP010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405664"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VF362_04710"
     CDS             <1..207
                     /locus_tag="VF362_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX7590263.1"
                     /translation="VSEAALTVHRVLGLRNLSRTDLILDTEGPQFLDVNVAPGMTETS
                     LFPQAIEAAGRTLADVYREIVALA"
     gene            complement(264..1220)
                     /locus_tag="VF362_04715"
     CDS             complement(264..1220)
                     /locus_tag="VF362_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX7590264.1"
                     /translation="MSAPRRGLGRGLGALIPTEGDDAERPSDAFFTPSRPGHGAGATS
                     LGLLPVPGATFAYLPPGDIVPNPRQPRIVFDTDALAELVGSIREVGVLQPIVVRPNPG
                     GPGFELIMGERRLRAAREAGLDAIPAIVRTTEDGDMLRDALVENLHRSQLNPLEEAAA
                     YQQLLDDFGITHEQLADRLSRSRPQISNTLRLLKLPPLVQKRVAAGVLSAGHARALLG
                     LAEPQSMETLAQRIVKEGLSVRATEEAVALGVKASKPRRAAVAARAEAGAYDEIAERL
                     GDRLDTRVRIAGSRAKGSVVIEFGSPEDLARIVEIIDPRGRR"
     gene            complement(1217..2158)
                     /locus_tag="VF362_04720"
     CDS             complement(1217..2158)
                     /locus_tag="VF362_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7590265.1"
                     /translation="MNEDGPRISTDRARNDAFSGVFADDASPLARDMIANERTLRELE
                     EADFPLPPRTRVITVSNQKGGVGKTTTSVNVAAALAKRGATVLVIDSDPQGNASTAVG
                     VTHTSGTPSIYEVMLDGKTLAEVMVRCPDLPSLWCVPATIDLAGADIELVNVMRREFR
                     LSDAVRAFLETHGRDFHYVIIDCPPSLGLLTLNAMVVAGEVLIPIQCEYYALEGLTQL
                     IRTIEMVKKHLNPAIHVSTIVLTMYDGRTNLARDVADEVRGHFPDQTLEISVPRSVRV
                     SEAPSFGQTVITHDPHSSGAVAYLGIAREIAVRGVGA"
     gene            complement(2300..3001)
                     /gene="rsmG"
                     /locus_tag="VF362_04725"
     CDS             complement(2300..3001)
                     /gene="rsmG"
                     /locus_tag="VF362_04725"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX7590266.1"
                     /translation="MIDANAREGVGPGAEVSNTGDPLAGDPRLAEYFGERLGAVSAFR
                     DALVAEGETRGLIGPREAARLWERHLLNSAAVVPFLRSGAVIDVGSGAGLPGLVIAAM
                     EPRREVVLIEPMERRATWLEEIALRLGLANVRVIRARAESAAGLVRAPAVTARAVAPL
                     AKLARWCLPLAADGGQLLFLKGRGAADEVASAAGALRSLRLEAEVLEAVSLPGLEATR
                     VIRLVRAPLGKTPID"
     gene            complement(3024..3512)
                     /locus_tag="VF362_04730"
     CDS             complement(3024..3512)
                     /locus_tag="VF362_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009648471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEX7590267.1"
                     /translation="MSDTPSKQRLDHEGDVAADFLEELLDILDMDGDIDITVEAGRAS
                     VAIVSPDGPSADLKRLVGADGEVLEALQDLARLAVQVETGEMSRLMLDVAGFRAGRKE
                     RLAEVGREAVAKVLETGEPLALDPMNAFERKVVHDVVLAGGLVSESEGEDPHRYVVIR
                     PA"
     gene            complement(3563..4504)
                     /gene="yidC"
                     /locus_tag="VF362_04735"
     CDS             complement(3563..4504)
                     /gene="yidC"
                     /locus_tag="VF362_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012880115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX7590268.1"
                     /translation="MLNTLLLYIEVPVSYVLVGWYKALTFLGLNGSSGWTWALSIVGL
                     VITIRVILIPLFMRQIRSQRAMQLLAPDLKKIQAKYKGKKDQASREAMSRETMELYQK
                     HKTNPFSSCLPLLVQSPIFLALFRVLNTHLKPDVDGVFHPFGALTAKMAEQAHSSTIF
                     GAGISQSFKDGTSGTVKVVAAVLIVAMVTTTFLTQRQITMKNMPKSALEGPMAQQQKM
                     LMYMMPFIFVFSGPFFPIGVLIYWTTTNIWSMGQQFYVIKKNPTPGSEAHKAYQEKLA
                     AKAKANSEGLLSNEPEEPQAPVVTQRAQPQRKNRKRK"
     gene            complement(4518..4835)
                     /gene="yidD"
                     /locus_tag="VF362_04740"
     CDS             complement(4518..4835)
                     /gene="yidD"
                     /locus_tag="VF362_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX7590269.1"
                     /translation="MTGTSVVREVLRVPRYAGLGLIRVYQLTVSPLLGPRCKYYPSCS
                     HYGYQAVERHGLVKGSVLTGWRILRCNPWSYGGVDDVPAVGESMFGRHASPTHIIQTP
                     VTN"
     gene            complement(4832..5176)
                     /gene="rnpA"
                     /locus_tag="VF362_04745"
     CDS             complement(4832..5176)
                     /gene="rnpA"
                     /locus_tag="VF362_04745"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX7590270.1"
                     /translation="MLPADSRMRAASDLQSVMRRGHKSGRVTVVVYLARTAEPTRVIG
                     FAVSKAVGGAVVRNRVRRRLRAIIAAELPSLPAGARVVVRALPAAAAAPFSALSSDVR
                     SALATACARAEA"
     gene            complement(5178..5315)
                     /gene="rpmH"
                     /locus_tag="VF362_04750"
     CDS             complement(5178..5315)
                     /gene="rpmH"
                     /locus_tag="VF362_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX7590271.1"
                     /translation="MTKRTFQPNNRRRAKTHGFRLRMHTRAGRSILAARRRRGRTELS
                     A"
     gene            5557..>6618
                     /gene="dnaA"
                     /locus_tag="VF362_04755"
     CDS             5557..>6618
                     /gene="dnaA"
                     /locus_tag="VF362_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX7590272.1"
                     /translation="MAAHEPIDVIWGKTLDALRANTNLTAQNRSFVRLVKPLALVDDN
                     VFLAVAEDFTKNYLETQLRNVITEALVSVLDRPVRFAVTVDASVVPQDMSDADDEPEP
                     HHSSKSKSKSSSSDDPHLNPRYTFDTFVIGASNRFAHAAALAVAEAPGKTYNPLFIYG
                     DSGLGKTHLLHAIGHYTRHLKPQTRVRYVNSEEFTNDFINAIRDDRKLAFKRHYRDVD
                     ILLIDDIQFLQGKEQTLEEFFHTFNALHNAGKHVVITCDVSPRNLGGIEERMRTRFEW
                     GLMTDVQPPDLETRIAILRKKAALDHVQAPDDVLGYIGSNITTNIRELEGALIRVTAF
                     AALNKQVVDLSLAQVVLKDL"
BASE COUNT         1104 a         2207 c         2250 g         1057 t
ORIGIN      
        1 gtctcggagg cggcgctcac cgtccaccgg gttctgggat tgcgcaactt gtcccgcacc
       61 gacctgatcc tcgacacgga gggcccgcag ttcctcgacg tcaacgtggc gccggggatg
      121 acggagacct ctctcttccc ccaggcgatc gaggcggcgg ggcggacgct cgccgacgtc
      181 taccgcgaga tcgtcgcgct cgcctagggt ctcgttgccg cggcgctcaa ggccggagcc
      241 cggccgtcgc cgcgatccgc gcgctagcgc cgtccgcgcg ggtcgatgat ctccacgatc
      301 cgggcgaggt cctctggcga accaaactca atgacgacgg agcccttcgc cctgctcccg
      361 gcgatgcgga cgcgagtgtc cagccggtcg ccgaggcgtt cggcgatctc gtcgtacgcg
      421 cccgcctccg cgcgggccgc gacggccgcg cggcggggct tcgacgcctt gaccccgagc
      481 gccacggcct cctccgtcgc gcgaacggaa agcccctctt tcacgatgcg ctgggcgagg
      541 gtctccatcg actggggctc ggcgaggccg aggagcgcgc gggcatgccc ggcgctgagg
      601 accccggcgg caacccgctt ctggacgagc ggcggcagct tgagcaggcg gagggtgttg
      661 gagatctggg gacgtgagcg cgacaggcgg tccgcgagtt gctcgtgggt gatgccgaag
      721 tcgtcgagga gctgctggta ggccgcggcc tcctcgagcg gattcagctg agaacggtga
      781 aggttctcca cgagcgcgtc gcggagcatg tctccgtcct cggtcgtgcg gacgatcgcg
      841 gggatcgcgt cgagtccggc ttcccgcgcg gcccggaggc gccgctcgcc catgatgagt
      901 tcgaagcccg ggccaccggg attcgggcgg acgacgatcg gttgcaggac gccgacctcc
      961 ctgatcgagc cgacgagttc cgcaagggcg tcggtatcga agacgatccg cggctggcgc
     1021 gggttcggaa cgatgtcgcc cgggggaaga taggcgaacg tcgctccggg cacggggagc
     1081 aggccgaggg acgtcgctcc ggcgccgtgg cctggccgcg acggcgtgaa gaacgcgtcg
     1141 ctgggccgct cggcgtcgtc gccttcggtg ggaatgaggg cccccaggcc cctccccagg
     1201 ccccggcggg gcgcgctcac gcgcccaccc cgcgcacggc gatctcgcgg gcgatgccga
     1261 gataggcgac cgcgccgctc gagtgggggt cgtgggtgat gaccgtctgc ccaaagctcg
     1321 gcgcctcgga aacgcgaacg gaacggggca cggagatctc cagcgtctgg tcggggaagt
     1381 ggccgcggac ctcgtcggcg acgtcccgcg cgaggttggt gcgaccgtcg tacatggtga
     1441 ggacgatcgt cgacacgtgg atcgcggggt tgaggtgctt cttgaccatc tcgatcgtcc
     1501 ggatgagttg cgtgagtccc tcgagcgcgt agtactcgca ctggatgggg atgagcacct
     1561 cccccgcgac gaccatggca ttcagcgtga gtaggcccag actcgggggg cagtcgatga
     1621 tgacgtagtg gaagtccctg ccgtgcgtct cgagaaacgc gcgaaccgcg tcggacaggc
     1681 ggaactcgcg ccgcatcacg ttgacgagtt cgatgtccgc gccggccagg tcgatggtcg
     1741 cggggacaca ccacagcgac ggcaggtcgg ggcagcgcac catcacctcg gcgagcgtct
     1801 tgccgtcgag catgacctcg tagatggagg gcgtgccgct cgtgtgggtg acgccgacgg
     1861 ccgtcgacgc gttgccctgc ggatcggagt cgatcacgag gacggtggct ccccgcttcg
     1921 cgagggcggc ggcgacgttg acgctcgtgg tcgtcttgcc gaccccgccc ttctggttcg
     1981 agaccgtgat gacgcgtgtg cgcgggggga gggggaagtc cgcctcctca agttcgcgaa
     2041 gcgtgcgctc gttcgcgatc atgtctcgcg cgagggggct ggcgtcgtcc gcgaagacgc
     2101 cggaaaacgc gtcgttgcgc gcgcggtccg tgctgatgcg gggaccgtcc tcgttcactc
     2161 gttctccttg tcgcggccat gaggaccgcg gtcggtggcg gcgtaccgcc gatgtttcac
     2221 gtgaaacgtc gcgagcgcga tgctcccctt ggatgcgcgg gcgtcggggt atgactctag
     2281 tcgccgacgc gggatgcgcc tagtcgatcg gcgtcttgcc gagaggggcg cggaccagcc
     2341 gaatgacccg cgtggcctcg aggccgggca ggctgacggc ctccaacacc tcggcctcaa
     2401 gccggagcga cctgagcgcg cctgcggcgg atgcgacctc gtccgccgcc ccccggccct
     2461 tgaggaagag cagttgaccg ccgtcggcgg ccaggggcag gcaccaacgc gcgagcttgg
     2521 cgagcggcgc caccgcccgc gccgtcaccg caggagcgcg cacgaggcca gccgccgact
     2581 cggcgcgcgc gcggatgacg cggacgttcg caaggccgag ccgaagggcg atctcctcga
     2641 gccatgtcgc gcgccgttcc atcggctcga tgaggacgac ctcgcggcga ggctccatcg
     2701 ccgcgatgac caaacccggc aaccccgccc ccgatcccac gtcgatcacg gcgccgctcc
     2761 ggaggaaggg aaccacggcg gcggagttca acaggtgccg ctcccacagc cgcgccgctt
     2821 cgcgcggccc gatgagccca cgggtttccc cctcggcgac gagcgcgtcg cgaaacgccg
     2881 agaccgcgcc caaccgctcg ccgaagtact cggcgagtcg cgggtccccg gcgagcggat
     2941 ccccggtgtt cgaaacctcc gcgcccgggc cgacgccttc ccgcgcgttc gcgtcgatca
     3001 cgatcgatgt ttcacgtgaa acgctacgct ggccgaatca cgacgtagcg gtgggggtcc
     3061 tcgccctccg actcgctcac gagcccgccc gcgagcacaa cgtcgtggac gaccttgcgt
     3121 tcgaacgcat tcatcgggtc aagtgccagg ggctcgccgg tctccagcac cttggcgacc
     3181 gcctctcgcc ccacctcggc gagtcgctcc ttgcgtccgg cgcggaagcc cgccacgtcc
     3241 agcatgagcc ggctcatctc gccggtctcc acctgcacgg cgaggcgcgc gagatcctga
     3301 agtgcctcaa gcacctcccc gtcggcgccg acgaggcgct tgaggtccgc gctggggccg
     3361 tcgggcgaga cgatcgcgac cgaagcccgt cccgcctcga ccgtgatgtc gatgtcgcca
     3421 tccatgtcga gaatgtccag gagttcctcg aggaagtccg ccgcaacgtc accctcgtga
     3481 tcgaggcgct gcttgctggg ggtgtcgctc atgatcgttc cttcgctggg gtgccggccg
     3541 cccgcgtggg tggccggggg gtctacttgc gcttgcggtt ctttcgctgc ggctgcgcac
     3601 gctgcgtgac aacgggggcc tgcggctcct ccggttcgtt cgagagcagc ccctcactat
     3661 tggccttggc cttggccgcg agcttctcct ggtaggcctt gtgcgcctcg gatcccggcg
     3721 tcgggttctt cttgatgacg taaaactgct gccccatgga ccagatgttg gtcgtcgtcc
     3781 agtagatgag cacgccgatc gggaagaacg gcccggagaa cacgaagatg aacggcatca
     3841 tgtacatgag catcttctgt tgctgcgcca tcgggccctc gagcgccgac ttgggcatat
     3901 tcttcatcgt gatctgacgc tgggtgagga acgtcgtcgt caccatcgcg acgatgagga
     3961 ccgcggcaac aaccttgacc gtccccgagg tcccgtcctt gaaggactgc gagatgccgg
     4021 cgccgaagat cgtggatgag tgcgcctgtt cggccatctt cgccgtgagc gccccgaagg
     4081 gatggaacac gccgtcgacg tcgggcttga ggtgggtgtt gagcacccgg aacagcgcga
     4141 ggaagatagg cgactgcacg aggagcggca ggcacgagga aaacgggttc gtcttgtgct
     4201 tctggtacag ctccatcgtc tcgcggctca tcgcctcgcg ggacgcctga tccttcttgc
     4261 ccttgtactt cgcctggatc ttcttaaggt ccggcgcgag caactgcatc gcgcgctgcg
     4321 aacggatctg gcgcatgaag agcggaatga ggattacgcg gatcgtgatg accaggccga
     4381 cgatcgagag ggcccacgtc catccggagg agccattcaa gccgaggaac gtcagcgcct
     4441 tgtaccaacc tacgagcacg taggagacgg gtacttcgat gtacaacaga agggtgttga
     4501 gcacggatcc cccagcacta gttcgttacg ggcgtttgaa tgatgtgagt gggactcgcg
     4561 tgtcggccga acatcgactc gcccacggcg ggcacatcgt cgactccccc gtagctccag
     4621 gggttgcagc gcaggatccg ccatcccgtg agaacggatc ccttgacgag gccgtgacgc
     4681 tccacggcct ggtacccata gtgggaacac gacggatagt acttgcacct cgggccgagc
     4741 aacggggaga ccgtcaactg gtatacgcga atgaggccga ggccagcgta gcggggcacg
     4801 cgaagaacct cgcggacgac cgacgttccg gtcatgcctc ggccctcgca caggccgtag
     4861 caagggcgga acggacgtcg gaggacaagg cggagaacgg cgccgcggcg gcggcgggca
     4921 gcgctcgcac cacgacgcga gcaccggctg gcaacgaggg aagctccgcg gcgatgatcg
     4981 ctcgaaggcg ccgccttacc cgattccgga ccacggcacc tcctacggcc ttcgatacgg
     5041 cgaaacctat gacgcgcgtc ggctcggccg tccgcgccag atagaccacc accgtaaccc
     5101 tcccgctctt gtgcccgcgg cgcataacgg actggaggtc cgaggccgcc cgcatgcggg
     5161 agtcggcggg gagcacccta tgccgagagc tcggtgcgtc ccctgcggcg gcgggcggcg
     5221 aggatggaac ggccagcgcg cgtgtgcatg cgcaggcgga agccgtgcgt cttggcgcgc
     5281 cgacggttgt tcggctggaa cgtgcgcttg gtcacgggaa ctccgaagtg ttaggggtag
     5341 tcaaatcagc ggaccaaggc tacggggtgg gcgggggacc gtcaaatctc gtcgcccacg
     5401 ttggcctccg cgtccgaaca ggataggctc caccatcagc gcgagttgca tacggaacga
     5461 cacgcggaac gcgaggtaac gaatcggtga cggcaactct gcgggtgtga ataaacctgt
     5521 ggagggctgc ggggagaggc gggaactgtg gcgggaatag ccgcgcacga gcccatcgat
     5581 gtgatctggg gcaaaaccct cgacgccttg cgcgccaaca ccaacctgac ggcacagaac
     5641 cgctccttcg ttcggctcgt caagcccctc gcccttgtgg acgacaacgt gttcctcgcc
     5701 gtcgcggagg acttcaccaa gaactacctc gagacgcaac tacgcaacgt catcaccgag
     5761 gcgctcgtct cggttctcga tcgccccgtg cggttcgcgg tgaccgttga cgcctcggtc
     5821 gttccccaag acatgagcga cgccgacgac gaacccgagc cgcatcactc gtcgaagtcg
     5881 aagagcaaga gttccagttc ggacgacccc cacctgaatc cccggtacac cttcgacacg
     5941 ttcgtcatcg gcgcgtccaa tcgcttcgcc cacgccgcgg cgctcgccgt agcggaagcg
     6001 ccgggcaaga cctacaaccc gctcttcatc tacggcgact ctgggctcgg caagacccac
     6061 ctccttcacg cgatcgggca ctacacccgg cacctgaagc cccagacgcg cgtgcgctat
     6121 gtgaactcgg aggagttcac gaacgacttc atcaacgcca ttcgcgacga ccgcaagctg
     6181 gcgttcaagc gccactatcg cgacgtcgac atcctcctga tcgatgacat ccagtttctt
     6241 cagggcaagg aacagacgct cgaggagttc tttcacacct tcaatgcgct tcacaacgcc
     6301 gggaagcacg tcgtcattac gtgcgacgtc tcgccgagga acctcggtgg gatcgaggag
     6361 cggatgcgaa cgcgtttcga gtgggggctg atgaccgacg tccaacctcc ggacctagag
     6421 acccgcatcg ccattcttcg gaagaaggcg gcgctggatc acgttcaggc gcccgacgac
     6481 gtgctcggct acatcggctc caacatcacg accaacatcc gtgagctcga gggcgccctc
     6541 atccgagtta cggccttcgc ggccttgaac aaacaggtcg tcgacctcag cctggcccaa
     6601 gtggtgctca aggacctg
//
LOCUS       DASVKP010000237         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_821883, whole genome shotgun sequence.
ACCESSION   DASVKP010000237 DASVKP010000000
VERSION     DASVKP010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821883"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VF362_04760"
     CDS             <1..460
                     /locus_tag="VF362_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590273.1"
                     /translation="SRIIKYGRDRVLVDGGLVGDSVIYRHACKLAAKADRVVLVSQYC
                     PTGKLGRLIKAVPHAFYFNPPENASGLNALLIRASMMSSRTRTLYRRKLYLHAKVIIF
                     YLPGGRKVAITGSHNFVRGGVSLGTREIALQTRNPRLIEQIERFITERVR"
     gene            485..1414
                     /gene="rarD"
                     /locus_tag="VF362_04765"
     CDS             485..1414
                     /gene="rarD"
                     /locus_tag="VF362_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEX7590274.1"
                     /translation="MTERLDRQGLIYAFVAYGFWGVFPIFMDQLRPAGALEIVAHRAL
                     WSLAFCAILVVALRRWRRISDVLRDTRTALSLALAALLLAVNWGVFIYAVLTERVADS
                     SLGYYINPLITVALGVVVLRERLHKAQAVAMGLGLVAVAVLGVEVGGVPWIAPTLALS
                     FGAYALLKKRVGATVDARTGFAVETLALLPVAVVLLLVVSARGDTTWGHAGLGHDAWL
                     ASTGAWTAGALLVFAAGAARLPLYVMGLIQYLTPTMTFILAVAYFHENMSVGRWLGFG
                     LVWVALTIITVDTWRRARASAAARAGIVVTEPE"
BASE COUNT          241 a          611 c          619 g          297 t
ORIGIN      
        1 cagccgcatc atcaagtacg ggagggatcg cgtgctcgtg gacggcgggc tcgtcgggga
       61 ctccgtcatc taccgacacg cctgcaaact cgcggcgaag gccgaccgcg tcgttctcgt
      121 ctctcagtac tgcccgaccg gcaagctcgg ccgactcatc aaggccgtcc cccacgcgtt
      181 ctacttcaat ccgccggaga acgcctcggg gctcaacgcg ctcctgattc gggccagcat
      241 gatgtcgagc aggacccgca cgctctatcg caggaagttg taccttcacg cgaaggtcat
      301 catcttctac ctccccgggg ggaggaaggt cgcgatcacg ggctcgcaca acttcgtccg
      361 gggcggggtg tccctcggca cgcgcgagat cgcgctccag acccgcaatc cccggctgat
      421 cgagcagatc gagcgcttca ttacggagcg cgtccgctag cggggcgggt gcgaagatga
      481 ccgggtgacc gaacgcctcg accgccaggg tctgatctac gcgttcgtcg cctacgggtt
      541 ctggggcgtc tttcccatct tcatggacca gctccgcccc gccggagcgc tggagatcgt
      601 cgcccatcgg gcgctgtggt ccctcgcctt ctgcgcgatc ctcgtcgtgg ccctgcggcg
      661 ctggcgcagg atctcggacg tgttgcgcga cacgcgcacc gccctcagcc tcgcgctcgc
      721 cgcacttctc cttgccgtca actggggcgt cttcatctac gccgtcctca ccgagcgcgt
      781 cgccgactcc tcgctcgggt actacatcaa tcccctcatc accgtcgccc ttggcgtggt
      841 ggtcttgcgc gaacgtctcc acaaggcgca ggcggtcgcc atggggctcg ggctcgtcgc
      901 cgtcgccgtg ctgggggtcg aggtgggagg cgtcccgtgg atcgcgccga cgctcgcgct
      961 gagtttcggc gcctacgcgc tgctcaagaa gcgggtgggg gccacggtcg acgcgcggac
     1021 gggattcgcg gtggagacgc tcgccctgct tccggtcgcg gtggtcctcc tgttggtggt
     1081 ctcggcgcgt ggggacacga cgtgggggca cgcggggctt gggcacgacg cgtggctcgc
     1141 cagcacgggc gcgtggacgg ctggcgccct gcttgtcttc gccgcgggag ccgcgcggct
     1201 gcccctctac gtcatgggcc tcatccagta cctcacgccc accatgacgt tcatcctcgc
     1261 ggtggcgtac ttccacgaga acatgtccgt cgggaggtgg ctcggattcg gcctggtgtg
     1321 ggtcgcgctc acgatcatca cggtcgacac gtggcggcgc gccagggcca gcgccgccgc
     1381 tcgcgccggc atcgtcgtca ccgagcccga gtagttcgag cccgaacggc cgtcccgccc
     1441 ctcagcggcg ggagggcgac aggacgagtg tccgggacca ggtcaggtcg tcggcgacgg
     1501 gctcgaattc ggcagcgacg ctccggcgga cgacgacggg gcgggcgtga catacgaggg
     1561 cgtgggcgtc ggcccggcgc ttgggccgcc ttgcgacact ccggggctcg gggtcggacc
     1621 tacccatccg ctcagcgagg gcgatggggt cggcccgacg ctgggggaga cggagggcga
     1681 cgcggagggc gacgccccgt cgcgataggg ccagcaggcg acctgccacg tcttcgaggg
     1741 atcgtaggtc gaggtgatct cgaacacc
//
LOCUS       DASVKP010000238         2894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_902433, whole genome shotgun sequence.
ACCESSION   DASVKP010000238 DASVKP010000000
VERSION     DASVKP010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902433"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1971)
                     /locus_tag="VF362_04770"
     CDS             complement(<1..1971)
                     /locus_tag="VF362_04770"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX7590275.1"
                     /translation="MRSSPWFRPALVVTAIVAWLVLSGVGGAFFGKLAGEQSYDAASF
                     LPKSAESTKEAAQRATFTTVNDIPAIVLLDNVTSPAQVVELTQVMTAAYATPIGGGRT
                     VGDVVDGKPIILPSADGKAIIAIVNLPFSQAFAQDGNAFVGPEVVDAIRAAWAATAHP
                     AGGDFYVTGPLGIFADSFALFGGIDGILLVVAGLVVLIILLVVYRSPVLPLYVLATAG
                     AALSAAGLVVYGLSRSGALSFNGQSQGIMSILVVGATTDYSLLLVARYREELRRIESP
                     YAAMKRAWRRSLAPIVASAITVMLCLLTLLVSDLASNQSLGPVSAIGIFFALLAALTL
                     LPGLLLLGSARARWVFWPRFPAFHPEDVDHVDTLAAVEDHAGIWGRVSRTVATHPRRT
                     WMIAAAGLAVAAAFLPTFKAGGVGDDQQFLQKTESLDGLKIYSAHFPAGQTSPIEITA
                     AAGSLDAVVAAVKGVDGIASVTALTPSLLSGTPPTGVEAPVVVNGRVAINAVTQVNSS
                     SQAATDLVKVVRTAVKAADPTALVGGAAATNLDTNKTTNRDLTVIVPLVLLVILIVLT
                     ILLRALVAPLIIVAANVLSFAATLGVSAIVFNHVLHYPGSSTAVPLFGFVFLVALGVD
                     YSIFLMSRAREEAMRRGTREGIRRALAVTGGVI"
     gene            2032..2790
                     /gene="lipB"
                     /locus_tag="VF362_04775"
     CDS             2032..2790
                     /gene="lipB"
                     /locus_tag="VF362_04775"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX7590276.1"
                     /translation="MDLLDLSVGSRLVDYHDAWDLQRAIHAEVAAGKRPDTLILVEHS
                     GVYTAGKRTASRERPTDDTPVVDVDRGGHITWHGPGQLVGYPIVRLGEPIDVITYVRA
                     LEQATIDLCASLGLDAVRIEGRSGVWFPATGAQRARKVCAIGVRVAKGVTMHGFALNC
                     NPDLGEFGRIIPCGLDDADVTSLSAELGREVTISEVRDAMVGFLECSLADVRWGSANE
                     GAATASAATASAATVSAATASAATVSAATAKEST"
     gene            2787..>2894
                     /locus_tag="VF362_04780"
     CDS             2787..>2894
                     /locus_tag="VF362_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012878796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX7590277.1"
                     /translation="MTIAPEGRRMLRVEARNAETPIERKPEWIRTTATMG"
BASE COUNT          500 a          972 c         1044 g          378 t
ORIGIN      
        1 gatgacgccg cccgtaaccg ccaacgcgcg ccgaatcccc tctcgcgtcc cgcgccgcat
       61 ggcctcctcc ctcgccctgg acatgaggaa gatcgagtag tcgaccccga gcgcaacgag
      121 gaacacgaat ccgaataggg gaaccgcggt gctggatccc gggtaatgga gcacatgatt
      181 gaacacgatc gccgagacgc cgagggtcgc cgcaaacgag agaacgttgg cggcgacgat
      241 gatcagcggc gccacgaggg cccggagcag gatcgtcaac acgatcagga tcacgagcag
      301 cacgagcggg acgatgacgg tgaggtcgcg gttggtcgtc ttgttggtat cgaggttcgt
      361 cgccgcagcc ccgccgacga gcgcggtcgg gtcggcggcc ttgaccgcag tgcgaacgac
      421 cttcaccagg tccgttgccg cctggctgct cgagttgacc tgggtgaccg cgttgatcgc
      481 gacgcggcca ttcaccacca cgggggcctc gacccccgtc gggggcgtcc ccgagagcag
      541 cgacggcgtg agcgccgtca ccgaggcgat cccgtcgacg cccttcacgg cagccacgac
      601 ggcgtcgagc gagcccgcgg ccgccgtgat ctcgattggg ctcgtctggc ccgcggggaa
      661 gtgcgcggag tagatcttca ggccgtcgag ggactcggtc ttctgcagga actgctggtc
      721 gtcgcccacc ccgcccgcct tgaacgtcgg gaggaacgcg gcggcgaccg cgagcccagc
      781 cgcggcgatc atccacgtcc ggcgagggtg cgtggccacg gtccgggaca cgcggcccca
      841 gatccccgcg tggtcctcga ccgcggcgag ggtgtcgacg tggtcgacgt cctcggggtg
      901 gaacgcgggg aacctcggcc agaagaccca acgggcgcgg gcgctcccga ggaggagcag
      961 gcccggcagc agggtgagcg cggcgagaag ggcgaagaag atcccgatcg ccgacaccgg
     1021 gccgagtgac tggttcgacg cgaggtcgga caccaggagc gtcagcaggc acagcatgac
     1081 ggtgatggcc gaagcgacga tgggcgcgag cgagcggcgc caggcgcgct tcatcgcggc
     1141 gtagggactc tcgatccgtc ggagctcctc gcggtagcgc gcgacgagca ggagcgagta
     1201 gtcggtcgtc gccccgacga cgaggatgga catgatgccc tggctctgcc cgttgaagga
     1261 cagcgcaccg gagcgagaga ggccatagac gaccagaccc gcggccgaga gcgcggcgcc
     1321 ggcggtggcg agaacgtaca gcgggagcac ggggctccga tagacgacga gcaggatgat
     1381 gagcaccacg agccccgcca ccaccagaag gatgccgtcg atccccccga agagcgcgaa
     1441 gctatcggcg aagatcccga gcgggcccgt gacgtagaag tccccgccgg cgggatgcgc
     1501 cgtagccgcc caggcggcgc ggatggcgtc caccacctcc ggccccacga aggcgttgcc
     1561 gtcctgcgcg aaggcctggc tgaagggcag gttgacgatc gcgatgatgg ccttcccgtc
     1621 cgccgacgga aggatgatcg gcttcccgtc gacgacgtcg ccgaccgtcc ggcccccgcc
     1681 aatgggggtg gcgtacgcgg ccgtcatcac ctgggtgagt tccaccacct gcgctggcga
     1741 ggtcacgttg tcgagcagca cgatcgcggg gatgtcgttc acggtcgtga acgtcgcgcg
     1801 ctgggcggcc tccttcgtgg actccgcgct cttgggcagg aagctcgctg cgtcgtacga
     1861 ctgctccccc gcgagcttgc cgaagaaggc gccgcccacg cccgagagga cgagccaggc
     1921 gacgatggcg gtaacgacga gggcggggcg gaaccagggc gaactgcgca tggtcatatc
     1981 ctgccccaac gcgggagtgc gcgcgttccg ggcgacgtta gccttgtgac agtggacctt
     2041 ctcgatctca gtgtcggctc gcgcctcgtg gactaccacg acgcgtggga ccttcagcgc
     2101 gcgattcacg ccgaggtagc ggcggggaaa cggcccgaca ccctgatcct cgtcgagcac
     2161 tccggggtct acacggcggg caagcgaaca gcatcgaggg agcggccgac ggacgacacg
     2221 cccgtcgtcg acgtcgatcg cgggggccac atcacgtggc acgggccggg ccagctcgtg
     2281 ggctacccga tcgtccggct cggcgagccc atcgacgtga tcacctacgt gagggcgctc
     2341 gaacaggcga ccatcgacct gtgcgcctcg ctcggcctcg acgccgtccg gatcgaaggg
     2401 cgctcgggcg tctggttccc cgcgacgggt gcgcagcgcg cccgcaaggt ctgcgcgatc
     2461 ggcgtgcgcg tcgccaaggg tgtaacgatg cacggcttcg ccctcaactg caaccccgac
     2521 ctcggcgaat tcgggcggat catcccgtgc ggcctcgacg acgccgacgt gacctcgctc
     2581 agcgctgagc tcgggcgcga ggtcaccatt tccgaggttc gggacgcgat ggtggggttc
     2641 ctcgagtgct ccctcgccga cgtacgctgg ggctctgcaa acgaaggcgc ggcaaccgcg
     2701 agtgccgcaa ccgcgagtgc cgcaaccgtg agtgccgcga ccgcgagtgc cgcaaccgtg
     2761 agtgccgcga ccgcgaagga gagcacgtga cgatcgcgcc cgagggccga aggatgctcc
     2821 gcgtcgaggc acgcaacgcc gagacgccga tcgagcgcaa gcccgagtgg atccgcacga
     2881 ccgcgacgat gggg
//
LOCUS       DASVKP010000239         2521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1478055, whole genome shotgun sequence.
ACCESSION   DASVKP010000239 DASVKP010000000
VERSION     DASVKP010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478055"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VF362_04785"
     CDS             <1..736
                     /locus_tag="VF362_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX7590278.1"
                     /translation="ALTTADNIMTFRAAVKEIALEQGVFASFMPKPLDNAPGSGMHTH
                     FSLFEGDRNAFYEAGAPLQLSDVARKFIAGLLRHAPEVTAITNQYVNSYKRLWGGAEA
                     PSYVCWGSNNRSALVRVPVRKAGRGHSSRIEYRALDSATNPYLAFAVLLAAGLKGVEE
                     NYELPDGAEDDVWELSPGERRAMGYEPLPSSLSDAINVMETSELVAETLGEQVFDFVL
                     RNKRAEWNEYRSQVTAFELERFLPVL"
     gene            754..>2521
                     /locus_tag="VF362_04790"
     CDS             754..>2521
                     /locus_tag="VF362_04790"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEX7590279.1"
                     /translation="MTSRNVSEASALRKAGVADPERVAGQIAQIEAATGLRLATAGVN
                     PAGIDPAGIDLVGIDLAAVADPDLAVLQLLRLVEAASESGDLQRLIAVVGAPAAARLA
                     AVLGASSALGDFLIRHPALVSTLANPEPALPLSASAARAALLVAVGADPAAANPVATI
                     LGAPGVDALRLAYRILLLRVASEDVSSPSPVTIVPMVAEALADLAGAALEAGLALARA
                     EEPGYAETRLAIIAMGKCGARELNYVSDVDVIFVAEPGPGADDARSIAVATRLATAAA
                     RACDRASSELALWQVDANLRPEGKDGALVRNVASHRAYYERWASTWEFQALLKARPVA
                     GDREVGEAYIAAVGPLVWEASRRENFVEDAQAMRRRVEEHVPTGEADRQIKLGPGGLR
                     DVEFTIQLLQLVHGRADESLRIAPTLAALAALRKGGYVGRDEADRMDRAYRQLRVWEH
                     RVQMARMRRTHLLPDSEEGLRALARASGFPSSEALLEAWHAIRRDVRSMHLEMFYRPI
                     LPVVARLSAGEASLDTDAARDRLAAIGFADPEAAQRHLAALTEGVSRKAAIQRQLLPA
                     MIGWFAEVPDPDAGLLAFRTLSE"
BASE COUNT          346 a          920 c          883 g          372 t
ORIGIN      
        1 cgctctcacg acggcggaca acatcatgac gttccgcgcc gcggtcaagg agatcgccct
       61 cgagcagggc gtcttcgcgt cgttcatgcc gaagcccctt gacaacgccc ccggctccgg
      121 gatgcacacc cacttcagcc tcttcgaggg cgatcggaac gccttctacg aggcgggcgc
      181 cccgcttcag ctcagcgacg tggcccggaa gttcatcgcc gggctgctgc gccacgcgcc
      241 cgaggtcacg gcgatcacca accagtacgt caactcctac aagcggctgt ggggcggtgc
      301 ggaggccccc tcctacgtgt gttggggctc caataaccgc tcggcgctcg tgcgagtgcc
      361 ggtgcgcaag gcgggcaggg gtcactccag caggatcgag taccgcgcgc tggactcggc
      421 caccaacccc tatctcgcct tcgccgtcct tctggcggcg ggcctcaagg gcgtcgagga
      481 gaactacgag cttcccgacg gcgcggagga cgacgtgtgg gagctcagcc cgggggagcg
      541 ccgggccatg ggctatgagc cccttccctc ctcgctgagt gacgccatca acgtgatgga
      601 gacgagcgag ctggtcgccg agaccctcgg cgaacaggtc ttcgacttcg tgctgcgcaa
      661 caagcgcgcc gagtggaacg agtaccggtc gcaagtgacc gcgttcgagc tcgagcgctt
      721 cctcccggtg ctctagcgcc cgggaggcgc cccgtgacta gccgcaacgt ctccgaagcg
      781 tcggcgctac gcaaggcggg ggtggccgac cccgagcggg tcgcggggca gatcgcgcag
      841 atcgaggcgg cgaccgggct ccggctcgcc acggcggggg tcaaccccgc gggaatcgac
      901 cccgcgggaa tcgacctcgt ggggatcgat ctggcggcgg tggccgatcc cgacctcgcg
      961 gtcctgcaac tgttgaggct ggtggaggcg gcgtccgagt ccggggacct ccagcgcctg
     1021 atcgccgtgg tcggggcgcc cgcggcggca cgcctcgccg ccgtccttgg cgcatcctcg
     1081 gcgctcgggg acttcctgat ccggcatccg gccctcgtct cgaccctcgc gaatcccgag
     1141 ccagcgcttc ccctctcggc atccgccgcg cgcgcggcgc tcctcgtcgc ggtcggggcc
     1201 gatcccgccg cggccaatcc cgttgcgacg atcctcggcg cccccggcgt cgacgccctg
     1261 cgcctcgcgt accggatcct cctgctacgc gtcgcctcgg aagatgtctc ctcgccttca
     1321 ccggtcacga tcgtccccat ggttgccgag gcgctcgccg acctcgccgg cgcggcgctc
     1381 gaggcggggc tcgcgctcgc cagggcggag gaaccgggct acgccgagac tcgtctcgcg
     1441 atcatcgcga tgggcaagtg cggggcgcgc gaactgaact acgtctccga cgtcgacgtc
     1501 atcttcgtcg cggagccggg accgggtgcg gacgacgcgc gctcgatcgc cgtcgccacc
     1561 cgcctggcga ccgccgccgc ccgcgcgtgc gaccgcgcct cgagcgaact ggccctgtgg
     1621 caggtggacg ccaacctccg gcccgagggc aaggacggcg cactggtccg caacgtcgcc
     1681 tcccaccgcg cgtactacga gcggtgggcg agcacgtggg agttccaggc cctcctgaag
     1741 gcgcggccgg tggcggggga tcgggaggtc ggagaggcgt atatcgccgc cgtcgggccc
     1801 ctggtgtggg aggcgtcgag gcgcgagaac ttcgtggagg acgcccaggc gatgcgcagg
     1861 cgcgtcgagg agcacgtccc gacgggggag gcggatcggc agatcaagct gggtccgggc
     1921 ggccttcgcg acgtcgagtt caccatccag ttgctgcaat tggtccacgg gcgcgccgac
     1981 gagtccctgc ggatcgcgcc gacgctcgcc gccctcgccg cgctgcgcaa ggggggttac
     2041 gtcggtcggg acgaggccga caggatggac cgcgcttacc gtcagctcag ggtctgggag
     2101 cacagggtcc agatggcccg gatgcgcagg acccacctgc tcccggactc cgaggagggg
     2161 cttcgggccc tcgcgcgagc ctcgggcttc ccctcgtcgg aagcgctcct cgaggcgtgg
     2221 cacgcgattc gtcgcgacgt tcgatccatg cacctggaga tgttctaccg tcccatcctc
     2281 cccgtcgtgg cgcggctgag cgccggcgag gcttcactcg acaccgacgc cgcccgcgac
     2341 cggctcgccg cgatcggatt tgccgacccg gaggcggccc agcgccacct cgcggccctc
     2401 acagagggcg tctcccgcaa ggccgcgatc cagcgccagc tccttcccgc gatgatcggg
     2461 tggttcgcgg aggtgcccga tcccgacgct ggcctcctcg cgtttcggac cctctccgag
     2521 c
//
LOCUS       DASVKP010000240         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1635054, whole genome shotgun sequence.
ACCESSION   DASVKP010000240 DASVKP010000000
VERSION     DASVKP010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635054"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VF362_04795"
     CDS             <1..665
                     /locus_tag="VF362_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7590280.1"
                     /translation="LSSFEVAPGRSPSVPWDRRELALVLEGLADLARHAVAPGTFAAF
                     ADEHAGVLTQWQAFADLETAQREALAARAGALGTWVLSNVDMLAEWASRAPQATAGDA
                     LVHGDIRADNILIDGNRAWIVDWPYASVGAPWLDLATFLPSVEMQGGGSAHAIFAAHP
                     LARDVPHEDLRALVAALAGYFFVQSLQPEVPEIPTLRAFQWAQAVPAIRWLRAVEPRV
                     AT"
     gene            complement(672..>2376)
                     /locus_tag="VF362_04800"
     CDS             complement(672..>2376)
                     /locus_tag="VF362_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7590281.1"
                     /translation="GGAVMRGSPARPGSLMLRSVYLKALRDRWLGVVIGTAALGATVW
                     MGVASYASVGNVALDAFRALPKGFLDLMGLNLDLGILGMMFGEMTSAIGPFVLGGLLI
                     AMGADAGAGEERWGTMGLLLANPLSRRRVMVEKSLALITITALGSLLVWGGYWASVGI
                     VGVDATGYHLGSTVIHLAALAFFLGALSMAIGAWTGDTTRAAGIGTAVMVVSFFGAGL
                     LPLLSWGKPWAKIFPWYYLNGADPFLNGVVWGHVAVLAGLGIGCLALAVVGIERRDLK
                     VGETRTTIFDRLARNPRVARYTQKISGSAQVASIAAKSTSDARAALTIAGGGLFYMAA
                     IIGPLFKGVGDPFGKLTDAFPPQLMAMVGGANYATPSGFYTGEIFSIVAPVAVAVVAI
                     SMAAKALAGEERDRTMGVLLANPVTRSRVVLEKAGAMVAMTAVVGLSAFIGVVTGDLI
                     ARLGMSYVDMAAVCVHLTAFGVLMGAIALFAGALTGVSRVATAVGTGVTTLAYAAASF
                     LPLSVRFHGWAKASPFYYYSGSSPLQNGFAWGHIAVLAGASAVFIALSVWAFNRRDLK
                     G"
BASE COUNT          378 a          855 c          829 g          314 t
ORIGIN      
        1 tgctctcctc cttcgaggtc gcgcctggcc gttctccctc ggtcccatgg gaccggcgcg
       61 agttggcgct cgtgcttgag gggcttgccg atctcgcgag gcacgccgtc gcgccgggca
      121 ccttcgccgc gttcgcggac gagcacgcgg gcgtcctcac gcagtggcag gcgttcgcgg
      181 acctcgagac agcccagcgc gaggcgctcg ccgcgcgggc aggagccctc ggcacgtggg
      241 tgctcagcaa cgtggacatg ctcgcggagt gggcgtcgcg cgctccgcag gcgacggccg
      301 gcgacgcgtt ggtccacggc gacatccgcg ccgacaacat cctgatcgac gggaaccgcg
      361 cctggatcgt cgactggccg tacgcgagcg tcggcgcccc ttggttggac ctcgcgacct
      421 ttctcccgag cgtcgagatg cagggcggcg gctccgccca cgcgatcttc gccgcgcacc
      481 ccctcgcccg tgacgtcccc cacgaggacc tgcgggcgct cgttgcggcg ctcgccgggt
      541 acttcttcgt ccagtcgctt caaccggagg tccccgagat cccgacgctg cgtgccttcc
      601 aatgggctca ggccgtaccc gcgattcgct ggctacgggc agttgagccg agagtcgcga
      661 cctgacggtc ctcagccctt gaggtcccgg cggttgaagg cccacacgct gagcgcgatg
      721 aacacggcgc tcgcgcccgc cagaacggcg atgtgccccc aggcgaatcc gttctggagg
      781 ggcgacgagc cggagtagta gtagaacggc gacgccttgg cccacccgtg gaagcggacg
      841 ctcagcggga ggaagctcgc ggcggcgtag gcgagcgtcg tgaccccggt gccgacggcc
      901 gtcgccaccc gggagacccc cgtgagcgcg ccggcgaaca gggcgatggc gcccatgagg
      961 accccgaagg ccgtgaggtg gacgcacacg gccgccatgt cgacgtagct catcccgagc
     1021 cgcgcgatca ggtcgcccgt gaccaccccg atgaacgccg agagccccac gaccgccgtc
     1081 atggcgacca tcgccccggc cttctccagc acgacgcgcg agcgcgtgac ggggttcgca
     1141 agcagcacgc ccatggtgcg gtcgcgctcc tcccccgcca gcgccttcgc cgccatgctg
     1201 atggcgacga cggccaccgc gacaggtgcg actatggaga agatctctcc ggtgtagaac
     1261 cccgacgggg tggcatagtt cgcgccgccg accatcgcca tgagttgggg agggaaggcg
     1321 tccgtgagtt taccgaacgg gtcgccgacg cccttgaaca ggggtccgat gatcgcggcc
     1381 atgtagaaca gcccgccacc ggcgatcgtc agcgcggcgc gggcgtcgct cgtggacttt
     1441 gcggcgatcg aggccacctg ggcggatccc gaaatcttct gggtgtacct cgcgacgcga
     1501 gggttgcggg ccaggcggtc gaagatcgtc gtccgcgtct cgcccacctt gaggtcgcgc
     1561 ctctcgatcc cgaccaccgc gagcgcgagg cacccgatcc cgagccccgc gagcacggcg
     1621 acgtggcccc acacgacgcc gttgaggaac gggtcggcgc cgttgaggta gtaccacgga
     1681 aagatcttcg cccacggctt tccccacgac aggagcggaa gcaggccagc gccgaagaac
     1741 gagacgacca tgaccgccgt gccgatgccg gcggcgcgcg tcgtgtcgcc cgtccacgcc
     1801 ccgatcgcca tcgagagcgc gccgaggaag aacgcgagcg cggcaaggtg gatcaccgtg
     1861 gagcccaggt gatacccggt ggcgtccacg ccgacgatgc cgacagacgc ccagtacccg
     1921 ccccacacca ggagcgagcc gagcgcggtg atcgtgatca gcgcgagcga cttctcgacc
     1981 atcacgcggc gtcggctcaa cgggttggcg agcagcaggc ccatggtgcc ccaccgctcc
     2041 tcgcccgccc cggcgtcggc gcccatcgcg atgagcaggc cgccgagcac gaagggaccg
     2101 atcgccgacg tcatctcgcc gaacatcatc cccaggatgc cgaggtcgag gttgagcccc
     2161 atgagatcga ggaagccctt gggcagggcg cggaacgcgt cgagggcgac gttgccgacg
     2221 ctcgcgtacg aggcgacgcc catccagacg gtggcgccga gcgcggccgt gccgatgacg
     2281 acgccgagcc agcggtcgcg cagcgccttg aggtagacgg agcgcagcat gagcgagccg
     2341 ggcctcgcgg gggagccgcg catcaccgcg ccgcct
//
LOCUS       DASVKP010000241         4967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_1557577, whole genome shotgun sequence.
ACCESSION   DASVKP010000241 DASVKP010000000
VERSION     DASVKP010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557577"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VF362_04805"
     CDS             complement(<1..589)
                     /locus_tag="VF362_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7590282.1"
                     /translation="MRIGIVCPYALDRPGGVQLHVLDLASALTDLGHEVRILAPAAPN
                     FSLPANVDNVGNTIPVPYNGSTARLRFGLVTAWRVRRWLRDGNYDVVHVHEPGAPSLS
                     LLAMWARHGPMVATFHTSNDSSAIMRMAKPFVKPGMEVLDARIAVSPSADRTVKDHFR
                     LDATHIIPNGVGTAAYIEAHPRPAWMGTSDEPTVGI"
     gene            complement(586..1473)
                     /locus_tag="VF362_04810"
     CDS             complement(586..1473)
                     /locus_tag="VF362_04810"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HEX7590283.1"
                     /translation="MNGFLIAMKFSRFVPVWFIRLVAGFGSWIAWLRSAKGVRRLEDN
                     LHRVTGLEGRELRKLSRRGMASAGRYYAEVLELPRMTGAQIDARMRVVGIEESLAAIA
                     ANGRICVALGHSGNWDLVGAWTCRNLGTVRTVAEMLKPREVFDEFVRLREGVGMTVLG
                     LEGGGTFRQLMSIAMRDGGVVALLSDRDLSGTGIAVDMWGRKVRVAPGPAAFALATKT
                     ALVVLYARYERIYGVRRWQAGSRWGSVMEFGGIIEVPEGSKAEQIAQMSQEWATILAD
                     RIATYPEDWHMLQRFGWVE"
     gene            complement(1470..2114)
                     /locus_tag="VF362_04815"
     CDS             complement(1470..2114)
                     /locus_tag="VF362_04815"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX7590284.1"
                     /translation="MLGWLRPHLAKVWKAPAKGLLRLGVTPNAVTITGSIGAAAAALW
                     FFPKGGFNLFTGTMVIMVFAFTDMLDGTMARLSGKASRFGAFLDSTLDRVVDAAIFGS
                     LAWGLIDVDRNTALAAFFCLAFGSFVPYARARAEGLGIEAKVGIAERADRLVFGGIAA
                     VLVGLGLPLAILTYTLWALALAAAITVVQRGAVVARASRKDPTLPAPAAQRASQ"
     gene            complement(2114..2698)
                     /locus_tag="VF362_04820"
     CDS             complement(2114..2698)
                     /locus_tag="VF362_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX7590285.1"
                     /translation="MANEPGEGGGIEGGATANAEMMGGVGDGFERLWTPHRMMYIKGA
                     EDRSGAKPVEGCQLCAKEPVEDRENLIVARGEHCYAILNLFPYNPGHMMICPYRHVAW
                     YTELTDAERDEMGAMTQRAIVVLQRVSGAKGFNTGMNQGATGGAGIAEHVHQHVVPRW
                     VGDSNFLPIIAKTKAMPELLDDTWRRVSEAWKVA"
     gene            complement(2701..4704)
                     /gene="thrS"
                     /locus_tag="VF362_04825"
     CDS             complement(2701..4704)
                     /gene="thrS"
                     /locus_tag="VF362_04825"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017823709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX7590286.1"
                     /translation="MATINVTVDGDERKLDAGTTGADVYADRRDVVVMRLAGELRDLA
                     RELAEGDAIEGVTIGSPDGLAVLRHSCAHVLAQAVQQVNPAAKLGIGPPIENGFFYDF
                     DVAEPFTPESLKELEKVMQRIVNETQSFRRRVVTDAEAIAELSHEPYKVELVGLKSHA
                     GDTGAEGASVEVGAGELTIYENVRKDGTVAWKDLCRGPHVPTTKILTNGWSLTKTSSA
                     YWRGSEKNAPLQRVYGTAWPTKDEMVAFKERQAEAERRDHRKLGVELDLFSFPDEIGS
                     GLAVFHPRGGIIRHEMEEYSRKRHIEEGYEFVYSPHITKASLFQVSGHLDWYADGMYP
                     SMHMDEEVDGDGNITKQGQDYYLKPMNCPFHNLIFRSRGRSYRELPLRMFEFGTVYRN
                     EKSGVVHGLTRARGFTQDDAHIYCTRDQMAGELTTTLNFVLDLLKDYGLTDFFLELST
                     KNPEKFVGDDDAWEVATATLKAVGEASGLEIRDDPGGAAFYGPKLSVQTRDAIGRSWQ
                     MSTIQLDFNLPERFELEYTAPDGTRQRPVMIHRALFGSIERFFAILVEHYAGAFPAWL
                     APVQVLAIPVAEPFNDYLADIVARLRESGIRADIDDSDERFPKKIRTASKEKVPFVLI
                     AGGEDQEAGAVSFRFRDGTQDNQVPVEAAIERIVAAIASREQV"
BASE COUNT          738 a         1655 c         1741 g          833 t
ORIGIN      
        1 ggatccccac cgtcggctcg tccgatgtgc ccatccacgc gggccggggg tgggcctcga
       61 tgtaggcggc cgtgccgacg ccgttgggga tgatgtgggt cgcgtccagg cggaagtggt
      121 ccttgacggt ccggtcggcc gacggcgaga cggcgatgcg ggcgtcgagg acctccattc
      181 ccggcttgac gaagggtttc gccattcgca tgatcgccga cgagtcgttg gacgtgtgaa
      241 acgtcgcgac catcgggcca tggcgcgccc acatggcaag gagcgacagc gagggcgccc
      301 cgggctcgtg cacgtggacc acgtcgtagt tgccgtcgcg cagccagcgg cgaacgcgcc
      361 acgccgtcac caacccgaac cgcaaccgcg cggtcgagcc gttgtagggg acggggatgg
      421 tattgccgac gttgtccacg ttcgccggga gggagaagtt cggcgcggca ggcgcgagga
      481 tgcgcacctc gtgtccgagg tcggtcagcg cgctcgcgag gtcgaggacg tggagttgca
      541 cgccgcccgg gcggtcgagg gcgtatgggc aaacgattcc gatcctcatt cgacccaccc
      601 gaaccgctgc agcatgtgcc agtcctccgg ataggtggcg atgcgatccg cgaggatcgt
      661 cgcccactcc tgggacatct gcgcgatctg ttcggccttg gagccctcgg gcacctcgat
      721 gatgccgccg aactccatga cggaacccca gcgcgacccc gcctgccagc gtcggacgcc
      781 gtagatgcgt tcgtagcggg cgtacaggac cacgagagcc gtcttggttg cgagggcgaa
      841 ggcggcgggg ccgggggcga cgcgcacctt ccggccccac atgtccacgg cgatgcccgt
      901 ccccgacagg tcgcggtcgg acaggagcgc gacgactccc ccgtcgcgca tggcgatcga
      961 catgagttgg cggaaggttc ccccgccctc gagccccaga accgtcatgc cgacgccctc
     1021 gcgcaggcgc acgaattcgt cgaatacctc gcggggtttg agcatctctg cgacggttcg
     1081 aaccgtcccg aggttgcgac acgtccacgc gcccacgaga tcccagttgc ccgagtgccc
     1141 gagcgcgacg cagatgcgcc cgttggcggc gatcgcggcg agggactcct cgatgcccac
     1201 cacgcgcatg cgtgcgtcga tttgggcccc cgtcatccgc gggagctcaa gcacctcggc
     1261 gtagtaccga ccggccgagg ccatcccgcg ccgggagagc ttgcgaagtt cgcgaccctc
     1321 gaggcctgtg acgcggtgga ggttgtcctc gagccgccgc acgcccttgg cgctccggag
     1381 ccacgcgatc cacgagccga agccagcgac cagtcggatg aaccagacgg gcacgaagcg
     1441 cgagaacttc atcgcgatca ggaagccgtt cattgggatg cccgctgagc cgcgggggcg
     1501 ggcagcgtcg ggtccttcct gctcgcgcgg gcgacgacgg cgccacgctg gaccacggtg
     1561 atggcggcgg cgagcgcgag ggcccacagc gtgtaggtga ggatcgcgag cggcagtccg
     1621 aggcccacga gcaccgccgc gattccgccg aagacgagcc gatcggcccg ctccgcgatc
     1681 ccgaccttcg cctcgatccc caggccctcg gcgcgcgccc gcgcgtatgg gacgaacgag
     1741 ccgaatgcga ggcagaagaa ggcagcgagc gccgtgttcc ggtcaacgtc gatgaggccc
     1801 caggcgagcg agccgaagat cgcggcgtcc acgacccggt caagcgtcga gtcgaggaac
     1861 gcgccgaagc gggatgcctt cccgctcagg cgcgccatgg tgccgtcgag catgtcggtg
     1921 aacgcgaaga ccatgatgac catggttccc gtgaagaggt tgaagccccc cttggggaag
     1981 aaccacaggg cggcggcggc ggcgccgatg gatcccgtga tcgtcaccgc gttgggagtc
     2041 actccgaggc ggagcaggcc cttcgcgggc gccttccaga ccttggcgag gtgcggtcgc
     2101 agccagccga gcatcaggcc accttccagg cttcgctcac gcggcgccag gtgtcatcga
     2161 gcagttcggg catcgccttg gtcttggcga tgatgggcag gaagttcgag tcgcccaccc
     2221 atcgtggcac cacgtgctgg tggacgtgct cggcgatgcc cgcgccgccg gtcgccccct
     2281 ggttcatccc cgtgttgaac cccttggcgc cgctcacccg ttgcaggacg acgatggcgc
     2341 gctgggtcat cgcgcccatc tcgtcgcgct cggcgtcggt gagctcggtg taccaggcga
     2401 cgtggcggta ggggcagatc atcatgtggc cggggttgta cgggaacagg ttgaggatcg
     2461 cgtagcagtg ctcgccgcgc gcgacgatga ggttctcgcg gtcctcgacg ggctccttgg
     2521 cgcacagttg gcaaccctcg acgggcttgg cgccggagcg gtcctccgcg cccttgatgt
     2581 acatcatgcg gtgcggggtc cagaggcgct cgaatccatc cccgacgccg cccatcatct
     2641 ccgcgtttgc agtggcgccg ccttcgatgc cgccgccctc gccgggttcg ttcgccacgg
     2701 ctagacctgc tcccgcgacg cgatggccgc gacgatacgc tcgatcgccg cttcgaccgg
     2761 cacctggttg tcctgggtgc cgtcgcggaa gcggaacgag acggctcccg cctcctggtc
     2821 ctcgccgccc gcaatgagga cgaacggcac cttctccttg gaggccgtgc ggatcttctt
     2881 ggggaagcgc tcgtccgagt cgtcgatgtc cgcgcggatc ccggactcgc gaagccgcgc
     2941 cacgatgtcg gcgaggtagt cgttgaaggg ctctgcgacg gggatcgcga gcacctggac
     3001 gggcgcgagc cacgcgggga aggccccggc gtagtgctcg acgaggattg cgaagaagcg
     3061 ctcgatggag ccgaagaggg cccggtgaat catcacgggg cgctgacgcg ttccgtccgg
     3121 cgccgtgtac tcgagttcga accgctccgg caggttgaag tccagttgaa tggtggacat
     3181 ctgccacgag cgcccgatgg catcccgggt ctggaccgag agtttggggc cgtagaacgc
     3241 ggcgccgccc gggtcgtccc tgatctcgag cccggaggct tcgccgacgg ccttgagcgt
     3301 ggccgttgcg acctcccacg cgtcgtcgtc cccgacgaac ttctcggggt tcttggtgga
     3361 cagctcgagg aagaagtccg tgagcccgta gtccttcagg aggtcgagga cgaagttgag
     3421 ggtcgtggtg agttcgcctg ccatctggtc gcgcgtgcag tagatgtggg cgtcgtcctg
     3481 cgtgaagccg cgggcgcggg tcaggccgtg gacgacgccg gacttctcgt tgcggtagac
     3541 cgtgccaaac tcgaacatcc tcagcggcag ttcgcggtac gagcgcccgc gcgaccggaa
     3601 gatcaggttg tggaaggggc agttcatggg cttgaggtag tagtcctggc cctgcttcgt
     3661 gatgttgccg tcgccgtcca cctcctcgtc catgtgcatg gacgggtaca tgccgtccgc
     3721 gtaccagtcg aggtgcccgg agacctggaa gaggctcgcc ttggtgatgt ggggcgagta
     3781 gacgaactcg tacccctcct cgatgtgccg cttgcgggag tactcctcca tctcgtggcg
     3841 gatgatgccg ccgcgagggt ggaacaccgc gaggccggac ccgatctcgt cgggaaagct
     3901 gaagaggtcg agctcgacgc cgagtttgcg gtggtcgcgg cgctcggcct ccgcctgacg
     3961 ctccttgaag gcgaccatct cgtccttggt gggccacgcc gttccgtaga cgcgctgaag
     4021 aggggcgttc ttctcgctcc cccgccaata cgcggacgac gtcttggtca gcgaccatcc
     4081 gttcgtcagg atcttggtgg tggggacgtg cgggccgcgg cacaggtcct tccacgcgac
     4141 ggtgccgtcc ttgcggacgt tctcgtagat ggtcaattcg cccgcgccga cctctacgga
     4201 ggcgccctct gcccccgtgt cgccagcgtg ggacttcagc ccgacgagct ccaccttgta
     4261 cggctcgtgc gagagttcgg cgatggcctc ggcgtcggtg acgacgcggc ggcggaaact
     4321 ctgggtctcg ttgacgatcc gctgcatcac cttctcgagt tccttgaggg actcgggggt
     4381 gaagggctcg gccacgtcga agtcgtagaa gaagccgttc tcgatgggcg ggccgatgcc
     4441 gagtttcgcg gccgggttga cctgctgcac cgcctgggcg agcacgtggg cacacgagtg
     4501 gcgcagcacg gcgagcccgt cggggctacc gatcgtgacg ccctcgatgg cgtcgccctc
     4561 ggccaattcg cgggcaaggt cgcgcaactc gcccgcgagg cgcatgacga cgacgtccct
     4621 gcggtcggcg taaacgtcgg cgcccgtcgt ccccgcgtca agcttccgct cgtcgccgtc
     4681 cacggtcacg ttgatggtcg ccacgtctgc tcctcggtgt ctcgcctgcc cccatgatgc
     4741 taccgagagt cggggtgcgt ggttggcgcg gcagaggaat cgctgcgcta gttagcctgc
     4801 ggtgtcgagc gcggcggcct cgaccgcctt ggcggcctcc accgcgctct tcgcccgctc
     4861 cttcacggcc ttctctgcgt tcctcacctc gcggttcgcc ttgcgcgacg cgcgcttggc
     4921 tttccgcgct gcgcgcctcg ccttgcaggc aacgcgcctt gacctac
//
LOCUS       DASVKP010000242         6372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_903900, whole genome shotgun sequence.
ACCESSION   DASVKP010000242 DASVKP010000000
VERSION     DASVKP010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903900"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VF362_04830"
     CDS             <1..199
                     /locus_tag="VF362_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HEX7590287.1"
                     /translation="PIAGDDDGAKAFAARFLNDLGYDAVDIGPLAESWRVERDTPAYG
                     VRSTVDELRTLAAKAERVQQV"
     gene            325..531
                     /locus_tag="VF362_04835"
     CDS             325..531
                     /locus_tag="VF362_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX7590288.1"
                     /translation="MAMTLRTDSAVEAALARLAERQGVSKHEAAMRAILRDDAEHQLE
                     DDTMAAYDRVSGEYREALDRLGSV"
     gene            537..929
                     /locus_tag="VF362_04840"
     CDS             537..929
                     /locus_tag="VF362_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEX7590289.1"
                     /translation="MPDPTAEPTRPLKFEYVVRIIDREGIGPIRDAGLLDSALARPLT
                     TIMGSDAYPDLATKAAALLHSVCLNHALVDGNKRLAAIATLIFVEINGARCDLTNDEL
                     FDLTMAVASGELRDVDAIAARLRLRPRP"
     gene            complement(907..2118)
                     /locus_tag="VF362_04845"
     CDS             complement(907..2118)
                     /locus_tag="VF362_04845"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX7590290.1"
                     /translation="MSEPISESLRTIHRRGVDAFGATGLAADVGPTVRRVARRRAGRV
                     VGAGLAAVALVATVTVGALGFTGHPVLPGHASVTPHAHSDAERAEIVVYNGDTATDIA
                     EKLAAAAVIGSAQDFVDEANKNPVDAMRIAVGGYWLPTGISASDALAAMLDPSKRQAA
                     NLTIQPGTTEEQIFRQLEGTLGIRNADFYSAAEDPASIGLPAEANGDVEGWLGAYSYH
                     FDEGTTATEVLSSMIAATIFHLDEQGVAPADRQRVLTEASLIDMGAPFGDDRSKIARV
                     ILNRLAAGIPVDLEAGVSYRAGLDGVLTPQANRNIYSPQNTYLVPGLPPAPLWSPSAA
                     SIDAALHPADGPWLYFTTVNPSTGETGYSTTYEEHQRNLALLLAWDQANLSDLRDELG
                     TLVGTTDAGGA"
     gene            complement(2115..2672)
                     /locus_tag="VF362_04850"
     CDS             complement(2115..2672)
                     /locus_tag="VF362_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7590291.1"
                     /translation="MKAWARQLETLVDERGAALFGYAYVLTGDKARAEDLVQDALVRT
                     FQRGRADLGIDAAHAYVKRAISSAFIDSGRRLRARPQISDVGAEAVGPDHADGVALAL
                     SLHASLLTLPPRERACVVLRYLEDMSVASIADELGLATGSVKRYLSDGVARLRALHGS
                     FDLAASDLLTGGGTTVLVLPKGDRS"
     gene            complement(2761..4725)
                     /locus_tag="VF362_04855"
     CDS             complement(2761..4725)
                     /locus_tag="VF362_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7590292.1"
                     /translation="MPKGTRKREGAHQALPTEHAGRDHAPASDAEFRYLASSLPIIVW
                     TTTPHGSMDYLNDEVERYTGRPKEELLGDRWVALMHPDDVDRVVGTARSAMEVGEPYR
                     LHVRLLGADGEYRWFAISAGPQRDSSGAILRWWGNATDIHDFQFLREEASARAAERER
                     ILESIGDGVFATDHDLCVTYMNGSAEAAISMTREELVGRRIWEAFPEVTDTEAEGFYR
                     KALAGEPQHFERYNARLDRWFEVSAYPSEHGVTAYFRNVTGLKSRASQFAQSQRLEAV
                     GQLTGGIAHDFNNLLTVVVGGSEALASEPGLSDEAREMTDLISRAALRGAELTHRLLA
                     FASKQVLRPEAIDLGLAMEHFTALIERTLGADVEITSHVEEGLPLAEVDPGEFENAIL
                     NLALNARNAMPEGGRLALDARGVVLDEASPAAGSEILPGPYVVVSVSDTGHGIPKDTL
                     PKIFEPFFTTKPQGEGSGLGLAMVWGFAKQSGGNVTVYSEVGLGTTFRLYLPVSTQGE
                     PASTFASASALASSRRAARSGTGHVLLAEDDELVRRFAADRLRSLGYVVTEAGSGPEA
                     LESLESIPELGLLFTDVVMPGGLTGRDLADAVVALRPGTPVLFASGYAENVIVHDGKL
                     DQGVDLLTKPYTGSQLAERVARAIAGREPE"
     gene            4818..5375
                     /locus_tag="VF362_04860"
     CDS             4818..5375
                     /locus_tag="VF362_04860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX7590293.1"
                     /translation="MSAPVPEYVVTRKRVKNLRITVKAPEGEVRVTAPAFVSERAIRD
                     FVASRADWIAKHQARIALQPAPLEPGPDANRLRRELRRAIPPLLAVWAPRVGVPTPDF
                     TIRRMTTRWGTCNVRARRITLNLELARREPELLEYVVVHELAHLIESGHNQRFYAVMD
                     RALPDWRLKRKALNHGGIARDFADG"
     gene            complement(5438..>6372)
                     /locus_tag="VF362_04865"
     CDS             complement(5438..>6372)
                     /locus_tag="VF362_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7590294.1"
                     /translation="RVMDAARRFADANGLIIAGVLGKPHTQDEVRAVFERHSQPRKSD
                     SAESELAPTSWRATEFQVAFRAAVMSDELAVAYQPKVDCKTGAVVGYEALARWTHPER
                     GSISPDEFVPVAERFGLISLLTERVMGDALRWFAGGEHAEGVRIAINISAGEFDEPSL
                     DKRFVTACEEAGVAPTDVVLEITETGAMADPARSLQLLTRLRLQGFHLSLDDFGTGYS
                     SMLQLARLPFSELKVDRSFVTTAASSDESVIVVRSIIDLGHALGMQLTAEGVEDASVL
                     ALLTDLGCDYAQGFYIARPMLPDALEAWAAARSI"
BASE COUNT          978 a         2205 c         2205 g          984 t
ORIGIN      
        1 gcccatcgcg ggcgacgacg acggcgccaa ggccttcgcg gccaggttcc tgaacgacct
       61 gggttatgac gccgtcgata tcggcccgct cgccgagagt tggcgcgtcg agcgcgacac
      121 cccggcgtac ggcgtccggt cgacggtcga cgagttgcgc accctcgccg ccaaggccga
      181 acgggtccag caggtctagg ggaggctggc gcgtcgaggg gcgctcgcag cgagggaggg
      241 gtgtcgcgtc acggagcgcc cggcaccgcg ttagccgaca ccgggtggta tcgaagtgat
      301 accacttcgc gctatcctgg caccatggcc atgaccctcc gcaccgactc cgccgtcgag
      361 gccgcgctcg cgcgcctcgc cgagcgccag ggcgtctcca agcacgaggc ggcgatgcgc
      421 gccatcctgc gcgacgatgc cgagcaccag ctcgaggacg acacgatggc ggcctacgac
      481 cgcgtcagcg gcgaataccg cgaggcgctt gaccgcctcg gttccgtgta accgaagtgc
      541 ctgacccaac tgcggagccc acccgcccgc tcaagttcga gtacgtggtg cggatcatcg
      601 atcgcgaggg gattggcccc attcgggacg ctggcctgct cgactccgct ctcgcccgcc
      661 cgctcaccac gattatggga agcgacgctt acccagacct cgcgaccaag gcggcggcgc
      721 tcctgcactc ggtatgcctc aatcacgccc tcgtcgacgg caacaagcgg ctcgccgcaa
      781 tcgcgacgtt gatctttgtc gagatcaacg gcgcgcgctg cgacctgacg aacgacgagc
      841 tgttcgacct cacgatggcg gtggcgtctg gcgagttgcg cgacgtcgac gcgatcgcgg
      901 ctcggctcag gctccgcccg cgtccgtagt gcccacgagc gttccgagtt catctcgcag
      961 gtcgctaagg ttcgcctggt cccatgccag caggagggcg aggttccgct ggtgctcctc
     1021 gtacgtcgtc gagtacccgg tctctccggt ggaggggttg acggtagtga agtacagcca
     1081 cggcccatcg gccgggtgga gcgcggcgtc gatcgacgcc gcactcggtg accagagcgg
     1141 cgccggcggc aggcccggca ccagatacgt gttctggggg ctatagatgt tgcggttggc
     1201 ttgcggggtg agaacgccgt ccaggcccgc tcggtaggag actcccgcct caagatcgac
     1261 cggaatgccc gccgcgaggc ggttcaggat tactcgcgcg attttggagc ggtcgtcgcc
     1321 gaacggggca cccatgtcga tgagcgaggc ctccgtgagc acgcgttgcc gatccgccgg
     1381 tgcgacgccc tgctcgtcga gatggaagat cgtcgccgcg atcatgctcg agagcacctc
     1441 ggtcgccgtc gtcccctcgt cgaagtggta cgagtaagcc ccgagccacc cttccacgtc
     1501 cccgttcgcc tcggcgggga ggccgatcga cgcggggtcc tccgcggcgc tgtagaagtc
     1561 cgcgttgcgt atgccgagtg tcccctcgag ttgcctgaag atctgctctt cggtggtgcc
     1621 cggctgaatg gtgaggtttg cggcctgccg cttcgacgga tcgagcatcg ccgcaagtgc
     1681 atcggacgcg ctgatgcccg tggggagcca gtacccgcct accgcgatcc gcatggcgtc
     1741 gacggggttc ttgttcgcct cgtccacgaa gtcctgggcg gatccgatga cggcggcggc
     1801 ggccagcttc tcggcgatgt ccgtggccgt gtccccgttg tacaccacga tctccgcgcg
     1861 ttcggcgtcc gagtgcgcgt ggggggtcac ggaggcgtgt ccggggagca ccgggtgccc
     1921 cgtgaacccg agcgcaccaa cagtcacggt cgcgacaagc gccacggccg caaggcccgc
     1981 ccccacgacc cgtcccgcgc gtcggcgggc aacgcgtcgg accgtcggcc cgacgtccgc
     2041 ggcgagcccg gtcgcgccga acgcatccac ccctcggcgg tggatcgttc ggaggctctc
     2101 ggaaatgggc tcgctcatga gcggtctccc ttcgggagga cgaggacggt ggtgccgccc
     2161 ccggtgagaa ggtcggaggc ggcgaggtcg aagctcccgt ggagcgcgcg caggcgggcg
     2221 acgccgtcgc tgaggtagcg cttgacggag ccggttgcga ggccgagctc gtccgcgatg
     2281 gacgccacgc tcatgtcctc gaggtagcgg agcaccacac acgcccgctc gcgcgggggg
     2341 agggtcagga gggacgcgtg gagcgagagc gcgagcgcga cgccgtcggc gtggtcgggc
     2401 ccgacggcct cggcccccac atcggagatc tgcggccgcg cgcgcaggcg gcgacccgag
     2461 tcgatgaacg ccgacgaaat ggcccgctta acgtacgcgt gggcggcgtc gatccccagg
     2521 tccgcgcgcc cgcgctggaa ggtgcggacg agcgcgtcct ggacgaggtc ctccgcgcgg
     2581 gccttgtccc cggtcagcac gtaggcgtat ccaaagaggg ccgcgccgcg ctcatcgacg
     2641 agcgtctcaa gttgcctggc ccaggccttc acggatcgat tcctgttctc ataccgggta
     2701 gtacgcaccc gcgccacaaa gggttgggtg gaacgagcga ggcccgcgcc tgagggcgcc
     2761 ctactcgggc tcgcgaccgg cgatcgctcg cgcgacccgc tccgccaact ggctacccgt
     2821 gtagggcttg gtgaggagat ccacgccctg gtcgagcttt ccgtcgtgga cgatgacgtt
     2881 ctcggcgtag cccgaggcaa agagcacggg ggtcccgggc ctcagcgcga ccacggcgtc
     2941 cgcgagatcg cggcccgtga ggccgccagg catgacgaca tccgtgaaca ggagtccgag
     3001 ttccgggatc gactcgaggg actcgagcgc ctcgggcccc gacccggcct cggtcaccac
     3061 gtagccgagc gagcgcagac ggtcggccgc aaaccggcgc acgagttcgt cgtcctcggc
     3121 gagcaagaca tggccggtgc ccgaccgcgc ggcgcggcgg ctgctcgcca gcgcggaggc
     3181 ggaggcgaag gtggaggcgg gctccccttg agtcgacacg gggaggtaga gccggaacgt
     3241 cgtgccgagt ccgacctcgg aatagaccgt cacattcccg cccgactgct tggcgaatcc
     3301 ccagaccatg gcgagtccca gtccggatcc ctcgccttgg ggcttcgtgg tgaagaaagg
     3361 ctcgaagatc ttcggaaggg tgtccttggg gataccgtga cccgtgtcgc tcacggaaac
     3421 gacgacgtag gggccgggaa ggatctcgct acctgccgcc gggcttgcct cgtcgaggac
     3481 gacgccccgc gcgtcgaggg cgagcctgcc tccctcgggc atggcgttgc gcgcgttgag
     3541 cgcgaggttg aggatcgcgt tttcgaactc gccggggtcc acctcagcga gcgggagacc
     3601 ctcctcgacg tgggaggtga tctcgacgtc tgcccccagg gtccgctcga tgagcgccgt
     3661 gaagtgctcc atcgcgagcc cgagatcgat cgcctcgggt cggagaacct gcttgctcgc
     3721 gaaggcgagc aggcggtggg tcaattcggc cccgcgcagg gcggctcggg agatgaggtc
     3781 cgtcatctcc cgagcttcgt cgctcaggcc gggctcggag gcgagcgctt cgcttccccc
     3841 gacgacgacg gtgagcaggt tgttgaagtc gtgagcgatg cctcccgtca actgtccgac
     3901 cgcctcgagg cgttgcgact gcgcgaactg actggcacgg gacttcaacc ccgtgacgtt
     3961 ccggaagtac gcggtcaccc cgtgctccga cgggtaggcg ctcacctcaa accagcgatc
     4021 cagcctcgcg ttgtaccgct cgaagtgctg aggttcgcca gccagcgcct ttcggtagaa
     4081 gccctccgcc tccgtgtccg tgacctcggg gaacgcctcc cagatcctgc gcccaacgag
     4141 ttcctcccgc gtcatgctga tcgcggcctc ggcgcttcca ttcatgtagg tcacgcaaag
     4201 gtcgtggtcg gtggcgaaga cgccgtcccc gatgctctcc aggattctct cccgttcggc
     4261 ggcacgcgcg gaggcctcct cgcggaggaa ctggaagtcg tggatgtcgg tcgcgttgcc
     4321 ccaccaacgt aggatcgcgc ccgaggagtc gcgctgtggg cccgcggaga tcgcgaacca
     4381 ccggtactcg ccgtcggccc ccaagaggcg gacgtgaagg cggtaaggct cccccacctc
     4441 catggcactg cgggcggttc cgacgacgcg gtcgacgtcg tcggggtgca tgagcgcgac
     4501 ccaccggtcg ccgaggagct cctctttcgg ccgcccggtg taccgctcca cctcgtcgtt
     4561 caggtagtcc atcgacccgt ggggagtcgt cgtccagacg atgatcggca gagaactcgc
     4621 gaggtacctg aattccgcgt cgctcgctgg cgcgtggtca cgtccggcgt gttcggtggg
     4681 caatgcctgg tgtgcgcctt cgcgcttccg agtccctttg ggcacgctcc attctaggtt
     4741 ccggtcggca attggcgcag gatggtgggg aaattcgttc ccctgtgcgc cagccggggt
     4801 cgtacgctgt cctcaccatg agcgcgccgg tccccgagta cgtcgtcacg cgcaagcgcg
     4861 tgaagaacct gcgcatcacg gtcaaggccc ccgagggcga ggtccgcgtc acggctcccg
     4921 ccttcgtctc ggagcgcgcg atccgcgact tcgtcgcctc cagggccgac tggatcgcga
     4981 agcaccaggc gcgcatcgcg ctccagcccg cgcccctgga accgggcccc gatgccaacc
     5041 gcctgcgccg cgagctccgc cgcgccatcc cgccgctgct cgccgtgtgg gccccgcgcg
     5101 tcggcgtccc gacccccgac ttcaccatcc ggcgcatgac gacccgctgg ggcacatgca
     5161 acgtccgcgc gcggcgcatc acgctcaacc tcgagctcgc gcgtcgggag ccggagctcc
     5221 tggagtacgt cgtggtgcac gagctcgcgc atctgatcga gtctggccac aaccagcggt
     5281 tctacgccgt gatggaccgc gcgctcccgg actggcggct caagcgcaag gcgctgaacc
     5341 acggcggcat cgcgcgcgac ttcgccgacg ggtaggccag ccccacccgg aggctaagcg
     5401 ccagcgccct agccgacggt ccagcgaggc cagcgcccta gatcgaccgg gccgccgccc
     5461 aggcctcaag cgcgtcgggc aacatgggcc gggcaatgta gaacccctgg gcgtagtcgc
     5521 agccgaggtc ggtaaggagc gcgagaaccg aggcgtcctc caccccctcg gccgtgagtt
     5581 gcatgcccag cgcgtggccg aggtcgatga tggagcgcac cacgatgacg gattcgtcgg
     5641 aggaagcggc cgtcgtcacg aacgaccggt ccaccttgag ttcgctgaac gggagccgcg
     5701 ccaactggag catcgacgag tagcccgtgc cgaagtcatc gagcgacagg tggaacccct
     5761 gcagtcggag gcgggtcagc aactgcaggg agcgcgccgg gtccgccatc gcgcccgtct
     5821 cggtaatctc aagaaccacg tcggtaggag ccaccccggc ctcctcgcac gcggtgacga
     5881 agcgcttgtc gagggacggc tcgtcgaact cgccggccga gatgttgatg gcgatgcgca
     5941 cgccctcggc gtgctcgcct ccggcgaacc agcgcagcgc gtcccccatg acccgttccg
     6001 tgaggagcga gatgaggcca aaccgctcgg cgacggggac gaactcgtcg ggagaaatcg
     6061 agccccgttc ggggtgagtc caccgggcga gcgcctcgta cccgacgacc gctcccgtct
     6121 tgcagtcgac cttgggctgg taggccaccg cgagttcgtc gctcatgacg gccgcgcgga
     6181 aggccacctg gaactcggtc gctcgccaac tcgtcggggc gagctccgac tcggccgaat
     6241 cggacttccg gggctgcgaa tggcgttcaa acaccgcccg aacctcgtcc tgggtgtgtg
     6301 gcttccccag gactcccgcg atgatcaggc cgttcgcatc cgcgaagcgg cgggcggcgt
     6361 ccatcacgcg gc
//
LOCUS       DASVKP010000243         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_161123, whole genome shotgun sequence.
ACCESSION   DASVKP010000243 DASVKP010000000
VERSION     DASVKP010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161123"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..296
                     /locus_tag="VF362_04870"
     CDS             126..296
                     /locus_tag="VF362_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590295.1"
                     /translation="MGEIDKHRDALGEVKFPPEVESALAAVTELPDDPEKQAEFFDKV
                     QEALGNRLRDDS"
     gene            304..1119
                     /locus_tag="VF362_04875"
     CDS             304..1119
                     /locus_tag="VF362_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX7590296.1"
                     /translation="MRLDRALAARGLARSRTHAQALIAAGSVMVGGVVARRSSFEVGE
                     DVEITLAATADAYVSRGAHKLIGALDAFGVRGLTIAGRACLDAGASTGGFTQVLLERG
                     AASVLAIDVGHGQMAPSVAEDPRVTSREGVNVRDLEPPGEGAGVDLIVADLSFISLSL
                     AVEPLAAWLRPGGDMMLMVKPQFEVGAGRLGRGGVVRRPEDMADAVASVARSMASAGF
                     GIADVSRSVMSGSRGTVEFFVWGRRTWQAGGEVQPLGEEALGTVIEREVETSA"
     gene            1116..1976
                     /locus_tag="VF362_04880"
     CDS             1116..1976
                     /locus_tag="VF362_04880"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX7590297.1"
                     /translation="MTRRILVVAHPSRRTMRGALQVALDTLIALGTEPVTEYTHGESD
                     PVESALVLGGDGTMLHAVALTHGGDIPLLGVNLGRVGFLAEAEGDDVKIAAEALARRT
                     YSVEERRTLELTVTFPDGTTKDGWALNEAAVERATPHRTLEVEVEVDGSPLSAFGCDG
                     VVVSTATGSTAHAFSAGGPVIWPNVDALLFIPLAAHALFSRPLVVGPDSTFGVLIAPT
                     SSTPGTIVCDGARAIDAPLGARILFRRSAYTVKLARLDDETFTHRLTTKFSLPTKGWR
                     RDLDTATVED"
     gene            1979..>2541
                     /locus_tag="VF362_04885"
     CDS             1979..>2541
                     /locus_tag="VF362_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7590298.1"
                     /translation="MLHELSIENLGVIESARLTLGAGLTVVTGETGAGKTMVLTGLAL
                     VTGGKAPTAIVRSDAEVAFAEAIIDVPEGSDAAALLEEAGAVRNDDGTITVARTLGAT
                     ARSRTVVGGRQVPQAVLAECAEELVTVHGQADQVRLRSAAKQRETLDRYAGEAHLATL
                     AEYRATWASLAEIRGELERMQRGADVER"
BASE COUNT          364 a          877 c          917 g          383 t
ORIGIN      
        1 gagtattcgg gcgagccggg catggaccgg ctgcgcgtgg cgtgcgtcgc ggcatgggac
       61 gccgcggacc gaagtgagcc gattcacatt gagccggacg acgagaacgg gttatggtga
      121 gtgacatggg agaaatcgac aagcatcgtg acgcgctcgg cgaggtcaag tttcctcccg
      181 aggttgagag cgcgcttgcg gcggtaaccg agctccccga cgacccggag aagcaggcgg
      241 agttcttcga caaggttcag gaagctctcg gcaaccgcct gcgcgacgac tcctagcccc
      301 ccgatgcggc tcgatcgcgc cctcgcggcg cggggtttgg cgcggtcccg cactcacgcg
      361 caggcactga tcgccgcggg gtccgtcatg gtcggcgggg ttgtcgcccg ccgctcgtcg
      421 ttcgaggtgg gcgaggacgt cgagatcacc ctcgccgcca cggcggacgc ctatgtgtcg
      481 cgcggtgccc acaagctgat cggcgcgctc gatgccttcg gcgttcgcgg cctgacgatc
      541 gccggacggg cgtgccttga cgcgggggcg tcgacggggg gcttcaccca ggttctgctc
      601 gagcgcggtg ccgcaagcgt cctcgccatc gacgtcggcc acgggcagat ggcgccctcc
      661 gtcgccgagg atcccagggt aacctcccgc gaaggtgtga acgtccgcga cctcgaaccg
      721 cccggcgagg gcgcgggggt ggacctgatc gtcgccgacc tttccttcat ctccctgtcg
      781 ctcgccgtcg aaccgctcgc cgcctggctt cgtcctgggg gagacatgat gctcatggtc
      841 aagccccagt tcgaggtggg cgccggccgc ctgggtcggg gcggggtcgt ccgacggcct
      901 gaggacatgg ctgacgcggt ggcctcggtt gcgcgctcga tggcgagcgc gggcttcggc
      961 atcgcggacg tctcgcgaag cgtcatgtct ggctcgcgtg gaacggtgga attcttcgtg
     1021 tggggtcgcc gaacgtggca ggctgggggc gaagtccagc ccctgggcga ggaggcactg
     1081 gggaccgtga tcgaacgcga ggtggaaaca agcgcatgac ccgccgcatt ctcgtggttg
     1141 cgcatccgag ccgccgcacc atgcgcggcg cactccaggt cgccctcgac acgctgatcg
     1201 ccctcgggac cgaacccgtc accgagtaca cccacggcga gagcgatccg gtcgagagcg
     1261 cgctcgtcct tgggggtgac gggaccatgc ttcacgccgt cgccctgacg cacgggggcg
     1321 acatcccgct gctcggggtg aacctcggcc gcgtcggctt cctcgctgag gccgaggggg
     1381 acgacgtcaa gatcgcggca gaggcgctcg cgcgccgcac ctactcggtg gaggagcgcc
     1441 gcacgctcga actgacggtg acgttcccgg acggcactac caaggacggt tgggcgctca
     1501 acgaggctgc ggtcgagcgc gccactcccc accgcactct cgaggtcgag gtcgaggtgg
     1561 atgggagccc gctgtccgcg ttcgggtgcg acggggtcgt cgtgtccacg gccacgggct
     1621 cgacggcgca cgcgttttcc gcggggggac cggtcatctg gcccaacgtc gacgcgctgc
     1681 tgttcatccc gctcgctgcg catgccctgt tctcccggcc cctcgtcgtc ggccccgact
     1741 ccaccttcgg cgtgctcatc gcgccgacct cctcgacgcc cgggacgatc gtctgcgacg
     1801 gcgcgcgggc gatcgatgct ccgctcggcg ccaggatcct gttccgccgc tccgcgtaca
     1861 cggtcaagct cgcgcgcctg gacgacgaga ccttcaccca ccgcctcacc accaagttct
     1921 cgctgccgac caaggggtgg cgccgcgacc tcgacaccgc gaccgtggag gactagtcgt
     1981 gctccacgaa ctgtccatcg agaaccttgg cgtgatcgaa tcggcccggc tgacgctcgg
     2041 cgctgggctg accgtcgtca caggggagac cggcgcgggc aagacgatgg tgctcactgg
     2101 cctcgcgctc gtgaccggcg gcaaggcccc gacggccatt gtccgttctg acgcggaggt
     2161 cgcgttcgcc gaggccatca tcgacgttcc cgagggcagc gacgcggccg ccctcctgga
     2221 ggaggcgggc gcggtccgca acgacgacgg caccatcacc gttgcgcgga ccctcggggc
     2281 cacggcgcgc agccgcacgg tcgtgggcgg gaggcaggtt ccccaggccg tcctcgcgga
     2341 atgcgcggag gagctcgtca ccgtccacgg ccaggccgac caggtgcggc taaggtccgc
     2401 ggccaagcag cgggagaccc tggaccgcta cgcgggcgag gctcacctgg cgacgctcgc
     2461 ggagtaccgc gcgacctggg cctcgctcgc cgagatccgc ggcgagttgg agcggatgca
     2521 gcgcggagcc gacgtggaac g
//
LOCUS       DASVKP010000244         3957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_161341, whole genome shotgun sequence.
ACCESSION   DASVKP010000244 DASVKP010000000
VERSION     DASVKP010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161341"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VF362_04890"
     CDS             <1..853
                     /locus_tag="VF362_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX7590299.1"
                     /translation="GDWLDSHPTEARDVIRKSLQASAARMAARKAREATRRKGLLESG
                     GMPGKLRDCQSRNPAESEIYIVEGDSAGGSAVRGRDPQTQAILPLRGKILNVERARLD
                     RALGNNEVQALITAFGTGIGEDFDLAKCRYHKIILMADADVDGQHIRTLLLTLLFRYM
                     RPLIDAGYVYLAQPPLFKIKWSNNDHEYVFSDRERDALLADGAKAGKKIPKDNAIQRY
                     KGLGEMDYSELWETTMNPVSRTLLQVTMDDAAAADEVFSVLMGEDVESRRSFIQRNAK
                     DVRFLDI"
     gene            855..3449
                     /gene="gyrA"
                     /locus_tag="VF362_04895"
     CDS             855..3449
                     /gene="gyrA"
                     /locus_tag="VF362_04895"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX7590300.1"
                     /translation="MTGHDKIEPIDIQVEMQRSYLDYAMSVIVGRALPEVRDGLKPVH
                     RRVIYAMFDGGYRPDRAYSKCSRVVGDVMGKYHPHGDSAIYDTLVRLVQDWSLRYPLV
                     DGQGNFGSPGNDPAAAPRYTECRMAPLAMEMVRDIDKDTVDFQDNYDGRTQEPSILPA
                     RFPNLLVNGSAGIAVGMATNIPPHNLREVASGAQWFLENPDASKEVLLEALIERIKGP
                     DFPTGAMILGRAGIDQAYRTGRGSITMRAVVEVEEIQGRMCLVVSELPYQVNPDNLAQ
                     KIAELVRDGKIAGIADIRDETSGRTGQRLVIVLKRDAVAKVVLNNLYKHTQLQDNFGA
                     NMLALVDGVPRTLTLDAFIRHWVAHQIEVIVRRTQFRLREAEREIHILRGYLKALDAL
                     DAVIALIRAAATVDEARERLMTFLDVDEIQANAILNLQLRRLAALERKKIEDDHASLE
                     SEISDLNNILASEARQRTIIATELGEIVAKYGDERRTIIAPHEGDMDIEDLIAEEEVV
                     VTITRSGYAKRTKSSLYRSQKRGGKGVRGASLRADDVVQHFFVTTTHHWLLFFTNLGR
                     VYRAKAYELPDGARDAKGQHVANMLAFQPGEEIAEVLDLKTYADKPYLVLATKRGLVK
                     KTRLEEYDSNRSGGLIAINLRPIGREEDAVADDAPTGVALTDELMAARLVSDEDDLML
                     VSRKGQSIRFTADGDALRPMGRATSGVTGMRFREDDELLAMDVVRDGMFSFVATEGGY
                     AKRTDVAEYRSQGRGGLGIKVAKLQEERGDLVGALLVDEADEVLVVMENGKVMRADVV
                     GVPAKGRDTMGVIFAKPDEGDRIVAIARNSERSLDGDGADDATGDAAASAAAPGARVS
                     DEGATDEA"
BASE COUNT          731 a         1341 c         1311 g          574 t
ORIGIN      
        1 cggcgattgg ctggactcgc atcccacgga ggcgcgagac gtgatccgga agtcgctaca
       61 ggcatccgcg gcccgcatgg cggcgcgcaa ggctcgcgag gctacccgcc gcaagggcct
      121 cctcgagtcc gggggcatgc cgggcaaact ccgcgactgt cagagccgga acccggcgga
      181 gtccgagatc tacatcgtgg agggagactc ggccggcggc tcggccgtcc ggggacgcga
      241 cccccagacc caggcgatcc ttccgcttcg cgggaagatt ctcaacgtgg aacgagcccg
      301 gctggacagg gcgcttggca acaacgaggt ccaggcgctc attacggcgt tcggcacggg
      361 catcggcgag gacttcgatc tggccaagtg ccggtaccac aagatcatcc tcatggccga
      421 tgccgacgtc gacggccaac acatccggac gctcctcctc accctcctgt tccgatacat
      481 gcgccccctg atcgacgcgg ggtacgtgta cctcgcgcag cctccgctgt tcaagatcaa
      541 gtggtcgaac aacgaccacg agtacgtctt ttcggatcgc gagagggatg cgctcctcgc
      601 cgatggggcg aaggcgggaa agaagatccc gaaggacaac gcgatccagc ggtacaaggg
      661 cctcggcgag atggactact ccgaactgtg ggagacgacc atgaacccgg tgtcgcgaac
      721 gctcctccag gtcaccatgg acgacgcggc ggcggcggac gaagtgtttt ctgtactcat
      781 gggcgaggac gttgagtccc gccggtcgtt catccagcgg aacgcgaagg atgtgagatt
      841 ccttgacatc tgaggtgacg ggtcacgaca agatcgagcc catcgacatc caggtcgaga
      901 tgcagcgctc gtacctcgac tacgcgatgt cggtcatcgt cgggcgcgcg ttgccggagg
      961 ttcgcgacgg gctgaagccc gtccaccgcc gcgtcatcta cgcgatgttt gacgggggct
     1021 accgccccga ccgtgcgtac tccaagtgct cgcgggttgt cggcgacgtc atgggcaagt
     1081 atcacccgca cggcgactct gcgatctacg acaccctcgt ccgcctggtc caggactggt
     1141 ctcttcgcta ccccctcgtc gacggccagg gcaacttcgg tagccccggc aacgacccgg
     1201 ccgccgcacc gcggtacacc gagtgtcgaa tggccccact cgccatggag atggtgcggg
     1261 acatcgacaa ggacaccgtc gacttccagg acaactacga cggccggacg caggaaccgt
     1321 cgatccttcc ggcgcgcttc ccgaacctgc tcgtcaacgg ctcggccggc atcgccgtcg
     1381 gcatggcgac gaacatcccg ccccacaacc tgcgcgaggt cgcctcgggg gcgcagtggt
     1441 tcctggagaa cccggacgcg tcgaaggagg tactgcttga ggcactgatc gaacgaatca
     1501 agggcccgga cttccccaca ggggcgatga tcctcgggcg cgccggcatc gatcaggcgt
     1561 atcgcaccgg acgcggctcc attaccatgc gcgccgtcgt cgaggtcgag gagatccagg
     1621 gtcgcatgtg cctggtcgtc agcgagttgc cctaccaggt caaccccgac aaccttgcgc
     1681 agaagatcgc cgagttggtc cgcgacggca agattgccgg catcgccgac atccgcgacg
     1741 agacgtccgg ccggacgggc cagcggctcg tcatcgtgct caagcgcgac gcggttgcca
     1801 aagtggtcct caacaacctc tacaagcaca cccagcttca ggacaacttc ggcgcgaaca
     1861 tgctcgcgct cgtcgatggc gttccgcgca cgctcaccct cgacgcgttc attcggcact
     1921 gggtcgcgca ccagatcgag gtcatcgtcc gacggaccca attccgtctg cgcgaagccg
     1981 agcgcgagat ccacatcctt cgcggctacc tcaaggccct cgacgccctc gacgccgtga
     2041 tcgcgctcat ccgggcggcc gcgacggtcg acgaggcccg cgaacgcctc atgacgttcc
     2101 tggacgtcga cgagatccag gcgaatgcca tcctcaacct tcaactccgg cgcctcgccg
     2161 ccctcgagcg gaagaagatc gaggatgacc acgcttccct cgagtcggag atctcggacc
     2221 tcaacaacat cctcgcgtcg gaggcgcgcc agcggacaat catcgcgacg gaactcggcg
     2281 agatcgttgc gaagtacggc gacgagcgcc ggaccatcat cgccccccac gagggcgaca
     2341 tggacatcga ggacctcatc gccgaggaag aggtggtcgt cacgattacc cgctccgggt
     2401 acgcgaagcg aaccaagtcg tcgctttacc gctctcagaa gcggggaggc aagggggtcc
     2461 gcggtgcgag cctgagggcg gacgacgtgg tccagcactt cttcgtgacg accacccacc
     2521 actggctcct gttcttcacg aacctcggca gggtgtatcg ggccaaggcg tacgagcttc
     2581 cggatggcgc gcgcgacgcc aagggtcagc acgtcgcgaa catgcttgcc ttccagccgg
     2641 gcgaggagat tgcggaggtc ctcgacctca agacgtatgc cgacaagcca tacctcgttc
     2701 tcgcgaccaa gcgaggcctc gtcaagaaga cgaggctgga ggagtacgac tcgaaccgct
     2761 ccggcggcct catcgccatc aacctccggc ccatcgggag agaggaagac gcggtcgcgg
     2821 acgacgctcc aaccggcgtc gcgctgaccg acgaactcat ggcggctcgg ctcgtgtccg
     2881 acgaggacga cctgatgctg gtgagccgca agggtcagtc gattcgcttc accgccgacg
     2941 gcgacgcgct ccggcccatg ggccgggcca cctccggcgt cacggggatg cgcttccgcg
     3001 aggatgacga gctgctcgcc atggacgtcg ttcgcgacgg gatgttcagt ttcgtcgcga
     3061 ccgagggagg atatgcgaag cggacggacg tggcggagta ccgaagccag ggacgcggtg
     3121 ggctcggcat caaggtcgcc aaactccagg aggagcgcgg cgatctcgtc ggcgcgctcc
     3181 tcgtcgacga ggccgacgag gtgctggtcg tcatggaaaa cgggaaggtc atgcgcgcgg
     3241 acgtcgtcgg cgtccccgcc aaggggcgcg acacgatggg cgtgatcttc gccaagccgg
     3301 acgagggtga ccggatcgtg gcgatcgcac gcaacagcga gcggagcctc gacggcgacg
     3361 gtgcggatga tgcgacgggt gacgcggccg cgagcgcggc ggctccgggc gcacgagttt
     3421 cggacgaggg cgcgacggac gaggcctagt tcagttcgcc ccgggaaacc atccggctcg
     3481 tctgggcgtt gatgaggcgc agggggaagg ccttccggcg tgaccatctc ggggcgagcg
     3541 gttaggctga gcacaagcca cgcgcgaccg cgcacgatcg aggagcttgc atgaatgcgg
     3601 actaccccgc caacccggcc acaacgggag cgcacccctt cccgggcgtg acgcaagccg
     3661 tgcctcaggg tccgggtggg ccggaggttg cacccgcgcc gtggggcgcc gctggcgcgc
     3721 gcatgcagtc ccagccaggg gcgggcggtg gcccggtgca cccgggtgcg cccgcggtgc
     3781 ccccgcccag cccgtacgcg gcaccgggag gctcgacggg ctcggcgaat cccggttcgc
     3841 cgtcgcaacc ggtcgcaccc gccgtaccgg ccgcgaaccc gccgacgcag cacgccacaa
     3901 cgccacacac gactgcgcca cacacgactg cgccacacac gactgcgcca cacacga
//
LOCUS       DASVKP010000245         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_161850, whole genome shotgun sequence.
ACCESSION   DASVKP010000245 DASVKP010000000
VERSION     DASVKP010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161850"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..409)
                     /locus_tag="VF362_04900"
     CDS             complement(260..409)
                     /locus_tag="VF362_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590301.1"
                     /translation="MRTPAHHSEGTQTMGEMDIERTVEPSIDEMTDDDGEGRDGADES
                     RGEDA"
     gene            complement(769..1401)
                     /locus_tag="VF362_04905"
     CDS             complement(769..1401)
                     /locus_tag="VF362_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7590302.1"
                     /translation="MAREAHFRRPVMLDMDEADQLQGDIDPADRALIAHETAAAIVSQ
                     ARASRQPDLVARLVALVDEEGIDTVAMMWSKAEPLTLPGALWRLYMLREWVRFDAESV
                     ARSYDHGIHAAGVRHAIAGVEVPPGPAEVKKLADDVLSGVFAGDLAVALDRAGAFCRV
                     AATGAAFEAESDAGHADDRAHQTTLRASQLLRTGEDLERCATMWREEKLD"
     gene            complement(1414..1860)
                     /gene="msrB"
                     /locus_tag="VF362_04910"
     CDS             complement(1414..1860)
                     /gene="msrB"
                     /locus_tag="VF362_04910"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX7590303.1"
                     /translation="MAKIIRTDDEWRAQLTDDQYRALRENGTEPAWTGDLLENKAPGV
                     YACAACGAELFKSSTKFDSGSGWPSFYDVVDPDAVTLITDISYGMARTEVRCAACDSH
                     LGHVFPDGHGTPTGERYCVNSVCLAFEEGDDPDAAAAVLGDPEQLK"
BASE COUNT          287 a          733 c          726 g          363 t
ORIGIN      
        1 ccccttcgct tcctcggcct tgaccttggc gccctcagcc ttgaggaaga ggtcgtcctt
       61 cgtcgtattc ggggtgctca tgagtatccg gtgctccttg gcttgtgtcg aagcccagcg
      121 gcgctgcttc gcttaccacc cactctatcc ccgacggcga tcacatggct cctggcccga
      181 agtgaacact gggcgaacga gggcgccgcc cacgctccgg cgagcggcgc cctacgatgc
      241 ggtctccggc gagatgcggc taggcgtctt cgccgcgact ctcgtccgcg ccgtcgcggc
      301 cctcgccgtc gtcgtccgtc atctcgtcga tgctcggctc gaccgtccgc tcgatgtcca
      361 tttcgcccat ggtctgggtc ccttcggagt ggtgcgcagg agtgcgcacg tcggacgcta
      421 cgcgcgggag gtgacggcga cctgaacagt ttcgaaacgt ttgatggacc gcgcgcttcg
      481 tacgacgatg tggggacccc gcgaggggcc gagccgggga aggatttgac atcggaccgg
      541 gagcgccagt cggcgcttgg gccgctcgaa gcggcttgag ttggagcccg gggctaccgc
      601 ggtccgatgt ctactccagg gtaactccgg cgacgccctg ttgccacaaa acgtgagaat
      661 cgtaacgttg gcgcggggac ggccgcgcca gacgccgggc cgagcggcgt gtacgcgagg
      721 cccggcgcaa cgccggagcg ggcggtgggg acgcccgcgc cagacaactc agtcgagttt
      781 ctcctcgcgc cacatggtgg cgcatcgctc caggtcctcg ccggtgcgaa gcaattggga
      841 cgcccggagg gtcgtctggt gggcacggtc gtcggcgtgg cccgcgtcgc tctccgcctc
      901 gaacgccgca ccggtcgcgg cgacgcggca gaaggccccc gcgcggtcga gcgccacggc
      961 gaggtcgccc gcgaacactc cgctcaggac gtcgtcggcg agcttcttca cctcggcggg
     1021 cccgggagga acctcgaccc cggcgatggc atgccggacg ccagccgcgt ggatgccgtg
     1081 gtcgtacgag cgcgccaccg actccgcgtc gaatctcacc cactcgcgga gcatgtagag
     1141 gcgccacagg gcgccgggca gcgtgagcgg ctcggccttg gaccacatca tcgcgacggt
     1201 gtcgatgccc tcctcatcca cgagcgcgac gagcctggcg accaggtcgg gctggcggct
     1261 cgcgcgggcc tgcgacacga tcgccgcggc cgtctcgtgc gcgatcagag cgcggtcggc
     1321 cgggtcgata tcgccctgga gctggtcggc ctcgtccatg tcgagcatga ccggcctccg
     1381 gaaatgcgcc tcgcgcgcca tccgtcgtcc ttcctacttc agttgttctg gatcgcccag
     1441 tacagcggcc gccgcgtccg ggtcgtcgcc ctcttcgaag gcgaggcaga cgctgttgac
     1501 gcagtaccgc tcgcccgtgg gcgtgccgtg cccgtcgggg aagacgtgac ccaggtgcga
     1561 gtcgcacgcg gcgcaacgaa cctcggtgcg cgccattccg taggagatgt ccgtgatgag
     1621 cgtgacggcg tcggggtcga cgacgtcgta gaagctgggc cagccggagc cggagtcaaa
     1681 cttggtgctc gacttgaaca gttcggctcc gcacgccgcg caggcgtaca cgccgggcgc
     1741 cttgttttca agcaggtcac cggtccacgc tggctcggtg ccgttctcgc ggagcgcacg
     1801 gtactggtcg tccgtgagtt gggcgcgcca ctcgtcgtcg gtcctgatga tcttcgccat
     1861 gggggtctcc ttccggacct cctcccaggg taggggaggg ggtgaccgcc gcggctcacg
     1921 ccgttgcggg gccctagata ggacgccgcg ggcgggcgtt tgttccctcg cggctaccta
     1981 tgcgctccat gggccattcg tcaacgtgtt gactagcctg gtaccaacgg aaggatccgc
     2041 catgcgcctc gcaccgctcg caggctccgt acccctcgct cgcctcatgc ccctctctgc
     2101 cctcgcggc
//
LOCUS       DASVKP010000246         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Demequinaceae bacterium isolate SMAG_U3049
            k141_328708, whole genome shotgun sequence.
ACCESSION   DASVKP010000246 DASVKP010000000
VERSION     DASVKP010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959680
            Sequence Read Archive: SRR10489834
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Demequinaceae bacterium (soil metagenome)
  ORGANISM  Demequinaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
            Demequinaceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 23:29:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 985
            CDSs (total)                      :: 967
            Genes (coding)                    :: 962
            CDSs (with protein)               :: 962
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 2 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Demequinaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328708"
                     /isolate="SMAG_U3049"
                     /isolation_source="soil"
                     /db_xref="taxon:2683256"
                     /environmental_sample
                     /country="Russia: Chersky"
                     /lat_lon="68.6125 N 161.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VF362_04915"
     CDS             <1..797
                     /locus_tag="VF362_04915"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX7590304.1"
                     /translation="AWTPGVYQGAPTPVTAFMAAATKAAAAAALARLLYLALFGMAWE
                     IAPVLWTVAIATMLLGTVVAIVQTDVKRMLAYSSIAHAGFLLVALTSFRGDALAAVPF
                     YMLAYGLATLGAFAVVSQVRERTPDGALGAEATRLGQWTGLGRRAPWLASAMALFLLS
                     FAGIPLTAGFIGKFVVFKAAIDAGAWPLVLVAVLASAAAAFFYVRLIVLMFLTPVPPE
                     LEESIEVSNATGPWLVMVVAAAGTILLGVLPSPVLDLAHQAAILVP"
     gene            794..1786
                     /locus_tag="VF362_04920"
     CDS             794..1786
                     /locus_tag="VF362_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7590305.1"
                     /translation="MSTHFAIGDLEAVIAPRLKVVEERLRDAVAQSDALAQATSRHLV
                     DAGGKRLRPMLTLLTAQLGDGARPEVLDAAVVVELTHLGTLYHDDVMDSAPTRRGAPS
                     AHQVWGNSVAILTGDLLFARASRVVAGLGPEAVRIQAETFERLCLGQLHETVGPDPGA
                     DPIAHYLQVLADKTGSLIGASARLGAMFAGCERRIVDQVTEYGELAGVAFQLADDVID
                     LRSDAKTTGKTPGTDLREGVATMPVLALRARVAAGADASDAALLDRITGDLADDAALA
                     DVVAELATHSVVDETAALARHWSREARGAIAPLPDGPVKDALLSFADALVARAS"
     gene            1896..>2381
                     /locus_tag="VF362_04925"
     CDS             1896..>2381
                     /locus_tag="VF362_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HEX7590306.1"
                     /translation="MKNFSRLPQHIGIIPDGNRRWAQHKGMNKQDGYESGLRPGLEVY
                     ELCLELGIKELTFYGFTVDNTKRPSAQIRAFRKACVDAVEYLASKDADLLVVGNTQSS
                     FFPKALLPYTERTRCGRGLIKVNFLANYGWNWDLNYAARNGATSSSGNVVDAIASSDI
                     SR"
BASE COUNT          353 a          832 c          793 g          403 t
ORIGIN      
        1 acgcgtggac gcccggcgtt taccagggcg cgcccacgcc ggtcaccgcg ttcatggccg
       61 ccgcgaccaa ggcggctgcg gccgccgcgc tcgcccgcct tctgtacctc gcgctcttcg
      121 ggatggcgtg ggagatcgcc ccggtcctgt ggaccgtagc gatcgccacc atgctgctgg
      181 gcaccgtcgt cgcgatcgtg cagaccgatg tgaagcgcat gctcgcctac tcgtcgatcg
      241 cgcacgcggg cttcctcctc gtggcgctga cctcgttccg cggcgacgcg ctcgccgcgg
      301 tcccgttcta catgcttgcg tacggcctgg cgacgctcgg cgcgtttgcc gtggtgagtc
      361 aggtcaggga gcgcacgccg gatggagcgc tcggcgcaga ggccacgcgg cttggccagt
      421 ggaccgggct tgggcgccgt gcgccctggc tcgcctcggc catggcgctc ttcctcctgt
      481 cgttcgccgg gatcccgctc actgcaggct tcatcggaaa gttcgtggtg ttcaaggccg
      541 cgatcgacgc gggggcctgg ccgctcgtgc tcgtcgccgt gcttgcctcg gcggccgccg
      601 cgttcttcta cgtgcgcctc atcgtgctga tgttcctcac cccggtgccg ccggaactcg
      661 aggaatccat cgaggtcagc aacgcgaccg ggccgtggct cgtcatggtt gtcgcggccg
      721 cggggaccat cctcctcggg gtccttccgt ctcccgtcct tgacttggcg caccaggccg
      781 cgatcctggt tccgtgagca cccatttcgc catcggcgac ctcgaggcgg tcattgcgcc
      841 acgcctcaag gtggtcgagg agcggctccg cgacgcggtc gcccagtccg atgcgctcgc
      901 ccaggccacg agtaggcacc tcgtcgacgc cgggggcaag cgccttcggc ccatgttgac
      961 gcttctgacg gcgcaactcg gggatggcgc ccggcccgag gtgctcgacg cggctgtggt
     1021 cgtcgaactg acacacctcg ggacgctcta tcacgacgac gtcatggact ccgcgccgac
     1081 caggcgcggc gcgccctccg cccaccaagt ctggggcaac tccgtcgcca tcctgacggg
     1141 cgacctcctc ttcgcgcgcg cctcgcgcgt cgtggccggg cttggtcccg aggccgtgcg
     1201 aatccaggcc gagacgttcg agcggctgtg cctcgggcaa ctccatgaga cggtcggccc
     1261 ggacccgggc gccgacccta tcgcgcacta cctccaggtc ctcgccgaca agaccggctc
     1321 cctcatcggc gcgtccgcgc ggcttggggc gatgttcgcg gggtgcgaac gccggatcgt
     1381 tgaccaggtc accgagtacg gcgagctcgc aggggttgcc ttccaactcg ccgacgacgt
     1441 gattgacctt cgctcggacg ccaagacgac cgggaagacg cccggtacgg acctgcgcga
     1501 gggcgtggcg accatgcccg tcctcgcact tcgtgcccgg gttgccgcgg gggcggatgc
     1561 gtcggacgcc gcactgctcg accgcatcac gggggacctc gcggatgacg cggcgcttgc
     1621 cgacgtcgtc gcagaactcg ccacccattc cgtcgtcgac gagacggccg cccttgcccg
     1681 gcactggagt cgcgaggcga ggggggcgat cgcccccctg cccgatggcc ccgtcaagga
     1741 cgcgctccta tcgttcgccg acgccctggt ggcgcgggcg tcctagcctc gggcttccgt
     1801 ggcaggcttg gtcccatgac gcagcaattt gggtggcatc acaacccccg ggaccgcagc
     1861 cggcacatgc agacgagcac aggcggggga cggtcatgaa gaacttcagt cggctcccgc
     1921 aacacatcgg catcattccg gacggaaacc ggaggtgggc tcagcacaag ggaatgaaca
     1981 agcaggacgg gtatgagtcc ggccttcggc caggactcga ggtgtacgaa ctgtgtctag
     2041 agctcgggat aaaggagctg accttctacg ggttcaccgt agacaacacc aaacgcccct
     2101 ccgcgcagat ccgagccttt cggaaggcgt gcgttgacgc cgtcgaatac ctggcaagca
     2161 aggacgcgga cttgctggtc gtcgggaata ctcagtcgtc gttcttcccc aaggcactgt
     2221 tgccgtacac ggagagaacg agatgcgggc gcggtctcat caaggtcaac ttcctggcca
     2281 actatggctg gaactgggac ctcaactacg cggcgcggaa cggcgccacg agttcgagtg
     2341 gcaacgtcgt tgacgccata gcgtcttcgg acatctcaag a
//
